A conjugate of lysozyme with avarone, a bioactive sesquiterpene quinone of marine origin, and its three derivatives were synthesized. MALDI TOF mass spectral analysis and tryptic digestion showed that the only residue in lysozyme that was modified by all derivatives was lysine 97. The identity of the residue was in full correlation with the prediction obtained by molecular modeling. All bioconjugates preserved most of the enzymatic activity of lysozyme. The melting point of the conjugates was slightly increased in comparison to lysozyme, indicating a slight stabilization of structure. The antibacterial activity of all the conjugates to both Gram positive and Gram negative bacteria was stronger than the activity of either lysozyme or the quinones, the MIC values being in low micromolar range for some conjugates.
This is the peer-reviewed version of the article: Novaković, I., Anđelković, U., Zlatović, M., Gašić, M.J., Sladić, D., 2012. Bioconjugate of Lysozyme and the Antibacterial Marine Sesquiterpene Quinone Avarone and Its Derivatives. Bioconjugate Chem. 23, 57–65. https://doi.org/10.1021/bc200330m
TY - JOUR
AU - Novaković, Irena
AU - Anđelković, Uroš
AU - Zlatović, Mario
AU - Gašić, Miroslav J.
AU - Sladić, Dušan
PY - 2012
UR - https://cer.ihtm.bg.ac.rs/handle/123456789/1005
AB - A conjugate of lysozyme with avarone, a bioactive sesquiterpene quinone of marine origin, and its three derivatives were synthesized. MALDI TOF mass spectral analysis and tryptic digestion showed that the only residue in lysozyme that was modified by all derivatives was lysine 97. The identity of the residue was in full correlation with the prediction obtained by molecular modeling. All bioconjugates preserved most of the enzymatic activity of lysozyme. The melting point of the conjugates was slightly increased in comparison to lysozyme, indicating a slight stabilization of structure. The antibacterial activity of all the conjugates to both Gram positive and Gram negative bacteria was stronger than the activity of either lysozyme or the quinones, the MIC values being in low micromolar range for some conjugates.
PB - American Chemical Society (ACS)
T2 - Bioconjugate Chemistry
T1 - Bioconjugate of Lysozyme and the Antibacterial Marine Sesquiterpene Quinone Avarone and Its Derivatives
VL - 23
IS - 1
SP - 57
EP - 65
DO - 10.1021/bc200330m
ER -
@article{
author = "Novaković, Irena and Anđelković, Uroš and Zlatović, Mario and Gašić, Miroslav J. and Sladić, Dušan",
year = "2012",
abstract = "A conjugate of lysozyme with avarone, a bioactive sesquiterpene quinone of marine origin, and its three derivatives were synthesized. MALDI TOF mass spectral analysis and tryptic digestion showed that the only residue in lysozyme that was modified by all derivatives was lysine 97. The identity of the residue was in full correlation with the prediction obtained by molecular modeling. All bioconjugates preserved most of the enzymatic activity of lysozyme. The melting point of the conjugates was slightly increased in comparison to lysozyme, indicating a slight stabilization of structure. The antibacterial activity of all the conjugates to both Gram positive and Gram negative bacteria was stronger than the activity of either lysozyme or the quinones, the MIC values being in low micromolar range for some conjugates.",
publisher = "American Chemical Society (ACS)",
journal = "Bioconjugate Chemistry",
title = "Bioconjugate of Lysozyme and the Antibacterial Marine Sesquiterpene Quinone Avarone and Its Derivatives",
volume = "23",
number = "1",
pages = "57-65",
doi = "10.1021/bc200330m"
}
Novaković, I., Anđelković, U., Zlatović, M., Gašić, M. J.,& Sladić, D.. (2012). Bioconjugate of Lysozyme and the Antibacterial Marine Sesquiterpene Quinone Avarone and Its Derivatives. in Bioconjugate Chemistry
American Chemical Society (ACS)., 23(1), 57-65.
https://doi.org/10.1021/bc200330m
Novaković I, Anđelković U, Zlatović M, Gašić MJ, Sladić D. Bioconjugate of Lysozyme and the Antibacterial Marine Sesquiterpene Quinone Avarone and Its Derivatives. in Bioconjugate Chemistry. 2012;23(1):57-65.
doi:10.1021/bc200330m .
Novaković, Irena, Anđelković, Uroš, Zlatović, Mario, Gašić, Miroslav J., Sladić, Dušan, "Bioconjugate of Lysozyme and the Antibacterial Marine Sesquiterpene Quinone Avarone and Its Derivatives" in Bioconjugate Chemistry, 23, no. 1 (2012):57-65,
https://doi.org/10.1021/bc200330m . .
