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Detection and quantification of leucyl arninopeptidase after native electrophoresis using leucine-p-nitroanilide
(Wiley-V C H Verlag Gmbh, Weinheim, 2005)
A general method for detecting leucyl aminopeptidase activity after native polyacrylamide gel electrophoresis (PAGE) in situ is described. The method is based on diazotization of p-nitroaniline, liberated in the polyacrylamide ...