TY  - JOUR
AU  - Nikolić, Biljana
AU  - Todosijević, Marina
AU  - Đorđević, Iris
AU  - Stanković, Jovana
AU  - Mitić, Zorica S.
AU  - Marin, Petar D.
AU  - Tešević, Vele
PY  - 2020
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3953
AB  - In leaf cuticular wax of Pinus pinaster, content of nonacosan-10-ol is high (77.1% on average). n-Alkanes ranged from C18 to C35
with the most dominant C29 (24.8%). The carbon preference index (CPItotal) ranged from 3.1 to 5.6 (4.0 on average), while the
average chain length (ACLtotal) ranged from 14.0 to 17.0 (14.8 on average). Long-chain n-alkanes (n-C25-35) strongly dominated
(80.1%) over middle-chain (n-C21-24 = 18.9%) and short-chain (n-C18-20 = 0.9%) n-alkanes.
PB  - SAGE Publications Inc.
T2  - Natural Product Communications
T1  - Nonacosan-10-ol and n-Alkanes in Leaves of Pinus pinaster
VL  - 15
IS  - 5
SP  - 1
EP  - 4
DO  - 10.1177/1934578X20926073
ER  - 

@article{
author = "Nikolić, Biljana and Todosijević, Marina and Đorđević, Iris and Stanković, Jovana and Mitić, Zorica S. and Marin, Petar D. and Tešević, Vele",
year = "2020",
abstract = "In leaf cuticular wax of Pinus pinaster, content of nonacosan-10-ol is high (77.1% on average). n-Alkanes ranged from C18 to C35
with the most dominant C29 (24.8%). The carbon preference index (CPItotal) ranged from 3.1 to 5.6 (4.0 on average), while the
average chain length (ACLtotal) ranged from 14.0 to 17.0 (14.8 on average). Long-chain n-alkanes (n-C25-35) strongly dominated
(80.1%) over middle-chain (n-C21-24 = 18.9%) and short-chain (n-C18-20 = 0.9%) n-alkanes.",
publisher = "SAGE Publications Inc.",
journal = "Natural Product Communications",
title = "Nonacosan-10-ol and n-Alkanes in Leaves of Pinus pinaster",
volume = "15",
number = "5",
pages = "1-4",
doi = "10.1177/1934578X20926073"
}

Nikolić, B., Todosijević, M., Đorđević, I., Stanković, J., Mitić, Z. S., Marin, P. D.,& Tešević, V.. (2020). Nonacosan-10-ol and n-Alkanes in Leaves of Pinus pinaster. in Natural Product Communications
SAGE Publications Inc.., 15(5), 1-4.
https://doi.org/10.1177/1934578X20926073

Nikolić B, Todosijević M, Đorđević I, Stanković J, Mitić ZS, Marin PD, Tešević V. Nonacosan-10-ol and n-Alkanes in Leaves of Pinus pinaster. in Natural Product Communications. 2020;15(5):1-4.
doi:10.1177/1934578X20926073 .

Nikolić, Biljana, Todosijević, Marina, Đorđević, Iris, Stanković, Jovana, Mitić, Zorica S., Marin, Petar D., Tešević, Vele, "Nonacosan-10-ol and n-Alkanes in Leaves of Pinus pinaster" in Natural Product Communications, 15, no. 5 (2020):1-4,
https://doi.org/10.1177/1934578X20926073 . .

TY  - JOUR
AU  - Tovilović, Gordana
AU  - Zogovic, Nevena
AU  - Šoškić, Vukić
AU  - Schrattenholz, Andre
AU  - Kostić Rajačić, Slađana
AU  - Misirkić-Marjanović, Maja
AU  - Janjetovic, Kristina
AU  - Vucicevic, Ljubica
AU  - Arsikin, Katarina
AU  - Harhaji-Trajković, Ljubica
AU  - Trajković, Vladimir
PY  - 2013
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1201
AB  - We investigated the ability of 19 recently synthesized arylpiperazine compounds to protect human SH-SY5Y neuroblastoma cells from the neurotoxin 6-hydroxydopamine (6-OHDA). The compound with the most potent neuroprotective action was N-{3-[2-(4-phenyl-piperazin-1-yl)-ethyl]-phenyl}-picolinamide (6b), which reduced 6-OHDA-induced apoptotic death through stabilization of mitochondrial membrane and subsequent prevention of superoxide production, caspase activation and DNA fragmentation. 6-OHDA-triggered autophagic response was also reduced by 6b, which prevented inactivation of the main autophagy repressor mTOR, upregulation of proautophagic beclin-1, conversion of microtubule-associated protein 1 light chain 3 (LC3)-I to autophagosome-associAed LC3-II, as well as intracytoplasmic acidification induced by 6-OHDA. The inhibition of autophagy using LC3 beta gene silencing or pharmacological autophagy blockers 3-methyladenine or bafilomycin A1, mimicked the cytoprotective effect of 6b. While the treatment with 6b had no effect on the phosphorylation of proapoptotic MAP kinases ERR and JNK, it markedly increased the phosphorylation of the prosurvival kinase Akt in 6-OHDA-treated cells. Akt inhibitor DEBC or RNA interference-mediated Akt silencing reduced the ability of 6b to block 6-0HDA-triggered apoptotic and autophagic responses, thus confirming their dependency on Akt activation. The cytoprotective effect of 6b was also observed in 6-OHDA-treated neuronal PC12 cells, but not in SH-SY5Y or PC12 cells exposed to 1-methyl-4-phenylpyridinium, indicating that the observed neuroprotection was dependent on the cytotoxic stimulus. Because of the ability to prevent 6-OHDA induced apoptotic/autophagic cell death through activation of Akt, the investigated arylpiperazines could be potential candidates for treatment of neurodegenerative diseases.
PB  - Oxford : Pergamon-Elsevier Science Ltd
T2  - Neuropharmacology
T1  - Arylpiperazine-mediated activation of Akt protects SH-SY5Y neuroblastoma cells from 6-hydroxydopamine-induced apoptotic and autophagic death
VL  - 72
SP  - 224
EP  - 235
DO  - 10.1016/j.neuropharm.2013.04.037
ER  - 

@article{
author = "Tovilović, Gordana and Zogovic, Nevena and Šoškić, Vukić and Schrattenholz, Andre and Kostić Rajačić, Slađana and Misirkić-Marjanović, Maja and Janjetovic, Kristina and Vucicevic, Ljubica and Arsikin, Katarina and Harhaji-Trajković, Ljubica and Trajković, Vladimir",
year = "2013",
abstract = "We investigated the ability of 19 recently synthesized arylpiperazine compounds to protect human SH-SY5Y neuroblastoma cells from the neurotoxin 6-hydroxydopamine (6-OHDA). The compound with the most potent neuroprotective action was N-{3-[2-(4-phenyl-piperazin-1-yl)-ethyl]-phenyl}-picolinamide (6b), which reduced 6-OHDA-induced apoptotic death through stabilization of mitochondrial membrane and subsequent prevention of superoxide production, caspase activation and DNA fragmentation. 6-OHDA-triggered autophagic response was also reduced by 6b, which prevented inactivation of the main autophagy repressor mTOR, upregulation of proautophagic beclin-1, conversion of microtubule-associated protein 1 light chain 3 (LC3)-I to autophagosome-associAed LC3-II, as well as intracytoplasmic acidification induced by 6-OHDA. The inhibition of autophagy using LC3 beta gene silencing or pharmacological autophagy blockers 3-methyladenine or bafilomycin A1, mimicked the cytoprotective effect of 6b. While the treatment with 6b had no effect on the phosphorylation of proapoptotic MAP kinases ERR and JNK, it markedly increased the phosphorylation of the prosurvival kinase Akt in 6-OHDA-treated cells. Akt inhibitor DEBC or RNA interference-mediated Akt silencing reduced the ability of 6b to block 6-0HDA-triggered apoptotic and autophagic responses, thus confirming their dependency on Akt activation. The cytoprotective effect of 6b was also observed in 6-OHDA-treated neuronal PC12 cells, but not in SH-SY5Y or PC12 cells exposed to 1-methyl-4-phenylpyridinium, indicating that the observed neuroprotection was dependent on the cytotoxic stimulus. Because of the ability to prevent 6-OHDA induced apoptotic/autophagic cell death through activation of Akt, the investigated arylpiperazines could be potential candidates for treatment of neurodegenerative diseases.",
publisher = "Oxford : Pergamon-Elsevier Science Ltd",
journal = "Neuropharmacology",
title = "Arylpiperazine-mediated activation of Akt protects SH-SY5Y neuroblastoma cells from 6-hydroxydopamine-induced apoptotic and autophagic death",
volume = "72",
pages = "224-235",
doi = "10.1016/j.neuropharm.2013.04.037"
}

Tovilović, G., Zogovic, N., Šoškić, V., Schrattenholz, A., Kostić Rajačić, S., Misirkić-Marjanović, M., Janjetovic, K., Vucicevic, L., Arsikin, K., Harhaji-Trajković, L.,& Trajković, V.. (2013). Arylpiperazine-mediated activation of Akt protects SH-SY5Y neuroblastoma cells from 6-hydroxydopamine-induced apoptotic and autophagic death. in Neuropharmacology
Oxford : Pergamon-Elsevier Science Ltd., 72, 224-235.
https://doi.org/10.1016/j.neuropharm.2013.04.037

Tovilović G, Zogovic N, Šoškić V, Schrattenholz A, Kostić Rajačić S, Misirkić-Marjanović M, Janjetovic K, Vucicevic L, Arsikin K, Harhaji-Trajković L, Trajković V. Arylpiperazine-mediated activation of Akt protects SH-SY5Y neuroblastoma cells from 6-hydroxydopamine-induced apoptotic and autophagic death. in Neuropharmacology. 2013;72:224-235.
doi:10.1016/j.neuropharm.2013.04.037 .

Tovilović, Gordana, Zogovic, Nevena, Šoškić, Vukić, Schrattenholz, Andre, Kostić Rajačić, Slađana, Misirkić-Marjanović, Maja, Janjetovic, Kristina, Vucicevic, Ljubica, Arsikin, Katarina, Harhaji-Trajković, Ljubica, Trajković, Vladimir, "Arylpiperazine-mediated activation of Akt protects SH-SY5Y neuroblastoma cells from 6-hydroxydopamine-induced apoptotic and autophagic death" in Neuropharmacology, 72 (2013):224-235,
https://doi.org/10.1016/j.neuropharm.2013.04.037 . .