Molecular characterization of bacteria from genera Bacillus and Pseudomonas as potential agents for biological control

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Molecular characterization of bacteria from genera Bacillus and Pseudomonas as potential agents for biological control (en)
Молекуларна карактеризација бактерија из родова Bacillus и Pseudomonas као потенцијалних агенаса за биолошку контролу (sr)
Molekularna karakterizacija bakterija iz rodova Bacillus i Pseudomonas kao potencijalnih agenasa za biološku kontrolu (sr_RS)
Authors

Publications

Phyllosphere fungal communities of plum and antifungal activity of indigenous phenazine-producing Pseudomonas synxantha against Monilinia laxa

Janakiev, Tamara; Dimkić, Ivica; Unković, Nikola; Ljaljević Grbić, Milica; Opsenica, Dejan; Gašić, Uroš; Stanković, Slaviša; Berić, Tanja

(Frontiers, 2019)

TY  - JOUR
AU  - Janakiev, Tamara
AU  - Dimkić, Ivica
AU  - Unković, Nikola
AU  - Ljaljević Grbić, Milica
AU  - Opsenica, Dejan
AU  - Gašić, Uroš
AU  - Stanković, Slaviša
AU  - Berić, Tanja
PY  - 2019
UR  - https://www.frontiersin.org/articles/10.3389/fmicb.2019.02287/abstract
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3093
AB  - European plum (Prunus domestica L.) is a significant commercial crop in Serbia in terms of total fruit production, and it is traditionally processed into slivovitz brandy. The annual plum yields in Serbia is strongly affected by Monilinia laxa, causing brown rot in stone fruits. The fungal communities associated with leaves and fruits of four local Serbian plum cultivars (Požegača, Ranka, Lepotica and Rodna) were investigated in two phenological stages during early (May) and late (July) fruit maturation. Alpha diversity indices showed that fungal communities were heterogeneous and Beta diversity indicated that autochthonous fungal communities depended upon seasonal changes and the cultivars themselves. The phylum Ascomycota was the most abundant in all samples, with relative abundance (RA) between 46% in the Požegača cultivar (May) and 89% in the Lepotica cultivar (July). The most abundant genus for all plum cultivars in May was Aureobasidium, with RA from 19.27 to 33.69%, followed by Cryptococcus, with 4.8 to 48.80%. In July, besides Cryptococcus, different genera (Metschnikowia, Fusarium and Hanseniaspora) were dominant on particular cultivars. Among all cultivable fungi, molecular identification of 11 M. laxa isolates from four plum cultivars was performed simultaneously. Bacterial isolates from the plum phyllosphere were tested for their potential antifungal activity against indigenous M. laxa isolates. The most potent antagonist, P4/16_1 was identified as Pseudomonas synxantha, which reduced mycelial growth of M. laxa from 80 to 87.5%. The production of volatile organic compounds was observed as well. Screening for the antibiotic coding gene was positive for phenazine-1-carboxylic acid, and benzene extraction of P. synxantha-active compounds was done. The crude benzene extract exhibited 57–63% inhibition of mycelial growth. The conducted LC/MS analysis of the crude extract confirmed the presence of phenazine derivatives amongst other compounds. Scanning electron microscopy revealed morpho-physiological changes in the hyphae of M. laxa isolates caused by the cell culture and the P. synxantha P4/16_1 crude benzene extract. This is first report of antagonistic activity of P. synxantha against M. laxa induced by diffusible and volatile antifungal compounds, and it appears to be a promising candidate for use as a biocontrol agent against brown rot-causing fungi.
PB  - Frontiers
T2  - Frontiers in Microbiology
T1  - Phyllosphere fungal communities of plum and antifungal activity of indigenous phenazine-producing Pseudomonas synxantha against Monilinia laxa
VL  - 10
SP  - 2287
DO  - 10.3389/fmicb.2019.02287
ER  - 
@article{
author = "Janakiev, Tamara and Dimkić, Ivica and Unković, Nikola and Ljaljević Grbić, Milica and Opsenica, Dejan and Gašić, Uroš and Stanković, Slaviša and Berić, Tanja",
year = "2019",
abstract = "European plum (Prunus domestica L.) is a significant commercial crop in Serbia in terms of total fruit production, and it is traditionally processed into slivovitz brandy. The annual plum yields in Serbia is strongly affected by Monilinia laxa, causing brown rot in stone fruits. The fungal communities associated with leaves and fruits of four local Serbian plum cultivars (Požegača, Ranka, Lepotica and Rodna) were investigated in two phenological stages during early (May) and late (July) fruit maturation. Alpha diversity indices showed that fungal communities were heterogeneous and Beta diversity indicated that autochthonous fungal communities depended upon seasonal changes and the cultivars themselves. The phylum Ascomycota was the most abundant in all samples, with relative abundance (RA) between 46% in the Požegača cultivar (May) and 89% in the Lepotica cultivar (July). The most abundant genus for all plum cultivars in May was Aureobasidium, with RA from 19.27 to 33.69%, followed by Cryptococcus, with 4.8 to 48.80%. In July, besides Cryptococcus, different genera (Metschnikowia, Fusarium and Hanseniaspora) were dominant on particular cultivars. Among all cultivable fungi, molecular identification of 11 M. laxa isolates from four plum cultivars was performed simultaneously. Bacterial isolates from the plum phyllosphere were tested for their potential antifungal activity against indigenous M. laxa isolates. The most potent antagonist, P4/16_1 was identified as Pseudomonas synxantha, which reduced mycelial growth of M. laxa from 80 to 87.5%. The production of volatile organic compounds was observed as well. Screening for the antibiotic coding gene was positive for phenazine-1-carboxylic acid, and benzene extraction of P. synxantha-active compounds was done. The crude benzene extract exhibited 57–63% inhibition of mycelial growth. The conducted LC/MS analysis of the crude extract confirmed the presence of phenazine derivatives amongst other compounds. Scanning electron microscopy revealed morpho-physiological changes in the hyphae of M. laxa isolates caused by the cell culture and the P. synxantha P4/16_1 crude benzene extract. This is first report of antagonistic activity of P. synxantha against M. laxa induced by diffusible and volatile antifungal compounds, and it appears to be a promising candidate for use as a biocontrol agent against brown rot-causing fungi.",
publisher = "Frontiers",
journal = "Frontiers in Microbiology",
title = "Phyllosphere fungal communities of plum and antifungal activity of indigenous phenazine-producing Pseudomonas synxantha against Monilinia laxa",
volume = "10",
pages = "2287",
doi = "10.3389/fmicb.2019.02287"
}
Janakiev, T., Dimkić, I., Unković, N., Ljaljević Grbić, M., Opsenica, D., Gašić, U., Stanković, S.,& Berić, T.. (2019). Phyllosphere fungal communities of plum and antifungal activity of indigenous phenazine-producing Pseudomonas synxantha against Monilinia laxa. in Frontiers in Microbiology
Frontiers., 10, 2287.
https://doi.org/10.3389/fmicb.2019.02287
Janakiev T, Dimkić I, Unković N, Ljaljević Grbić M, Opsenica D, Gašić U, Stanković S, Berić T. Phyllosphere fungal communities of plum and antifungal activity of indigenous phenazine-producing Pseudomonas synxantha against Monilinia laxa. in Frontiers in Microbiology. 2019;10:2287.
doi:10.3389/fmicb.2019.02287 .
Janakiev, Tamara, Dimkić, Ivica, Unković, Nikola, Ljaljević Grbić, Milica, Opsenica, Dejan, Gašić, Uroš, Stanković, Slaviša, Berić, Tanja, "Phyllosphere fungal communities of plum and antifungal activity of indigenous phenazine-producing Pseudomonas synxantha against Monilinia laxa" in Frontiers in Microbiology, 10 (2019):2287,
https://doi.org/10.3389/fmicb.2019.02287 . .
10
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Chemical composition and inhibitory activity of selected essential oils against fungi isolated from medicinal plants

Stević, Tatjana; Stanković, Slaviša; Šavikin, Katarina; Gođevac, Dejan; Dimkić, Ivica; Soković, Marina; Berić, Tanja

(Institute for Medicinal Plants Research "Dr. Josif Pancic", 2014)

TY  - JOUR
AU  - Stević, Tatjana
AU  - Stanković, Slaviša
AU  - Šavikin, Katarina
AU  - Gođevac, Dejan
AU  - Dimkić, Ivica
AU  - Soković, Marina
AU  - Berić, Tanja
PY  - 2014
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1454
AB  - In recent years great attention was paid to biological control, the application of natural products in order to protect crops and medicinal plants against contamination with phytopathogenic and saprophytic fungi. Essential oils have been evaluated as a potentially safe replacement for chemicals used for that purpose. In this regard, antifungal potential of six essential oils was examined coupled with determination of the complete qualitative and quantitative chemical composition by GC-FID and GC-MS analysis. While essential oils of rosemary, sage, Spanish sage and black pepper were exclusively composed of monoterpenes, vetiver essential oil was entirely composed of sesquiterpenes. In essential oil of cinnamon dominates (E)-cinnamaldehyde. Applying the in vitro microdilution method, it was found that all essential oils were active in inhibiting the growth of all tested 21 pre- and post-harvest phytopathogenic and saprophytic fungi. MIC and MFC ranged from 1.2 mg ml-1 up to 22.6 mg ml-1 according to the test oil. Rosemary oil showed the best antifungal potential, followed by black pepper and cinnamon oil. Sage and Spanish sage oils also exhibited significant antifungal potential. Vetiver oil demonstrated the lowest antifungal activity. Essential oils that showed considerable antifungal potential are good candidates for further examination of their use in preventing and/or protection of medicinal plants, their seeds and dried drugs against fungal infections, both in the field and in warehouses.
AB  - Poslednjih godina velika pažnja poklanja se biološkoj kontroli to jest primeni prirodnih produkata u zaštiti, kako poljoprivrednih kultura, tako i lekovitog bilja od kontaminacije fitopatogenim i saprofitnim gljivama. U tom smislu, ispitivan je antifungalni potencijal šest etarskih ulja uz određivanje kompletnog kvalitativnog i kvantitativnog hemijskog sastava primenom GC-FID i GC-MS analiza. Dok se korišćeni uzorci etarskih ulja ruzmarina, žalfije, španske žalfije i crnog bibera isključivo sastoje od monoterpena, u ulju vetivera potpuno dominiraju seskviterpeni. U etarskom ulju cimeta najzastupljenija komponneta je bila (E)- cinemaldehid. Primenom in vitro mikrodilucione metode, utvrđeno je da su sva ulja bila aktivna u inhibiciji rasta 21 testirane, pre- i post-žetvene, fitopatogene i saprofitne gljive, sa razlikama u efikasnosti. MIC i MFC vrednosti su varirale u opsegu od 1,2 mg ml-1 do 22,6 mg ml-1, u zavisnosti od ispitivanog ulja. Najbolja antifungalna aktivnost utvrđena je za ulje ruzmarina, nešto slabija za ulja crnog bibera i cimeta, dok je ulje vetivera ispoljilo najslabiju aktivnost. Etarska ulja obe vrste žalfija ispoljila su značajan antifungalni potencijal. Etarska ulja koja poseduju značajan antifungalni potencijal su dobri kandidati za nastavak istraživanja u pravcu njihove primene u prevenciji i zaštiti useva lekovitog bilja, kao i njihovih semena i osušenih biljnih droga od gljivičnih infekcija, i u polju i u skladištima.
PB  - Institute for Medicinal Plants Research "Dr. Josif Pancic"
T2  - Lekovite sirovine
T1  - Chemical composition and inhibitory activity of selected essential oils against fungi isolated from medicinal plants
T1  - Hemijski sastav i inhibitorna aktivnost odabranih etarskih ulja na gljive izolovane sa lekovitog bilja
VL  - 34
IS  - 34
SP  - 69
EP  - 80
DO  - 10.5937/leksir1434069S
ER  - 
@article{
author = "Stević, Tatjana and Stanković, Slaviša and Šavikin, Katarina and Gođevac, Dejan and Dimkić, Ivica and Soković, Marina and Berić, Tanja",
year = "2014",
abstract = "In recent years great attention was paid to biological control, the application of natural products in order to protect crops and medicinal plants against contamination with phytopathogenic and saprophytic fungi. Essential oils have been evaluated as a potentially safe replacement for chemicals used for that purpose. In this regard, antifungal potential of six essential oils was examined coupled with determination of the complete qualitative and quantitative chemical composition by GC-FID and GC-MS analysis. While essential oils of rosemary, sage, Spanish sage and black pepper were exclusively composed of monoterpenes, vetiver essential oil was entirely composed of sesquiterpenes. In essential oil of cinnamon dominates (E)-cinnamaldehyde. Applying the in vitro microdilution method, it was found that all essential oils were active in inhibiting the growth of all tested 21 pre- and post-harvest phytopathogenic and saprophytic fungi. MIC and MFC ranged from 1.2 mg ml-1 up to 22.6 mg ml-1 according to the test oil. Rosemary oil showed the best antifungal potential, followed by black pepper and cinnamon oil. Sage and Spanish sage oils also exhibited significant antifungal potential. Vetiver oil demonstrated the lowest antifungal activity. Essential oils that showed considerable antifungal potential are good candidates for further examination of their use in preventing and/or protection of medicinal plants, their seeds and dried drugs against fungal infections, both in the field and in warehouses., Poslednjih godina velika pažnja poklanja se biološkoj kontroli to jest primeni prirodnih produkata u zaštiti, kako poljoprivrednih kultura, tako i lekovitog bilja od kontaminacije fitopatogenim i saprofitnim gljivama. U tom smislu, ispitivan je antifungalni potencijal šest etarskih ulja uz određivanje kompletnog kvalitativnog i kvantitativnog hemijskog sastava primenom GC-FID i GC-MS analiza. Dok se korišćeni uzorci etarskih ulja ruzmarina, žalfije, španske žalfije i crnog bibera isključivo sastoje od monoterpena, u ulju vetivera potpuno dominiraju seskviterpeni. U etarskom ulju cimeta najzastupljenija komponneta je bila (E)- cinemaldehid. Primenom in vitro mikrodilucione metode, utvrđeno je da su sva ulja bila aktivna u inhibiciji rasta 21 testirane, pre- i post-žetvene, fitopatogene i saprofitne gljive, sa razlikama u efikasnosti. MIC i MFC vrednosti su varirale u opsegu od 1,2 mg ml-1 do 22,6 mg ml-1, u zavisnosti od ispitivanog ulja. Najbolja antifungalna aktivnost utvrđena je za ulje ruzmarina, nešto slabija za ulja crnog bibera i cimeta, dok je ulje vetivera ispoljilo najslabiju aktivnost. Etarska ulja obe vrste žalfija ispoljila su značajan antifungalni potencijal. Etarska ulja koja poseduju značajan antifungalni potencijal su dobri kandidati za nastavak istraživanja u pravcu njihove primene u prevenciji i zaštiti useva lekovitog bilja, kao i njihovih semena i osušenih biljnih droga od gljivičnih infekcija, i u polju i u skladištima.",
publisher = "Institute for Medicinal Plants Research "Dr. Josif Pancic"",
journal = "Lekovite sirovine",
title = "Chemical composition and inhibitory activity of selected essential oils against fungi isolated from medicinal plants, Hemijski sastav i inhibitorna aktivnost odabranih etarskih ulja na gljive izolovane sa lekovitog bilja",
volume = "34",
number = "34",
pages = "69-80",
doi = "10.5937/leksir1434069S"
}
Stević, T., Stanković, S., Šavikin, K., Gođevac, D., Dimkić, I., Soković, M.,& Berić, T.. (2014). Chemical composition and inhibitory activity of selected essential oils against fungi isolated from medicinal plants. in Lekovite sirovine
Institute for Medicinal Plants Research "Dr. Josif Pancic"., 34(34), 69-80.
https://doi.org/10.5937/leksir1434069S
Stević T, Stanković S, Šavikin K, Gođevac D, Dimkić I, Soković M, Berić T. Chemical composition and inhibitory activity of selected essential oils against fungi isolated from medicinal plants. in Lekovite sirovine. 2014;34(34):69-80.
doi:10.5937/leksir1434069S .
Stević, Tatjana, Stanković, Slaviša, Šavikin, Katarina, Gođevac, Dejan, Dimkić, Ivica, Soković, Marina, Berić, Tanja, "Chemical composition and inhibitory activity of selected essential oils against fungi isolated from medicinal plants" in Lekovite sirovine, 34, no. 34 (2014):69-80,
https://doi.org/10.5937/leksir1434069S . .
3
2

Antifungal activity of selected essential oils against fungi isolated from medicinal plant

Stevic, Tatjana; Beric, Tanja; Savikin, Katarina; Soković, Marina; Gođevac, Dejan; Dimkic, Ivica; Stankovic, Slavisa

(Elsevier, 2014)

TY  - JOUR
AU  - Stevic, Tatjana
AU  - Beric, Tanja
AU  - Savikin, Katarina
AU  - Soković, Marina
AU  - Gođevac, Dejan
AU  - Dimkic, Ivica
AU  - Stankovic, Slavisa
PY  - 2014
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1575
AB  - The development of protective products with natural origin as alternatives to synthetic fungicides is currently in the spotlight. Qualitative and quantitative chemical analysis of 16 selected essential oils was determined. Then, antifungal properties determined by in vitro microdilution method against 21 fungi isolated from herbal drugs were evaluated. All tested oils showed some antifungal activity against all fungi used. Savory, thyme and oregano oils, characterized by the presence of phenol such as carvacrol and thymol, and rose oil containing mainly monoterpene alcohols (citronellol and geraniol) proved to be the most effective inhibitor of all fungi tested. Also, combination of particular oils showed reduction of the MIC values when combined, commendatory mixtures for potential application in practice. Moreover, the reduction of the total number of fungi, in situ, using selected essential oils was determined.
PB  - Elsevier
T2  - Industrial Crops and Products
T1  - Antifungal activity of selected essential oils against fungi isolated from medicinal plant
VL  - 55
SP  - 116
EP  - 122
DO  - 10.1016/j.indcrop.2014.02.011
ER  - 
@article{
author = "Stevic, Tatjana and Beric, Tanja and Savikin, Katarina and Soković, Marina and Gođevac, Dejan and Dimkic, Ivica and Stankovic, Slavisa",
year = "2014",
abstract = "The development of protective products with natural origin as alternatives to synthetic fungicides is currently in the spotlight. Qualitative and quantitative chemical analysis of 16 selected essential oils was determined. Then, antifungal properties determined by in vitro microdilution method against 21 fungi isolated from herbal drugs were evaluated. All tested oils showed some antifungal activity against all fungi used. Savory, thyme and oregano oils, characterized by the presence of phenol such as carvacrol and thymol, and rose oil containing mainly monoterpene alcohols (citronellol and geraniol) proved to be the most effective inhibitor of all fungi tested. Also, combination of particular oils showed reduction of the MIC values when combined, commendatory mixtures for potential application in practice. Moreover, the reduction of the total number of fungi, in situ, using selected essential oils was determined.",
publisher = "Elsevier",
journal = "Industrial Crops and Products",
title = "Antifungal activity of selected essential oils against fungi isolated from medicinal plant",
volume = "55",
pages = "116-122",
doi = "10.1016/j.indcrop.2014.02.011"
}
Stevic, T., Beric, T., Savikin, K., Soković, M., Gođevac, D., Dimkic, I.,& Stankovic, S.. (2014). Antifungal activity of selected essential oils against fungi isolated from medicinal plant. in Industrial Crops and Products
Elsevier., 55, 116-122.
https://doi.org/10.1016/j.indcrop.2014.02.011
Stevic T, Beric T, Savikin K, Soković M, Gođevac D, Dimkic I, Stankovic S. Antifungal activity of selected essential oils against fungi isolated from medicinal plant. in Industrial Crops and Products. 2014;55:116-122.
doi:10.1016/j.indcrop.2014.02.011 .
Stevic, Tatjana, Beric, Tanja, Savikin, Katarina, Soković, Marina, Gođevac, Dejan, Dimkic, Ivica, Stankovic, Slavisa, "Antifungal activity of selected essential oils against fungi isolated from medicinal plant" in Industrial Crops and Products, 55 (2014):116-122,
https://doi.org/10.1016/j.indcrop.2014.02.011 . .
136
89
150

Characterisation of New Bacillus circulans Strain Isolated from Oil Shale

Dragutinović, Vesna; Vrvić, Miroslav; Swiecicka, Izabela; Cvetković, Olga; Beric, Tanja; Stankovic, Slavisa

(Croatia : University of Zagreb, Faculty of Food Technology and Biotechnology, 2012)

TY  - JOUR
AU  - Dragutinović, Vesna
AU  - Vrvić, Miroslav
AU  - Swiecicka, Izabela
AU  - Cvetković, Olga
AU  - Beric, Tanja
AU  - Stankovic, Slavisa
PY  - 2012
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1017
AB  - A new strain of Bacillus sp. was obtained during experiments of oil shale demineralization, which were carried out in order to get 'pure' organic matter (kerogen). The demineralization efficiency of newly isolated strain was found to be substantially higher in comparison with that of Bacillus circulans Jordan 1890. On the basis of the biochemical characteristics, protein patterns and fatty acid composition, as well as the whole genome profile and 16S rDNA sequencing, the new strain was identified as Bacillus circulans VD01.
PB  - Croatia : University of Zagreb, Faculty of Food Technology and Biotechnology
T2  - Food Technology and Biotechnology
T1  - Characterisation of New Bacillus circulans Strain Isolated from Oil Shale
VL  - 50
IS  - 1
SP  - 123
EP  - 127
UR  - https://hdl.handle.net/21.15107/rcub_cer_1017
ER  - 
@article{
author = "Dragutinović, Vesna and Vrvić, Miroslav and Swiecicka, Izabela and Cvetković, Olga and Beric, Tanja and Stankovic, Slavisa",
year = "2012",
abstract = "A new strain of Bacillus sp. was obtained during experiments of oil shale demineralization, which were carried out in order to get 'pure' organic matter (kerogen). The demineralization efficiency of newly isolated strain was found to be substantially higher in comparison with that of Bacillus circulans Jordan 1890. On the basis of the biochemical characteristics, protein patterns and fatty acid composition, as well as the whole genome profile and 16S rDNA sequencing, the new strain was identified as Bacillus circulans VD01.",
publisher = "Croatia : University of Zagreb, Faculty of Food Technology and Biotechnology",
journal = "Food Technology and Biotechnology",
title = "Characterisation of New Bacillus circulans Strain Isolated from Oil Shale",
volume = "50",
number = "1",
pages = "123-127",
url = "https://hdl.handle.net/21.15107/rcub_cer_1017"
}
Dragutinović, V., Vrvić, M., Swiecicka, I., Cvetković, O., Beric, T.,& Stankovic, S.. (2012). Characterisation of New Bacillus circulans Strain Isolated from Oil Shale. in Food Technology and Biotechnology
Croatia : University of Zagreb, Faculty of Food Technology and Biotechnology., 50(1), 123-127.
https://hdl.handle.net/21.15107/rcub_cer_1017
Dragutinović V, Vrvić M, Swiecicka I, Cvetković O, Beric T, Stankovic S. Characterisation of New Bacillus circulans Strain Isolated from Oil Shale. in Food Technology and Biotechnology. 2012;50(1):123-127.
https://hdl.handle.net/21.15107/rcub_cer_1017 .
Dragutinović, Vesna, Vrvić, Miroslav, Swiecicka, Izabela, Cvetković, Olga, Beric, Tanja, Stankovic, Slavisa, "Characterisation of New Bacillus circulans Strain Isolated from Oil Shale" in Food Technology and Biotechnology, 50, no. 1 (2012):123-127,
https://hdl.handle.net/21.15107/rcub_cer_1017 .
2
2