TY  - JOUR
AU  - Mladenović, Minja
AU  - Morgan, Ibrahim
AU  - Ilić, Nebojša
AU  - Saoud, Mohamad
AU  - Pergal, Marija
AU  - Kaluđerović, Goran N.
AU  - Knežević, Nikola Ž.
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4572
AB  - Ruthenium complexes are attracting interest in cancer treatment due to their potent cytotoxic activity. However, as their high toxicity may also affect healthy tissues, efficient and selective drug delivery systems to tumour tissues are needed. Our study focuses on the construction of such drug delivery systems for the delivery of cytotoxic Ru(II) complexes upon exposure to a weakly acidic environment of tumours. As nanocarriers, mesoporous silica nanoparticles (MSN) are utilized, whose surface is functionalized with two types of ligands, (2-thienylmethyl)hydrazine hydrochloride (H1) and (5,6-dimethylthieno[2,3-d]pyrimidin-4-yl)hydrazine (H2), which were attached to MSN through a pH-responsive hydrazone linkage. Further coordination to ruthenium(II) center yielded two types of nanomaterials MSN-H1[Ru] and MSN-H2[Ru]. Spectrophotometric measurements of the drug release kinetics at different pH (5.0, 6.0 and 7.4) confirm the enhanced release of Ru(II) complexes at lower pH values, which is further supported by inductively coupled plasma optical emission spectrometry (ICP-OES) measurements. Furthermore, the cytotoxicity effect of the released metallotherapeutics is evaluated in vitro on metastatic B16F1 melanoma cells and enhanced cancer cell-killing efficacy is demonstrated upon exposure of the nanomaterials to weakly acidic conditions. The obtained results showcase the promising capabilities of the designed MSN nanocarriers for the pH-responsive delivery of metallotherapeutics and targeted treatment of cancer.
PB  - MDPI
T2  - Pharmaceutics
T1  - Ph-responsive release of ruthenium metallotherapeutics from mesoporous silica-based nanocarriers
VL  - 13
IS  - 4
SP  - 460
DO  - 10.3390/pharmaceutics13040460
ER  - 

@article{
author = "Mladenović, Minja and Morgan, Ibrahim and Ilić, Nebojša and Saoud, Mohamad and Pergal, Marija and Kaluđerović, Goran N. and Knežević, Nikola Ž.",
year = "2021",
abstract = "Ruthenium complexes are attracting interest in cancer treatment due to their potent cytotoxic activity. However, as their high toxicity may also affect healthy tissues, efficient and selective drug delivery systems to tumour tissues are needed. Our study focuses on the construction of such drug delivery systems for the delivery of cytotoxic Ru(II) complexes upon exposure to a weakly acidic environment of tumours. As nanocarriers, mesoporous silica nanoparticles (MSN) are utilized, whose surface is functionalized with two types of ligands, (2-thienylmethyl)hydrazine hydrochloride (H1) and (5,6-dimethylthieno[2,3-d]pyrimidin-4-yl)hydrazine (H2), which were attached to MSN through a pH-responsive hydrazone linkage. Further coordination to ruthenium(II) center yielded two types of nanomaterials MSN-H1[Ru] and MSN-H2[Ru]. Spectrophotometric measurements of the drug release kinetics at different pH (5.0, 6.0 and 7.4) confirm the enhanced release of Ru(II) complexes at lower pH values, which is further supported by inductively coupled plasma optical emission spectrometry (ICP-OES) measurements. Furthermore, the cytotoxicity effect of the released metallotherapeutics is evaluated in vitro on metastatic B16F1 melanoma cells and enhanced cancer cell-killing efficacy is demonstrated upon exposure of the nanomaterials to weakly acidic conditions. The obtained results showcase the promising capabilities of the designed MSN nanocarriers for the pH-responsive delivery of metallotherapeutics and targeted treatment of cancer.",
publisher = "MDPI",
journal = "Pharmaceutics",
title = "Ph-responsive release of ruthenium metallotherapeutics from mesoporous silica-based nanocarriers",
volume = "13",
number = "4",
pages = "460",
doi = "10.3390/pharmaceutics13040460"
}

Mladenović, M., Morgan, I., Ilić, N., Saoud, M., Pergal, M., Kaluđerović, G. N.,& Knežević, N. Ž.. (2021). Ph-responsive release of ruthenium metallotherapeutics from mesoporous silica-based nanocarriers. in Pharmaceutics
MDPI., 13(4), 460.
https://doi.org/10.3390/pharmaceutics13040460

Mladenović M, Morgan I, Ilić N, Saoud M, Pergal M, Kaluđerović GN, Knežević NŽ. Ph-responsive release of ruthenium metallotherapeutics from mesoporous silica-based nanocarriers. in Pharmaceutics. 2021;13(4):460.
doi:10.3390/pharmaceutics13040460 .

Mladenović, Minja, Morgan, Ibrahim, Ilić, Nebojša, Saoud, Mohamad, Pergal, Marija, Kaluđerović, Goran N., Knežević, Nikola Ž., "Ph-responsive release of ruthenium metallotherapeutics from mesoporous silica-based nanocarriers" in Pharmaceutics, 13, no. 4 (2021):460,
https://doi.org/10.3390/pharmaceutics13040460 . .

TY  - DATA
AU  - Mladenović, Minja
AU  - Morgan, Ibrahim
AU  - Ilić, Nebojša
AU  - Saoud, Mohamad
AU  - Pergal, Marija
AU  - Kaluđerović, Goran N.
AU  - Knežević, Nikola Ž.
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4573
AB  - Figure S1. Full range FTIR spectra of the synthesized materials. Figure S2. Particle size distribution of Ru-modified nanoparticles in water (top) and culture medium (bottom). Figure S3. EDS chromatograms of (a) MSN-H1[Ru]; (b) MSN-H2[Ru] and (c) MSN[Ru] with insets representing Ru mapping. Figure S4. UV/VIS spectra of supernatants at different pH values after 48 h of stirring. Figure S5. Viability of the B16F1 cells determined with CV and MTT assays treated (48 h) with pristine MSN on pH 5.0 and 7.2.
PB  - MDPI
T2  - Pharmaceutics
T1  - Supplementary material to: "Ph-responsive release of ruthenium metallotherapeutics from mesoporous silica-based nanocarriers"
UR  - https://hdl.handle.net/21.15107/rcub_cer_4573
ER  - 

@misc{
author = "Mladenović, Minja and Morgan, Ibrahim and Ilić, Nebojša and Saoud, Mohamad and Pergal, Marija and Kaluđerović, Goran N. and Knežević, Nikola Ž.",
year = "2021",
abstract = "Figure S1. Full range FTIR spectra of the synthesized materials. Figure S2. Particle size distribution of Ru-modified nanoparticles in water (top) and culture medium (bottom). Figure S3. EDS chromatograms of (a) MSN-H1[Ru]; (b) MSN-H2[Ru] and (c) MSN[Ru] with insets representing Ru mapping. Figure S4. UV/VIS spectra of supernatants at different pH values after 48 h of stirring. Figure S5. Viability of the B16F1 cells determined with CV and MTT assays treated (48 h) with pristine MSN on pH 5.0 and 7.2.",
publisher = "MDPI",
journal = "Pharmaceutics",
title = "Supplementary material to: "Ph-responsive release of ruthenium metallotherapeutics from mesoporous silica-based nanocarriers"",
url = "https://hdl.handle.net/21.15107/rcub_cer_4573"
}

Mladenović, M., Morgan, I., Ilić, N., Saoud, M., Pergal, M., Kaluđerović, G. N.,& Knežević, N. Ž.. (2021). Supplementary material to: "Ph-responsive release of ruthenium metallotherapeutics from mesoporous silica-based nanocarriers". in Pharmaceutics
MDPI..
https://hdl.handle.net/21.15107/rcub_cer_4573

Mladenović M, Morgan I, Ilić N, Saoud M, Pergal M, Kaluđerović GN, Knežević NŽ. Supplementary material to: "Ph-responsive release of ruthenium metallotherapeutics from mesoporous silica-based nanocarriers". in Pharmaceutics. 2021;.
https://hdl.handle.net/21.15107/rcub_cer_4573 .

Mladenović, Minja, Morgan, Ibrahim, Ilić, Nebojša, Saoud, Mohamad, Pergal, Marija, Kaluđerović, Goran N., Knežević, Nikola Ž., "Supplementary material to: "Ph-responsive release of ruthenium metallotherapeutics from mesoporous silica-based nanocarriers"" in Pharmaceutics (2021),
https://hdl.handle.net/21.15107/rcub_cer_4573 .