TY  - CONF
AU  - Stupar, Sofija
AU  - Tešević, Vele
AU  - Stanković, Jovana
AU  - Ćosić, Tatjana
AU  - Devrnja, Nina
AU  - Tubić, Ljiljana
AU  - Cingel, Aleksandar
AU  - Ninković, Slavica
AU  - Vinterhalter, Branka
AU  - Savić, Jelena
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7514
AB  - When attacked, plants activate defense mechanisms including volatile emission. Besides havinga pest repellent effect, volatiles can induce defense response in surrounding unharmed plants.French marigold (Tagetes patula L.) essential oil (EO) was isolated from the above-ground partsof plants collected from nature by hydrodistillation using Clevenger apparatus. GC/MS analysisrevealed the presence of 42 compounds, of which 97% (32 compounds) have been identified.The monoterpene fraction was dominant (76.9%) with terpinolene (32.4%) and limonene (14.7%)as the most abundant compounds. In order to investigate the potential of marigold EO to inducethe defense of potato, we analyzed 3 genes related to the induced resistance (IR): pathogenesisrelated PR-2 and PR-5 genes, and proteinase inhibitor Pin2. The potato plants were exposed to EOvolatiles during 4, 8 and 12 h. Two leaves of EO-treated or untreated control plants were collected,and plants were then additionally injured by mechanically wounding leaves across the leaf surface.After 24 h of wounding, another two leaves from each plant were collected and expressionof the IR related genes was analyzed by qPCR method. EO induced altered expression of PR genes,while Pin2 wasn’t affected by the treatment. Mechanical wounding increased the expression ofall genes after 4 and 8 h of EO treatment, with up to 4-fold change in PR-2. However, in plantsexposed to EO for 12 h before wounding, the level of transcription in PR-2 and Pin2 was similar tothat detected in untreated controls. These findings support the efforts for environmentally-safepest control in crops by using natural compounds.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts
T1  - Induction of potato (Solanum tuberosum L.) defense genes by French marigold (Tagetes patula L.) essential oil
SP  - 58
EP  - 58
UR  - https://hdl.handle.net/21.15107/rcub_cer_7514
ER  - 

@conference{
author = "Stupar, Sofija and Tešević, Vele and Stanković, Jovana and Ćosić, Tatjana and Devrnja, Nina and Tubić, Ljiljana and Cingel, Aleksandar and Ninković, Slavica and Vinterhalter, Branka and Savić, Jelena",
year = "2018",
abstract = "When attacked, plants activate defense mechanisms including volatile emission. Besides havinga pest repellent effect, volatiles can induce defense response in surrounding unharmed plants.French marigold (Tagetes patula L.) essential oil (EO) was isolated from the above-ground partsof plants collected from nature by hydrodistillation using Clevenger apparatus. GC/MS analysisrevealed the presence of 42 compounds, of which 97% (32 compounds) have been identified.The monoterpene fraction was dominant (76.9%) with terpinolene (32.4%) and limonene (14.7%)as the most abundant compounds. In order to investigate the potential of marigold EO to inducethe defense of potato, we analyzed 3 genes related to the induced resistance (IR): pathogenesisrelated PR-2 and PR-5 genes, and proteinase inhibitor Pin2. The potato plants were exposed to EOvolatiles during 4, 8 and 12 h. Two leaves of EO-treated or untreated control plants were collected,and plants were then additionally injured by mechanically wounding leaves across the leaf surface.After 24 h of wounding, another two leaves from each plant were collected and expressionof the IR related genes was analyzed by qPCR method. EO induced altered expression of PR genes,while Pin2 wasn’t affected by the treatment. Mechanical wounding increased the expression ofall genes after 4 and 8 h of EO treatment, with up to 4-fold change in PR-2. However, in plantsexposed to EO for 12 h before wounding, the level of transcription in PR-2 and Pin2 was similar tothat detected in untreated controls. These findings support the efforts for environmentally-safepest control in crops by using natural compounds.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts",
title = "Induction of potato (Solanum tuberosum L.) defense genes by French marigold (Tagetes patula L.) essential oil",
pages = "58-58",
url = "https://hdl.handle.net/21.15107/rcub_cer_7514"
}

Stupar, S., Tešević, V., Stanković, J., Ćosić, T., Devrnja, N., Tubić, L., Cingel, A., Ninković, S., Vinterhalter, B.,& Savić, J.. (2018). Induction of potato (Solanum tuberosum L.) defense genes by French marigold (Tagetes patula L.) essential oil. in 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts
Belgrade: Serbian Plant Physiology Society., 58-58.
https://hdl.handle.net/21.15107/rcub_cer_7514

Stupar S, Tešević V, Stanković J, Ćosić T, Devrnja N, Tubić L, Cingel A, Ninković S, Vinterhalter B, Savić J. Induction of potato (Solanum tuberosum L.) defense genes by French marigold (Tagetes patula L.) essential oil. in 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts. 2018;:58-58.
https://hdl.handle.net/21.15107/rcub_cer_7514 .

Stupar, Sofija, Tešević, Vele, Stanković, Jovana, Ćosić, Tatjana, Devrnja, Nina, Tubić, Ljiljana, Cingel, Aleksandar, Ninković, Slavica, Vinterhalter, Branka, Savić, Jelena, "Induction of potato (Solanum tuberosum L.) defense genes by French marigold (Tagetes patula L.) essential oil" in 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts (2018):58-58,
https://hdl.handle.net/21.15107/rcub_cer_7514 .

TY  - JOUR
AU  - Radosavljevic, Jasna Simonovic
AU  - Pristov, Jelena Bogdanovic
AU  - Mitrović, Aleksandra Lj.
AU  - Steinbach, Gabor
AU  - Mouille, Gregory
AU  - Tufegdžić, Srđan
AU  - Maksimović, Vuk
AU  - Mutavdzic, Dragosav
AU  - Janosevic, Dusica
AU  - Vuković, Marina
AU  - Garab, Gyozo
AU  - Radotić, Ksenija
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2480
AB  - In the original publication of the article, one of the project numbers was omitted in the Acknowledgments. The correct version is provided below.
PB  - Springer, Dordrecht
T2  - Cellulose
T1  - Correction to: Parenchyma cell wall structure in twining stem of Dioscorea balcanica (vol 24, pg 4653, 2017)
VL  - 25
IS  - 4
SP  - 2767
EP  - 2767
DO  - 10.1007/s10570-018-1706-6
ER  - 

@article{
author = "Radosavljevic, Jasna Simonovic and Pristov, Jelena Bogdanovic and Mitrović, Aleksandra Lj. and Steinbach, Gabor and Mouille, Gregory and Tufegdžić, Srđan and Maksimović, Vuk and Mutavdzic, Dragosav and Janosevic, Dusica and Vuković, Marina and Garab, Gyozo and Radotić, Ksenija",
year = "2018",
abstract = "In the original publication of the article, one of the project numbers was omitted in the Acknowledgments. The correct version is provided below.",
publisher = "Springer, Dordrecht",
journal = "Cellulose",
title = "Correction to: Parenchyma cell wall structure in twining stem of Dioscorea balcanica (vol 24, pg 4653, 2017)",
volume = "25",
number = "4",
pages = "2767-2767",
doi = "10.1007/s10570-018-1706-6"
}

Radosavljevic, J. S., Pristov, J. B., Mitrović, A. Lj., Steinbach, G., Mouille, G., Tufegdžić, S., Maksimović, V., Mutavdzic, D., Janosevic, D., Vuković, M., Garab, G.,& Radotić, K.. (2018). Correction to: Parenchyma cell wall structure in twining stem of Dioscorea balcanica (vol 24, pg 4653, 2017). in Cellulose
Springer, Dordrecht., 25(4), 2767-2767.
https://doi.org/10.1007/s10570-018-1706-6

Radosavljevic JS, Pristov JB, Mitrović AL, Steinbach G, Mouille G, Tufegdžić S, Maksimović V, Mutavdzic D, Janosevic D, Vuković M, Garab G, Radotić K. Correction to: Parenchyma cell wall structure in twining stem of Dioscorea balcanica (vol 24, pg 4653, 2017). in Cellulose. 2018;25(4):2767-2767.
doi:10.1007/s10570-018-1706-6 .

Radosavljevic, Jasna Simonovic, Pristov, Jelena Bogdanovic, Mitrović, Aleksandra Lj., Steinbach, Gabor, Mouille, Gregory, Tufegdžić, Srđan, Maksimović, Vuk, Mutavdzic, Dragosav, Janosevic, Dusica, Vuković, Marina, Garab, Gyozo, Radotić, Ksenija, "Correction to: Parenchyma cell wall structure in twining stem of Dioscorea balcanica (vol 24, pg 4653, 2017)" in Cellulose, 25, no. 4 (2018):2767-2767,
https://doi.org/10.1007/s10570-018-1706-6 . .

TY  - JOUR
AU  - Radosavljevic, Jasna Simonovic
AU  - Pristov, Jelena Bogdanovic
AU  - Mitrović, Aleksandra Lj.
AU  - Steinbach, Gabor
AU  - Mouille, Gregory
AU  - Tufegdžić, Srđan
AU  - Maksimović, Vuk
AU  - Mutavdzic, Dragosav
AU  - Janosevic, Dusica
AU  - Vuković, Marina
AU  - Garab, Gyozo
AU  - Radotić, Ksenija
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2090
AB  - Anatomical adaptation of liana plants includes structural changes in cell walls of different tissues: fibers, vessel elements and tracheids. However, the contribution of parenchyma cells to stem twining in liana plants is mostly unknown. The aim of this investigation is to determine changes in stem parenchyma cell walls that are correlated with the twinning process in liana plants. Parenchyma cell wall structure was studied on the stem cross sections of straight and twisted internodes of monocotyledonous liana Dioscorea balcanica, by different microscopy techniques: light microscopy, scanning electron microscopy, fluorescence detected linear dichroism microscopy and Fourier transform infrared microspectrometry. In addition, chemical analysis of the entire stem internodes was performed using photometric and chromatographic methods. Parenchyma cell walls of twisted D. balcanica internodes are characterized by: lower amounts of cellulose (obtained by FTIR microspectrometry) with different cellulose microfibril orientation (shown by Scanning electron microscopy), but no changes in "cellulose fibril order" (obtained by Differential polarization laser scanning microscopy); lower amounts of xyloglucan, higher amounts of xylan, higher amounts of lignin with modified organization-less condensed lignin (obtained by FTIR microspectrometry). At the same time, chemical analysis of the entire internodes did not show significant differences in lignin content and cell wall bound phenols related to stem twining, except for the presence of diferulate cross-links exclusively in twisted internodes. Our results indicate that adaptations to mechanical strain in D. balcanica stems involve modifications in parenchyma cell wall structure and chemistry, which provide decreased stiffness, higher strength and increased elasticity of twisted internodes.
PB  - Springer, Dordrecht
T2  - Cellulose
T1  - Parenchyma cell wall structure in twining stem of Dioscorea balcanica
VL  - 24
IS  - 11
SP  - 4653
EP  - 4669
DO  - 10.1007/s10570-017-1460-1
ER  - 

@article{
author = "Radosavljevic, Jasna Simonovic and Pristov, Jelena Bogdanovic and Mitrović, Aleksandra Lj. and Steinbach, Gabor and Mouille, Gregory and Tufegdžić, Srđan and Maksimović, Vuk and Mutavdzic, Dragosav and Janosevic, Dusica and Vuković, Marina and Garab, Gyozo and Radotić, Ksenija",
year = "2017",
abstract = "Anatomical adaptation of liana plants includes structural changes in cell walls of different tissues: fibers, vessel elements and tracheids. However, the contribution of parenchyma cells to stem twining in liana plants is mostly unknown. The aim of this investigation is to determine changes in stem parenchyma cell walls that are correlated with the twinning process in liana plants. Parenchyma cell wall structure was studied on the stem cross sections of straight and twisted internodes of monocotyledonous liana Dioscorea balcanica, by different microscopy techniques: light microscopy, scanning electron microscopy, fluorescence detected linear dichroism microscopy and Fourier transform infrared microspectrometry. In addition, chemical analysis of the entire stem internodes was performed using photometric and chromatographic methods. Parenchyma cell walls of twisted D. balcanica internodes are characterized by: lower amounts of cellulose (obtained by FTIR microspectrometry) with different cellulose microfibril orientation (shown by Scanning electron microscopy), but no changes in "cellulose fibril order" (obtained by Differential polarization laser scanning microscopy); lower amounts of xyloglucan, higher amounts of xylan, higher amounts of lignin with modified organization-less condensed lignin (obtained by FTIR microspectrometry). At the same time, chemical analysis of the entire internodes did not show significant differences in lignin content and cell wall bound phenols related to stem twining, except for the presence of diferulate cross-links exclusively in twisted internodes. Our results indicate that adaptations to mechanical strain in D. balcanica stems involve modifications in parenchyma cell wall structure and chemistry, which provide decreased stiffness, higher strength and increased elasticity of twisted internodes.",
publisher = "Springer, Dordrecht",
journal = "Cellulose",
title = "Parenchyma cell wall structure in twining stem of Dioscorea balcanica",
volume = "24",
number = "11",
pages = "4653-4669",
doi = "10.1007/s10570-017-1460-1"
}

Radosavljevic, J. S., Pristov, J. B., Mitrović, A. Lj., Steinbach, G., Mouille, G., Tufegdžić, S., Maksimović, V., Mutavdzic, D., Janosevic, D., Vuković, M., Garab, G.,& Radotić, K.. (2017). Parenchyma cell wall structure in twining stem of Dioscorea balcanica. in Cellulose
Springer, Dordrecht., 24(11), 4653-4669.
https://doi.org/10.1007/s10570-017-1460-1

Radosavljevic JS, Pristov JB, Mitrović AL, Steinbach G, Mouille G, Tufegdžić S, Maksimović V, Mutavdzic D, Janosevic D, Vuković M, Garab G, Radotić K. Parenchyma cell wall structure in twining stem of Dioscorea balcanica. in Cellulose. 2017;24(11):4653-4669.
doi:10.1007/s10570-017-1460-1 .

Radosavljevic, Jasna Simonovic, Pristov, Jelena Bogdanovic, Mitrović, Aleksandra Lj., Steinbach, Gabor, Mouille, Gregory, Tufegdžić, Srđan, Maksimović, Vuk, Mutavdzic, Dragosav, Janosevic, Dusica, Vuković, Marina, Garab, Gyozo, Radotić, Ksenija, "Parenchyma cell wall structure in twining stem of Dioscorea balcanica" in Cellulose, 24, no. 11 (2017):4653-4669,
https://doi.org/10.1007/s10570-017-1460-1 . .