TY  - JOUR
AU  - Zdunić, Gordana
AU  - Gođevac, Dejan
AU  - Savikin, Katarina
AU  - Novaković, Miroslav
AU  - Milosavljević, Slobodan
AU  - Petrovic, Silvana
PY  - 2011
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/928
AB  - The chemical composition and free radical scavenging properties of the MeOH extract of Hypericum richeri and its subsequent CHCl3, n-BuOH, EtOAc and aqueous extracts were investigated. Four quercetin-3-O-glycosides, three myricetin-3-O-glycosides, 3-O- and 5-O-caffeoylquinic acid, quercetin, I3,II8-biapigenin, pseudohypericin and hypericin were identified. The constituents of the extracts were quantified using HPLC-DAD (high-performance liquid chromatography-diode array detector). Isolation of the compounds from n-BuOH and EtOAc extracts was conducted by column chromatography. Antioxidant capacity of the extracts was evaluated by superoxide radical and ABTS root+ scavenging assays and inhibition of lipid peroxidation. The EtOAc extract of H. richeri was the most potent antioxidant in all experiments. Free radical scavenging compounds were identified by HPLC-DPPH root (high-performance liquid chromatography-2,2-diphenyl-1-picrylhydrazyl) post-column derivatisation method, where all phenolic compounds except I3,II8-biapigenin exhibited radical scavenging properties. This is the first report of the presence of myricetin-3-O-rutinoside, myricetin-3-O-galactoside, myricetin-3-O-glucoside, 5-O-caffeoylquinic acid and I3,II8-biapigenin in H. richeri.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Natural Product Research
T1  - Isolation and identification of phenolic compounds from Hypericum richeri Vill. and their antioxidant capacity
VL  - 25
IS  - 3
SP  - 175
EP  - 187
DO  - 10.1080/14786410802401390
ER  - 

@article{
author = "Zdunić, Gordana and Gođevac, Dejan and Savikin, Katarina and Novaković, Miroslav and Milosavljević, Slobodan and Petrovic, Silvana",
year = "2011",
abstract = "The chemical composition and free radical scavenging properties of the MeOH extract of Hypericum richeri and its subsequent CHCl3, n-BuOH, EtOAc and aqueous extracts were investigated. Four quercetin-3-O-glycosides, three myricetin-3-O-glycosides, 3-O- and 5-O-caffeoylquinic acid, quercetin, I3,II8-biapigenin, pseudohypericin and hypericin were identified. The constituents of the extracts were quantified using HPLC-DAD (high-performance liquid chromatography-diode array detector). Isolation of the compounds from n-BuOH and EtOAc extracts was conducted by column chromatography. Antioxidant capacity of the extracts was evaluated by superoxide radical and ABTS root+ scavenging assays and inhibition of lipid peroxidation. The EtOAc extract of H. richeri was the most potent antioxidant in all experiments. Free radical scavenging compounds were identified by HPLC-DPPH root (high-performance liquid chromatography-2,2-diphenyl-1-picrylhydrazyl) post-column derivatisation method, where all phenolic compounds except I3,II8-biapigenin exhibited radical scavenging properties. This is the first report of the presence of myricetin-3-O-rutinoside, myricetin-3-O-galactoside, myricetin-3-O-glucoside, 5-O-caffeoylquinic acid and I3,II8-biapigenin in H. richeri.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Natural Product Research",
title = "Isolation and identification of phenolic compounds from Hypericum richeri Vill. and their antioxidant capacity",
volume = "25",
number = "3",
pages = "175-187",
doi = "10.1080/14786410802401390"
}

Zdunić, G., Gođevac, D., Savikin, K., Novaković, M., Milosavljević, S.,& Petrovic, S.. (2011). Isolation and identification of phenolic compounds from Hypericum richeri Vill. and their antioxidant capacity. in Natural Product Research
Taylor & Francis Ltd, Abingdon., 25(3), 175-187.
https://doi.org/10.1080/14786410802401390

Zdunić G, Gođevac D, Savikin K, Novaković M, Milosavljević S, Petrovic S. Isolation and identification of phenolic compounds from Hypericum richeri Vill. and their antioxidant capacity. in Natural Product Research. 2011;25(3):175-187.
doi:10.1080/14786410802401390 .

Zdunić, Gordana, Gođevac, Dejan, Savikin, Katarina, Novaković, Miroslav, Milosavljević, Slobodan, Petrovic, Silvana, "Isolation and identification of phenolic compounds from Hypericum richeri Vill. and their antioxidant capacity" in Natural Product Research, 25, no. 3 (2011):175-187,
https://doi.org/10.1080/14786410802401390 . .

TY  - JOUR
AU  - Zdunić, Gordana
AU  - Gođevac, Dejan
AU  - Milenković, Marina
AU  - Savikin, Katarina
AU  - Menković, Nebojša
AU  - Petrovic, Silvana
PY  - 2010
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/702
AB  - Oil extracts of flowering tops of Hypericum richeri Vill. prepared in three different ways were evaluated for chemical composition, and anti-inflammatory and gastroprotective activities. An HPLC method was developed for determination of two dominant flavonoids, quercetin and I3,II8-biapigenin. The carrageenan-induced rat paw edema test was used for screening the anti-inflammatory activity, while indomethacin-induced rat gastric mucosa damage test was used for evaluation of gastroprotective activity. The oil extract prepared by maceration with 96% ethanol, followed by extraction with sunflower oil by heating on a water bath, exhibited the highest anti-inflammatory (38.4 %) and gastroprotective activities (gastric damage score of 0.9). The same oil extract had the highest content of quercetin (49 mu g/mL) and I3,II8-biapigenin (60 mu g/mL). These results approve the usage of oil extracts of H. richeri as an anti-inflammatory and gastroprotective agent.
PB  - Natural Product Incorporation
T2  - Natural Product Communications
T1  - Anti-inflammatory and Gastroprotective Properties of Hypericum richeri Oil Extracts
VL  - 5
IS  - 8
SP  - 1215
EP  - 1218
UR  - https://hdl.handle.net/21.15107/rcub_cer_702
ER  - 

@article{
author = "Zdunić, Gordana and Gođevac, Dejan and Milenković, Marina and Savikin, Katarina and Menković, Nebojša and Petrovic, Silvana",
year = "2010",
abstract = "Oil extracts of flowering tops of Hypericum richeri Vill. prepared in three different ways were evaluated for chemical composition, and anti-inflammatory and gastroprotective activities. An HPLC method was developed for determination of two dominant flavonoids, quercetin and I3,II8-biapigenin. The carrageenan-induced rat paw edema test was used for screening the anti-inflammatory activity, while indomethacin-induced rat gastric mucosa damage test was used for evaluation of gastroprotective activity. The oil extract prepared by maceration with 96% ethanol, followed by extraction with sunflower oil by heating on a water bath, exhibited the highest anti-inflammatory (38.4 %) and gastroprotective activities (gastric damage score of 0.9). The same oil extract had the highest content of quercetin (49 mu g/mL) and I3,II8-biapigenin (60 mu g/mL). These results approve the usage of oil extracts of H. richeri as an anti-inflammatory and gastroprotective agent.",
publisher = "Natural Product Incorporation",
journal = "Natural Product Communications",
title = "Anti-inflammatory and Gastroprotective Properties of Hypericum richeri Oil Extracts",
volume = "5",
number = "8",
pages = "1215-1218",
url = "https://hdl.handle.net/21.15107/rcub_cer_702"
}

Zdunić, G., Gođevac, D., Milenković, M., Savikin, K., Menković, N.,& Petrovic, S.. (2010). Anti-inflammatory and Gastroprotective Properties of Hypericum richeri Oil Extracts. in Natural Product Communications
Natural Product Incorporation., 5(8), 1215-1218.
https://hdl.handle.net/21.15107/rcub_cer_702

Zdunić G, Gođevac D, Milenković M, Savikin K, Menković N, Petrovic S. Anti-inflammatory and Gastroprotective Properties of Hypericum richeri Oil Extracts. in Natural Product Communications. 2010;5(8):1215-1218.
https://hdl.handle.net/21.15107/rcub_cer_702 .

Zdunić, Gordana, Gođevac, Dejan, Milenković, Marina, Savikin, Katarina, Menković, Nebojša, Petrovic, Silvana, "Anti-inflammatory and Gastroprotective Properties of Hypericum richeri Oil Extracts" in Natural Product Communications, 5, no. 8 (2010):1215-1218,
https://hdl.handle.net/21.15107/rcub_cer_702 .

TY  - JOUR
AU  - Zdunić, Gordana
AU  - Gođevac, Dejan
AU  - Milenković, Marina
AU  - Vučićević, D.
AU  - Šavikin, Katarina
AU  - Menković, Nebojša
AU  - Petrović, S.
PY  - 2009
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/625
AB  - Oil extracts of Hypericum perforatum L. (Oleum Hyperici) were prepared in three different ways according to the prescriptions from traditional medicine. Variability of constituents and biological activity were evaluated in the obtained oil extracts. The carrageenan-induced rat paw edema test has been used for screening the antiinflammatory activity, while the indomethacin-induced rat gastric mucosa damage test was used for evaluation of gastroprotective activity. All examined oil extracts possessed antiinflammatory and gastroprotective activity. Among them, the oil extract prepared by maceration with 96% ethanol, followed by extraction with sunflower oil by heating on a water bath (extract 2), in a dose of 1.25 mL/kg p.o., exhibited the highest antiinflammatory effect (95.24 ± 11.66%) and gastroprotective activity (gastric damage score of 0.21 ± 0.12). The same oil extract had the highest content of quercetin and I3,II8-biapigenin (129 ± 9 μg/mL and 52 ± 4 μg/mL, respectively). Quercetin and I3,II8-biapigenin exhibited antiinflammatory activity similar to those of indomethacin as well as significant gastroprotective activity. The results provide evidence for the usage of Oleum Hyperici as an anti-inflammatory and gastroprotective agent, which has been based previously only on ethnopharmacological claims. Copyright
T2  - Phytotherapy Research
T1  - Evaluation of Hypericum perforatum oil extracts for an antiinflammatory and gastroprotective activity in rats
VL  - 23
IS  - 11
SP  - 1559
EP  - 1564
DO  - 10.1002/ptr.2809
ER  - 

@article{
author = "Zdunić, Gordana and Gođevac, Dejan and Milenković, Marina and Vučićević, D. and Šavikin, Katarina and Menković, Nebojša and Petrović, S.",
year = "2009",
abstract = "Oil extracts of Hypericum perforatum L. (Oleum Hyperici) were prepared in three different ways according to the prescriptions from traditional medicine. Variability of constituents and biological activity were evaluated in the obtained oil extracts. The carrageenan-induced rat paw edema test has been used for screening the antiinflammatory activity, while the indomethacin-induced rat gastric mucosa damage test was used for evaluation of gastroprotective activity. All examined oil extracts possessed antiinflammatory and gastroprotective activity. Among them, the oil extract prepared by maceration with 96% ethanol, followed by extraction with sunflower oil by heating on a water bath (extract 2), in a dose of 1.25 mL/kg p.o., exhibited the highest antiinflammatory effect (95.24 ± 11.66%) and gastroprotective activity (gastric damage score of 0.21 ± 0.12). The same oil extract had the highest content of quercetin and I3,II8-biapigenin (129 ± 9 μg/mL and 52 ± 4 μg/mL, respectively). Quercetin and I3,II8-biapigenin exhibited antiinflammatory activity similar to those of indomethacin as well as significant gastroprotective activity. The results provide evidence for the usage of Oleum Hyperici as an anti-inflammatory and gastroprotective agent, which has been based previously only on ethnopharmacological claims. Copyright",
journal = "Phytotherapy Research",
title = "Evaluation of Hypericum perforatum oil extracts for an antiinflammatory and gastroprotective activity in rats",
volume = "23",
number = "11",
pages = "1559-1564",
doi = "10.1002/ptr.2809"
}

Zdunić, G., Gođevac, D., Milenković, M., Vučićević, D., Šavikin, K., Menković, N.,& Petrović, S.. (2009). Evaluation of Hypericum perforatum oil extracts for an antiinflammatory and gastroprotective activity in rats. in Phytotherapy Research, 23(11), 1559-1564.
https://doi.org/10.1002/ptr.2809

Zdunić G, Gođevac D, Milenković M, Vučićević D, Šavikin K, Menković N, Petrović S. Evaluation of Hypericum perforatum oil extracts for an antiinflammatory and gastroprotective activity in rats. in Phytotherapy Research. 2009;23(11):1559-1564.
doi:10.1002/ptr.2809 .

Zdunić, Gordana, Gođevac, Dejan, Milenković, Marina, Vučićević, D., Šavikin, Katarina, Menković, Nebojša, Petrović, S., "Evaluation of Hypericum perforatum oil extracts for an antiinflammatory and gastroprotective activity in rats" in Phytotherapy Research, 23, no. 11 (2009):1559-1564,
https://doi.org/10.1002/ptr.2809 . .

TY  - JOUR
AU  - Rakić, S.
AU  - Petrović, S.
AU  - Kukić, J.
AU  - Jadranin, Milka
AU  - Tešević, Vele
AU  - Povrenović, D.
AU  - Šiler-Marinković, S.
PY  - 2007
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/362
AB  - The aim of the present work was to investigate and compare phenolic compounds and antioxidant activity of methanol extracts of Quercus robur and Quercus cerris acorn kernels obtained before and after thermal treatment. Content of total phenolics, tannins, non-tannin phenolics and flavonoids was determined spectrophotometrically and content of gallic acid with HPLC. Antioxidant activity of the samples was assayed through FRAP (Ferric Reducing Antioxidant Power), DPPH scavenging test and inhibition of Fe2+/ascorbate induced lipid peroxidation. Extracts of native and thermally treated kernels showed high antioxidant activity, with extracts of thermally treated kernels being more active than extracts of native ones. Hydrolysable tannins and gallic acid were identified in all samples. Non-tannin phenolics, including gallic acid, were present in significantly higher quantities in thermally treated samples, whilst tannin content decreased. This indicates that during thermal treatment hydrolysable tannins were degraded. As the result of this degradation and consequent increase of non-tannin phenolics content, and amongst them especially gallic acid, thermally treated samples possess higher antioxidant activity than do the native ones. The obtained results have provided further grounds for establishing Q. robur and Q. cerris acorn kernels as a source for functional food preparation.
PB  - Elsevier
T2  - Food Chemistry
T1  - Influence of thermal treatment on phenolic compounds and antioxidant properties of oak acorns from Serbia
VL  - 104
IS  - 2
SP  - 830
EP  - 834
DO  - 10.1016/j.foodchem.2007.01.025
ER  - 

@article{
author = "Rakić, S. and Petrović, S. and Kukić, J. and Jadranin, Milka and Tešević, Vele and Povrenović, D. and Šiler-Marinković, S.",
year = "2007",
abstract = "The aim of the present work was to investigate and compare phenolic compounds and antioxidant activity of methanol extracts of Quercus robur and Quercus cerris acorn kernels obtained before and after thermal treatment. Content of total phenolics, tannins, non-tannin phenolics and flavonoids was determined spectrophotometrically and content of gallic acid with HPLC. Antioxidant activity of the samples was assayed through FRAP (Ferric Reducing Antioxidant Power), DPPH scavenging test and inhibition of Fe2+/ascorbate induced lipid peroxidation. Extracts of native and thermally treated kernels showed high antioxidant activity, with extracts of thermally treated kernels being more active than extracts of native ones. Hydrolysable tannins and gallic acid were identified in all samples. Non-tannin phenolics, including gallic acid, were present in significantly higher quantities in thermally treated samples, whilst tannin content decreased. This indicates that during thermal treatment hydrolysable tannins were degraded. As the result of this degradation and consequent increase of non-tannin phenolics content, and amongst them especially gallic acid, thermally treated samples possess higher antioxidant activity than do the native ones. The obtained results have provided further grounds for establishing Q. robur and Q. cerris acorn kernels as a source for functional food preparation.",
publisher = "Elsevier",
journal = "Food Chemistry",
title = "Influence of thermal treatment on phenolic compounds and antioxidant properties of oak acorns from Serbia",
volume = "104",
number = "2",
pages = "830-834",
doi = "10.1016/j.foodchem.2007.01.025"
}

Rakić, S., Petrović, S., Kukić, J., Jadranin, M., Tešević, V., Povrenović, D.,& Šiler-Marinković, S.. (2007). Influence of thermal treatment on phenolic compounds and antioxidant properties of oak acorns from Serbia. in Food Chemistry
Elsevier., 104(2), 830-834.
https://doi.org/10.1016/j.foodchem.2007.01.025

Rakić S, Petrović S, Kukić J, Jadranin M, Tešević V, Povrenović D, Šiler-Marinković S. Influence of thermal treatment on phenolic compounds and antioxidant properties of oak acorns from Serbia. in Food Chemistry. 2007;104(2):830-834.
doi:10.1016/j.foodchem.2007.01.025 .

Rakić, S., Petrović, S., Kukić, J., Jadranin, Milka, Tešević, Vele, Povrenović, D., Šiler-Marinković, S., "Influence of thermal treatment on phenolic compounds and antioxidant properties of oak acorns from Serbia" in Food Chemistry, 104, no. 2 (2007):830-834,
https://doi.org/10.1016/j.foodchem.2007.01.025 . .