TY  - JOUR
AU  - Arsenijević, Dragana
AU  - Stojanović, Bojana
AU  - Milovanović, Jelena
AU  - Arsenijević, Aleksandar
AU  - Simić, Miloš
AU  - Pergal, Marija
AU  - Kodranov, Igor
AU  - Cvetković, Olga
AU  - Vojvodić, Danilo
AU  - Ristanović, Elizabeta
AU  - Manojlović, Dragan
AU  - Milovanović, Marija
AU  - Arsenijević, Nebojša
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4835
AB  - The main biologically active components of plants belonging to the genus Allium, responsible for their biological activities, including anti-inflammatory, antioxidant and immunomodulatory, are organosulfur compounds. The aim of this study was to synthetize the mixture of dipropyl polysul-fides (DPPS) and to test their biological activity in acute hepatitis. C57BL/6 mice were administered orally with DPPS 6 h before intravenous injection of Concanavalin A (ConA). Liver inflammation, necrosis and hepatocytes apoptosis were determined by histological analyses. Cytokines in liver tissue were determined by ELISA, expression of adhesive molecules and enzymes by RT PCR, while liver mononuclear cells were analyzed by flow cytometry. DPPS pretreatment significantly attenuated liver inflammation and injury, as evidenced by biochemical and histopathological observations. In DPPS-pretreated mice, messenger RNA levels of adhesion molecules and NADPH oxidase complex were significantly reduced, while the expression of SOD enzymes was enhanced. DPPS pretreatment decreased protein level of inflammatory cytokines and increased percentage of T regulatory cells in the livers of ConA mice. DPPS showed hepatoprotective effects in ConA-induced hepatitis, charac-terized by attenuation of inflammation and affection of Th17/Treg balance in favor of T regulatory cells and implicating potential therapeutic usage of DPPS mixture in inflammatory liver diseases.
PB  - MDPI
T2  - Nutrients
T1  - Hepatoprotective effect of mixture of dipropyl polysulfides in concanavalin a-induced hepatitis
VL  - 13
IS  - 3
SP  - 1
EP  - 15
DO  - 10.3390/nu13031022
ER  - 

@article{
author = "Arsenijević, Dragana and Stojanović, Bojana and Milovanović, Jelena and Arsenijević, Aleksandar and Simić, Miloš and Pergal, Marija and Kodranov, Igor and Cvetković, Olga and Vojvodić, Danilo and Ristanović, Elizabeta and Manojlović, Dragan and Milovanović, Marija and Arsenijević, Nebojša",
year = "2021",
abstract = "The main biologically active components of plants belonging to the genus Allium, responsible for their biological activities, including anti-inflammatory, antioxidant and immunomodulatory, are organosulfur compounds. The aim of this study was to synthetize the mixture of dipropyl polysul-fides (DPPS) and to test their biological activity in acute hepatitis. C57BL/6 mice were administered orally with DPPS 6 h before intravenous injection of Concanavalin A (ConA). Liver inflammation, necrosis and hepatocytes apoptosis were determined by histological analyses. Cytokines in liver tissue were determined by ELISA, expression of adhesive molecules and enzymes by RT PCR, while liver mononuclear cells were analyzed by flow cytometry. DPPS pretreatment significantly attenuated liver inflammation and injury, as evidenced by biochemical and histopathological observations. In DPPS-pretreated mice, messenger RNA levels of adhesion molecules and NADPH oxidase complex were significantly reduced, while the expression of SOD enzymes was enhanced. DPPS pretreatment decreased protein level of inflammatory cytokines and increased percentage of T regulatory cells in the livers of ConA mice. DPPS showed hepatoprotective effects in ConA-induced hepatitis, charac-terized by attenuation of inflammation and affection of Th17/Treg balance in favor of T regulatory cells and implicating potential therapeutic usage of DPPS mixture in inflammatory liver diseases.",
publisher = "MDPI",
journal = "Nutrients",
title = "Hepatoprotective effect of mixture of dipropyl polysulfides in concanavalin a-induced hepatitis",
volume = "13",
number = "3",
pages = "1-15",
doi = "10.3390/nu13031022"
}

Arsenijević, D., Stojanović, B., Milovanović, J., Arsenijević, A., Simić, M., Pergal, M., Kodranov, I., Cvetković, O., Vojvodić, D., Ristanović, E., Manojlović, D., Milovanović, M.,& Arsenijević, N.. (2021). Hepatoprotective effect of mixture of dipropyl polysulfides in concanavalin a-induced hepatitis. in Nutrients
MDPI., 13(3), 1-15.
https://doi.org/10.3390/nu13031022

Arsenijević D, Stojanović B, Milovanović J, Arsenijević A, Simić M, Pergal M, Kodranov I, Cvetković O, Vojvodić D, Ristanović E, Manojlović D, Milovanović M, Arsenijević N. Hepatoprotective effect of mixture of dipropyl polysulfides in concanavalin a-induced hepatitis. in Nutrients. 2021;13(3):1-15.
doi:10.3390/nu13031022 .

Arsenijević, Dragana, Stojanović, Bojana, Milovanović, Jelena, Arsenijević, Aleksandar, Simić, Miloš, Pergal, Marija, Kodranov, Igor, Cvetković, Olga, Vojvodić, Danilo, Ristanović, Elizabeta, Manojlović, Dragan, Milovanović, Marija, Arsenijević, Nebojša, "Hepatoprotective effect of mixture of dipropyl polysulfides in concanavalin a-induced hepatitis" in Nutrients, 13, no. 3 (2021):1-15,
https://doi.org/10.3390/nu13031022 . .

TY  - JOUR
AU  - Đorđević, Dragana B.
AU  - Milovanović, Jelena
AU  - Jurisević, Milena
AU  - Stojanović, Bojana
AU  - Cvetković, Olga
AU  - Pergal, Marija
AU  - Ristanović, Elizabeta
AU  - Vojvodić, Danilo
AU  - Simić, Miloš
AU  - Manojlović, Dragan
AU  - Milovanović, Marija
AU  - Arsenijević, Nebojsa
PY  - 2019
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3421
AB  - Copper serves as a limiting factor for multiple steps of tumour progression, including angiogenesis, growth and metastasis. High levels of copper have been found in a wide spectrum of human cancers. Antitumour activities of copper-chelating drugs have been reported in animal models. Organosulfur compounds (diallyl sulfide, DAS; diallyl disulfide, DADS; S-ethylcysteine, SEC; N-acetylcysteine, NAC) derived from garlic exhibit marked copper-chelating activity. We analysed a mixture of fifteen n-propyl polysulfides (DPPS) for potential antitumour activity against several murine tumour cell lines, including colon carcinoma (CT26), mammary carcinoma (4T1) and melanoma cell lines (B16F10), and compared the effects with the antiproliferative effect in highly proliferative murine mesenchymal stem cells (mMSCs). The effects of the mixture of n-propyl polysulfides (100%) on cell viability were determined using MTT assays. Cell apoptosis was analysed using Annexin V-FITC/PI assays.The results of the MTT assays indicate that this standardized mixture of n-propyl polysulfides has a strong, dose-dependent cytotoxic effect against all three of the tested tumour cell lines (CT26, 4T1, B16F10). The cytotoxic effect of the n-propyl polysulfide mixture against the CT26 and B16F10 cell lines was much stronger than that of cisplatin and was significantly weaker in mMSCs, which are non-cancerous and highly proliferative cells, than in cancer cells. Flow cytometric analysis of CT26 and 4T1 cells revealed that apoptosis was not the dominant mechanism of cell death induced by the n-propyl polysulfide mixture. The n-propyl polysulfide mixture exerted highly cytotoxic activity against murine colon carcinoma and melanoma cell lines, but its antiproliferative activity against mMSCs was significantly lower than that of cisplatin.
AB  - Bakar učestvuje u različitim fazama progresije tumora, u angiogenezi, rastu i metastaziranju. Povećane vrednosti bakra u serumu i u tkivu tumora, karakteristika su različitih vrsta tumora kod ljudi. U animalnim eksperimentalnim modelima, supstance (lekovi) koje heliraju bakar ispoljavaju anti-tumorski efekat. Helatori bakra su i organosumporna jedinjenja, izolovana iz belog luka. U ovoj studiji analizirali smo potencijalnu anti-tumorsku aktivnost smeše petnaest različitih n-propil polisulfi da na nekoliko mišjih ćelijskih linija tumora: karcinom kolona (CT26), karcinom dojke (4T1) i melanom (B16F10). Aktivnost ove smeše na tumorskim linijama, uporedili smo sa antiproliferativnim efektom na mezenhimalne matične ćelije miša (engl. murine mesenchymal stem cells, mMSC). Efekat smeše n-propil polisulfi da (100%) na vijabilnost ćelija ispitali smo MTT testom. Apoptozu ćelija smo analizirali koristeći Annexin V-FITC/PI test. Rezultati MTT testa ukazuju da standardizovana smeša n-propil polisulfi da ima jak citotoksični, dozno-zavisni, efekat na sve tri testirane ćelijske linije tumora (CT26, 4T1, B16F10). Smeša n-propil polisulfi da ispoljava izraženiji citotoksični efekat na CT26 i B16F10 linije u odnosu na cisplatinu. Citotoksični efekat ove smeše na mMSC je značajno slabiji poredeći sa efektom cisplatine, što ukazuje na selektivnije dejstvo. Analiza CT26 i 4T1 ćelija protočnom citometrijom pokazala je da apoptoza nije glavni oblik smrti ćelija, koju uzrokuje smeša n-propil polisulfi - da. Smeša n-propil polisulfi da ispoljava jaču citotoksičnu aktivnost na ćelijskim linijama mišjeg karcinoma kolona i melanoma i slabiju aktivnost na mMSC u poređenju sa efektom cisplatine.
T2  - Serbian Journal of Experimental and Clinical Research
T1  - Antitumour effect of a mixture of n-propyl polysulfides In vitro
T1  - Antitumorski efekti smeše n-propil polisulfida in vitro
VL  - 20
IS  - 4
SP  - 295
EP  - 300
DO  - 10.1515/sjecr-2017-0069
ER  - 

@article{
author = "Đorđević, Dragana B. and Milovanović, Jelena and Jurisević, Milena and Stojanović, Bojana and Cvetković, Olga and Pergal, Marija and Ristanović, Elizabeta and Vojvodić, Danilo and Simić, Miloš and Manojlović, Dragan and Milovanović, Marija and Arsenijević, Nebojsa",
year = "2019",
abstract = "Copper serves as a limiting factor for multiple steps of tumour progression, including angiogenesis, growth and metastasis. High levels of copper have been found in a wide spectrum of human cancers. Antitumour activities of copper-chelating drugs have been reported in animal models. Organosulfur compounds (diallyl sulfide, DAS; diallyl disulfide, DADS; S-ethylcysteine, SEC; N-acetylcysteine, NAC) derived from garlic exhibit marked copper-chelating activity. We analysed a mixture of fifteen n-propyl polysulfides (DPPS) for potential antitumour activity against several murine tumour cell lines, including colon carcinoma (CT26), mammary carcinoma (4T1) and melanoma cell lines (B16F10), and compared the effects with the antiproliferative effect in highly proliferative murine mesenchymal stem cells (mMSCs). The effects of the mixture of n-propyl polysulfides (100%) on cell viability were determined using MTT assays. Cell apoptosis was analysed using Annexin V-FITC/PI assays.The results of the MTT assays indicate that this standardized mixture of n-propyl polysulfides has a strong, dose-dependent cytotoxic effect against all three of the tested tumour cell lines (CT26, 4T1, B16F10). The cytotoxic effect of the n-propyl polysulfide mixture against the CT26 and B16F10 cell lines was much stronger than that of cisplatin and was significantly weaker in mMSCs, which are non-cancerous and highly proliferative cells, than in cancer cells. Flow cytometric analysis of CT26 and 4T1 cells revealed that apoptosis was not the dominant mechanism of cell death induced by the n-propyl polysulfide mixture. The n-propyl polysulfide mixture exerted highly cytotoxic activity against murine colon carcinoma and melanoma cell lines, but its antiproliferative activity against mMSCs was significantly lower than that of cisplatin., Bakar učestvuje u različitim fazama progresije tumora, u angiogenezi, rastu i metastaziranju. Povećane vrednosti bakra u serumu i u tkivu tumora, karakteristika su različitih vrsta tumora kod ljudi. U animalnim eksperimentalnim modelima, supstance (lekovi) koje heliraju bakar ispoljavaju anti-tumorski efekat. Helatori bakra su i organosumporna jedinjenja, izolovana iz belog luka. U ovoj studiji analizirali smo potencijalnu anti-tumorsku aktivnost smeše petnaest različitih n-propil polisulfi da na nekoliko mišjih ćelijskih linija tumora: karcinom kolona (CT26), karcinom dojke (4T1) i melanom (B16F10). Aktivnost ove smeše na tumorskim linijama, uporedili smo sa antiproliferativnim efektom na mezenhimalne matične ćelije miša (engl. murine mesenchymal stem cells, mMSC). Efekat smeše n-propil polisulfi da (100%) na vijabilnost ćelija ispitali smo MTT testom. Apoptozu ćelija smo analizirali koristeći Annexin V-FITC/PI test. Rezultati MTT testa ukazuju da standardizovana smeša n-propil polisulfi da ima jak citotoksični, dozno-zavisni, efekat na sve tri testirane ćelijske linije tumora (CT26, 4T1, B16F10). Smeša n-propil polisulfi da ispoljava izraženiji citotoksični efekat na CT26 i B16F10 linije u odnosu na cisplatinu. Citotoksični efekat ove smeše na mMSC je značajno slabiji poredeći sa efektom cisplatine, što ukazuje na selektivnije dejstvo. Analiza CT26 i 4T1 ćelija protočnom citometrijom pokazala je da apoptoza nije glavni oblik smrti ćelija, koju uzrokuje smeša n-propil polisulfi - da. Smeša n-propil polisulfi da ispoljava jaču citotoksičnu aktivnost na ćelijskim linijama mišjeg karcinoma kolona i melanoma i slabiju aktivnost na mMSC u poređenju sa efektom cisplatine.",
journal = "Serbian Journal of Experimental and Clinical Research",
title = "Antitumour effect of a mixture of n-propyl polysulfides In vitro, Antitumorski efekti smeše n-propil polisulfida in vitro",
volume = "20",
number = "4",
pages = "295-300",
doi = "10.1515/sjecr-2017-0069"
}

Đorđević, D. B., Milovanović, J., Jurisević, M., Stojanović, B., Cvetković, O., Pergal, M., Ristanović, E., Vojvodić, D., Simić, M., Manojlović, D., Milovanović, M.,& Arsenijević, N.. (2019). Antitumour effect of a mixture of n-propyl polysulfides In vitro. in Serbian Journal of Experimental and Clinical Research, 20(4), 295-300.
https://doi.org/10.1515/sjecr-2017-0069

Đorđević DB, Milovanović J, Jurisević M, Stojanović B, Cvetković O, Pergal M, Ristanović E, Vojvodić D, Simić M, Manojlović D, Milovanović M, Arsenijević N. Antitumour effect of a mixture of n-propyl polysulfides In vitro. in Serbian Journal of Experimental and Clinical Research. 2019;20(4):295-300.
doi:10.1515/sjecr-2017-0069 .

Đorđević, Dragana B., Milovanović, Jelena, Jurisević, Milena, Stojanović, Bojana, Cvetković, Olga, Pergal, Marija, Ristanović, Elizabeta, Vojvodić, Danilo, Simić, Miloš, Manojlović, Dragan, Milovanović, Marija, Arsenijević, Nebojsa, "Antitumour effect of a mixture of n-propyl polysulfides In vitro" in Serbian Journal of Experimental and Clinical Research, 20, no. 4 (2019):295-300,
https://doi.org/10.1515/sjecr-2017-0069 . .

TY  - JOUR
AU  - Konovalov, Bata
AU  - Zivkovic, Marija D.
AU  - Milovanovic, Jelena Z.
AU  - Đorđević, Dragana B.
AU  - Arsenijevic, Aleksandar N.
AU  - Vasic, Ivana R.
AU  - Janjić, Goran
AU  - Franich, Andjela
AU  - Manojlović, Dragan
AU  - Škrivanj, Sandra
AU  - Milovanovic, Marija Z.
AU  - Đuran, Miloš
AU  - Rajkovic, Snezana
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2353
AB  - The synthesis, spectroscopic characterization, cytotoxic activity and DNA binding evaluation of seven new dinuclear platinum(ii) complexes Pt1-Pt7, with the general formula [{Pt(L)Cl}(2)(-1,5-nphe)](ClO4)(2) (1,5-nphe is 1,5-naphthyridine; while L is two ammines (Pt1) or one bidentate coordinated diamine: ethylenediamine (Pt2), (+/-)-1,2-propylenediamine (Pt3), trans-(+/-)-1,2-diaminocyclohexane (Pt4), 1,3-propylenediamine (Pt5), 2,2-dimethyl-1,3-propylenediamine (Pt6), and 1,3-pentanediamine (Pt7)), were reported. In vitro cytotoxic activity of these complexes was evaluated against three tumor cell lines, murine colon carcinoma (CT26), murine mammary carcinoma (4T1) and murine lung cancer (LLC1) and two normal cell lines, murine mesenchymal stem cells (MSC) and human fibroblast (MRC-5) cells. The results of the MTT assay indicate that all investigated complexes have almost no cytotoxic effects on 4T1 and very low cytotoxicity toward LLC1 cell lines. In contrast to the effects on LLC1 and 4T1 cells, complexes Pt1 and Pt2 had significant cytotoxic activity toward CT26 cells. Complex Pt1 had a much lower IC50 value for activity on CT26 cells compared with cisplatin. In comparison with cisplatin, all dinuclear Pt1-Pt7 complexes showed lower cytotoxicity toward normal MSC and MRC-5 cells. In order to measure the amount of platinum(ii) complexes taken up by the cells, we quantified the cellular platinum content using inductively coupled plasma mass spectrometry (ICP-QMS). Molecular docking studies performed to evaluate the potential binding mode of dinuclear platinum(ii) complexes Pt1-Pt7 and their aqua derivatives W1-W7, respectively, at the double stranded DNA showed that groove spanning and backbone tracking are the most stable binding modes.
PB  - Royal Soc Chemistry, Cambridge
T2  - Dalton Transactions
T1  - Synthesis, cytotoxic activity and DNA interaction studies of new dinuclear platinum(ii) complexes with an aromatic 1,5-naphthyridine bridging ligand: DNA binding mode of polynuclear platinum(ii) complexes in relation to the complex structure
VL  - 47
IS  - 42
SP  - 15091
EP  - 15102
DO  - 10.1039/c8dt01946k
ER  - 

@article{
author = "Konovalov, Bata and Zivkovic, Marija D. and Milovanovic, Jelena Z. and Đorđević, Dragana B. and Arsenijevic, Aleksandar N. and Vasic, Ivana R. and Janjić, Goran and Franich, Andjela and Manojlović, Dragan and Škrivanj, Sandra and Milovanovic, Marija Z. and Đuran, Miloš and Rajkovic, Snezana",
year = "2018",
abstract = "The synthesis, spectroscopic characterization, cytotoxic activity and DNA binding evaluation of seven new dinuclear platinum(ii) complexes Pt1-Pt7, with the general formula [{Pt(L)Cl}(2)(-1,5-nphe)](ClO4)(2) (1,5-nphe is 1,5-naphthyridine; while L is two ammines (Pt1) or one bidentate coordinated diamine: ethylenediamine (Pt2), (+/-)-1,2-propylenediamine (Pt3), trans-(+/-)-1,2-diaminocyclohexane (Pt4), 1,3-propylenediamine (Pt5), 2,2-dimethyl-1,3-propylenediamine (Pt6), and 1,3-pentanediamine (Pt7)), were reported. In vitro cytotoxic activity of these complexes was evaluated against three tumor cell lines, murine colon carcinoma (CT26), murine mammary carcinoma (4T1) and murine lung cancer (LLC1) and two normal cell lines, murine mesenchymal stem cells (MSC) and human fibroblast (MRC-5) cells. The results of the MTT assay indicate that all investigated complexes have almost no cytotoxic effects on 4T1 and very low cytotoxicity toward LLC1 cell lines. In contrast to the effects on LLC1 and 4T1 cells, complexes Pt1 and Pt2 had significant cytotoxic activity toward CT26 cells. Complex Pt1 had a much lower IC50 value for activity on CT26 cells compared with cisplatin. In comparison with cisplatin, all dinuclear Pt1-Pt7 complexes showed lower cytotoxicity toward normal MSC and MRC-5 cells. In order to measure the amount of platinum(ii) complexes taken up by the cells, we quantified the cellular platinum content using inductively coupled plasma mass spectrometry (ICP-QMS). Molecular docking studies performed to evaluate the potential binding mode of dinuclear platinum(ii) complexes Pt1-Pt7 and their aqua derivatives W1-W7, respectively, at the double stranded DNA showed that groove spanning and backbone tracking are the most stable binding modes.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Dalton Transactions",
title = "Synthesis, cytotoxic activity and DNA interaction studies of new dinuclear platinum(ii) complexes with an aromatic 1,5-naphthyridine bridging ligand: DNA binding mode of polynuclear platinum(ii) complexes in relation to the complex structure",
volume = "47",
number = "42",
pages = "15091-15102",
doi = "10.1039/c8dt01946k"
}

Konovalov, B., Zivkovic, M. D., Milovanovic, J. Z., Đorđević, D. B., Arsenijevic, A. N., Vasic, I. R., Janjić, G., Franich, A., Manojlović, D., Škrivanj, S., Milovanovic, M. Z., Đuran, M.,& Rajkovic, S.. (2018). Synthesis, cytotoxic activity and DNA interaction studies of new dinuclear platinum(ii) complexes with an aromatic 1,5-naphthyridine bridging ligand: DNA binding mode of polynuclear platinum(ii) complexes in relation to the complex structure. in Dalton Transactions
Royal Soc Chemistry, Cambridge., 47(42), 15091-15102.
https://doi.org/10.1039/c8dt01946k

Konovalov B, Zivkovic MD, Milovanovic JZ, Đorđević DB, Arsenijevic AN, Vasic IR, Janjić G, Franich A, Manojlović D, Škrivanj S, Milovanovic MZ, Đuran M, Rajkovic S. Synthesis, cytotoxic activity and DNA interaction studies of new dinuclear platinum(ii) complexes with an aromatic 1,5-naphthyridine bridging ligand: DNA binding mode of polynuclear platinum(ii) complexes in relation to the complex structure. in Dalton Transactions. 2018;47(42):15091-15102.
doi:10.1039/c8dt01946k .

Konovalov, Bata, Zivkovic, Marija D., Milovanovic, Jelena Z., Đorđević, Dragana B., Arsenijevic, Aleksandar N., Vasic, Ivana R., Janjić, Goran, Franich, Andjela, Manojlović, Dragan, Škrivanj, Sandra, Milovanovic, Marija Z., Đuran, Miloš, Rajkovic, Snezana, "Synthesis, cytotoxic activity and DNA interaction studies of new dinuclear platinum(ii) complexes with an aromatic 1,5-naphthyridine bridging ligand: DNA binding mode of polynuclear platinum(ii) complexes in relation to the complex structure" in Dalton Transactions, 47, no. 42 (2018):15091-15102,
https://doi.org/10.1039/c8dt01946k . .

TY  - JOUR
AU  - Vujic, Jelena M.
AU  - Kaluđerović, Goran N.
AU  - Zmejkovski, Bojana
AU  - Milovanovic, Marija
AU  - Volarevic, Vladislav
AU  - Arsenijevic, Nebojsa
AU  - Stanojković, Tatjana
AU  - Trifunovic, Srecko R.
PY  - 2012
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/969
AB  - Synthesis of four new platinum(IV) complexes 1-4, with bidentate N,N'-ligand precursors O,O'-dialkyl esters (alkyl = ethyl, n-propyl, n-butyl and n-pentyl), of (S,S)-ethylenediamine-N,N'-di-2-(4-methyl)pentanoic acid dihydrochloride [(S,S)-H(4)eddl]Cl-2 were reported. The composition of the novel platinum complexes was determined by elemental analysis and characterizations were performed by infrared, H-1 and C-13 NMR spectroscopy. DFT calculations indicate formation one (R,R) from three possible diastereoisomers (S,S; R,S). Complexes 1-4 displayed potent anticancer activity. IC50 values range from 0.74 to 70 mu M, against tested cell lines, except for CLL cells. The antitumoral activity of 2-4 was found to be considerably stronger to Jurkat and K562. Cell cycle analysis of cell lines showed G1 arrest in the presence of analyzed complexes.
PB  - Elsevier Science Sa, Lausanne
T2  - Inorganica Chimica Acta
T1  - Stereospecific ligands and their complexes. Part X: Synthesis, characterization and in vitro antitumoral activity of platinum(IV) complexes with O,O '-dialkyl-(S,S)-ethylenediamine-N,N '-di-2-(4-methyl)pentanoate ligands
VL  - 390
SP  - 123
EP  - 128
DO  - 10.1016/j.ica.2012.03.048
ER  - 

@article{
author = "Vujic, Jelena M. and Kaluđerović, Goran N. and Zmejkovski, Bojana and Milovanovic, Marija and Volarevic, Vladislav and Arsenijevic, Nebojsa and Stanojković, Tatjana and Trifunovic, Srecko R.",
year = "2012",
abstract = "Synthesis of four new platinum(IV) complexes 1-4, with bidentate N,N'-ligand precursors O,O'-dialkyl esters (alkyl = ethyl, n-propyl, n-butyl and n-pentyl), of (S,S)-ethylenediamine-N,N'-di-2-(4-methyl)pentanoic acid dihydrochloride [(S,S)-H(4)eddl]Cl-2 were reported. The composition of the novel platinum complexes was determined by elemental analysis and characterizations were performed by infrared, H-1 and C-13 NMR spectroscopy. DFT calculations indicate formation one (R,R) from three possible diastereoisomers (S,S; R,S). Complexes 1-4 displayed potent anticancer activity. IC50 values range from 0.74 to 70 mu M, against tested cell lines, except for CLL cells. The antitumoral activity of 2-4 was found to be considerably stronger to Jurkat and K562. Cell cycle analysis of cell lines showed G1 arrest in the presence of analyzed complexes.",
publisher = "Elsevier Science Sa, Lausanne",
journal = "Inorganica Chimica Acta",
title = "Stereospecific ligands and their complexes. Part X: Synthesis, characterization and in vitro antitumoral activity of platinum(IV) complexes with O,O '-dialkyl-(S,S)-ethylenediamine-N,N '-di-2-(4-methyl)pentanoate ligands",
volume = "390",
pages = "123-128",
doi = "10.1016/j.ica.2012.03.048"
}

Vujic, J. M., Kaluđerović, G. N., Zmejkovski, B., Milovanovic, M., Volarevic, V., Arsenijevic, N., Stanojković, T.,& Trifunovic, S. R.. (2012). Stereospecific ligands and their complexes. Part X: Synthesis, characterization and in vitro antitumoral activity of platinum(IV) complexes with O,O '-dialkyl-(S,S)-ethylenediamine-N,N '-di-2-(4-methyl)pentanoate ligands. in Inorganica Chimica Acta
Elsevier Science Sa, Lausanne., 390, 123-128.
https://doi.org/10.1016/j.ica.2012.03.048

Vujic JM, Kaluđerović GN, Zmejkovski B, Milovanovic M, Volarevic V, Arsenijevic N, Stanojković T, Trifunovic SR. Stereospecific ligands and their complexes. Part X: Synthesis, characterization and in vitro antitumoral activity of platinum(IV) complexes with O,O '-dialkyl-(S,S)-ethylenediamine-N,N '-di-2-(4-methyl)pentanoate ligands. in Inorganica Chimica Acta. 2012;390:123-128.
doi:10.1016/j.ica.2012.03.048 .

Vujic, Jelena M., Kaluđerović, Goran N., Zmejkovski, Bojana, Milovanovic, Marija, Volarevic, Vladislav, Arsenijevic, Nebojsa, Stanojković, Tatjana, Trifunovic, Srecko R., "Stereospecific ligands and their complexes. Part X: Synthesis, characterization and in vitro antitumoral activity of platinum(IV) complexes with O,O '-dialkyl-(S,S)-ethylenediamine-N,N '-di-2-(4-methyl)pentanoate ligands" in Inorganica Chimica Acta, 390 (2012):123-128,
https://doi.org/10.1016/j.ica.2012.03.048 . .

TY  - JOUR
AU  - Vujic, Jelena M.
AU  - Kaluđerović, Goran N.
AU  - Milovanovic, Marija
AU  - Zmejkovski, Bojana
AU  - Volarevic, Vladislav
AU  - Zivic, Danijela
AU  - Durdevic, Predrag
AU  - Arsenijevic, Nebojsa
AU  - Trifunovic, Srecko R.
PY  - 2011
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/838
AB  - Platinum(II) complexes (1-4) with bidentate N,N'-ligands, O,O'-dialkyl esters (alkyl = ethyl, n-propyl, n-butyl and n-pentyl), of (S,S)-ethylenediamine-N,N'-di-2-(4-methyl)pentanoic acid were synthesized and characterized by IR, (1)H NMR and (13)C NMR spectroscopy and elemental analysis. DFT calculations were performed for the complexes and it was found that only one diastereoisomer could be formed. Cytotoxic activity of complexes 1-4 was determined against chronic lymphocytic leukemia cells (CLL) and compared to the activity of ligand precursors L1 center dot 2HCl-L4 center dot 2HCl and corresponding palladium(II) complexes, [PdCl(2)L] (L = L1-L4). The complexes were found to exhibit significantly higher antitumor activities than cisplatin on CLL cells. Cytotoxic effect of platinum(II) complexes on CLL cells was higher compared to corresponding palladium(II) complexes. In addition the mode of cell death induced by platinum(II) complexes was determined.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux
T2  - European Journal of Medicinal Chemistry
T1  - Stereospecific ligands and their complexes. Part VII. Synthesis, characterization and in vitro antitumoral activity of platinum(II) complexes with O,O '-dialkyl esters of (S,S)-ethylenediamine-N,N '-di-2-(4-methyl)pentanoic acid
VL  - 46
IS  - 9
SP  - 4559
EP  - 4565
DO  - 10.1016/j.ejmech.2011.07.034
ER  - 

@article{
author = "Vujic, Jelena M. and Kaluđerović, Goran N. and Milovanovic, Marija and Zmejkovski, Bojana and Volarevic, Vladislav and Zivic, Danijela and Durdevic, Predrag and Arsenijevic, Nebojsa and Trifunovic, Srecko R.",
year = "2011",
abstract = "Platinum(II) complexes (1-4) with bidentate N,N'-ligands, O,O'-dialkyl esters (alkyl = ethyl, n-propyl, n-butyl and n-pentyl), of (S,S)-ethylenediamine-N,N'-di-2-(4-methyl)pentanoic acid were synthesized and characterized by IR, (1)H NMR and (13)C NMR spectroscopy and elemental analysis. DFT calculations were performed for the complexes and it was found that only one diastereoisomer could be formed. Cytotoxic activity of complexes 1-4 was determined against chronic lymphocytic leukemia cells (CLL) and compared to the activity of ligand precursors L1 center dot 2HCl-L4 center dot 2HCl and corresponding palladium(II) complexes, [PdCl(2)L] (L = L1-L4). The complexes were found to exhibit significantly higher antitumor activities than cisplatin on CLL cells. Cytotoxic effect of platinum(II) complexes on CLL cells was higher compared to corresponding palladium(II) complexes. In addition the mode of cell death induced by platinum(II) complexes was determined.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux",
journal = "European Journal of Medicinal Chemistry",
title = "Stereospecific ligands and their complexes. Part VII. Synthesis, characterization and in vitro antitumoral activity of platinum(II) complexes with O,O '-dialkyl esters of (S,S)-ethylenediamine-N,N '-di-2-(4-methyl)pentanoic acid",
volume = "46",
number = "9",
pages = "4559-4565",
doi = "10.1016/j.ejmech.2011.07.034"
}

Vujic, J. M., Kaluđerović, G. N., Milovanovic, M., Zmejkovski, B., Volarevic, V., Zivic, D., Durdevic, P., Arsenijevic, N.,& Trifunovic, S. R.. (2011). Stereospecific ligands and their complexes. Part VII. Synthesis, characterization and in vitro antitumoral activity of platinum(II) complexes with O,O '-dialkyl esters of (S,S)-ethylenediamine-N,N '-di-2-(4-methyl)pentanoic acid. in European Journal of Medicinal Chemistry
Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux., 46(9), 4559-4565.
https://doi.org/10.1016/j.ejmech.2011.07.034

Vujic JM, Kaluđerović GN, Milovanovic M, Zmejkovski B, Volarevic V, Zivic D, Durdevic P, Arsenijevic N, Trifunovic SR. Stereospecific ligands and their complexes. Part VII. Synthesis, characterization and in vitro antitumoral activity of platinum(II) complexes with O,O '-dialkyl esters of (S,S)-ethylenediamine-N,N '-di-2-(4-methyl)pentanoic acid. in European Journal of Medicinal Chemistry. 2011;46(9):4559-4565.
doi:10.1016/j.ejmech.2011.07.034 .

Vujic, Jelena M., Kaluđerović, Goran N., Milovanovic, Marija, Zmejkovski, Bojana, Volarevic, Vladislav, Zivic, Danijela, Durdevic, Predrag, Arsenijevic, Nebojsa, Trifunovic, Srecko R., "Stereospecific ligands and their complexes. Part VII. Synthesis, characterization and in vitro antitumoral activity of platinum(II) complexes with O,O '-dialkyl esters of (S,S)-ethylenediamine-N,N '-di-2-(4-methyl)pentanoic acid" in European Journal of Medicinal Chemistry, 46, no. 9 (2011):4559-4565,
https://doi.org/10.1016/j.ejmech.2011.07.034 . .