Mattinen, Maija-Liisa

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  • Mattinen, Maija-Liisa (3)
  • Mattinen, Maija (1)
  • Mattinen, Maija-Lisa (1)
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Brachybacterium sp CH-KOV3 isolated from an oil-polluted environment-a new producer of levan

Đurić, Aleksandra; Gojgić-Cvijović, Gordana; Jakovljević, Dragica; Kekez, Branka; Kojic, Jovana Stefanovic; Mattinen, Maija-Liisa; Harju, Inka Elina; Vrvić, Miroslav; Beškoski, Vladimir

(Elsevier, 2017)

TY  - JOUR
AU  - Đurić, Aleksandra
AU  - Gojgić-Cvijović, Gordana
AU  - Jakovljević, Dragica
AU  - Kekez, Branka
AU  - Kojic, Jovana Stefanovic
AU  - Mattinen, Maija-Liisa
AU  - Harju, Inka Elina
AU  - Vrvić, Miroslav
AU  - Beškoski, Vladimir
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2097
AB  - Various microorganisms isolated from polluted environments, such as Pseudomonas sp. and Micrococcus sp. can synthesize exopolysaccharides (EPSs) which are natural, non-toxic and biodegradable polymers. EPSs play a key role in protection of microbial cells under various external influences. For humans, these substances have potential use in many industries. EPSs can be applied as a flavor or a fragrance carrier, an emulsifier, a stabilizer, a prebiotic, an antioxidant or an antitumor agent. In this study, we characterized an environmental microorganism that produces EPS, optimized EPS production by this strain and characterized the EPS produced. Isolate CH-KOV3 was identified as Brachybacterium paraconglomeratum. The sucrose level in the growth medium greatly influenced EPS production, and the highest yield was when the microorganism was incubated in media with 500 g/L, of sucrose. The optimal temperature and pH were 28 degrees C and 7.0, respectively. The nuclear magnetic resonance (NMR) results and GC MS analysis confirmed that the residues were D-fructofuranosyl residues with beta-configuration, where fructose units are linked by beta-2,6-glycosidic bonds, with beta-2,1-linked branches. All these data indicate that the investigated EPS is a levan-type polysaccharide. Thus, it was concluded that Brachybacterium sp. CH-KOV3 could constitute a new source for production of the bioactive polysaccharide, levan.
PB  - Elsevier
T2  - International Journal of Biological Macromolecules
T1  - Brachybacterium sp CH-KOV3 isolated from an oil-polluted environment-a new producer of levan
VL  - 104
SP  - 311
EP  - 321
DO  - 10.1016/j.ijbiomac.2017.06.034
ER  - 
@article{
author = "Đurić, Aleksandra and Gojgić-Cvijović, Gordana and Jakovljević, Dragica and Kekez, Branka and Kojic, Jovana Stefanovic and Mattinen, Maija-Liisa and Harju, Inka Elina and Vrvić, Miroslav and Beškoski, Vladimir",
year = "2017",
abstract = "Various microorganisms isolated from polluted environments, such as Pseudomonas sp. and Micrococcus sp. can synthesize exopolysaccharides (EPSs) which are natural, non-toxic and biodegradable polymers. EPSs play a key role in protection of microbial cells under various external influences. For humans, these substances have potential use in many industries. EPSs can be applied as a flavor or a fragrance carrier, an emulsifier, a stabilizer, a prebiotic, an antioxidant or an antitumor agent. In this study, we characterized an environmental microorganism that produces EPS, optimized EPS production by this strain and characterized the EPS produced. Isolate CH-KOV3 was identified as Brachybacterium paraconglomeratum. The sucrose level in the growth medium greatly influenced EPS production, and the highest yield was when the microorganism was incubated in media with 500 g/L, of sucrose. The optimal temperature and pH were 28 degrees C and 7.0, respectively. The nuclear magnetic resonance (NMR) results and GC MS analysis confirmed that the residues were D-fructofuranosyl residues with beta-configuration, where fructose units are linked by beta-2,6-glycosidic bonds, with beta-2,1-linked branches. All these data indicate that the investigated EPS is a levan-type polysaccharide. Thus, it was concluded that Brachybacterium sp. CH-KOV3 could constitute a new source for production of the bioactive polysaccharide, levan.",
publisher = "Elsevier",
journal = "International Journal of Biological Macromolecules",
title = "Brachybacterium sp CH-KOV3 isolated from an oil-polluted environment-a new producer of levan",
volume = "104",
pages = "311-321",
doi = "10.1016/j.ijbiomac.2017.06.034"
}
Đurić, A., Gojgić-Cvijović, G., Jakovljević, D., Kekez, B., Kojic, J. S., Mattinen, M., Harju, I. E., Vrvić, M.,& Beškoski, V.. (2017). Brachybacterium sp CH-KOV3 isolated from an oil-polluted environment-a new producer of levan. in International Journal of Biological Macromolecules
Elsevier., 104, 311-321.
https://doi.org/10.1016/j.ijbiomac.2017.06.034
Đurić A, Gojgić-Cvijović G, Jakovljević D, Kekez B, Kojic JS, Mattinen M, Harju IE, Vrvić M, Beškoski V. Brachybacterium sp CH-KOV3 isolated from an oil-polluted environment-a new producer of levan. in International Journal of Biological Macromolecules. 2017;104:311-321.
doi:10.1016/j.ijbiomac.2017.06.034 .
Đurić, Aleksandra, Gojgić-Cvijović, Gordana, Jakovljević, Dragica, Kekez, Branka, Kojic, Jovana Stefanovic, Mattinen, Maija-Liisa, Harju, Inka Elina, Vrvić, Miroslav, Beškoski, Vladimir, "Brachybacterium sp CH-KOV3 isolated from an oil-polluted environment-a new producer of levan" in International Journal of Biological Macromolecules, 104 (2017):311-321,
https://doi.org/10.1016/j.ijbiomac.2017.06.034 . .
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Brachybacterium sp CH-KOV3 isolated from an oil-polluted environment-a new producer of levan

Đurić, Aleksandra; Gojgić-Cvijović, Gordana; Jakovljević, Dragica; Kekez, Branka; Kojic, Jovana Stefanovic; Mattinen, Maija-Liisa; Harju, Inka Elina; Vrvić, Miroslav; Beškoski, Vladimir

(Elsevier, 2017)

TY  - JOUR
AU  - Đurić, Aleksandra
AU  - Gojgić-Cvijović, Gordana
AU  - Jakovljević, Dragica
AU  - Kekez, Branka
AU  - Kojic, Jovana Stefanovic
AU  - Mattinen, Maija-Liisa
AU  - Harju, Inka Elina
AU  - Vrvić, Miroslav
AU  - Beškoski, Vladimir
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3017
AB  - Various microorganisms isolated from polluted environments, such as Pseudomonas sp. and Micrococcus sp. can synthesize exopolysaccharides (EPSs) which are natural, non-toxic and biodegradable polymers. EPSs play a key role in protection of microbial cells under various external influences. For humans, these substances have potential use in many industries. EPSs can be applied as a flavor or a fragrance carrier, an emulsifier, a stabilizer, a prebiotic, an antioxidant or an antitumor agent. In this study, we characterized an environmental microorganism that produces EPS, optimized EPS production by this strain and characterized the EPS produced. Isolate CH-KOV3 was identified as Brachybacterium paraconglomeratum. The sucrose level in the growth medium greatly influenced EPS production, and the highest yield was when the microorganism was incubated in media with 500 g/L, of sucrose. The optimal temperature and pH were 28 degrees C and 7.0, respectively. The nuclear magnetic resonance (NMR) results and GC MS analysis confirmed that the residues were D-fructofuranosyl residues with beta-configuration, where fructose units are linked by beta-2,6-glycosidic bonds, with beta-2,1-linked branches. All these data indicate that the investigated EPS is a levan-type polysaccharide. Thus, it was concluded that Brachybacterium sp. CH-KOV3 could constitute a new source for production of the bioactive polysaccharide, levan.
PB  - Elsevier
T2  - International Journal of Biological Macromolecules
T1  - Brachybacterium sp CH-KOV3 isolated from an oil-polluted environment-a new producer of levan
VL  - 104
SP  - 311
EP  - 321
DO  - 10.1016/j.ijbiomac.2017.06.034
ER  - 
@article{
author = "Đurić, Aleksandra and Gojgić-Cvijović, Gordana and Jakovljević, Dragica and Kekez, Branka and Kojic, Jovana Stefanovic and Mattinen, Maija-Liisa and Harju, Inka Elina and Vrvić, Miroslav and Beškoski, Vladimir",
year = "2017",
abstract = "Various microorganisms isolated from polluted environments, such as Pseudomonas sp. and Micrococcus sp. can synthesize exopolysaccharides (EPSs) which are natural, non-toxic and biodegradable polymers. EPSs play a key role in protection of microbial cells under various external influences. For humans, these substances have potential use in many industries. EPSs can be applied as a flavor or a fragrance carrier, an emulsifier, a stabilizer, a prebiotic, an antioxidant or an antitumor agent. In this study, we characterized an environmental microorganism that produces EPS, optimized EPS production by this strain and characterized the EPS produced. Isolate CH-KOV3 was identified as Brachybacterium paraconglomeratum. The sucrose level in the growth medium greatly influenced EPS production, and the highest yield was when the microorganism was incubated in media with 500 g/L, of sucrose. The optimal temperature and pH were 28 degrees C and 7.0, respectively. The nuclear magnetic resonance (NMR) results and GC MS analysis confirmed that the residues were D-fructofuranosyl residues with beta-configuration, where fructose units are linked by beta-2,6-glycosidic bonds, with beta-2,1-linked branches. All these data indicate that the investigated EPS is a levan-type polysaccharide. Thus, it was concluded that Brachybacterium sp. CH-KOV3 could constitute a new source for production of the bioactive polysaccharide, levan.",
publisher = "Elsevier",
journal = "International Journal of Biological Macromolecules",
title = "Brachybacterium sp CH-KOV3 isolated from an oil-polluted environment-a new producer of levan",
volume = "104",
pages = "311-321",
doi = "10.1016/j.ijbiomac.2017.06.034"
}
Đurić, A., Gojgić-Cvijović, G., Jakovljević, D., Kekez, B., Kojic, J. S., Mattinen, M., Harju, I. E., Vrvić, M.,& Beškoski, V.. (2017). Brachybacterium sp CH-KOV3 isolated from an oil-polluted environment-a new producer of levan. in International Journal of Biological Macromolecules
Elsevier., 104, 311-321.
https://doi.org/10.1016/j.ijbiomac.2017.06.034
Đurić A, Gojgić-Cvijović G, Jakovljević D, Kekez B, Kojic JS, Mattinen M, Harju IE, Vrvić M, Beškoski V. Brachybacterium sp CH-KOV3 isolated from an oil-polluted environment-a new producer of levan. in International Journal of Biological Macromolecules. 2017;104:311-321.
doi:10.1016/j.ijbiomac.2017.06.034 .
Đurić, Aleksandra, Gojgić-Cvijović, Gordana, Jakovljević, Dragica, Kekez, Branka, Kojic, Jovana Stefanovic, Mattinen, Maija-Liisa, Harju, Inka Elina, Vrvić, Miroslav, Beškoski, Vladimir, "Brachybacterium sp CH-KOV3 isolated from an oil-polluted environment-a new producer of levan" in International Journal of Biological Macromolecules, 104 (2017):311-321,
https://doi.org/10.1016/j.ijbiomac.2017.06.034 . .
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Microbial solubilization of phosphorus from phosphate rock by iron-oxidizing Acidithiobacillus sp B2

Avdalović, Jelena; Beškoski, Vladimir; Gojgić-Cvijović, Gordana; Mattinen, Maija-Liisa; Stojanovic, Mirjana; Zildzovic, Snezana; Vrvić, Miroslav

(Oxford : Pergamon-Elsevier Science Ltd, 2015)

TY  - JOUR
AU  - Avdalović, Jelena
AU  - Beškoski, Vladimir
AU  - Gojgić-Cvijović, Gordana
AU  - Mattinen, Maija-Liisa
AU  - Stojanovic, Mirjana
AU  - Zildzovic, Snezana
AU  - Vrvić, Miroslav
PY  - 2015
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1736
AB  - Procedures for solubilization of phosphorus from insoluble phosphates by acidophilic autotrophs and heterotrophs are considered to be research subjects of great importance in sustainable soil management, and could become, in the future, an alternative to current expensive methods of industrial fertilizer production. Furthermore, this biological production of fertilizer can be considered to be more environmentally friendly than current production methods. The object of this study was to investigate the possibility of phosphorus leaching from phosphate rock apatite using sulphuric acid generated from pyrite by iron-oxidizing Acidithiobacillus sp. B2, and to test the possible application of the mixture of these two minerals to produce a natural phosphate fertilizer. Iron-oxidizing Acidithiobacillus sp. B2 was isolated from copper sulphide mine wastewater (Lake Robule) in Bar, Serbia. The bacterium was identified by 16SrDNA oligonucleotide sequence and characterized using matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy (MALDI-TOF MS). The leaching experiment was carried out in laboratory conditions at 28 degrees C for a period of four weeks. The results showed that up to 34.5% of the inorganic phosphorus was leached from the test mixture when in suspension with iron-oxidizing Acidithiobacillus sp. B2, while at the same time, the degree of leaching from a control suspension (without iron-oxidizing Acidithiobacillus sp. B2) was only up to 3.8%. Due to its low cost and environmental acceptance, treatment of soil with phosphate minerals in combination with pyrite and acidophilic iron- and sulphur-oxidizing bacteria could become an attractive and alternative way to improve the quality of alkaline soils.
PB  - Oxford : Pergamon-Elsevier Science Ltd
T2  - Minerals Engineering
T1  - Microbial solubilization of phosphorus from phosphate rock by iron-oxidizing Acidithiobacillus sp B2
VL  - 72
SP  - 17
EP  - 22
DO  - 10.1016/j.mineng.2014.12.010
ER  - 
@article{
author = "Avdalović, Jelena and Beškoski, Vladimir and Gojgić-Cvijović, Gordana and Mattinen, Maija-Liisa and Stojanovic, Mirjana and Zildzovic, Snezana and Vrvić, Miroslav",
year = "2015",
abstract = "Procedures for solubilization of phosphorus from insoluble phosphates by acidophilic autotrophs and heterotrophs are considered to be research subjects of great importance in sustainable soil management, and could become, in the future, an alternative to current expensive methods of industrial fertilizer production. Furthermore, this biological production of fertilizer can be considered to be more environmentally friendly than current production methods. The object of this study was to investigate the possibility of phosphorus leaching from phosphate rock apatite using sulphuric acid generated from pyrite by iron-oxidizing Acidithiobacillus sp. B2, and to test the possible application of the mixture of these two minerals to produce a natural phosphate fertilizer. Iron-oxidizing Acidithiobacillus sp. B2 was isolated from copper sulphide mine wastewater (Lake Robule) in Bar, Serbia. The bacterium was identified by 16SrDNA oligonucleotide sequence and characterized using matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy (MALDI-TOF MS). The leaching experiment was carried out in laboratory conditions at 28 degrees C for a period of four weeks. The results showed that up to 34.5% of the inorganic phosphorus was leached from the test mixture when in suspension with iron-oxidizing Acidithiobacillus sp. B2, while at the same time, the degree of leaching from a control suspension (without iron-oxidizing Acidithiobacillus sp. B2) was only up to 3.8%. Due to its low cost and environmental acceptance, treatment of soil with phosphate minerals in combination with pyrite and acidophilic iron- and sulphur-oxidizing bacteria could become an attractive and alternative way to improve the quality of alkaline soils.",
publisher = "Oxford : Pergamon-Elsevier Science Ltd",
journal = "Minerals Engineering",
title = "Microbial solubilization of phosphorus from phosphate rock by iron-oxidizing Acidithiobacillus sp B2",
volume = "72",
pages = "17-22",
doi = "10.1016/j.mineng.2014.12.010"
}
Avdalović, J., Beškoski, V., Gojgić-Cvijović, G., Mattinen, M., Stojanovic, M., Zildzovic, S.,& Vrvić, M.. (2015). Microbial solubilization of phosphorus from phosphate rock by iron-oxidizing Acidithiobacillus sp B2. in Minerals Engineering
Oxford : Pergamon-Elsevier Science Ltd., 72, 17-22.
https://doi.org/10.1016/j.mineng.2014.12.010
Avdalović J, Beškoski V, Gojgić-Cvijović G, Mattinen M, Stojanovic M, Zildzovic S, Vrvić M. Microbial solubilization of phosphorus from phosphate rock by iron-oxidizing Acidithiobacillus sp B2. in Minerals Engineering. 2015;72:17-22.
doi:10.1016/j.mineng.2014.12.010 .
Avdalović, Jelena, Beškoski, Vladimir, Gojgić-Cvijović, Gordana, Mattinen, Maija-Liisa, Stojanovic, Mirjana, Zildzovic, Snezana, Vrvić, Miroslav, "Microbial solubilization of phosphorus from phosphate rock by iron-oxidizing Acidithiobacillus sp B2" in Minerals Engineering, 72 (2015):17-22,
https://doi.org/10.1016/j.mineng.2014.12.010 . .
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Karakterizacija bakterija izolovanih iz zagađene životne sredine pomoću MALDI-TOF-MS

Beškoski, Vladimir; Mattinen, Maija-Lisa; Milić, Jelena; Avdalović, Jelena; Ilić, Mila; Miletić, Srđan; Gojgić-Cvijović, Gordana; Vrvić, Miroslav

(Beograd: Udruženje mikrobioologa Srbije, 2013)

TY  - CONF
AU  - Beškoski, Vladimir
AU  - Mattinen, Maija-Lisa
AU  - Milić, Jelena
AU  - Avdalović, Jelena
AU  - Ilić, Mila
AU  - Miletić, Srđan
AU  - Gojgić-Cvijović, Gordana
AU  - Vrvić, Miroslav
PY  - 2013
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5054
AB  - Različite tradicionalne i moderne mikrobiološke metode omogućavaju analizu i karakterizaciju čistih bakterijskih kultura. Upotrebom MALDI-TOF MS (Matrix-assisted laser desorption/ionization-time of flight mass spectrometry) biomolekuli, kao što su proteini, peptidi, oligosaharidi i oligonukleotidi u opsegu od 400 do 350000 Da, mogu se analizirati u roku od nekoliko sekundi. Odnedavno je razvijeno i određivanje bakterijskih uzoraka kao intaktnih (celih) ćelija i bakterijskih ekstrakta.
PB  - Beograd: Udruženje mikrobioologa Srbije
C3  - IX kongres mikrobiologa Srbije, Knjiga Apstrakata, Beograd 30. maj – 01. jun 2013
T1  - Karakterizacija bakterija izolovanih iz zagađene životne sredine pomoću MALDI-TOF-MS
T1  - Characterization of bacteria isolated from polluted environment using MALDI-TOF-MS
UR  - https://hdl.handle.net/21.15107/rcub_cer_5054
ER  - 
@conference{
author = "Beškoski, Vladimir and Mattinen, Maija-Lisa and Milić, Jelena and Avdalović, Jelena and Ilić, Mila and Miletić, Srđan and Gojgić-Cvijović, Gordana and Vrvić, Miroslav",
year = "2013",
abstract = "Različite tradicionalne i moderne mikrobiološke metode omogućavaju analizu i karakterizaciju čistih bakterijskih kultura. Upotrebom MALDI-TOF MS (Matrix-assisted laser desorption/ionization-time of flight mass spectrometry) biomolekuli, kao što su proteini, peptidi, oligosaharidi i oligonukleotidi u opsegu od 400 do 350000 Da, mogu se analizirati u roku od nekoliko sekundi. Odnedavno je razvijeno i određivanje bakterijskih uzoraka kao intaktnih (celih) ćelija i bakterijskih ekstrakta.",
publisher = "Beograd: Udruženje mikrobioologa Srbije",
journal = "IX kongres mikrobiologa Srbije, Knjiga Apstrakata, Beograd 30. maj – 01. jun 2013",
title = "Karakterizacija bakterija izolovanih iz zagađene životne sredine pomoću MALDI-TOF-MS, Characterization of bacteria isolated from polluted environment using MALDI-TOF-MS",
url = "https://hdl.handle.net/21.15107/rcub_cer_5054"
}
Beškoski, V., Mattinen, M., Milić, J., Avdalović, J., Ilić, M., Miletić, S., Gojgić-Cvijović, G.,& Vrvić, M.. (2013). Karakterizacija bakterija izolovanih iz zagađene životne sredine pomoću MALDI-TOF-MS. in IX kongres mikrobiologa Srbije, Knjiga Apstrakata, Beograd 30. maj – 01. jun 2013
Beograd: Udruženje mikrobioologa Srbije..
https://hdl.handle.net/21.15107/rcub_cer_5054
Beškoski V, Mattinen M, Milić J, Avdalović J, Ilić M, Miletić S, Gojgić-Cvijović G, Vrvić M. Karakterizacija bakterija izolovanih iz zagađene životne sredine pomoću MALDI-TOF-MS. in IX kongres mikrobiologa Srbije, Knjiga Apstrakata, Beograd 30. maj – 01. jun 2013. 2013;.
https://hdl.handle.net/21.15107/rcub_cer_5054 .
Beškoski, Vladimir, Mattinen, Maija-Lisa, Milić, Jelena, Avdalović, Jelena, Ilić, Mila, Miletić, Srđan, Gojgić-Cvijović, Gordana, Vrvić, Miroslav, "Karakterizacija bakterija izolovanih iz zagađene životne sredine pomoću MALDI-TOF-MS" in IX kongres mikrobiologa Srbije, Knjiga Apstrakata, Beograd 30. maj – 01. jun 2013 (2013),
https://hdl.handle.net/21.15107/rcub_cer_5054 .

Digestibility and allergenicity assessment of enzymatically crosslinked beta-casein

Stanić, Dragana; Monogioudi, Evanthia; Dilek, Ercili; Radosavljević, Jelena; Atanasković-Marković, Marina; Vuckovic, Olga; Raija, Lantto; Mattinen, Maija; Buchert, Johanna; Ćirković-Veličković, Tanja

(Wiley, 2010)

TY  - JOUR
AU  - Stanić, Dragana
AU  - Monogioudi, Evanthia
AU  - Dilek, Ercili
AU  - Radosavljević, Jelena
AU  - Atanasković-Marković, Marina
AU  - Vuckovic, Olga
AU  - Raija, Lantto
AU  - Mattinen, Maija
AU  - Buchert, Johanna
AU  - Ćirković-Veličković, Tanja
PY  - 2010
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4168
AB  - Crosslinking enzymes are frequently used in bioprocessing of dairy products. The aim of this study was to examine the effects of enzymatic crosslinking on IgE binding, allergenicity and digestion stability of beta-casein (CN). beta-CN was crosslinked by transglutaminase, tyrosinase, mushroom tyrosinase/caffeic acid and laccase/caffeic acid. The IgE binding to beta-CN was compared in vitro by CAP inhibition assay, ELISA inhibition as well as ex vivo by basophil activation assay. Crosslinked CNs were digested by simulated gastric fluid for 15 and 60 min and obtained digests analyzed for their ability to inhibit IgE binding by CAP inhibition assay and SDS-PAGE. The ability of crosslinked CNs to activate basophils was significantly reduced in seven patients in the case of CN crosslinked by laccase and moderately reduced in the case of tyrosinase/caffeic acid crosslinked CN (in two cow's milk allergy patients tested with different allergen concentrations). The response to various crosslinked CNs differed individually among patients' sera tested by ELISA inhibition assay. The presence of caffeic acid hampered digestion by pepsin, and this effect was most pronounced for the tyrosinase/caffeic acid crosslinked CN. The laccase/caffeic acid and mushroom tyrosinase/caffeic acid had the highest potential in mitigating IgE binding and allergenicity of the beta-CN out of all investigated enzymes. The presence of a small phenolic compound also increased digestion stability of beta-CN.
PB  - Wiley
T2  - Molecular Nutrition and Food Research
T1  - Digestibility and allergenicity assessment of enzymatically crosslinked beta-casein
VL  - 54
IS  - 9
SP  - 1273
EP  - 1284
DO  - 10.1002/mnfr.200900184
ER  - 
@article{
author = "Stanić, Dragana and Monogioudi, Evanthia and Dilek, Ercili and Radosavljević, Jelena and Atanasković-Marković, Marina and Vuckovic, Olga and Raija, Lantto and Mattinen, Maija and Buchert, Johanna and Ćirković-Veličković, Tanja",
year = "2010",
abstract = "Crosslinking enzymes are frequently used in bioprocessing of dairy products. The aim of this study was to examine the effects of enzymatic crosslinking on IgE binding, allergenicity and digestion stability of beta-casein (CN). beta-CN was crosslinked by transglutaminase, tyrosinase, mushroom tyrosinase/caffeic acid and laccase/caffeic acid. The IgE binding to beta-CN was compared in vitro by CAP inhibition assay, ELISA inhibition as well as ex vivo by basophil activation assay. Crosslinked CNs were digested by simulated gastric fluid for 15 and 60 min and obtained digests analyzed for their ability to inhibit IgE binding by CAP inhibition assay and SDS-PAGE. The ability of crosslinked CNs to activate basophils was significantly reduced in seven patients in the case of CN crosslinked by laccase and moderately reduced in the case of tyrosinase/caffeic acid crosslinked CN (in two cow's milk allergy patients tested with different allergen concentrations). The response to various crosslinked CNs differed individually among patients' sera tested by ELISA inhibition assay. The presence of caffeic acid hampered digestion by pepsin, and this effect was most pronounced for the tyrosinase/caffeic acid crosslinked CN. The laccase/caffeic acid and mushroom tyrosinase/caffeic acid had the highest potential in mitigating IgE binding and allergenicity of the beta-CN out of all investigated enzymes. The presence of a small phenolic compound also increased digestion stability of beta-CN.",
publisher = "Wiley",
journal = "Molecular Nutrition and Food Research",
title = "Digestibility and allergenicity assessment of enzymatically crosslinked beta-casein",
volume = "54",
number = "9",
pages = "1273-1284",
doi = "10.1002/mnfr.200900184"
}
Stanić, D., Monogioudi, E., Dilek, E., Radosavljević, J., Atanasković-Marković, M., Vuckovic, O., Raija, L., Mattinen, M., Buchert, J.,& Ćirković-Veličković, T.. (2010). Digestibility and allergenicity assessment of enzymatically crosslinked beta-casein. in Molecular Nutrition and Food Research
Wiley., 54(9), 1273-1284.
https://doi.org/10.1002/mnfr.200900184
Stanić D, Monogioudi E, Dilek E, Radosavljević J, Atanasković-Marković M, Vuckovic O, Raija L, Mattinen M, Buchert J, Ćirković-Veličković T. Digestibility and allergenicity assessment of enzymatically crosslinked beta-casein. in Molecular Nutrition and Food Research. 2010;54(9):1273-1284.
doi:10.1002/mnfr.200900184 .
Stanić, Dragana, Monogioudi, Evanthia, Dilek, Ercili, Radosavljević, Jelena, Atanasković-Marković, Marina, Vuckovic, Olga, Raija, Lantto, Mattinen, Maija, Buchert, Johanna, Ćirković-Veličković, Tanja, "Digestibility and allergenicity assessment of enzymatically crosslinked beta-casein" in Molecular Nutrition and Food Research, 54, no. 9 (2010):1273-1284,
https://doi.org/10.1002/mnfr.200900184 . .
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