TY  - JOUR
AU  - Elferjane, Muna
AU  - Batinić, Petar
AU  - Čutović, Natalija
AU  - Milošević, Milena
AU  - Ćirić, Vojislav
AU  - Marinković, Aleksandar
AU  - Jovanović, Aleksandra
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7411
AB  - Vaccinium myrtillus L. (bilberry, Ericaceae), a perennial, wild, and small deciduous shrub that grows in the mountains and forests of Europe. The leaf extracts are widely used in traditional medicine due to their astringent, antiseptic, antioxidant, anti-inflammatory, hypolipidemic, and hypoglycemic activities. Microwave-assisted extraction provides various benefits, including reducing solvent consumption and extraction time and increasing extraction yield. In the present study, bilberry extracts were prepared using dried leaves, pure ethanol, or an ethanol-water mixture, and different temperatures in a microwave reactor (60, 100, and 160 ◦C). The extracts were examined in terms of total polyphenol content (TPC) and antioxidant activity. The TPC of the 96% ethanol extracts rose with the increase of the extraction temperature; the extract obtained at 60 ◦C (37.2 ± 0.5 mg gallic acid equivalents (GAE)/g of plant material)<the extract at 100 ◦C (46.6 ± 0.3 GAE/g)<the extract at 160 ◦C (55.1 ± 0.5 mg/g). The TPC of the 50% ethanol extract prepared at 60 ◦C was significantly lower (54.9 ± 1.0 mg GAE/g) in comparison to the extracts obtained at 100 and 160 ◦C (58.3 ± 1.0 and 58.0 ± 1.5 mg GAE/g, respectively). ABTS antioxidant capacity was higher in the 50% ethanol extracts compared to 96% ethanol parallels, while in the DPPH assay, there was no statistically significant difference between the 50 and 96% ethanol extracts. Due to higher TPC and anti-ABTS activity and reduced consumption of organic solvent, a 50% ethanol extract of V. myrtillus was favored, while the optimal temperature was 60 ◦C for the extract with the highest antioxidant capacity and 100 ◦C for the extract with the highest amount of polyphenols.
PB  - Institute for Medicinal Plant Research "Dr. Josif Pančić"
T2  - Lekovite sirovine
T1  - Microwawe-assisted extraction of antioxidant compounds from Vaccinium Myrtillus leaves
VL  - 43
IS  - 1
SP  - 1
EP  - 4
DO  - 10.61652/leksir2343e135E
ER  - 

@article{
author = "Elferjane, Muna and Batinić, Petar and Čutović, Natalija and Milošević, Milena and Ćirić, Vojislav and Marinković, Aleksandar and Jovanović, Aleksandra",
year = "2023",
abstract = "Vaccinium myrtillus L. (bilberry, Ericaceae), a perennial, wild, and small deciduous shrub that grows in the mountains and forests of Europe. The leaf extracts are widely used in traditional medicine due to their astringent, antiseptic, antioxidant, anti-inflammatory, hypolipidemic, and hypoglycemic activities. Microwave-assisted extraction provides various benefits, including reducing solvent consumption and extraction time and increasing extraction yield. In the present study, bilberry extracts were prepared using dried leaves, pure ethanol, or an ethanol-water mixture, and different temperatures in a microwave reactor (60, 100, and 160 ◦C). The extracts were examined in terms of total polyphenol content (TPC) and antioxidant activity. The TPC of the 96% ethanol extracts rose with the increase of the extraction temperature; the extract obtained at 60 ◦C (37.2 ± 0.5 mg gallic acid equivalents (GAE)/g of plant material)<the extract at 100 ◦C (46.6 ± 0.3 GAE/g)<the extract at 160 ◦C (55.1 ± 0.5 mg/g). The TPC of the 50% ethanol extract prepared at 60 ◦C was significantly lower (54.9 ± 1.0 mg GAE/g) in comparison to the extracts obtained at 100 and 160 ◦C (58.3 ± 1.0 and 58.0 ± 1.5 mg GAE/g, respectively). ABTS antioxidant capacity was higher in the 50% ethanol extracts compared to 96% ethanol parallels, while in the DPPH assay, there was no statistically significant difference between the 50 and 96% ethanol extracts. Due to higher TPC and anti-ABTS activity and reduced consumption of organic solvent, a 50% ethanol extract of V. myrtillus was favored, while the optimal temperature was 60 ◦C for the extract with the highest antioxidant capacity and 100 ◦C for the extract with the highest amount of polyphenols.",
publisher = "Institute for Medicinal Plant Research "Dr. Josif Pančić"",
journal = "Lekovite sirovine",
title = "Microwawe-assisted extraction of antioxidant compounds from Vaccinium Myrtillus leaves",
volume = "43",
number = "1",
pages = "1-4",
doi = "10.61652/leksir2343e135E"
}

Elferjane, M., Batinić, P., Čutović, N., Milošević, M., Ćirić, V., Marinković, A.,& Jovanović, A.. (2023). Microwawe-assisted extraction of antioxidant compounds from Vaccinium Myrtillus leaves. in Lekovite sirovine
Institute for Medicinal Plant Research "Dr. Josif Pančić"., 43(1), 1-4.
https://doi.org/10.61652/leksir2343e135E

Elferjane M, Batinić P, Čutović N, Milošević M, Ćirić V, Marinković A, Jovanović A. Microwawe-assisted extraction of antioxidant compounds from Vaccinium Myrtillus leaves. in Lekovite sirovine. 2023;43(1):1-4.
doi:10.61652/leksir2343e135E .

Elferjane, Muna, Batinić, Petar, Čutović, Natalija, Milošević, Milena, Ćirić, Vojislav, Marinković, Aleksandar, Jovanović, Aleksandra, "Microwawe-assisted extraction of antioxidant compounds from Vaccinium Myrtillus leaves" in Lekovite sirovine, 43, no. 1 (2023):1-4,
https://doi.org/10.61652/leksir2343e135E . .

TY  - CONF
AU  - Batinić, Petar
AU  - Jovanović, Aleksandra A.
AU  - Rajab Elferjane, Muna
AU  - Čutović, Natalija
AU  - Milošević, Milena
AU  - Marinković, Aleksandar
AU  - Bugarski, Branko
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7332
AB  - Vaccinium myrtillus L.(Ericaceae), a perennial, wild, and small deciduous shrub that grows in the mountains and forests of Europe, contains anthocyanins, phenolic acids, flavonoids, fatty acids, stilbenes, iridoid glycosides, dietary fibers, vitamins, and minerals. The leaves' extracts are widely used in traditional medicine due to their astringent, antiseptic, antioxidant, anti-inflammatory, skin-rejuvenating, lipid-lowering, hypolipidemic, and hypoglycemic activities. The novel extraction techniques, including ultrasound-assisted extraction, provide various benefits, such as reducing solvent consumption and extraction time and increasing extraction yield, and quality. Hence, in the present study, V. myrtillus extracts were prepared using dried leaves (0.66 g), 50% ethanol as the extraction solvent (20 mL), and maceration (60 min) or ultrasound-assisted extraction (ultrasound probe, amplitude of 60% for 5 min). The obtained extracts were examined in terms of total polyphenol content (TPC) and antioxidant activity (ABTS, DPPH, FRAP, and CUPRAC assays). The TPC of the extract prepared using maceration was 55.2±0.7 mg gallic acid equivalents (GAE)/g of plant material, while the TPC of the extract prepared using an ultrasound probe was 55.6±1.0 mg GAE/g. The DPPH radical scavenging activities of the extracts correlated with the TPC and amounted to 1.81±0.05 mg/mL for macerate and 1.79±0.02 mg/mL for the extract obtained using an ultrasound probe, whereas ABTS antioxidant capacity did not correlate with the polyphenol concentration, 31.4±0.9 µmol Trolox equivalents (TE)/g for the macerate and 43.4±1.1 µmol TE/g for the extract from the ultrasound-assisted extraction. According to the results of FRAP and CUPRAC assays, the antioxidant potential was similar for both extracts (15.3±0.2 and 15.5±0.2 µmol Fe2+/g and 45.7±0.5 and 46.0±0.7 µmol TE/g, respectively). Due to higher ABTS radical scavenging potential and significantly shorter extraction time, V. myrtillus extract prepared using an ultrasound probe was favored. The present research was an initial step in the preparation of V. myrtillus extracts which can be potentially implemented in food, pharmaceutical, and cosmetic formulations.
PB  - Zvornik : University of East Sarajevo, Faculty of Technology
C3  - Proceedings - VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM 2023, March 20-23 2023, Jahorina
T1  - Comparison of maceration and ultrasound-assisted extraction of antioxidant compounds from Vaccinium myrtillus L.
SP  - 172
EP  - 178
UR  - https://hdl.handle.net/21.15107/rcub_cer_7332
ER  - 

@conference{
author = "Batinić, Petar and Jovanović, Aleksandra A. and Rajab Elferjane, Muna and Čutović, Natalija and Milošević, Milena and Marinković, Aleksandar and Bugarski, Branko",
year = "2023",
abstract = "Vaccinium myrtillus L.(Ericaceae), a perennial, wild, and small deciduous shrub that grows in the mountains and forests of Europe, contains anthocyanins, phenolic acids, flavonoids, fatty acids, stilbenes, iridoid glycosides, dietary fibers, vitamins, and minerals. The leaves' extracts are widely used in traditional medicine due to their astringent, antiseptic, antioxidant, anti-inflammatory, skin-rejuvenating, lipid-lowering, hypolipidemic, and hypoglycemic activities. The novel extraction techniques, including ultrasound-assisted extraction, provide various benefits, such as reducing solvent consumption and extraction time and increasing extraction yield, and quality. Hence, in the present study, V. myrtillus extracts were prepared using dried leaves (0.66 g), 50% ethanol as the extraction solvent (20 mL), and maceration (60 min) or ultrasound-assisted extraction (ultrasound probe, amplitude of 60% for 5 min). The obtained extracts were examined in terms of total polyphenol content (TPC) and antioxidant activity (ABTS, DPPH, FRAP, and CUPRAC assays). The TPC of the extract prepared using maceration was 55.2±0.7 mg gallic acid equivalents (GAE)/g of plant material, while the TPC of the extract prepared using an ultrasound probe was 55.6±1.0 mg GAE/g. The DPPH radical scavenging activities of the extracts correlated with the TPC and amounted to 1.81±0.05 mg/mL for macerate and 1.79±0.02 mg/mL for the extract obtained using an ultrasound probe, whereas ABTS antioxidant capacity did not correlate with the polyphenol concentration, 31.4±0.9 µmol Trolox equivalents (TE)/g for the macerate and 43.4±1.1 µmol TE/g for the extract from the ultrasound-assisted extraction. According to the results of FRAP and CUPRAC assays, the antioxidant potential was similar for both extracts (15.3±0.2 and 15.5±0.2 µmol Fe2+/g and 45.7±0.5 and 46.0±0.7 µmol TE/g, respectively). Due to higher ABTS radical scavenging potential and significantly shorter extraction time, V. myrtillus extract prepared using an ultrasound probe was favored. The present research was an initial step in the preparation of V. myrtillus extracts which can be potentially implemented in food, pharmaceutical, and cosmetic formulations.",
publisher = "Zvornik : University of East Sarajevo, Faculty of Technology",
journal = "Proceedings - VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM 2023, March 20-23 2023, Jahorina",
title = "Comparison of maceration and ultrasound-assisted extraction of antioxidant compounds from Vaccinium myrtillus L.",
pages = "172-178",
url = "https://hdl.handle.net/21.15107/rcub_cer_7332"
}

Batinić, P., Jovanović, A. A., Rajab Elferjane, M., Čutović, N., Milošević, M., Marinković, A.,& Bugarski, B.. (2023). Comparison of maceration and ultrasound-assisted extraction of antioxidant compounds from Vaccinium myrtillus L.. in Proceedings - VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM 2023, March 20-23 2023, Jahorina
Zvornik : University of East Sarajevo, Faculty of Technology., 172-178.
https://hdl.handle.net/21.15107/rcub_cer_7332

Batinić P, Jovanović AA, Rajab Elferjane M, Čutović N, Milošević M, Marinković A, Bugarski B. Comparison of maceration and ultrasound-assisted extraction of antioxidant compounds from Vaccinium myrtillus L.. in Proceedings - VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM 2023, March 20-23 2023, Jahorina. 2023;:172-178.
https://hdl.handle.net/21.15107/rcub_cer_7332 .

Batinić, Petar, Jovanović, Aleksandra A., Rajab Elferjane, Muna, Čutović, Natalija, Milošević, Milena, Marinković, Aleksandar, Bugarski, Branko, "Comparison of maceration and ultrasound-assisted extraction of antioxidant compounds from Vaccinium myrtillus L." in Proceedings - VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM 2023, March 20-23 2023, Jahorina (2023):172-178,
https://hdl.handle.net/21.15107/rcub_cer_7332 .

TY  - CONF
AU  - Milošević, Milena
AU  - Marinković, Aleksandar
AU  - Batinić, Petar M.
AU  - Đuričković, Ivan
AU  - Jovanović, Aleksandra A.
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7336
AB  - Thymus serpyllum L. (wild thyme) extracts were prepared using 1 g of plant material and three natural deep eutectic solvents (30 mL, malic acid+maltose, lactic acid+menthol, or citric acid+glycerol with 50% of water) in maceration (90 min). The extracts were characterized in terms of total polyphenol content (TPC), total protein content, extraction yield, zeta potential, conductivity, pH, density, surface tension, and viscosity. TPC was the highest in citric acid+glycerol extract (35.06±1.13 mg gallic acid equivalents (GAE)/g of plant material), whereas malic acid+maltose and lactic acid+menthol extracts possessed similar TPC (29.62±1.11 and 28.94±1.15 mg GAE/g, respectively). Total protein content amounted to 5.55±0.20 mg/g in citric acid+glycerol extract, while significantly lower values were determined in malic acid+maltose and lactic acid+menthol extracts (1.22±0.30 and 1.73±0.18 mg/g, respectively). The extraction yield was the highest for citric acid+glycerol extract, 1.57±0.11%, followed by malic acid+maltose and lactic acid+menthol extracts, 1.28±0.10 and 1.27±0.13%, respectively. Zeta potential (absolute value, as a predictor of potential application in water treatment) was low in all extracts (0.86±0.06 mV for citric acid+glycerol, -0.35±0.09 mV for lactic acid+menthol, and -0.17±0.05 mV for malic acid+maltose extract). The conductivity (as a predictor of antioxidant capacity) was in the range of 2.35±0.21 and 2.71±0.29 mS/cm (citric acid+glycerol and malic acid+maltose extracts) to 5.70±0.23 mS/cm (lactic acid+menthol extract). pH ranged from 1.44 in citric acid+glycerol extract to 1.78 and 1.85 in malic acid+maltose and lactic acid+menthol extracts. Density varied from 1.067±0.003 g/mL for lactic acid+menthol extract to 1.169±0.002 and 1.195±0.005 g/mL for citric acid+glycerol and malic acid+maltose extracts. Surface tension was the highest in citric acid+glycerol extract (38.0±0.4 mN/m), followed by malic acid+maltose and lactic acid+menthol extracts (31.5±0.4 and 25.9±0.1 mN/m, respectively). The viscosity of lactic acid+menthol extract was significantly lower (1.83±0.30 mPa•s) in comparison to malic acid+maltose and citric acid+glycerol extracts (6.64±0.15 and 7.84±0.10 mPa•s, respectively). The highest TPC, total proteins, and extraction yield were measured in citric acid+glycerol extract, while lactic acid+menthol extract possessed the highest conductivity and the lowest density, surface tension, and viscosity. Therefore, the constitution of natural deep eutectic solvent should be optimized depending on the future application of T. serpyllum extract.
PB  - Zvornik : University of East Sarajevo, Faculty of Technology
C3  - Proceedings - VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM 2023, March 20-23 2023, Jahorina
T1  - Physicochemical characterisation of Thymus serpyllum extracts prepared using natural deep eutectic solvents
SP  - 258
EP  - 266
UR  - https://hdl.handle.net/21.15107/rcub_cer_7336
ER  - 

@conference{
author = "Milošević, Milena and Marinković, Aleksandar and Batinić, Petar M. and Đuričković, Ivan and Jovanović, Aleksandra A.",
year = "2023",
abstract = "Thymus serpyllum L. (wild thyme) extracts were prepared using 1 g of plant material and three natural deep eutectic solvents (30 mL, malic acid+maltose, lactic acid+menthol, or citric acid+glycerol with 50% of water) in maceration (90 min). The extracts were characterized in terms of total polyphenol content (TPC), total protein content, extraction yield, zeta potential, conductivity, pH, density, surface tension, and viscosity. TPC was the highest in citric acid+glycerol extract (35.06±1.13 mg gallic acid equivalents (GAE)/g of plant material), whereas malic acid+maltose and lactic acid+menthol extracts possessed similar TPC (29.62±1.11 and 28.94±1.15 mg GAE/g, respectively). Total protein content amounted to 5.55±0.20 mg/g in citric acid+glycerol extract, while significantly lower values were determined in malic acid+maltose and lactic acid+menthol extracts (1.22±0.30 and 1.73±0.18 mg/g, respectively). The extraction yield was the highest for citric acid+glycerol extract, 1.57±0.11%, followed by malic acid+maltose and lactic acid+menthol extracts, 1.28±0.10 and 1.27±0.13%, respectively. Zeta potential (absolute value, as a predictor of potential application in water treatment) was low in all extracts (0.86±0.06 mV for citric acid+glycerol, -0.35±0.09 mV for lactic acid+menthol, and -0.17±0.05 mV for malic acid+maltose extract). The conductivity (as a predictor of antioxidant capacity) was in the range of 2.35±0.21 and 2.71±0.29 mS/cm (citric acid+glycerol and malic acid+maltose extracts) to 5.70±0.23 mS/cm (lactic acid+menthol extract). pH ranged from 1.44 in citric acid+glycerol extract to 1.78 and 1.85 in malic acid+maltose and lactic acid+menthol extracts. Density varied from 1.067±0.003 g/mL for lactic acid+menthol extract to 1.169±0.002 and 1.195±0.005 g/mL for citric acid+glycerol and malic acid+maltose extracts. Surface tension was the highest in citric acid+glycerol extract (38.0±0.4 mN/m), followed by malic acid+maltose and lactic acid+menthol extracts (31.5±0.4 and 25.9±0.1 mN/m, respectively). The viscosity of lactic acid+menthol extract was significantly lower (1.83±0.30 mPa•s) in comparison to malic acid+maltose and citric acid+glycerol extracts (6.64±0.15 and 7.84±0.10 mPa•s, respectively). The highest TPC, total proteins, and extraction yield were measured in citric acid+glycerol extract, while lactic acid+menthol extract possessed the highest conductivity and the lowest density, surface tension, and viscosity. Therefore, the constitution of natural deep eutectic solvent should be optimized depending on the future application of T. serpyllum extract.",
publisher = "Zvornik : University of East Sarajevo, Faculty of Technology",
journal = "Proceedings - VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM 2023, March 20-23 2023, Jahorina",
title = "Physicochemical characterisation of Thymus serpyllum extracts prepared using natural deep eutectic solvents",
pages = "258-266",
url = "https://hdl.handle.net/21.15107/rcub_cer_7336"
}

Milošević, M., Marinković, A., Batinić, P. M., Đuričković, I.,& Jovanović, A. A.. (2023). Physicochemical characterisation of Thymus serpyllum extracts prepared using natural deep eutectic solvents. in Proceedings - VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM 2023, March 20-23 2023, Jahorina
Zvornik : University of East Sarajevo, Faculty of Technology., 258-266.
https://hdl.handle.net/21.15107/rcub_cer_7336

Milošević M, Marinković A, Batinić PM, Đuričković I, Jovanović AA. Physicochemical characterisation of Thymus serpyllum extracts prepared using natural deep eutectic solvents. in Proceedings - VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM 2023, March 20-23 2023, Jahorina. 2023;:258-266.
https://hdl.handle.net/21.15107/rcub_cer_7336 .

Milošević, Milena, Marinković, Aleksandar, Batinić, Petar M., Đuričković, Ivan, Jovanović, Aleksandra A., "Physicochemical characterisation of Thymus serpyllum extracts prepared using natural deep eutectic solvents" in Proceedings - VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM 2023, March 20-23 2023, Jahorina (2023):258-266,
https://hdl.handle.net/21.15107/rcub_cer_7336 .

TY  - CONF
AU  - Milošević, Milena
AU  - Jovanović, Aleksandra A.
AU  - Batinić, Petar M.
AU  - Grujić, Dragana
AU  - Knežević, Nataša
AU  - Marinković, Aleksandar
AU  - Milanović, Jovana
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7338
AB  - The plants contain a large number of various compounds that exhibit significant antioxidant 
activity. Among them, polyphenols and flavonoids are the most studied compounds with different 
biological properties, including antioxidant activity. The functionalization of cotton fabric with natural compounds from plants results in the material with improved antioxidative properties. In this study, cotton fabrics treated with ethanol and water Thymus serpyllum L. (wild thyme) extracts were investigated in terms of antioxidant properties and chemical composition. In vitro antioxidant capacity of ethanol and water extracts, and the cotton fabric before and after functionalization was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH•), and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS•+) radicals scavenging capacity assays. FTIR and UV–Vis spectroscopy elucidated the structure of the cotton fabric, extracts, and functionalized cotton fabric. FTIR spectroscopy demonstrated that the structure of cellulose was dominated in cotton, and also proved the presence of a low percentage of protein, pectin, oil, fat, and wax, as well as, binding of compounds from the extract by cross-linking with long chains of structure cotton. In dry extracts, the presence of various chemical functional groups was confirmed. UV-Vis analysis identified the active components in T. serpyllum extracts. Namely, absorption values for both spectra (water and ethanol samples) at 215-253 nm, 263-384 nm, are specific for phenolic acids, and flavonoids. Also, one of the aims of the study was to investigate the influence of various extracts (water and ethanol samples) on the antioxidant activity of cotton fabric. All extracts demonstrated excellent antioxidant properties, more potent than the reference antioxidant (ascorbic acid) in both antioxidant assays (between 85-86% in DPPH and 94-96% in ABTS assays). Pure T. serpyllum water extract was the most potent in the DPPH assay (86%), while pure ethanolic extract was the most potent antioxidant in the ABTS assay (96%). The antioxidant capacity of raw cotton fabric was insufficient (4%). On the other hand, in the DPPH assay, samples of cotton fabric with extract showed moderate activity with scavenging activity of 48% and 45% for ethanol and water extracts, respectively. Additionally,
in the ABTS assay, the antioxidant activity of treated cotton fabric was 51% and 46% for ethanol 
and water extracts, respectively.
PB  - Zvornik : University of East Sarajevo, Faculty of Technology
C3  - Proceedings - VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM 2023, March 20-23 2023, Jahorina
T1  - In vitro antioxidant activity of cotton fabric treated with ethanol and water Thymus serpyllum L. (wild thyme) extracts
SP  - 128
EP  - 135
UR  - https://hdl.handle.net/21.15107/rcub_cer_7338
ER  - 

@conference{
author = "Milošević, Milena and Jovanović, Aleksandra A. and Batinić, Petar M. and Grujić, Dragana and Knežević, Nataša and Marinković, Aleksandar and Milanović, Jovana",
year = "2023",
abstract = "The plants contain a large number of various compounds that exhibit significant antioxidant 
activity. Among them, polyphenols and flavonoids are the most studied compounds with different 
biological properties, including antioxidant activity. The functionalization of cotton fabric with natural compounds from plants results in the material with improved antioxidative properties. In this study, cotton fabrics treated with ethanol and water Thymus serpyllum L. (wild thyme) extracts were investigated in terms of antioxidant properties and chemical composition. In vitro antioxidant capacity of ethanol and water extracts, and the cotton fabric before and after functionalization was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH•), and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS•+) radicals scavenging capacity assays. FTIR and UV–Vis spectroscopy elucidated the structure of the cotton fabric, extracts, and functionalized cotton fabric. FTIR spectroscopy demonstrated that the structure of cellulose was dominated in cotton, and also proved the presence of a low percentage of protein, pectin, oil, fat, and wax, as well as, binding of compounds from the extract by cross-linking with long chains of structure cotton. In dry extracts, the presence of various chemical functional groups was confirmed. UV-Vis analysis identified the active components in T. serpyllum extracts. Namely, absorption values for both spectra (water and ethanol samples) at 215-253 nm, 263-384 nm, are specific for phenolic acids, and flavonoids. Also, one of the aims of the study was to investigate the influence of various extracts (water and ethanol samples) on the antioxidant activity of cotton fabric. All extracts demonstrated excellent antioxidant properties, more potent than the reference antioxidant (ascorbic acid) in both antioxidant assays (between 85-86% in DPPH and 94-96% in ABTS assays). Pure T. serpyllum water extract was the most potent in the DPPH assay (86%), while pure ethanolic extract was the most potent antioxidant in the ABTS assay (96%). The antioxidant capacity of raw cotton fabric was insufficient (4%). On the other hand, in the DPPH assay, samples of cotton fabric with extract showed moderate activity with scavenging activity of 48% and 45% for ethanol and water extracts, respectively. Additionally,
in the ABTS assay, the antioxidant activity of treated cotton fabric was 51% and 46% for ethanol 
and water extracts, respectively.",
publisher = "Zvornik : University of East Sarajevo, Faculty of Technology",
journal = "Proceedings - VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM 2023, March 20-23 2023, Jahorina",
title = "In vitro antioxidant activity of cotton fabric treated with ethanol and water Thymus serpyllum L. (wild thyme) extracts",
pages = "128-135",
url = "https://hdl.handle.net/21.15107/rcub_cer_7338"
}

Milošević, M., Jovanović, A. A., Batinić, P. M., Grujić, D., Knežević, N., Marinković, A.,& Milanović, J.. (2023). In vitro antioxidant activity of cotton fabric treated with ethanol and water Thymus serpyllum L. (wild thyme) extracts. in Proceedings - VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM 2023, March 20-23 2023, Jahorina
Zvornik : University of East Sarajevo, Faculty of Technology., 128-135.
https://hdl.handle.net/21.15107/rcub_cer_7338

Milošević M, Jovanović AA, Batinić PM, Grujić D, Knežević N, Marinković A, Milanović J. In vitro antioxidant activity of cotton fabric treated with ethanol and water Thymus serpyllum L. (wild thyme) extracts. in Proceedings - VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM 2023, March 20-23 2023, Jahorina. 2023;:128-135.
https://hdl.handle.net/21.15107/rcub_cer_7338 .

Milošević, Milena, Jovanović, Aleksandra A., Batinić, Petar M., Grujić, Dragana, Knežević, Nataša, Marinković, Aleksandar, Milanović, Jovana, "In vitro antioxidant activity of cotton fabric treated with ethanol and water Thymus serpyllum L. (wild thyme) extracts" in Proceedings - VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM 2023, March 20-23 2023, Jahorina (2023):128-135,
https://hdl.handle.net/21.15107/rcub_cer_7338 .

TY  - CONF
AU  - Jovanović, Aleksandra A.
AU  - Rajab Elferjane, Muna
AU  - Milošević, Milena
AU  - Batinić, Petar
AU  - Bošnjaković, Jovana
AU  - Rančić, Milica
AU  - Marinković, Aleksandar
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7333
AB  - Wild thyme contains biologically active compounds, particularly polyphenols (flavonoids and phenolic acids) that exert antitumor, antimicrobial, anti-inflammatory, antioxidant, immunomodulatory, analgesic, and spasmolytic activities. Nevertheless, the mentioned bioactive compounds have low stability, solubility, and bioavailability, thus their application is limited. Liposomal particles have been widely used for the encapsulation of bioactive components, due to their high structural integrity, stability during storage, and controlled release capability. In the present study, phospholipid-cholesterol liposomal particles, as the carrier for wild thyme extract, were developed. The stability of extract-loaded liposomes was monitored for 21 days by measuring vesicle size, polydispersity index (PDI), and zeta potential. The particle size and PDI of extract-loaded liposomes did not change drastically during 21 days of storage and amounted to ~450 nm and ~0.155, respectively. The zeta potential varied in the liposomes and started to decrease after 7 days of storage (from -21.0 mV to 20.3 mV, without a statistically significant difference), while the zeta potential value after 21 days was statistically significantly lower in comparison to the 1st day (19.3 mV). The beneficial effects of polyphenols on human health, as well as showed storage stability of the prepared liposomes highlight the use of the wild thyme extract-loaded phospholipid-cholesterol liposomal particles for potential application in food, pharmaceutical, and cosmetic industries.
PB  - Serbian society of corrosion and materials protection (UISKoZaM) / Udruženje inženjera Srbije za koroziju i zaštitu materijala
C3  - Proceedings - XXIV YuCorr International Conference, Meeting Point of the Science and Practice in the Fields of Corrosion, Materials and Environmental Protection, May 28-31, 2023, Divčibare, Serbia
T1  - The Stability of Wild Thyme Extract-Loaded Phospholipid-Cholesterol Liposomal Particles
T1  - Stabilnost lipozomalnih čestica sa inkapsuliranim ekstraktom majčine dušice
SP  - 96
EP  - 101
UR  - https://hdl.handle.net/21.15107/rcub_cer_7333
ER  - 

@conference{
author = "Jovanović, Aleksandra A. and Rajab Elferjane, Muna and Milošević, Milena and Batinić, Petar and Bošnjaković, Jovana and Rančić, Milica and Marinković, Aleksandar",
year = "2023",
abstract = "Wild thyme contains biologically active compounds, particularly polyphenols (flavonoids and phenolic acids) that exert antitumor, antimicrobial, anti-inflammatory, antioxidant, immunomodulatory, analgesic, and spasmolytic activities. Nevertheless, the mentioned bioactive compounds have low stability, solubility, and bioavailability, thus their application is limited. Liposomal particles have been widely used for the encapsulation of bioactive components, due to their high structural integrity, stability during storage, and controlled release capability. In the present study, phospholipid-cholesterol liposomal particles, as the carrier for wild thyme extract, were developed. The stability of extract-loaded liposomes was monitored for 21 days by measuring vesicle size, polydispersity index (PDI), and zeta potential. The particle size and PDI of extract-loaded liposomes did not change drastically during 21 days of storage and amounted to ~450 nm and ~0.155, respectively. The zeta potential varied in the liposomes and started to decrease after 7 days of storage (from -21.0 mV to 20.3 mV, without a statistically significant difference), while the zeta potential value after 21 days was statistically significantly lower in comparison to the 1st day (19.3 mV). The beneficial effects of polyphenols on human health, as well as showed storage stability of the prepared liposomes highlight the use of the wild thyme extract-loaded phospholipid-cholesterol liposomal particles for potential application in food, pharmaceutical, and cosmetic industries.",
publisher = "Serbian society of corrosion and materials protection (UISKoZaM) / Udruženje inženjera Srbije za koroziju i zaštitu materijala",
journal = "Proceedings - XXIV YuCorr International Conference, Meeting Point of the Science and Practice in the Fields of Corrosion, Materials and Environmental Protection, May 28-31, 2023, Divčibare, Serbia",
title = "The Stability of Wild Thyme Extract-Loaded Phospholipid-Cholesterol Liposomal Particles, Stabilnost lipozomalnih čestica sa inkapsuliranim ekstraktom majčine dušice",
pages = "96-101",
url = "https://hdl.handle.net/21.15107/rcub_cer_7333"
}

Jovanović, A. A., Rajab Elferjane, M., Milošević, M., Batinić, P., Bošnjaković, J., Rančić, M.,& Marinković, A.. (2023). The Stability of Wild Thyme Extract-Loaded Phospholipid-Cholesterol Liposomal Particles. in Proceedings - XXIV YuCorr International Conference, Meeting Point of the Science and Practice in the Fields of Corrosion, Materials and Environmental Protection, May 28-31, 2023, Divčibare, Serbia
Serbian society of corrosion and materials protection (UISKoZaM) / Udruženje inženjera Srbije za koroziju i zaštitu materijala., 96-101.
https://hdl.handle.net/21.15107/rcub_cer_7333

Jovanović AA, Rajab Elferjane M, Milošević M, Batinić P, Bošnjaković J, Rančić M, Marinković A. The Stability of Wild Thyme Extract-Loaded Phospholipid-Cholesterol Liposomal Particles. in Proceedings - XXIV YuCorr International Conference, Meeting Point of the Science and Practice in the Fields of Corrosion, Materials and Environmental Protection, May 28-31, 2023, Divčibare, Serbia. 2023;:96-101.
https://hdl.handle.net/21.15107/rcub_cer_7333 .

Jovanović, Aleksandra A., Rajab Elferjane, Muna, Milošević, Milena, Batinić, Petar, Bošnjaković, Jovana, Rančić, Milica, Marinković, Aleksandar, "The Stability of Wild Thyme Extract-Loaded Phospholipid-Cholesterol Liposomal Particles" in Proceedings - XXIV YuCorr International Conference, Meeting Point of the Science and Practice in the Fields of Corrosion, Materials and Environmental Protection, May 28-31, 2023, Divčibare, Serbia (2023):96-101,
https://hdl.handle.net/21.15107/rcub_cer_7333 .

TY  - JOUR
AU  - Živković, Jelena
AU  - Jadranin, Milka
AU  - Przić, Zoran
AU  - Marković, Nebojša
AU  - Sokolović, Danijel
AU  - Savikin, Katarina
AU  - Menković, Nebojša
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4249
AB  - In this study we have analyzed the anthocyanin composition of skin extracts of three red grape varieties Prokupac, Evita and Čokot Zemun in order to distinguish these cultivars based on their anthocyanin profile. Also, mechanical analysis of grape bunches and berries was performed. According to our results, seventeen anthocyanins were identified using LC‐MS technique and quantitative differences were recorded using HPLC‐DAD method. The highest content of total anthocyanins was obtained for Evita variety and the lowest one was recorded in Prokupac. Also, clear differences were observed in anthocyanins ratios. In comparison to Prokupac and Evita varieties, Čokot Zemun was characterized with a high content of cumaroyl derivatives of anthocyanin compounds, while high levels of acetylated derivatives were recorded in Prokupac. Data reported in this study represent a certain contribution to a database of mechanical properties and chemical composition of grape varieties originating from Balkan.
T2  - Chemistry & Biodiversity
T1  - Varietal differences of Prokupac, Evita and Čokot Zemun based on their anthocyanins content in grape skin extract
DO  - 10.1002/cbdv.202000858
ER  - 

@article{
author = "Živković, Jelena and Jadranin, Milka and Przić, Zoran and Marković, Nebojša and Sokolović, Danijel and Savikin, Katarina and Menković, Nebojša",
year = "2021",
abstract = "In this study we have analyzed the anthocyanin composition of skin extracts of three red grape varieties Prokupac, Evita and Čokot Zemun in order to distinguish these cultivars based on their anthocyanin profile. Also, mechanical analysis of grape bunches and berries was performed. According to our results, seventeen anthocyanins were identified using LC‐MS technique and quantitative differences were recorded using HPLC‐DAD method. The highest content of total anthocyanins was obtained for Evita variety and the lowest one was recorded in Prokupac. Also, clear differences were observed in anthocyanins ratios. In comparison to Prokupac and Evita varieties, Čokot Zemun was characterized with a high content of cumaroyl derivatives of anthocyanin compounds, while high levels of acetylated derivatives were recorded in Prokupac. Data reported in this study represent a certain contribution to a database of mechanical properties and chemical composition of grape varieties originating from Balkan.",
journal = "Chemistry & Biodiversity",
title = "Varietal differences of Prokupac, Evita and Čokot Zemun based on their anthocyanins content in grape skin extract",
doi = "10.1002/cbdv.202000858"
}

Živković, J., Jadranin, M., Przić, Z., Marković, N., Sokolović, D., Savikin, K.,& Menković, N.. (2021). Varietal differences of Prokupac, Evita and Čokot Zemun based on their anthocyanins content in grape skin extract. in Chemistry & Biodiversity.
https://doi.org/10.1002/cbdv.202000858

Živković J, Jadranin M, Przić Z, Marković N, Sokolović D, Savikin K, Menković N. Varietal differences of Prokupac, Evita and Čokot Zemun based on their anthocyanins content in grape skin extract. in Chemistry & Biodiversity. 2021;.
doi:10.1002/cbdv.202000858 .

Živković, Jelena, Jadranin, Milka, Przić, Zoran, Marković, Nebojša, Sokolović, Danijel, Savikin, Katarina, Menković, Nebojša, "Varietal differences of Prokupac, Evita and Čokot Zemun based on their anthocyanins content in grape skin extract" in Chemistry & Biodiversity (2021),
https://doi.org/10.1002/cbdv.202000858 . .

TY  - JOUR
AU  - Mudrić, Jelena
AU  - Šavikin, Katarina
AU  - Đekić, Ljiljana
AU  - Pavlović, Stefan
AU  - Kurćubić, Ivana
AU  - Ibrić, Svetlana
AU  - Đuriš, Jelena
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4891
AB  - Gentian (Gentiana lutea L., Gentianaceae) root extract (GRE) is used for the treatment of gastrointestinal disorders. However, its bioactive potential is limited in conventional forms due to the low bioavailability and short elimination half-life of the dominant bioactive compound, gentiopicroside. The aim of study was to encapsulate GRE in the lipid-based gastroretentive delivery system that could provide high yield and encapsulation efficiency, as well as the biphasic release of gentiopicroside from the tablets obtained by direct compression. Solid lipid microparticles (SLM) loaded with GRE were prepared by freeze-drying double (W/O/W) emulsions, which were obtained by a multiple emulsion–melt dispersion technique, with GRE as the inner water phase, Gelucire® 39/01 or 43/01, as lipid components, with or without the addition of porous silica (Sylysia® 350) in the outer water phase. Formulated SLM powders were examined by SEM and mercury intrusion porosimetry, as well as by determination of yield, encapsulation efficiency, and flow properties. Furthermore, in vitro dissolution of gentiopicroside, the size of the dispersed systems, mechanical properties, and mucoadhesion of tablets obtained by direct compression were investigated. The results have revealed that SLM with the macroporous structure were formulated, and, consequently, the powders floated immediately in the acidic medium. Formulation with porous silica (Sylysia® 350) and Gelucire® 43/01 as a solid lipid was characterized with the high yield end encapsulation efficiency. Furthermore, the mucoadhesive properties of tablets obtained by direct compression of that formulation, as well as the biphasic release of gentiopicroside, presence of nanoassociates in dissolution medium, and optimal mechanical properties indicated that a promising lipid-based gastroretentive system for GRE was developed.
PB  - MDPI
T2  - Pharmaceutics
T1  - Development of Lipid-Based Gastroretentive Delivery System for Gentian Extract by Double Emulsion-Melt Dispersion Technique
VL  - 13
IS  - 12
SP  - 2095
DO  - 10.3390/pharmaceutics13122095
ER  - 

@article{
author = "Mudrić, Jelena and Šavikin, Katarina and Đekić, Ljiljana and Pavlović, Stefan and Kurćubić, Ivana and Ibrić, Svetlana and Đuriš, Jelena",
year = "2021",
abstract = "Gentian (Gentiana lutea L., Gentianaceae) root extract (GRE) is used for the treatment of gastrointestinal disorders. However, its bioactive potential is limited in conventional forms due to the low bioavailability and short elimination half-life of the dominant bioactive compound, gentiopicroside. The aim of study was to encapsulate GRE in the lipid-based gastroretentive delivery system that could provide high yield and encapsulation efficiency, as well as the biphasic release of gentiopicroside from the tablets obtained by direct compression. Solid lipid microparticles (SLM) loaded with GRE were prepared by freeze-drying double (W/O/W) emulsions, which were obtained by a multiple emulsion–melt dispersion technique, with GRE as the inner water phase, Gelucire® 39/01 or 43/01, as lipid components, with or without the addition of porous silica (Sylysia® 350) in the outer water phase. Formulated SLM powders were examined by SEM and mercury intrusion porosimetry, as well as by determination of yield, encapsulation efficiency, and flow properties. Furthermore, in vitro dissolution of gentiopicroside, the size of the dispersed systems, mechanical properties, and mucoadhesion of tablets obtained by direct compression were investigated. The results have revealed that SLM with the macroporous structure were formulated, and, consequently, the powders floated immediately in the acidic medium. Formulation with porous silica (Sylysia® 350) and Gelucire® 43/01 as a solid lipid was characterized with the high yield end encapsulation efficiency. Furthermore, the mucoadhesive properties of tablets obtained by direct compression of that formulation, as well as the biphasic release of gentiopicroside, presence of nanoassociates in dissolution medium, and optimal mechanical properties indicated that a promising lipid-based gastroretentive system for GRE was developed.",
publisher = "MDPI",
journal = "Pharmaceutics",
title = "Development of Lipid-Based Gastroretentive Delivery System for Gentian Extract by Double Emulsion-Melt Dispersion Technique",
volume = "13",
number = "12",
pages = "2095",
doi = "10.3390/pharmaceutics13122095"
}

Mudrić, J., Šavikin, K., Đekić, L., Pavlović, S., Kurćubić, I., Ibrić, S.,& Đuriš, J.. (2021). Development of Lipid-Based Gastroretentive Delivery System for Gentian Extract by Double Emulsion-Melt Dispersion Technique. in Pharmaceutics
MDPI., 13(12), 2095.
https://doi.org/10.3390/pharmaceutics13122095

Mudrić J, Šavikin K, Đekić L, Pavlović S, Kurćubić I, Ibrić S, Đuriš J. Development of Lipid-Based Gastroretentive Delivery System for Gentian Extract by Double Emulsion-Melt Dispersion Technique. in Pharmaceutics. 2021;13(12):2095.
doi:10.3390/pharmaceutics13122095 .

Mudrić, Jelena, Šavikin, Katarina, Đekić, Ljiljana, Pavlović, Stefan, Kurćubić, Ivana, Ibrić, Svetlana, Đuriš, Jelena, "Development of Lipid-Based Gastroretentive Delivery System for Gentian Extract by Double Emulsion-Melt Dispersion Technique" in Pharmaceutics, 13, no. 12 (2021):2095,
https://doi.org/10.3390/pharmaceutics13122095 . .