TY  - JOUR
AU  - Milovanović, Slobodan R.
AU  - Oreščanin, Zorana
AU  - Spasić, Snežana
AU  - Miletić, Srđan
AU  - Prostran, Milica
AU  - Spasić, Mihajlo B.
PY  - 2004
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/141
AB  - In this study themolecular foundation of nitric oxide induced relaxation of arteries, with or without endothelium, of normotensive and spontanously hypertensive ratswas re-examined. With this purpose in mind, the effects of the nitric oxide donor sodium nitroprusside (NaNP), with and without manganese containing superoxide dismutase (MnSOD E.C. 1.15.1.1), on rat renal artery relaxation was strudied. The results show that the relaxation effect of NaNP is two times higher in normotensive, compared to spontaneously hypertensive rats. Similar differences exist in the relaxation effects of NaNP on isolated renal arteries without endothelium, indicating that besides the difference in the function of an endothelium, concerning basal NO production in normotensive and hypertensive rats, there is a differencewith respect to NO relaxation in the smoothmuscle that is induced by hypertension. MnSOD decreased the relaxation effect of NaNP in all the examined renal arteries, more in normotensive than in hypertensive ones regardless of the presence of an endothelium. These results show that MnSOD, by modifying the chemical versatility of NO into redox active forms - nitrosonium (NO+) and nitroxyl (NO-), produces different relaxation effects in normotensive and hypertensive arteries of rats, with or without an endothelium, potentiating the role of nitroxyl induced relaxation in sponteneously hypertensive rats. The results prove the need for the synthesis of complex NO donors, as the mechanisms of artery relaxation are different due to an endothel and smooth mouscle changes in hypertensive, as compared to normotensive rats.
AB  - U ovom radu smo pokušali da detaljnije ispitamo molekulsku osnovu azotoksid indukovane relaksacije arterija, sa i bez endotela, normotenzivnih i spontano hipertenzivnih pacova. U tu svrhu ispitivan je efekat azot-oksid (NO) donora natrijum- nitroprusida (NaNP), bez i u prisustvu superoksid-dismutaze koja sadrži mangan (MnSOD, EC 1.15.1.1) na relaksaciju renalne arterije. Rezulati pokazuju dvostruko veći relaksantni efekat NaNP kod normotenzivnih, u odnosu na spontano hipertenzivne pacove. Slična razlika postoji i u relaksantnom efektu NaNP na izolovane renalne arterije normotenzivnog i hipertenzivnog pacova kada je odstranjen endotel što ukazuje da, pored razlike u funciji endotela u odnosu na bazalnu NO produkciju kod normotenzivnih i spontano hipertenzivnih pacova, postoje i promene u glatkim mišićima indukovane hipertenzijom, u odnosu na NO relaksaciju. MnSOD kod svih grupa smanjuje relaksantni efekat SNP i to više kod normotenzivnih nego kod hipertenzivnih nezavisno od prisustva endotela. Ovi rezultati pokazuju da MnSOD menjajući hemijsku prirodu iz NaNP oslobođenog NO u redoks aktivne forme – nitrozonijum (NO+) i nitroksil (NO–) – ostvaruje različit relaksantni efekat kod normotenzivnih i kod hiperetenzivnih pacova sa i bez endotela potencirajući značaj nitroksilom indukovane relaksacije kod spontano-hipertenzivnih pacova. Dobijeni rezultati potvrđuju potrebu sinteze NO donora koji daju različite redoks-aktivne forme NO budući da se primarni mehanismi relaksacije razlikuju u zavisnosti od karakteristika endotela i glatkih mišića, kod hipertenzije u odnosu na normotenzivno stanje.
PB  - Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - Effect of MnSOD (E. coli) on the relaxation caused by sodium nitroprusside on isolated rat renal artery
T1  - Efekat MnSOD (E. coli) Na relaksaciju izolovane renalne arterije pacova izazvanu natrijum-nitro-prusidom
VL  - 69
IS  - 11
SP  - 973
EP  - 980
DO  - 10.2298/JSC0411973M
ER  - 

@article{
author = "Milovanović, Slobodan R. and Oreščanin, Zorana and Spasić, Snežana and Miletić, Srđan and Prostran, Milica and Spasić, Mihajlo B.",
year = "2004",
abstract = "In this study themolecular foundation of nitric oxide induced relaxation of arteries, with or without endothelium, of normotensive and spontanously hypertensive ratswas re-examined. With this purpose in mind, the effects of the nitric oxide donor sodium nitroprusside (NaNP), with and without manganese containing superoxide dismutase (MnSOD E.C. 1.15.1.1), on rat renal artery relaxation was strudied. The results show that the relaxation effect of NaNP is two times higher in normotensive, compared to spontaneously hypertensive rats. Similar differences exist in the relaxation effects of NaNP on isolated renal arteries without endothelium, indicating that besides the difference in the function of an endothelium, concerning basal NO production in normotensive and hypertensive rats, there is a differencewith respect to NO relaxation in the smoothmuscle that is induced by hypertension. MnSOD decreased the relaxation effect of NaNP in all the examined renal arteries, more in normotensive than in hypertensive ones regardless of the presence of an endothelium. These results show that MnSOD, by modifying the chemical versatility of NO into redox active forms - nitrosonium (NO+) and nitroxyl (NO-), produces different relaxation effects in normotensive and hypertensive arteries of rats, with or without an endothelium, potentiating the role of nitroxyl induced relaxation in sponteneously hypertensive rats. The results prove the need for the synthesis of complex NO donors, as the mechanisms of artery relaxation are different due to an endothel and smooth mouscle changes in hypertensive, as compared to normotensive rats., U ovom radu smo pokušali da detaljnije ispitamo molekulsku osnovu azotoksid indukovane relaksacije arterija, sa i bez endotela, normotenzivnih i spontano hipertenzivnih pacova. U tu svrhu ispitivan je efekat azot-oksid (NO) donora natrijum- nitroprusida (NaNP), bez i u prisustvu superoksid-dismutaze koja sadrži mangan (MnSOD, EC 1.15.1.1) na relaksaciju renalne arterije. Rezulati pokazuju dvostruko veći relaksantni efekat NaNP kod normotenzivnih, u odnosu na spontano hipertenzivne pacove. Slična razlika postoji i u relaksantnom efektu NaNP na izolovane renalne arterije normotenzivnog i hipertenzivnog pacova kada je odstranjen endotel što ukazuje da, pored razlike u funciji endotela u odnosu na bazalnu NO produkciju kod normotenzivnih i spontano hipertenzivnih pacova, postoje i promene u glatkim mišićima indukovane hipertenzijom, u odnosu na NO relaksaciju. MnSOD kod svih grupa smanjuje relaksantni efekat SNP i to više kod normotenzivnih nego kod hipertenzivnih nezavisno od prisustva endotela. Ovi rezultati pokazuju da MnSOD menjajući hemijsku prirodu iz NaNP oslobođenog NO u redoks aktivne forme – nitrozonijum (NO+) i nitroksil (NO–) – ostvaruje različit relaksantni efekat kod normotenzivnih i kod hiperetenzivnih pacova sa i bez endotela potencirajući značaj nitroksilom indukovane relaksacije kod spontano-hipertenzivnih pacova. Dobijeni rezultati potvrđuju potrebu sinteze NO donora koji daju različite redoks-aktivne forme NO budući da se primarni mehanismi relaksacije razlikuju u zavisnosti od karakteristika endotela i glatkih mišića, kod hipertenzije u odnosu na normotenzivno stanje.",
publisher = "Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Effect of MnSOD (E. coli) on the relaxation caused by sodium nitroprusside on isolated rat renal artery, Efekat MnSOD (E. coli) Na relaksaciju izolovane renalne arterije pacova izazvanu natrijum-nitro-prusidom",
volume = "69",
number = "11",
pages = "973-980",
doi = "10.2298/JSC0411973M"
}

Milovanović, S. R., Oreščanin, Z., Spasić, S., Miletić, S., Prostran, M.,& Spasić, M. B.. (2004). Effect of MnSOD (E. coli) on the relaxation caused by sodium nitroprusside on isolated rat renal artery. in Journal of the Serbian Chemical Society
Serbian Chemical Society., 69(11), 973-980.
https://doi.org/10.2298/JSC0411973M

Milovanović SR, Oreščanin Z, Spasić S, Miletić S, Prostran M, Spasić MB. Effect of MnSOD (E. coli) on the relaxation caused by sodium nitroprusside on isolated rat renal artery. in Journal of the Serbian Chemical Society. 2004;69(11):973-980.
doi:10.2298/JSC0411973M .

Milovanović, Slobodan R., Oreščanin, Zorana, Spasić, Snežana, Miletić, Srđan, Prostran, Milica, Spasić, Mihajlo B., "Effect of MnSOD (E. coli) on the relaxation caused by sodium nitroprusside on isolated rat renal artery" in Journal of the Serbian Chemical Society, 69, no. 11 (2004):973-980,
https://doi.org/10.2298/JSC0411973M . .

TY  - JOUR
AU  - Radojičić, Ratko M.
AU  - Spasić, Snežana
AU  - Saičić, Zorica S.
AU  - Jovanović, Tamara B.
AU  - Simić-Krstić, Jovana B.
PY  - 2004
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/155
AB  - The C3 clone of B-16 mouse melanoma was cultured for 1, 6 and 9 days and analyzed. The changes which are not directly linked to melanogenesis in the B-16 / C3 cultures during their maturation were characterized. Early (1 day) confluent (6 days) and old (9 days) cell cultures are distinguished by their leucine aminopeptidase (LAP) and α-naphthyl acetate esterase (ANAE) isoenzyme patterns. Both quantitative and qualitative changes in LAP and ANAE isoenzyme can be observed during culture maturation. There is an increase in the activity of the enzyme copper, zinc-containing superoxide-dismutase (CuZn SOD). The increaase in the CuZn SOD enzyme activity might be related to B-16/C3 cell melanogenesis and / or to differentiation.
AB  - C3 klonovi B-16 melanoma miša su gajeni u kulturi 1, 6 i 9 dana i potom analizirani. Izvršena je karakterizacija promena B-16/C3 ćelijskih kultura tokom njihove maturacije koje nisu direktno povezane sa melanogenezom. Pokazano je da se kulture u početnoj fazi (1 dan), konfluentnoj fazi (6 dana) i kasnoj fazi (9 dana) ćelijskog rasta razlikuju na osnovu izoenzimskih spektara leucin-aminopeptidaze i alfa-naftil-acetat-esteraze. Tokom razvitka ćelijskih kultura B-16/C3 zapažene su kvantitativne i kvalitativne promene leucin-aminopeptidaze i alfa-naftil-acetat-esteraze u njihovim izoenzimskim spektrima. Utvrđeno je da postoji porast aktivnosti bakar-cink superoksid-dismutaze tokom starenja kulture. Porast aktivnosti bakar-cink superoksid-dismutaze može biti povezan sa melanogenezom i/ili diferencijacijom B-16/C3 ćelija.
PB  - Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - Superoxide dismutase activity as a function of culture aging of B-16 mouse melanoma cells
T1  - Aktivnost superoksid-dismutaze tokom maturacije kulture ćelija b-16 melanoma miša
VL  - 69
IS  - 12
SP  - 1005
EP  - 1011
DO  - 10.2298/JSC0412005R
ER  - 

@article{
author = "Radojičić, Ratko M. and Spasić, Snežana and Saičić, Zorica S. and Jovanović, Tamara B. and Simić-Krstić, Jovana B.",
year = "2004",
abstract = "The C3 clone of B-16 mouse melanoma was cultured for 1, 6 and 9 days and analyzed. The changes which are not directly linked to melanogenesis in the B-16 / C3 cultures during their maturation were characterized. Early (1 day) confluent (6 days) and old (9 days) cell cultures are distinguished by their leucine aminopeptidase (LAP) and α-naphthyl acetate esterase (ANAE) isoenzyme patterns. Both quantitative and qualitative changes in LAP and ANAE isoenzyme can be observed during culture maturation. There is an increase in the activity of the enzyme copper, zinc-containing superoxide-dismutase (CuZn SOD). The increaase in the CuZn SOD enzyme activity might be related to B-16/C3 cell melanogenesis and / or to differentiation., C3 klonovi B-16 melanoma miša su gajeni u kulturi 1, 6 i 9 dana i potom analizirani. Izvršena je karakterizacija promena B-16/C3 ćelijskih kultura tokom njihove maturacije koje nisu direktno povezane sa melanogenezom. Pokazano je da se kulture u početnoj fazi (1 dan), konfluentnoj fazi (6 dana) i kasnoj fazi (9 dana) ćelijskog rasta razlikuju na osnovu izoenzimskih spektara leucin-aminopeptidaze i alfa-naftil-acetat-esteraze. Tokom razvitka ćelijskih kultura B-16/C3 zapažene su kvantitativne i kvalitativne promene leucin-aminopeptidaze i alfa-naftil-acetat-esteraze u njihovim izoenzimskim spektrima. Utvrđeno je da postoji porast aktivnosti bakar-cink superoksid-dismutaze tokom starenja kulture. Porast aktivnosti bakar-cink superoksid-dismutaze može biti povezan sa melanogenezom i/ili diferencijacijom B-16/C3 ćelija.",
publisher = "Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Superoxide dismutase activity as a function of culture aging of B-16 mouse melanoma cells, Aktivnost superoksid-dismutaze tokom maturacije kulture ćelija b-16 melanoma miša",
volume = "69",
number = "12",
pages = "1005-1011",
doi = "10.2298/JSC0412005R"
}

Radojičić, R. M., Spasić, S., Saičić, Z. S., Jovanović, T. B.,& Simić-Krstić, J. B.. (2004). Superoxide dismutase activity as a function of culture aging of B-16 mouse melanoma cells. in Journal of the Serbian Chemical Society
Serbian Chemical Society., 69(12), 1005-1011.
https://doi.org/10.2298/JSC0412005R

Radojičić RM, Spasić S, Saičić ZS, Jovanović TB, Simić-Krstić JB. Superoxide dismutase activity as a function of culture aging of B-16 mouse melanoma cells. in Journal of the Serbian Chemical Society. 2004;69(12):1005-1011.
doi:10.2298/JSC0412005R .

Radojičić, Ratko M., Spasić, Snežana, Saičić, Zorica S., Jovanović, Tamara B., Simić-Krstić, Jovana B., "Superoxide dismutase activity as a function of culture aging of B-16 mouse melanoma cells" in Journal of the Serbian Chemical Society, 69, no. 12 (2004):1005-1011,
https://doi.org/10.2298/JSC0412005R . .