TY  - JOUR
AU  - Spasić, Snežana
AU  - Nikolić-Kokić, Aleksandra
AU  - Miletić, Srđan
AU  - Oreščanin-Dušić, Zorana
AU  - Spasić, Mihajlo
AU  - Blagojević, Duško
AU  - Stević, Zorica
PY  - 2020
UR  - https://www.eurekaselect.com/179582/article
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3966
AB  - Radicava™ (Edaravone) was approved the Food and Drug Administration (FDA) as a new treatment for amyotrophic lateral sclerosis (ALS). Edaravone is a synthetic antioxidant that specifically targets oxidative damage interacting with lipid radicals in the cell. In ALS disease the multiple cell types are involved in devastating loss of motor neurons. Mutations and biochemical changes in various cell types jointly contribute to motor neuron death, disease onset, and disease progression. The overall mechanism of neurodegeneration in ALS is still not completely understood. Dying motor neurons have been reported to exhibit features of apoptosis. However, non-apoptotic features of dying motor neurons have also been reported such as ferroptosis. The role of Edaravone in the prevention of ferroptosis in parallel with other therapeutic approaches to ALS therapy is discussed.
PB  - Bentham Science
T2  - Current Drug Targets
T1  - Edaravone May Prevent Ferroptosis in ALS
VL  - 21
IS  - 8
SP  - 776
EP  - 780
DO  - 10.2174/1389450121666200220123305
ER  - 

@article{
author = "Spasić, Snežana and Nikolić-Kokić, Aleksandra and Miletić, Srđan and Oreščanin-Dušić, Zorana and Spasić, Mihajlo and Blagojević, Duško and Stević, Zorica",
year = "2020",
abstract = "Radicava™ (Edaravone) was approved the Food and Drug Administration (FDA) as a new treatment for amyotrophic lateral sclerosis (ALS). Edaravone is a synthetic antioxidant that specifically targets oxidative damage interacting with lipid radicals in the cell. In ALS disease the multiple cell types are involved in devastating loss of motor neurons. Mutations and biochemical changes in various cell types jointly contribute to motor neuron death, disease onset, and disease progression. The overall mechanism of neurodegeneration in ALS is still not completely understood. Dying motor neurons have been reported to exhibit features of apoptosis. However, non-apoptotic features of dying motor neurons have also been reported such as ferroptosis. The role of Edaravone in the prevention of ferroptosis in parallel with other therapeutic approaches to ALS therapy is discussed.",
publisher = "Bentham Science",
journal = "Current Drug Targets",
title = "Edaravone May Prevent Ferroptosis in ALS",
volume = "21",
number = "8",
pages = "776-780",
doi = "10.2174/1389450121666200220123305"
}

Spasić, S., Nikolić-Kokić, A., Miletić, S., Oreščanin-Dušić, Z., Spasić, M., Blagojević, D.,& Stević, Z.. (2020). Edaravone May Prevent Ferroptosis in ALS. in Current Drug Targets
Bentham Science., 21(8), 776-780.
https://doi.org/10.2174/1389450121666200220123305

Spasić S, Nikolić-Kokić A, Miletić S, Oreščanin-Dušić Z, Spasić M, Blagojević D, Stević Z. Edaravone May Prevent Ferroptosis in ALS. in Current Drug Targets. 2020;21(8):776-780.
doi:10.2174/1389450121666200220123305 .

Spasić, Snežana, Nikolić-Kokić, Aleksandra, Miletić, Srđan, Oreščanin-Dušić, Zorana, Spasić, Mihajlo, Blagojević, Duško, Stević, Zorica, "Edaravone May Prevent Ferroptosis in ALS" in Current Drug Targets, 21, no. 8 (2020):776-780,
https://doi.org/10.2174/1389450121666200220123305 . .

TY  - JOUR
AU  - Uzelac, Tamara N.
AU  - Nikolić-Kokić, Aleksandra
AU  - Spasić, Snežana
AU  - Mačvanin, Mirjana T.
AU  - Nikolić, Milan
AU  - Mandić, Ljuba M.
AU  - Jovanović, Vesna B.
PY  - 2019
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3432
AB  - Antipsychotic drugs interfere with the antioxidant defense system provoking complex and often toxicological effects. Here we examined differences in plasma albumin reduced free thiol (SH) group content and its reactivity as a consequence of clozapine (CLZ) and ziprasidone (ZIP) binding. Chronic administration of CLZ reduced, whereas treatment with ZIP increased albumin-SH content in rats. Regardless of the ratio of stearic acid (SA) bound to protein, in vitro binding of ZIP to human serum albumin (HSA) increased both the SH group level and reactivity. In contrast, the effect of CLZ on HSA-SH reactivity was dependent on HSA to SA molar ratio. CLZ binding was accompanied by an increase in HSA-SH reactivity in samples with normal, but a reduction of itsreactivity level with higher SA/HSA ratio, compared to drug-free samples. We demonstrate by steady-state fluorescence quenching studies that an increase in SA binding to HSA is associated with a significant reduction of binding constant for both antipsychotics. In addition, this is the first report of quantitative characterization of ZIP binding to HSA. Our findings suggest that albumin-SH content and reactivity is modulated by ZIP towards an increased antioxidant defense capacity in circulation, as opposed to CLZ, which can contribute to the safer, more effective treatment of schizophrenia.
PB  - Elsevier
T2  - Chemico-Biological Interactions
T1  - Opposite clozapine and ziprasidone effects on the reactivity of plasma albumin SH-group are the consequence of their different binding properties dependent on protein fatty acids content
VL  - 311
SP  - 108787
DO  - 10.1016/j.cbi.2019.108787
ER  - 

@article{
author = "Uzelac, Tamara N. and Nikolić-Kokić, Aleksandra and Spasić, Snežana and Mačvanin, Mirjana T. and Nikolić, Milan and Mandić, Ljuba M. and Jovanović, Vesna B.",
year = "2019",
abstract = "Antipsychotic drugs interfere with the antioxidant defense system provoking complex and often toxicological effects. Here we examined differences in plasma albumin reduced free thiol (SH) group content and its reactivity as a consequence of clozapine (CLZ) and ziprasidone (ZIP) binding. Chronic administration of CLZ reduced, whereas treatment with ZIP increased albumin-SH content in rats. Regardless of the ratio of stearic acid (SA) bound to protein, in vitro binding of ZIP to human serum albumin (HSA) increased both the SH group level and reactivity. In contrast, the effect of CLZ on HSA-SH reactivity was dependent on HSA to SA molar ratio. CLZ binding was accompanied by an increase in HSA-SH reactivity in samples with normal, but a reduction of itsreactivity level with higher SA/HSA ratio, compared to drug-free samples. We demonstrate by steady-state fluorescence quenching studies that an increase in SA binding to HSA is associated with a significant reduction of binding constant for both antipsychotics. In addition, this is the first report of quantitative characterization of ZIP binding to HSA. Our findings suggest that albumin-SH content and reactivity is modulated by ZIP towards an increased antioxidant defense capacity in circulation, as opposed to CLZ, which can contribute to the safer, more effective treatment of schizophrenia.",
publisher = "Elsevier",
journal = "Chemico-Biological Interactions",
title = "Opposite clozapine and ziprasidone effects on the reactivity of plasma albumin SH-group are the consequence of their different binding properties dependent on protein fatty acids content",
volume = "311",
pages = "108787",
doi = "10.1016/j.cbi.2019.108787"
}

Uzelac, T. N., Nikolić-Kokić, A., Spasić, S., Mačvanin, M. T., Nikolić, M., Mandić, L. M.,& Jovanović, V. B.. (2019). Opposite clozapine and ziprasidone effects on the reactivity of plasma albumin SH-group are the consequence of their different binding properties dependent on protein fatty acids content. in Chemico-Biological Interactions
Elsevier., 311, 108787.
https://doi.org/10.1016/j.cbi.2019.108787

Uzelac TN, Nikolić-Kokić A, Spasić S, Mačvanin MT, Nikolić M, Mandić LM, Jovanović VB. Opposite clozapine and ziprasidone effects on the reactivity of plasma albumin SH-group are the consequence of their different binding properties dependent on protein fatty acids content. in Chemico-Biological Interactions. 2019;311:108787.
doi:10.1016/j.cbi.2019.108787 .

Uzelac, Tamara N., Nikolić-Kokić, Aleksandra, Spasić, Snežana, Mačvanin, Mirjana T., Nikolić, Milan, Mandić, Ljuba M., Jovanović, Vesna B., "Opposite clozapine and ziprasidone effects on the reactivity of plasma albumin SH-group are the consequence of their different binding properties dependent on protein fatty acids content" in Chemico-Biological Interactions, 311 (2019):108787,
https://doi.org/10.1016/j.cbi.2019.108787 . .

TY  - JOUR
AU  - Uzelac, Tamara N.
AU  - Nikolić-Kokić, Aleksandra
AU  - Spasić, Snežana
AU  - Mačvanin, Mirjana T.
AU  - Nikolić, Milan
AU  - Mandić, Ljuba M.
AU  - Jovanović, Vesna B.
PY  - 2019
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3053
AB  - Antipsychotic drugs interfere with the antioxidant defense system provoking complex and often toxicological effects. Here we examined differences in plasma albumin reduced free thiol (SH) group content and its reactivity as a consequence of clozapine (CLZ) and ziprasidone (ZIP) binding. Chronic administration of CLZ reduced, whereas treatment with ZIP increased albumin-SH content in rats. Regardless of the ratio of stearic acid (SA) bound to protein, in vitro binding of ZIP to human serum albumin (HSA) increased both the SH group level and reactivity. In contrast, the effect of CLZ on HSA-SH reactivity was dependent on HSA to SA molar ratio. CLZ binding was accompanied by an increase in HSA-SH reactivity in samples with normal, but a reduction of its
reactivity level with higher SA/HSA ratio, compared to drug-free samples. We demonstrate by steady-state fluorescence quenching studies that an increase in SA binding to HSA is associated with a significant reduction of binding constant for both antipsychotics. In addition, this is the first report of quantitative characterization of ZIP binding to HSA. Our findings suggest that albumin-SH content and reactivity is modulated by ZIP towards an increased antioxidant defense capacity in circulation, as opposed to CLZ, which can contribute to the safer, more effective treatment of schizophrenia.
PB  - Elsevier
T2  - Chemico-Biological Interactions
T1  - Opposite clozapine and ziprasidone effects on the reactivity of plasma albumin SH-group are the consequence of their different binding properties dependent on protein fatty acids content
VL  - 311
SP  - 108787
DO  - 10.1016/j.cbi.2019.108787
ER  - 

@article{
author = "Uzelac, Tamara N. and Nikolić-Kokić, Aleksandra and Spasić, Snežana and Mačvanin, Mirjana T. and Nikolić, Milan and Mandić, Ljuba M. and Jovanović, Vesna B.",
year = "2019",
abstract = "Antipsychotic drugs interfere with the antioxidant defense system provoking complex and often toxicological effects. Here we examined differences in plasma albumin reduced free thiol (SH) group content and its reactivity as a consequence of clozapine (CLZ) and ziprasidone (ZIP) binding. Chronic administration of CLZ reduced, whereas treatment with ZIP increased albumin-SH content in rats. Regardless of the ratio of stearic acid (SA) bound to protein, in vitro binding of ZIP to human serum albumin (HSA) increased both the SH group level and reactivity. In contrast, the effect of CLZ on HSA-SH reactivity was dependent on HSA to SA molar ratio. CLZ binding was accompanied by an increase in HSA-SH reactivity in samples with normal, but a reduction of its
reactivity level with higher SA/HSA ratio, compared to drug-free samples. We demonstrate by steady-state fluorescence quenching studies that an increase in SA binding to HSA is associated with a significant reduction of binding constant for both antipsychotics. In addition, this is the first report of quantitative characterization of ZIP binding to HSA. Our findings suggest that albumin-SH content and reactivity is modulated by ZIP towards an increased antioxidant defense capacity in circulation, as opposed to CLZ, which can contribute to the safer, more effective treatment of schizophrenia.",
publisher = "Elsevier",
journal = "Chemico-Biological Interactions",
title = "Opposite clozapine and ziprasidone effects on the reactivity of plasma albumin SH-group are the consequence of their different binding properties dependent on protein fatty acids content",
volume = "311",
pages = "108787",
doi = "10.1016/j.cbi.2019.108787"
}

Uzelac, T. N., Nikolić-Kokić, A., Spasić, S., Mačvanin, M. T., Nikolić, M., Mandić, L. M.,& Jovanović, V. B.. (2019). Opposite clozapine and ziprasidone effects on the reactivity of plasma albumin SH-group are the consequence of their different binding properties dependent on protein fatty acids content. in Chemico-Biological Interactions
Elsevier., 311, 108787.
https://doi.org/10.1016/j.cbi.2019.108787

Uzelac TN, Nikolić-Kokić A, Spasić S, Mačvanin MT, Nikolić M, Mandić LM, Jovanović VB. Opposite clozapine and ziprasidone effects on the reactivity of plasma albumin SH-group are the consequence of their different binding properties dependent on protein fatty acids content. in Chemico-Biological Interactions. 2019;311:108787.
doi:10.1016/j.cbi.2019.108787 .

Uzelac, Tamara N., Nikolić-Kokić, Aleksandra, Spasić, Snežana, Mačvanin, Mirjana T., Nikolić, Milan, Mandić, Ljuba M., Jovanović, Vesna B., "Opposite clozapine and ziprasidone effects on the reactivity of plasma albumin SH-group are the consequence of their different binding properties dependent on protein fatty acids content" in Chemico-Biological Interactions, 311 (2019):108787,
https://doi.org/10.1016/j.cbi.2019.108787 . .

TY  - CONF
AU  - Savić, Danijela
AU  - Opačić, Miloš
AU  - Ristić, Aleksandar J.
AU  - Sokić, Dragoslav
AU  - Baščarević, Vladimir
AU  - Raičević, Savo
AU  - Savić, Slobodan
AU  - Živin, Marko
AU  - Šelih, Vid Simon
AU  - Spasić, Snežana
AU  - Spasojević, Ivan
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3543
AB  - The accumulating evidence on the relation between the disturbed metal homeostasis and epilepsy urges the need for data regarding the total metal concentrations, as well as metal distribution in the brain itself, in order to indicate where to direct the potential therapy, to metal supplementation or chelation. This paper summarizes our results on the measurements of some important essential metals in hippocampi of patients with mesial temporal lobe epilepsy (mTLE) who underwent amigdalohippocampectomy. The key findings point out that levels of copper and manganese are deficient in hippocampi of mTLE patients, and that their concentrations correlated positively with neuronal loss in affected regions of sclerotic hippocampus. In addition, the Cu concentration was decreased in the areas of total neuronal loss. Iron and zinc total hippocampal levels were neither accumulated nor deficient compared to control. Our results contribute to deeper insight of metals
biology in the epilepsy and may represent the initial point of new and non-invasive therapy of drug resistant epilepsy.
PB  - Serbian Biochemical Society
C3  - Serbian Biochemical Society Eighth Conference with international participation, “Coordination in Biochemistry and Life”, University of Novi Sad – Rectorate Hall, 16.11.2018. Novi Sad, Serbia
T1  - Distribution and role of metals in sclerotic hippocampi of patients with mesial temporal lobe epilepsy
SP  - 105
EP  - 112
UR  - https://hdl.handle.net/21.15107/rcub_cer_3543
ER  - 

@conference{
author = "Savić, Danijela and Opačić, Miloš and Ristić, Aleksandar J. and Sokić, Dragoslav and Baščarević, Vladimir and Raičević, Savo and Savić, Slobodan and Živin, Marko and Šelih, Vid Simon and Spasić, Snežana and Spasojević, Ivan",
year = "2018",
abstract = "The accumulating evidence on the relation between the disturbed metal homeostasis and epilepsy urges the need for data regarding the total metal concentrations, as well as metal distribution in the brain itself, in order to indicate where to direct the potential therapy, to metal supplementation or chelation. This paper summarizes our results on the measurements of some important essential metals in hippocampi of patients with mesial temporal lobe epilepsy (mTLE) who underwent amigdalohippocampectomy. The key findings point out that levels of copper and manganese are deficient in hippocampi of mTLE patients, and that their concentrations correlated positively with neuronal loss in affected regions of sclerotic hippocampus. In addition, the Cu concentration was decreased in the areas of total neuronal loss. Iron and zinc total hippocampal levels were neither accumulated nor deficient compared to control. Our results contribute to deeper insight of metals
biology in the epilepsy and may represent the initial point of new and non-invasive therapy of drug resistant epilepsy.",
publisher = "Serbian Biochemical Society",
journal = "Serbian Biochemical Society Eighth Conference with international participation, “Coordination in Biochemistry and Life”, University of Novi Sad – Rectorate Hall, 16.11.2018. Novi Sad, Serbia",
title = "Distribution and role of metals in sclerotic hippocampi of patients with mesial temporal lobe epilepsy",
pages = "105-112",
url = "https://hdl.handle.net/21.15107/rcub_cer_3543"
}

Savić, D., Opačić, M., Ristić, A. J., Sokić, D., Baščarević, V., Raičević, S., Savić, S., Živin, M., Šelih, V. S., Spasić, S.,& Spasojević, I.. (2018). Distribution and role of metals in sclerotic hippocampi of patients with mesial temporal lobe epilepsy. in Serbian Biochemical Society Eighth Conference with international participation, “Coordination in Biochemistry and Life”, University of Novi Sad – Rectorate Hall, 16.11.2018. Novi Sad, Serbia
Serbian Biochemical Society., 105-112.
https://hdl.handle.net/21.15107/rcub_cer_3543

Savić D, Opačić M, Ristić AJ, Sokić D, Baščarević V, Raičević S, Savić S, Živin M, Šelih VS, Spasić S, Spasojević I. Distribution and role of metals in sclerotic hippocampi of patients with mesial temporal lobe epilepsy. in Serbian Biochemical Society Eighth Conference with international participation, “Coordination in Biochemistry and Life”, University of Novi Sad – Rectorate Hall, 16.11.2018. Novi Sad, Serbia. 2018;:105-112.
https://hdl.handle.net/21.15107/rcub_cer_3543 .

Savić, Danijela, Opačić, Miloš, Ristić, Aleksandar J., Sokić, Dragoslav, Baščarević, Vladimir, Raičević, Savo, Savić, Slobodan, Živin, Marko, Šelih, Vid Simon, Spasić, Snežana, Spasojević, Ivan, "Distribution and role of metals in sclerotic hippocampi of patients with mesial temporal lobe epilepsy" in Serbian Biochemical Society Eighth Conference with international participation, “Coordination in Biochemistry and Life”, University of Novi Sad – Rectorate Hall, 16.11.2018. Novi Sad, Serbia (2018):105-112,
https://hdl.handle.net/21.15107/rcub_cer_3543 .

TY  - JOUR
AU  - Božić, Bojana
AU  - Korać, Jelena
AU  - Stanković, Dalibor
AU  - Stanić, Marina
AU  - Romanović, Mima
AU  - Bogdanović Pristov, Jelena
AU  - Spasić, Snežana
AU  - Popović-Bjelić, Ana
AU  - Spasojević, Ivan
AU  - Bajčetić, Milica
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3936
AB  - An increase in the copper pool in body fluids has been related to a number of pathological conditions, including infections. Copper ions may affect antibiotics via the formation of coordination bonds and/or redox reactions. Herein, we analyzed the interactions of Cu2+ with eight β-lactam antibiotics using UV–Vis spectrophotometry, EPR spectroscopy, and electrochemical methods. Penicillin G did not show any detectable interactions with Cu2+. Ampicillin, amoxicillin and cephalexin formed stable colored complexes with octahedral coordination environment of Cu2+ with tetragonal distortion, and primary amine group as the site of coordinate bond formation. These β-lactams increased the solubility of Cu2+ in the phosphate buffer. Ceftazidime and Cu2+ formed a complex with a similar geometry and gave rise to an organic radical. Ceftriaxone-Cu2+ complex appears to exhibit different geometry. All complexes showed 1:1 stoichiometry. Cefaclor reduced Cu2+ to Cu1+ that further reacted with molecular oxygen to produce hydrogen peroxide. Finally, meropenem underwent degradation in the presence of copper. The analysis of activity against Escherichia coli and Staphylococcus aureus showed that the effects of meropenem, amoxicillin, ampicillin, and ceftriaxone were significantly hindered in the presence of copper ions. The interactions with copper ions should be taken into account regarding the problem of antibiotic resistance and in the selection of the most efficient antimicrobial therapy for patients with altered copper homeostasis.
PB  - Elsevier
T2  - Free Radical Biology and Medicine
T1  - Coordination and redox interactions of β-lactam antibiotics with Cu2+ in physiological settings and the impact on antibacterial activity
VL  - 129
SP  - 279
EP  - 285
DO  - 10.1016/j.freeradbiomed.2018.09.038
ER  - 

@article{
author = "Božić, Bojana and Korać, Jelena and Stanković, Dalibor and Stanić, Marina and Romanović, Mima and Bogdanović Pristov, Jelena and Spasić, Snežana and Popović-Bjelić, Ana and Spasojević, Ivan and Bajčetić, Milica",
year = "2018",
abstract = "An increase in the copper pool in body fluids has been related to a number of pathological conditions, including infections. Copper ions may affect antibiotics via the formation of coordination bonds and/or redox reactions. Herein, we analyzed the interactions of Cu2+ with eight β-lactam antibiotics using UV–Vis spectrophotometry, EPR spectroscopy, and electrochemical methods. Penicillin G did not show any detectable interactions with Cu2+. Ampicillin, amoxicillin and cephalexin formed stable colored complexes with octahedral coordination environment of Cu2+ with tetragonal distortion, and primary amine group as the site of coordinate bond formation. These β-lactams increased the solubility of Cu2+ in the phosphate buffer. Ceftazidime and Cu2+ formed a complex with a similar geometry and gave rise to an organic radical. Ceftriaxone-Cu2+ complex appears to exhibit different geometry. All complexes showed 1:1 stoichiometry. Cefaclor reduced Cu2+ to Cu1+ that further reacted with molecular oxygen to produce hydrogen peroxide. Finally, meropenem underwent degradation in the presence of copper. The analysis of activity against Escherichia coli and Staphylococcus aureus showed that the effects of meropenem, amoxicillin, ampicillin, and ceftriaxone were significantly hindered in the presence of copper ions. The interactions with copper ions should be taken into account regarding the problem of antibiotic resistance and in the selection of the most efficient antimicrobial therapy for patients with altered copper homeostasis.",
publisher = "Elsevier",
journal = "Free Radical Biology and Medicine",
title = "Coordination and redox interactions of β-lactam antibiotics with Cu2+ in physiological settings and the impact on antibacterial activity",
volume = "129",
pages = "279-285",
doi = "10.1016/j.freeradbiomed.2018.09.038"
}

Božić, B., Korać, J., Stanković, D., Stanić, M., Romanović, M., Bogdanović Pristov, J., Spasić, S., Popović-Bjelić, A., Spasojević, I.,& Bajčetić, M.. (2018). Coordination and redox interactions of β-lactam antibiotics with Cu2+ in physiological settings and the impact on antibacterial activity. in Free Radical Biology and Medicine
Elsevier., 129, 279-285.
https://doi.org/10.1016/j.freeradbiomed.2018.09.038

Božić B, Korać J, Stanković D, Stanić M, Romanović M, Bogdanović Pristov J, Spasić S, Popović-Bjelić A, Spasojević I, Bajčetić M. Coordination and redox interactions of β-lactam antibiotics with Cu2+ in physiological settings and the impact on antibacterial activity. in Free Radical Biology and Medicine. 2018;129:279-285.
doi:10.1016/j.freeradbiomed.2018.09.038 .

Božić, Bojana, Korać, Jelena, Stanković, Dalibor, Stanić, Marina, Romanović, Mima, Bogdanović Pristov, Jelena, Spasić, Snežana, Popović-Bjelić, Ana, Spasojević, Ivan, Bajčetić, Milica, "Coordination and redox interactions of β-lactam antibiotics with Cu2+ in physiological settings and the impact on antibacterial activity" in Free Radical Biology and Medicine, 129 (2018):279-285,
https://doi.org/10.1016/j.freeradbiomed.2018.09.038 . .

TY  - JOUR
AU  - Božić, Bojana
AU  - Korać, Jelena
AU  - Stanković, Dalibor
AU  - Stanić, Marina
AU  - Romanović, Mima
AU  - Bogdanović Pristov, Jelena
AU  - Spasić, Snežana
AU  - Popović-Bjelić, Ana
AU  - Spasojević, Ivan
AU  - Bajčetić, Milica
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4281
AB  - An increase in the copper pool in body fluids has been related to a number of pathological conditions, including infections. Copper ions may affect antibiotics via the formation of coordination bonds and/or redox reactions. Herein, we analyzed the interactions of Cu2+ with eight β-lactam antibiotics using UV–Vis spectrophotometry, EPR spectroscopy, and electrochemical methods. Penicillin G did not show any detectable interactions with Cu2+. Ampicillin, amoxicillin and cephalexin formed stable colored complexes with octahedral coordination environment of Cu2+ with tetragonal distortion, and primary amine group as the site of coordinate bond formation. These β-lactams increased the solubility of Cu2+ in the phosphate buffer. Ceftazidime and Cu2+ formed a complex with a similar geometry and gave rise to an organic radical. Ceftriaxone-Cu2+ complex appears to exhibit different geometry. All complexes showed 1:1 stoichiometry. Cefaclor reduced Cu2+ to Cu1+ that further reacted with molecular oxygen to produce hydrogen peroxide. Finally, meropenem underwent degradation in the presence of copper. The analysis of activity against Escherichia coli and Staphylococcus aureus showed that the effects of meropenem, amoxicillin, ampicillin, and ceftriaxone were significantly hindered in the presence of copper ions. The interactions with copper ions should be taken into account regarding the problem of antibiotic resistance and in the selection of the most efficient antimicrobial therapy for patients with altered copper homeostasis.
PB  - Elsevier
T2  - Free Radical Biology and Medicine
T1  - Coordination and redox interactions of β-lactam antibiotics with Cu2+ in physiological settings and the impact on antibacterial activity
VL  - 129
SP  - 279
EP  - 285
DO  - 10.1016/j.freeradbiomed.2018.09.038
ER  - 

@article{
author = "Božić, Bojana and Korać, Jelena and Stanković, Dalibor and Stanić, Marina and Romanović, Mima and Bogdanović Pristov, Jelena and Spasić, Snežana and Popović-Bjelić, Ana and Spasojević, Ivan and Bajčetić, Milica",
year = "2018",
abstract = "An increase in the copper pool in body fluids has been related to a number of pathological conditions, including infections. Copper ions may affect antibiotics via the formation of coordination bonds and/or redox reactions. Herein, we analyzed the interactions of Cu2+ with eight β-lactam antibiotics using UV–Vis spectrophotometry, EPR spectroscopy, and electrochemical methods. Penicillin G did not show any detectable interactions with Cu2+. Ampicillin, amoxicillin and cephalexin formed stable colored complexes with octahedral coordination environment of Cu2+ with tetragonal distortion, and primary amine group as the site of coordinate bond formation. These β-lactams increased the solubility of Cu2+ in the phosphate buffer. Ceftazidime and Cu2+ formed a complex with a similar geometry and gave rise to an organic radical. Ceftriaxone-Cu2+ complex appears to exhibit different geometry. All complexes showed 1:1 stoichiometry. Cefaclor reduced Cu2+ to Cu1+ that further reacted with molecular oxygen to produce hydrogen peroxide. Finally, meropenem underwent degradation in the presence of copper. The analysis of activity against Escherichia coli and Staphylococcus aureus showed that the effects of meropenem, amoxicillin, ampicillin, and ceftriaxone were significantly hindered in the presence of copper ions. The interactions with copper ions should be taken into account regarding the problem of antibiotic resistance and in the selection of the most efficient antimicrobial therapy for patients with altered copper homeostasis.",
publisher = "Elsevier",
journal = "Free Radical Biology and Medicine",
title = "Coordination and redox interactions of β-lactam antibiotics with Cu2+ in physiological settings and the impact on antibacterial activity",
volume = "129",
pages = "279-285",
doi = "10.1016/j.freeradbiomed.2018.09.038"
}

Božić, B., Korać, J., Stanković, D., Stanić, M., Romanović, M., Bogdanović Pristov, J., Spasić, S., Popović-Bjelić, A., Spasojević, I.,& Bajčetić, M.. (2018). Coordination and redox interactions of β-lactam antibiotics with Cu2+ in physiological settings and the impact on antibacterial activity. in Free Radical Biology and Medicine
Elsevier., 129, 279-285.
https://doi.org/10.1016/j.freeradbiomed.2018.09.038

Božić B, Korać J, Stanković D, Stanić M, Romanović M, Bogdanović Pristov J, Spasić S, Popović-Bjelić A, Spasojević I, Bajčetić M. Coordination and redox interactions of β-lactam antibiotics with Cu2+ in physiological settings and the impact on antibacterial activity. in Free Radical Biology and Medicine. 2018;129:279-285.
doi:10.1016/j.freeradbiomed.2018.09.038 .

Božić, Bojana, Korać, Jelena, Stanković, Dalibor, Stanić, Marina, Romanović, Mima, Bogdanović Pristov, Jelena, Spasić, Snežana, Popović-Bjelić, Ana, Spasojević, Ivan, Bajčetić, Milica, "Coordination and redox interactions of β-lactam antibiotics with Cu2+ in physiological settings and the impact on antibacterial activity" in Free Radical Biology and Medicine, 129 (2018):279-285,
https://doi.org/10.1016/j.freeradbiomed.2018.09.038 . .

TY  - DATA
AU  - Božić, Bojana
AU  - Korać, Jelena
AU  - Stanković, Dalibor
AU  - Stanić, Marina
AU  - Romanović, Mima
AU  - Bogdanović Pristov, Jelena
AU  - Spasić, Snežana
AU  - Popović-Bjelić, Ana
AU  - Spasojević, Ivan
AU  - Bajčetić, Milica
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4489
AB  - Figure S1. Speciation diagrams of Cu2+ in the phosphate buffer. Diagrams were prepared in Hydra-Medusa Software, using the presented parameters.
PB  - Elsevier
T2  - Free Radical Biology and Medicine
T1  - Supporting Information for: "Coordination and redox interactions of β-lactam antibiotics with Cu2+ in physiological settings and the impact on antibacterial activity"
UR  - https://hdl.handle.net/21.15107/rcub_cer_4489
ER  - 

@misc{
author = "Božić, Bojana and Korać, Jelena and Stanković, Dalibor and Stanić, Marina and Romanović, Mima and Bogdanović Pristov, Jelena and Spasić, Snežana and Popović-Bjelić, Ana and Spasojević, Ivan and Bajčetić, Milica",
year = "2018",
abstract = "Figure S1. Speciation diagrams of Cu2+ in the phosphate buffer. Diagrams were prepared in Hydra-Medusa Software, using the presented parameters.",
publisher = "Elsevier",
journal = "Free Radical Biology and Medicine",
title = "Supporting Information for: "Coordination and redox interactions of β-lactam antibiotics with Cu2+ in physiological settings and the impact on antibacterial activity"",
url = "https://hdl.handle.net/21.15107/rcub_cer_4489"
}

Božić, B., Korać, J., Stanković, D., Stanić, M., Romanović, M., Bogdanović Pristov, J., Spasić, S., Popović-Bjelić, A., Spasojević, I.,& Bajčetić, M.. (2018). Supporting Information for: "Coordination and redox interactions of β-lactam antibiotics with Cu2+ in physiological settings and the impact on antibacterial activity". in Free Radical Biology and Medicine
Elsevier..
https://hdl.handle.net/21.15107/rcub_cer_4489

Božić B, Korać J, Stanković D, Stanić M, Romanović M, Bogdanović Pristov J, Spasić S, Popović-Bjelić A, Spasojević I, Bajčetić M. Supporting Information for: "Coordination and redox interactions of β-lactam antibiotics with Cu2+ in physiological settings and the impact on antibacterial activity". in Free Radical Biology and Medicine. 2018;.
https://hdl.handle.net/21.15107/rcub_cer_4489 .

Božić, Bojana, Korać, Jelena, Stanković, Dalibor, Stanić, Marina, Romanović, Mima, Bogdanović Pristov, Jelena, Spasić, Snežana, Popović-Bjelić, Ana, Spasojević, Ivan, Bajčetić, Milica, "Supporting Information for: "Coordination and redox interactions of β-lactam antibiotics with Cu2+ in physiological settings and the impact on antibacterial activity"" in Free Radical Biology and Medicine (2018),
https://hdl.handle.net/21.15107/rcub_cer_4489 .

TY  - JOUR
AU  - Martinov, Jelena
AU  - Krstic, Miodrag
AU  - Spasić, Snežana
AU  - Miletić, Srđan
AU  - Stefanovic-Kojic, Jovana
AU  - Nikolić-Kokić, Aleksandra
AU  - Blagojevic, Dusko
AU  - Spasojević, Ivan
AU  - Spasić, Mihajlo
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2093
AB  - Pectin is the main soluble fiber in apples or citruses. It may be fermented by gut microbiota to metabolites showing local intestinal and systemic effects. A wide range of beneficial effects of dietary pectin includes impacts on the redox milieu and microbiota profile. We prepared pectin-derived oligosaccharides (apple (APDO) and citrus) and polygalacturonic acid-derived oligosaccharides, using alkaline hydrolysis by hydrogen peroxide, and analyzed them by Foufier Transform Infrared spectrometry. Furthermore, we analyzed the effects of pectin derived oligosaccharides on hydroxyl radical (HO center dot)-generating Fenton reaction using electron paramagnetic resonance spin-trapping spectroscopy, and the effects on the growth of Escherichia coli and Staphylococcus minus in the presence of dietary-relevant HO center dot-generating system (iron + ascorbate). The oligosaccharides react with HO center dot radical to produce carbon dioxide radical anion (CO2 center dot (-)). A comparative analysis showed that APDO has the most prominent bacteriostatic effect. This might be at least partially related to the higher capacity of APDO to produce CO2 center dot (-), which specifically targets proteins and appears to have a longer lifetime and larger diffusion radius in biological systems compared to HO center dot.
PB  - Elsevier
T2  - Food Research International
T1  - Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus
VL  - 100
SP  - 132
EP  - 136
DO  - 10.1016/j.foodres.2017.08.040
ER  - 

@article{
author = "Martinov, Jelena and Krstic, Miodrag and Spasić, Snežana and Miletić, Srđan and Stefanovic-Kojic, Jovana and Nikolić-Kokić, Aleksandra and Blagojevic, Dusko and Spasojević, Ivan and Spasić, Mihajlo",
year = "2017",
abstract = "Pectin is the main soluble fiber in apples or citruses. It may be fermented by gut microbiota to metabolites showing local intestinal and systemic effects. A wide range of beneficial effects of dietary pectin includes impacts on the redox milieu and microbiota profile. We prepared pectin-derived oligosaccharides (apple (APDO) and citrus) and polygalacturonic acid-derived oligosaccharides, using alkaline hydrolysis by hydrogen peroxide, and analyzed them by Foufier Transform Infrared spectrometry. Furthermore, we analyzed the effects of pectin derived oligosaccharides on hydroxyl radical (HO center dot)-generating Fenton reaction using electron paramagnetic resonance spin-trapping spectroscopy, and the effects on the growth of Escherichia coli and Staphylococcus minus in the presence of dietary-relevant HO center dot-generating system (iron + ascorbate). The oligosaccharides react with HO center dot radical to produce carbon dioxide radical anion (CO2 center dot (-)). A comparative analysis showed that APDO has the most prominent bacteriostatic effect. This might be at least partially related to the higher capacity of APDO to produce CO2 center dot (-), which specifically targets proteins and appears to have a longer lifetime and larger diffusion radius in biological systems compared to HO center dot.",
publisher = "Elsevier",
journal = "Food Research International",
title = "Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus",
volume = "100",
pages = "132-136",
doi = "10.1016/j.foodres.2017.08.040"
}

Martinov, J., Krstic, M., Spasić, S., Miletić, S., Stefanovic-Kojic, J., Nikolić-Kokić, A., Blagojevic, D., Spasojević, I.,& Spasić, M.. (2017). Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus. in Food Research International
Elsevier., 100, 132-136.
https://doi.org/10.1016/j.foodres.2017.08.040

Martinov J, Krstic M, Spasić S, Miletić S, Stefanovic-Kojic J, Nikolić-Kokić A, Blagojevic D, Spasojević I, Spasić M. Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus. in Food Research International. 2017;100:132-136.
doi:10.1016/j.foodres.2017.08.040 .

Martinov, Jelena, Krstic, Miodrag, Spasić, Snežana, Miletić, Srđan, Stefanovic-Kojic, Jovana, Nikolić-Kokić, Aleksandra, Blagojevic, Dusko, Spasojević, Ivan, Spasić, Mihajlo, "Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus" in Food Research International, 100 (2017):132-136,
https://doi.org/10.1016/j.foodres.2017.08.040 . .

TY  - JOUR
AU  - Martinov, Jelena
AU  - Krstic, Miodrag
AU  - Spasić, Snežana
AU  - Miletić, Srđan
AU  - Stefanovic-Kojic, Jovana
AU  - Nikolić-Kokić, Aleksandra
AU  - Blagojevic, Dusko
AU  - Spasojević, Ivan
AU  - Spasić, Mihajlo
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3043
AB  - Pectin is the main soluble fiber in apples or citruses. It may be fermented by gut microbiota to metabolites showing local intestinal and systemic effects. A wide range of beneficial effects of dietary pectin includes impacts on the redox milieu and microbiota profile. We prepared pectin-derived oligosaccharides (apple (APDO) and citrus) and polygalacturonic acid-derived oligosaccharides, using alkaline hydrolysis by hydrogen peroxide, and analyzed them by Foufier Transform Infrared spectrometry. Furthermore, we analyzed the effects of pectin derived oligosaccharides on hydroxyl radical (HO center dot)-generating Fenton reaction using electron paramagnetic resonance spin-trapping spectroscopy, and the effects on the growth of Escherichia coli and Staphylococcus minus in the presence of dietary-relevant HO center dot-generating system (iron + ascorbate). The oligosaccharides react with HO center dot radical to produce carbon dioxide radical anion (CO2 center dot (-)). A comparative analysis showed that APDO has the most prominent bacteriostatic effect. This might be at least partially related to the higher capacity of APDO to produce CO2 center dot (-), which specifically targets proteins and appears to have a longer lifetime and larger diffusion radius in biological systems compared to HO center dot.
PB  - Elsevier
T2  - Food Research International
T1  - Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus
VL  - 100
SP  - 132
EP  - 136
DO  - 10.1016/j.foodres.2017.08.040
ER  - 

@article{
author = "Martinov, Jelena and Krstic, Miodrag and Spasić, Snežana and Miletić, Srđan and Stefanovic-Kojic, Jovana and Nikolić-Kokić, Aleksandra and Blagojevic, Dusko and Spasojević, Ivan and Spasić, Mihajlo",
year = "2017",
abstract = "Pectin is the main soluble fiber in apples or citruses. It may be fermented by gut microbiota to metabolites showing local intestinal and systemic effects. A wide range of beneficial effects of dietary pectin includes impacts on the redox milieu and microbiota profile. We prepared pectin-derived oligosaccharides (apple (APDO) and citrus) and polygalacturonic acid-derived oligosaccharides, using alkaline hydrolysis by hydrogen peroxide, and analyzed them by Foufier Transform Infrared spectrometry. Furthermore, we analyzed the effects of pectin derived oligosaccharides on hydroxyl radical (HO center dot)-generating Fenton reaction using electron paramagnetic resonance spin-trapping spectroscopy, and the effects on the growth of Escherichia coli and Staphylococcus minus in the presence of dietary-relevant HO center dot-generating system (iron + ascorbate). The oligosaccharides react with HO center dot radical to produce carbon dioxide radical anion (CO2 center dot (-)). A comparative analysis showed that APDO has the most prominent bacteriostatic effect. This might be at least partially related to the higher capacity of APDO to produce CO2 center dot (-), which specifically targets proteins and appears to have a longer lifetime and larger diffusion radius in biological systems compared to HO center dot.",
publisher = "Elsevier",
journal = "Food Research International",
title = "Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus",
volume = "100",
pages = "132-136",
doi = "10.1016/j.foodres.2017.08.040"
}

Martinov, J., Krstic, M., Spasić, S., Miletić, S., Stefanovic-Kojic, J., Nikolić-Kokić, A., Blagojevic, D., Spasojević, I.,& Spasić, M.. (2017). Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus. in Food Research International
Elsevier., 100, 132-136.
https://doi.org/10.1016/j.foodres.2017.08.040

Martinov J, Krstic M, Spasić S, Miletić S, Stefanovic-Kojic J, Nikolić-Kokić A, Blagojevic D, Spasojević I, Spasić M. Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus. in Food Research International. 2017;100:132-136.
doi:10.1016/j.foodres.2017.08.040 .

Martinov, Jelena, Krstic, Miodrag, Spasić, Snežana, Miletić, Srđan, Stefanovic-Kojic, Jovana, Nikolić-Kokić, Aleksandra, Blagojevic, Dusko, Spasojević, Ivan, Spasić, Mihajlo, "Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus" in Food Research International, 100 (2017):132-136,
https://doi.org/10.1016/j.foodres.2017.08.040 . .

TY  - CONF
AU  - Lugonja, Nikoleta
AU  - Spasić, Snežana
AU  - Miletić, Srđan
AU  - Minić, Simeon
AU  - Nikolić Kokić, Aleksandra
AU  - Marinković, Vesna
AU  - Vrvić, Miroslav
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5529
AB  - Donated milk represents a desirable alternative to formula feeding, especially for preterm infants. Milk banks collect both preterm and term human milk and apply pasteurization to remove possible infectious contaminants. We examined the effects of pasteurization on redox properties of milk from mothers of preterm infants and its fractions. Electron paramagnetic resonance spectroscopy of hydroxyl-radical generating Fenton system was applied to analyze redox properties of milk from mothers of preterm infants and its fractions (skim milk and whey) and reactive products of oxidation of ascorbate and uric acid in the milk.
PB  - International Conferences on Nutrition & Growth
C3  - Abstract Book - 3rd International Conference on Nutrition & Growth, March 17-19, 2016, Vienna, Austria
T1  - Effects of pasteurization on redox properties of colostrum and milk from mothers of preterm infants
SP  - 98
UR  - https://hdl.handle.net/21.15107/rcub_cer_5529
ER  - 

@conference{
author = "Lugonja, Nikoleta and Spasić, Snežana and Miletić, Srđan and Minić, Simeon and Nikolić Kokić, Aleksandra and Marinković, Vesna and Vrvić, Miroslav",
year = "2016",
abstract = "Donated milk represents a desirable alternative to formula feeding, especially for preterm infants. Milk banks collect both preterm and term human milk and apply pasteurization to remove possible infectious contaminants. We examined the effects of pasteurization on redox properties of milk from mothers of preterm infants and its fractions. Electron paramagnetic resonance spectroscopy of hydroxyl-radical generating Fenton system was applied to analyze redox properties of milk from mothers of preterm infants and its fractions (skim milk and whey) and reactive products of oxidation of ascorbate and uric acid in the milk.",
publisher = "International Conferences on Nutrition & Growth",
journal = "Abstract Book - 3rd International Conference on Nutrition & Growth, March 17-19, 2016, Vienna, Austria",
title = "Effects of pasteurization on redox properties of colostrum and milk from mothers of preterm infants",
pages = "98",
url = "https://hdl.handle.net/21.15107/rcub_cer_5529"
}

Lugonja, N., Spasić, S., Miletić, S., Minić, S., Nikolić Kokić, A., Marinković, V.,& Vrvić, M.. (2016). Effects of pasteurization on redox properties of colostrum and milk from mothers of preterm infants. in Abstract Book - 3rd International Conference on Nutrition & Growth, March 17-19, 2016, Vienna, Austria
International Conferences on Nutrition & Growth., 98.
https://hdl.handle.net/21.15107/rcub_cer_5529

Lugonja N, Spasić S, Miletić S, Minić S, Nikolić Kokić A, Marinković V, Vrvić M. Effects of pasteurization on redox properties of colostrum and milk from mothers of preterm infants. in Abstract Book - 3rd International Conference on Nutrition & Growth, March 17-19, 2016, Vienna, Austria. 2016;:98.
https://hdl.handle.net/21.15107/rcub_cer_5529 .

Lugonja, Nikoleta, Spasić, Snežana, Miletić, Srđan, Minić, Simeon, Nikolić Kokić, Aleksandra, Marinković, Vesna, Vrvić, Miroslav, "Effects of pasteurization on redox properties of colostrum and milk from mothers of preterm infants" in Abstract Book - 3rd International Conference on Nutrition & Growth, March 17-19, 2016, Vienna, Austria (2016):98,
https://hdl.handle.net/21.15107/rcub_cer_5529 .

TY  - CONF
AU  - Spasić, Snežana
AU  - Miletić, Srđan
AU  - Minić, Simeon
AU  - Lugonja, Nikoleta
AU  - Marinković, Vesna
AU  - Spasojević, Ivan
AU  - Vrvić, Miroslav
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5531
AB  - Health benefits of mother's milk depend on the level of the total antioxidant capacity (TAC). In mother's milk, the TAC should refer to the sum of activities derived from active enzymatic antioxidant systems (e.g. superoxide dismutase, catalase, glutathione peroxidase etc.), non-enzymatic antioxidants, such as vitamins C and E, and the presence of other bioactive factors (e.g. lactoferrin, uric acid etc.). Measuring oxidation-reduction potential (ORP) using RedoxSYS Analyzer may be an alternative to classic methods of measuring TAC. We compared the results of ORR and ascorbic acid content in milk from mothers of preterm infants.
PB  - International Conferences on Nutrition & Growth
C3  - Abstract Book - 3rd International Conference on Nutrition & Growth, March 17-19, 2016, Vienna, Austria
T1  - Oxidation-reduction potential in the milk from mothers of preterm infants
SP  - 110
UR  - https://hdl.handle.net/21.15107/rcub_cer_5531
ER  - 

@conference{
author = "Spasić, Snežana and Miletić, Srđan and Minić, Simeon and Lugonja, Nikoleta and Marinković, Vesna and Spasojević, Ivan and Vrvić, Miroslav",
year = "2016",
abstract = "Health benefits of mother's milk depend on the level of the total antioxidant capacity (TAC). In mother's milk, the TAC should refer to the sum of activities derived from active enzymatic antioxidant systems (e.g. superoxide dismutase, catalase, glutathione peroxidase etc.), non-enzymatic antioxidants, such as vitamins C and E, and the presence of other bioactive factors (e.g. lactoferrin, uric acid etc.). Measuring oxidation-reduction potential (ORP) using RedoxSYS Analyzer may be an alternative to classic methods of measuring TAC. We compared the results of ORR and ascorbic acid content in milk from mothers of preterm infants.",
publisher = "International Conferences on Nutrition & Growth",
journal = "Abstract Book - 3rd International Conference on Nutrition & Growth, March 17-19, 2016, Vienna, Austria",
title = "Oxidation-reduction potential in the milk from mothers of preterm infants",
pages = "110",
url = "https://hdl.handle.net/21.15107/rcub_cer_5531"
}

Spasić, S., Miletić, S., Minić, S., Lugonja, N., Marinković, V., Spasojević, I.,& Vrvić, M.. (2016). Oxidation-reduction potential in the milk from mothers of preterm infants. in Abstract Book - 3rd International Conference on Nutrition & Growth, March 17-19, 2016, Vienna, Austria
International Conferences on Nutrition & Growth., 110.
https://hdl.handle.net/21.15107/rcub_cer_5531

Spasić S, Miletić S, Minić S, Lugonja N, Marinković V, Spasojević I, Vrvić M. Oxidation-reduction potential in the milk from mothers of preterm infants. in Abstract Book - 3rd International Conference on Nutrition & Growth, March 17-19, 2016, Vienna, Austria. 2016;:110.
https://hdl.handle.net/21.15107/rcub_cer_5531 .

Spasić, Snežana, Miletić, Srđan, Minić, Simeon, Lugonja, Nikoleta, Marinković, Vesna, Spasojević, Ivan, Vrvić, Miroslav, "Oxidation-reduction potential in the milk from mothers of preterm infants" in Abstract Book - 3rd International Conference on Nutrition & Growth, March 17-19, 2016, Vienna, Austria (2016):110,
https://hdl.handle.net/21.15107/rcub_cer_5531 .

TY  - CONF
AU  - Lugonja, Nikoleta
AU  - Spasić, Snežana
AU  - Miletić, Srđan
AU  - Minić, Simeon
AU  - Nikolić Kokić, Aleksandra
AU  - Marinković, Vesna
AU  - Vrvić, Miroslav
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5530
AB  - Donated milk represents a desirable alternative to formula feeding, especially for preterm infants. Milk banks collect both preterm and term human milk and apply pasteurization to remove possible infectious contaminants. We examined the effects of pasteurization on redox properties of milk from mothers of preterm infants and its fractions. Electron paramagnetic resonance spectroscopy of hydroxyl-radical generating Fenton system was applied to analyze redox properties of milk from mothers of preterm infants and its fractions (skim milk and whey) and reactive products of oxidation of ascorbate and uric acid in the milk.
T1  - Effects of pasteurization on redox properties of colostrum and milk from mothers of preterm infants
UR  - https://hdl.handle.net/21.15107/rcub_cer_5530
ER  - 

@conference{
author = "Lugonja, Nikoleta and Spasić, Snežana and Miletić, Srđan and Minić, Simeon and Nikolić Kokić, Aleksandra and Marinković, Vesna and Vrvić, Miroslav",
year = "2016",
abstract = "Donated milk represents a desirable alternative to formula feeding, especially for preterm infants. Milk banks collect both preterm and term human milk and apply pasteurization to remove possible infectious contaminants. We examined the effects of pasteurization on redox properties of milk from mothers of preterm infants and its fractions. Electron paramagnetic resonance spectroscopy of hydroxyl-radical generating Fenton system was applied to analyze redox properties of milk from mothers of preterm infants and its fractions (skim milk and whey) and reactive products of oxidation of ascorbate and uric acid in the milk.",
title = "Effects of pasteurization on redox properties of colostrum and milk from mothers of preterm infants",
url = "https://hdl.handle.net/21.15107/rcub_cer_5530"
}

Lugonja, N., Spasić, S., Miletić, S., Minić, S., Nikolić Kokić, A., Marinković, V.,& Vrvić, M.. (2016). Effects of pasteurization on redox properties of colostrum and milk from mothers of preterm infants. .
https://hdl.handle.net/21.15107/rcub_cer_5530

Lugonja N, Spasić S, Miletić S, Minić S, Nikolić Kokić A, Marinković V, Vrvić M. Effects of pasteurization on redox properties of colostrum and milk from mothers of preterm infants. 2016;.
https://hdl.handle.net/21.15107/rcub_cer_5530 .

Lugonja, Nikoleta, Spasić, Snežana, Miletić, Srđan, Minić, Simeon, Nikolić Kokić, Aleksandra, Marinković, Vesna, Vrvić, Miroslav, "Effects of pasteurization on redox properties of colostrum and milk from mothers of preterm infants" (2016),
https://hdl.handle.net/21.15107/rcub_cer_5530 .

TY  - CONF
AU  - Spasić, Snežana
AU  - Miletić, Srđan
AU  - Minić, Simeon
AU  - Lugonja, Nikoleta
AU  - Marinković, Vesna
AU  - Spasojević, Ivan
AU  - Vrvić, Miroslav
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5532
AB  - Health benefits of mother's milk depend on the level of the total antioxidant capacity (TAC). In mother's milk, the TAC should refer to the sum of activities derived from active enzymatic antioxidant systems (e.g. superoxide dismutase, catalase, glutathione peroxidase etc.), non-enzymatic antioxidants, such as vitamins C and E, and the presence of other bioactive factors (e.g. lactoferrin, uric acid etc.). Measuring oxidation-reduction potential (ORP) using RedoxSYS Analyzer may be an alternative to classic methods of measuring TAC. We compared the results of ORR and ascorbic acid content in milk from mothers of preterm infants.
T1  - Oxidation-reduction potential in the milk from mothers of preterm infants
SP  - 110
UR  - https://hdl.handle.net/21.15107/rcub_cer_5532
ER  - 

@conference{
author = "Spasić, Snežana and Miletić, Srđan and Minić, Simeon and Lugonja, Nikoleta and Marinković, Vesna and Spasojević, Ivan and Vrvić, Miroslav",
year = "2016",
abstract = "Health benefits of mother's milk depend on the level of the total antioxidant capacity (TAC). In mother's milk, the TAC should refer to the sum of activities derived from active enzymatic antioxidant systems (e.g. superoxide dismutase, catalase, glutathione peroxidase etc.), non-enzymatic antioxidants, such as vitamins C and E, and the presence of other bioactive factors (e.g. lactoferrin, uric acid etc.). Measuring oxidation-reduction potential (ORP) using RedoxSYS Analyzer may be an alternative to classic methods of measuring TAC. We compared the results of ORR and ascorbic acid content in milk from mothers of preterm infants.",
title = "Oxidation-reduction potential in the milk from mothers of preterm infants",
pages = "110",
url = "https://hdl.handle.net/21.15107/rcub_cer_5532"
}

Spasić, S., Miletić, S., Minić, S., Lugonja, N., Marinković, V., Spasojević, I.,& Vrvić, M.. (2016). Oxidation-reduction potential in the milk from mothers of preterm infants. , 110.
https://hdl.handle.net/21.15107/rcub_cer_5532

Spasić S, Miletić S, Minić S, Lugonja N, Marinković V, Spasojević I, Vrvić M. Oxidation-reduction potential in the milk from mothers of preterm infants. 2016;:110.
https://hdl.handle.net/21.15107/rcub_cer_5532 .

Spasić, Snežana, Miletić, Srđan, Minić, Simeon, Lugonja, Nikoleta, Marinković, Vesna, Spasojević, Ivan, Vrvić, Miroslav, "Oxidation-reduction potential in the milk from mothers of preterm infants" (2016):110,
https://hdl.handle.net/21.15107/rcub_cer_5532 .

TY  - JOUR
AU  - Marinković, Vesna
AU  - Rankovic-Janevski, Milica
AU  - Spasić, Snežana
AU  - Nikolić-Kokić, Aleksandra
AU  - Lugonja, Nikoleta
AU  - Đurović, Dijana
AU  - Miletić, Srđan
AU  - Vrvić, Miroslav
AU  - Spasojević, Ivan
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2025
AB  - Objectives: Milk banks collect, pasteurize, and freeze/store human milk. The processing may alter redox properties of milk, but the effects have not been fully examined. Methods: We collected 10 mature milk and 10 colostrum samples and applied a battery of biochemical assays and electron paramagnetic resonance spectroscopy to inspect changes that milk undergoes with pasteurization and 30 days storage at -20 degrees C. Results: Pasteurization and storage of raw milk did not affect total nonenzymatic antioxidative capacity, but specific components and features were altered. Urate radical and ascorbyl radical emerge as products of exposure of milk to hydroxyl radical-generating system. Processing shifted the load of antioxidative activity from ascorbate to urate and lowered the capacity of milk to diminish hydroxyl radical. Pasteurization caused a significant drop in the activity of 2 major antioxidative enzymes-superoxide dismutase and glutathione peroxidase, whereas freezing/storage of raw milk affected only superoxide dismutase. Colostrum showed drastically higher total nonenzymatic antioxidative capacity, hydroxyl radical scavenging ability, and glutathione reductase activity compared with mature milk. Conclusions: Pasteurization and storage affect nonenzymatic and enzymatic antioxidative agents in human milk. It appears that nonenzymatic antioxidative systems in colostrum and milk are different. The effects of processing may be partially compensated by fortification/spiking with ascorbate before use.
PB  - Lippincott Williams & Wilkins, Philadelphia
T2  - Journal of Pediatric Gastroenterology and Nutrition
T1  - Antioxidative Activity of Colostrum and Human Milk: Effects of Pasteurization and Storage
VL  - 62
IS  - 6
SP  - 901
EP  - 906
DO  - 10.1097/MPG.0000000000001090
ER  - 

@article{
author = "Marinković, Vesna and Rankovic-Janevski, Milica and Spasić, Snežana and Nikolić-Kokić, Aleksandra and Lugonja, Nikoleta and Đurović, Dijana and Miletić, Srđan and Vrvić, Miroslav and Spasojević, Ivan",
year = "2016",
abstract = "Objectives: Milk banks collect, pasteurize, and freeze/store human milk. The processing may alter redox properties of milk, but the effects have not been fully examined. Methods: We collected 10 mature milk and 10 colostrum samples and applied a battery of biochemical assays and electron paramagnetic resonance spectroscopy to inspect changes that milk undergoes with pasteurization and 30 days storage at -20 degrees C. Results: Pasteurization and storage of raw milk did not affect total nonenzymatic antioxidative capacity, but specific components and features were altered. Urate radical and ascorbyl radical emerge as products of exposure of milk to hydroxyl radical-generating system. Processing shifted the load of antioxidative activity from ascorbate to urate and lowered the capacity of milk to diminish hydroxyl radical. Pasteurization caused a significant drop in the activity of 2 major antioxidative enzymes-superoxide dismutase and glutathione peroxidase, whereas freezing/storage of raw milk affected only superoxide dismutase. Colostrum showed drastically higher total nonenzymatic antioxidative capacity, hydroxyl radical scavenging ability, and glutathione reductase activity compared with mature milk. Conclusions: Pasteurization and storage affect nonenzymatic and enzymatic antioxidative agents in human milk. It appears that nonenzymatic antioxidative systems in colostrum and milk are different. The effects of processing may be partially compensated by fortification/spiking with ascorbate before use.",
publisher = "Lippincott Williams & Wilkins, Philadelphia",
journal = "Journal of Pediatric Gastroenterology and Nutrition",
title = "Antioxidative Activity of Colostrum and Human Milk: Effects of Pasteurization and Storage",
volume = "62",
number = "6",
pages = "901-906",
doi = "10.1097/MPG.0000000000001090"
}

Marinković, V., Rankovic-Janevski, M., Spasić, S., Nikolić-Kokić, A., Lugonja, N., Đurović, D., Miletić, S., Vrvić, M.,& Spasojević, I.. (2016). Antioxidative Activity of Colostrum and Human Milk: Effects of Pasteurization and Storage. in Journal of Pediatric Gastroenterology and Nutrition
Lippincott Williams & Wilkins, Philadelphia., 62(6), 901-906.
https://doi.org/10.1097/MPG.0000000000001090

Marinković V, Rankovic-Janevski M, Spasić S, Nikolić-Kokić A, Lugonja N, Đurović D, Miletić S, Vrvić M, Spasojević I. Antioxidative Activity of Colostrum and Human Milk: Effects of Pasteurization and Storage. in Journal of Pediatric Gastroenterology and Nutrition. 2016;62(6):901-906.
doi:10.1097/MPG.0000000000001090 .

Marinković, Vesna, Rankovic-Janevski, Milica, Spasić, Snežana, Nikolić-Kokić, Aleksandra, Lugonja, Nikoleta, Đurović, Dijana, Miletić, Srđan, Vrvić, Miroslav, Spasojević, Ivan, "Antioxidative Activity of Colostrum and Human Milk: Effects of Pasteurization and Storage" in Journal of Pediatric Gastroenterology and Nutrition, 62, no. 6 (2016):901-906,
https://doi.org/10.1097/MPG.0000000000001090 . .

TY  - JOUR
AU  - Bolic, Bojana
AU  - Mijuskovic, Ana
AU  - Popovic-Bijelic, Ana
AU  - Nikolić-Kokić, Aleksandra
AU  - Spasić, Snežana
AU  - Blagojevic, Dusko
AU  - Spasić, Mihajlo
AU  - Spasojević, Ivan
PY  - 2015
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1787
AB  - Interactions of hydrogen sulfide (HS-/H2S), a reducing signaling species, with superoxide dimutases (SOD) are poorly understood. We applied low-T EPR spectroscopy to examine the effects of HS-/H2S and superoxide radical anion (O-2(-)) on metallocenters of FeSOD, MnSOD, and CuZnSOD. HS-/H2S did not affect FeSOD, whereas active centers of MnSOD and CuZnSOD were open to this agent. Cu2+ was reduced to Cu1+, while manganese appears to be released from MnSOD active center. Untreated and O-2(-) treated FeSOD and MnSOD predominantly show 5 d-electron systems, i.e. Fe3+ and Mn2+. Our study provides new details on the mechanisms of (patho)physiological effects of HS-/H2S.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Nitric Oxide-Biology and Chemistry
T1  - Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study
VL  - 51
SP  - 19
EP  - 23
DO  - 10.1016/j.niox.2015.09.008
ER  - 

@article{
author = "Bolic, Bojana and Mijuskovic, Ana and Popovic-Bijelic, Ana and Nikolić-Kokić, Aleksandra and Spasić, Snežana and Blagojevic, Dusko and Spasić, Mihajlo and Spasojević, Ivan",
year = "2015",
abstract = "Interactions of hydrogen sulfide (HS-/H2S), a reducing signaling species, with superoxide dimutases (SOD) are poorly understood. We applied low-T EPR spectroscopy to examine the effects of HS-/H2S and superoxide radical anion (O-2(-)) on metallocenters of FeSOD, MnSOD, and CuZnSOD. HS-/H2S did not affect FeSOD, whereas active centers of MnSOD and CuZnSOD were open to this agent. Cu2+ was reduced to Cu1+, while manganese appears to be released from MnSOD active center. Untreated and O-2(-) treated FeSOD and MnSOD predominantly show 5 d-electron systems, i.e. Fe3+ and Mn2+. Our study provides new details on the mechanisms of (patho)physiological effects of HS-/H2S.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Nitric Oxide-Biology and Chemistry",
title = "Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study",
volume = "51",
pages = "19-23",
doi = "10.1016/j.niox.2015.09.008"
}

Bolic, B., Mijuskovic, A., Popovic-Bijelic, A., Nikolić-Kokić, A., Spasić, S., Blagojevic, D., Spasić, M.,& Spasojević, I.. (2015). Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study. in Nitric Oxide-Biology and Chemistry
Academic Press Inc Elsevier Science, San Diego., 51, 19-23.
https://doi.org/10.1016/j.niox.2015.09.008

Bolic B, Mijuskovic A, Popovic-Bijelic A, Nikolić-Kokić A, Spasić S, Blagojevic D, Spasić M, Spasojević I. Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study. in Nitric Oxide-Biology and Chemistry. 2015;51:19-23.
doi:10.1016/j.niox.2015.09.008 .

Bolic, Bojana, Mijuskovic, Ana, Popovic-Bijelic, Ana, Nikolić-Kokić, Aleksandra, Spasić, Snežana, Blagojevic, Dusko, Spasić, Mihajlo, Spasojević, Ivan, "Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study" in Nitric Oxide-Biology and Chemistry, 51 (2015):19-23,
https://doi.org/10.1016/j.niox.2015.09.008 . .

TY  - JOUR
AU  - Bolic, Bojana
AU  - Mijuskovic, Ana
AU  - Popovic-Bijelic, Ana
AU  - Nikolić-Kokić, Aleksandra
AU  - Spasić, Snežana
AU  - Blagojevic, Dusko
AU  - Spasić, Mihajlo
AU  - Spasojević, Ivan
PY  - 2015
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3187
AB  - Interactions of hydrogen sulfide (HS-/H2S), a reducing signaling species, with superoxide dimutases (SOD) are poorly understood. We applied low-T EPR spectroscopy to examine the effects of HS-/H2S and superoxide radical anion (O-2(-)) on metallocenters of FeSOD, MnSOD, and CuZnSOD. HS-/H2S did not affect FeSOD, whereas active centers of MnSOD and CuZnSOD were open to this agent. Cu2+ was reduced to Cu1+, while manganese appears to be released from MnSOD active center. Untreated and O-2(-) treated FeSOD and MnSOD predominantly show 5 d-electron systems, i.e. Fe3+ and Mn2+. Our study provides new details on the mechanisms of (patho)physiological effects of HS-/H2S.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Nitric Oxide-Biology and Chemistry
T1  - Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study
VL  - 51
SP  - 19
EP  - 23
DO  - 10.1016/j.niox.2015.09.008
ER  - 

@article{
author = "Bolic, Bojana and Mijuskovic, Ana and Popovic-Bijelic, Ana and Nikolić-Kokić, Aleksandra and Spasić, Snežana and Blagojevic, Dusko and Spasić, Mihajlo and Spasojević, Ivan",
year = "2015",
abstract = "Interactions of hydrogen sulfide (HS-/H2S), a reducing signaling species, with superoxide dimutases (SOD) are poorly understood. We applied low-T EPR spectroscopy to examine the effects of HS-/H2S and superoxide radical anion (O-2(-)) on metallocenters of FeSOD, MnSOD, and CuZnSOD. HS-/H2S did not affect FeSOD, whereas active centers of MnSOD and CuZnSOD were open to this agent. Cu2+ was reduced to Cu1+, while manganese appears to be released from MnSOD active center. Untreated and O-2(-) treated FeSOD and MnSOD predominantly show 5 d-electron systems, i.e. Fe3+ and Mn2+. Our study provides new details on the mechanisms of (patho)physiological effects of HS-/H2S.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Nitric Oxide-Biology and Chemistry",
title = "Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study",
volume = "51",
pages = "19-23",
doi = "10.1016/j.niox.2015.09.008"
}

Bolic, B., Mijuskovic, A., Popovic-Bijelic, A., Nikolić-Kokić, A., Spasić, S., Blagojevic, D., Spasić, M.,& Spasojević, I.. (2015). Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study. in Nitric Oxide-Biology and Chemistry
Academic Press Inc Elsevier Science, San Diego., 51, 19-23.
https://doi.org/10.1016/j.niox.2015.09.008

Bolic B, Mijuskovic A, Popovic-Bijelic A, Nikolić-Kokić A, Spasić S, Blagojevic D, Spasić M, Spasojević I. Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study. in Nitric Oxide-Biology and Chemistry. 2015;51:19-23.
doi:10.1016/j.niox.2015.09.008 .

Bolic, Bojana, Mijuskovic, Ana, Popovic-Bijelic, Ana, Nikolić-Kokić, Aleksandra, Spasić, Snežana, Blagojevic, Dusko, Spasić, Mihajlo, Spasojević, Ivan, "Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study" in Nitric Oxide-Biology and Chemistry, 51 (2015):19-23,
https://doi.org/10.1016/j.niox.2015.09.008 . .

TY  - JOUR
AU  - Ristic, Aleksandar J.
AU  - Sokic, Dragoslav
AU  - Baščarević, Vladimir
AU  - Spasić, Snežana
AU  - Vojvodic, Nikola
AU  - Savić, Slobodan
AU  - Raičević, Savo
AU  - Kovacevic, Masa
AU  - Savic, Danijela
AU  - Spasojević, Ivan
PY  - 2014
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1583
AB  - An altered metal and electrolyte profile has been implicated in the pathologic mechanisms of chronic epilepsy; however, no study has comprehensively measured hippocampal concentrations of these elements in patients with mesial temporal lobe epilepsy and hippocampal sclerosis (mTLE-HS). We therefore analyzed hippocampi of 24 patients with drug-resistant mTLE-HS (mean age 35.69.4years) who underwent anterior temporal lobe resection and amygdalohippocampectomy and 17 hippocampi obtained by autopsy from 13 controls (mean age 40.5 +/- 12.9years), using inductively coupled plasma optical emission spectrometry (ICP-OES). Epileptic hippocampi showed significantly lower concentrations (g/g of tissue) of copper (HS: 2.34 +/- 0.12; control [C]: 3.57 +/- 0.33; p LT 0.001), manganese (HS: 0.205 +/- 0.030; C: 0.409 +/- 0.064; p=0.004), and potassium (HS: 2,001 +/- 59; C: 2,322 +/- 61; p LT 0.001), and increased sodium levels (HS: 1,131 +/- 22; C: 1,040 +/- 25; p=0.010). Zinc, iron, calcium, and magnesium levels did not differ in HS and controls. In summary, copper and manganese levels are deficient, whereas iron level is unchanged in hippocampi from patients with mTLE-HS. Our results provide a basis for understanding the potential involvement of different metals and electrolytes in the pathology of HS. A PowerPoint slide summarizing this article is available for download in the Supporting Information section .
PB  - Wiley-Blackwell, Hoboken
T2  - Epilepsia
T1  - Metals and electrolytes in sclerotic hippocampi in patients with drug-resistant mesial temporal lobe epilepsy
VL  - 55
IS  - 5
SP  - e34
EP  - e37
DO  - 10.1111/epi.12593
ER  - 

@article{
author = "Ristic, Aleksandar J. and Sokic, Dragoslav and Baščarević, Vladimir and Spasić, Snežana and Vojvodic, Nikola and Savić, Slobodan and Raičević, Savo and Kovacevic, Masa and Savic, Danijela and Spasojević, Ivan",
year = "2014",
abstract = "An altered metal and electrolyte profile has been implicated in the pathologic mechanisms of chronic epilepsy; however, no study has comprehensively measured hippocampal concentrations of these elements in patients with mesial temporal lobe epilepsy and hippocampal sclerosis (mTLE-HS). We therefore analyzed hippocampi of 24 patients with drug-resistant mTLE-HS (mean age 35.69.4years) who underwent anterior temporal lobe resection and amygdalohippocampectomy and 17 hippocampi obtained by autopsy from 13 controls (mean age 40.5 +/- 12.9years), using inductively coupled plasma optical emission spectrometry (ICP-OES). Epileptic hippocampi showed significantly lower concentrations (g/g of tissue) of copper (HS: 2.34 +/- 0.12; control [C]: 3.57 +/- 0.33; p LT 0.001), manganese (HS: 0.205 +/- 0.030; C: 0.409 +/- 0.064; p=0.004), and potassium (HS: 2,001 +/- 59; C: 2,322 +/- 61; p LT 0.001), and increased sodium levels (HS: 1,131 +/- 22; C: 1,040 +/- 25; p=0.010). Zinc, iron, calcium, and magnesium levels did not differ in HS and controls. In summary, copper and manganese levels are deficient, whereas iron level is unchanged in hippocampi from patients with mTLE-HS. Our results provide a basis for understanding the potential involvement of different metals and electrolytes in the pathology of HS. A PowerPoint slide summarizing this article is available for download in the Supporting Information section .",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Epilepsia",
title = "Metals and electrolytes in sclerotic hippocampi in patients with drug-resistant mesial temporal lobe epilepsy",
volume = "55",
number = "5",
pages = "e34-e37",
doi = "10.1111/epi.12593"
}

Ristic, A. J., Sokic, D., Baščarević, V., Spasić, S., Vojvodic, N., Savić, S., Raičević, S., Kovacevic, M., Savic, D.,& Spasojević, I.. (2014). Metals and electrolytes in sclerotic hippocampi in patients with drug-resistant mesial temporal lobe epilepsy. in Epilepsia
Wiley-Blackwell, Hoboken., 55(5), e34-e37.
https://doi.org/10.1111/epi.12593

Ristic AJ, Sokic D, Baščarević V, Spasić S, Vojvodic N, Savić S, Raičević S, Kovacevic M, Savic D, Spasojević I. Metals and electrolytes in sclerotic hippocampi in patients with drug-resistant mesial temporal lobe epilepsy. in Epilepsia. 2014;55(5):e34-e37.
doi:10.1111/epi.12593 .

Ristic, Aleksandar J., Sokic, Dragoslav, Baščarević, Vladimir, Spasić, Snežana, Vojvodic, Nikola, Savić, Slobodan, Raičević, Savo, Kovacevic, Masa, Savic, Danijela, Spasojević, Ivan, "Metals and electrolytes in sclerotic hippocampi in patients with drug-resistant mesial temporal lobe epilepsy" in Epilepsia, 55, no. 5 (2014):e34-e37,
https://doi.org/10.1111/epi.12593 . .

TY  - JOUR
AU  - Bajcetic, Milica
AU  - Spasić, Snežana
AU  - Spasojević, Ivan
PY  - 2014
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1433
UR  - https://journals.lww.com/shockjournal/Fulltext/2014/09000/Redox_Therapy_in_Neonatal_Sepsis___Reasons,.2.aspx#pdf-link
AB  - Neonatal sepsis is one of the most fulminating conditions in neonatal intensive care units. Antipathogen and supportive care are administered routinely, but do not deliver satisfactory results. In addition, the efforts to treat neonatal sepsis with anti-inflammatory agents have generally shown to be futile. The accumulating data imply that intracellular redox changes intertwined into neonatal sepsis redox cycle represent the main cause of dysfunction of mitochondria and cells in neonatal sepsis. Our aim here is to support the new philosophy in neonatal sepsis treatment, which involves the integration of mechanisms that are responsible for cellular dysfunction and organ failure, the recognition of the most important targets, and the selection of safe agents that can stop the neonatal sepsis redox cycle by hitting the hot spots. Redox-active agents that could be beneficial for neonatal sepsis treatment according to these criteria include lactoferrin, interleukin 10, zinc and selenium supplements, ibuprofen, edaravone, and pentoxifylline.
PB  - Lippincott Williams & Wilkins, Philadelphia
T2  - Shock
T1  - Redox therapy in neonatal sepsis: reasons, targets, strategy, and agents
VL  - 42
IS  - 3
SP  - 179
EP  - 184
DO  - 10.1097/SHK.0000000000000198
ER  - 

@article{
author = "Bajcetic, Milica and Spasić, Snežana and Spasojević, Ivan",
year = "2014",
abstract = "Neonatal sepsis is one of the most fulminating conditions in neonatal intensive care units. Antipathogen and supportive care are administered routinely, but do not deliver satisfactory results. In addition, the efforts to treat neonatal sepsis with anti-inflammatory agents have generally shown to be futile. The accumulating data imply that intracellular redox changes intertwined into neonatal sepsis redox cycle represent the main cause of dysfunction of mitochondria and cells in neonatal sepsis. Our aim here is to support the new philosophy in neonatal sepsis treatment, which involves the integration of mechanisms that are responsible for cellular dysfunction and organ failure, the recognition of the most important targets, and the selection of safe agents that can stop the neonatal sepsis redox cycle by hitting the hot spots. Redox-active agents that could be beneficial for neonatal sepsis treatment according to these criteria include lactoferrin, interleukin 10, zinc and selenium supplements, ibuprofen, edaravone, and pentoxifylline.",
publisher = "Lippincott Williams & Wilkins, Philadelphia",
journal = "Shock",
title = "Redox therapy in neonatal sepsis: reasons, targets, strategy, and agents",
volume = "42",
number = "3",
pages = "179-184",
doi = "10.1097/SHK.0000000000000198"
}

Bajcetic, M., Spasić, S.,& Spasojević, I.. (2014). Redox therapy in neonatal sepsis: reasons, targets, strategy, and agents. in Shock
Lippincott Williams & Wilkins, Philadelphia., 42(3), 179-184.
https://doi.org/10.1097/SHK.0000000000000198

Bajcetic M, Spasić S, Spasojević I. Redox therapy in neonatal sepsis: reasons, targets, strategy, and agents. in Shock. 2014;42(3):179-184.
doi:10.1097/SHK.0000000000000198 .

Bajcetic, Milica, Spasić, Snežana, Spasojević, Ivan, "Redox therapy in neonatal sepsis: reasons, targets, strategy, and agents" in Shock, 42, no. 3 (2014):179-184,
https://doi.org/10.1097/SHK.0000000000000198 . .

TY  - JOUR
AU  - Bajcetic, Milica
AU  - Otasevic, Biljana
AU  - Prekajski, Niveska Bozinovic
AU  - Spasić, Snežana
AU  - Spasojević, Ivan
PY  - 2014
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1416
AB  - Background: Vitamin E is routinely supplemented to preterm babies, including those with neonatal sepsis. Our aim was to examine the effects of neonatal sepsis and vitamin E on antioxidative system (AOS) in the blood. Methods: A prospective, randomized, open label study involved 65 preterm neonates (control/sepsis - 34/31), which were divided into two subgroups - non-supplemented and supplemented with vitamin E (25 IU/day for 60 days). The activities of superoxide dismutase, catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) were determined in erythrocytes at days 0, 30, and 60, following sepsis diagnosis. Results: There was no difference in the activity of AOS between controls and neonates with ongoing sepsis. At 60 days, septic neonates showed higher CAT activity compared to controls (P = 0.027), and lower GPx activity compared to 0 days (P = 0.022). The later was mitigated by vitamin E, which on the other hand provoked lower GPx activity at 30 days, compared to untreated septic neonates (P = 0.014). In addition, vitamin E suppressed GR activity in septic neonates (P = 0.025 and P = 0.017 at 30 and 60 days). Finally, vitamin E supplementation in control neonates provoked a significant increase of GPx activity (P = 0.015 at 60 days). Conclusions: The absence of altered redox settings in the blood of neonates during sepsis episode, and vitamin E-provoked decrease in the activity of some components of AOS, suggest that the supplementation of vitamin E in these patients might not be rational.
PB  - Sage Publications Inc, Thousand Oaks
T2  - Annals of Clinical Biochemistry
T1  - Antioxidative system in the erythrocytes of preterm neonates with sepsis: the effects of vitamin E supplementation
VL  - 51
IS  - 5
SP  - 550
EP  - 556
DO  - 10.1177/0004563213503317
ER  - 

@article{
author = "Bajcetic, Milica and Otasevic, Biljana and Prekajski, Niveska Bozinovic and Spasić, Snežana and Spasojević, Ivan",
year = "2014",
abstract = "Background: Vitamin E is routinely supplemented to preterm babies, including those with neonatal sepsis. Our aim was to examine the effects of neonatal sepsis and vitamin E on antioxidative system (AOS) in the blood. Methods: A prospective, randomized, open label study involved 65 preterm neonates (control/sepsis - 34/31), which were divided into two subgroups - non-supplemented and supplemented with vitamin E (25 IU/day for 60 days). The activities of superoxide dismutase, catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) were determined in erythrocytes at days 0, 30, and 60, following sepsis diagnosis. Results: There was no difference in the activity of AOS between controls and neonates with ongoing sepsis. At 60 days, septic neonates showed higher CAT activity compared to controls (P = 0.027), and lower GPx activity compared to 0 days (P = 0.022). The later was mitigated by vitamin E, which on the other hand provoked lower GPx activity at 30 days, compared to untreated septic neonates (P = 0.014). In addition, vitamin E suppressed GR activity in septic neonates (P = 0.025 and P = 0.017 at 30 and 60 days). Finally, vitamin E supplementation in control neonates provoked a significant increase of GPx activity (P = 0.015 at 60 days). Conclusions: The absence of altered redox settings in the blood of neonates during sepsis episode, and vitamin E-provoked decrease in the activity of some components of AOS, suggest that the supplementation of vitamin E in these patients might not be rational.",
publisher = "Sage Publications Inc, Thousand Oaks",
journal = "Annals of Clinical Biochemistry",
title = "Antioxidative system in the erythrocytes of preterm neonates with sepsis: the effects of vitamin E supplementation",
volume = "51",
number = "5",
pages = "550-556",
doi = "10.1177/0004563213503317"
}

Bajcetic, M., Otasevic, B., Prekajski, N. B., Spasić, S.,& Spasojević, I.. (2014). Antioxidative system in the erythrocytes of preterm neonates with sepsis: the effects of vitamin E supplementation. in Annals of Clinical Biochemistry
Sage Publications Inc, Thousand Oaks., 51(5), 550-556.
https://doi.org/10.1177/0004563213503317

Bajcetic M, Otasevic B, Prekajski NB, Spasić S, Spasojević I. Antioxidative system in the erythrocytes of preterm neonates with sepsis: the effects of vitamin E supplementation. in Annals of Clinical Biochemistry. 2014;51(5):550-556.
doi:10.1177/0004563213503317 .

Bajcetic, Milica, Otasevic, Biljana, Prekajski, Niveska Bozinovic, Spasić, Snežana, Spasojević, Ivan, "Antioxidative system in the erythrocytes of preterm neonates with sepsis: the effects of vitamin E supplementation" in Annals of Clinical Biochemistry, 51, no. 5 (2014):550-556,
https://doi.org/10.1177/0004563213503317 . .

TY  - JOUR
AU  - Lugonja, Nikoleta
AU  - Spasić, Snežana
AU  - Laugier, Olga B.
AU  - Nikolić-Kokić, Aleksandra
AU  - Spasojević, Ivan
AU  - Oreščanin-Dušić, Zorana
AU  - Vrvić, Miroslav
PY  - 2013
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1238
AB  - Objective: Early-onset and exclusive breast-feeding provides a significant health benefit to infants compared with infant formulas. The aim of this study was to compare mature breast milk with standard infant formulas by examining their effects on non-vascular smooth muscle contraction and their antioxidative properties. Methods: The pharmacologic effects of breast milk and formulas were examined using a model system of the rat uterine smooth muscle contraction. Electron paramagnetic resonance spin-trapping spectroscopy was used to compare the antioxidative capacities of breast milk (obtained in the ninth week of lactation) with commercial infant formulas against hydroxyl radical production in the Fenton reaction. The activities of superoxide dismutase, glutathione peroxidase, and the sulfhydryl group were determined in the breast milk and infant formulas. Results: In contrast to the infant formulas, breast milk exerted a relaxing effect on isolated non-vascular smooth muscle. In general, breast milk showed higher antioxidative activity compared with the infant formulas. In all samples, the generation of hydroxyl radicals led to the formation of carbon-centered and ascorbyl radicals. Conclusions: Human milk exerts direct pharmacologic relaxation effects and provides better antioxidant protection compared with infant formulas because of the presence of specific enzymatic components, such as human superoxide dismutase. We propose that these effects should be advantageous to an infant's gastrointestinal tract by supporting the normal work of the smooth musculature and maintaining redox homeostasis and may represent one of the mechanisms by which breast-feeding benefits health.
PB  - Elsevier Science Inc, New York
T2  - Nutrition
T1  - Differences in direct pharmacologic effects and antioxidative properties of mature breast milk and infant formulas
VL  - 29
IS  - 2
SP  - 431
EP  - 435
DO  - 10.1016/j.nut.2012.07.018
ER  - 

@article{
author = "Lugonja, Nikoleta and Spasić, Snežana and Laugier, Olga B. and Nikolić-Kokić, Aleksandra and Spasojević, Ivan and Oreščanin-Dušić, Zorana and Vrvić, Miroslav",
year = "2013",
abstract = "Objective: Early-onset and exclusive breast-feeding provides a significant health benefit to infants compared with infant formulas. The aim of this study was to compare mature breast milk with standard infant formulas by examining their effects on non-vascular smooth muscle contraction and their antioxidative properties. Methods: The pharmacologic effects of breast milk and formulas were examined using a model system of the rat uterine smooth muscle contraction. Electron paramagnetic resonance spin-trapping spectroscopy was used to compare the antioxidative capacities of breast milk (obtained in the ninth week of lactation) with commercial infant formulas against hydroxyl radical production in the Fenton reaction. The activities of superoxide dismutase, glutathione peroxidase, and the sulfhydryl group were determined in the breast milk and infant formulas. Results: In contrast to the infant formulas, breast milk exerted a relaxing effect on isolated non-vascular smooth muscle. In general, breast milk showed higher antioxidative activity compared with the infant formulas. In all samples, the generation of hydroxyl radicals led to the formation of carbon-centered and ascorbyl radicals. Conclusions: Human milk exerts direct pharmacologic relaxation effects and provides better antioxidant protection compared with infant formulas because of the presence of specific enzymatic components, such as human superoxide dismutase. We propose that these effects should be advantageous to an infant's gastrointestinal tract by supporting the normal work of the smooth musculature and maintaining redox homeostasis and may represent one of the mechanisms by which breast-feeding benefits health.",
publisher = "Elsevier Science Inc, New York",
journal = "Nutrition",
title = "Differences in direct pharmacologic effects and antioxidative properties of mature breast milk and infant formulas",
volume = "29",
number = "2",
pages = "431-435",
doi = "10.1016/j.nut.2012.07.018"
}

Lugonja, N., Spasić, S., Laugier, O. B., Nikolić-Kokić, A., Spasojević, I., Oreščanin-Dušić, Z.,& Vrvić, M.. (2013). Differences in direct pharmacologic effects and antioxidative properties of mature breast milk and infant formulas. in Nutrition
Elsevier Science Inc, New York., 29(2), 431-435.
https://doi.org/10.1016/j.nut.2012.07.018

Lugonja N, Spasić S, Laugier OB, Nikolić-Kokić A, Spasojević I, Oreščanin-Dušić Z, Vrvić M. Differences in direct pharmacologic effects and antioxidative properties of mature breast milk and infant formulas. in Nutrition. 2013;29(2):431-435.
doi:10.1016/j.nut.2012.07.018 .

Lugonja, Nikoleta, Spasić, Snežana, Laugier, Olga B., Nikolić-Kokić, Aleksandra, Spasojević, Ivan, Oreščanin-Dušić, Zorana, Vrvić, Miroslav, "Differences in direct pharmacologic effects and antioxidative properties of mature breast milk and infant formulas" in Nutrition, 29, no. 2 (2013):431-435,
https://doi.org/10.1016/j.nut.2012.07.018 . .

TY  - CONF
AU  - Spasić, Snežana
AU  - Lugonja, Nikoleta
AU  - Laugier, Olga B.
AU  - Oreščanin-Dušić, Zorana
AU  - Nikolić-Kokić, Aleksandra
AU  - Spasojević, Ivan
AU  - Vrvić, Miroslav
PY  - 2012
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5344
AB  - Poster presented at: 6th European Congress of Pharamcology “EPHAR 2012”
T1  - Differences in direct pharmacological effects and antioxidative properties of mature breast milk and an infant formula
UR  - https://hdl.handle.net/21.15107/rcub_cer_5344
ER  - 

@conference{
author = "Spasić, Snežana and Lugonja, Nikoleta and Laugier, Olga B. and Oreščanin-Dušić, Zorana and Nikolić-Kokić, Aleksandra and Spasojević, Ivan and Vrvić, Miroslav",
year = "2012",
abstract = "Poster presented at: 6th European Congress of Pharamcology “EPHAR 2012”",
title = "Differences in direct pharmacological effects and antioxidative properties of mature breast milk and an infant formula",
url = "https://hdl.handle.net/21.15107/rcub_cer_5344"
}

Spasić, S., Lugonja, N., Laugier, O. B., Oreščanin-Dušić, Z., Nikolić-Kokić, A., Spasojević, I.,& Vrvić, M.. (2012). Differences in direct pharmacological effects and antioxidative properties of mature breast milk and an infant formula. .
https://hdl.handle.net/21.15107/rcub_cer_5344

Spasić S, Lugonja N, Laugier OB, Oreščanin-Dušić Z, Nikolić-Kokić A, Spasojević I, Vrvić M. Differences in direct pharmacological effects and antioxidative properties of mature breast milk and an infant formula. 2012;.
https://hdl.handle.net/21.15107/rcub_cer_5344 .

Spasić, Snežana, Lugonja, Nikoleta, Laugier, Olga B., Oreščanin-Dušić, Zorana, Nikolić-Kokić, Aleksandra, Spasojević, Ivan, Vrvić, Miroslav, "Differences in direct pharmacological effects and antioxidative properties of mature breast milk and an infant formula" (2012),
https://hdl.handle.net/21.15107/rcub_cer_5344 .

TY  - CONF
AU  - Spasić, Snežana
AU  - Lugonja, Nikoleta
AU  - Laugier, Olga B.
AU  - Oreščanin-Dušić, Zorana
AU  - Nikolić-Kokić, Aleksandra
AU  - Spasojević, Ivan
AU  - Vrvić, Miroslav
PY  - 2012
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5343
AB  - Early onset and exclusive breastfeeding provides a significant health benefit to infants in comparison to infant formulas. There is groving evidence that presence of some specific protein components in mothers milk are responsible for observed significant health benefit in infants feed with mothers milk. The aim of this paper was to compare mature breast milk and a standard infant formula by examining their effects on smooth muscle contraction and their antioxidative properties. Electron paramagnetic resonance (EPR) spin-trapping spectroscopy was used to compare the antioxidative capacities of breast milk (obtained on the 9th week of lactation) with a commercial infant formula against hydroxyl radical production in the Fenton reaction. The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and their vitamin C and sulfhydryl group (–SH) contents were determined in the milks. Pharmacological research was performed on the isolated rat uterus. In contrast to the infant formula, breast milk exerted a
relaxing effect on isolated non-vascular smooth muscle. Using EPR and the Fenton reaction as a radical-generating system, we showed that breast milk possesses a three-fold higher antioxidative activity against the hydroxyl radical compared to the infant formula. In both samples, generation of the hydroxyl radical (•OH) led to the production of carbon-centered radicals. The ascorbyl radical was detected in breast milk but not in the infant formula. Human milk has direct pharmacological effects and provides better antioxidant protection than the infant formula due to the presence of specific protein components such as human SOD.
PB  - 6th European Congress of Pharamcology “EPHAR 2012”
C3  - 6th European Congress of Pharamcology “EPHAR 2012”, Granada, Spain, July 17-23 2012
T1  - Differences in direct pharmacological effects and antioxidative properties of mature breast milk and an infant formula
SP  - 351
UR  - https://hdl.handle.net/21.15107/rcub_cer_5343
ER  - 

@conference{
author = "Spasić, Snežana and Lugonja, Nikoleta and Laugier, Olga B. and Oreščanin-Dušić, Zorana and Nikolić-Kokić, Aleksandra and Spasojević, Ivan and Vrvić, Miroslav",
year = "2012",
abstract = "Early onset and exclusive breastfeeding provides a significant health benefit to infants in comparison to infant formulas. There is groving evidence that presence of some specific protein components in mothers milk are responsible for observed significant health benefit in infants feed with mothers milk. The aim of this paper was to compare mature breast milk and a standard infant formula by examining their effects on smooth muscle contraction and their antioxidative properties. Electron paramagnetic resonance (EPR) spin-trapping spectroscopy was used to compare the antioxidative capacities of breast milk (obtained on the 9th week of lactation) with a commercial infant formula against hydroxyl radical production in the Fenton reaction. The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and their vitamin C and sulfhydryl group (–SH) contents were determined in the milks. Pharmacological research was performed on the isolated rat uterus. In contrast to the infant formula, breast milk exerted a
relaxing effect on isolated non-vascular smooth muscle. Using EPR and the Fenton reaction as a radical-generating system, we showed that breast milk possesses a three-fold higher antioxidative activity against the hydroxyl radical compared to the infant formula. In both samples, generation of the hydroxyl radical (•OH) led to the production of carbon-centered radicals. The ascorbyl radical was detected in breast milk but not in the infant formula. Human milk has direct pharmacological effects and provides better antioxidant protection than the infant formula due to the presence of specific protein components such as human SOD.",
publisher = "6th European Congress of Pharamcology “EPHAR 2012”",
journal = "6th European Congress of Pharamcology “EPHAR 2012”, Granada, Spain, July 17-23 2012",
title = "Differences in direct pharmacological effects and antioxidative properties of mature breast milk and an infant formula",
pages = "351",
url = "https://hdl.handle.net/21.15107/rcub_cer_5343"
}

Spasić, S., Lugonja, N., Laugier, O. B., Oreščanin-Dušić, Z., Nikolić-Kokić, A., Spasojević, I.,& Vrvić, M.. (2012). Differences in direct pharmacological effects and antioxidative properties of mature breast milk and an infant formula. in 6th European Congress of Pharamcology “EPHAR 2012”, Granada, Spain, July 17-23 2012
6th European Congress of Pharamcology “EPHAR 2012”., 351.
https://hdl.handle.net/21.15107/rcub_cer_5343

Spasić S, Lugonja N, Laugier OB, Oreščanin-Dušić Z, Nikolić-Kokić A, Spasojević I, Vrvić M. Differences in direct pharmacological effects and antioxidative properties of mature breast milk and an infant formula. in 6th European Congress of Pharamcology “EPHAR 2012”, Granada, Spain, July 17-23 2012. 2012;:351.
https://hdl.handle.net/21.15107/rcub_cer_5343 .

Spasić, Snežana, Lugonja, Nikoleta, Laugier, Olga B., Oreščanin-Dušić, Zorana, Nikolić-Kokić, Aleksandra, Spasojević, Ivan, Vrvić, Miroslav, "Differences in direct pharmacological effects and antioxidative properties of mature breast milk and an infant formula" in 6th European Congress of Pharamcology “EPHAR 2012”, Granada, Spain, July 17-23 2012 (2012):351,
https://hdl.handle.net/21.15107/rcub_cer_5343 .

TY  - JOUR
AU  - Spasojević, Ivan
AU  - Obradović, Budimir
AU  - Spasić, Snežana
PY  - 2012
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1047
AB  - The present review is aimed at elucidating the neonatal 'sepsis redox cycle' - the cascade of inflammatory and redox events involved in the pathogenesis of sepsis in neonates. While adult and neonatal sepses share some common features, there are some substantial differences: higher mortality rates occur in adult sepsis and worse long-term effects are evident in neonatal sepsis survivors. Such epidemiological data may be explained by the lower ability of IL6 and IL8 to activate NF-kappa B-regulated transcription in neonatal sepsis in comparison to TNF-alpha, which is involved in the mechanisms of adult sepsis. The activation of NF-kappa B in neonatal sepsis is further promoted by hydrogen peroxide and results in mitochondrial dysfunction and energy failure as septic neonates experience decreased O-2 consumption as well as lower heat production and body temperature in comparison to healthy peers. In neonates, specific organs that are still under development are vulnerable to sepsis-provoked stress, which may lead to brain, lung, and heart injury, as well as vision and hearing impairments. In the light of the processes integrated here, it is clear that therapeutic approaches should also target specific steps in the neonatal 'sepsis redox cycle' in addition to the current therapeutic approach that is mainly focused on pathogen eradication.
PB  - Biomed Central Ltd, London
T2  - Critical Care
T1  - Bench-to-bedside review: Neonatal sepsis - redox processes in pathogenesis
VL  - 16
IS  - 3
DO  - 10.1186/cc11183
ER  - 

@article{
author = "Spasojević, Ivan and Obradović, Budimir and Spasić, Snežana",
year = "2012",
abstract = "The present review is aimed at elucidating the neonatal 'sepsis redox cycle' - the cascade of inflammatory and redox events involved in the pathogenesis of sepsis in neonates. While adult and neonatal sepses share some common features, there are some substantial differences: higher mortality rates occur in adult sepsis and worse long-term effects are evident in neonatal sepsis survivors. Such epidemiological data may be explained by the lower ability of IL6 and IL8 to activate NF-kappa B-regulated transcription in neonatal sepsis in comparison to TNF-alpha, which is involved in the mechanisms of adult sepsis. The activation of NF-kappa B in neonatal sepsis is further promoted by hydrogen peroxide and results in mitochondrial dysfunction and energy failure as septic neonates experience decreased O-2 consumption as well as lower heat production and body temperature in comparison to healthy peers. In neonates, specific organs that are still under development are vulnerable to sepsis-provoked stress, which may lead to brain, lung, and heart injury, as well as vision and hearing impairments. In the light of the processes integrated here, it is clear that therapeutic approaches should also target specific steps in the neonatal 'sepsis redox cycle' in addition to the current therapeutic approach that is mainly focused on pathogen eradication.",
publisher = "Biomed Central Ltd, London",
journal = "Critical Care",
title = "Bench-to-bedside review: Neonatal sepsis - redox processes in pathogenesis",
volume = "16",
number = "3",
doi = "10.1186/cc11183"
}

Spasojević, I., Obradović, B.,& Spasić, S.. (2012). Bench-to-bedside review: Neonatal sepsis - redox processes in pathogenesis. in Critical Care
Biomed Central Ltd, London., 16(3).
https://doi.org/10.1186/cc11183

Spasojević I, Obradović B, Spasić S. Bench-to-bedside review: Neonatal sepsis - redox processes in pathogenesis. in Critical Care. 2012;16(3).
doi:10.1186/cc11183 .

Spasojević, Ivan, Obradović, Budimir, Spasić, Snežana, "Bench-to-bedside review: Neonatal sepsis - redox processes in pathogenesis" in Critical Care, 16, no. 3 (2012),
https://doi.org/10.1186/cc11183 . .

TY  - JOUR
AU  - Bajic, Aleksandar
AU  - Zakrzewska, Joanna
AU  - Gođevac, Dejan
AU  - Anđus, Pavle
AU  - Jones, David R.
AU  - Spasić, Mihajlo
AU  - Spasojević, Ivan
PY  - 2011
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/781
AB  - The cytoprotective activity of F16BP has been documented in severe conditions such as convulsions, reperfusion injury, septic shock, diabetic complications, hypothermia-induced injury, UV-provoked skin damage and in other processes including apoptosis and excitotoxicity. F16BP shows very efficient cytoprotective activity in astroglial cells exposed to H2O2-provoked oxidative stress and during neuronal injury caused by hypoxic conditions. As most of the aforementioned processes involve iron activity-related conditions, we investigated the ferric and ferrous iron binding properties of F16BP under physiological conditions using P-31 NMR and EPR spectroscopy. Our results indicate that cytoprotective F16BP activity is predominantly based on ferrous iron sequestration. P-31 NMR spectroscopy of F16BP employing paramagnetic properties of iron clearly showed that F16BP forms stabile complexes with Fe2+ which was verified by EPR of another divalent cation-Mn2+. On the other hand, F16BP does not sequester ferric iron nor does it increase its redox activity as shown by P-31 NMR and EPR spin-trapping. Therefore, F16BP may be beneficial in neurodegenerative and other conditions that are characterised by ferric iron stores and deposits.
PB  - Elsevier Sci Ltd, Oxford
T2  - Carbohydrate Research
T1  - Relevance of the ability of fructose 1,6-bis(phosphate) to sequester ferrous but not ferric ions
VL  - 346
IS  - 3
SP  - 416
EP  - 420
DO  - 10.1016/j.carres.2010.12.008
ER  - 

@article{
author = "Bajic, Aleksandar and Zakrzewska, Joanna and Gođevac, Dejan and Anđus, Pavle and Jones, David R. and Spasić, Mihajlo and Spasojević, Ivan",
year = "2011",
abstract = "The cytoprotective activity of F16BP has been documented in severe conditions such as convulsions, reperfusion injury, septic shock, diabetic complications, hypothermia-induced injury, UV-provoked skin damage and in other processes including apoptosis and excitotoxicity. F16BP shows very efficient cytoprotective activity in astroglial cells exposed to H2O2-provoked oxidative stress and during neuronal injury caused by hypoxic conditions. As most of the aforementioned processes involve iron activity-related conditions, we investigated the ferric and ferrous iron binding properties of F16BP under physiological conditions using P-31 NMR and EPR spectroscopy. Our results indicate that cytoprotective F16BP activity is predominantly based on ferrous iron sequestration. P-31 NMR spectroscopy of F16BP employing paramagnetic properties of iron clearly showed that F16BP forms stabile complexes with Fe2+ which was verified by EPR of another divalent cation-Mn2+. On the other hand, F16BP does not sequester ferric iron nor does it increase its redox activity as shown by P-31 NMR and EPR spin-trapping. Therefore, F16BP may be beneficial in neurodegenerative and other conditions that are characterised by ferric iron stores and deposits.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Carbohydrate Research",
title = "Relevance of the ability of fructose 1,6-bis(phosphate) to sequester ferrous but not ferric ions",
volume = "346",
number = "3",
pages = "416-420",
doi = "10.1016/j.carres.2010.12.008"
}

Bajic, A., Zakrzewska, J., Gođevac, D., Anđus, P., Jones, D. R., Spasić, M.,& Spasojević, I.. (2011). Relevance of the ability of fructose 1,6-bis(phosphate) to sequester ferrous but not ferric ions. in Carbohydrate Research
Elsevier Sci Ltd, Oxford., 346(3), 416-420.
https://doi.org/10.1016/j.carres.2010.12.008

Bajic A, Zakrzewska J, Gođevac D, Anđus P, Jones DR, Spasić M, Spasojević I. Relevance of the ability of fructose 1,6-bis(phosphate) to sequester ferrous but not ferric ions. in Carbohydrate Research. 2011;346(3):416-420.
doi:10.1016/j.carres.2010.12.008 .

Bajic, Aleksandar, Zakrzewska, Joanna, Gođevac, Dejan, Anđus, Pavle, Jones, David R., Spasić, Mihajlo, Spasojević, Ivan, "Relevance of the ability of fructose 1,6-bis(phosphate) to sequester ferrous but not ferric ions" in Carbohydrate Research, 346, no. 3 (2011):416-420,
https://doi.org/10.1016/j.carres.2010.12.008 . .

TY  - JOUR
AU  - Andrades, Michael Everton
AU  - Morina, Arian
AU  - Spasić, Snežana
AU  - Spasojević, Ivan
PY  - 2011
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/822
AB  - The pathogenesis of sepsis and its progression to multiple organ dysfunction syndrome and septic shock have been the subject of investigations for nearly half a century. Controversies still exist with regard to understanding the molecular pathophysiology of sepsis in relation to the complex roles played by reactive oxygen species, nitric oxide, complements and cytokines. In the present review we categorise the key turning points in sepsis development and outline the most probable sequence of events leading to cellular dysfunction and organ failure under septic conditions. We have applied an integrative approach in order to fuse current state-of-the-art knowledge about redox processes involving hydrogen peroxide, nitric oxide, superoxide, peroxynitrite and hydroxyl radical, which lead to mitochondrial respiratory dysfunction. Finally, from this point of view, the potential of redox therapy targeting sepsis is discussed.
PB  - Biomed Central Ltd, London
T2  - Critical Care
T1  - Bench-to-bedside review: Sepsis - from the redox point of view
VL  - 15
IS  - 5
DO  - 10.1186/cc10334
ER  - 

@article{
author = "Andrades, Michael Everton and Morina, Arian and Spasić, Snežana and Spasojević, Ivan",
year = "2011",
abstract = "The pathogenesis of sepsis and its progression to multiple organ dysfunction syndrome and septic shock have been the subject of investigations for nearly half a century. Controversies still exist with regard to understanding the molecular pathophysiology of sepsis in relation to the complex roles played by reactive oxygen species, nitric oxide, complements and cytokines. In the present review we categorise the key turning points in sepsis development and outline the most probable sequence of events leading to cellular dysfunction and organ failure under septic conditions. We have applied an integrative approach in order to fuse current state-of-the-art knowledge about redox processes involving hydrogen peroxide, nitric oxide, superoxide, peroxynitrite and hydroxyl radical, which lead to mitochondrial respiratory dysfunction. Finally, from this point of view, the potential of redox therapy targeting sepsis is discussed.",
publisher = "Biomed Central Ltd, London",
journal = "Critical Care",
title = "Bench-to-bedside review: Sepsis - from the redox point of view",
volume = "15",
number = "5",
doi = "10.1186/cc10334"
}

Andrades, M. E., Morina, A., Spasić, S.,& Spasojević, I.. (2011). Bench-to-bedside review: Sepsis - from the redox point of view. in Critical Care
Biomed Central Ltd, London., 15(5).
https://doi.org/10.1186/cc10334

Andrades ME, Morina A, Spasić S, Spasojević I. Bench-to-bedside review: Sepsis - from the redox point of view. in Critical Care. 2011;15(5).
doi:10.1186/cc10334 .

Andrades, Michael Everton, Morina, Arian, Spasić, Snežana, Spasojević, Ivan, "Bench-to-bedside review: Sepsis - from the redox point of view" in Critical Care, 15, no. 5 (2011),
https://doi.org/10.1186/cc10334 . .