@conference{
author = "Krizmanić, Jelena and Stupar, Miloš and Ljaljević Grbić, Milica and Dimitrijević, Jelica and Vidaković, Danijela and Stamenković, Srđan and Krizmanić, Imre and Breka, Katarina",
year = "2019",
abstract = "Cutaneous microbiota of amphibians is considered
to be an indicator of the health status of examined
individuals, as well as of environmental factors. In
that sense the skin of the captured individuals
belonging to Pelophylax esculenta complex on the
locality Stevanove ravnice within the Special Nature
Reserve “Deliblato Sand” (Serbia) was screened for
the presence of biofilm-forming cyanobacteria,
algae and fungi dwelling directly on frogs’ stratum
corneum. Biofilm was sampled using non-aggressive
“adhesive tape method” and their constituents were
analyzed and characterized microscopically (Carl
Zeiss AxioImager.M1 with AxioVision 4.9 software).
We detected high diversity and abundance
of diatom taxa overall, particularly belonging to
genera Navicula, Gomphonema, Geissleria,
Placoneis and Nitzschia. Cyanobacteria were less
present and with low abundance. Among them
chroococcal genera Gloeocapsopsis, Pseudocapsa
and Aphanocapsa were recorded. Outside of diatoms
and cyanobacteria, genus Chlorella was the
only present photoautotrophic biofilm constituent.
We consider highly mucous green frogs skin as
a favorable environment for the survival of diatoms
and cyanobacteria. Light microscopy analyses of
biofilm fragments revealed the presence of variety
of fungal structures. Documented coenocytic mycelium
with zoosporangium, originated presumably
from oomycetes, as well as septate melanized
hyphae of dematiaceous fungi, suggested colonization
of frog skin from both types of environments
present, water and surrounding vegetation.
A variety of fungal spores (fragmospores, amerospores,
dictyospores, didymospores), and in few
cases spore germination were recorded on frog
skin. Detected biofilm composition was not determinate
by species and gender-specific.",
publisher = "Taylor & Francis",
journal = "European Journal of Phycology (Posters)",
title = "Green frogs skin harbours microbiota treasure",
volume = "54",
number = "sup1",
pages = "156-156",
doi = "10.1080/09670262.2019.1626628"
}