TY  - CONF
AU  - Radomirović, Mirjana
AU  - Gligorijević, Nikola
AU  - Stanić-Vučinić, Dragana
AU  - Rajković, Andreja
AU  - Ćirković Veličković, Tanja
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6668
AB  - Tropomyosin has been recognized as one of the most common allergens among shellfish
allergens. Sensitive and specific quantification of traces of allergens present in food
samples is of critical importance for people with food allergies. This study thus aimed to
develop a highly sensitive immuno-PCR method for detecting crustacean tropomyosin in
foods. Method couples conventional sandwich ELISA assay with real-time PCR
amplification of marker DNA. Monoclonal mouse anti-tropomyosin antibody was used as
a capture antibody, while polyclonal rabbit anti-tropomyosin antibody served as a
detection antibody in sandwich ELISA. A double-stranded amino-DNA molecule of 77
base pairs was covalently conjugated to a secondary goat anti-rabbit antibody and
subsequently amplified and quantified by real-time PCR. Tropomyosin was quantified
using highly purified natural shrimp tropomyosin as standard. The sensitivity of immuno-
PCR for quantification of tropomyosin was increased by up to 20-fold compared to
ELISA, demonstrating a ccuracy a s l ow a s 1 9.8 p g/mL. Recovery of tropomyosin in
vegetable soup as a food matrix was in the 87.7–115.6% range, with relative standard
deviations in the 5–24.5% range. Tropomyosin was also quantified in the commercially
available food products. Developed immuno-PCR technique thus shows the potential to be
a method of choice for specific and ultrasensitive detection of tropomyosin in food
samples, with the final aim of reducing risks of accidental food contamination.
PB  - Serbian Biochemical Society
C3  - Serbian Biochemical Society Twelfth Conference, International scientific meeting, “Biochemistry in Biotechnology,” September 21-23, 2023, Belgrade, Serbia
T1  - Immuno-PCR for crustacean tropomyosin quantification
SP  - 130
EP  - 130
UR  - https://hdl.handle.net/21.15107/rcub_cer_6668
ER  - 

@conference{
author = "Radomirović, Mirjana and Gligorijević, Nikola and Stanić-Vučinić, Dragana and Rajković, Andreja and Ćirković Veličković, Tanja",
year = "2023",
abstract = "Tropomyosin has been recognized as one of the most common allergens among shellfish
allergens. Sensitive and specific quantification of traces of allergens present in food
samples is of critical importance for people with food allergies. This study thus aimed to
develop a highly sensitive immuno-PCR method for detecting crustacean tropomyosin in
foods. Method couples conventional sandwich ELISA assay with real-time PCR
amplification of marker DNA. Monoclonal mouse anti-tropomyosin antibody was used as
a capture antibody, while polyclonal rabbit anti-tropomyosin antibody served as a
detection antibody in sandwich ELISA. A double-stranded amino-DNA molecule of 77
base pairs was covalently conjugated to a secondary goat anti-rabbit antibody and
subsequently amplified and quantified by real-time PCR. Tropomyosin was quantified
using highly purified natural shrimp tropomyosin as standard. The sensitivity of immuno-
PCR for quantification of tropomyosin was increased by up to 20-fold compared to
ELISA, demonstrating a ccuracy a s l ow a s 1 9.8 p g/mL. Recovery of tropomyosin in
vegetable soup as a food matrix was in the 87.7–115.6% range, with relative standard
deviations in the 5–24.5% range. Tropomyosin was also quantified in the commercially
available food products. Developed immuno-PCR technique thus shows the potential to be
a method of choice for specific and ultrasensitive detection of tropomyosin in food
samples, with the final aim of reducing risks of accidental food contamination.",
publisher = "Serbian Biochemical Society",
journal = "Serbian Biochemical Society Twelfth Conference, International scientific meeting, “Biochemistry in Biotechnology,” September 21-23, 2023, Belgrade, Serbia",
title = "Immuno-PCR for crustacean tropomyosin quantification",
pages = "130-130",
url = "https://hdl.handle.net/21.15107/rcub_cer_6668"
}

Radomirović, M., Gligorijević, N., Stanić-Vučinić, D., Rajković, A.,& Ćirković Veličković, T.. (2023). Immuno-PCR for crustacean tropomyosin quantification. in Serbian Biochemical Society Twelfth Conference, International scientific meeting, “Biochemistry in Biotechnology,” September 21-23, 2023, Belgrade, Serbia
Serbian Biochemical Society., 130-130.
https://hdl.handle.net/21.15107/rcub_cer_6668

Radomirović M, Gligorijević N, Stanić-Vučinić D, Rajković A, Ćirković Veličković T. Immuno-PCR for crustacean tropomyosin quantification. in Serbian Biochemical Society Twelfth Conference, International scientific meeting, “Biochemistry in Biotechnology,” September 21-23, 2023, Belgrade, Serbia. 2023;:130-130.
https://hdl.handle.net/21.15107/rcub_cer_6668 .

Radomirović, Mirjana, Gligorijević, Nikola, Stanić-Vučinić, Dragana, Rajković, Andreja, Ćirković Veličković, Tanja, "Immuno-PCR for crustacean tropomyosin quantification" in Serbian Biochemical Society Twelfth Conference, International scientific meeting, “Biochemistry in Biotechnology,” September 21-23, 2023, Belgrade, Serbia (2023):130-130,
https://hdl.handle.net/21.15107/rcub_cer_6668 .