TY  - JOUR
AU  - Bajić, Vladan
AU  - Misić, Nataša
AU  - Stanković, Ivana
AU  - Zarić, Božidarka
AU  - Perry, George
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4773
AB  - Does Alzheimer Disease show a decline in cognitive functions that relate to the awareness of external reality? In this paper, we will propose a perspective that patients with increasing symptoms of AD show a change in the awareness of subjective versus objective representative axis of reality thus consequently move to a more internal like perception of reality. This paradigm shift suggests that new insights into the dynamicity of the conscious representation of reality in the AD brain may give us new clues to the very early signs of memory and self-awareness impairment that originates from, in our view the microtubules. Dialog between Adso and William, in Umberto Eco’s The Name of the Rose, Third Day: Vespers. “But how does it happen,” I said with admiration, “that you were able to solve the mystery of the library looking at it from the outside, and you were unable to solve it when you were inside?” “Thus, God knows the world, because He conceived it in His mind, as if it was from the outside, before it was created, and we do not know its rule, because we live inside it, having found it already made.
PB  - SAGE Publications Ltd
T2  - Neuroscience Insights
T1  - Alzheimer’s and Consciousness: How Much Subjectivity Is Objective?
VL  - 16
DO  - 10.1177/26331055211033869
ER  - 

@article{
author = "Bajić, Vladan and Misić, Nataša and Stanković, Ivana and Zarić, Božidarka and Perry, George",
year = "2021",
abstract = "Does Alzheimer Disease show a decline in cognitive functions that relate to the awareness of external reality? In this paper, we will propose a perspective that patients with increasing symptoms of AD show a change in the awareness of subjective versus objective representative axis of reality thus consequently move to a more internal like perception of reality. This paradigm shift suggests that new insights into the dynamicity of the conscious representation of reality in the AD brain may give us new clues to the very early signs of memory and self-awareness impairment that originates from, in our view the microtubules. Dialog between Adso and William, in Umberto Eco’s The Name of the Rose, Third Day: Vespers. “But how does it happen,” I said with admiration, “that you were able to solve the mystery of the library looking at it from the outside, and you were unable to solve it when you were inside?” “Thus, God knows the world, because He conceived it in His mind, as if it was from the outside, before it was created, and we do not know its rule, because we live inside it, having found it already made.",
publisher = "SAGE Publications Ltd",
journal = "Neuroscience Insights",
title = "Alzheimer’s and Consciousness: How Much Subjectivity Is Objective?",
volume = "16",
doi = "10.1177/26331055211033869"
}

Bajić, V., Misić, N., Stanković, I., Zarić, B.,& Perry, G.. (2021). Alzheimer’s and Consciousness: How Much Subjectivity Is Objective?. in Neuroscience Insights
SAGE Publications Ltd., 16.
https://doi.org/10.1177/26331055211033869

Bajić V, Misić N, Stanković I, Zarić B, Perry G. Alzheimer’s and Consciousness: How Much Subjectivity Is Objective?. in Neuroscience Insights. 2021;16.
doi:10.1177/26331055211033869 .

Bajić, Vladan, Misić, Nataša, Stanković, Ivana, Zarić, Božidarka, Perry, George, "Alzheimer’s and Consciousness: How Much Subjectivity Is Objective?" in Neuroscience Insights, 16 (2021),
https://doi.org/10.1177/26331055211033869 . .

TY  - JOUR
AU  - Nikolić, Ines
AU  - Lunter, Dominique Jasmin
AU  - Randjelović, Danijela
AU  - Žugić, Ana
AU  - Tadić, Vanja
AU  - Marković, Bojan D.
AU  - Cekic, Nebojsa
AU  - Živković, Lada
AU  - Topalovic, Dijana
AU  - Spremo-Potparević, Biljana
AU  - Daniels, Rolf
AU  - Savić, Snežana D.
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3719
AB  - The objective of this work was to investigate and profoundly characterize low-energy nanoemulsions as multifunctional carriers, with slight reference to dermal administration. An evidence-based approach was offered for deepening the knowledge on their formation via spontaneous emulsification. Curcumin, a compound of natural origin, potentially powerful therapeutic, was chosen as a model API. Due to curcumin's demanding properties (instability, poor solubility, low permeability), its potentials remain unreached. Low-energy nanoemulsions were considered carriers capable of overcoming imposed obstacles. Formulation consisting of Polysorbate 80 and soybean lecithin as stabilizers (9:1, 10%), medium-chain triglycerides as the oil phase (10%) and ultrapure water was selected for curcumin incorporation in 3 different concentrations (1, 2 and 3 mg/mL). Physicochemical stability was demonstrated during 3 months of monitoring (mean droplet size: 111.3-146.8 nm; PDI  LT  0.2; pH: 4.73-5.73). Curcumin's release from developed vehicles followed Higuchi's kinetics. DPPH (IC50 = 0.1187 mg/ mL) and FRAP (1.19 +/- 0.02 mmol/g) assays confirmed that curcumin acts as a potent antioxidant through different mechanisms, with no alterations after incorporation in the formulation. High biocompatibility in line with antigenotoxic activity of curcumin-loaded formulations (protective and reparative) was estimated through Comet assay. A multidisciplinary approach is needed to fully characterize developed systems, directing them to more concrete application possibilities.
PB  - Elsevier
T2  - International Journal of Pharmaceutics
T1  - Curcumin-loaded low-energy nanoemulsions as a prototype of multifunctional vehicles for different administration routes: Physicochemical and in vitro peculiarities important for dermal application
VL  - 550
IS  - 1-2
SP  - 333
EP  - 346
DO  - 10.1016/j.ijpharm.2018.08.060
ER  - 

@article{
author = "Nikolić, Ines and Lunter, Dominique Jasmin and Randjelović, Danijela and Žugić, Ana and Tadić, Vanja and Marković, Bojan D. and Cekic, Nebojsa and Živković, Lada and Topalovic, Dijana and Spremo-Potparević, Biljana and Daniels, Rolf and Savić, Snežana D.",
year = "2018",
abstract = "The objective of this work was to investigate and profoundly characterize low-energy nanoemulsions as multifunctional carriers, with slight reference to dermal administration. An evidence-based approach was offered for deepening the knowledge on their formation via spontaneous emulsification. Curcumin, a compound of natural origin, potentially powerful therapeutic, was chosen as a model API. Due to curcumin's demanding properties (instability, poor solubility, low permeability), its potentials remain unreached. Low-energy nanoemulsions were considered carriers capable of overcoming imposed obstacles. Formulation consisting of Polysorbate 80 and soybean lecithin as stabilizers (9:1, 10%), medium-chain triglycerides as the oil phase (10%) and ultrapure water was selected for curcumin incorporation in 3 different concentrations (1, 2 and 3 mg/mL). Physicochemical stability was demonstrated during 3 months of monitoring (mean droplet size: 111.3-146.8 nm; PDI  LT  0.2; pH: 4.73-5.73). Curcumin's release from developed vehicles followed Higuchi's kinetics. DPPH (IC50 = 0.1187 mg/ mL) and FRAP (1.19 +/- 0.02 mmol/g) assays confirmed that curcumin acts as a potent antioxidant through different mechanisms, with no alterations after incorporation in the formulation. High biocompatibility in line with antigenotoxic activity of curcumin-loaded formulations (protective and reparative) was estimated through Comet assay. A multidisciplinary approach is needed to fully characterize developed systems, directing them to more concrete application possibilities.",
publisher = "Elsevier",
journal = "International Journal of Pharmaceutics",
title = "Curcumin-loaded low-energy nanoemulsions as a prototype of multifunctional vehicles for different administration routes: Physicochemical and in vitro peculiarities important for dermal application",
volume = "550",
number = "1-2",
pages = "333-346",
doi = "10.1016/j.ijpharm.2018.08.060"
}

Nikolić, I., Lunter, D. J., Randjelović, D., Žugić, A., Tadić, V., Marković, B. D., Cekic, N., Živković, L., Topalovic, D., Spremo-Potparević, B., Daniels, R.,& Savić, S. D.. (2018). Curcumin-loaded low-energy nanoemulsions as a prototype of multifunctional vehicles for different administration routes: Physicochemical and in vitro peculiarities important for dermal application. in International Journal of Pharmaceutics
Elsevier., 550(1-2), 333-346.
https://doi.org/10.1016/j.ijpharm.2018.08.060

Nikolić I, Lunter DJ, Randjelović D, Žugić A, Tadić V, Marković BD, Cekic N, Živković L, Topalovic D, Spremo-Potparević B, Daniels R, Savić SD. Curcumin-loaded low-energy nanoemulsions as a prototype of multifunctional vehicles for different administration routes: Physicochemical and in vitro peculiarities important for dermal application. in International Journal of Pharmaceutics. 2018;550(1-2):333-346.
doi:10.1016/j.ijpharm.2018.08.060 .

Nikolić, Ines, Lunter, Dominique Jasmin, Randjelović, Danijela, Žugić, Ana, Tadić, Vanja, Marković, Bojan D., Cekic, Nebojsa, Živković, Lada, Topalovic, Dijana, Spremo-Potparević, Biljana, Daniels, Rolf, Savić, Snežana D., "Curcumin-loaded low-energy nanoemulsions as a prototype of multifunctional vehicles for different administration routes: Physicochemical and in vitro peculiarities important for dermal application" in International Journal of Pharmaceutics, 550, no. 1-2 (2018):333-346,
https://doi.org/10.1016/j.ijpharm.2018.08.060 . .

TY  - JOUR
AU  - Nikolić, Ines
AU  - Lunter, Dominique Jasmin
AU  - Randjelović, Danijela
AU  - Žugić, Ana
AU  - Tadić, Vanja
AU  - Marković, Bojan D.
AU  - Cekic, Nebojsa
AU  - Živković, Lada
AU  - Topalovic, Dijana
AU  - Spremo-Potparević, Biljana
AU  - Daniels, Rolf
AU  - Savić, Snežana D.
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2425
AB  - The objective of this work was to investigate and profoundly characterize low-energy nanoemulsions as multifunctional carriers, with slight reference to dermal administration. An evidence-based approach was offered for deepening the knowledge on their formation via spontaneous emulsification. Curcumin, a compound of natural origin, potentially powerful therapeutic, was chosen as a model API. Due to curcumin's demanding properties (instability, poor solubility, low permeability), its potentials remain unreached. Low-energy nanoemulsions were considered carriers capable of overcoming imposed obstacles. Formulation consisting of Polysorbate 80 and soybean lecithin as stabilizers (9:1, 10%), medium-chain triglycerides as the oil phase (10%) and ultrapure water was selected for curcumin incorporation in 3 different concentrations (1, 2 and 3 mg/mL). Physicochemical stability was demonstrated during 3 months of monitoring (mean droplet size: 111.3-146.8 nm; PDI  LT  0.2; pH: 4.73-5.73). Curcumin's release from developed vehicles followed Higuchi's kinetics. DPPH (IC50 = 0.1187 mg/ mL) and FRAP (1.19 +/- 0.02 mmol/g) assays confirmed that curcumin acts as a potent antioxidant through different mechanisms, with no alterations after incorporation in the formulation. High biocompatibility in line with antigenotoxic activity of curcumin-loaded formulations (protective and reparative) was estimated through Comet assay. A multidisciplinary approach is needed to fully characterize developed systems, directing them to more concrete application possibilities.
PB  - Elsevier
T2  - International Journal of Pharmaceutics
T1  - Curcumin-loaded low-energy nanoemulsions as a prototype of multifunctional vehicles for different administration routes: Physicochemical and in vitro peculiarities important for dermal application
VL  - 550
IS  - 1-2
SP  - 333
EP  - 346
DO  - 10.1016/j.ijpharm.2018.08.060
ER  - 

@article{
author = "Nikolić, Ines and Lunter, Dominique Jasmin and Randjelović, Danijela and Žugić, Ana and Tadić, Vanja and Marković, Bojan D. and Cekic, Nebojsa and Živković, Lada and Topalovic, Dijana and Spremo-Potparević, Biljana and Daniels, Rolf and Savić, Snežana D.",
year = "2018",
abstract = "The objective of this work was to investigate and profoundly characterize low-energy nanoemulsions as multifunctional carriers, with slight reference to dermal administration. An evidence-based approach was offered for deepening the knowledge on their formation via spontaneous emulsification. Curcumin, a compound of natural origin, potentially powerful therapeutic, was chosen as a model API. Due to curcumin's demanding properties (instability, poor solubility, low permeability), its potentials remain unreached. Low-energy nanoemulsions were considered carriers capable of overcoming imposed obstacles. Formulation consisting of Polysorbate 80 and soybean lecithin as stabilizers (9:1, 10%), medium-chain triglycerides as the oil phase (10%) and ultrapure water was selected for curcumin incorporation in 3 different concentrations (1, 2 and 3 mg/mL). Physicochemical stability was demonstrated during 3 months of monitoring (mean droplet size: 111.3-146.8 nm; PDI  LT  0.2; pH: 4.73-5.73). Curcumin's release from developed vehicles followed Higuchi's kinetics. DPPH (IC50 = 0.1187 mg/ mL) and FRAP (1.19 +/- 0.02 mmol/g) assays confirmed that curcumin acts as a potent antioxidant through different mechanisms, with no alterations after incorporation in the formulation. High biocompatibility in line with antigenotoxic activity of curcumin-loaded formulations (protective and reparative) was estimated through Comet assay. A multidisciplinary approach is needed to fully characterize developed systems, directing them to more concrete application possibilities.",
publisher = "Elsevier",
journal = "International Journal of Pharmaceutics",
title = "Curcumin-loaded low-energy nanoemulsions as a prototype of multifunctional vehicles for different administration routes: Physicochemical and in vitro peculiarities important for dermal application",
volume = "550",
number = "1-2",
pages = "333-346",
doi = "10.1016/j.ijpharm.2018.08.060"
}

Nikolić, I., Lunter, D. J., Randjelović, D., Žugić, A., Tadić, V., Marković, B. D., Cekic, N., Živković, L., Topalovic, D., Spremo-Potparević, B., Daniels, R.,& Savić, S. D.. (2018). Curcumin-loaded low-energy nanoemulsions as a prototype of multifunctional vehicles for different administration routes: Physicochemical and in vitro peculiarities important for dermal application. in International Journal of Pharmaceutics
Elsevier., 550(1-2), 333-346.
https://doi.org/10.1016/j.ijpharm.2018.08.060

Nikolić I, Lunter DJ, Randjelović D, Žugić A, Tadić V, Marković BD, Cekic N, Živković L, Topalovic D, Spremo-Potparević B, Daniels R, Savić SD. Curcumin-loaded low-energy nanoemulsions as a prototype of multifunctional vehicles for different administration routes: Physicochemical and in vitro peculiarities important for dermal application. in International Journal of Pharmaceutics. 2018;550(1-2):333-346.
doi:10.1016/j.ijpharm.2018.08.060 .

Nikolić, Ines, Lunter, Dominique Jasmin, Randjelović, Danijela, Žugić, Ana, Tadić, Vanja, Marković, Bojan D., Cekic, Nebojsa, Živković, Lada, Topalovic, Dijana, Spremo-Potparević, Biljana, Daniels, Rolf, Savić, Snežana D., "Curcumin-loaded low-energy nanoemulsions as a prototype of multifunctional vehicles for different administration routes: Physicochemical and in vitro peculiarities important for dermal application" in International Journal of Pharmaceutics, 550, no. 1-2 (2018):333-346,
https://doi.org/10.1016/j.ijpharm.2018.08.060 . .

TY  - JOUR
AU  - Borozan, Sunčica
AU  - Zlatović, Mario
AU  - Stojanović, Srđan
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2007
AB  - We analyzed the potential influence of anion-pi interactions on the stability of complexes of proteins and halogen-containing non-natural amino acids. Anion-pi interactions are distance and orientation dependent and our ab initio calculations showed that their energy can be lower than -8 kcal mol(-1), while most of their interaction energies lie in the range from -1 to -4 kcal mol(-1). About 20 % of these interactions were found to be repulsive. We have observed that Tyr has the highest occurrence among the aromatic residues involved in anion-pi interactions, while His made the least contribution. Furthermore, our study showed that 67 % of total interactions in the dataset are multiple anion-pi interactions. Most of the amino acid residues involved in anion-pi interactions tend to be buried in the solvent-excluded environment. The majority of the anion-pi interacting residues are located in regions with helical secondary structure. Analysis of stabilization centers for these complexes showed that all of the six residues capable of anion-pi interactions are important in locating one or more of such centers. We found that anion-pi interacting residues are sometimes involved in simultaneous interactions with halogens as well. With all that in mind, we can conclude that the anion-pi interactions can show significant influence on molecular organization and on the structural stability of the complexes of proteins and halogen-containing non-natural amino acids. Their influence should not be neglected in supramolecular chemistry and crystal engineering fields as well.
PB  - Springer, New York
T2  - Journal of Biological Inorganic Chemistry
T1  - Anion-pi interactions in complexes of proteins and halogen-containing amino acids
VL  - 21
IS  - 3
SP  - 357
EP  - 368
DO  - 10.1007/s00775-016-1346-y
ER  - 

@article{
author = "Borozan, Sunčica and Zlatović, Mario and Stojanović, Srđan",
year = "2016",
abstract = "We analyzed the potential influence of anion-pi interactions on the stability of complexes of proteins and halogen-containing non-natural amino acids. Anion-pi interactions are distance and orientation dependent and our ab initio calculations showed that their energy can be lower than -8 kcal mol(-1), while most of their interaction energies lie in the range from -1 to -4 kcal mol(-1). About 20 % of these interactions were found to be repulsive. We have observed that Tyr has the highest occurrence among the aromatic residues involved in anion-pi interactions, while His made the least contribution. Furthermore, our study showed that 67 % of total interactions in the dataset are multiple anion-pi interactions. Most of the amino acid residues involved in anion-pi interactions tend to be buried in the solvent-excluded environment. The majority of the anion-pi interacting residues are located in regions with helical secondary structure. Analysis of stabilization centers for these complexes showed that all of the six residues capable of anion-pi interactions are important in locating one or more of such centers. We found that anion-pi interacting residues are sometimes involved in simultaneous interactions with halogens as well. With all that in mind, we can conclude that the anion-pi interactions can show significant influence on molecular organization and on the structural stability of the complexes of proteins and halogen-containing non-natural amino acids. Their influence should not be neglected in supramolecular chemistry and crystal engineering fields as well.",
publisher = "Springer, New York",
journal = "Journal of Biological Inorganic Chemistry",
title = "Anion-pi interactions in complexes of proteins and halogen-containing amino acids",
volume = "21",
number = "3",
pages = "357-368",
doi = "10.1007/s00775-016-1346-y"
}

Borozan, S., Zlatović, M.,& Stojanović, S.. (2016). Anion-pi interactions in complexes of proteins and halogen-containing amino acids. in Journal of Biological Inorganic Chemistry
Springer, New York., 21(3), 357-368.
https://doi.org/10.1007/s00775-016-1346-y

Borozan S, Zlatović M, Stojanović S. Anion-pi interactions in complexes of proteins and halogen-containing amino acids. in Journal of Biological Inorganic Chemistry. 2016;21(3):357-368.
doi:10.1007/s00775-016-1346-y .

Borozan, Sunčica, Zlatović, Mario, Stojanović, Srđan, "Anion-pi interactions in complexes of proteins and halogen-containing amino acids" in Journal of Biological Inorganic Chemistry, 21, no. 3 (2016):357-368,
https://doi.org/10.1007/s00775-016-1346-y . .

TY  - JOUR
AU  - Cabarkapa, Andrea
AU  - Borozan, Sunčica
AU  - Živković, Lada
AU  - Milanovic-Cabarkapa, Mirjana
AU  - Stojanović, Srđan
AU  - Bajic, Vladan
AU  - Spremo-Potparević, Biljana
PY  - 2015
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1706
AB  - The aim of this study was to determine oxidative alterations leading to cellular dysfunctions in Pb-exposed subjects by evaluating damage to all major classes of biomolecules in the cell, lipid peroxidation, protein and DNA damage and determine relationships between parameters of Pb toxicity and specific biomarkers of oxidative damage.Analysis was conducted of smelter workers with high blood Pb and urine aminolevulinic acid levels and slightly elevated values of coproporphyrin and erythrocyte protoporphyrin IX. Significant decreases of thiol groups and increases in carbonyl groups as protein degradation end products, and of nitrite were detected. Elevated rates of lipid peroxidation and rises in the activities of the antioxidant enzymes Cu-Zn superoxide dismutase and catalase were also observed. Both enzymes showed positive correlations with the blood lead levels and urine coproporphyrin, while thiol groups correlated negatively with the same indices. The genotoxic potential of lead was manifested through an increased number of DNA-damaged cells. Increased activities of serum lactate dehydrogenase isoenzymes indicated cellular damage in the lungs, kidneys, and liver. These lead-induced impairments should be taken into consideration in the assessment of Pb-related health hazards.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Toxicological and Environmental Chemistry
T1  - Implications of oxidative stress in occupational exposure to lead on a cellular level
VL  - 97
IS  - 6
SP  - 799
EP  - 813
DO  - 10.1080/02772248.2015.1060973
ER  - 

@article{
author = "Cabarkapa, Andrea and Borozan, Sunčica and Živković, Lada and Milanovic-Cabarkapa, Mirjana and Stojanović, Srđan and Bajic, Vladan and Spremo-Potparević, Biljana",
year = "2015",
abstract = "The aim of this study was to determine oxidative alterations leading to cellular dysfunctions in Pb-exposed subjects by evaluating damage to all major classes of biomolecules in the cell, lipid peroxidation, protein and DNA damage and determine relationships between parameters of Pb toxicity and specific biomarkers of oxidative damage.Analysis was conducted of smelter workers with high blood Pb and urine aminolevulinic acid levels and slightly elevated values of coproporphyrin and erythrocyte protoporphyrin IX. Significant decreases of thiol groups and increases in carbonyl groups as protein degradation end products, and of nitrite were detected. Elevated rates of lipid peroxidation and rises in the activities of the antioxidant enzymes Cu-Zn superoxide dismutase and catalase were also observed. Both enzymes showed positive correlations with the blood lead levels and urine coproporphyrin, while thiol groups correlated negatively with the same indices. The genotoxic potential of lead was manifested through an increased number of DNA-damaged cells. Increased activities of serum lactate dehydrogenase isoenzymes indicated cellular damage in the lungs, kidneys, and liver. These lead-induced impairments should be taken into consideration in the assessment of Pb-related health hazards.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Toxicological and Environmental Chemistry",
title = "Implications of oxidative stress in occupational exposure to lead on a cellular level",
volume = "97",
number = "6",
pages = "799-813",
doi = "10.1080/02772248.2015.1060973"
}

Cabarkapa, A., Borozan, S., Živković, L., Milanovic-Cabarkapa, M., Stojanović, S., Bajic, V.,& Spremo-Potparević, B.. (2015). Implications of oxidative stress in occupational exposure to lead on a cellular level. in Toxicological and Environmental Chemistry
Taylor & Francis Ltd, Abingdon., 97(6), 799-813.
https://doi.org/10.1080/02772248.2015.1060973

Cabarkapa A, Borozan S, Živković L, Milanovic-Cabarkapa M, Stojanović S, Bajic V, Spremo-Potparević B. Implications of oxidative stress in occupational exposure to lead on a cellular level. in Toxicological and Environmental Chemistry. 2015;97(6):799-813.
doi:10.1080/02772248.2015.1060973 .

Cabarkapa, Andrea, Borozan, Sunčica, Živković, Lada, Milanovic-Cabarkapa, Mirjana, Stojanović, Srđan, Bajic, Vladan, Spremo-Potparević, Biljana, "Implications of oxidative stress in occupational exposure to lead on a cellular level" in Toxicological and Environmental Chemistry, 97, no. 6 (2015):799-813,
https://doi.org/10.1080/02772248.2015.1060973 . .

TY  - JOUR
AU  - Cabarkapa, A
AU  - Borozan, Sunčica
AU  - Zivkovic, L
AU  - Stojanović, Srđan
AU  - Milanovic-Cabarkapa, M
AU  - Bajic, V
AU  - Spremo-Potparević, Biljana
PY  - 2015
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1800
AB  - Lead induced oxidative cellular damage and long-term persistence of associated adverse effects increases risk of late-onset diseases. CaNa(2)EDTA chelation is known to remove contaminating metals and to reduce free radical production. The objective was to investigate the impact of chelation therapy on modulation of lead induced cellular damage, restoration of altered enzyme activities and lipid homeostasis in peripheral blood of workers exposed to lead, by comparing the selected biomarkers obtained prior and after five-day CaNa(2)EDTA chelation intervention. The group of smelting factory workers diagnosed with lead intoxication and current lead exposure 5.8 +/- 1.2 years were administered five-day CaNa(2)EDTA chelation. Elevated baseline activity of antioxidant enzymes Cu, Zn-SOD and CAT as well as depleted thiols and increased protein degradation products-carbonyl groups and nitrites, pointing to Pb induced oxidative damage, were restored toward normal values following the treatment. Lead showed inhibitor potency on both RBC AChE and BChE in exposed workers, and chelation re-established the activity of BChE, while RBC AChE remained unaffected. Also, genotoxic effect of lead detected in peripheral blood lymphocytes was significantly decreased after therapy, exhibiting 18.9% DNA damage reduction. Administration of chelation reversed the depressed activity of serum PON 1 and significantly decreased lipid peroxidation detected by the post-chelation reduction of MDA levels. Lactate dehydrogenase LDF1-5 isoenzymes levels showed evident but no significant trend of restoring toward normal control values following chelation. CaNa(2)EDTA chelation ameliorates the alterations linked with Pb mediated oxidative stress, indicating possible benefits in reducing health risks associated with increased oxidative damage in lead exposed populations.
PB  - Elsevier Ireland Ltd, Clare
T2  - Chemico-Biological Interactions
T1  - CaNa(2)EDTA chelation attenuates cell damage in workers exposed to lead-a pilot study
VL  - 242
SP  - 171
EP  - 178
DO  - 10.1016/j.cbi.2015.10.002
ER  - 

@article{
author = "Cabarkapa, A and Borozan, Sunčica and Zivkovic, L and Stojanović, Srđan and Milanovic-Cabarkapa, M and Bajic, V and Spremo-Potparević, Biljana",
year = "2015",
abstract = "Lead induced oxidative cellular damage and long-term persistence of associated adverse effects increases risk of late-onset diseases. CaNa(2)EDTA chelation is known to remove contaminating metals and to reduce free radical production. The objective was to investigate the impact of chelation therapy on modulation of lead induced cellular damage, restoration of altered enzyme activities and lipid homeostasis in peripheral blood of workers exposed to lead, by comparing the selected biomarkers obtained prior and after five-day CaNa(2)EDTA chelation intervention. The group of smelting factory workers diagnosed with lead intoxication and current lead exposure 5.8 +/- 1.2 years were administered five-day CaNa(2)EDTA chelation. Elevated baseline activity of antioxidant enzymes Cu, Zn-SOD and CAT as well as depleted thiols and increased protein degradation products-carbonyl groups and nitrites, pointing to Pb induced oxidative damage, were restored toward normal values following the treatment. Lead showed inhibitor potency on both RBC AChE and BChE in exposed workers, and chelation re-established the activity of BChE, while RBC AChE remained unaffected. Also, genotoxic effect of lead detected in peripheral blood lymphocytes was significantly decreased after therapy, exhibiting 18.9% DNA damage reduction. Administration of chelation reversed the depressed activity of serum PON 1 and significantly decreased lipid peroxidation detected by the post-chelation reduction of MDA levels. Lactate dehydrogenase LDF1-5 isoenzymes levels showed evident but no significant trend of restoring toward normal control values following chelation. CaNa(2)EDTA chelation ameliorates the alterations linked with Pb mediated oxidative stress, indicating possible benefits in reducing health risks associated with increased oxidative damage in lead exposed populations.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Chemico-Biological Interactions",
title = "CaNa(2)EDTA chelation attenuates cell damage in workers exposed to lead-a pilot study",
volume = "242",
pages = "171-178",
doi = "10.1016/j.cbi.2015.10.002"
}

Cabarkapa, A., Borozan, S., Zivkovic, L., Stojanović, S., Milanovic-Cabarkapa, M., Bajic, V.,& Spremo-Potparević, B.. (2015). CaNa(2)EDTA chelation attenuates cell damage in workers exposed to lead-a pilot study. in Chemico-Biological Interactions
Elsevier Ireland Ltd, Clare., 242, 171-178.
https://doi.org/10.1016/j.cbi.2015.10.002

Cabarkapa A, Borozan S, Zivkovic L, Stojanović S, Milanovic-Cabarkapa M, Bajic V, Spremo-Potparević B. CaNa(2)EDTA chelation attenuates cell damage in workers exposed to lead-a pilot study. in Chemico-Biological Interactions. 2015;242:171-178.
doi:10.1016/j.cbi.2015.10.002 .

Cabarkapa, A, Borozan, Sunčica, Zivkovic, L, Stojanović, Srđan, Milanovic-Cabarkapa, M, Bajic, V, Spremo-Potparević, Biljana, "CaNa(2)EDTA chelation attenuates cell damage in workers exposed to lead-a pilot study" in Chemico-Biological Interactions, 242 (2015):171-178,
https://doi.org/10.1016/j.cbi.2015.10.002 . .

TY  - JOUR
AU  - Čabarkapa, Andrea
AU  - Borozan, Sunčica
AU  - Živković, Lada
AU  - Milanović-Čabarkapa, Mirjana
AU  - Stojanović, Srđan
AU  - Bajić, Vladan
AU  - Spremo-Potparević, Biljana
PY  - 2015
UR  - http://farfar.pharmacy.bg.ac.rs/handle/123456789/2440
UR  - http://farfar.pharmacy.bg.ac.rs/handle/123456789/3428
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3121
AB  - The aim of this study was to determine oxidative alterations leading to cellular dysfunctions in Pb-exposed subjects by evaluating damage to all major classes of biomolecules in the cell, lipid peroxidation, protein and DNA damage and determine relationships between parameters of Pb toxicity and specific biomarkers of oxidative damage.Analysis was conducted of smelter workers with high blood Pb and urine aminolevulinic acid levels and slightly elevated values of coproporphyrin and erythrocyte protoporphyrin IX. Significant decreases of thiol groups and increases in carbonyl groups as protein degradation end products, and of nitrite were detected. Elevated rates of lipid peroxidation and rises in the activities of the antioxidant enzymes Cu-Zn superoxide dismutase and catalase were also observed. Both enzymes showed positive correlations with the blood lead levels and urine coproporphyrin, while thiol groups correlated negatively with the same indices. The genotoxic potential of lead was manifested through an increased number of DNA-damaged cells. Increased activities of serum lactate dehydrogenase isoenzymes indicated cellular damage in the lungs, kidneys, and liver. These lead-induced impairments should be taken into consideration in the assessment of Pb-related health hazards.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Toxicology
T1  - Implications of oxidative stress in occupational exposure to lead on a cellular level
VL  - 97
IS  - 6
SP  - 799
EP  - 813
DO  - 10.1080/02772248.2015.1060973
ER  - 

@article{
author = "Čabarkapa, Andrea and Borozan, Sunčica and Živković, Lada and Milanović-Čabarkapa, Mirjana and Stojanović, Srđan and Bajić, Vladan and Spremo-Potparević, Biljana",
year = "2015",
abstract = "The aim of this study was to determine oxidative alterations leading to cellular dysfunctions in Pb-exposed subjects by evaluating damage to all major classes of biomolecules in the cell, lipid peroxidation, protein and DNA damage and determine relationships between parameters of Pb toxicity and specific biomarkers of oxidative damage.Analysis was conducted of smelter workers with high blood Pb and urine aminolevulinic acid levels and slightly elevated values of coproporphyrin and erythrocyte protoporphyrin IX. Significant decreases of thiol groups and increases in carbonyl groups as protein degradation end products, and of nitrite were detected. Elevated rates of lipid peroxidation and rises in the activities of the antioxidant enzymes Cu-Zn superoxide dismutase and catalase were also observed. Both enzymes showed positive correlations with the blood lead levels and urine coproporphyrin, while thiol groups correlated negatively with the same indices. The genotoxic potential of lead was manifested through an increased number of DNA-damaged cells. Increased activities of serum lactate dehydrogenase isoenzymes indicated cellular damage in the lungs, kidneys, and liver. These lead-induced impairments should be taken into consideration in the assessment of Pb-related health hazards.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Toxicology",
title = "Implications of oxidative stress in occupational exposure to lead on a cellular level",
volume = "97",
number = "6",
pages = "799-813",
doi = "10.1080/02772248.2015.1060973"
}

Čabarkapa, A., Borozan, S., Živković, L., Milanović-Čabarkapa, M., Stojanović, S., Bajić, V.,& Spremo-Potparević, B.. (2015). Implications of oxidative stress in occupational exposure to lead on a cellular level. in Toxicology
Taylor & Francis Ltd, Abingdon., 97(6), 799-813.
https://doi.org/10.1080/02772248.2015.1060973

Čabarkapa A, Borozan S, Živković L, Milanović-Čabarkapa M, Stojanović S, Bajić V, Spremo-Potparević B. Implications of oxidative stress in occupational exposure to lead on a cellular level. in Toxicology. 2015;97(6):799-813.
doi:10.1080/02772248.2015.1060973 .

Čabarkapa, Andrea, Borozan, Sunčica, Živković, Lada, Milanović-Čabarkapa, Mirjana, Stojanović, Srđan, Bajić, Vladan, Spremo-Potparević, Biljana, "Implications of oxidative stress in occupational exposure to lead on a cellular level" in Toxicology, 97, no. 6 (2015):799-813,
https://doi.org/10.1080/02772248.2015.1060973 . .

TY  - JOUR
AU  - Milicevic, Zorka
AU  - Bajic, Vladan
AU  - Živković, Lada
AU  - Kasapovic, Jelena
AU  - Anđelković, Uroš
AU  - Spremo-Potparević, Biljana
PY  - 2014
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1535
AB  - In breast carcinoma, disruption of the p53 pathway is one of the most common genetic alterations. The observation that the p53 can express multiple protein isoforms adds a novel level of complexity to the outcome of p53 mutations. p53 expression was analysed by Western immunoblotting and immunohistochemistry using monoclonal antibodies DO-7, Pab240, and polyclonal antiserum CM-1. The more frequently p53-positive nuclear staining has been found in the invasive breast tumors. One of the most intriguing findings is that mutant p53 appears as discrete dot-shaped regions within the nucleus of breast cancer cells. In many malignant cells, the nucleolar sequestration of p53 is evident. These observations support the view that the nucleolus is involved directly in the mediation of p53 function or indirectly by the control of the localization of p53 interplayers. p53 expressed in the nuclear fraction of breast cancer cells revealed a wide spectrum of isoforms. p53 isoforms Lambda Np53 (47 kDa) and Lambda 133p53 beta (35 kDa), known as dominant-negative repressors of p53 function, were detected as the most predominant variants in nuclei of invasive breast carcinoma cells. The isoforms expressed also varied between individual tumors, indicating potential roles of these p53 variants in human breast cancer.
PB  - Hindawi Publishing Corporation, New York
T2  - Scientific World Journal
T1  - Identification of p53 and Its Isoforms in Human Breast Carcinoma Cells
DO  - 10.1155/2014/618698
ER  - 

@article{
author = "Milicevic, Zorka and Bajic, Vladan and Živković, Lada and Kasapovic, Jelena and Anđelković, Uroš and Spremo-Potparević, Biljana",
year = "2014",
abstract = "In breast carcinoma, disruption of the p53 pathway is one of the most common genetic alterations. The observation that the p53 can express multiple protein isoforms adds a novel level of complexity to the outcome of p53 mutations. p53 expression was analysed by Western immunoblotting and immunohistochemistry using monoclonal antibodies DO-7, Pab240, and polyclonal antiserum CM-1. The more frequently p53-positive nuclear staining has been found in the invasive breast tumors. One of the most intriguing findings is that mutant p53 appears as discrete dot-shaped regions within the nucleus of breast cancer cells. In many malignant cells, the nucleolar sequestration of p53 is evident. These observations support the view that the nucleolus is involved directly in the mediation of p53 function or indirectly by the control of the localization of p53 interplayers. p53 expressed in the nuclear fraction of breast cancer cells revealed a wide spectrum of isoforms. p53 isoforms Lambda Np53 (47 kDa) and Lambda 133p53 beta (35 kDa), known as dominant-negative repressors of p53 function, were detected as the most predominant variants in nuclei of invasive breast carcinoma cells. The isoforms expressed also varied between individual tumors, indicating potential roles of these p53 variants in human breast cancer.",
publisher = "Hindawi Publishing Corporation, New York",
journal = "Scientific World Journal",
title = "Identification of p53 and Its Isoforms in Human Breast Carcinoma Cells",
doi = "10.1155/2014/618698"
}

Milicevic, Z., Bajic, V., Živković, L., Kasapovic, J., Anđelković, U.,& Spremo-Potparević, B.. (2014). Identification of p53 and Its Isoforms in Human Breast Carcinoma Cells. in Scientific World Journal
Hindawi Publishing Corporation, New York..
https://doi.org/10.1155/2014/618698

Milicevic Z, Bajic V, Živković L, Kasapovic J, Anđelković U, Spremo-Potparević B. Identification of p53 and Its Isoforms in Human Breast Carcinoma Cells. in Scientific World Journal. 2014;.
doi:10.1155/2014/618698 .

Milicevic, Zorka, Bajic, Vladan, Živković, Lada, Kasapovic, Jelena, Anđelković, Uroš, Spremo-Potparević, Biljana, "Identification of p53 and Its Isoforms in Human Breast Carcinoma Cells" in Scientific World Journal (2014),
https://doi.org/10.1155/2014/618698 . .

TY  - JOUR
AU  - Dimitrijević, Blagoje P.
AU  - Borozan, Sunčica
AU  - Jović, Slavoljub
AU  - Bacić, Dragan
AU  - Katić-Radivojević, Sofija
AU  - Stojanović, Srđan
AU  - Savić, Mila
PY  - 2013
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1157
AB  - The aim of this report was to study the biochemical parameters in sheep blood under conditions of various intensities of parasitic infection with Strongyloides papillosus, as well as after therapy with albendazole (ABZ). Investigations were performed on sheep of the Würtemberg race (n = 30) in which were detected mild, moderate and high intensities of parasitic infection with S. papillosus. The control group (n = 10) was composed of sheep negative to parasitic infections. The degree and type of changes were monitored by determining the concentrations of glucose, total proteins, albumin, A/G ratio, AST, urea, total bilirubin, calcium, phosphorus, total LDH activity and isoenzymatic LDH1-5 distributions. On the basis of the obtained results, we determined, through isoenzymatic LDH distribution, that during parasitic infection with S. papillosus, there is ongoing damage to the liver, heart muscle and lung, while after therapy with ABZ, the liver suffers the most damage. The concentration of glucose, total proteins and albumin fell linearly with the rise in the intensity of parasitic infection (p LT 0.05), while after therapy with ABZ, the fall of these parameters was at a statistically significantly higher level (p LT 0.01). The activity of AST, concentration of urea and total bilirubin also rose linearly with the intensity of parasitic infection (p LT 0.05). After therapy with ABZ, the activity of AST and the concentration of total bilirubin were at a statistically significantly higher level (p LT 0.001), while the concentration of urea retained the same levels as in the case of parasitic infection. Values of calcium concentrations (p>0.05) and phosphorus (p LT 0.05) also fall linearly with the rise of the intesity of the parasitic infection. The trend in the concentration fall of these macroelements, continues also after treatment with albendazole (p LT 0.001). Having in mind our previous studies in the field of oxidative stress and phenomena lying behind these changes, we strongly recommend that in antiparasitic treatment protocols, beside antihelminthics, compounds with antioxidative properties should also be used.
AB  - Cilj ovog rada je bio da se ispitaju biohemijski parametri u krvi ovaca u uslovima različitog intenziteta parazitske infekcije sa Strongyloides papillosus, kao i nakon terapije sa albendazolom (ABZ). Istraživanje je izvedeno na ovcama Wurtemberg rase (n = 30) kod kojih je utvrđen blagi, srednji i visoki intenzitet parazitske infekcije sa S. papillosus. Kontrolnu grupu (n = 10) činile su ovce negativne na prisustvo parazitske infekcije. Stepen i vrsta promene praćeni su određivanjem koncentracije glukoze, ukupnih proteina, albumina, A/G ratio, AST, urea, ukupnog bilirubina, kalcijuma, fosfora, ukupne aktivnosti LDH i izoenzimske distribucije LDH1-5. Na osnovu dobijenih rezultata utvrdili smo, preko izoenzimske distribucije LDH, da u toku parazitske infekcije sa S. papillosus dolazi do oštećenja jetre, srčanog mišića i pluća, dok nakon terapije sa ABZ jetra je organ koji je najviše oštećen. Koncentracija glukoze, ukupnih proteina i albumina linearno je opadala sa rastom intenziteta parazitske infekcije (p LT 0.05), a nakon terapije sa ABZ pad koncentracije ovih parametara bio je na statistički značajnom većem nivou (p LT 0.01). Aktivnost AST, koncentracija uree i ukupnog bilirubina takođe su linearno rasle sa intenzitetom parazitske infekcije (p LT 0.05). Nakon terapije sa ABZ aktivnost AST i koncentracija ukupnog bilirubina bile su na statistički značajno većem nivou (p LT 0.001), dok je koncentracija uree zadržala iste nivoe kao u slučaju parazitske infekcije. Vrednosti koncentracija kalcijuma (p > 0.05) i fosfora (p LT 0.05) takođe linearno opadaju sa rastom intenziteta parazitske infekcije. Trend pada koncentracije ovih makroelemenata, nastavlja se i nakon terapije sa albendazolom (p LT 0.001). Imajući u vidu naša prethodna istraživanja na polju oksidativnog stresa, fenomena koji se nalazi u osnovi ovih promena, strogo preporučujemo da se u antiparazitske protokole, pored antihelmintika koriste i preparati sa antioksidativnim osobinama.
PB  - Veterinary Faculty, Univ Beogradu, Belgrade
T2  - Acta veterinaria
T1  - The effect of the intensity of parasitic infection with Strongyloides papillosus and albendazole therapy on biochemical parameters in sheep blood
T1  - Uticaj intenziteta parazitske infekcije sa Strongyloides papillosus i terapije albendazolom na biohemijske parametre u krvi ovaca
VL  - 63
IS  - 5-6
SP  - 581
EP  - 600
DO  - 10.2298/AVB1306581D
ER  - 

@article{
author = "Dimitrijević, Blagoje P. and Borozan, Sunčica and Jović, Slavoljub and Bacić, Dragan and Katić-Radivojević, Sofija and Stojanović, Srđan and Savić, Mila",
year = "2013",
abstract = "The aim of this report was to study the biochemical parameters in sheep blood under conditions of various intensities of parasitic infection with Strongyloides papillosus, as well as after therapy with albendazole (ABZ). Investigations were performed on sheep of the Würtemberg race (n = 30) in which were detected mild, moderate and high intensities of parasitic infection with S. papillosus. The control group (n = 10) was composed of sheep negative to parasitic infections. The degree and type of changes were monitored by determining the concentrations of glucose, total proteins, albumin, A/G ratio, AST, urea, total bilirubin, calcium, phosphorus, total LDH activity and isoenzymatic LDH1-5 distributions. On the basis of the obtained results, we determined, through isoenzymatic LDH distribution, that during parasitic infection with S. papillosus, there is ongoing damage to the liver, heart muscle and lung, while after therapy with ABZ, the liver suffers the most damage. The concentration of glucose, total proteins and albumin fell linearly with the rise in the intensity of parasitic infection (p LT 0.05), while after therapy with ABZ, the fall of these parameters was at a statistically significantly higher level (p LT 0.01). The activity of AST, concentration of urea and total bilirubin also rose linearly with the intensity of parasitic infection (p LT 0.05). After therapy with ABZ, the activity of AST and the concentration of total bilirubin were at a statistically significantly higher level (p LT 0.001), while the concentration of urea retained the same levels as in the case of parasitic infection. Values of calcium concentrations (p>0.05) and phosphorus (p LT 0.05) also fall linearly with the rise of the intesity of the parasitic infection. The trend in the concentration fall of these macroelements, continues also after treatment with albendazole (p LT 0.001). Having in mind our previous studies in the field of oxidative stress and phenomena lying behind these changes, we strongly recommend that in antiparasitic treatment protocols, beside antihelminthics, compounds with antioxidative properties should also be used., Cilj ovog rada je bio da se ispitaju biohemijski parametri u krvi ovaca u uslovima različitog intenziteta parazitske infekcije sa Strongyloides papillosus, kao i nakon terapije sa albendazolom (ABZ). Istraživanje je izvedeno na ovcama Wurtemberg rase (n = 30) kod kojih je utvrđen blagi, srednji i visoki intenzitet parazitske infekcije sa S. papillosus. Kontrolnu grupu (n = 10) činile su ovce negativne na prisustvo parazitske infekcije. Stepen i vrsta promene praćeni su određivanjem koncentracije glukoze, ukupnih proteina, albumina, A/G ratio, AST, urea, ukupnog bilirubina, kalcijuma, fosfora, ukupne aktivnosti LDH i izoenzimske distribucije LDH1-5. Na osnovu dobijenih rezultata utvrdili smo, preko izoenzimske distribucije LDH, da u toku parazitske infekcije sa S. papillosus dolazi do oštećenja jetre, srčanog mišića i pluća, dok nakon terapije sa ABZ jetra je organ koji je najviše oštećen. Koncentracija glukoze, ukupnih proteina i albumina linearno je opadala sa rastom intenziteta parazitske infekcije (p LT 0.05), a nakon terapije sa ABZ pad koncentracije ovih parametara bio je na statistički značajnom većem nivou (p LT 0.01). Aktivnost AST, koncentracija uree i ukupnog bilirubina takođe su linearno rasle sa intenzitetom parazitske infekcije (p LT 0.05). Nakon terapije sa ABZ aktivnost AST i koncentracija ukupnog bilirubina bile su na statistički značajno većem nivou (p LT 0.001), dok je koncentracija uree zadržala iste nivoe kao u slučaju parazitske infekcije. Vrednosti koncentracija kalcijuma (p > 0.05) i fosfora (p LT 0.05) takođe linearno opadaju sa rastom intenziteta parazitske infekcije. Trend pada koncentracije ovih makroelemenata, nastavlja se i nakon terapije sa albendazolom (p LT 0.001). Imajući u vidu naša prethodna istraživanja na polju oksidativnog stresa, fenomena koji se nalazi u osnovi ovih promena, strogo preporučujemo da se u antiparazitske protokole, pored antihelmintika koriste i preparati sa antioksidativnim osobinama.",
publisher = "Veterinary Faculty, Univ Beogradu, Belgrade",
journal = "Acta veterinaria",
title = "The effect of the intensity of parasitic infection with Strongyloides papillosus and albendazole therapy on biochemical parameters in sheep blood, Uticaj intenziteta parazitske infekcije sa Strongyloides papillosus i terapije albendazolom na biohemijske parametre u krvi ovaca",
volume = "63",
number = "5-6",
pages = "581-600",
doi = "10.2298/AVB1306581D"
}

Dimitrijević, B. P., Borozan, S., Jović, S., Bacić, D., Katić-Radivojević, S., Stojanović, S.,& Savić, M.. (2013). The effect of the intensity of parasitic infection with Strongyloides papillosus and albendazole therapy on biochemical parameters in sheep blood. in Acta veterinaria
Veterinary Faculty, Univ Beogradu, Belgrade., 63(5-6), 581-600.
https://doi.org/10.2298/AVB1306581D

Dimitrijević BP, Borozan S, Jović S, Bacić D, Katić-Radivojević S, Stojanović S, Savić M. The effect of the intensity of parasitic infection with Strongyloides papillosus and albendazole therapy on biochemical parameters in sheep blood. in Acta veterinaria. 2013;63(5-6):581-600.
doi:10.2298/AVB1306581D .

Dimitrijević, Blagoje P., Borozan, Sunčica, Jović, Slavoljub, Bacić, Dragan, Katić-Radivojević, Sofija, Stojanović, Srđan, Savić, Mila, "The effect of the intensity of parasitic infection with Strongyloides papillosus and albendazole therapy on biochemical parameters in sheep blood" in Acta veterinaria, 63, no. 5-6 (2013):581-600,
https://doi.org/10.2298/AVB1306581D . .

TY  - JOUR
AU  - Borozan, Sunčica
AU  - Stojanović, Srđan
PY  - 2013
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1235
AB  - In this work, we have analyzed the influence of halogen bonding to the stability of 44 complexes of proteins and non-natural amino acids. Fluorine- and chlorine-containing non-natural amino acids are more prevalent in the dataset, and an even larger number of contacts made by iodine-containing ligands are found. Only few halogen bonds with the hydroxyl oxygens and carboxylate side chains are found in the dataset. Halogen bonds with the nitrogen-containing side chains have higher occurrence than other acceptors. Backbone carbonyl oxygens and nitrogens are to a substantial extent involved in our dataset We have observed a small percentage of interactions involving water as hydrogen bond donors. Additionally, most of the interacting residues comprising the interfaces also show a great degree of conservation. There is a clear interaction hot spot at distances of 3.5-3.7 angstrom and Theta(1) angles of 100-120 degrees. There is also a cluster of contacts featuring short distances (2.6-2.9 angstrom) but only nearly optimal Theta(1) angles (140-160 degrees). 51.3% of stabilizing residues are involved in building halogen bonds with the non-natural amino acids. We discovered three types of structural motifs significantly over-represented: beta-turn-ir, beta-turn-il and niche-4r. The halogen-bonding statistics of the dataset do not show any preference for alpha-helices (36%), beta-sheets (36%), or turns/coils (28%) structures. Most of the amino acid residues that were involved in halogen bonds prefer to be in the solvent excluded environment (buried). Furthermore, we have shown that in amino acid-protein complexes halogen atoms can sometimes be involved in hydrogen bonding interactions with hydrogen bonding-donors. The results from this study might be used for the rational design of halogenated ligands as inhibitors and drugs, and in biomolecular engineering.
PB  - Elsevier Sci Ltd, Oxford
T2  - Computational Biology and Chemistry
T1  - Halogen bonding in complexes of proteins and non-natural amino acids
VL  - 47
SP  - 231
EP  - 239
DO  - 10.1016/j.compbiolchem.2013.10.002
ER  - 

@article{
author = "Borozan, Sunčica and Stojanović, Srđan",
year = "2013",
abstract = "In this work, we have analyzed the influence of halogen bonding to the stability of 44 complexes of proteins and non-natural amino acids. Fluorine- and chlorine-containing non-natural amino acids are more prevalent in the dataset, and an even larger number of contacts made by iodine-containing ligands are found. Only few halogen bonds with the hydroxyl oxygens and carboxylate side chains are found in the dataset. Halogen bonds with the nitrogen-containing side chains have higher occurrence than other acceptors. Backbone carbonyl oxygens and nitrogens are to a substantial extent involved in our dataset We have observed a small percentage of interactions involving water as hydrogen bond donors. Additionally, most of the interacting residues comprising the interfaces also show a great degree of conservation. There is a clear interaction hot spot at distances of 3.5-3.7 angstrom and Theta(1) angles of 100-120 degrees. There is also a cluster of contacts featuring short distances (2.6-2.9 angstrom) but only nearly optimal Theta(1) angles (140-160 degrees). 51.3% of stabilizing residues are involved in building halogen bonds with the non-natural amino acids. We discovered three types of structural motifs significantly over-represented: beta-turn-ir, beta-turn-il and niche-4r. The halogen-bonding statistics of the dataset do not show any preference for alpha-helices (36%), beta-sheets (36%), or turns/coils (28%) structures. Most of the amino acid residues that were involved in halogen bonds prefer to be in the solvent excluded environment (buried). Furthermore, we have shown that in amino acid-protein complexes halogen atoms can sometimes be involved in hydrogen bonding interactions with hydrogen bonding-donors. The results from this study might be used for the rational design of halogenated ligands as inhibitors and drugs, and in biomolecular engineering.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Computational Biology and Chemistry",
title = "Halogen bonding in complexes of proteins and non-natural amino acids",
volume = "47",
pages = "231-239",
doi = "10.1016/j.compbiolchem.2013.10.002"
}

Borozan, S.,& Stojanović, S.. (2013). Halogen bonding in complexes of proteins and non-natural amino acids. in Computational Biology and Chemistry
Elsevier Sci Ltd, Oxford., 47, 231-239.
https://doi.org/10.1016/j.compbiolchem.2013.10.002

Borozan S, Stojanović S. Halogen bonding in complexes of proteins and non-natural amino acids. in Computational Biology and Chemistry. 2013;47:231-239.
doi:10.1016/j.compbiolchem.2013.10.002 .

Borozan, Sunčica, Stojanović, Srđan, "Halogen bonding in complexes of proteins and non-natural amino acids" in Computational Biology and Chemistry, 47 (2013):231-239,
https://doi.org/10.1016/j.compbiolchem.2013.10.002 . .

TY  - JOUR
AU  - Borozan, Sunčica
AU  - Dimitrijević, Blagoje P.
AU  - Stojanović, Srđan
PY  - 2013
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1247
AB  - In this work, we have analyzed the influence of cation-pi interactions to the stability of 59 high resolution protein-RNA complex crystal structures. The total number of Lys and Arg are similar in the dataset as well as the number of their interactions. On the other hand, the aromatic chains of purines are exhibiting more cation-pi interactions than pyrimidines. 35% of the total interactions in the dataset are involved in the formation of multiple cation-pi interactions. The multiple cation-pi interactions have been conserved more than the single interactions. The analysis of the geometry of the cation-pi interactions has revealed that the average distance (d) value falls into distinct ranges corresponding to the multiple (4.28 angstrom) and single (5.50 angstrom) cation-pi interactions. The G-Arg pair has the strongest interaction energy of -3.68 kcal mol(-1) among all the possible pairs of amino acids and bases. Further, we found that the cation-pi interactions due to five-membered rings of A and G are stronger than that with the atoms in six-membered rings. 8.7% stabilizing residues are involved in building cation-pi interactions with the nucleic bases. There are three types of structural motifs significantly over-represented in protein-RNA interfaces: beta-turn-ir, niche-4r and st-staple. Tetraloops and kink-turns are the most abundant RNA motifs in protein-RNA interfaces. Amino acids deployed in the protein-RNA interfaces are deposited in helices, sheets and coils. Arg and Lys, involved in cation-pi interactions, prefer to be in the solvent exposed surface. The results from this study might be used for structure-based prediction and as scaffolds for future protein-RNA complex design.
PB  - Elsevier Sci Ltd, Oxford
T2  - Computational Biology and Chemistry
T1  - Cation-pi interactions in high resolution protein-RNA complex crystal structures
VL  - 47
SP  - 105
EP  - 112
DO  - 10.1016/j.compbiolchem.2013.08.005
ER  - 

@article{
author = "Borozan, Sunčica and Dimitrijević, Blagoje P. and Stojanović, Srđan",
year = "2013",
abstract = "In this work, we have analyzed the influence of cation-pi interactions to the stability of 59 high resolution protein-RNA complex crystal structures. The total number of Lys and Arg are similar in the dataset as well as the number of their interactions. On the other hand, the aromatic chains of purines are exhibiting more cation-pi interactions than pyrimidines. 35% of the total interactions in the dataset are involved in the formation of multiple cation-pi interactions. The multiple cation-pi interactions have been conserved more than the single interactions. The analysis of the geometry of the cation-pi interactions has revealed that the average distance (d) value falls into distinct ranges corresponding to the multiple (4.28 angstrom) and single (5.50 angstrom) cation-pi interactions. The G-Arg pair has the strongest interaction energy of -3.68 kcal mol(-1) among all the possible pairs of amino acids and bases. Further, we found that the cation-pi interactions due to five-membered rings of A and G are stronger than that with the atoms in six-membered rings. 8.7% stabilizing residues are involved in building cation-pi interactions with the nucleic bases. There are three types of structural motifs significantly over-represented in protein-RNA interfaces: beta-turn-ir, niche-4r and st-staple. Tetraloops and kink-turns are the most abundant RNA motifs in protein-RNA interfaces. Amino acids deployed in the protein-RNA interfaces are deposited in helices, sheets and coils. Arg and Lys, involved in cation-pi interactions, prefer to be in the solvent exposed surface. The results from this study might be used for structure-based prediction and as scaffolds for future protein-RNA complex design.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Computational Biology and Chemistry",
title = "Cation-pi interactions in high resolution protein-RNA complex crystal structures",
volume = "47",
pages = "105-112",
doi = "10.1016/j.compbiolchem.2013.08.005"
}

Borozan, S., Dimitrijević, B. P.,& Stojanović, S.. (2013). Cation-pi interactions in high resolution protein-RNA complex crystal structures. in Computational Biology and Chemistry
Elsevier Sci Ltd, Oxford., 47, 105-112.
https://doi.org/10.1016/j.compbiolchem.2013.08.005

Borozan S, Dimitrijević BP, Stojanović S. Cation-pi interactions in high resolution protein-RNA complex crystal structures. in Computational Biology and Chemistry. 2013;47:105-112.
doi:10.1016/j.compbiolchem.2013.08.005 .

Borozan, Sunčica, Dimitrijević, Blagoje P., Stojanović, Srđan, "Cation-pi interactions in high resolution protein-RNA complex crystal structures" in Computational Biology and Chemistry, 47 (2013):105-112,
https://doi.org/10.1016/j.compbiolchem.2013.08.005 . .

TY  - JOUR
AU  - Dimitrijević, Blagoje P.
AU  - Borozan, Sunčica
AU  - Katic-Radivojevic, Sofija
AU  - Stojanović, Srđan
PY  - 2012
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/944
AB  - The objective of this study was to estimate and evaluate oxidative/nitrosative stress parameters in sheep infected with Strongyloides papillosus and after antihelminthic treatment with albendazole (ABZ). This parasite, especially during development stages can seriously damage parenchaematous organs during migration within the host. The presence of parasites leads to increased productions of reactive oxygen species (ROS) and reactive nitrogen species (RNS). It is also well known that certain drugs can be very harmful for the delicate oxidant-antioxidant equilibrium, provoking oxidative stress during their biotransformation. ABZ is a broad spectrum antihelminthic drug, frequently used in veterinary medicine for therapy of parasitic infections. The current research was performed on female Wurttemberg sheep (n = 48). The distribution of parasites in sheep was evaluated using the native smear coprological technique, by sedimentation and flotation methods, revealing the presence of S. papillosus. The degree of infection intensity per sheep was quantitatively established by the method of McMaster, the animals having been divided into three groups according to the intensity of infection; mild, moderate and high. The control group consisted of sheep negative to the parasites. After determining the type of parasite infection, the sheep were treated with ABZ, per orally, in single doses of 5 mg/kg per body weight. Sampling of feces for parasitological and blood for biochemical assaying was performed on the 0 and 21st day after treatment with ABZ. The oxidative stress parameters were measured for catalase activity (CAT), the red cell membrane damage by level of malondialdehyde (MDA), while carbonyl and thiol plasma protein group concentrations were used as indicators of the degree of protein oxidative modification. The activity of Cu,Zn-superoxide dismutase (SOD) and relative distribution of lactate dehydrogenase (LDH1-LDH5) activity were determined electrophoretically. The distribution of LDH isoenzymes in sheep moderately and highly infected with S. papillosus revealed that the parasite induced damage to the myocardial (LDH2), lung (LDH3) and liver cells (LDH5) in infected animals, while ABZ treatment only damaged liver cells (LDH5). The MDA concentration revealed that lipid peroxidation increased both in the presence of parasites and the antihelminthic formulation tested (p  LT  0.001) when compared to the control sheep, while the increase of carbonyl concentration (p  LT  0.001), as well as the observed decrease of thiol concentration (p  LT  0.001) indicated significant oxidative damage of plasma proteins in experimental sheep, when compared to the control animals. Our results indicate that S. papillosus induces oxidative/nitrosative stress in sheep. The antihelminthic treatment with ABZ further promotes the disbalance of oxidative-antioxidative equilibrium in all tested sheep.
PB  - Elsevier
T2  - Veterinary Parasitology
T1  - Effects of infection intensity with Strongyloides papillosus and albendazole treatment on development of oxidative/nitrosative stress in sheep
VL  - 186
IS  - 3-4
SP  - 364
EP  - 375
DO  - 10.1016/j.vetpar.2011.11.017
ER  - 

@article{
author = "Dimitrijević, Blagoje P. and Borozan, Sunčica and Katic-Radivojevic, Sofija and Stojanović, Srđan",
year = "2012",
abstract = "The objective of this study was to estimate and evaluate oxidative/nitrosative stress parameters in sheep infected with Strongyloides papillosus and after antihelminthic treatment with albendazole (ABZ). This parasite, especially during development stages can seriously damage parenchaematous organs during migration within the host. The presence of parasites leads to increased productions of reactive oxygen species (ROS) and reactive nitrogen species (RNS). It is also well known that certain drugs can be very harmful for the delicate oxidant-antioxidant equilibrium, provoking oxidative stress during their biotransformation. ABZ is a broad spectrum antihelminthic drug, frequently used in veterinary medicine for therapy of parasitic infections. The current research was performed on female Wurttemberg sheep (n = 48). The distribution of parasites in sheep was evaluated using the native smear coprological technique, by sedimentation and flotation methods, revealing the presence of S. papillosus. The degree of infection intensity per sheep was quantitatively established by the method of McMaster, the animals having been divided into three groups according to the intensity of infection; mild, moderate and high. The control group consisted of sheep negative to the parasites. After determining the type of parasite infection, the sheep were treated with ABZ, per orally, in single doses of 5 mg/kg per body weight. Sampling of feces for parasitological and blood for biochemical assaying was performed on the 0 and 21st day after treatment with ABZ. The oxidative stress parameters were measured for catalase activity (CAT), the red cell membrane damage by level of malondialdehyde (MDA), while carbonyl and thiol plasma protein group concentrations were used as indicators of the degree of protein oxidative modification. The activity of Cu,Zn-superoxide dismutase (SOD) and relative distribution of lactate dehydrogenase (LDH1-LDH5) activity were determined electrophoretically. The distribution of LDH isoenzymes in sheep moderately and highly infected with S. papillosus revealed that the parasite induced damage to the myocardial (LDH2), lung (LDH3) and liver cells (LDH5) in infected animals, while ABZ treatment only damaged liver cells (LDH5). The MDA concentration revealed that lipid peroxidation increased both in the presence of parasites and the antihelminthic formulation tested (p  LT  0.001) when compared to the control sheep, while the increase of carbonyl concentration (p  LT  0.001), as well as the observed decrease of thiol concentration (p  LT  0.001) indicated significant oxidative damage of plasma proteins in experimental sheep, when compared to the control animals. Our results indicate that S. papillosus induces oxidative/nitrosative stress in sheep. The antihelminthic treatment with ABZ further promotes the disbalance of oxidative-antioxidative equilibrium in all tested sheep.",
publisher = "Elsevier",
journal = "Veterinary Parasitology",
title = "Effects of infection intensity with Strongyloides papillosus and albendazole treatment on development of oxidative/nitrosative stress in sheep",
volume = "186",
number = "3-4",
pages = "364-375",
doi = "10.1016/j.vetpar.2011.11.017"
}

Dimitrijević, B. P., Borozan, S., Katic-Radivojevic, S.,& Stojanović, S.. (2012). Effects of infection intensity with Strongyloides papillosus and albendazole treatment on development of oxidative/nitrosative stress in sheep. in Veterinary Parasitology
Elsevier., 186(3-4), 364-375.
https://doi.org/10.1016/j.vetpar.2011.11.017

Dimitrijević BP, Borozan S, Katic-Radivojevic S, Stojanović S. Effects of infection intensity with Strongyloides papillosus and albendazole treatment on development of oxidative/nitrosative stress in sheep. in Veterinary Parasitology. 2012;186(3-4):364-375.
doi:10.1016/j.vetpar.2011.11.017 .

Dimitrijević, Blagoje P., Borozan, Sunčica, Katic-Radivojevic, Sofija, Stojanović, Srđan, "Effects of infection intensity with Strongyloides papillosus and albendazole treatment on development of oxidative/nitrosative stress in sheep" in Veterinary Parasitology, 186, no. 3-4 (2012):364-375,
https://doi.org/10.1016/j.vetpar.2011.11.017 . .

TY  - JOUR
AU  - Dimitrijević, Blagoje P.
AU  - Borozan, Sunčica
AU  - Stojanović, Srđan
PY  - 2012
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1119
AB  - In this study we have described the pi-pi and cation-pi interactions between the porphyrin ring and the protein part of porphyrin-containing proteins to better understand their stabilizing role. The number of pi-pi interactions was higher than that of cation-pi interactions in the same set of proteins studied. The pyrrole groups of one porphyrin can be involved in pi-pi interactions with pi systems of another porphyrin in the protein. We have found 5.1% cation-pi interactions between porphyrin Fe2+ metal cations and pi systems of surrounding amino acids as well as the pyrrole rings of other porphyrins. We observed that most of the pi-pi interactions have an energy in the range -0.5 to -2.0 kcal mol(-1), while the cation-pi interactions showed an energy in the range 22 to -4 kcal mol(-1). Further, an appreciable number of metal/cation-pi interaction pairs have an energy in the range -6 to -13 kcal mol(-1). The preferred parallel-stacked orientation is found to be more stable than a T-shaped structure for the full set of pi-pi interaction pairs. In the case of cation-pi interactions, it was found that 44% of the cation-pi interactions involved planar stacking, 37% of the interactions belonged to the oblique category, and the remaining 19% of the interactions were of the orthogonal type. The separation distance between the cation group and the aromatic ring decreases as the interplanar angle decreases. Furthermore, in the present study we have found that 10.4% of pi residues and 3.9% of cationic residues were found to have one or more stabilization centers. Amino acids deployed in the environment of porphyrin rings are deposited in helices and coils. The results from this study might be used for structure-based porphyrin protein prediction and as scaffolds for future porphyrin-containing protein design.
PB  - The Royal Society of Chemistry
T2  - RSC Advances
T1  - pi-pi and cation-pi interactions in protein-porphyrin complex crystal structures
VL  - 2
IS  - 33
SP  - 12963
EP  - 12972
DO  - 10.1039/c2ra21937a
ER  - 

@article{
author = "Dimitrijević, Blagoje P. and Borozan, Sunčica and Stojanović, Srđan",
year = "2012",
abstract = "In this study we have described the pi-pi and cation-pi interactions between the porphyrin ring and the protein part of porphyrin-containing proteins to better understand their stabilizing role. The number of pi-pi interactions was higher than that of cation-pi interactions in the same set of proteins studied. The pyrrole groups of one porphyrin can be involved in pi-pi interactions with pi systems of another porphyrin in the protein. We have found 5.1% cation-pi interactions between porphyrin Fe2+ metal cations and pi systems of surrounding amino acids as well as the pyrrole rings of other porphyrins. We observed that most of the pi-pi interactions have an energy in the range -0.5 to -2.0 kcal mol(-1), while the cation-pi interactions showed an energy in the range 22 to -4 kcal mol(-1). Further, an appreciable number of metal/cation-pi interaction pairs have an energy in the range -6 to -13 kcal mol(-1). The preferred parallel-stacked orientation is found to be more stable than a T-shaped structure for the full set of pi-pi interaction pairs. In the case of cation-pi interactions, it was found that 44% of the cation-pi interactions involved planar stacking, 37% of the interactions belonged to the oblique category, and the remaining 19% of the interactions were of the orthogonal type. The separation distance between the cation group and the aromatic ring decreases as the interplanar angle decreases. Furthermore, in the present study we have found that 10.4% of pi residues and 3.9% of cationic residues were found to have one or more stabilization centers. Amino acids deployed in the environment of porphyrin rings are deposited in helices and coils. The results from this study might be used for structure-based porphyrin protein prediction and as scaffolds for future porphyrin-containing protein design.",
publisher = "The Royal Society of Chemistry",
journal = "RSC Advances",
title = "pi-pi and cation-pi interactions in protein-porphyrin complex crystal structures",
volume = "2",
number = "33",
pages = "12963-12972",
doi = "10.1039/c2ra21937a"
}

Dimitrijević, B. P., Borozan, S.,& Stojanović, S.. (2012). pi-pi and cation-pi interactions in protein-porphyrin complex crystal structures. in RSC Advances
The Royal Society of Chemistry., 2(33), 12963-12972.
https://doi.org/10.1039/c2ra21937a

Dimitrijević BP, Borozan S, Stojanović S. pi-pi and cation-pi interactions in protein-porphyrin complex crystal structures. in RSC Advances. 2012;2(33):12963-12972.
doi:10.1039/c2ra21937a .

Dimitrijević, Blagoje P., Borozan, Sunčica, Stojanović, Srđan, "pi-pi and cation-pi interactions in protein-porphyrin complex crystal structures" in RSC Advances, 2, no. 33 (2012):12963-12972,
https://doi.org/10.1039/c2ra21937a . .