TY  - JOUR
AU  - Niketić, Vesna
AU  - Stojanović, Srđan
AU  - Nikolić, Aleksandra
AU  - Spasić, Mihajlo
AU  - Michelson, A.M.
PY  - 1999
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6508
AB  - The effect of NO treatment in vitro on structural and functional alterations of Cu/Zn, Mn, and Fe type of
SODs was studied. Significant difference in response to NO of Cu/ZnSOD compared to the Mn and Fe types was
demonstrated. Cu/ZnSOD was shown to be stable with respect to NO: even on prolonged exposure, NO produced
negligible effect on its structure and activity. In contrast, both Mn and Fe types were found to be NO-sensitive: exposure
to NO led to their fast and extensive inactivation, which was accompanied by extensive structural alterations, including
(in some of the samples tested) the cleavage of enzyme polypeptide chains, presumably at His residues of the enzyme
metal binding sites. The generation of nitrosonium (NO1) and nitroxyl (NO2) ions in NO treated Mn and FeSODs,
which produce enzyme modifications and inactivation, was demonstrated. The physiological and biomedical significance
of described findings is briefly discussed.
PB  - Elsevier
T2  - Free Radical Biology and Medicine
T1  - Exposure of Mn and FeSODs, but not Cu/ZnSOD, to NO leads to nitrosonium and nitroxyl ions generation which cause enzyme modification and inactivation: an in vitro study
VL  - 27
IS  - 9-10
SP  - 992
EP  - 996
DO  - 10.1016/S0891-5849(98)00256-1
ER  - 

@article{
author = "Niketić, Vesna and Stojanović, Srđan and Nikolić, Aleksandra and Spasić, Mihajlo and Michelson, A.M.",
year = "1999",
abstract = "The effect of NO treatment in vitro on structural and functional alterations of Cu/Zn, Mn, and Fe type of
SODs was studied. Significant difference in response to NO of Cu/ZnSOD compared to the Mn and Fe types was
demonstrated. Cu/ZnSOD was shown to be stable with respect to NO: even on prolonged exposure, NO produced
negligible effect on its structure and activity. In contrast, both Mn and Fe types were found to be NO-sensitive: exposure
to NO led to their fast and extensive inactivation, which was accompanied by extensive structural alterations, including
(in some of the samples tested) the cleavage of enzyme polypeptide chains, presumably at His residues of the enzyme
metal binding sites. The generation of nitrosonium (NO1) and nitroxyl (NO2) ions in NO treated Mn and FeSODs,
which produce enzyme modifications and inactivation, was demonstrated. The physiological and biomedical significance
of described findings is briefly discussed.",
publisher = "Elsevier",
journal = "Free Radical Biology and Medicine",
title = "Exposure of Mn and FeSODs, but not Cu/ZnSOD, to NO leads to nitrosonium and nitroxyl ions generation which cause enzyme modification and inactivation: an in vitro study",
volume = "27",
number = "9-10",
pages = "992-996",
doi = "10.1016/S0891-5849(98)00256-1"
}

Niketić, V., Stojanović, S., Nikolić, A., Spasić, M.,& Michelson, A.M.. (1999). Exposure of Mn and FeSODs, but not Cu/ZnSOD, to NO leads to nitrosonium and nitroxyl ions generation which cause enzyme modification and inactivation: an in vitro study. in Free Radical Biology and Medicine
Elsevier., 27(9-10), 992-996.
https://doi.org/10.1016/S0891-5849(98)00256-1

Niketić V, Stojanović S, Nikolić A, Spasić M, Michelson A. Exposure of Mn and FeSODs, but not Cu/ZnSOD, to NO leads to nitrosonium and nitroxyl ions generation which cause enzyme modification and inactivation: an in vitro study. in Free Radical Biology and Medicine. 1999;27(9-10):992-996.
doi:10.1016/S0891-5849(98)00256-1 .

Niketić, Vesna, Stojanović, Srđan, Nikolić, Aleksandra, Spasić, Mihajlo, Michelson, A.M., "Exposure of Mn and FeSODs, but not Cu/ZnSOD, to NO leads to nitrosonium and nitroxyl ions generation which cause enzyme modification and inactivation: an in vitro study" in Free Radical Biology and Medicine, 27, no. 9-10 (1999):992-996,
https://doi.org/10.1016/S0891-5849(98)00256-1 . .