@conference{
author = "Aćimović, Jelena and Gligorijević, Nikola and Radomirović, Mirjana and Vasović, Tamara and Stojadinović, Marija and Stanić-Vučinić, Dragana and Ćirković-Veličković, Tanja",
year = "2024",
abstract = "Microplastics (MPs) interact with proteins, forming a complex called a protein corona. This corona
alters the biological identity and properties of MPs, enabling them to evade the immune system,
persist longer in circulation, and disrupt cellular and molecular processes. Biofilm formation on
MPs occurs in several steps, starting with initial binding of molecules to form a hard corona,
followed by the formation of a soft corona. β-Lactoglobulin (BLG), a major whey protein in bovine
milk, is highly valued as a food ingredient but poses significant health risks to milk-allergic
individuals. This study aimed to understand BLG's interactions with selected MPs (polyethylene
terephthalate, PET, <80μm; polypropylene, PP, 63-180μm). BLG, isolated from
unpasteurized/pasteurized milk, was incubated with PP and PET in simulated physiological fluids.
The resulting coronas were analyzed using reduced SDS-PAGE electrophoresis and densitometry.
BLG exhibited low binding to both MPs, with pasteurized BLG showing the lowest binding to PP
(less than 1% of control in its soft corona). There were differences in the protein content of hard
coronas between pasteurized and unpasteurized BLG, with a slightly higher percentage of bound
proteins in the hard corona of unpasteurized BLG. Additionally, the protein profile of hard coronas
differed between PET and PP incubated with pasteurized BLG compared to those with
unpasteurized BLG. These findings suggest that the presence of MPs may affect the bioavailability
and allergenic properties of both pasteurized and unpasteurized BLG., Interakcija mikroplastike (MP) s proteinima stvara kompleks poznat kao proteinska korona. U okviru korone, MP dobija nove biološke osobine, uključujući izbegavanje imunog sistema, dugotrajnije zadržavanje u cirkulaciji i ometanje ćelijskih procesa. Formiranje biofilma na površini MP je višestepeni proces, gde se nakon prvobitnog vezivanja molekula s visokim afinitetom i formiranja čvrste korone, može razviti i labavija korona u kasnijim fazama. β-laktoglobulin (BLG), glavni protein u surutki kravljeg mleka, često korišćen u hrani, može izazvati značajne zdravstvene probleme kod osoba alergičnih na mleko. Ovo istraživanje je imalo za cilj da pruži bolji uvid u interakcije između BLG kao alergena hrane i odabranih MP (polietilen-tereftalat, PET, <80μm; polipropilen, PP, 63-180μm). Izolovani nepasterizovani/pasterizovani BLG je inkubiran s PP i PET in vitro, u simuliranim fiziološkim tečnostima, a formirane korone su analizirane SDSPAGE elektroforezom pod redukujućim uslovima i denzitometrijom. Rezultati pokazuju da BLG slabo vezuje za obe MP. Postoji razlika u proteinima čvrstih korona između pasterizovanog i nepasterizovanog BLG, kao i u proteinskom profilu korona PP i PET inkubiranih s pasterizovanim ili nepasterizovanim BLG. Naši rezultati ukazuju da prisustvo MP može uticati na biodostupnost i alergene osobine i pasterizovanog i nepasterizovanog BLG.",
publisher = "Serbian Chemical Society, Srpsko hemijsko društvo",
journal = "60. Savetovanje Srpskog hemijskog društva - Kratki izvodi radova, Niš 8. i 9. jun 2024. godine",
title = "Soft i hard korona mikroplastike u interakciji sa alergenom β-laktoglobulinom kravljeg mleka, Soft and hard corona of microplastics interacted with allergenic bovine milk β-lactoglobulin",
pages = "53-53",
url = "https://hdl.handle.net/21.15107/rcub_cer_7649"
}
