TY  - JOUR
AU  - Nisavic, Marija
AU  - Janjić, Goran
AU  - Hozic, Amela
AU  - Petkovic, Marijana
AU  - Milčić, Miloš
AU  - Vujčić, Zoran
AU  - Cindric, Mario
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2296
AB  - Binding of three ruthenium(ii) compounds of general formula mer-[Ru(L3)(N-N)X][Y] (where L3 = 4-chloro-2,2:6,2-terpyridine (Cl-tpy); N-N = 1,2-diaminoethane (en), 1,2-diaminocyclohexane (dach) or 2,2-bipyridine (bipy); X = Cl; Y = Cl) to human serum albumin (HSA) has been investigated by nano-LC/nano-ESI MS and docking studies. A bottom-up proteomics approach has been applied for the structural characterization of metallated proteins and the data were analyzed in both the positive and negative ion mode. The negative ion mode was achieved after the post-column addition of an isopropanol solution of formaldehyde that enabled sample ionization at micro-flow rates. The negative ion mode MS has been proved to be beneficial for the analysis of binding sites on ruthenated protein in terms of ion charge reduction and consequent simplification of target sequence identification based on isotopic differences between ruthenated and non-ruthenated peptides. Moreover, the negative ion mode ESI MS shows the advantage of singly charged ion formation and, unlike MALDI MS, it does not cause complete ligand fragmentation, merging the benefits of each method into a single experiment. Six target sequences were identified for the binding of en and dach compounds, and four sequences for the binding of bipy. All compounds have been found to bind histidine and one aspartate residue. Docking studies showed that the identified sequences are the constituents of five distinct binding sites for en and dach, or two sites for the bipy complex. The selection of binding sites seems to be dependent on the chelate ligand and the form of the complex prior or after hydrolysis of the leaving chloride ligand.
PB  - Royal Soc Chemistry, Cambridge
T2  - Metallomics
T1  - Positive and negative nano-electrospray mass spectrometry of ruthenated serum albumin supported by docking studies: an integrated approach towards defining metallodrug binding sites on proteins
VL  - 10
IS  - 4
SP  - 587
EP  - 594
DO  - 10.1039/c7mt00330g
ER  - 

@article{
author = "Nisavic, Marija and Janjić, Goran and Hozic, Amela and Petkovic, Marijana and Milčić, Miloš and Vujčić, Zoran and Cindric, Mario",
year = "2018",
abstract = "Binding of three ruthenium(ii) compounds of general formula mer-[Ru(L3)(N-N)X][Y] (where L3 = 4-chloro-2,2:6,2-terpyridine (Cl-tpy); N-N = 1,2-diaminoethane (en), 1,2-diaminocyclohexane (dach) or 2,2-bipyridine (bipy); X = Cl; Y = Cl) to human serum albumin (HSA) has been investigated by nano-LC/nano-ESI MS and docking studies. A bottom-up proteomics approach has been applied for the structural characterization of metallated proteins and the data were analyzed in both the positive and negative ion mode. The negative ion mode was achieved after the post-column addition of an isopropanol solution of formaldehyde that enabled sample ionization at micro-flow rates. The negative ion mode MS has been proved to be beneficial for the analysis of binding sites on ruthenated protein in terms of ion charge reduction and consequent simplification of target sequence identification based on isotopic differences between ruthenated and non-ruthenated peptides. Moreover, the negative ion mode ESI MS shows the advantage of singly charged ion formation and, unlike MALDI MS, it does not cause complete ligand fragmentation, merging the benefits of each method into a single experiment. Six target sequences were identified for the binding of en and dach compounds, and four sequences for the binding of bipy. All compounds have been found to bind histidine and one aspartate residue. Docking studies showed that the identified sequences are the constituents of five distinct binding sites for en and dach, or two sites for the bipy complex. The selection of binding sites seems to be dependent on the chelate ligand and the form of the complex prior or after hydrolysis of the leaving chloride ligand.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Metallomics",
title = "Positive and negative nano-electrospray mass spectrometry of ruthenated serum albumin supported by docking studies: an integrated approach towards defining metallodrug binding sites on proteins",
volume = "10",
number = "4",
pages = "587-594",
doi = "10.1039/c7mt00330g"
}

Nisavic, M., Janjić, G., Hozic, A., Petkovic, M., Milčić, M., Vujčić, Z.,& Cindric, M.. (2018). Positive and negative nano-electrospray mass spectrometry of ruthenated serum albumin supported by docking studies: an integrated approach towards defining metallodrug binding sites on proteins. in Metallomics
Royal Soc Chemistry, Cambridge., 10(4), 587-594.
https://doi.org/10.1039/c7mt00330g

Nisavic M, Janjić G, Hozic A, Petkovic M, Milčić M, Vujčić Z, Cindric M. Positive and negative nano-electrospray mass spectrometry of ruthenated serum albumin supported by docking studies: an integrated approach towards defining metallodrug binding sites on proteins. in Metallomics. 2018;10(4):587-594.
doi:10.1039/c7mt00330g .

Nisavic, Marija, Janjić, Goran, Hozic, Amela, Petkovic, Marijana, Milčić, Miloš, Vujčić, Zoran, Cindric, Mario, "Positive and negative nano-electrospray mass spectrometry of ruthenated serum albumin supported by docking studies: an integrated approach towards defining metallodrug binding sites on proteins" in Metallomics, 10, no. 4 (2018):587-594,
https://doi.org/10.1039/c7mt00330g . .

TY  - JOUR
AU  - Nisavic, Marija
AU  - Janjić, Goran
AU  - Hozic, Amela
AU  - Petkovic, Marijana
AU  - Milčić, Miloš
AU  - Vujčić, Zoran
AU  - Cindric, Mario
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3339
AB  - Binding of three ruthenium(ii) compounds of general formula mer-[Ru(L3)(N-N)X][Y] (where L3 = 4-chloro-2,2:6,2-terpyridine (Cl-tpy); N-N = 1,2-diaminoethane (en), 1,2-diaminocyclohexane (dach) or 2,2-bipyridine (bipy); X = Cl; Y = Cl) to human serum albumin (HSA) has been investigated by nano-LC/nano-ESI MS and docking studies. A bottom-up proteomics approach has been applied for the structural characterization of metallated proteins and the data were analyzed in both the positive and negative ion mode. The negative ion mode was achieved after the post-column addition of an isopropanol solution of formaldehyde that enabled sample ionization at micro-flow rates. The negative ion mode MS has been proved to be beneficial for the analysis of binding sites on ruthenated protein in terms of ion charge reduction and consequent simplification of target sequence identification based on isotopic differences between ruthenated and non-ruthenated peptides. Moreover, the negative ion mode ESI MS shows the advantage of singly charged ion formation and, unlike MALDI MS, it does not cause complete ligand fragmentation, merging the benefits of each method into a single experiment. Six target sequences were identified for the binding of en and dach compounds, and four sequences for the binding of bipy. All compounds have been found to bind histidine and one aspartate residue. Docking studies showed that the identified sequences are the constituents of five distinct binding sites for en and dach, or two sites for the bipy complex. The selection of binding sites seems to be dependent on the chelate ligand and the form of the complex prior or after hydrolysis of the leaving chloride ligand.
PB  - Royal Soc Chemistry, Cambridge
T2  - Metallomics
T1  - Positive and negative nano-electrospray mass spectrometry of ruthenated serum albumin supported by docking studies: an integrated approach towards defining metallodrug binding sites on proteins
VL  - 10
IS  - 4
SP  - 587
EP  - 594
DO  - 10.1039/c7mt00330g
ER  - 

@article{
author = "Nisavic, Marija and Janjić, Goran and Hozic, Amela and Petkovic, Marijana and Milčić, Miloš and Vujčić, Zoran and Cindric, Mario",
year = "2018",
abstract = "Binding of three ruthenium(ii) compounds of general formula mer-[Ru(L3)(N-N)X][Y] (where L3 = 4-chloro-2,2:6,2-terpyridine (Cl-tpy); N-N = 1,2-diaminoethane (en), 1,2-diaminocyclohexane (dach) or 2,2-bipyridine (bipy); X = Cl; Y = Cl) to human serum albumin (HSA) has been investigated by nano-LC/nano-ESI MS and docking studies. A bottom-up proteomics approach has been applied for the structural characterization of metallated proteins and the data were analyzed in both the positive and negative ion mode. The negative ion mode was achieved after the post-column addition of an isopropanol solution of formaldehyde that enabled sample ionization at micro-flow rates. The negative ion mode MS has been proved to be beneficial for the analysis of binding sites on ruthenated protein in terms of ion charge reduction and consequent simplification of target sequence identification based on isotopic differences between ruthenated and non-ruthenated peptides. Moreover, the negative ion mode ESI MS shows the advantage of singly charged ion formation and, unlike MALDI MS, it does not cause complete ligand fragmentation, merging the benefits of each method into a single experiment. Six target sequences were identified for the binding of en and dach compounds, and four sequences for the binding of bipy. All compounds have been found to bind histidine and one aspartate residue. Docking studies showed that the identified sequences are the constituents of five distinct binding sites for en and dach, or two sites for the bipy complex. The selection of binding sites seems to be dependent on the chelate ligand and the form of the complex prior or after hydrolysis of the leaving chloride ligand.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Metallomics",
title = "Positive and negative nano-electrospray mass spectrometry of ruthenated serum albumin supported by docking studies: an integrated approach towards defining metallodrug binding sites on proteins",
volume = "10",
number = "4",
pages = "587-594",
doi = "10.1039/c7mt00330g"
}

Nisavic, M., Janjić, G., Hozic, A., Petkovic, M., Milčić, M., Vujčić, Z.,& Cindric, M.. (2018). Positive and negative nano-electrospray mass spectrometry of ruthenated serum albumin supported by docking studies: an integrated approach towards defining metallodrug binding sites on proteins. in Metallomics
Royal Soc Chemistry, Cambridge., 10(4), 587-594.
https://doi.org/10.1039/c7mt00330g

Nisavic M, Janjić G, Hozic A, Petkovic M, Milčić M, Vujčić Z, Cindric M. Positive and negative nano-electrospray mass spectrometry of ruthenated serum albumin supported by docking studies: an integrated approach towards defining metallodrug binding sites on proteins. in Metallomics. 2018;10(4):587-594.
doi:10.1039/c7mt00330g .

Nisavic, Marija, Janjić, Goran, Hozic, Amela, Petkovic, Marijana, Milčić, Miloš, Vujčić, Zoran, Cindric, Mario, "Positive and negative nano-electrospray mass spectrometry of ruthenated serum albumin supported by docking studies: an integrated approach towards defining metallodrug binding sites on proteins" in Metallomics, 10, no. 4 (2018):587-594,
https://doi.org/10.1039/c7mt00330g . .

TY  - JOUR
AU  - Dolic, Maja B.
AU  - Rajakovic-Ognjanovic, Vladana N.
AU  - Štrbac, Svetlana
AU  - Dimitrijević, Suzana I.
AU  - Mitrić, Miodrag
AU  - Onjia, Antonije E.
AU  - Rajaković, Ljubinka V.
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3030
AB  - The objective of this study was to investigate the modification of materials used in wastewater treatment for possible antimicrobial application(s). Granulated activated carbon (GAC) and natural clinoptilolite (CLI) were activated using Cu2+-and Zn2+-ions and the disinfection ability of the resulting materials was tested. Studies of the sorption and desorption kinetics were performed in order to determine and clarify the antimicrobial activity of the metal-activated sorbents. The exact sorption capacities of the selected sorbents, GAC and CLI, activated through use of Cu2+-ions, were 15.90 and 3.60 mg/g, respectively, while for the materials activated by Zn2+-ions, the corresponding capacities were 14.00 and 4.72 mg/g,. The desorption rates were 2 and 3 orders of magnitude lower than their sorption efficacy for the Cu2+-, and Zn2+-activated sorbents, respectively. The intermediate sorption capacity and low desorption rate indicated that the overall antimicrobial activity of the metal-modified sorbents was a result of metal ions immobilized onto surface sites. The effect of antimicrobial activity of free ions desorbed from the metal-activated surface may thus be disregarded. The antimicrobial activities of Cu/GAC, Zn/GAC, Cu/CLI and Zn/ CLI were also tested against Escherichia coli, Staphylococcus aureus, and Candida albicans. After 15 min exposure, the highest levels of cell inactivation were obtained through the Cu/CLI and the Cu/GAC against E. coli, 100.0 and 98.24%, respectively. However, for S. aureus and yeast cell inactivation, all Cu2+-and Zn2+-activated sorbents proved to be unsatisfactory. A characterization of the sorbents was performed by Xray diffraction (XRD), X-ray photo electron spectroscopy (XPS), and field emission scanning electron microscopy (FE-SEM). A concentration of the adsorbed and released ions was determined by inductively coupled plasma-optical emission spectroscopy (ICP-OES) and mass spectrometry (ICP-MS). The results showed that the antimicrobial performance of the activated sorbents depended on the surface characteristics of the material, which itself designates the distribution and the bioavailability of the activating agent.
PB  - Elsevier
T2  - New Biotechnology
T1  - Natural sorbents modified by divalent Cu2+- and Zn2+- ions and their corresponding antimicrobial activity
VL  - 39
SP  - 150
EP  - 159
DO  - 10.1016/j.nbt.2017.03.001
ER  - 

@article{
author = "Dolic, Maja B. and Rajakovic-Ognjanovic, Vladana N. and Štrbac, Svetlana and Dimitrijević, Suzana I. and Mitrić, Miodrag and Onjia, Antonije E. and Rajaković, Ljubinka V.",
year = "2017",
abstract = "The objective of this study was to investigate the modification of materials used in wastewater treatment for possible antimicrobial application(s). Granulated activated carbon (GAC) and natural clinoptilolite (CLI) were activated using Cu2+-and Zn2+-ions and the disinfection ability of the resulting materials was tested. Studies of the sorption and desorption kinetics were performed in order to determine and clarify the antimicrobial activity of the metal-activated sorbents. The exact sorption capacities of the selected sorbents, GAC and CLI, activated through use of Cu2+-ions, were 15.90 and 3.60 mg/g, respectively, while for the materials activated by Zn2+-ions, the corresponding capacities were 14.00 and 4.72 mg/g,. The desorption rates were 2 and 3 orders of magnitude lower than their sorption efficacy for the Cu2+-, and Zn2+-activated sorbents, respectively. The intermediate sorption capacity and low desorption rate indicated that the overall antimicrobial activity of the metal-modified sorbents was a result of metal ions immobilized onto surface sites. The effect of antimicrobial activity of free ions desorbed from the metal-activated surface may thus be disregarded. The antimicrobial activities of Cu/GAC, Zn/GAC, Cu/CLI and Zn/ CLI were also tested against Escherichia coli, Staphylococcus aureus, and Candida albicans. After 15 min exposure, the highest levels of cell inactivation were obtained through the Cu/CLI and the Cu/GAC against E. coli, 100.0 and 98.24%, respectively. However, for S. aureus and yeast cell inactivation, all Cu2+-and Zn2+-activated sorbents proved to be unsatisfactory. A characterization of the sorbents was performed by Xray diffraction (XRD), X-ray photo electron spectroscopy (XPS), and field emission scanning electron microscopy (FE-SEM). A concentration of the adsorbed and released ions was determined by inductively coupled plasma-optical emission spectroscopy (ICP-OES) and mass spectrometry (ICP-MS). The results showed that the antimicrobial performance of the activated sorbents depended on the surface characteristics of the material, which itself designates the distribution and the bioavailability of the activating agent.",
publisher = "Elsevier",
journal = "New Biotechnology",
title = "Natural sorbents modified by divalent Cu2+- and Zn2+- ions and their corresponding antimicrobial activity",
volume = "39",
pages = "150-159",
doi = "10.1016/j.nbt.2017.03.001"
}

Dolic, M. B., Rajakovic-Ognjanovic, V. N., Štrbac, S., Dimitrijević, S. I., Mitrić, M., Onjia, A. E.,& Rajaković, L. V.. (2017). Natural sorbents modified by divalent Cu2+- and Zn2+- ions and their corresponding antimicrobial activity. in New Biotechnology
Elsevier., 39, 150-159.
https://doi.org/10.1016/j.nbt.2017.03.001

Dolic MB, Rajakovic-Ognjanovic VN, Štrbac S, Dimitrijević SI, Mitrić M, Onjia AE, Rajaković LV. Natural sorbents modified by divalent Cu2+- and Zn2+- ions and their corresponding antimicrobial activity. in New Biotechnology. 2017;39:150-159.
doi:10.1016/j.nbt.2017.03.001 .

Dolic, Maja B., Rajakovic-Ognjanovic, Vladana N., Štrbac, Svetlana, Dimitrijević, Suzana I., Mitrić, Miodrag, Onjia, Antonije E., Rajaković, Ljubinka V., "Natural sorbents modified by divalent Cu2+- and Zn2+- ions and their corresponding antimicrobial activity" in New Biotechnology, 39 (2017):150-159,
https://doi.org/10.1016/j.nbt.2017.03.001 . .

TY  - JOUR
AU  - Dolic, Maja B.
AU  - Rajakovic-Ognjanovic, Vladana N.
AU  - Štrbac, Svetlana
AU  - Dimitrijević, Suzana I.
AU  - Mitrić, Miodrag
AU  - Onjia, Antonije E.
AU  - Rajaković, Ljubinka V.
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2158
AB  - The objective of this study was to investigate the modification of materials used in wastewater treatment for possible antimicrobial application(s). Granulated activated carbon (GAC) and natural clinoptilolite (CLI) were activated using Cu2+-and Zn2+-ions and the disinfection ability of the resulting materials was tested. Studies of the sorption and desorption kinetics were performed in order to determine and clarify the antimicrobial activity of the metal-activated sorbents. The exact sorption capacities of the selected sorbents, GAC and CLI, activated through use of Cu2+-ions, were 15.90 and 3.60 mg/g, respectively, while for the materials activated by Zn2+-ions, the corresponding capacities were 14.00 and 4.72 mg/g,. The desorption rates were 2 and 3 orders of magnitude lower than their sorption efficacy for the Cu2+-, and Zn2+-activated sorbents, respectively. The intermediate sorption capacity and low desorption rate indicated that the overall antimicrobial activity of the metal-modified sorbents was a result of metal ions immobilized onto surface sites. The effect of antimicrobial activity of free ions desorbed from the metal-activated surface may thus be disregarded. The antimicrobial activities of Cu/GAC, Zn/GAC, Cu/CLI and Zn/ CLI were also tested against Escherichia coli, Staphylococcus aureus, and Candida albicans. After 15 min exposure, the highest levels of cell inactivation were obtained through the Cu/CLI and the Cu/GAC against E. coli, 100.0 and 98.24%, respectively. However, for S. aureus and yeast cell inactivation, all Cu2+-and Zn2+-activated sorbents proved to be unsatisfactory. A characterization of the sorbents was performed by Xray diffraction (XRD), X-ray photo electron spectroscopy (XPS), and field emission scanning electron microscopy (FE-SEM). A concentration of the adsorbed and released ions was determined by inductively coupled plasma-optical emission spectroscopy (ICP-OES) and mass spectrometry (ICP-MS). The results showed that the antimicrobial performance of the activated sorbents depended on the surface characteristics of the material, which itself designates the distribution and the bioavailability of the activating agent.
PB  - Elsevier
T2  - New Biotechnology
T1  - Natural sorbents modified by divalent Cu2+- and Zn2+- ions and their corresponding antimicrobial activity
VL  - 39
SP  - 150
EP  - 159
DO  - 10.1016/j.nbt.2017.03.001
ER  - 

@article{
author = "Dolic, Maja B. and Rajakovic-Ognjanovic, Vladana N. and Štrbac, Svetlana and Dimitrijević, Suzana I. and Mitrić, Miodrag and Onjia, Antonije E. and Rajaković, Ljubinka V.",
year = "2017",
abstract = "The objective of this study was to investigate the modification of materials used in wastewater treatment for possible antimicrobial application(s). Granulated activated carbon (GAC) and natural clinoptilolite (CLI) were activated using Cu2+-and Zn2+-ions and the disinfection ability of the resulting materials was tested. Studies of the sorption and desorption kinetics were performed in order to determine and clarify the antimicrobial activity of the metal-activated sorbents. The exact sorption capacities of the selected sorbents, GAC and CLI, activated through use of Cu2+-ions, were 15.90 and 3.60 mg/g, respectively, while for the materials activated by Zn2+-ions, the corresponding capacities were 14.00 and 4.72 mg/g,. The desorption rates were 2 and 3 orders of magnitude lower than their sorption efficacy for the Cu2+-, and Zn2+-activated sorbents, respectively. The intermediate sorption capacity and low desorption rate indicated that the overall antimicrobial activity of the metal-modified sorbents was a result of metal ions immobilized onto surface sites. The effect of antimicrobial activity of free ions desorbed from the metal-activated surface may thus be disregarded. The antimicrobial activities of Cu/GAC, Zn/GAC, Cu/CLI and Zn/ CLI were also tested against Escherichia coli, Staphylococcus aureus, and Candida albicans. After 15 min exposure, the highest levels of cell inactivation were obtained through the Cu/CLI and the Cu/GAC against E. coli, 100.0 and 98.24%, respectively. However, for S. aureus and yeast cell inactivation, all Cu2+-and Zn2+-activated sorbents proved to be unsatisfactory. A characterization of the sorbents was performed by Xray diffraction (XRD), X-ray photo electron spectroscopy (XPS), and field emission scanning electron microscopy (FE-SEM). A concentration of the adsorbed and released ions was determined by inductively coupled plasma-optical emission spectroscopy (ICP-OES) and mass spectrometry (ICP-MS). The results showed that the antimicrobial performance of the activated sorbents depended on the surface characteristics of the material, which itself designates the distribution and the bioavailability of the activating agent.",
publisher = "Elsevier",
journal = "New Biotechnology",
title = "Natural sorbents modified by divalent Cu2+- and Zn2+- ions and their corresponding antimicrobial activity",
volume = "39",
pages = "150-159",
doi = "10.1016/j.nbt.2017.03.001"
}

Dolic, M. B., Rajakovic-Ognjanovic, V. N., Štrbac, S., Dimitrijević, S. I., Mitrić, M., Onjia, A. E.,& Rajaković, L. V.. (2017). Natural sorbents modified by divalent Cu2+- and Zn2+- ions and their corresponding antimicrobial activity. in New Biotechnology
Elsevier., 39, 150-159.
https://doi.org/10.1016/j.nbt.2017.03.001

Dolic MB, Rajakovic-Ognjanovic VN, Štrbac S, Dimitrijević SI, Mitrić M, Onjia AE, Rajaković LV. Natural sorbents modified by divalent Cu2+- and Zn2+- ions and their corresponding antimicrobial activity. in New Biotechnology. 2017;39:150-159.
doi:10.1016/j.nbt.2017.03.001 .

Dolic, Maja B., Rajakovic-Ognjanovic, Vladana N., Štrbac, Svetlana, Dimitrijević, Suzana I., Mitrić, Miodrag, Onjia, Antonije E., Rajaković, Ljubinka V., "Natural sorbents modified by divalent Cu2+- and Zn2+- ions and their corresponding antimicrobial activity" in New Biotechnology, 39 (2017):150-159,
https://doi.org/10.1016/j.nbt.2017.03.001 . .