TY  - CONF
AU  - Mutić, Tamara
AU  - Gligorijević, Nikola
AU  - Ćirković Veličković, Tanja
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7281
AB  - Microplastics are plastic fibers, particles or films with diameters smaller than 5 mm and they have shown different
effects on proteins [1]. Microplastics (MPs) are small in size, have low densities, can exist in the atmosphere for a
long time and can easily be spread by wind [2]. The objective of this study was to investigate adsorption affinity of
different types of MPs (polyethylene terephthalate (PET), polystyrene (PS) and polyvinyl chloride (PVC)) with
ovalbumin. In this study ovalbumin, isolated from chicken egg white, was used. Plastics were mixed with ovalbumin
for 1,2,4 and 19 h and then the absorbance of the remaining protein in the solution was measured at 280nm. In
addition, Langmuir isotherm mathematical model to calculate the adsorption affinity of ovalbumin for MPs was used.
We determined affinity constants by using Langmuir isotherm models for different particle size (PS 120 μm and PS
500 μm), different types of plastics (PET, PS and PVC) and pH values (3 and 7,2). Adsorption experiment results
showed that adsorption depends on type of plastics. Our results showed that PVC did not adsorb ovalbumin,
however, PET and PS have interacted with protein. Adsorption capacities of all analysed MPs increase with pH of
solution. Under different pH values, MPs and protein have different charges that may affect adsorption
characteristics. With increase of pH from 3 to 7, the affinity for protein adsorption increased 1.4 times for PS (smaller
in size) while for PS (bigger size) and PET protein affinity was two times higher at pH 3.
PB  - Sociedade Portuguesa de Química
C3  - Book of Abstracts - XXI EuroFoodChem conference, 22nd-24th November, 2021, Virtual Congress
T1  - Binding affinity ovalbumin on different type of microplastics using Langmuir isotherm
SP  - 178
EP  - 178
UR  - https://hdl.handle.net/21.15107/rcub_cer_7281
ER  - 

@conference{
author = "Mutić, Tamara and Gligorijević, Nikola and Ćirković Veličković, Tanja",
year = "2021",
abstract = "Microplastics are plastic fibers, particles or films with diameters smaller than 5 mm and they have shown different
effects on proteins [1]. Microplastics (MPs) are small in size, have low densities, can exist in the atmosphere for a
long time and can easily be spread by wind [2]. The objective of this study was to investigate adsorption affinity of
different types of MPs (polyethylene terephthalate (PET), polystyrene (PS) and polyvinyl chloride (PVC)) with
ovalbumin. In this study ovalbumin, isolated from chicken egg white, was used. Plastics were mixed with ovalbumin
for 1,2,4 and 19 h and then the absorbance of the remaining protein in the solution was measured at 280nm. In
addition, Langmuir isotherm mathematical model to calculate the adsorption affinity of ovalbumin for MPs was used.
We determined affinity constants by using Langmuir isotherm models for different particle size (PS 120 μm and PS
500 μm), different types of plastics (PET, PS and PVC) and pH values (3 and 7,2). Adsorption experiment results
showed that adsorption depends on type of plastics. Our results showed that PVC did not adsorb ovalbumin,
however, PET and PS have interacted with protein. Adsorption capacities of all analysed MPs increase with pH of
solution. Under different pH values, MPs and protein have different charges that may affect adsorption
characteristics. With increase of pH from 3 to 7, the affinity for protein adsorption increased 1.4 times for PS (smaller
in size) while for PS (bigger size) and PET protein affinity was two times higher at pH 3.",
publisher = "Sociedade Portuguesa de Química",
journal = "Book of Abstracts - XXI EuroFoodChem conference, 22nd-24th November, 2021, Virtual Congress",
title = "Binding affinity ovalbumin on different type of microplastics using Langmuir isotherm",
pages = "178-178",
url = "https://hdl.handle.net/21.15107/rcub_cer_7281"
}

Mutić, T., Gligorijević, N.,& Ćirković Veličković, T.. (2021). Binding affinity ovalbumin on different type of microplastics using Langmuir isotherm. in Book of Abstracts - XXI EuroFoodChem conference, 22nd-24th November, 2021, Virtual Congress
Sociedade Portuguesa de Química., 178-178.
https://hdl.handle.net/21.15107/rcub_cer_7281

Mutić T, Gligorijević N, Ćirković Veličković T. Binding affinity ovalbumin on different type of microplastics using Langmuir isotherm. in Book of Abstracts - XXI EuroFoodChem conference, 22nd-24th November, 2021, Virtual Congress. 2021;:178-178.
https://hdl.handle.net/21.15107/rcub_cer_7281 .

Mutić, Tamara, Gligorijević, Nikola, Ćirković Veličković, Tanja, "Binding affinity ovalbumin on different type of microplastics using Langmuir isotherm" in Book of Abstracts - XXI EuroFoodChem conference, 22nd-24th November, 2021, Virtual Congress (2021):178-178,
https://hdl.handle.net/21.15107/rcub_cer_7281 .