TY  - CONF
AU  - Lugonja, Nikoleta
AU  - Laugier, Olga B.
AU  - Spasić, Snežana
AU  - Gojgić-Cvijović, Gordana
AU  - Vrvić, Miroslav
PY  - 2012
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5279
AB  - Poster presented at: International Symposium of Probiotics & Prebiotics in Pediatrics, Istanbul, Turkey, Feb 24-26, 2012
T1  - Bifidogenic effect of a follow-on formula supplemented with prebiotics
UR  - https://hdl.handle.net/21.15107/rcub_cer_5279
ER  - 

@conference{
author = "Lugonja, Nikoleta and Laugier, Olga B. and Spasić, Snežana and Gojgić-Cvijović, Gordana and Vrvić, Miroslav",
year = "2012",
abstract = "Poster presented at: International Symposium of Probiotics & Prebiotics in Pediatrics, Istanbul, Turkey, Feb 24-26, 2012",
title = "Bifidogenic effect of a follow-on formula supplemented with prebiotics",
url = "https://hdl.handle.net/21.15107/rcub_cer_5279"
}

Lugonja, N., Laugier, O. B., Spasić, S., Gojgić-Cvijović, G.,& Vrvić, M.. (2012). Bifidogenic effect of a follow-on formula supplemented with prebiotics. .
https://hdl.handle.net/21.15107/rcub_cer_5279

Lugonja N, Laugier OB, Spasić S, Gojgić-Cvijović G, Vrvić M. Bifidogenic effect of a follow-on formula supplemented with prebiotics. 2012;.
https://hdl.handle.net/21.15107/rcub_cer_5279 .

Lugonja, Nikoleta, Laugier, Olga B., Spasić, Snežana, Gojgić-Cvijović, Gordana, Vrvić, Miroslav, "Bifidogenic effect of a follow-on formula supplemented with prebiotics" (2012),
https://hdl.handle.net/21.15107/rcub_cer_5279 .

TY  - CONF
AU  - Lugonja, Nikoleta
AU  - Laugier, Olga B.
AU  - Spasić, Snežana
AU  - Gojgić-Cvijović, Gordana
AU  - Vrvić, Miroslav
PY  - 2012
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5278
AB  - The bifidogenic effect is a stimulation of the growth and metabolism of bifidogenic bacteria in the gut. The aim of this study was to determine the bifidogenic effect of a follow-on formula supplemented with inulin and fructooligosaccharides (FOS), and compare it to that of human breast milk.
PB  - Istanbul, turkey : Eskisehir Osmangazi University Faculty of Medicine
C3  - Program and abstract book - International Symposium of Probiotics & Prebiotics in Pediatrics, Istanbul, Turkey, Feb 24-26, 2012
T1  - Bifidogenic effect of a follow-on formula supplemented with prebiotics
SP  - 82
UR  - https://hdl.handle.net/21.15107/rcub_cer_5278
ER  - 

@conference{
author = "Lugonja, Nikoleta and Laugier, Olga B. and Spasić, Snežana and Gojgić-Cvijović, Gordana and Vrvić, Miroslav",
year = "2012",
abstract = "The bifidogenic effect is a stimulation of the growth and metabolism of bifidogenic bacteria in the gut. The aim of this study was to determine the bifidogenic effect of a follow-on formula supplemented with inulin and fructooligosaccharides (FOS), and compare it to that of human breast milk.",
publisher = "Istanbul, turkey : Eskisehir Osmangazi University Faculty of Medicine",
journal = "Program and abstract book - International Symposium of Probiotics & Prebiotics in Pediatrics, Istanbul, Turkey, Feb 24-26, 2012",
title = "Bifidogenic effect of a follow-on formula supplemented with prebiotics",
pages = "82",
url = "https://hdl.handle.net/21.15107/rcub_cer_5278"
}

Lugonja, N., Laugier, O. B., Spasić, S., Gojgić-Cvijović, G.,& Vrvić, M.. (2012). Bifidogenic effect of a follow-on formula supplemented with prebiotics. in Program and abstract book - International Symposium of Probiotics & Prebiotics in Pediatrics, Istanbul, Turkey, Feb 24-26, 2012
Istanbul, turkey : Eskisehir Osmangazi University Faculty of Medicine., 82.
https://hdl.handle.net/21.15107/rcub_cer_5278

Lugonja N, Laugier OB, Spasić S, Gojgić-Cvijović G, Vrvić M. Bifidogenic effect of a follow-on formula supplemented with prebiotics. in Program and abstract book - International Symposium of Probiotics & Prebiotics in Pediatrics, Istanbul, Turkey, Feb 24-26, 2012. 2012;:82.
https://hdl.handle.net/21.15107/rcub_cer_5278 .

Lugonja, Nikoleta, Laugier, Olga B., Spasić, Snežana, Gojgić-Cvijović, Gordana, Vrvić, Miroslav, "Bifidogenic effect of a follow-on formula supplemented with prebiotics" in Program and abstract book - International Symposium of Probiotics & Prebiotics in Pediatrics, Istanbul, Turkey, Feb 24-26, 2012 (2012):82,
https://hdl.handle.net/21.15107/rcub_cer_5278 .

TY  - JOUR
AU  - Martinov, Olga B.
AU  - Spasić, Snežana
AU  - Lugonja, Nikoleta
AU  - Gojgić-Cvijović, Gordana
AU  - Vrvić, Miroslav
PY  - 2011
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/913
AB  - Six commercially available infant formulas (IF) without added prebiotic, were investigated as substrates for the growth of bifidobacteria isolated from the feces of 3 day old babies. This investigation tracked, during 48 h, the microbiological and biochemical changes in prepared infant formula meals, caused by the action of bifidobacteria, isolated from babies' feces. Pancreatin digestion preceded inoculation of the bifidobacteria biomass. The reference substrate was mature breast milk. This investigation showed important differences in bifidogenic effect between individual infant formulas and mature breast milk. In relation to dry biomass from the mature breast milk, dry biomass obtained in individual infant formulas ranged from 71.4% (IF-1) to 34.6% (IF-6). The results showed that, with the similar declared nutritive and biological characteristics, there are important differences between individual infant formulas. The results obtained showed that, an in vitro bifidogenic index test which covers tracking of microbiological and biochemical changes should be developed as a usefull tool in determining the suitability of infant formulas and it should be one of the first steps before clinical investigations.
PB  - Academic Journals, Victoria Island
T2  - African Journal of Biotechnology
T1  - Infant formula as a substrate for bifidogenesis: An in vitro investigation
VL  - 10
IS  - 12
SP  - 2302
EP  - 2307
DO  - 10.5897/AJB10.580
ER  - 

@article{
author = "Martinov, Olga B. and Spasić, Snežana and Lugonja, Nikoleta and Gojgić-Cvijović, Gordana and Vrvić, Miroslav",
year = "2011",
abstract = "Six commercially available infant formulas (IF) without added prebiotic, were investigated as substrates for the growth of bifidobacteria isolated from the feces of 3 day old babies. This investigation tracked, during 48 h, the microbiological and biochemical changes in prepared infant formula meals, caused by the action of bifidobacteria, isolated from babies' feces. Pancreatin digestion preceded inoculation of the bifidobacteria biomass. The reference substrate was mature breast milk. This investigation showed important differences in bifidogenic effect between individual infant formulas and mature breast milk. In relation to dry biomass from the mature breast milk, dry biomass obtained in individual infant formulas ranged from 71.4% (IF-1) to 34.6% (IF-6). The results showed that, with the similar declared nutritive and biological characteristics, there are important differences between individual infant formulas. The results obtained showed that, an in vitro bifidogenic index test which covers tracking of microbiological and biochemical changes should be developed as a usefull tool in determining the suitability of infant formulas and it should be one of the first steps before clinical investigations.",
publisher = "Academic Journals, Victoria Island",
journal = "African Journal of Biotechnology",
title = "Infant formula as a substrate for bifidogenesis: An in vitro investigation",
volume = "10",
number = "12",
pages = "2302-2307",
doi = "10.5897/AJB10.580"
}

Martinov, O. B., Spasić, S., Lugonja, N., Gojgić-Cvijović, G.,& Vrvić, M.. (2011). Infant formula as a substrate for bifidogenesis: An in vitro investigation. in African Journal of Biotechnology
Academic Journals, Victoria Island., 10(12), 2302-2307.
https://doi.org/10.5897/AJB10.580

Martinov OB, Spasić S, Lugonja N, Gojgić-Cvijović G, Vrvić M. Infant formula as a substrate for bifidogenesis: An in vitro investigation. in African Journal of Biotechnology. 2011;10(12):2302-2307.
doi:10.5897/AJB10.580 .

Martinov, Olga B., Spasić, Snežana, Lugonja, Nikoleta, Gojgić-Cvijović, Gordana, Vrvić, Miroslav, "Infant formula as a substrate for bifidogenesis: An in vitro investigation" in African Journal of Biotechnology, 10, no. 12 (2011):2302-2307,
https://doi.org/10.5897/AJB10.580 . .

TY  - JOUR
AU  - Ilić, Mila
AU  - Antić, Mališa
AU  - Antić, Vesna
AU  - Schwarzbauer, Jan
AU  - Vrvić, Miroslav
AU  - Jovančićević, Branimir
PY  - 2011
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/889
AB  - Bioremediation potential of bacteria and fungi isolated from sludge samples has been investigated (Danube alluvium, Panevo, Serbia). Total isolated microorganisms were divided into three parts. One part was added with actidione antifungicide. The second part was added with streptomycin antibiotic. The third part was without additives. Paraffinic type of crude oil was a substrate for assessment of bioremediation potential. The simulated oil biodegradation lasted 30, 60 and 90 days. Parallel with that, the experiments with blind trial were conducted. Extracts were isolated from the samples with chloroform in a separate funnel. They were assayed for the group composition (alkanes, aromatics, alcohols and fatty acids) by column chromatography. Alkane fraction was analysed by gas chromatography-mass spectrometry (GC-MS). The most intense oil degradation was achieved in the experiments with bacteria, somewhat weaker with consortium of fungi and bacteria, and the weakest bioremediation potential in these experiments was shown by fungi.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Chemistry Letters
T1  - Investigation of bioremediation potential of zymogenous bacteria and fungi for crude oil degradation
VL  - 9
IS  - 1
SP  - 133
EP  - 140
DO  - 10.1007/s10311-009-0257-3
ER  - 

@article{
author = "Ilić, Mila and Antić, Mališa and Antić, Vesna and Schwarzbauer, Jan and Vrvić, Miroslav and Jovančićević, Branimir",
year = "2011",
abstract = "Bioremediation potential of bacteria and fungi isolated from sludge samples has been investigated (Danube alluvium, Panevo, Serbia). Total isolated microorganisms were divided into three parts. One part was added with actidione antifungicide. The second part was added with streptomycin antibiotic. The third part was without additives. Paraffinic type of crude oil was a substrate for assessment of bioremediation potential. The simulated oil biodegradation lasted 30, 60 and 90 days. Parallel with that, the experiments with blind trial were conducted. Extracts were isolated from the samples with chloroform in a separate funnel. They were assayed for the group composition (alkanes, aromatics, alcohols and fatty acids) by column chromatography. Alkane fraction was analysed by gas chromatography-mass spectrometry (GC-MS). The most intense oil degradation was achieved in the experiments with bacteria, somewhat weaker with consortium of fungi and bacteria, and the weakest bioremediation potential in these experiments was shown by fungi.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Chemistry Letters",
title = "Investigation of bioremediation potential of zymogenous bacteria and fungi for crude oil degradation",
volume = "9",
number = "1",
pages = "133-140",
doi = "10.1007/s10311-009-0257-3"
}

Ilić, M., Antić, M., Antić, V., Schwarzbauer, J., Vrvić, M.,& Jovančićević, B.. (2011). Investigation of bioremediation potential of zymogenous bacteria and fungi for crude oil degradation. in Environmental Chemistry Letters
Springer Heidelberg, Heidelberg., 9(1), 133-140.
https://doi.org/10.1007/s10311-009-0257-3

Ilić M, Antić M, Antić V, Schwarzbauer J, Vrvić M, Jovančićević B. Investigation of bioremediation potential of zymogenous bacteria and fungi for crude oil degradation. in Environmental Chemistry Letters. 2011;9(1):133-140.
doi:10.1007/s10311-009-0257-3 .

Ilić, Mila, Antić, Mališa, Antić, Vesna, Schwarzbauer, Jan, Vrvić, Miroslav, Jovančićević, Branimir, "Investigation of bioremediation potential of zymogenous bacteria and fungi for crude oil degradation" in Environmental Chemistry Letters, 9, no. 1 (2011):133-140,
https://doi.org/10.1007/s10311-009-0257-3 . .

TY  - JOUR
AU  - Beškoski, Vladimir
AU  - Gojgić-Cvijović, Gordana
AU  - Milić, Jelena
AU  - Ilić, Mila
AU  - Miletić, Srđan
AU  - Šolević Knudsen, Tatjana
AU  - Vrvić, Miroslav
PY  - 2011
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/904
AB  - Mazut (heavy residual fuel oil)-polluted soil was exposed to bioremediation in an ex situ field-scale (600 m(3)) study. Re-inoculation was performed periodically with biomasses of microbial consortia isolated from the mazut-contaminated soil. Biostimulation was conducted by adding nutritional elements (N, P and K). The biopile (depth 0.4 m) was comprised of mechanically mixed polluted soil with softwood sawdust and crude river sand. Aeration was improved by systematic mixing. The biopile was protected from direct external influences by a polyethylene cover. Part (10 m3) of the material prepared for bioremediation was set aside uninoculated, and maintained as an untreated control pile (CP). Biostimulation and re-inoculation with zymogenous microorganisms increased the number of hydrocarbon degraders after 50 d by more than 20 times in the treated soil. During the 5 months, the total petroleum hydrocarbon (TPH) content of the contaminated soil was reduced to 6% of the initial value, from 5.2 to 0.3 g kg(-1) dry matter, while TPH reduced to only 90% of the initial value in the CP. After 150 d there were 96%, 97% and 83% reductions for the aliphatic, aromatic, and nitrogen-sulphur-oxygen and asphaltene fractions, respectively. The isoprenoids, pristane and phytane, were more than 55% biodegraded, which indicated that they are not suitable biomarkers for following bioremediation. According to the available data, this is the first field-scale study of the bioremediation of mazut and mazut sediment-polluted soil, and the efficiency achieved was far above that described in the literature to date for heavy fuel oil.
PB  - Oxford : Pergamon-Elsevier Science Ltd
T2  - Chemosphere
T1  - Ex situ bioremediation of a soil contaminated by mazut (heavy residual fuel oil) - A field experiment
VL  - 83
IS  - 1
SP  - 34
EP  - 40
DO  - 10.1016/j.chemosphere.2011.01.020
ER  - 

@article{
author = "Beškoski, Vladimir and Gojgić-Cvijović, Gordana and Milić, Jelena and Ilić, Mila and Miletić, Srđan and Šolević Knudsen, Tatjana and Vrvić, Miroslav",
year = "2011",
abstract = "Mazut (heavy residual fuel oil)-polluted soil was exposed to bioremediation in an ex situ field-scale (600 m(3)) study. Re-inoculation was performed periodically with biomasses of microbial consortia isolated from the mazut-contaminated soil. Biostimulation was conducted by adding nutritional elements (N, P and K). The biopile (depth 0.4 m) was comprised of mechanically mixed polluted soil with softwood sawdust and crude river sand. Aeration was improved by systematic mixing. The biopile was protected from direct external influences by a polyethylene cover. Part (10 m3) of the material prepared for bioremediation was set aside uninoculated, and maintained as an untreated control pile (CP). Biostimulation and re-inoculation with zymogenous microorganisms increased the number of hydrocarbon degraders after 50 d by more than 20 times in the treated soil. During the 5 months, the total petroleum hydrocarbon (TPH) content of the contaminated soil was reduced to 6% of the initial value, from 5.2 to 0.3 g kg(-1) dry matter, while TPH reduced to only 90% of the initial value in the CP. After 150 d there were 96%, 97% and 83% reductions for the aliphatic, aromatic, and nitrogen-sulphur-oxygen and asphaltene fractions, respectively. The isoprenoids, pristane and phytane, were more than 55% biodegraded, which indicated that they are not suitable biomarkers for following bioremediation. According to the available data, this is the first field-scale study of the bioremediation of mazut and mazut sediment-polluted soil, and the efficiency achieved was far above that described in the literature to date for heavy fuel oil.",
publisher = "Oxford : Pergamon-Elsevier Science Ltd",
journal = "Chemosphere",
title = "Ex situ bioremediation of a soil contaminated by mazut (heavy residual fuel oil) - A field experiment",
volume = "83",
number = "1",
pages = "34-40",
doi = "10.1016/j.chemosphere.2011.01.020"
}

Beškoski, V., Gojgić-Cvijović, G., Milić, J., Ilić, M., Miletić, S., Šolević Knudsen, T.,& Vrvić, M.. (2011). Ex situ bioremediation of a soil contaminated by mazut (heavy residual fuel oil) - A field experiment. in Chemosphere
Oxford : Pergamon-Elsevier Science Ltd., 83(1), 34-40.
https://doi.org/10.1016/j.chemosphere.2011.01.020

Beškoski V, Gojgić-Cvijović G, Milić J, Ilić M, Miletić S, Šolević Knudsen T, Vrvić M. Ex situ bioremediation of a soil contaminated by mazut (heavy residual fuel oil) - A field experiment. in Chemosphere. 2011;83(1):34-40.
doi:10.1016/j.chemosphere.2011.01.020 .

Beškoski, Vladimir, Gojgić-Cvijović, Gordana, Milić, Jelena, Ilić, Mila, Miletić, Srđan, Šolević Knudsen, Tatjana, Vrvić, Miroslav, "Ex situ bioremediation of a soil contaminated by mazut (heavy residual fuel oil) - A field experiment" in Chemosphere, 83, no. 1 (2011):34-40,
https://doi.org/10.1016/j.chemosphere.2011.01.020 . .

TY  - CONF
AU  - Beškoski, Vladimir
AU  - Gojgić-Cvijović, Gordana
AU  - Ilić, Mila
AU  - Milić, Jelena
AU  - Miletić, Srđan
PY  - 2011
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5010
AB  - Potreba za ozdravljenjem i očišćenjem životne sredine je nesumnjiva i velika. Višedecenijski nemar i nečinjenje, ali i drugi razlozi, doveli su do postojanja velikog broja lokaliteta kontaminiranih različitim zagađujućim supstancama. Jedna od tehnologija koja beleži izuzetan uspeh u svetu kada je u pitanju saniranje zagađenja poreklom od nafte je i bioremedijacija.
PB  - Beograd : Forum kvalteta
C3  - Zbornik radova Šeste regionalne naučno-stručne konferencije o sistemu upravljanja zaštitom životne sredine u elektroprivredi, 06-10. decembar 2010., Zlatibor
T1  - Bioremedijacija u sistemu upravljanja zaštitom životne sredinenaša iskustva i primeri
SP  - 228
EP  - 238
UR  - https://hdl.handle.net/21.15107/rcub_cer_5010
ER  - 

@conference{
author = "Beškoski, Vladimir and Gojgić-Cvijović, Gordana and Ilić, Mila and Milić, Jelena and Miletić, Srđan",
year = "2011",
abstract = "Potreba za ozdravljenjem i očišćenjem životne sredine je nesumnjiva i velika. Višedecenijski nemar i nečinjenje, ali i drugi razlozi, doveli su do postojanja velikog broja lokaliteta kontaminiranih različitim zagađujućim supstancama. Jedna od tehnologija koja beleži izuzetan uspeh u svetu kada je u pitanju saniranje zagađenja poreklom od nafte je i bioremedijacija.",
publisher = "Beograd : Forum kvalteta",
journal = "Zbornik radova Šeste regionalne naučno-stručne konferencije o sistemu upravljanja zaštitom životne sredine u elektroprivredi, 06-10. decembar 2010., Zlatibor",
title = "Bioremedijacija u sistemu upravljanja zaštitom životne sredinenaša iskustva i primeri",
pages = "228-238",
url = "https://hdl.handle.net/21.15107/rcub_cer_5010"
}

Beškoski, V., Gojgić-Cvijović, G., Ilić, M., Milić, J.,& Miletić, S.. (2011). Bioremedijacija u sistemu upravljanja zaštitom životne sredinenaša iskustva i primeri. in Zbornik radova Šeste regionalne naučno-stručne konferencije o sistemu upravljanja zaštitom životne sredine u elektroprivredi, 06-10. decembar 2010., Zlatibor
Beograd : Forum kvalteta., 228-238.
https://hdl.handle.net/21.15107/rcub_cer_5010

Beškoski V, Gojgić-Cvijović G, Ilić M, Milić J, Miletić S. Bioremedijacija u sistemu upravljanja zaštitom životne sredinenaša iskustva i primeri. in Zbornik radova Šeste regionalne naučno-stručne konferencije o sistemu upravljanja zaštitom životne sredine u elektroprivredi, 06-10. decembar 2010., Zlatibor. 2011;:228-238.
https://hdl.handle.net/21.15107/rcub_cer_5010 .

Beškoski, Vladimir, Gojgić-Cvijović, Gordana, Ilić, Mila, Milić, Jelena, Miletić, Srđan, "Bioremedijacija u sistemu upravljanja zaštitom životne sredinenaša iskustva i primeri" in Zbornik radova Šeste regionalne naučno-stručne konferencije o sistemu upravljanja zaštitom životne sredine u elektroprivredi, 06-10. decembar 2010., Zlatibor (2011):228-238,
https://hdl.handle.net/21.15107/rcub_cer_5010 .

TY  - JOUR
AU  - Šolević Knudsen, Tatjana
AU  - Novakovic, Milan
AU  - Ilić, Mila
AU  - Antić, Mališa
AU  - Vrvić, Miroslav
AU  - Jovančićević, Branimir
PY  - 2011
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/909
AB  - The bioremediation potential of the aerobic zymogenous microorganisms in soil (Danube alluvium, Pančevo, Serbia) for crude oil biodegradation was investigated. A mixture of paraffinic types of oils was used as the substrate. The laboratory experiment of the simulated oil biodegradation lasted 15, 30, 45, 60 and 75 days. In parallel, an experiment with a control sample was conducted. Extracts were isolated from the samples with chloroform in a separation funnel. From these extracts, the hydrocarbons were isolated by column chromatography and analyzed by gas chromatography-mass spectrometry (GC-MS). n-Alkanes, isoprenoids, phenanthrene and its derivatives with one and two methyl groups were quantitatively analyzed. The ability and efficiency of zymogenous microorganisms in soil for crude oil bioremediation was assessed by comparison between the composition of samples which were exposed to the microorganisms and the control sample. The investigated microorganisms showed the highest bioremediation potential in the biodegradation of n-alkanes and isoprenoids. A considerably high bioremediation potential was confirmed in the biodegradation of phenanthrene and methyl phenanthrenes. Low bioremediation potential of these microorganisms was proven in the case of polycyclic alkanes of the sterane and triterpane types and dimethyl phenanthrenes.
AB  - Ispitivan je bioremedijacioni potencijal aerobnih zimogenih mikroorganizama iz zemljišta u biodegradaciji sirove nafte (aluvijalna ravan reke Dunav, Pančevo). Smeša sirovih nafti parafinskog tipa korišćena je kao supstrat. Laboratorijski eksperiment simulirane biodegradacije trajao je 15, 30, 45, 60 i 75 dana. Paralelno je rađen i eksperiment sa kontrolnim uzorkom. Ekstrakti su izolovani iz uzoraka hloroformom u levku za odvajanje. Iz ovih ekstrakata, ugljovodonici su izolovani hromatografijom na koloni i analizirani gasnohromatografski-masenospektrometrijski (GC-MS). n-Alkani, izoprenoidi, fenantren i njegovi derivati sa jednom i dve metil grupe kvantitativno su analizirani. Sposobnost i efikasnost zimogenih mikroorganizama iz zemljišta u biodegradaciji sirove nafte procenjena je poređenjem sastava uzoraka koji su bili izloženi mikroorganizmima i kontrolnog uzorka. Ispitivani mikroorganizmi pokazali su najviši bioremedijacioni potencijal u biodegradaciji n-alkana i izoprenoida. Visok biodegradacioni potencijal uočen je pri biodegradaciji fenantrena i metilfenantrena. Nizak bioremedijacioni potencijal ovih mikroorganizma dokazan je u slučaju policikličnih alkana tipa sterana i terpana, kao i dimetilfenantrena.
PB  - Serbian Chemical Soc, Belgrade
T2  - Journal of the Serbian Chemical Society
T1  - Investigation of the bioremediation potential of aerobic zymogenous microorganisms in soil for crude oil biodegradation
T1  - Ispitivanje bioremedijacionog potencijala aerobnih zimogenih mikroorganizama iz zemljišta u biodegradaciji sirove nafte
VL  - 76
IS  - 3
SP  - 425
EP  - 438
DO  - 10.2298/JSC100531033S
ER  - 

@article{
author = "Šolević Knudsen, Tatjana and Novakovic, Milan and Ilić, Mila and Antić, Mališa and Vrvić, Miroslav and Jovančićević, Branimir",
year = "2011",
abstract = "The bioremediation potential of the aerobic zymogenous microorganisms in soil (Danube alluvium, Pančevo, Serbia) for crude oil biodegradation was investigated. A mixture of paraffinic types of oils was used as the substrate. The laboratory experiment of the simulated oil biodegradation lasted 15, 30, 45, 60 and 75 days. In parallel, an experiment with a control sample was conducted. Extracts were isolated from the samples with chloroform in a separation funnel. From these extracts, the hydrocarbons were isolated by column chromatography and analyzed by gas chromatography-mass spectrometry (GC-MS). n-Alkanes, isoprenoids, phenanthrene and its derivatives with one and two methyl groups were quantitatively analyzed. The ability and efficiency of zymogenous microorganisms in soil for crude oil bioremediation was assessed by comparison between the composition of samples which were exposed to the microorganisms and the control sample. The investigated microorganisms showed the highest bioremediation potential in the biodegradation of n-alkanes and isoprenoids. A considerably high bioremediation potential was confirmed in the biodegradation of phenanthrene and methyl phenanthrenes. Low bioremediation potential of these microorganisms was proven in the case of polycyclic alkanes of the sterane and triterpane types and dimethyl phenanthrenes., Ispitivan je bioremedijacioni potencijal aerobnih zimogenih mikroorganizama iz zemljišta u biodegradaciji sirove nafte (aluvijalna ravan reke Dunav, Pančevo). Smeša sirovih nafti parafinskog tipa korišćena je kao supstrat. Laboratorijski eksperiment simulirane biodegradacije trajao je 15, 30, 45, 60 i 75 dana. Paralelno je rađen i eksperiment sa kontrolnim uzorkom. Ekstrakti su izolovani iz uzoraka hloroformom u levku za odvajanje. Iz ovih ekstrakata, ugljovodonici su izolovani hromatografijom na koloni i analizirani gasnohromatografski-masenospektrometrijski (GC-MS). n-Alkani, izoprenoidi, fenantren i njegovi derivati sa jednom i dve metil grupe kvantitativno su analizirani. Sposobnost i efikasnost zimogenih mikroorganizama iz zemljišta u biodegradaciji sirove nafte procenjena je poređenjem sastava uzoraka koji su bili izloženi mikroorganizmima i kontrolnog uzorka. Ispitivani mikroorganizmi pokazali su najviši bioremedijacioni potencijal u biodegradaciji n-alkana i izoprenoida. Visok biodegradacioni potencijal uočen je pri biodegradaciji fenantrena i metilfenantrena. Nizak bioremedijacioni potencijal ovih mikroorganizma dokazan je u slučaju policikličnih alkana tipa sterana i terpana, kao i dimetilfenantrena.",
publisher = "Serbian Chemical Soc, Belgrade",
journal = "Journal of the Serbian Chemical Society",
title = "Investigation of the bioremediation potential of aerobic zymogenous microorganisms in soil for crude oil biodegradation, Ispitivanje bioremedijacionog potencijala aerobnih zimogenih mikroorganizama iz zemljišta u biodegradaciji sirove nafte",
volume = "76",
number = "3",
pages = "425-438",
doi = "10.2298/JSC100531033S"
}

Šolević Knudsen, T., Novakovic, M., Ilić, M., Antić, M., Vrvić, M.,& Jovančićević, B.. (2011). Investigation of the bioremediation potential of aerobic zymogenous microorganisms in soil for crude oil biodegradation. in Journal of the Serbian Chemical Society
Serbian Chemical Soc, Belgrade., 76(3), 425-438.
https://doi.org/10.2298/JSC100531033S

Šolević Knudsen T, Novakovic M, Ilić M, Antić M, Vrvić M, Jovančićević B. Investigation of the bioremediation potential of aerobic zymogenous microorganisms in soil for crude oil biodegradation. in Journal of the Serbian Chemical Society. 2011;76(3):425-438.
doi:10.2298/JSC100531033S .

Šolević Knudsen, Tatjana, Novakovic, Milan, Ilić, Mila, Antić, Mališa, Vrvić, Miroslav, Jovančićević, Branimir, "Investigation of the bioremediation potential of aerobic zymogenous microorganisms in soil for crude oil biodegradation" in Journal of the Serbian Chemical Society, 76, no. 3 (2011):425-438,
https://doi.org/10.2298/JSC100531033S . .

TY  - CONF
AU  - Beškoski, Vladimir
AU  - Gojgić-Cvijović, Gordana
AU  - Milić, Jelena
AU  - Ilić, Mila
AU  - Miletić, Srđan
AU  - Spasić, Snežana
AU  - Takić, Miloš
AU  - Šolević Knudsen, Tatjana
AU  - Šainović, Aleksandra
AU  - Vučković, Ivan
AU  - Jovančićević, Branimir
AU  - Vrvić, Miroslav
PY  - 2010
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5455
AB  - This paper presents a review of three bioremediation procedures that our research team has successfully implemented on the industrial level. By bioremediation we have processed the soil con¬taminated by crude oil and oil derivatives, then off-balance mazut (masout) and finally, waste emulsions from metal processing industry by applying a multi-stage procedure followed up by bioremediation.
AB  - U ovom radu dat je pregled tri bioremedijaciona postupka koje je naga istraživačka grupa uspešno realizovala na industrijskom nivou. Bioremedijacijom su obradeni zemlja kontaminirana sirovom naftom i naftnim derivatima, potom vanbilansni mazut, a višestepenim postupkom, koji uključuje bioremedijaciju i otpadne emulzije iz metalnoprerađivačke industrije.
PB  - Beograd : IHTM, Centar za remedijaciju
C3  - Proceedings - The 3rd International Scientific Conference „Remediation 2010“ - Contemporary Remediation Technologies for Environmental Conservation and Protection
T1  - Ex situ bioremediation in mobile facility: Our experience with mobile production bioreactor for microbial biomass and various types and levels of pollutants
SP  - 1
EP  - 9
UR  - https://hdl.handle.net/21.15107/rcub_cer_5455
ER  - 

@conference{
author = "Beškoski, Vladimir and Gojgić-Cvijović, Gordana and Milić, Jelena and Ilić, Mila and Miletić, Srđan and Spasić, Snežana and Takić, Miloš and Šolević Knudsen, Tatjana and Šainović, Aleksandra and Vučković, Ivan and Jovančićević, Branimir and Vrvić, Miroslav",
year = "2010",
abstract = "This paper presents a review of three bioremediation procedures that our research team has successfully implemented on the industrial level. By bioremediation we have processed the soil con¬taminated by crude oil and oil derivatives, then off-balance mazut (masout) and finally, waste emulsions from metal processing industry by applying a multi-stage procedure followed up by bioremediation., U ovom radu dat je pregled tri bioremedijaciona postupka koje je naga istraživačka grupa uspešno realizovala na industrijskom nivou. Bioremedijacijom su obradeni zemlja kontaminirana sirovom naftom i naftnim derivatima, potom vanbilansni mazut, a višestepenim postupkom, koji uključuje bioremedijaciju i otpadne emulzije iz metalnoprerađivačke industrije.",
publisher = "Beograd : IHTM, Centar za remedijaciju",
journal = "Proceedings - The 3rd International Scientific Conference „Remediation 2010“ - Contemporary Remediation Technologies for Environmental Conservation and Protection",
title = "Ex situ bioremediation in mobile facility: Our experience with mobile production bioreactor for microbial biomass and various types and levels of pollutants",
pages = "1-9",
url = "https://hdl.handle.net/21.15107/rcub_cer_5455"
}

Beškoski, V., Gojgić-Cvijović, G., Milić, J., Ilić, M., Miletić, S., Spasić, S., Takić, M., Šolević Knudsen, T., Šainović, A., Vučković, I., Jovančićević, B.,& Vrvić, M.. (2010). Ex situ bioremediation in mobile facility: Our experience with mobile production bioreactor for microbial biomass and various types and levels of pollutants. in Proceedings - The 3rd International Scientific Conference „Remediation 2010“ - Contemporary Remediation Technologies for Environmental Conservation and Protection
Beograd : IHTM, Centar za remedijaciju., 1-9.
https://hdl.handle.net/21.15107/rcub_cer_5455

Beškoski V, Gojgić-Cvijović G, Milić J, Ilić M, Miletić S, Spasić S, Takić M, Šolević Knudsen T, Šainović A, Vučković I, Jovančićević B, Vrvić M. Ex situ bioremediation in mobile facility: Our experience with mobile production bioreactor for microbial biomass and various types and levels of pollutants. in Proceedings - The 3rd International Scientific Conference „Remediation 2010“ - Contemporary Remediation Technologies for Environmental Conservation and Protection. 2010;:1-9.
https://hdl.handle.net/21.15107/rcub_cer_5455 .

Beškoski, Vladimir, Gojgić-Cvijović, Gordana, Milić, Jelena, Ilić, Mila, Miletić, Srđan, Spasić, Snežana, Takić, Miloš, Šolević Knudsen, Tatjana, Šainović, Aleksandra, Vučković, Ivan, Jovančićević, Branimir, Vrvić, Miroslav, "Ex situ bioremediation in mobile facility: Our experience with mobile production bioreactor for microbial biomass and various types and levels of pollutants" in Proceedings - The 3rd International Scientific Conference „Remediation 2010“ - Contemporary Remediation Technologies for Environmental Conservation and Protection (2010):1-9,
https://hdl.handle.net/21.15107/rcub_cer_5455 .

TY  - JOUR
AU  - Lugonja, Nikoleta
AU  - Martinov, Olga B.
AU  - Rasovic, Mirjana R.
AU  - Spasić, Snežana
AU  - Gojgić-Cvijović, Gordana
AU  - Vrvić, Miroslav
PY  - 2010
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/688
AB  - The bifidogenic effect of an infant formula supplemented with inulin and fructooligosaccharides (4.0 g/l) was examined clinically and in vitro, and compared that of mature breast milk. In a 28-day clinical study, fecal samples of 21 infants, divided into two groups: one receiving the infant formula and the other breast milk, were microbiologically and biochemically examined. In the in vitro investigation, microbiological and biochemical changes in the infant formula and breast milk induced by the action of bifidobacteria isolated from infant feces were examined. There were no significant differences in the fecal numbers of lactobacilli, total aerobes, anaerobes or yeasts and fungi. In contrast, the bifidobacteria numbers in the stools increased significantly during the study in the infants receiving the supplemented formula. The comparative in vitro test showed that the bifidogenic effect was similar for infant formula and breast milk in terms of the number of bifidobacteria. Consumption of infant formula with added inulin and fructooligosaccharides stimulated the bifidogenic effect, both clinically and in vitro. The in vitro test can quickly and objectively determine the bifidogenic effect of infant formula and indicate their quality. However, a clinical test is necessary to determine the acceptance and biological value of infant formula.
PB  - Journal Clinical Biochemistry & Nutrition, Kyoto
T2  - Journal of Clinical Biochemistry and Nutrition
T1  - A Comparative Investigation of an in vitro and Clinical Test of the Bifidogenic Effect of an Infant Formula
VL  - 47
IS  - 3
SP  - 208
EP  - 216
DO  - 10.3164/jcbn.10-54
ER  - 

@article{
author = "Lugonja, Nikoleta and Martinov, Olga B. and Rasovic, Mirjana R. and Spasić, Snežana and Gojgić-Cvijović, Gordana and Vrvić, Miroslav",
year = "2010",
abstract = "The bifidogenic effect of an infant formula supplemented with inulin and fructooligosaccharides (4.0 g/l) was examined clinically and in vitro, and compared that of mature breast milk. In a 28-day clinical study, fecal samples of 21 infants, divided into two groups: one receiving the infant formula and the other breast milk, were microbiologically and biochemically examined. In the in vitro investigation, microbiological and biochemical changes in the infant formula and breast milk induced by the action of bifidobacteria isolated from infant feces were examined. There were no significant differences in the fecal numbers of lactobacilli, total aerobes, anaerobes or yeasts and fungi. In contrast, the bifidobacteria numbers in the stools increased significantly during the study in the infants receiving the supplemented formula. The comparative in vitro test showed that the bifidogenic effect was similar for infant formula and breast milk in terms of the number of bifidobacteria. Consumption of infant formula with added inulin and fructooligosaccharides stimulated the bifidogenic effect, both clinically and in vitro. The in vitro test can quickly and objectively determine the bifidogenic effect of infant formula and indicate their quality. However, a clinical test is necessary to determine the acceptance and biological value of infant formula.",
publisher = "Journal Clinical Biochemistry & Nutrition, Kyoto",
journal = "Journal of Clinical Biochemistry and Nutrition",
title = "A Comparative Investigation of an in vitro and Clinical Test of the Bifidogenic Effect of an Infant Formula",
volume = "47",
number = "3",
pages = "208-216",
doi = "10.3164/jcbn.10-54"
}

Lugonja, N., Martinov, O. B., Rasovic, M. R., Spasić, S., Gojgić-Cvijović, G.,& Vrvić, M.. (2010). A Comparative Investigation of an in vitro and Clinical Test of the Bifidogenic Effect of an Infant Formula. in Journal of Clinical Biochemistry and Nutrition
Journal Clinical Biochemistry & Nutrition, Kyoto., 47(3), 208-216.
https://doi.org/10.3164/jcbn.10-54

Lugonja N, Martinov OB, Rasovic MR, Spasić S, Gojgić-Cvijović G, Vrvić M. A Comparative Investigation of an in vitro and Clinical Test of the Bifidogenic Effect of an Infant Formula. in Journal of Clinical Biochemistry and Nutrition. 2010;47(3):208-216.
doi:10.3164/jcbn.10-54 .

Lugonja, Nikoleta, Martinov, Olga B., Rasovic, Mirjana R., Spasić, Snežana, Gojgić-Cvijović, Gordana, Vrvić, Miroslav, "A Comparative Investigation of an in vitro and Clinical Test of the Bifidogenic Effect of an Infant Formula" in Journal of Clinical Biochemistry and Nutrition, 47, no. 3 (2010):208-216,
https://doi.org/10.3164/jcbn.10-54 . .

TY  - JOUR
AU  - Beškoski, Vladimir
AU  - Takic, Milos
AU  - Milić, Jelena
AU  - Ilić, Mila
AU  - Gojgić-Cvijović, Gordana
AU  - Jovančićević, Branimir
AU  - Vrvić, Miroslav
PY  - 2010
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/686
AB  - This paper presents the results of an ex situ bioremediation of soil contaminated by mazut (heavy residual fuel oil) in the field scale (600 m3). The treatment-bed (thickness 0.4 m) consisted of mechanically mixed mazut-contaminated soil, softwood sawdust as an additional carbon source and crude river sand, as a bulking and porosity increasing material. The inoculation/rein-oculation was conducted periodically using a biomass of a consortium of zymogenous microorganisms isolated from a bioremediation substrate. The biostimulation was performed through addition of nutritious substances (N, P and K). The aeration was improved by systematic mixing of the bioremediation system. After 50 days, the number of hydrocarbon degraders had increased a 100 fold. Based on the changes in the group composition, the average biodegradation rate during bioremediation was 24 mg kg-1 day-1 for the aliphatic fraction, 6 mg kg-1 day-1 for the aromatic fraction and 3 mg kg-1 day-1 for the nitrogen-sulphur-oxygen compounds (NSO)-asphaltene fraction. In the saturated hydrocarbon fraction, gas chromatography-mass spectrometry (GC-MS) in the single ion-monitoring mode (SIM) was applied to analyze isoprenoids pristane and phytane and polycyclic molecules of sterane and triterpane type. Biodegradation occurred during the bioremediation process, as well as a reduction of the relative quantities of isoprenoids, steranes, tri- and tetracyclic terpanes and pentacyclic terpanes of the hopane type.
AB  - Prikazani su rezultati ex situ bioremedijacije zemljišta kontaminiranog mazutom na industrijskom nivou (600 m3). Bioremedijacioni materijal (debljine 0,4 m) se sastojao od mehanički pomešanih mazutom zagađenog zemljišta, čamove piljevine kao dodatnog izvora ugljenika i neprečišćenog rečnog peska, dodatog u cilju mešanja i povećanja poroznosti. Inokulacija/reinokulacija (bioaugmentacija) je periodično rađena sa biomasom konzorcijuma zimogenih mikrooorganizama izolovanih iz supstrata za bioremedijaciju. Biostimulacija je realizovana dodatkom hranljivih supstanci (N, P i K). Aeracija je poboljšavana sistematskim mešanjem bioremedijacionog sistema. Nakon 50 dana broj mikroorganizama koji degradiraju ugljovodonike povećan je 100 puta. Na osnovu promena u grupnom sastavu prosečna stopa biodegradacije tokom bioremedijacije je bila za alifatičnu frakciju 24 mg kg-1 po danu, za aromatičnu 6 mg kg-1 po danu i 3 mg kg-1 po danu za NSO-asfaltensku frakciju. U zasićenoj ugljovodoničnoj frakciji metodom GC-MS (SIM metod) analizirani su izoprenoidi pristan i fitan i policiklični molekuli steranskog i triterpanskog tipa. Tokom bioremedijacionog procesa došlo je do biodegradacije i smanjenja relativnih količina izoprenoida, sterana, tri- i tetracikličnih terpana i pentacikličnih terpana hopanskog tipa.
PB  - Serbian Chemical Soc, Belgrade
T2  - Journal of the Serbian Chemical Society
T1  - Change of isoprenoids, steranes and terpanes during ex situ bioremediation of mazut on the industrial scale
T1  - Promena izoprenoidne, steranske i terpanske frakcije tokom ex situ bioremedijacije mazuta na industrijskom nivou
VL  - 75
IS  - 11
SP  - 1605
EP  - 1616
DO  - 10.2298/JSC100505091B
ER  - 

@article{
author = "Beškoski, Vladimir and Takic, Milos and Milić, Jelena and Ilić, Mila and Gojgić-Cvijović, Gordana and Jovančićević, Branimir and Vrvić, Miroslav",
year = "2010",
abstract = "This paper presents the results of an ex situ bioremediation of soil contaminated by mazut (heavy residual fuel oil) in the field scale (600 m3). The treatment-bed (thickness 0.4 m) consisted of mechanically mixed mazut-contaminated soil, softwood sawdust as an additional carbon source and crude river sand, as a bulking and porosity increasing material. The inoculation/rein-oculation was conducted periodically using a biomass of a consortium of zymogenous microorganisms isolated from a bioremediation substrate. The biostimulation was performed through addition of nutritious substances (N, P and K). The aeration was improved by systematic mixing of the bioremediation system. After 50 days, the number of hydrocarbon degraders had increased a 100 fold. Based on the changes in the group composition, the average biodegradation rate during bioremediation was 24 mg kg-1 day-1 for the aliphatic fraction, 6 mg kg-1 day-1 for the aromatic fraction and 3 mg kg-1 day-1 for the nitrogen-sulphur-oxygen compounds (NSO)-asphaltene fraction. In the saturated hydrocarbon fraction, gas chromatography-mass spectrometry (GC-MS) in the single ion-monitoring mode (SIM) was applied to analyze isoprenoids pristane and phytane and polycyclic molecules of sterane and triterpane type. Biodegradation occurred during the bioremediation process, as well as a reduction of the relative quantities of isoprenoids, steranes, tri- and tetracyclic terpanes and pentacyclic terpanes of the hopane type., Prikazani su rezultati ex situ bioremedijacije zemljišta kontaminiranog mazutom na industrijskom nivou (600 m3). Bioremedijacioni materijal (debljine 0,4 m) se sastojao od mehanički pomešanih mazutom zagađenog zemljišta, čamove piljevine kao dodatnog izvora ugljenika i neprečišćenog rečnog peska, dodatog u cilju mešanja i povećanja poroznosti. Inokulacija/reinokulacija (bioaugmentacija) je periodično rađena sa biomasom konzorcijuma zimogenih mikrooorganizama izolovanih iz supstrata za bioremedijaciju. Biostimulacija je realizovana dodatkom hranljivih supstanci (N, P i K). Aeracija je poboljšavana sistematskim mešanjem bioremedijacionog sistema. Nakon 50 dana broj mikroorganizama koji degradiraju ugljovodonike povećan je 100 puta. Na osnovu promena u grupnom sastavu prosečna stopa biodegradacije tokom bioremedijacije je bila za alifatičnu frakciju 24 mg kg-1 po danu, za aromatičnu 6 mg kg-1 po danu i 3 mg kg-1 po danu za NSO-asfaltensku frakciju. U zasićenoj ugljovodoničnoj frakciji metodom GC-MS (SIM metod) analizirani su izoprenoidi pristan i fitan i policiklični molekuli steranskog i triterpanskog tipa. Tokom bioremedijacionog procesa došlo je do biodegradacije i smanjenja relativnih količina izoprenoida, sterana, tri- i tetracikličnih terpana i pentacikličnih terpana hopanskog tipa.",
publisher = "Serbian Chemical Soc, Belgrade",
journal = "Journal of the Serbian Chemical Society",
title = "Change of isoprenoids, steranes and terpanes during ex situ bioremediation of mazut on the industrial scale, Promena izoprenoidne, steranske i terpanske frakcije tokom ex situ bioremedijacije mazuta na industrijskom nivou",
volume = "75",
number = "11",
pages = "1605-1616",
doi = "10.2298/JSC100505091B"
}

Beškoski, V., Takic, M., Milić, J., Ilić, M., Gojgić-Cvijović, G., Jovančićević, B.,& Vrvić, M.. (2010). Change of isoprenoids, steranes and terpanes during ex situ bioremediation of mazut on the industrial scale. in Journal of the Serbian Chemical Society
Serbian Chemical Soc, Belgrade., 75(11), 1605-1616.
https://doi.org/10.2298/JSC100505091B

Beškoski V, Takic M, Milić J, Ilić M, Gojgić-Cvijović G, Jovančićević B, Vrvić M. Change of isoprenoids, steranes and terpanes during ex situ bioremediation of mazut on the industrial scale. in Journal of the Serbian Chemical Society. 2010;75(11):1605-1616.
doi:10.2298/JSC100505091B .

Beškoski, Vladimir, Takic, Milos, Milić, Jelena, Ilić, Mila, Gojgić-Cvijović, Gordana, Jovančićević, Branimir, Vrvić, Miroslav, "Change of isoprenoids, steranes and terpanes during ex situ bioremediation of mazut on the industrial scale" in Journal of the Serbian Chemical Society, 75, no. 11 (2010):1605-1616,
https://doi.org/10.2298/JSC100505091B . .

TY  - JOUR
AU  - Izrael‑Živković, Lidija
AU  - Gojgić-Cvijović, Gordana
AU  - Karadžić, Ivanka
PY  - 2010
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/713
AB  - Enzymatic characteristics of a protease from a medically important, referent strain of Pseudomonas aeruginosa ATCC 27853 were determined. According to sodium dodecyl sulfate polyacrylamide gel electrophoresis, SDS-PAGE, and gel filtration, it was estimated that the molecular mass of the purified enzyme was about 15 kDa. Other enzymatic properties were found to be: pH optimum 7.1, pH stability between 6.5 and 10; temperature optimum around 60 °C while the enzyme was stable at 60°C for 30 min. Inhibition of the enzyme was observed with metal chelators, such as EDTA and 1,10-phenanthroline, suggesting that the protease is a metalloenzyme. Furthermore, the enzyme contains one mole of zinc ion per mole of enzyme. The protease was stable in the presence of different organic solvents, which enables its potential use for the synthesis of peptides.
AB  - U ovom radu je okarakterisana ekstracelularna proteaza medicinski značajnog, referentnog soja Pseudomonas aeruginosa ATCC 27853. Molekulska masa prečišćenog enzima određena SDS PAGE i gel filtracijom iznosi oko 15 kDa. Određeni su sledeći enzimski parametri: pH optimum 7,1; pH stabilnost u opsegu 6,5-10; temperaturni optimum 60°C, a enzim je stabilan na 60°C 30 min. Na osnovu inhibicije enzima pomoću EDTA i 1,10-fenantrolina, utvrđeno je da proteaza predstavlja metaloenzim. Pokazano da proteaza sadrži 1 mol jona cinka po molu enzima. Proteaza je stabilna u prisustvu različitih organskih rastvarača, što omogućava upotrebu za sintezu peptida.
PB  - Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - Isolation and partial characterization of protease from Pseudomonas aeruginosa ATCC 27853
T1  - Izolovanje i delimično karakterisanje proteaze iz Pseudomonas aeruginosa ATCC 27853
VL  - 75
IS  - 8
SP  - 1041
EP  - 1052
DO  - 10.2298/JSC100125088I
ER  - 

@article{
author = "Izrael‑Živković, Lidija and Gojgić-Cvijović, Gordana and Karadžić, Ivanka",
year = "2010",
abstract = "Enzymatic characteristics of a protease from a medically important, referent strain of Pseudomonas aeruginosa ATCC 27853 were determined. According to sodium dodecyl sulfate polyacrylamide gel electrophoresis, SDS-PAGE, and gel filtration, it was estimated that the molecular mass of the purified enzyme was about 15 kDa. Other enzymatic properties were found to be: pH optimum 7.1, pH stability between 6.5 and 10; temperature optimum around 60 °C while the enzyme was stable at 60°C for 30 min. Inhibition of the enzyme was observed with metal chelators, such as EDTA and 1,10-phenanthroline, suggesting that the protease is a metalloenzyme. Furthermore, the enzyme contains one mole of zinc ion per mole of enzyme. The protease was stable in the presence of different organic solvents, which enables its potential use for the synthesis of peptides., U ovom radu je okarakterisana ekstracelularna proteaza medicinski značajnog, referentnog soja Pseudomonas aeruginosa ATCC 27853. Molekulska masa prečišćenog enzima određena SDS PAGE i gel filtracijom iznosi oko 15 kDa. Određeni su sledeći enzimski parametri: pH optimum 7,1; pH stabilnost u opsegu 6,5-10; temperaturni optimum 60°C, a enzim je stabilan na 60°C 30 min. Na osnovu inhibicije enzima pomoću EDTA i 1,10-fenantrolina, utvrđeno je da proteaza predstavlja metaloenzim. Pokazano da proteaza sadrži 1 mol jona cinka po molu enzima. Proteaza je stabilna u prisustvu različitih organskih rastvarača, što omogućava upotrebu za sintezu peptida.",
publisher = "Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Isolation and partial characterization of protease from Pseudomonas aeruginosa ATCC 27853, Izolovanje i delimično karakterisanje proteaze iz Pseudomonas aeruginosa ATCC 27853",
volume = "75",
number = "8",
pages = "1041-1052",
doi = "10.2298/JSC100125088I"
}

Izrael‑Živković, L., Gojgić-Cvijović, G.,& Karadžić, I.. (2010). Isolation and partial characterization of protease from Pseudomonas aeruginosa ATCC 27853. in Journal of the Serbian Chemical Society
Serbian Chemical Society., 75(8), 1041-1052.
https://doi.org/10.2298/JSC100125088I

Izrael‑Živković L, Gojgić-Cvijović G, Karadžić I. Isolation and partial characterization of protease from Pseudomonas aeruginosa ATCC 27853. in Journal of the Serbian Chemical Society. 2010;75(8):1041-1052.
doi:10.2298/JSC100125088I .

Izrael‑Živković, Lidija, Gojgić-Cvijović, Gordana, Karadžić, Ivanka, "Isolation and partial characterization of protease from Pseudomonas aeruginosa ATCC 27853" in Journal of the Serbian Chemical Society, 75, no. 8 (2010):1041-1052,
https://doi.org/10.2298/JSC100125088I . .

TY  - JOUR
AU  - Milić, Jelena
AU  - Beškoski, Vladimir
AU  - Ilić, Mila
AU  - Ali, Samira A. M.
AU  - Gojgić-Cvijović, Gordana
AU  - Vrvić, Miroslav
PY  - 2009
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/530
AB  - Bioremediation, a process that utilizes the capability of microorganism to degrade toxic waste, is emerging as a promising technology for the treatment of soil and groundwater contamination. The technology is very effective in dealing with petroleum hydrocarbon contamination. The aim of this study was to examine the composition of the microbial consortium during the ex situ experiment of bioremediation of soil heavily contaminated with crude oil and its products from the Oil Refinery Pančevo, Serbia. After a 5.5-month experiment with biostimulation and bioventilation, the concentration of the total petroleum hydrocarbons (TPH) had been reduced from 29.80 to 3.29 g/kg (89 %). In soil, the dominant microorganism population comprised Gram-positive bacteria from actinomycete-Nocardia group. The microorganisms which decompose hydrocarbons were the dominant microbial population at the end of the process, with a share of more than 80 % (range 107 CFU/g). On the basis of the results, it was concluded that a stable microbial community had been formed after initial fluctuations.
AB  - Bioremedijacija, proces koji koristi sposobnost mikroorganizama da razlažu toksični otpad, predstavlja savremeni trend u prečišćavanju zagađenog zemljišta i podzemnih voda. Ova tehnologija je veoma efikasna u uklanjanju kontaminacija naftnog zagađivača. Cilj ovog rada je bio karakterizacija konzorcijuma mikroorganizama pri ex citu bioremedijaciji zemljišta teško kontaminiranog naftom i njenim derivatima iz Rafinerije nafte, Pančevo. Posle 5,5 meseci eksperimenta uz biostimulaciju i bioventilaciju koncentracija ukupnih ugljovodonika nafte je smanjena sa 29,80 na 3,29 g/kg (89 %). Dominantna populacija u zemljištu uključuje Gram-pozitivne bakterije iz grupe aktinomiceta-Nocardia. Mikroorganizmi koji razgrađuju ugljovodonike na kraju procesa su bili dominantna mikrobna populacija sa udelom preko 80 % (red veličine 107 CFU/g). Na osnovu ovih rezultata se može zaključiti da je, nakon početnih promena, došlo do formiranja stabilne mikrobne zajednice.
PB  - Serbian Chemical Soc, Belgrade
T2  - Journal of the Serbian Chemical Society
T1  - Bioremediation of soil heavily contaminated with crude oil and its products: Composition of the microbial consortium
T1  - Bioremedijacija zemljišta teško kontaminiranog naftom i naftnim derivatima - sastav konzorcijuma mikroorganizama
VL  - 74
IS  - 4
SP  - 455
EP  - 460
DO  - 10.2298/JSC0904455M
ER  - 

@article{
author = "Milić, Jelena and Beškoski, Vladimir and Ilić, Mila and Ali, Samira A. M. and Gojgić-Cvijović, Gordana and Vrvić, Miroslav",
year = "2009",
abstract = "Bioremediation, a process that utilizes the capability of microorganism to degrade toxic waste, is emerging as a promising technology for the treatment of soil and groundwater contamination. The technology is very effective in dealing with petroleum hydrocarbon contamination. The aim of this study was to examine the composition of the microbial consortium during the ex situ experiment of bioremediation of soil heavily contaminated with crude oil and its products from the Oil Refinery Pančevo, Serbia. After a 5.5-month experiment with biostimulation and bioventilation, the concentration of the total petroleum hydrocarbons (TPH) had been reduced from 29.80 to 3.29 g/kg (89 %). In soil, the dominant microorganism population comprised Gram-positive bacteria from actinomycete-Nocardia group. The microorganisms which decompose hydrocarbons were the dominant microbial population at the end of the process, with a share of more than 80 % (range 107 CFU/g). On the basis of the results, it was concluded that a stable microbial community had been formed after initial fluctuations., Bioremedijacija, proces koji koristi sposobnost mikroorganizama da razlažu toksični otpad, predstavlja savremeni trend u prečišćavanju zagađenog zemljišta i podzemnih voda. Ova tehnologija je veoma efikasna u uklanjanju kontaminacija naftnog zagađivača. Cilj ovog rada je bio karakterizacija konzorcijuma mikroorganizama pri ex citu bioremedijaciji zemljišta teško kontaminiranog naftom i njenim derivatima iz Rafinerije nafte, Pančevo. Posle 5,5 meseci eksperimenta uz biostimulaciju i bioventilaciju koncentracija ukupnih ugljovodonika nafte je smanjena sa 29,80 na 3,29 g/kg (89 %). Dominantna populacija u zemljištu uključuje Gram-pozitivne bakterije iz grupe aktinomiceta-Nocardia. Mikroorganizmi koji razgrađuju ugljovodonike na kraju procesa su bili dominantna mikrobna populacija sa udelom preko 80 % (red veličine 107 CFU/g). Na osnovu ovih rezultata se može zaključiti da je, nakon početnih promena, došlo do formiranja stabilne mikrobne zajednice.",
publisher = "Serbian Chemical Soc, Belgrade",
journal = "Journal of the Serbian Chemical Society",
title = "Bioremediation of soil heavily contaminated with crude oil and its products: Composition of the microbial consortium, Bioremedijacija zemljišta teško kontaminiranog naftom i naftnim derivatima - sastav konzorcijuma mikroorganizama",
volume = "74",
number = "4",
pages = "455-460",
doi = "10.2298/JSC0904455M"
}

Milić, J., Beškoski, V., Ilić, M., Ali, S. A. M., Gojgić-Cvijović, G.,& Vrvić, M.. (2009). Bioremediation of soil heavily contaminated with crude oil and its products: Composition of the microbial consortium. in Journal of the Serbian Chemical Society
Serbian Chemical Soc, Belgrade., 74(4), 455-460.
https://doi.org/10.2298/JSC0904455M

Milić J, Beškoski V, Ilić M, Ali SAM, Gojgić-Cvijović G, Vrvić M. Bioremediation of soil heavily contaminated with crude oil and its products: Composition of the microbial consortium. in Journal of the Serbian Chemical Society. 2009;74(4):455-460.
doi:10.2298/JSC0904455M .

Milić, Jelena, Beškoski, Vladimir, Ilić, Mila, Ali, Samira A. M., Gojgić-Cvijović, Gordana, Vrvić, Miroslav, "Bioremediation of soil heavily contaminated with crude oil and its products: Composition of the microbial consortium" in Journal of the Serbian Chemical Society, 74, no. 4 (2009):455-460,
https://doi.org/10.2298/JSC0904455M . .

TY  - JOUR
AU  - Izrael‑Živković, Lidija
AU  - Gojgić-Cvijović, Gordana
AU  - Gopcevic, K.R.
AU  - Vrvić, Miroslav
AU  - Karadžić, Ivanka
PY  - 2009
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/587
AB  - An extracellular lipase from Pseudomonas aeruginosa ATCC 27853 has been purified and its enzymatic characteristics were determined. According to SDS-PAGE and gel filtration molecular mass estimated to be 30 kDa, what classified the lipase in group I.1. Although 14 lipases from P. aeruginosa with similar molecular mass are referred to date, their basic enzymatic properties have not been reported yet. To address the gap we found: the optimal temperature and pH in water solution being 50 °C and 9.3, respectively; the lipase was inhibited with Hg2+ ions and sodium dodecylsulphate (SDS), while non-ionic detergent Triton X-100 activated the enzyme; the lipase hydrolyzed more rapidly middle chain triglycerides and it was not regiospecific; the lipase demonstrated naturally occurring stability in different organic solvents with concentrations ranging from 30 to 70%, including good thermal stability in 30% organic solvent solution. Even though strain P. aeruginosa ATCC 27853 was not isolated from extreme environment it showed activity in organic solvent suggesting that this lipase is suitable for variety of applications, including reactions in water restricted medium and bioremediation of contaminations by organic solvents.
T2  - Journal of Basic Microbiology
T1  - Enzymatic characterization of 30 kDa lipase from Pseudomonas aeruginosa ATCC 27853
VL  - 49
IS  - 5
SP  - 452
EP  - 462
DO  - 10.1002/jobm.200800229
ER  - 

@article{
author = "Izrael‑Živković, Lidija and Gojgić-Cvijović, Gordana and Gopcevic, K.R. and Vrvić, Miroslav and Karadžić, Ivanka",
year = "2009",
abstract = "An extracellular lipase from Pseudomonas aeruginosa ATCC 27853 has been purified and its enzymatic characteristics were determined. According to SDS-PAGE and gel filtration molecular mass estimated to be 30 kDa, what classified the lipase in group I.1. Although 14 lipases from P. aeruginosa with similar molecular mass are referred to date, their basic enzymatic properties have not been reported yet. To address the gap we found: the optimal temperature and pH in water solution being 50 °C and 9.3, respectively; the lipase was inhibited with Hg2+ ions and sodium dodecylsulphate (SDS), while non-ionic detergent Triton X-100 activated the enzyme; the lipase hydrolyzed more rapidly middle chain triglycerides and it was not regiospecific; the lipase demonstrated naturally occurring stability in different organic solvents with concentrations ranging from 30 to 70%, including good thermal stability in 30% organic solvent solution. Even though strain P. aeruginosa ATCC 27853 was not isolated from extreme environment it showed activity in organic solvent suggesting that this lipase is suitable for variety of applications, including reactions in water restricted medium and bioremediation of contaminations by organic solvents.",
journal = "Journal of Basic Microbiology",
title = "Enzymatic characterization of 30 kDa lipase from Pseudomonas aeruginosa ATCC 27853",
volume = "49",
number = "5",
pages = "452-462",
doi = "10.1002/jobm.200800229"
}

Izrael‑Živković, L., Gojgić-Cvijović, G., Gopcevic, K.R., Vrvić, M.,& Karadžić, I.. (2009). Enzymatic characterization of 30 kDa lipase from Pseudomonas aeruginosa ATCC 27853. in Journal of Basic Microbiology, 49(5), 452-462.
https://doi.org/10.1002/jobm.200800229

Izrael‑Živković L, Gojgić-Cvijović G, Gopcevic K, Vrvić M, Karadžić I. Enzymatic characterization of 30 kDa lipase from Pseudomonas aeruginosa ATCC 27853. in Journal of Basic Microbiology. 2009;49(5):452-462.
doi:10.1002/jobm.200800229 .

Izrael‑Živković, Lidija, Gojgić-Cvijović, Gordana, Gopcevic, K.R., Vrvić, Miroslav, Karadžić, Ivanka, "Enzymatic characterization of 30 kDa lipase from Pseudomonas aeruginosa ATCC 27853" in Journal of Basic Microbiology, 49, no. 5 (2009):452-462,
https://doi.org/10.1002/jobm.200800229 . .

TY  - JOUR
AU  - Bekoski, Vladimir P.
AU  - Milić, Jelena
AU  - Mandić, Boris
AU  - Takic, Milog
AU  - Vrvić, Miroslav
PY  - 2008
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/395
AB  - Oil shale is one of the alternative sources of hydrocarbon fuels ("synthetic petroleum") but is characterized by increased sulfur and nitrogen contents, which represent even greater ecological problems in its use compared to classical fuels. Acidithiobacillus ferrooxidans (At. f.) is capable of oxidizing pyrite to iron(III)-ions, providing a strong oxidation agent at low pH. We have used this oxidizing agent for the oxidation of the sulfur present in dibenzothiophene (DBT), as a substrate model to demonstrate its potential to oxidize organically bound sulfur in oil shale. An HCl-concentrate of oil shale was used as the hydrocarbon matrix. Acidithiobacillus ferrooxidans has already been shown to oxidize the pyritic sulfur component, thereby potentially providing a complete sulfur removal system. By applying GC-MS, we established that DBT transformation occurred by oxidation or elimination of sulfur. The products obtained were more soluble in water than the parent compounds, which reduced the concentration of organic sulfur.
PB  - Elsevier
T2  - Hydrometallurgy
T1  - Removal of organically bound sulfur from oil shale by iron(III)-ion generated-regenerated from pyrite by the action of Acidithiobacillus ferrooxidans - Research on a model system
VL  - 94
IS  - 1-4
SP  - 8
EP  - 13
DO  - 10.1016/j.hydromet.2008.05.015
ER  - 

@article{
author = "Bekoski, Vladimir P. and Milić, Jelena and Mandić, Boris and Takic, Milog and Vrvić, Miroslav",
year = "2008",
abstract = "Oil shale is one of the alternative sources of hydrocarbon fuels ("synthetic petroleum") but is characterized by increased sulfur and nitrogen contents, which represent even greater ecological problems in its use compared to classical fuels. Acidithiobacillus ferrooxidans (At. f.) is capable of oxidizing pyrite to iron(III)-ions, providing a strong oxidation agent at low pH. We have used this oxidizing agent for the oxidation of the sulfur present in dibenzothiophene (DBT), as a substrate model to demonstrate its potential to oxidize organically bound sulfur in oil shale. An HCl-concentrate of oil shale was used as the hydrocarbon matrix. Acidithiobacillus ferrooxidans has already been shown to oxidize the pyritic sulfur component, thereby potentially providing a complete sulfur removal system. By applying GC-MS, we established that DBT transformation occurred by oxidation or elimination of sulfur. The products obtained were more soluble in water than the parent compounds, which reduced the concentration of organic sulfur.",
publisher = "Elsevier",
journal = "Hydrometallurgy",
title = "Removal of organically bound sulfur from oil shale by iron(III)-ion generated-regenerated from pyrite by the action of Acidithiobacillus ferrooxidans - Research on a model system",
volume = "94",
number = "1-4",
pages = "8-13",
doi = "10.1016/j.hydromet.2008.05.015"
}

Bekoski, V. P., Milić, J., Mandić, B., Takic, M.,& Vrvić, M.. (2008). Removal of organically bound sulfur from oil shale by iron(III)-ion generated-regenerated from pyrite by the action of Acidithiobacillus ferrooxidans - Research on a model system. in Hydrometallurgy
Elsevier., 94(1-4), 8-13.
https://doi.org/10.1016/j.hydromet.2008.05.015

Bekoski VP, Milić J, Mandić B, Takic M, Vrvić M. Removal of organically bound sulfur from oil shale by iron(III)-ion generated-regenerated from pyrite by the action of Acidithiobacillus ferrooxidans - Research on a model system. in Hydrometallurgy. 2008;94(1-4):8-13.
doi:10.1016/j.hydromet.2008.05.015 .

Bekoski, Vladimir P., Milić, Jelena, Mandić, Boris, Takic, Milog, Vrvić, Miroslav, "Removal of organically bound sulfur from oil shale by iron(III)-ion generated-regenerated from pyrite by the action of Acidithiobacillus ferrooxidans - Research on a model system" in Hydrometallurgy, 94, no. 1-4 (2008):8-13,
https://doi.org/10.1016/j.hydromet.2008.05.015 . .

TY  - JOUR
AU  - Beškoski, Vladimir
AU  - Matić, Valerija
AU  - Milić, Jelena
AU  - Gođevac, Dejan
AU  - Mandić, Boris
AU  - Vrvić, Miroslav
PY  - 2007
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/312
AB  - Within this paper a new idea for the removal of organically bonded sulphur from fossil fuels is discussed. Dibenzothiophene (DBT) was used as a model compound of organicmolecules containing sulphur. This form of (bio)desulphurization was performed by an indirect mechanism in which iron(III) ions generated from pyrite by Acidithiobacillus ferrooxidans performed the abiotic oxidation. The obtained reaction products, dibenzothiopene sulfoxide and dibenzothiophene sulfone, are more soluble in water than the basic substrate and the obtained results confirmed the basic hypothesis and give the possibility of continuing the experiments related to application of this (bio)desulphurization process. .
AB  - Osnova ovog rada je nova ideja za izdvajanje organski vezanog sumpora iz fosilnih goriva. Dibenzotiofen je upotrebljen kao model-jedinjenje organskih molekula sa sumporom koji su prisutni u fosilnim gorivima. Ovaj novi proces (bio)desulfurizacije se ostvaruje indirektnim mehanizmom, gde se dejstvom Acidithiobacillus ferrooxidans iz pirita generišu gvožđe(III)-joni, koji dalje abiotički oksiduju organski vezani sumpor. Dobijeni reakcioni proizvodi dibenzotiofen-sulfoksid i dibenzotiofen-sulfon su višestruko rastvorljiviji u vodi u odnosu na početno jedinjenje. Dobijeni rezultati potvrđuju osnovnu hipotezu i daju mogućnost nastavka eksperimenata za primenu ovog (bio)desulfurizacionog procesa. .
PB  - Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - Oxidation of dibenzothiophene as a model substrate for the removal of organic sulphur from fossil fuels by iron(III) ions generated from pyrite by Acidithiobacillus ferrooxidans
T1  - Oksidacija dibenzotiofena kao model-supstrata za izdvajanje organskog sumpora iz fosilnih goriva dejstvom gvožđe(III)-jona generisanog iz pirita pomoću Acidithiobacillus ferrooxidans
VL  - 72
IS  - 6
SP  - 533
EP  - 537
DO  - 10.2298/JSC0706533B
ER  - 

@article{
author = "Beškoski, Vladimir and Matić, Valerija and Milić, Jelena and Gođevac, Dejan and Mandić, Boris and Vrvić, Miroslav",
year = "2007",
abstract = "Within this paper a new idea for the removal of organically bonded sulphur from fossil fuels is discussed. Dibenzothiophene (DBT) was used as a model compound of organicmolecules containing sulphur. This form of (bio)desulphurization was performed by an indirect mechanism in which iron(III) ions generated from pyrite by Acidithiobacillus ferrooxidans performed the abiotic oxidation. The obtained reaction products, dibenzothiopene sulfoxide and dibenzothiophene sulfone, are more soluble in water than the basic substrate and the obtained results confirmed the basic hypothesis and give the possibility of continuing the experiments related to application of this (bio)desulphurization process. ., Osnova ovog rada je nova ideja za izdvajanje organski vezanog sumpora iz fosilnih goriva. Dibenzotiofen je upotrebljen kao model-jedinjenje organskih molekula sa sumporom koji su prisutni u fosilnim gorivima. Ovaj novi proces (bio)desulfurizacije se ostvaruje indirektnim mehanizmom, gde se dejstvom Acidithiobacillus ferrooxidans iz pirita generišu gvožđe(III)-joni, koji dalje abiotički oksiduju organski vezani sumpor. Dobijeni reakcioni proizvodi dibenzotiofen-sulfoksid i dibenzotiofen-sulfon su višestruko rastvorljiviji u vodi u odnosu na početno jedinjenje. Dobijeni rezultati potvrđuju osnovnu hipotezu i daju mogućnost nastavka eksperimenata za primenu ovog (bio)desulfurizacionog procesa. .",
publisher = "Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Oxidation of dibenzothiophene as a model substrate for the removal of organic sulphur from fossil fuels by iron(III) ions generated from pyrite by Acidithiobacillus ferrooxidans, Oksidacija dibenzotiofena kao model-supstrata za izdvajanje organskog sumpora iz fosilnih goriva dejstvom gvožđe(III)-jona generisanog iz pirita pomoću Acidithiobacillus ferrooxidans",
volume = "72",
number = "6",
pages = "533-537",
doi = "10.2298/JSC0706533B"
}

Beškoski, V., Matić, V., Milić, J., Gođevac, D., Mandić, B.,& Vrvić, M.. (2007). Oxidation of dibenzothiophene as a model substrate for the removal of organic sulphur from fossil fuels by iron(III) ions generated from pyrite by Acidithiobacillus ferrooxidans. in Journal of the Serbian Chemical Society
Serbian Chemical Society., 72(6), 533-537.
https://doi.org/10.2298/JSC0706533B

Beškoski V, Matić V, Milić J, Gođevac D, Mandić B, Vrvić M. Oxidation of dibenzothiophene as a model substrate for the removal of organic sulphur from fossil fuels by iron(III) ions generated from pyrite by Acidithiobacillus ferrooxidans. in Journal of the Serbian Chemical Society. 2007;72(6):533-537.
doi:10.2298/JSC0706533B .

Beškoski, Vladimir, Matić, Valerija, Milić, Jelena, Gođevac, Dejan, Mandić, Boris, Vrvić, Miroslav, "Oxidation of dibenzothiophene as a model substrate for the removal of organic sulphur from fossil fuels by iron(III) ions generated from pyrite by Acidithiobacillus ferrooxidans" in Journal of the Serbian Chemical Society, 72, no. 6 (2007):533-537,
https://doi.org/10.2298/JSC0706533B . .

TY  - CONF
AU  - Beškoski, Vladimir
AU  - Milić, Jelena
AU  - Mandić, Boris
AU  - Takic, Milos
AU  - Vrvić, Miroslav
PY  - 2007
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/337
AB  - Oil shales are one of the alternative sources of hydrocarbon fuels ("synthetic petroleum"), characterized by the increased sulfur and nitrogen content which represent even greater ecological problem in use, compared to classical fuels. Acidithiobacillus ferrooxidans is capable of oxidizing pyrite to iron (III)-ion, providing a strong oxidation agent at low pH. We have used this oxidizing agent for oxidation of sulfur present in DBT as a substrate model to demonstrate its potential to oxidize organically bound sulfur in oil shales. An HCl-concentrate was used as the hydrocarbon matrix. Acidithiobacillus ferrooxidans is already recognized to oxidize the pyritic sulfur component, thereby potentially providing a complete sulfur removal system. By applying GC-MS we established that DBT transformation occurred by oxidation or elimination of sulfur. The products obtained are more soluble in water than parent compounds and this reduces concentration of organic sulfur.
PB  - Trans Tech Publications Ltd, Durnten-Zurich
C3  - Biohydrometallury: From the Single Cell To the Environment
T1  - Removal of organically bound sulfur from oil shale by iron(III)-ion generated-regenerated from pyrite by the action of Acidithiobacillus ferrooxidans
SP  - 46
DO  - 10.4028/www.scientific.net/AMR.20-21.46
ER  - 

@conference{
author = "Beškoski, Vladimir and Milić, Jelena and Mandić, Boris and Takic, Milos and Vrvić, Miroslav",
year = "2007",
abstract = "Oil shales are one of the alternative sources of hydrocarbon fuels ("synthetic petroleum"), characterized by the increased sulfur and nitrogen content which represent even greater ecological problem in use, compared to classical fuels. Acidithiobacillus ferrooxidans is capable of oxidizing pyrite to iron (III)-ion, providing a strong oxidation agent at low pH. We have used this oxidizing agent for oxidation of sulfur present in DBT as a substrate model to demonstrate its potential to oxidize organically bound sulfur in oil shales. An HCl-concentrate was used as the hydrocarbon matrix. Acidithiobacillus ferrooxidans is already recognized to oxidize the pyritic sulfur component, thereby potentially providing a complete sulfur removal system. By applying GC-MS we established that DBT transformation occurred by oxidation or elimination of sulfur. The products obtained are more soluble in water than parent compounds and this reduces concentration of organic sulfur.",
publisher = "Trans Tech Publications Ltd, Durnten-Zurich",
journal = "Biohydrometallury: From the Single Cell To the Environment",
title = "Removal of organically bound sulfur from oil shale by iron(III)-ion generated-regenerated from pyrite by the action of Acidithiobacillus ferrooxidans",
pages = "46",
doi = "10.4028/www.scientific.net/AMR.20-21.46"
}

Beškoski, V., Milić, J., Mandić, B., Takic, M.,& Vrvić, M.. (2007). Removal of organically bound sulfur from oil shale by iron(III)-ion generated-regenerated from pyrite by the action of Acidithiobacillus ferrooxidans. in Biohydrometallury: From the Single Cell To the Environment
Trans Tech Publications Ltd, Durnten-Zurich., 46.
https://doi.org/10.4028/www.scientific.net/AMR.20-21.46

Beškoski V, Milić J, Mandić B, Takic M, Vrvić M. Removal of organically bound sulfur from oil shale by iron(III)-ion generated-regenerated from pyrite by the action of Acidithiobacillus ferrooxidans. in Biohydrometallury: From the Single Cell To the Environment. 2007;:46.
doi:10.4028/www.scientific.net/AMR.20-21.46 .

Beškoski, Vladimir, Milić, Jelena, Mandić, Boris, Takic, Milos, Vrvić, Miroslav, "Removal of organically bound sulfur from oil shale by iron(III)-ion generated-regenerated from pyrite by the action of Acidithiobacillus ferrooxidans" in Biohydrometallury: From the Single Cell To the Environment (2007):46,
https://doi.org/10.4028/www.scientific.net/AMR.20-21.46 . .