TY  - JOUR
AU  - Medić, Ana
AU  - Huttmann, Nico
AU  - Lješević, Marija
AU  - Risha, Yousef
AU  - Berezovski, Maxim
AU  - Minić, Zoran
AU  - Karadžić, Ivanka
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5548
AB  - Polyextremophilic, hydrocarbonoclastic Pseudomonas aeruginosa san ai can survive under extreme environmental challenges in the presence of a variety of pollutants such as organic solvents and hydrocarbons, particularly aromatics, heavy metals, and high pH. To date, the metabolic plasticity of the extremophilic P. aeruginosa, has not been sufficiently studied in regard to the effect of changing carbon sources. Therefore, the present study explores the carbon metabolic pathways of polyextremophilic P. aeruginosa san ai grown on sodium benzoate versus glucose and its potential for aromatic degradation. P. aeruginosa san ai removed/metabolised early 430 mg/L of benzoate for 48 h, demonstrating a high capacity for aromatic degradation. Comparative functional proteomics, targeted metabolomics and genomics analytical approaches were employed to study the carbon metabolism of the P. aeruginosa san ai. Functional proteomic study of selected enzymes participating in the β-ketoadipate and the Entner-Doudoroff pathways revealed a metabolic reconfiguration induced by benzoate compared to glucose. Metabolome analysis implied the existence of both catechol and protocatechuate branches of the β-ketoadipate pathway. Enzymatic study of benzoate grown cultures confirmed the activity of the ortho-catechol branch of the β-ketoadipate pathway. Even high concentrations of benzoate did not show increased stress protein synthesis, testifying to its extremophilic nature capable of surviving in harsh conditions. This ability of Pseudomonas aeruginosa san ai to efficiently degrade benzoate can provide a wide range of use of this strain in environmental and agricultural application.
PB  - Elsevier
T2  - Microbiological Research
T1  - A study of the flexibility of the carbon catabolic pathways of extremophilic  P. aeruginosa san ai exposed to benzoate versus glucose as sole carbon  sources by multi omics analytical platform
VL  - 259
SP  - 126998
DO  - 10.1016/j.micres.2022.126998
ER  - 

@article{
author = "Medić, Ana and Huttmann, Nico and Lješević, Marija and Risha, Yousef and Berezovski, Maxim and Minić, Zoran and Karadžić, Ivanka",
year = "2022",
abstract = "Polyextremophilic, hydrocarbonoclastic Pseudomonas aeruginosa san ai can survive under extreme environmental challenges in the presence of a variety of pollutants such as organic solvents and hydrocarbons, particularly aromatics, heavy metals, and high pH. To date, the metabolic plasticity of the extremophilic P. aeruginosa, has not been sufficiently studied in regard to the effect of changing carbon sources. Therefore, the present study explores the carbon metabolic pathways of polyextremophilic P. aeruginosa san ai grown on sodium benzoate versus glucose and its potential for aromatic degradation. P. aeruginosa san ai removed/metabolised early 430 mg/L of benzoate for 48 h, demonstrating a high capacity for aromatic degradation. Comparative functional proteomics, targeted metabolomics and genomics analytical approaches were employed to study the carbon metabolism of the P. aeruginosa san ai. Functional proteomic study of selected enzymes participating in the β-ketoadipate and the Entner-Doudoroff pathways revealed a metabolic reconfiguration induced by benzoate compared to glucose. Metabolome analysis implied the existence of both catechol and protocatechuate branches of the β-ketoadipate pathway. Enzymatic study of benzoate grown cultures confirmed the activity of the ortho-catechol branch of the β-ketoadipate pathway. Even high concentrations of benzoate did not show increased stress protein synthesis, testifying to its extremophilic nature capable of surviving in harsh conditions. This ability of Pseudomonas aeruginosa san ai to efficiently degrade benzoate can provide a wide range of use of this strain in environmental and agricultural application.",
publisher = "Elsevier",
journal = "Microbiological Research",
title = "A study of the flexibility of the carbon catabolic pathways of extremophilic  P. aeruginosa san ai exposed to benzoate versus glucose as sole carbon  sources by multi omics analytical platform",
volume = "259",
pages = "126998",
doi = "10.1016/j.micres.2022.126998"
}

Medić, A., Huttmann, N., Lješević, M., Risha, Y., Berezovski, M., Minić, Z.,& Karadžić, I.. (2022). A study of the flexibility of the carbon catabolic pathways of extremophilic  P. aeruginosa san ai exposed to benzoate versus glucose as sole carbon  sources by multi omics analytical platform. in Microbiological Research
Elsevier., 259, 126998.
https://doi.org/10.1016/j.micres.2022.126998

Medić A, Huttmann N, Lješević M, Risha Y, Berezovski M, Minić Z, Karadžić I. A study of the flexibility of the carbon catabolic pathways of extremophilic  P. aeruginosa san ai exposed to benzoate versus glucose as sole carbon  sources by multi omics analytical platform. in Microbiological Research. 2022;259:126998.
doi:10.1016/j.micres.2022.126998 .

Medić, Ana, Huttmann, Nico, Lješević, Marija, Risha, Yousef, Berezovski, Maxim, Minić, Zoran, Karadžić, Ivanka, "A study of the flexibility of the carbon catabolic pathways of extremophilic  P. aeruginosa san ai exposed to benzoate versus glucose as sole carbon  sources by multi omics analytical platform" in Microbiological Research, 259 (2022):126998,
https://doi.org/10.1016/j.micres.2022.126998 . .

TY  - JOUR
AU  - Medić, Ana
AU  - Huttmann, Nico
AU  - Lješević, Marija
AU  - Risha, Yousef
AU  - Berezovski, Maxim
AU  - Minić, Zoran
AU  - Karadžić, Ivanka
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5190
AB  - Polyextremophilic, hydrocarbonoclastic Pseudomonas aeruginosa san ai can survive under extreme environmental challenges in the presence of a variety of pollutants such as organic solvents and hydrocarbons, particularly aromatics, heavy metals, and high pH. To date, the metabolic plasticity of the extremophilic P. aeruginosa, has not been sufficiently studied in regard to the effect of changing carbon sources. Therefore, the present study explores the carbon metabolic pathways of polyextremophilic P. aeruginosa san ai grown on sodium benzoate versus glucose and its potential for aromatic degradation. P. aeruginosa san ai removed/metabolised early 430 mg/L of benzoate for 48 h, demonstrating a high capacity for aromatic degradation. Comparative functional proteomics, targeted metabolomics and genomics analytical approaches were employed to study the carbon metabolism of the P. aeruginosa san ai. Functional proteomic study of selected enzymes participating in the β-ketoadipate and the Entner-Doudoroff pathways revealed a metabolic reconfiguration induced by benzoate compared to glucose. Metabolome analysis implied the existence of both catechol and protocatechuate branches of the β-ketoadipate pathway. Enzymatic study of benzoate grown cultures confirmed the activity of the ortho-catechol branch of the β-ketoadipate pathway. Even high concentrations of benzoate did not show increased stress protein synthesis, testifying to its extremophilic nature capable of surviving in harsh conditions. This ability of Pseudomonas aeruginosa san ai to efficiently degrade benzoate can provide a wide range of use of this strain in environmental and agricultural application.
PB  - Elsevier
T2  - Microbiological Research
T1  - A study of the flexibility of the carbon catabolic pathways of extremophilic  P. aeruginosa san ai exposed to benzoate versus glucose as sole carbon  sources by multi omics analytical platform
VL  - 259
SP  - 126998
DO  - 10.1016/j.micres.2022.126998
ER  - 

@article{
author = "Medić, Ana and Huttmann, Nico and Lješević, Marija and Risha, Yousef and Berezovski, Maxim and Minić, Zoran and Karadžić, Ivanka",
year = "2022",
abstract = "Polyextremophilic, hydrocarbonoclastic Pseudomonas aeruginosa san ai can survive under extreme environmental challenges in the presence of a variety of pollutants such as organic solvents and hydrocarbons, particularly aromatics, heavy metals, and high pH. To date, the metabolic plasticity of the extremophilic P. aeruginosa, has not been sufficiently studied in regard to the effect of changing carbon sources. Therefore, the present study explores the carbon metabolic pathways of polyextremophilic P. aeruginosa san ai grown on sodium benzoate versus glucose and its potential for aromatic degradation. P. aeruginosa san ai removed/metabolised early 430 mg/L of benzoate for 48 h, demonstrating a high capacity for aromatic degradation. Comparative functional proteomics, targeted metabolomics and genomics analytical approaches were employed to study the carbon metabolism of the P. aeruginosa san ai. Functional proteomic study of selected enzymes participating in the β-ketoadipate and the Entner-Doudoroff pathways revealed a metabolic reconfiguration induced by benzoate compared to glucose. Metabolome analysis implied the existence of both catechol and protocatechuate branches of the β-ketoadipate pathway. Enzymatic study of benzoate grown cultures confirmed the activity of the ortho-catechol branch of the β-ketoadipate pathway. Even high concentrations of benzoate did not show increased stress protein synthesis, testifying to its extremophilic nature capable of surviving in harsh conditions. This ability of Pseudomonas aeruginosa san ai to efficiently degrade benzoate can provide a wide range of use of this strain in environmental and agricultural application.",
publisher = "Elsevier",
journal = "Microbiological Research",
title = "A study of the flexibility of the carbon catabolic pathways of extremophilic  P. aeruginosa san ai exposed to benzoate versus glucose as sole carbon  sources by multi omics analytical platform",
volume = "259",
pages = "126998",
doi = "10.1016/j.micres.2022.126998"
}

Medić, A., Huttmann, N., Lješević, M., Risha, Y., Berezovski, M., Minić, Z.,& Karadžić, I.. (2022). A study of the flexibility of the carbon catabolic pathways of extremophilic  P. aeruginosa san ai exposed to benzoate versus glucose as sole carbon  sources by multi omics analytical platform. in Microbiological Research
Elsevier., 259, 126998.
https://doi.org/10.1016/j.micres.2022.126998

Medić A, Huttmann N, Lješević M, Risha Y, Berezovski M, Minić Z, Karadžić I. A study of the flexibility of the carbon catabolic pathways of extremophilic  P. aeruginosa san ai exposed to benzoate versus glucose as sole carbon  sources by multi omics analytical platform. in Microbiological Research. 2022;259:126998.
doi:10.1016/j.micres.2022.126998 .

Medić, Ana, Huttmann, Nico, Lješević, Marija, Risha, Yousef, Berezovski, Maxim, Minić, Zoran, Karadžić, Ivanka, "A study of the flexibility of the carbon catabolic pathways of extremophilic  P. aeruginosa san ai exposed to benzoate versus glucose as sole carbon  sources by multi omics analytical platform" in Microbiological Research, 259 (2022):126998,
https://doi.org/10.1016/j.micres.2022.126998 . .

TY  - JOUR
AU  - Medić, Ana
AU  - Lješević, Marija
AU  - Inui, Hideyuku
AU  - Beškoski, Vladimir
AU  - Kojić, Ivan
AU  - Stojanović, Ksenija
AU  - Karadžić, Ivanka
PY  - 2020
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3576
AB  - Pseudomonas aeruginosa san ai, an alkaliphilic, metallotolerant bacterium, degraded individual selected
petroleum compounds, i.e., n-alkanes (n-hexadecane, n-nonadecane) and polycyclic aromatic
hydrocarbons (fluorene, phenanthrene, pyrene) with efficiency of 80%, 98%, 96%, 50% and 41%,
respectively, at initial concentrations of 20 mg L 1 and in seven days. P. aeruginosa san ai showed a high
biodegradative capacity on complex hydrocarbon mixtures, the aliphatic and aromatic fractions from
crude oil. The efficiency of P. aeruginosa san ai degradation of crude oil fractions in seven days reached
stage 3–4 of the oil biodegradation scale, which ranges from 0 (no biodegradation) to 10 (maximum
biodegradation). Identified metabolites concomitant with genomic and enzymatic data indicated the
terminal oxidation pathway for the n-alkane degradation, and the salicylate and phthalate pathways for
fluorene biodegradation. Polyextremophilic P. aeruginosa san ai, as a biosurfactant producer with
multidegradative capacity for hydrocarbons, can be used in an improved strategy for environmental
bioremediation of hydrocarbon-contaminated sites, including extreme habitats characterized by low or
elevated temperatures, acidic or alkaline pH or high concentrations of heavy metals.
PB  - Royal Society  of Chemistry
T2  - RSC Advances
T1  - Efficient biodegradation of petroleum n-alkanes and polycyclic aromatic hydrocarbons by polyextremophilic Pseudomonas aeruginosa san ai with multidegradative capacity
VL  - 10
IS  - 24
SP  - 14060
EP  - 14070
DO  - 10.1039/c9ra10371f
ER  - 

@article{
author = "Medić, Ana and Lješević, Marija and Inui, Hideyuku and Beškoski, Vladimir and Kojić, Ivan and Stojanović, Ksenija and Karadžić, Ivanka",
year = "2020",
abstract = "Pseudomonas aeruginosa san ai, an alkaliphilic, metallotolerant bacterium, degraded individual selected
petroleum compounds, i.e., n-alkanes (n-hexadecane, n-nonadecane) and polycyclic aromatic
hydrocarbons (fluorene, phenanthrene, pyrene) with efficiency of 80%, 98%, 96%, 50% and 41%,
respectively, at initial concentrations of 20 mg L 1 and in seven days. P. aeruginosa san ai showed a high
biodegradative capacity on complex hydrocarbon mixtures, the aliphatic and aromatic fractions from
crude oil. The efficiency of P. aeruginosa san ai degradation of crude oil fractions in seven days reached
stage 3–4 of the oil biodegradation scale, which ranges from 0 (no biodegradation) to 10 (maximum
biodegradation). Identified metabolites concomitant with genomic and enzymatic data indicated the
terminal oxidation pathway for the n-alkane degradation, and the salicylate and phthalate pathways for
fluorene biodegradation. Polyextremophilic P. aeruginosa san ai, as a biosurfactant producer with
multidegradative capacity for hydrocarbons, can be used in an improved strategy for environmental
bioremediation of hydrocarbon-contaminated sites, including extreme habitats characterized by low or
elevated temperatures, acidic or alkaline pH or high concentrations of heavy metals.",
publisher = "Royal Society  of Chemistry",
journal = "RSC Advances",
title = "Efficient biodegradation of petroleum n-alkanes and polycyclic aromatic hydrocarbons by polyextremophilic Pseudomonas aeruginosa san ai with multidegradative capacity",
volume = "10",
number = "24",
pages = "14060-14070",
doi = "10.1039/c9ra10371f"
}

Medić, A., Lješević, M., Inui, H., Beškoski, V., Kojić, I., Stojanović, K.,& Karadžić, I.. (2020). Efficient biodegradation of petroleum n-alkanes and polycyclic aromatic hydrocarbons by polyextremophilic Pseudomonas aeruginosa san ai with multidegradative capacity. in RSC Advances
Royal Society  of Chemistry., 10(24), 14060-14070.
https://doi.org/10.1039/c9ra10371f

Medić A, Lješević M, Inui H, Beškoski V, Kojić I, Stojanović K, Karadžić I. Efficient biodegradation of petroleum n-alkanes and polycyclic aromatic hydrocarbons by polyextremophilic Pseudomonas aeruginosa san ai with multidegradative capacity. in RSC Advances. 2020;10(24):14060-14070.
doi:10.1039/c9ra10371f .

Medić, Ana, Lješević, Marija, Inui, Hideyuku, Beškoski, Vladimir, Kojić, Ivan, Stojanović, Ksenija, Karadžić, Ivanka, "Efficient biodegradation of petroleum n-alkanes and polycyclic aromatic hydrocarbons by polyextremophilic Pseudomonas aeruginosa san ai with multidegradative capacity" in RSC Advances, 10, no. 24 (2020):14060-14070,
https://doi.org/10.1039/c9ra10371f . .

TY  - JOUR
AU  - Medić, Ana
AU  - Stojanović, Ksenija
AU  - Izrael-Živković, Lidija
AU  - Beškoski, Vladimir
AU  - Lončarević, Branka
AU  - Kazazić, Saša
AU  - Karadžić, Ivanka
PY  - 2019
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3214
AB  - The Pseudomonas aeruginosa san ai strain was investigated for its capability to degrade the 2,6-di-tertbutylphenol (2,6-DTBP) plastic additive, a hazardous and toxic substance for aquatic life. This
investigation was performed under different parameter values: 2,6-DTBP concentration, inoculum size,
pH, and temperature. The GC-MS study showed that P. aeruginosa efficiently degraded 2,6-DTBP in the
pH range of 5–8 at higher temperatures. Under exposure to 2,6-DTBP concentrations of 2, 10, and
100 mg L 1
, the strain degraded by 100, 100, and 85%, respectively, for 7 days. Crude enzyme
preparation from the biomass of P. aeruginosa san ai showed higher efficiency in 2,6-DTBP removal
than that shown by whole microbial cells. Gene encoding for the enzymes involved in the degradation of
aromatic compounds in P. aeruginosa san ai was identified. To complement the genomic data,
a comparative proteomic study of P. aeruginosa san ai grown on 2,6-DTBP or sunflower oil was
conducted by means of nanoLC-MS/MS. The presence of aromatic substances resulted in the
upregulation of aromatic ring cleavage enzymes, whose activity was confirmed by enzymatic tests;
therefore, it could be concluded that 2,6-DTBP might be degraded by ortho-ring cleavage. A
comparative proteomics study of P. aeruginosa san ai indicated that the core molecular responses to
aromatic substances can be summarized as the upregulation of proteins responsible for amino acid
metabolism with emphasized glutamate metabolism and energy production with upregulated enzymes
of glyoxylate bypass. P. aeruginosa san ai has a high capacity to efficiently degrade aromatic
compounds, and therefore its whole cells or enzymes could be used in the treatment of contaminated
areas
PB  - Royal Society of Chemistry
T2  - RSC Advances
T1  - A comprehensive study of conditions of the biodegradation of a plastic additive 2,6-di-tertbutylphenol and proteomic changes in the degrader Pseudomonas aeruginosa san ai
VL  - 9
IS  - 41
SP  - 23696
EP  - 23710
DO  - 10.1039/c9ra04298a
ER  - 

@article{
author = "Medić, Ana and Stojanović, Ksenija and Izrael-Živković, Lidija and Beškoski, Vladimir and Lončarević, Branka and Kazazić, Saša and Karadžić, Ivanka",
year = "2019",
abstract = "The Pseudomonas aeruginosa san ai strain was investigated for its capability to degrade the 2,6-di-tertbutylphenol (2,6-DTBP) plastic additive, a hazardous and toxic substance for aquatic life. This
investigation was performed under different parameter values: 2,6-DTBP concentration, inoculum size,
pH, and temperature. The GC-MS study showed that P. aeruginosa efficiently degraded 2,6-DTBP in the
pH range of 5–8 at higher temperatures. Under exposure to 2,6-DTBP concentrations of 2, 10, and
100 mg L 1
, the strain degraded by 100, 100, and 85%, respectively, for 7 days. Crude enzyme
preparation from the biomass of P. aeruginosa san ai showed higher efficiency in 2,6-DTBP removal
than that shown by whole microbial cells. Gene encoding for the enzymes involved in the degradation of
aromatic compounds in P. aeruginosa san ai was identified. To complement the genomic data,
a comparative proteomic study of P. aeruginosa san ai grown on 2,6-DTBP or sunflower oil was
conducted by means of nanoLC-MS/MS. The presence of aromatic substances resulted in the
upregulation of aromatic ring cleavage enzymes, whose activity was confirmed by enzymatic tests;
therefore, it could be concluded that 2,6-DTBP might be degraded by ortho-ring cleavage. A
comparative proteomics study of P. aeruginosa san ai indicated that the core molecular responses to
aromatic substances can be summarized as the upregulation of proteins responsible for amino acid
metabolism with emphasized glutamate metabolism and energy production with upregulated enzymes
of glyoxylate bypass. P. aeruginosa san ai has a high capacity to efficiently degrade aromatic
compounds, and therefore its whole cells or enzymes could be used in the treatment of contaminated
areas",
publisher = "Royal Society of Chemistry",
journal = "RSC Advances",
title = "A comprehensive study of conditions of the biodegradation of a plastic additive 2,6-di-tertbutylphenol and proteomic changes in the degrader Pseudomonas aeruginosa san ai",
volume = "9",
number = "41",
pages = "23696-23710",
doi = "10.1039/c9ra04298a"
}

Medić, A., Stojanović, K., Izrael-Živković, L., Beškoski, V., Lončarević, B., Kazazić, S.,& Karadžić, I.. (2019). A comprehensive study of conditions of the biodegradation of a plastic additive 2,6-di-tertbutylphenol and proteomic changes in the degrader Pseudomonas aeruginosa san ai. in RSC Advances
Royal Society of Chemistry., 9(41), 23696-23710.
https://doi.org/10.1039/c9ra04298a

Medić A, Stojanović K, Izrael-Živković L, Beškoski V, Lončarević B, Kazazić S, Karadžić I. A comprehensive study of conditions of the biodegradation of a plastic additive 2,6-di-tertbutylphenol and proteomic changes in the degrader Pseudomonas aeruginosa san ai. in RSC Advances. 2019;9(41):23696-23710.
doi:10.1039/c9ra04298a .

Medić, Ana, Stojanović, Ksenija, Izrael-Živković, Lidija, Beškoski, Vladimir, Lončarević, Branka, Kazazić, Saša, Karadžić, Ivanka, "A comprehensive study of conditions of the biodegradation of a plastic additive 2,6-di-tertbutylphenol and proteomic changes in the degrader Pseudomonas aeruginosa san ai" in RSC Advances, 9, no. 41 (2019):23696-23710,
https://doi.org/10.1039/c9ra04298a . .