TY  - JOUR
AU  - Oreščanin, Zorana
AU  - Milovanović, Slobodan R.
AU  - Spasić, Snežana
AU  - Jones, David
AU  - Spasić, Mihajlo
PY  - 2007
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3938
AB  - The conversion of nitric oxide (NO
) into its congeners nitrosonium (NO ) and nitroxyl (HNO/NO) ions may have important consequences for signal transduction and physiological responses. Manganese-containing superoxide dismutase (MnSOD) may convert
NO into its redox congeners. In our current work, we have examined the mechanism of sodium nitroprusside (SNP)-induced relaxation of arteries, with or without endothelium, from both normotensive and spontaneously hypertensive (SH) rats in the absence and
presence of MnSOD. SNP induced a greater degree of relaxation in normotensive than in SH rats. MnSOD antagonized SNPinduced relaxation and effect was greater in normotensive than hypertensive rats. However, MnSOD even potentiated SNP-induced
relaxation in mesenteric arteries with endothelium from SH rats. Our results indicate that HNO/NO-mediated relaxation is more effective in mesenteric artery smooth muscle from SH rats than from normotensive rats and that vascular dysfunction in SH rats is not
solely endothelium-derived but involves changes in vascular smooth muscl
PB  - Springer
T2  - Pharmacological Report
T1  - Different responses of mesenteric artery from normotensive and spontaneously hypertensive rats to nitric oxide and its redox congeners
VL  - 59
SP  - 315
EP  - 322
UR  - https://hdl.handle.net/21.15107/rcub_cer_3938
ER  - 

@article{
author = "Oreščanin, Zorana and Milovanović, Slobodan R. and Spasić, Snežana and Jones, David and Spasić, Mihajlo",
year = "2007",
abstract = "The conversion of nitric oxide (NO
) into its congeners nitrosonium (NO ) and nitroxyl (HNO/NO) ions may have important consequences for signal transduction and physiological responses. Manganese-containing superoxide dismutase (MnSOD) may convert
NO into its redox congeners. In our current work, we have examined the mechanism of sodium nitroprusside (SNP)-induced relaxation of arteries, with or without endothelium, from both normotensive and spontaneously hypertensive (SH) rats in the absence and
presence of MnSOD. SNP induced a greater degree of relaxation in normotensive than in SH rats. MnSOD antagonized SNPinduced relaxation and effect was greater in normotensive than hypertensive rats. However, MnSOD even potentiated SNP-induced
relaxation in mesenteric arteries with endothelium from SH rats. Our results indicate that HNO/NO-mediated relaxation is more effective in mesenteric artery smooth muscle from SH rats than from normotensive rats and that vascular dysfunction in SH rats is not
solely endothelium-derived but involves changes in vascular smooth muscl",
publisher = "Springer",
journal = "Pharmacological Report",
title = "Different responses of mesenteric artery from normotensive and spontaneously hypertensive rats to nitric oxide and its redox congeners",
volume = "59",
pages = "315-322",
url = "https://hdl.handle.net/21.15107/rcub_cer_3938"
}

Oreščanin, Z., Milovanović, S. R., Spasić, S., Jones, D.,& Spasić, M.. (2007). Different responses of mesenteric artery from normotensive and spontaneously hypertensive rats to nitric oxide and its redox congeners. in Pharmacological Report
Springer., 59, 315-322.
https://hdl.handle.net/21.15107/rcub_cer_3938

Oreščanin Z, Milovanović SR, Spasić S, Jones D, Spasić M. Different responses of mesenteric artery from normotensive and spontaneously hypertensive rats to nitric oxide and its redox congeners. in Pharmacological Report. 2007;59:315-322.
https://hdl.handle.net/21.15107/rcub_cer_3938 .

Oreščanin, Zorana, Milovanović, Slobodan R., Spasić, Snežana, Jones, David, Spasić, Mihajlo, "Different responses of mesenteric artery from normotensive and spontaneously hypertensive rats to nitric oxide and its redox congeners" in Pharmacological Report, 59 (2007):315-322,
https://hdl.handle.net/21.15107/rcub_cer_3938 .

TY  - JOUR
AU  - Milovanović, Slobodan R.
AU  - Oreščanin, Zorana
AU  - Spasić, Snežana
AU  - Miletić, Srđan
AU  - Prostran, Milica
AU  - Spasić, Mihajlo B.
PY  - 2004
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/141
AB  - In this study themolecular foundation of nitric oxide induced relaxation of arteries, with or without endothelium, of normotensive and spontanously hypertensive ratswas re-examined. With this purpose in mind, the effects of the nitric oxide donor sodium nitroprusside (NaNP), with and without manganese containing superoxide dismutase (MnSOD E.C. 1.15.1.1), on rat renal artery relaxation was strudied. The results show that the relaxation effect of NaNP is two times higher in normotensive, compared to spontaneously hypertensive rats. Similar differences exist in the relaxation effects of NaNP on isolated renal arteries without endothelium, indicating that besides the difference in the function of an endothelium, concerning basal NO production in normotensive and hypertensive rats, there is a differencewith respect to NO relaxation in the smoothmuscle that is induced by hypertension. MnSOD decreased the relaxation effect of NaNP in all the examined renal arteries, more in normotensive than in hypertensive ones regardless of the presence of an endothelium. These results show that MnSOD, by modifying the chemical versatility of NO into redox active forms - nitrosonium (NO+) and nitroxyl (NO-), produces different relaxation effects in normotensive and hypertensive arteries of rats, with or without an endothelium, potentiating the role of nitroxyl induced relaxation in sponteneously hypertensive rats. The results prove the need for the synthesis of complex NO donors, as the mechanisms of artery relaxation are different due to an endothel and smooth mouscle changes in hypertensive, as compared to normotensive rats.
AB  - U ovom radu smo pokušali da detaljnije ispitamo molekulsku osnovu azotoksid indukovane relaksacije arterija, sa i bez endotela, normotenzivnih i spontano hipertenzivnih pacova. U tu svrhu ispitivan je efekat azot-oksid (NO) donora natrijum- nitroprusida (NaNP), bez i u prisustvu superoksid-dismutaze koja sadrži mangan (MnSOD, EC 1.15.1.1) na relaksaciju renalne arterije. Rezulati pokazuju dvostruko veći relaksantni efekat NaNP kod normotenzivnih, u odnosu na spontano hipertenzivne pacove. Slična razlika postoji i u relaksantnom efektu NaNP na izolovane renalne arterije normotenzivnog i hipertenzivnog pacova kada je odstranjen endotel što ukazuje da, pored razlike u funciji endotela u odnosu na bazalnu NO produkciju kod normotenzivnih i spontano hipertenzivnih pacova, postoje i promene u glatkim mišićima indukovane hipertenzijom, u odnosu na NO relaksaciju. MnSOD kod svih grupa smanjuje relaksantni efekat SNP i to više kod normotenzivnih nego kod hipertenzivnih nezavisno od prisustva endotela. Ovi rezultati pokazuju da MnSOD menjajući hemijsku prirodu iz NaNP oslobođenog NO u redoks aktivne forme – nitrozonijum (NO+) i nitroksil (NO–) – ostvaruje različit relaksantni efekat kod normotenzivnih i kod hiperetenzivnih pacova sa i bez endotela potencirajući značaj nitroksilom indukovane relaksacije kod spontano-hipertenzivnih pacova. Dobijeni rezultati potvrđuju potrebu sinteze NO donora koji daju različite redoks-aktivne forme NO budući da se primarni mehanismi relaksacije razlikuju u zavisnosti od karakteristika endotela i glatkih mišića, kod hipertenzije u odnosu na normotenzivno stanje.
PB  - Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - Effect of MnSOD (E. coli) on the relaxation caused by sodium nitroprusside on isolated rat renal artery
T1  - Efekat MnSOD (E. coli) Na relaksaciju izolovane renalne arterije pacova izazvanu natrijum-nitro-prusidom
VL  - 69
IS  - 11
SP  - 973
EP  - 980
DO  - 10.2298/JSC0411973M
ER  - 

@article{
author = "Milovanović, Slobodan R. and Oreščanin, Zorana and Spasić, Snežana and Miletić, Srđan and Prostran, Milica and Spasić, Mihajlo B.",
year = "2004",
abstract = "In this study themolecular foundation of nitric oxide induced relaxation of arteries, with or without endothelium, of normotensive and spontanously hypertensive ratswas re-examined. With this purpose in mind, the effects of the nitric oxide donor sodium nitroprusside (NaNP), with and without manganese containing superoxide dismutase (MnSOD E.C. 1.15.1.1), on rat renal artery relaxation was strudied. The results show that the relaxation effect of NaNP is two times higher in normotensive, compared to spontaneously hypertensive rats. Similar differences exist in the relaxation effects of NaNP on isolated renal arteries without endothelium, indicating that besides the difference in the function of an endothelium, concerning basal NO production in normotensive and hypertensive rats, there is a differencewith respect to NO relaxation in the smoothmuscle that is induced by hypertension. MnSOD decreased the relaxation effect of NaNP in all the examined renal arteries, more in normotensive than in hypertensive ones regardless of the presence of an endothelium. These results show that MnSOD, by modifying the chemical versatility of NO into redox active forms - nitrosonium (NO+) and nitroxyl (NO-), produces different relaxation effects in normotensive and hypertensive arteries of rats, with or without an endothelium, potentiating the role of nitroxyl induced relaxation in sponteneously hypertensive rats. The results prove the need for the synthesis of complex NO donors, as the mechanisms of artery relaxation are different due to an endothel and smooth mouscle changes in hypertensive, as compared to normotensive rats., U ovom radu smo pokušali da detaljnije ispitamo molekulsku osnovu azotoksid indukovane relaksacije arterija, sa i bez endotela, normotenzivnih i spontano hipertenzivnih pacova. U tu svrhu ispitivan je efekat azot-oksid (NO) donora natrijum- nitroprusida (NaNP), bez i u prisustvu superoksid-dismutaze koja sadrži mangan (MnSOD, EC 1.15.1.1) na relaksaciju renalne arterije. Rezulati pokazuju dvostruko veći relaksantni efekat NaNP kod normotenzivnih, u odnosu na spontano hipertenzivne pacove. Slična razlika postoji i u relaksantnom efektu NaNP na izolovane renalne arterije normotenzivnog i hipertenzivnog pacova kada je odstranjen endotel što ukazuje da, pored razlike u funciji endotela u odnosu na bazalnu NO produkciju kod normotenzivnih i spontano hipertenzivnih pacova, postoje i promene u glatkim mišićima indukovane hipertenzijom, u odnosu na NO relaksaciju. MnSOD kod svih grupa smanjuje relaksantni efekat SNP i to više kod normotenzivnih nego kod hipertenzivnih nezavisno od prisustva endotela. Ovi rezultati pokazuju da MnSOD menjajući hemijsku prirodu iz NaNP oslobođenog NO u redoks aktivne forme – nitrozonijum (NO+) i nitroksil (NO–) – ostvaruje različit relaksantni efekat kod normotenzivnih i kod hiperetenzivnih pacova sa i bez endotela potencirajući značaj nitroksilom indukovane relaksacije kod spontano-hipertenzivnih pacova. Dobijeni rezultati potvrđuju potrebu sinteze NO donora koji daju različite redoks-aktivne forme NO budući da se primarni mehanismi relaksacije razlikuju u zavisnosti od karakteristika endotela i glatkih mišića, kod hipertenzije u odnosu na normotenzivno stanje.",
publisher = "Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Effect of MnSOD (E. coli) on the relaxation caused by sodium nitroprusside on isolated rat renal artery, Efekat MnSOD (E. coli) Na relaksaciju izolovane renalne arterije pacova izazvanu natrijum-nitro-prusidom",
volume = "69",
number = "11",
pages = "973-980",
doi = "10.2298/JSC0411973M"
}

Milovanović, S. R., Oreščanin, Z., Spasić, S., Miletić, S., Prostran, M.,& Spasić, M. B.. (2004). Effect of MnSOD (E. coli) on the relaxation caused by sodium nitroprusside on isolated rat renal artery. in Journal of the Serbian Chemical Society
Serbian Chemical Society., 69(11), 973-980.
https://doi.org/10.2298/JSC0411973M

Milovanović SR, Oreščanin Z, Spasić S, Miletić S, Prostran M, Spasić MB. Effect of MnSOD (E. coli) on the relaxation caused by sodium nitroprusside on isolated rat renal artery. in Journal of the Serbian Chemical Society. 2004;69(11):973-980.
doi:10.2298/JSC0411973M .

Milovanović, Slobodan R., Oreščanin, Zorana, Spasić, Snežana, Miletić, Srđan, Prostran, Milica, Spasić, Mihajlo B., "Effect of MnSOD (E. coli) on the relaxation caused by sodium nitroprusside on isolated rat renal artery" in Journal of the Serbian Chemical Society, 69, no. 11 (2004):973-980,
https://doi.org/10.2298/JSC0411973M . .