@conference{
author = "Krizmanić, Jelena and Vidaković, Danijela and Stupar, Miloš and Ljaljević Grbić, Milica and Krizmanić, Imre and Stamenković, Srđan and Breka, Katarina",
year = "2017",
abstract = "It is known that diatoms may be present on the skin of various animals (whales, dolphins, manatees, turtles…) (Frankovich et al. 2015, Wetzel et al. 2012). In our study diatoms were collected from the skin of two green frogs’ species: Pelophylax esculentus and P. ridibundus. Studied frogs were captured from the Special Nature Reserve “Deliblatska Pescara” at the locality Stevanove ravnice (Vojvodina province, Serbia). Two sampling methods were performed: (1) nonaggressive adhesive tape method (Urzì and de Leo 2001) and (2) scraping by toothbrush. In laboratory conditions, samples taken by the first method were stained with a drop of Lactophenol Cotton Blue and put on slides to be analyzed. Samples taken by the second method were treated by hot HCl and KMnO4 (Taylor et al. 2005) in order to obtain permanent slides. Light microscope observations and micrographs were made by Zeiss AxioImagerM.1 microscope with DIC optics (x1000 and x1600 magnification) and AxioVision 4.8 software.
The present study describes diversity of diatoms from the green frog skin. According to literature data no studies have been published on diatoms living on the frog skin.
Analysis of adhesive tape samples confirmed the presence of live diatom cells on the frog skin. Among them the most abundant were taxa from the genera Cocconeis, Epithemia, Gomphonema, Navicula and Rhopalodia. Presence of c. 30 diatom genera, such as Amphora, Craticula, Cymbella, Cymbopleura, Cymatopleura, Diploneis, Encyonema, Epithemia, Fallacia, Gomphonema, Luticola, Navicula, Neidium, Nitzschia, Pinnularia, Planothidium, Rhopalodia, Staurosira, Surirella, etc., was observed on permanent slides. The most dominant taxa were: Geissleria decussis sensu lato, Placoneis sp., Lemnicola hungarica, Cocconeis placentula var. lineate and Sellaphora bacillum.
We consider that the great diversity and abundance of diatoms is consequence of their transfer from the environment (mud and macrophytes from eutrophic ponds) onto mucous frog skin. Highly mucous skin is an excellent environment which enables primary conditions for the survival of diatoms. Future research, especially under laboratory conditions, could show whether the frog skin is permanent or temporary diatom habitat.",
publisher = "Prague : Charles University in Prague",
journal = "Programme and abstract book - 11th Central European Diatom Meeting",
title = "Diatoms on the green frogs skin (Pelophylax esculentus and P. ridibundus)",
pages = "114-114",
url = "https://hdl.handle.net/21.15107/rcub_cer_4646"
}
