TY  - JOUR
AU  - Tasić, Ljubica
AU  - Avramović, Nataša
AU  - Quintero, Melissa
AU  - Stanišić, Danijela
AU  - Martins, Lucas
AU  - Brena da Costa, Tássia
AU  - Jadranin, Milka
AU  - Accioly de Souza, Maria Theresa
AU  - Faria, Paulo
AU  - de Camargo, Beatriz
AU  - de Sá Pereira, Bruna Maria
AU  - Maschietto, Mariana
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5323
AB  - Pediatric cancer NMR-metabonomics might be a powerful tool to discover modified
biochemical pathways in tumor development, improve cancer diagnosis, and, consequently, treatment.
Wilms tumor (WT) is the most common kidney tumor in young children whose genetic and epigenetic
abnormalities lead to cell metabolism alterations, but, so far, investigation of metabolic pathways
in WT is scarce. We aimed to explore the high-resolution magic-angle spinning nuclear magnetic
resonance (HR-MAS NMR) metabonomics of WT and normal kidney (NK) samples. For this study,
14 WT and 7 NK tissue samples were obtained from the same patients and analyzed. One-dimensional
and two-dimensional HR-MAS NMR spectra were processed, and the one-dimensional NMR data
were analyzed using chemometrics. Chemometrics enabled us to elucidate the most significant
differences between the tumor and normal tissues and to discover intrinsic metabolite alterations in
WT. The metabolic differences in WT tissues were revealed by a validated PLS-DA applied on HR MAS T2
-edited 1H-NMR and were assigned to 16 metabolites, such as lipids, glucose, and branched chain amino acids (BCAAs), among others. The WT compared to NK samples showed 13 metabolites
with increased concentrations and 3 metabolites with decreased concentrations. The relative BCAA
concentrations were decreased in the WT while lipids, lactate, and glutamine/glutamate showed
increased levels. Sixteen tissue metabolites distinguish the analyzed WT samples and point to altered
glycolysis, glutaminolysis, TCA cycle, and lipid and BCAA metabolism in WT. Significant variation
in the concentrations of metabolites, such as glutamine/glutamate, lipids, lactate, and BCAAs, was
observed in WT and opened up a perspective for their further study and clinical validation.
PB  - MDPI
T2  - Diagnostic
T1  - A Metabonomic View on Wilms Tumor by High-Resolution Magic-Angle Spinning Nuclear Magnetic Resonance Spectroscopy
VL  - 12
IS  - 1
SP  - 2
EP  - 10
DO  - 10.3390/diagnostics12010157
ER  - 

@article{
author = "Tasić, Ljubica and Avramović, Nataša and Quintero, Melissa and Stanišić, Danijela and Martins, Lucas and Brena da Costa, Tássia and Jadranin, Milka and Accioly de Souza, Maria Theresa and Faria, Paulo and de Camargo, Beatriz and de Sá Pereira, Bruna Maria and Maschietto, Mariana",
year = "2022",
abstract = "Pediatric cancer NMR-metabonomics might be a powerful tool to discover modified
biochemical pathways in tumor development, improve cancer diagnosis, and, consequently, treatment.
Wilms tumor (WT) is the most common kidney tumor in young children whose genetic and epigenetic
abnormalities lead to cell metabolism alterations, but, so far, investigation of metabolic pathways
in WT is scarce. We aimed to explore the high-resolution magic-angle spinning nuclear magnetic
resonance (HR-MAS NMR) metabonomics of WT and normal kidney (NK) samples. For this study,
14 WT and 7 NK tissue samples were obtained from the same patients and analyzed. One-dimensional
and two-dimensional HR-MAS NMR spectra were processed, and the one-dimensional NMR data
were analyzed using chemometrics. Chemometrics enabled us to elucidate the most significant
differences between the tumor and normal tissues and to discover intrinsic metabolite alterations in
WT. The metabolic differences in WT tissues were revealed by a validated PLS-DA applied on HR MAS T2
-edited 1H-NMR and were assigned to 16 metabolites, such as lipids, glucose, and branched chain amino acids (BCAAs), among others. The WT compared to NK samples showed 13 metabolites
with increased concentrations and 3 metabolites with decreased concentrations. The relative BCAA
concentrations were decreased in the WT while lipids, lactate, and glutamine/glutamate showed
increased levels. Sixteen tissue metabolites distinguish the analyzed WT samples and point to altered
glycolysis, glutaminolysis, TCA cycle, and lipid and BCAA metabolism in WT. Significant variation
in the concentrations of metabolites, such as glutamine/glutamate, lipids, lactate, and BCAAs, was
observed in WT and opened up a perspective for their further study and clinical validation.",
publisher = "MDPI",
journal = "Diagnostic",
title = "A Metabonomic View on Wilms Tumor by High-Resolution Magic-Angle Spinning Nuclear Magnetic Resonance Spectroscopy",
volume = "12",
number = "1",
pages = "2-10",
doi = "10.3390/diagnostics12010157"
}

Tasić, L., Avramović, N., Quintero, M., Stanišić, D., Martins, L., Brena da Costa, T., Jadranin, M., Accioly de Souza, M. T., Faria, P., de Camargo, B., de Sá Pereira, B. M.,& Maschietto, M.. (2022). A Metabonomic View on Wilms Tumor by High-Resolution Magic-Angle Spinning Nuclear Magnetic Resonance Spectroscopy. in Diagnostic
MDPI., 12(1), 2-10.
https://doi.org/10.3390/diagnostics12010157

Tasić L, Avramović N, Quintero M, Stanišić D, Martins L, Brena da Costa T, Jadranin M, Accioly de Souza MT, Faria P, de Camargo B, de Sá Pereira BM, Maschietto M. A Metabonomic View on Wilms Tumor by High-Resolution Magic-Angle Spinning Nuclear Magnetic Resonance Spectroscopy. in Diagnostic. 2022;12(1):2-10.
doi:10.3390/diagnostics12010157 .

Tasić, Ljubica, Avramović, Nataša, Quintero, Melissa, Stanišić, Danijela, Martins, Lucas, Brena da Costa, Tássia, Jadranin, Milka, Accioly de Souza, Maria Theresa, Faria, Paulo, de Camargo, Beatriz, de Sá Pereira, Bruna Maria, Maschietto, Mariana, "A Metabonomic View on Wilms Tumor by High-Resolution Magic-Angle Spinning Nuclear Magnetic Resonance Spectroscopy" in Diagnostic, 12, no. 1 (2022):2-10,
https://doi.org/10.3390/diagnostics12010157 . .