TY  - JOUR
AU  - Miljuš, Goran
AU  - Penezić, Ana
AU  - Pažitná, Lucia
AU  - Gligorijević, Nikola
AU  - Baralić, Marko
AU  - Vilotić, Aleksandra
AU  - Šunderić, Miloš
AU  - Robajac, Dragana
AU  - Dobrijević, Zorana
AU  - Katrlík, Jaroslav
AU  - Nedić, Olgica
PY  - 2024
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7597
AB  - Chronic kidney disease (CKD) is a global health concern affecting approximately one billion individuals worldwide. End-stage kidney disease (ESKD), the most severe form of CKD, is
often accompanied by anemia. Peritoneal dialysis (PD), a common treatment for ESKD, utilizes the
peritoneum for solute transfer but is associated with complications including protein loss, including
transferrin (Tf) a key protein involved in iron transport. This study investigated Tf characteristics in
ESKD patients compared to healthy individuals using lectin microarray, spectroscopic techniques and immunocytochemical analysis to assess Tf interaction with transferrin receptors (TfRs). ESKD patients exhibited altered Tf glycosylation patterns, evidenced by significant changes in lectin reactivity compared to healthy controls. However, structural analyses revealed no significant differences in the Tf secondary or tertiary structures between the two groups. A functional analysis demonstrated comparable Tf-TfR interaction in both PD and healthy samples. Despite significant alterations in Tf glycosylation, structural integrity and Tf-TfR interaction remained preserved in PD patients. These findings suggest that while glycosylation changes may influence iron metabolism, they do not impair Tf function. The study highlights the importance of a glucose-free dialysis solutions in managing anemia exacerbation in PD patients with poorly controlled anemia, potentially offering a targeted therapeutic approach to improve patient outcomes.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - International Journal of Molecular Sciences
T1  - Glycosylation and Characterization of Human Transferrin in an End-Stage Kidney Disease
VL  - 25
IS  - 9
SP  - 4625
DO  - 10.3390/ijms25094625
ER  - 

@article{
author = "Miljuš, Goran and Penezić, Ana and Pažitná, Lucia and Gligorijević, Nikola and Baralić, Marko and Vilotić, Aleksandra and Šunderić, Miloš and Robajac, Dragana and Dobrijević, Zorana and Katrlík, Jaroslav and Nedić, Olgica",
year = "2024",
abstract = "Chronic kidney disease (CKD) is a global health concern affecting approximately one billion individuals worldwide. End-stage kidney disease (ESKD), the most severe form of CKD, is
often accompanied by anemia. Peritoneal dialysis (PD), a common treatment for ESKD, utilizes the
peritoneum for solute transfer but is associated with complications including protein loss, including
transferrin (Tf) a key protein involved in iron transport. This study investigated Tf characteristics in
ESKD patients compared to healthy individuals using lectin microarray, spectroscopic techniques and immunocytochemical analysis to assess Tf interaction with transferrin receptors (TfRs). ESKD patients exhibited altered Tf glycosylation patterns, evidenced by significant changes in lectin reactivity compared to healthy controls. However, structural analyses revealed no significant differences in the Tf secondary or tertiary structures between the two groups. A functional analysis demonstrated comparable Tf-TfR interaction in both PD and healthy samples. Despite significant alterations in Tf glycosylation, structural integrity and Tf-TfR interaction remained preserved in PD patients. These findings suggest that while glycosylation changes may influence iron metabolism, they do not impair Tf function. The study highlights the importance of a glucose-free dialysis solutions in managing anemia exacerbation in PD patients with poorly controlled anemia, potentially offering a targeted therapeutic approach to improve patient outcomes.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "International Journal of Molecular Sciences",
title = "Glycosylation and Characterization of Human Transferrin in an End-Stage Kidney Disease",
volume = "25",
number = "9",
pages = "4625",
doi = "10.3390/ijms25094625"
}

Miljuš, G., Penezić, A., Pažitná, L., Gligorijević, N., Baralić, M., Vilotić, A., Šunderić, M., Robajac, D., Dobrijević, Z., Katrlík, J.,& Nedić, O.. (2024). Glycosylation and Characterization of Human Transferrin in an End-Stage Kidney Disease. in International Journal of Molecular Sciences
Multidisciplinary Digital Publishing Institute (MDPI)., 25(9), 4625.
https://doi.org/10.3390/ijms25094625

Miljuš G, Penezić A, Pažitná L, Gligorijević N, Baralić M, Vilotić A, Šunderić M, Robajac D, Dobrijević Z, Katrlík J, Nedić O. Glycosylation and Characterization of Human Transferrin in an End-Stage Kidney Disease. in International Journal of Molecular Sciences. 2024;25(9):4625.
doi:10.3390/ijms25094625 .

Miljuš, Goran, Penezić, Ana, Pažitná, Lucia, Gligorijević, Nikola, Baralić, Marko, Vilotić, Aleksandra, Šunderić, Miloš, Robajac, Dragana, Dobrijević, Zorana, Katrlík, Jaroslav, Nedić, Olgica, "Glycosylation and Characterization of Human Transferrin in an End-Stage Kidney Disease" in International Journal of Molecular Sciences, 25, no. 9 (2024):4625,
https://doi.org/10.3390/ijms25094625 . .