TY  - JOUR
AU  - Platanić Arizanović, Lena
AU  - Gligorijević, Nikola
AU  - Cvijetić, Ilija
AU  - Mijatović, Aleksandar
AU  - Krstić Ristivojević, Maja
AU  - Minić, Simeon
AU  - Nikolić Kokić, Aleksandra
AU  - Miljević, Čedo
AU  - Nikolić, Milan
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6482
AB  - Packed with hemoglobin, an essential protein for oxygen transport, human erythrocytes
are a suitable model system for testing the pleiotropic effects of lipophilic drugs. Our study investigated the interaction between antipsychotic drugs clozapine, ziprasidone, sertindole, and human hemoglobin under simulated physiological conditions. Analysis of protein fluorescence quenching at different temperatures and data obtained from the van’t Hoff diagram and molecular docking indicate that the interactions are static and that the tetrameric human hemoglobin has one binding site for all drugs in the central cavity near αβ  interfaces and is dominantly mediated through hydrophobic forces. The association constants were lower-moderate strength (~10^4 M^-1), the highest observed for clozapine (2.2 x 10^4 M^-1 at 25 °C). The clozapine binding showed “friendly” effects: increased α-helical content, a higher melting point, and protein protection from free radical-mediated oxidation. On the other hand, bound ziprasidone and sertindole had a slightly pro-oxidative effect, increasing ferrihemoglobin content, a possible “foe”. Since the interaction of proteins with drugs plays a vital role in their pharmacokinetic and pharmacodynamic properties, the physiological significance of the obtained findings is briefly discussed.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - International Journal of Molecular Sciences
T1  - Human Hemoglobin and Antipsychotics Clozapine, Ziprasidone and Sertindole: Friends or Foes?
VL  - 24
IS  - 10
SP  - 8921
DO  - 10.3390/ijms24108921
ER  - 

@article{
author = "Platanić Arizanović, Lena and Gligorijević, Nikola and Cvijetić, Ilija and Mijatović, Aleksandar and Krstić Ristivojević, Maja and Minić, Simeon and Nikolić Kokić, Aleksandra and Miljević, Čedo and Nikolić, Milan",
year = "2023",
abstract = "Packed with hemoglobin, an essential protein for oxygen transport, human erythrocytes
are a suitable model system for testing the pleiotropic effects of lipophilic drugs. Our study investigated the interaction between antipsychotic drugs clozapine, ziprasidone, sertindole, and human hemoglobin under simulated physiological conditions. Analysis of protein fluorescence quenching at different temperatures and data obtained from the van’t Hoff diagram and molecular docking indicate that the interactions are static and that the tetrameric human hemoglobin has one binding site for all drugs in the central cavity near αβ  interfaces and is dominantly mediated through hydrophobic forces. The association constants were lower-moderate strength (~10^4 M^-1), the highest observed for clozapine (2.2 x 10^4 M^-1 at 25 °C). The clozapine binding showed “friendly” effects: increased α-helical content, a higher melting point, and protein protection from free radical-mediated oxidation. On the other hand, bound ziprasidone and sertindole had a slightly pro-oxidative effect, increasing ferrihemoglobin content, a possible “foe”. Since the interaction of proteins with drugs plays a vital role in their pharmacokinetic and pharmacodynamic properties, the physiological significance of the obtained findings is briefly discussed.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "International Journal of Molecular Sciences",
title = "Human Hemoglobin and Antipsychotics Clozapine, Ziprasidone and Sertindole: Friends or Foes?",
volume = "24",
number = "10",
pages = "8921",
doi = "10.3390/ijms24108921"
}

Platanić Arizanović, L., Gligorijević, N., Cvijetić, I., Mijatović, A., Krstić Ristivojević, M., Minić, S., Nikolić Kokić, A., Miljević, Č.,& Nikolić, M.. (2023). Human Hemoglobin and Antipsychotics Clozapine, Ziprasidone and Sertindole: Friends or Foes?. in International Journal of Molecular Sciences
Multidisciplinary Digital Publishing Institute (MDPI)., 24(10), 8921.
https://doi.org/10.3390/ijms24108921

Platanić Arizanović L, Gligorijević N, Cvijetić I, Mijatović A, Krstić Ristivojević M, Minić S, Nikolić Kokić A, Miljević Č, Nikolić M. Human Hemoglobin and Antipsychotics Clozapine, Ziprasidone and Sertindole: Friends or Foes?. in International Journal of Molecular Sciences. 2023;24(10):8921.
doi:10.3390/ijms24108921 .

Platanić Arizanović, Lena, Gligorijević, Nikola, Cvijetić, Ilija, Mijatović, Aleksandar, Krstić Ristivojević, Maja, Minić, Simeon, Nikolić Kokić, Aleksandra, Miljević, Čedo, Nikolić, Milan, "Human Hemoglobin and Antipsychotics Clozapine, Ziprasidone and Sertindole: Friends or Foes?" in International Journal of Molecular Sciences, 24, no. 10 (2023):8921,
https://doi.org/10.3390/ijms24108921 . .

TY  - JOUR
AU  - Nikolić Kokić, Aleksandra
AU  - Tatalović, Nikola
AU  - Brkljačić, Jelena
AU  - Mijović, Milica
AU  - Nestorović, Vojkan
AU  - Mijušković, Ana
AU  - Oreščanin-Dušić, Zorana
AU  - Vidonja Uzelac, Teodora
AU  - Nikolić, Milan
AU  - Spasić, Snežana
AU  - Blagojević, Duško
AU  - Miljević, Čedo
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5557
AB  - Sexual dysfunction, as a noticeable adverse effect of atypical antipsychotic drugs (APDs)
for the treatment of schizophrenia, has not been investigated in detail. A study was undertaken
to investigate whether 28-day long treatment with clozapine, ziprasidone or sertindole (using a
recommended daily dose for atypical antipsychotic therapy), induced histopathological changes
both in rat testicles and prostate, changed the activity of the antioxidant defence system and altered blood testosterone and prolactin. Clozapine, ziprasidone and sertindole induced histopathological changes in rat testicular tissue, which could be attributed to a disturbed testicular antioxidant defence system in addition to an altered prolactin to testosterone ratio. None of the APD treatments induced histopathological changes in prostate. Our results demonstrate that APDs have the capacity to change both redox and endocrinological balance. One or both outcomes could underline testicular degeneration and disturbed spermatogenesis.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Antipsychotic Drug-Mediated Adverse Effects on Rat Testicles May Be Caused by Altered Redox and Hormonal Homeostasis
VL  - 23
IS  - 22
SP  - 13698
DO  - 10.3390/ijms232213698
ER  - 

@article{
author = "Nikolić Kokić, Aleksandra and Tatalović, Nikola and Brkljačić, Jelena and Mijović, Milica and Nestorović, Vojkan and Mijušković, Ana and Oreščanin-Dušić, Zorana and Vidonja Uzelac, Teodora and Nikolić, Milan and Spasić, Snežana and Blagojević, Duško and Miljević, Čedo",
year = "2022",
abstract = "Sexual dysfunction, as a noticeable adverse effect of atypical antipsychotic drugs (APDs)
for the treatment of schizophrenia, has not been investigated in detail. A study was undertaken
to investigate whether 28-day long treatment with clozapine, ziprasidone or sertindole (using a
recommended daily dose for atypical antipsychotic therapy), induced histopathological changes
both in rat testicles and prostate, changed the activity of the antioxidant defence system and altered blood testosterone and prolactin. Clozapine, ziprasidone and sertindole induced histopathological changes in rat testicular tissue, which could be attributed to a disturbed testicular antioxidant defence system in addition to an altered prolactin to testosterone ratio. None of the APD treatments induced histopathological changes in prostate. Our results demonstrate that APDs have the capacity to change both redox and endocrinological balance. One or both outcomes could underline testicular degeneration and disturbed spermatogenesis.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Antipsychotic Drug-Mediated Adverse Effects on Rat Testicles May Be Caused by Altered Redox and Hormonal Homeostasis",
volume = "23",
number = "22",
pages = "13698",
doi = "10.3390/ijms232213698"
}

Nikolić Kokić, A., Tatalović, N., Brkljačić, J., Mijović, M., Nestorović, V., Mijušković, A., Oreščanin-Dušić, Z., Vidonja Uzelac, T., Nikolić, M., Spasić, S., Blagojević, D.,& Miljević, Č.. (2022). Antipsychotic Drug-Mediated Adverse Effects on Rat Testicles May Be Caused by Altered Redox and Hormonal Homeostasis. in International Journal of Molecular Sciences
MDPI., 23(22), 13698.
https://doi.org/10.3390/ijms232213698

Nikolić Kokić A, Tatalović N, Brkljačić J, Mijović M, Nestorović V, Mijušković A, Oreščanin-Dušić Z, Vidonja Uzelac T, Nikolić M, Spasić S, Blagojević D, Miljević Č. Antipsychotic Drug-Mediated Adverse Effects on Rat Testicles May Be Caused by Altered Redox and Hormonal Homeostasis. in International Journal of Molecular Sciences. 2022;23(22):13698.
doi:10.3390/ijms232213698 .

Nikolić Kokić, Aleksandra, Tatalović, Nikola, Brkljačić, Jelena, Mijović, Milica, Nestorović, Vojkan, Mijušković, Ana, Oreščanin-Dušić, Zorana, Vidonja Uzelac, Teodora, Nikolić, Milan, Spasić, Snežana, Blagojević, Duško, Miljević, Čedo, "Antipsychotic Drug-Mediated Adverse Effects on Rat Testicles May Be Caused by Altered Redox and Hormonal Homeostasis" in International Journal of Molecular Sciences, 23, no. 22 (2022):13698,
https://doi.org/10.3390/ijms232213698 . .

TY  - JOUR
AU  - Đurović, Dijana
AU  - Đorđević, Zorica
AU  - Mugoša, Boban
AU  - Bajić, Borko
AU  - Nikolić Kokić, Aleksandra
AU  - Miletić, Srđan
AU  - Spasić, Snežana
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5446
AB  - Objective
In this study, iodine level in pregnant women of Montenegro and their needs for supplementation were investigated.

Methods
A urinary iodine concentration (UIC) study of 326 pregnant women between September and December 2017 in three regions of Montenegro was performed. UIC was related to creatinine (UI/Cr ratio).

Results
The median UIC (133 ± 5 μg/L) was indicative of iodine deficiency, as the WHO recommended UIC is 150–249 μg/L. The UI/Cr ratio (160 ± 6 μg/g creatinine) was just above the WHO/UNICEF/IGN recommendation. Approximately 50% of the surveyed women had a lower UIC than that recommended.

Conclusion
Iodine deficiency is present in pregnant women in Montenegro. Monitoring the UIC during routine analyses in pregnant women in Montenegro is recommended, along with iodine supplementation for individuals that need it.
PB  - Wiley
T2  - International Journal of Gynecology and Obstetrics
T1  - Half of expectant women in Montenegro show iodine deficiency, indicating that supplementation during pregnancy is necessary
DO  - 10.1002/ijgo.14370
ER  - 

@article{
author = "Đurović, Dijana and Đorđević, Zorica and Mugoša, Boban and Bajić, Borko and Nikolić Kokić, Aleksandra and Miletić, Srđan and Spasić, Snežana",
year = "2022",
abstract = "Objective
In this study, iodine level in pregnant women of Montenegro and their needs for supplementation were investigated.

Methods
A urinary iodine concentration (UIC) study of 326 pregnant women between September and December 2017 in three regions of Montenegro was performed. UIC was related to creatinine (UI/Cr ratio).

Results
The median UIC (133 ± 5 μg/L) was indicative of iodine deficiency, as the WHO recommended UIC is 150–249 μg/L. The UI/Cr ratio (160 ± 6 μg/g creatinine) was just above the WHO/UNICEF/IGN recommendation. Approximately 50% of the surveyed women had a lower UIC than that recommended.

Conclusion
Iodine deficiency is present in pregnant women in Montenegro. Monitoring the UIC during routine analyses in pregnant women in Montenegro is recommended, along with iodine supplementation for individuals that need it.",
publisher = "Wiley",
journal = "International Journal of Gynecology and Obstetrics",
title = "Half of expectant women in Montenegro show iodine deficiency, indicating that supplementation during pregnancy is necessary",
doi = "10.1002/ijgo.14370"
}

Đurović, D., Đorđević, Z., Mugoša, B., Bajić, B., Nikolić Kokić, A., Miletić, S.,& Spasić, S.. (2022). Half of expectant women in Montenegro show iodine deficiency, indicating that supplementation during pregnancy is necessary. in International Journal of Gynecology and Obstetrics
Wiley..
https://doi.org/10.1002/ijgo.14370

Đurović D, Đorđević Z, Mugoša B, Bajić B, Nikolić Kokić A, Miletić S, Spasić S. Half of expectant women in Montenegro show iodine deficiency, indicating that supplementation during pregnancy is necessary. in International Journal of Gynecology and Obstetrics. 2022;.
doi:10.1002/ijgo.14370 .

Đurović, Dijana, Đorđević, Zorica, Mugoša, Boban, Bajić, Borko, Nikolić Kokić, Aleksandra, Miletić, Srđan, Spasić, Snežana, "Half of expectant women in Montenegro show iodine deficiency, indicating that supplementation during pregnancy is necessary" in International Journal of Gynecology and Obstetrics (2022),
https://doi.org/10.1002/ijgo.14370 . .

TY  - JOUR
AU  - Miletić, Srđan
AU  - Nikolić Kokić, Aleksandra
AU  - Jovanović, Dara
AU  - Žerađanin, Aleksandra
AU  - Joksimović, Kristina
AU  - Avdalović, Jelena
AU  - Spasić, Snežana
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5846
AB  - In this research, the antioxidant activity of acidic and alkaline hydrolyzates of pectin isolated from quince (Cydonia oblonga) was investigated. The antioxidant role of quince has been known since before, but so far few papers have been published dealing with the antioxidant role of pectin hydrolyzate. In this study, quince pectin was isolated and hydrolyzed by acid and alkaline hydrolysis. Antioxidant activity was examined using a number of different methods, such as 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, Cupric Ion Reducing Antioxidant Capacity (CUPRAC) assay, iron chelators, and oxygen radical absorption capacity (ORAC). Our results, especially the ORAC test, show that depolymerization produces the most substances with antioxidant release properties in the alkaline hydrolysis of quince pectin.
PB  - Springer
T2  - Russian Journal of Bioorganic Chemistry
T1  - Investigation of the Antioxidant Role of Acidic and Alkaline Hydrolysates of Pectin Isolated from Quince (Cydonia oblonga)
DO  - 10.1134/S1068162023010193
ER  - 

@article{
author = "Miletić, Srđan and Nikolić Kokić, Aleksandra and Jovanović, Dara and Žerađanin, Aleksandra and Joksimović, Kristina and Avdalović, Jelena and Spasić, Snežana",
year = "2022",
abstract = "In this research, the antioxidant activity of acidic and alkaline hydrolyzates of pectin isolated from quince (Cydonia oblonga) was investigated. The antioxidant role of quince has been known since before, but so far few papers have been published dealing with the antioxidant role of pectin hydrolyzate. In this study, quince pectin was isolated and hydrolyzed by acid and alkaline hydrolysis. Antioxidant activity was examined using a number of different methods, such as 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, Cupric Ion Reducing Antioxidant Capacity (CUPRAC) assay, iron chelators, and oxygen radical absorption capacity (ORAC). Our results, especially the ORAC test, show that depolymerization produces the most substances with antioxidant release properties in the alkaline hydrolysis of quince pectin.",
publisher = "Springer",
journal = "Russian Journal of Bioorganic Chemistry",
title = "Investigation of the Antioxidant Role of Acidic and Alkaline Hydrolysates of Pectin Isolated from Quince (Cydonia oblonga)",
doi = "10.1134/S1068162023010193"
}

Miletić, S., Nikolić Kokić, A., Jovanović, D., Žerađanin, A., Joksimović, K., Avdalović, J.,& Spasić, S.. (2022). Investigation of the Antioxidant Role of Acidic and Alkaline Hydrolysates of Pectin Isolated from Quince (Cydonia oblonga). in Russian Journal of Bioorganic Chemistry
Springer..
https://doi.org/10.1134/S1068162023010193

Miletić S, Nikolić Kokić A, Jovanović D, Žerađanin A, Joksimović K, Avdalović J, Spasić S. Investigation of the Antioxidant Role of Acidic and Alkaline Hydrolysates of Pectin Isolated from Quince (Cydonia oblonga). in Russian Journal of Bioorganic Chemistry. 2022;.
doi:10.1134/S1068162023010193 .

Miletić, Srđan, Nikolić Kokić, Aleksandra, Jovanović, Dara, Žerađanin, Aleksandra, Joksimović, Kristina, Avdalović, Jelena, Spasić, Snežana, "Investigation of the Antioxidant Role of Acidic and Alkaline Hydrolysates of Pectin Isolated from Quince (Cydonia oblonga)" in Russian Journal of Bioorganic Chemistry (2022),
https://doi.org/10.1134/S1068162023010193 . .

TY  - JOUR
AU  - Platanović Arizanović, Lena
AU  - Nikolić-Kokić, Aleksandra
AU  - Brkljačić, Jelena
AU  - Tatalović, Nikola
AU  - Miler, Marko
AU  - Oreščanin-Dušić, Zorana
AU  - Vidonja Uzelac, Teodora
AU  - Nikolić, Milan
AU  - Milošević, Verica
AU  - Blagojević, Duško
AU  - Spasić, Snežana
AU  - Miljević, Čedo
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3967
AB  - Chronic use of atypical antipsychotics may produce hepatic damage. Atypical antipsychotics, including clozapine, sertindole, and ziprasidone, are extensively metabolized by the liver and this process generates toxic-free radical metabolic intermediates which may contribute to liver damage. The aim of this study was to investigate whether clozapine, sertindole, or ziprasidone affected hepatic antioxidant defense enzymes which consequently led to disturbed redox homeostasis. The expression and activity of antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), catalase (CAT), and glutathione-S-transferases (GST) were measured in rat livers at doses corresponding to human antipsychotic therapy. Clozapine increased activity of SOD types 1 and 2, GR and GST, but reduced CAT activity. Sertindole elevated activities of both SODs. In ziprasidone-treated rats only decreased CAT activity was found. All three antipsychotics produced mild-to-moderate hepatic histopathological changes categorized as regenerative alterations. No apparent signs of immune cell infiltration, microvesicular or macrovesicular fatty change, or hepatocytes in mitosis were observed. In conclusion, a 4-week long daily treatment with clozapine, sertindole, or ziprasidone altered hepatic antioxidant enzyme activities and induced histopathological changes in liver. The most severe alterations were noted in clozapine-treated rats. Data indicate that redox disturbances may contribute to liver dysfunction after long-term atypical antipsychotic drug treatment.
PB  - Taylor & Francis
T2  - Journal of Toxicology and Environmental Health, Part A
T1  - Effects of several atypical antipsychotics closapine, sertindole or ziprasidone on hepatic antioxidant enzymes: Possible role in drug-induced liver dysfunction
VL  - 84
IS  - 4
SP  - 173
EP  - 182
DO  - 10.1080/15287394.2020.1844827
ER  - 

@article{
author = "Platanović Arizanović, Lena and Nikolić-Kokić, Aleksandra and Brkljačić, Jelena and Tatalović, Nikola and Miler, Marko and Oreščanin-Dušić, Zorana and Vidonja Uzelac, Teodora and Nikolić, Milan and Milošević, Verica and Blagojević, Duško and Spasić, Snežana and Miljević, Čedo",
year = "2021",
abstract = "Chronic use of atypical antipsychotics may produce hepatic damage. Atypical antipsychotics, including clozapine, sertindole, and ziprasidone, are extensively metabolized by the liver and this process generates toxic-free radical metabolic intermediates which may contribute to liver damage. The aim of this study was to investigate whether clozapine, sertindole, or ziprasidone affected hepatic antioxidant defense enzymes which consequently led to disturbed redox homeostasis. The expression and activity of antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), catalase (CAT), and glutathione-S-transferases (GST) were measured in rat livers at doses corresponding to human antipsychotic therapy. Clozapine increased activity of SOD types 1 and 2, GR and GST, but reduced CAT activity. Sertindole elevated activities of both SODs. In ziprasidone-treated rats only decreased CAT activity was found. All three antipsychotics produced mild-to-moderate hepatic histopathological changes categorized as regenerative alterations. No apparent signs of immune cell infiltration, microvesicular or macrovesicular fatty change, or hepatocytes in mitosis were observed. In conclusion, a 4-week long daily treatment with clozapine, sertindole, or ziprasidone altered hepatic antioxidant enzyme activities and induced histopathological changes in liver. The most severe alterations were noted in clozapine-treated rats. Data indicate that redox disturbances may contribute to liver dysfunction after long-term atypical antipsychotic drug treatment.",
publisher = "Taylor & Francis",
journal = "Journal of Toxicology and Environmental Health, Part A",
title = "Effects of several atypical antipsychotics closapine, sertindole or ziprasidone on hepatic antioxidant enzymes: Possible role in drug-induced liver dysfunction",
volume = "84",
number = "4",
pages = "173-182",
doi = "10.1080/15287394.2020.1844827"
}

Platanović Arizanović, L., Nikolić-Kokić, A., Brkljačić, J., Tatalović, N., Miler, M., Oreščanin-Dušić, Z., Vidonja Uzelac, T., Nikolić, M., Milošević, V., Blagojević, D., Spasić, S.,& Miljević, Č.. (2021). Effects of several atypical antipsychotics closapine, sertindole or ziprasidone on hepatic antioxidant enzymes: Possible role in drug-induced liver dysfunction. in Journal of Toxicology and Environmental Health, Part A
Taylor & Francis., 84(4), 173-182.
https://doi.org/10.1080/15287394.2020.1844827

Platanović Arizanović L, Nikolić-Kokić A, Brkljačić J, Tatalović N, Miler M, Oreščanin-Dušić Z, Vidonja Uzelac T, Nikolić M, Milošević V, Blagojević D, Spasić S, Miljević Č. Effects of several atypical antipsychotics closapine, sertindole or ziprasidone on hepatic antioxidant enzymes: Possible role in drug-induced liver dysfunction. in Journal of Toxicology and Environmental Health, Part A. 2021;84(4):173-182.
doi:10.1080/15287394.2020.1844827 .

Platanović Arizanović, Lena, Nikolić-Kokić, Aleksandra, Brkljačić, Jelena, Tatalović, Nikola, Miler, Marko, Oreščanin-Dušić, Zorana, Vidonja Uzelac, Teodora, Nikolić, Milan, Milošević, Verica, Blagojević, Duško, Spasić, Snežana, Miljević, Čedo, "Effects of several atypical antipsychotics closapine, sertindole or ziprasidone on hepatic antioxidant enzymes: Possible role in drug-induced liver dysfunction" in Journal of Toxicology and Environmental Health, Part A, 84, no. 4 (2021):173-182,
https://doi.org/10.1080/15287394.2020.1844827 . .

TY  - JOUR
AU  - Spasić, Snežana
AU  - Nikolić-Kokić, Aleksandra
AU  - Miletić, Srđan
AU  - Oreščanin-Dušić, Zorana
AU  - Spasić, Mihajlo
AU  - Blagojević, Duško
AU  - Stević, Zorica
PY  - 2020
UR  - https://www.eurekaselect.com/179582/article
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3966
AB  - Radicava™ (Edaravone) was approved the Food and Drug Administration (FDA) as a new treatment for amyotrophic lateral sclerosis (ALS). Edaravone is a synthetic antioxidant that specifically targets oxidative damage interacting with lipid radicals in the cell. In ALS disease the multiple cell types are involved in devastating loss of motor neurons. Mutations and biochemical changes in various cell types jointly contribute to motor neuron death, disease onset, and disease progression. The overall mechanism of neurodegeneration in ALS is still not completely understood. Dying motor neurons have been reported to exhibit features of apoptosis. However, non-apoptotic features of dying motor neurons have also been reported such as ferroptosis. The role of Edaravone in the prevention of ferroptosis in parallel with other therapeutic approaches to ALS therapy is discussed.
PB  - Bentham Science
T2  - Current Drug Targets
T1  - Edaravone May Prevent Ferroptosis in ALS
VL  - 21
IS  - 8
SP  - 776
EP  - 780
DO  - 10.2174/1389450121666200220123305
ER  - 

@article{
author = "Spasić, Snežana and Nikolić-Kokić, Aleksandra and Miletić, Srđan and Oreščanin-Dušić, Zorana and Spasić, Mihajlo and Blagojević, Duško and Stević, Zorica",
year = "2020",
abstract = "Radicava™ (Edaravone) was approved the Food and Drug Administration (FDA) as a new treatment for amyotrophic lateral sclerosis (ALS). Edaravone is a synthetic antioxidant that specifically targets oxidative damage interacting with lipid radicals in the cell. In ALS disease the multiple cell types are involved in devastating loss of motor neurons. Mutations and biochemical changes in various cell types jointly contribute to motor neuron death, disease onset, and disease progression. The overall mechanism of neurodegeneration in ALS is still not completely understood. Dying motor neurons have been reported to exhibit features of apoptosis. However, non-apoptotic features of dying motor neurons have also been reported such as ferroptosis. The role of Edaravone in the prevention of ferroptosis in parallel with other therapeutic approaches to ALS therapy is discussed.",
publisher = "Bentham Science",
journal = "Current Drug Targets",
title = "Edaravone May Prevent Ferroptosis in ALS",
volume = "21",
number = "8",
pages = "776-780",
doi = "10.2174/1389450121666200220123305"
}

Spasić, S., Nikolić-Kokić, A., Miletić, S., Oreščanin-Dušić, Z., Spasić, M., Blagojević, D.,& Stević, Z.. (2020). Edaravone May Prevent Ferroptosis in ALS. in Current Drug Targets
Bentham Science., 21(8), 776-780.
https://doi.org/10.2174/1389450121666200220123305

Spasić S, Nikolić-Kokić A, Miletić S, Oreščanin-Dušić Z, Spasić M, Blagojević D, Stević Z. Edaravone May Prevent Ferroptosis in ALS. in Current Drug Targets. 2020;21(8):776-780.
doi:10.2174/1389450121666200220123305 .

Spasić, Snežana, Nikolić-Kokić, Aleksandra, Miletić, Srđan, Oreščanin-Dušić, Zorana, Spasić, Mihajlo, Blagojević, Duško, Stević, Zorica, "Edaravone May Prevent Ferroptosis in ALS" in Current Drug Targets, 21, no. 8 (2020):776-780,
https://doi.org/10.2174/1389450121666200220123305 . .

TY  - CONF
AU  - Lugonja, Nikoleta
AU  - Avdalović, Jelena
AU  - Stanković, Dalibor
AU  - Miletić, Srđan
AU  - Spasić, Snežana
AU  - Nikolić Kokić, Aleksandra
AU  - Beškoski, Vladimir
PY  - 2019
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5490
AB  - The aim of this research is to investigate significance of apple pectin in infant nutrition, their impact on selected bacteria of infant’s gastrointestinal microbiome and total antioxidant capacity by lectrochemical determination of changes in redox potential.
PB  - Kenes Group
C3  - Book of abstracts - First International Conference World of Microbiome: Pregnancy, Birth and Infancy, 31/10-02/11 2019, Milan, Italy
T1  - The Effects Of Pectin On The Growth And Antioxidant Properties Of Bacteria Isolated From Infant’s Gastrointestinal Microbiome - In Vitro Study
SP  - 36
UR  - https://hdl.handle.net/21.15107/rcub_cer_5490
ER  - 

@conference{
author = "Lugonja, Nikoleta and Avdalović, Jelena and Stanković, Dalibor and Miletić, Srđan and Spasić, Snežana and Nikolić Kokić, Aleksandra and Beškoski, Vladimir",
year = "2019",
abstract = "The aim of this research is to investigate significance of apple pectin in infant nutrition, their impact on selected bacteria of infant’s gastrointestinal microbiome and total antioxidant capacity by lectrochemical determination of changes in redox potential.",
publisher = "Kenes Group",
journal = "Book of abstracts - First International Conference World of Microbiome: Pregnancy, Birth and Infancy, 31/10-02/11 2019, Milan, Italy",
title = "The Effects Of Pectin On The Growth And Antioxidant Properties Of Bacteria Isolated From Infant’s Gastrointestinal Microbiome - In Vitro Study",
pages = "36",
url = "https://hdl.handle.net/21.15107/rcub_cer_5490"
}

Lugonja, N., Avdalović, J., Stanković, D., Miletić, S., Spasić, S., Nikolić Kokić, A.,& Beškoski, V.. (2019). The Effects Of Pectin On The Growth And Antioxidant Properties Of Bacteria Isolated From Infant’s Gastrointestinal Microbiome - In Vitro Study. in Book of abstracts - First International Conference World of Microbiome: Pregnancy, Birth and Infancy, 31/10-02/11 2019, Milan, Italy
Kenes Group., 36.
https://hdl.handle.net/21.15107/rcub_cer_5490

Lugonja N, Avdalović J, Stanković D, Miletić S, Spasić S, Nikolić Kokić A, Beškoski V. The Effects Of Pectin On The Growth And Antioxidant Properties Of Bacteria Isolated From Infant’s Gastrointestinal Microbiome - In Vitro Study. in Book of abstracts - First International Conference World of Microbiome: Pregnancy, Birth and Infancy, 31/10-02/11 2019, Milan, Italy. 2019;:36.
https://hdl.handle.net/21.15107/rcub_cer_5490 .

Lugonja, Nikoleta, Avdalović, Jelena, Stanković, Dalibor, Miletić, Srđan, Spasić, Snežana, Nikolić Kokić, Aleksandra, Beškoski, Vladimir, "The Effects Of Pectin On The Growth And Antioxidant Properties Of Bacteria Isolated From Infant’s Gastrointestinal Microbiome - In Vitro Study" in Book of abstracts - First International Conference World of Microbiome: Pregnancy, Birth and Infancy, 31/10-02/11 2019, Milan, Italy (2019):36,
https://hdl.handle.net/21.15107/rcub_cer_5490 .

TY  - JOUR
AU  - Uzelac, Tamara N.
AU  - Nikolić-Kokić, Aleksandra
AU  - Spasić, Snežana
AU  - Mačvanin, Mirjana T.
AU  - Nikolić, Milan
AU  - Mandić, Ljuba M.
AU  - Jovanović, Vesna B.
PY  - 2019
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3432
AB  - Antipsychotic drugs interfere with the antioxidant defense system provoking complex and often toxicological effects. Here we examined differences in plasma albumin reduced free thiol (SH) group content and its reactivity as a consequence of clozapine (CLZ) and ziprasidone (ZIP) binding. Chronic administration of CLZ reduced, whereas treatment with ZIP increased albumin-SH content in rats. Regardless of the ratio of stearic acid (SA) bound to protein, in vitro binding of ZIP to human serum albumin (HSA) increased both the SH group level and reactivity. In contrast, the effect of CLZ on HSA-SH reactivity was dependent on HSA to SA molar ratio. CLZ binding was accompanied by an increase in HSA-SH reactivity in samples with normal, but a reduction of itsreactivity level with higher SA/HSA ratio, compared to drug-free samples. We demonstrate by steady-state fluorescence quenching studies that an increase in SA binding to HSA is associated with a significant reduction of binding constant for both antipsychotics. In addition, this is the first report of quantitative characterization of ZIP binding to HSA. Our findings suggest that albumin-SH content and reactivity is modulated by ZIP towards an increased antioxidant defense capacity in circulation, as opposed to CLZ, which can contribute to the safer, more effective treatment of schizophrenia.
PB  - Elsevier
T2  - Chemico-Biological Interactions
T1  - Opposite clozapine and ziprasidone effects on the reactivity of plasma albumin SH-group are the consequence of their different binding properties dependent on protein fatty acids content
VL  - 311
SP  - 108787
DO  - 10.1016/j.cbi.2019.108787
ER  - 

@article{
author = "Uzelac, Tamara N. and Nikolić-Kokić, Aleksandra and Spasić, Snežana and Mačvanin, Mirjana T. and Nikolić, Milan and Mandić, Ljuba M. and Jovanović, Vesna B.",
year = "2019",
abstract = "Antipsychotic drugs interfere with the antioxidant defense system provoking complex and often toxicological effects. Here we examined differences in plasma albumin reduced free thiol (SH) group content and its reactivity as a consequence of clozapine (CLZ) and ziprasidone (ZIP) binding. Chronic administration of CLZ reduced, whereas treatment with ZIP increased albumin-SH content in rats. Regardless of the ratio of stearic acid (SA) bound to protein, in vitro binding of ZIP to human serum albumin (HSA) increased both the SH group level and reactivity. In contrast, the effect of CLZ on HSA-SH reactivity was dependent on HSA to SA molar ratio. CLZ binding was accompanied by an increase in HSA-SH reactivity in samples with normal, but a reduction of itsreactivity level with higher SA/HSA ratio, compared to drug-free samples. We demonstrate by steady-state fluorescence quenching studies that an increase in SA binding to HSA is associated with a significant reduction of binding constant for both antipsychotics. In addition, this is the first report of quantitative characterization of ZIP binding to HSA. Our findings suggest that albumin-SH content and reactivity is modulated by ZIP towards an increased antioxidant defense capacity in circulation, as opposed to CLZ, which can contribute to the safer, more effective treatment of schizophrenia.",
publisher = "Elsevier",
journal = "Chemico-Biological Interactions",
title = "Opposite clozapine and ziprasidone effects on the reactivity of plasma albumin SH-group are the consequence of their different binding properties dependent on protein fatty acids content",
volume = "311",
pages = "108787",
doi = "10.1016/j.cbi.2019.108787"
}

Uzelac, T. N., Nikolić-Kokić, A., Spasić, S., Mačvanin, M. T., Nikolić, M., Mandić, L. M.,& Jovanović, V. B.. (2019). Opposite clozapine and ziprasidone effects on the reactivity of plasma albumin SH-group are the consequence of their different binding properties dependent on protein fatty acids content. in Chemico-Biological Interactions
Elsevier., 311, 108787.
https://doi.org/10.1016/j.cbi.2019.108787

Uzelac TN, Nikolić-Kokić A, Spasić S, Mačvanin MT, Nikolić M, Mandić LM, Jovanović VB. Opposite clozapine and ziprasidone effects on the reactivity of plasma albumin SH-group are the consequence of their different binding properties dependent on protein fatty acids content. in Chemico-Biological Interactions. 2019;311:108787.
doi:10.1016/j.cbi.2019.108787 .

Uzelac, Tamara N., Nikolić-Kokić, Aleksandra, Spasić, Snežana, Mačvanin, Mirjana T., Nikolić, Milan, Mandić, Ljuba M., Jovanović, Vesna B., "Opposite clozapine and ziprasidone effects on the reactivity of plasma albumin SH-group are the consequence of their different binding properties dependent on protein fatty acids content" in Chemico-Biological Interactions, 311 (2019):108787,
https://doi.org/10.1016/j.cbi.2019.108787 . .

TY  - JOUR
AU  - Uzelac, Tamara N.
AU  - Nikolić-Kokić, Aleksandra
AU  - Spasić, Snežana
AU  - Mačvanin, Mirjana T.
AU  - Nikolić, Milan
AU  - Mandić, Ljuba M.
AU  - Jovanović, Vesna B.
PY  - 2019
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3053
AB  - Antipsychotic drugs interfere with the antioxidant defense system provoking complex and often toxicological effects. Here we examined differences in plasma albumin reduced free thiol (SH) group content and its reactivity as a consequence of clozapine (CLZ) and ziprasidone (ZIP) binding. Chronic administration of CLZ reduced, whereas treatment with ZIP increased albumin-SH content in rats. Regardless of the ratio of stearic acid (SA) bound to protein, in vitro binding of ZIP to human serum albumin (HSA) increased both the SH group level and reactivity. In contrast, the effect of CLZ on HSA-SH reactivity was dependent on HSA to SA molar ratio. CLZ binding was accompanied by an increase in HSA-SH reactivity in samples with normal, but a reduction of its
reactivity level with higher SA/HSA ratio, compared to drug-free samples. We demonstrate by steady-state fluorescence quenching studies that an increase in SA binding to HSA is associated with a significant reduction of binding constant for both antipsychotics. In addition, this is the first report of quantitative characterization of ZIP binding to HSA. Our findings suggest that albumin-SH content and reactivity is modulated by ZIP towards an increased antioxidant defense capacity in circulation, as opposed to CLZ, which can contribute to the safer, more effective treatment of schizophrenia.
PB  - Elsevier
T2  - Chemico-Biological Interactions
T1  - Opposite clozapine and ziprasidone effects on the reactivity of plasma albumin SH-group are the consequence of their different binding properties dependent on protein fatty acids content
VL  - 311
SP  - 108787
DO  - 10.1016/j.cbi.2019.108787
ER  - 

@article{
author = "Uzelac, Tamara N. and Nikolić-Kokić, Aleksandra and Spasić, Snežana and Mačvanin, Mirjana T. and Nikolić, Milan and Mandić, Ljuba M. and Jovanović, Vesna B.",
year = "2019",
abstract = "Antipsychotic drugs interfere with the antioxidant defense system provoking complex and often toxicological effects. Here we examined differences in plasma albumin reduced free thiol (SH) group content and its reactivity as a consequence of clozapine (CLZ) and ziprasidone (ZIP) binding. Chronic administration of CLZ reduced, whereas treatment with ZIP increased albumin-SH content in rats. Regardless of the ratio of stearic acid (SA) bound to protein, in vitro binding of ZIP to human serum albumin (HSA) increased both the SH group level and reactivity. In contrast, the effect of CLZ on HSA-SH reactivity was dependent on HSA to SA molar ratio. CLZ binding was accompanied by an increase in HSA-SH reactivity in samples with normal, but a reduction of its
reactivity level with higher SA/HSA ratio, compared to drug-free samples. We demonstrate by steady-state fluorescence quenching studies that an increase in SA binding to HSA is associated with a significant reduction of binding constant for both antipsychotics. In addition, this is the first report of quantitative characterization of ZIP binding to HSA. Our findings suggest that albumin-SH content and reactivity is modulated by ZIP towards an increased antioxidant defense capacity in circulation, as opposed to CLZ, which can contribute to the safer, more effective treatment of schizophrenia.",
publisher = "Elsevier",
journal = "Chemico-Biological Interactions",
title = "Opposite clozapine and ziprasidone effects on the reactivity of plasma albumin SH-group are the consequence of their different binding properties dependent on protein fatty acids content",
volume = "311",
pages = "108787",
doi = "10.1016/j.cbi.2019.108787"
}

Uzelac, T. N., Nikolić-Kokić, A., Spasić, S., Mačvanin, M. T., Nikolić, M., Mandić, L. M.,& Jovanović, V. B.. (2019). Opposite clozapine and ziprasidone effects on the reactivity of plasma albumin SH-group are the consequence of their different binding properties dependent on protein fatty acids content. in Chemico-Biological Interactions
Elsevier., 311, 108787.
https://doi.org/10.1016/j.cbi.2019.108787

Uzelac TN, Nikolić-Kokić A, Spasić S, Mačvanin MT, Nikolić M, Mandić LM, Jovanović VB. Opposite clozapine and ziprasidone effects on the reactivity of plasma albumin SH-group are the consequence of their different binding properties dependent on protein fatty acids content. in Chemico-Biological Interactions. 2019;311:108787.
doi:10.1016/j.cbi.2019.108787 .

Uzelac, Tamara N., Nikolić-Kokić, Aleksandra, Spasić, Snežana, Mačvanin, Mirjana T., Nikolić, Milan, Mandić, Ljuba M., Jovanović, Vesna B., "Opposite clozapine and ziprasidone effects on the reactivity of plasma albumin SH-group are the consequence of their different binding properties dependent on protein fatty acids content" in Chemico-Biological Interactions, 311 (2019):108787,
https://doi.org/10.1016/j.cbi.2019.108787 . .

TY  - CONF
AU  - Miletić, Srđan
AU  - Avdalović, Jelena
AU  - Lugonja, Nikoleta
AU  - Milić, Jelena
AU  - Nikolić Kokić, Aleksandra
AU  - Spasić, Snežana
PY  - 2019
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5152
AB  - Human milk is generally accepted as the best nutrition for newborns and has been shown to 
support the optimal microbioma, growth and development of infants. Milk from women who 
deliver prematurely differs from that of women who deliver at term. For example premature milk 
is richer in glucosaminoglycans (GAG) ,which appear to act as decoys providing binding sites 
for pathogenic bacteria to prevent adherence to the enterocyte, than term milk. A dose of 
mother’s own milk > 50 ml/kg/d decreases the risk of newborn hospital readmission rate. In this 
study we examined breast milk samples of 30 mothers of preterm infant, in their raw and 
different storage and supplementation stages. Tested samples were colostrums, transitional 
milk, mature milk (PBM), in raw stage, and in different stage of pasteurization and storage 
pasteurized mature milk, milk after 48h storage at 4C, milk after 7d storage at -20C, milk after 
7d storage at -20C and pasteurized, milk after 30d storage at -20C, milk after 30d storage at -
20C, and pasteurized; PBM supplemented with fortifier (PBM+FF) and infant formula for 
premature infants (IF PRE). It is well known that antioxidants may influence microbiome. 
According to measured total antioxidant capacity the best solution for premature babies nutrition 
is preterm mature milk with fortifier.
PB  - World of Microbiome
C3  - Book of abstracts of the First International Conference World of Microbiome: Pregnancy, Birth and Infancy, 31. October – 2. November 2019, Milan, Italy
T1  - Nutrition May Modulate Microbioma Of Preterm Neonates
SP  - 39
UR  - https://hdl.handle.net/21.15107/rcub_cer_5152
ER  - 

@conference{
author = "Miletić, Srđan and Avdalović, Jelena and Lugonja, Nikoleta and Milić, Jelena and Nikolić Kokić, Aleksandra and Spasić, Snežana",
year = "2019",
abstract = "Human milk is generally accepted as the best nutrition for newborns and has been shown to 
support the optimal microbioma, growth and development of infants. Milk from women who 
deliver prematurely differs from that of women who deliver at term. For example premature milk 
is richer in glucosaminoglycans (GAG) ,which appear to act as decoys providing binding sites 
for pathogenic bacteria to prevent adherence to the enterocyte, than term milk. A dose of 
mother’s own milk > 50 ml/kg/d decreases the risk of newborn hospital readmission rate. In this 
study we examined breast milk samples of 30 mothers of preterm infant, in their raw and 
different storage and supplementation stages. Tested samples were colostrums, transitional 
milk, mature milk (PBM), in raw stage, and in different stage of pasteurization and storage 
pasteurized mature milk, milk after 48h storage at 4C, milk after 7d storage at -20C, milk after 
7d storage at -20C and pasteurized, milk after 30d storage at -20C, milk after 30d storage at -
20C, and pasteurized; PBM supplemented with fortifier (PBM+FF) and infant formula for 
premature infants (IF PRE). It is well known that antioxidants may influence microbiome. 
According to measured total antioxidant capacity the best solution for premature babies nutrition 
is preterm mature milk with fortifier.",
publisher = "World of Microbiome",
journal = "Book of abstracts of the First International Conference World of Microbiome: Pregnancy, Birth and Infancy, 31. October – 2. November 2019, Milan, Italy",
title = "Nutrition May Modulate Microbioma Of Preterm Neonates",
pages = "39",
url = "https://hdl.handle.net/21.15107/rcub_cer_5152"
}

Miletić, S., Avdalović, J., Lugonja, N., Milić, J., Nikolić Kokić, A.,& Spasić, S.. (2019). Nutrition May Modulate Microbioma Of Preterm Neonates. in Book of abstracts of the First International Conference World of Microbiome: Pregnancy, Birth and Infancy, 31. October – 2. November 2019, Milan, Italy
World of Microbiome., 39.
https://hdl.handle.net/21.15107/rcub_cer_5152

Miletić S, Avdalović J, Lugonja N, Milić J, Nikolić Kokić A, Spasić S. Nutrition May Modulate Microbioma Of Preterm Neonates. in Book of abstracts of the First International Conference World of Microbiome: Pregnancy, Birth and Infancy, 31. October – 2. November 2019, Milan, Italy. 2019;:39.
https://hdl.handle.net/21.15107/rcub_cer_5152 .

Miletić, Srđan, Avdalović, Jelena, Lugonja, Nikoleta, Milić, Jelena, Nikolić Kokić, Aleksandra, Spasić, Snežana, "Nutrition May Modulate Microbioma Of Preterm Neonates" in Book of abstracts of the First International Conference World of Microbiome: Pregnancy, Birth and Infancy, 31. October – 2. November 2019, Milan, Italy (2019):39,
https://hdl.handle.net/21.15107/rcub_cer_5152 .

TY  - CONF
AU  - Spasić, Snežana
AU  - Miletić, Srđan
AU  - Avdalović, Jelena
AU  - Lugonja, Nikoleta
AU  - Milić, Jelena
AU  - Nikolić Kokić, Aleksandra
PY  - 2019
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5179
AB  - Transient milk from mothers of preterm infants is the most abundant milk used in banks of human milk given the capacity of preterm infants to directly consume it and the amount of milk produced. Milk banks collect, pasteurize, and freeze/store human milk. The processing might alter properties of milk which can influence microbiota of infants, but the effects have not been fully examined. Compared with colostrum and mature milk of mothers of preterm infants transient milk has lower ORP value indicating better total antioxidant capacity (TAC) than colostrum or mature milk. Main component of TAC in transient milk is vitamin C. Ascorbic acid in human milk reverts oxidation reactions because of its affinity to prevent the initiation of lipid oxidation by neutralization of singlet oxygen, or by regenerating the tocopheroxyl radical that can be produced during the lipid oxidation cascade to its native form. Milk was obtained from twenty mothers of preterm infants (gestational age 28-36 weeks; birth weight 900-2.470 g). Milk samples were obtained from 4th day postpartum to two weeks (transient). The mothers were asked to express milk between 8:00 and 10:00 AM. The main effect of transient milk processing is decrease of vitamin C and increase of urate. Our results indicate that whey of transient milk of mothers of preterm infants may be the safest fraction for preservation and very suitable for addition of fortifiers to achieve optimal effect on microbiota in preterm infant nutrition.
PB  - World of Microbiome
C3  - Book of abstracts - First International Conference World of Microbiome: Pregnancy, Birth and Infancy, 31. October – 2. November 2019, Milan, Italy
T1  - Properties Of Transient Milk From Mothers Of Preterm Infants Which Can Influence Microbiota Of Infants
SP  - 45
UR  - https://hdl.handle.net/21.15107/rcub_cer_5179
ER  - 

@conference{
author = "Spasić, Snežana and Miletić, Srđan and Avdalović, Jelena and Lugonja, Nikoleta and Milić, Jelena and Nikolić Kokić, Aleksandra",
year = "2019",
abstract = "Transient milk from mothers of preterm infants is the most abundant milk used in banks of human milk given the capacity of preterm infants to directly consume it and the amount of milk produced. Milk banks collect, pasteurize, and freeze/store human milk. The processing might alter properties of milk which can influence microbiota of infants, but the effects have not been fully examined. Compared with colostrum and mature milk of mothers of preterm infants transient milk has lower ORP value indicating better total antioxidant capacity (TAC) than colostrum or mature milk. Main component of TAC in transient milk is vitamin C. Ascorbic acid in human milk reverts oxidation reactions because of its affinity to prevent the initiation of lipid oxidation by neutralization of singlet oxygen, or by regenerating the tocopheroxyl radical that can be produced during the lipid oxidation cascade to its native form. Milk was obtained from twenty mothers of preterm infants (gestational age 28-36 weeks; birth weight 900-2.470 g). Milk samples were obtained from 4th day postpartum to two weeks (transient). The mothers were asked to express milk between 8:00 and 10:00 AM. The main effect of transient milk processing is decrease of vitamin C and increase of urate. Our results indicate that whey of transient milk of mothers of preterm infants may be the safest fraction for preservation and very suitable for addition of fortifiers to achieve optimal effect on microbiota in preterm infant nutrition.",
publisher = "World of Microbiome",
journal = "Book of abstracts - First International Conference World of Microbiome: Pregnancy, Birth and Infancy, 31. October – 2. November 2019, Milan, Italy",
title = "Properties Of Transient Milk From Mothers Of Preterm Infants Which Can Influence Microbiota Of Infants",
pages = "45",
url = "https://hdl.handle.net/21.15107/rcub_cer_5179"
}

Spasić, S., Miletić, S., Avdalović, J., Lugonja, N., Milić, J.,& Nikolić Kokić, A.. (2019). Properties Of Transient Milk From Mothers Of Preterm Infants Which Can Influence Microbiota Of Infants. in Book of abstracts - First International Conference World of Microbiome: Pregnancy, Birth and Infancy, 31. October – 2. November 2019, Milan, Italy
World of Microbiome., 45.
https://hdl.handle.net/21.15107/rcub_cer_5179

Spasić S, Miletić S, Avdalović J, Lugonja N, Milić J, Nikolić Kokić A. Properties Of Transient Milk From Mothers Of Preterm Infants Which Can Influence Microbiota Of Infants. in Book of abstracts - First International Conference World of Microbiome: Pregnancy, Birth and Infancy, 31. October – 2. November 2019, Milan, Italy. 2019;:45.
https://hdl.handle.net/21.15107/rcub_cer_5179 .

Spasić, Snežana, Miletić, Srđan, Avdalović, Jelena, Lugonja, Nikoleta, Milić, Jelena, Nikolić Kokić, Aleksandra, "Properties Of Transient Milk From Mothers Of Preterm Infants Which Can Influence Microbiota Of Infants" in Book of abstracts - First International Conference World of Microbiome: Pregnancy, Birth and Infancy, 31. October – 2. November 2019, Milan, Italy (2019):45,
https://hdl.handle.net/21.15107/rcub_cer_5179 .

TY  - CONF
AU  - Avdalović, Jelena
AU  - Lugonja, Nikoleta
AU  - Lončarević, Branka
AU  - Miletić, Srđan
AU  - Nikolić Kokić, Aleksandra
AU  - Spasić, Snežana
AU  - Beškoski, Vladimir
PY  - 2019
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5103
AB  - The aim of this study is to investigate influence of prebiotic lactulose, on selected bacteria of mother’s milk microbiome and production of gases during its fermentation.
PB  - World of Microbiome
C3  - Book of abstracts of the First International Conference World of Microbiome: Pregnancy, Birth and Infancy, 31. October – 2. November 2019, Milan, Italy
T1  - In Vitro Investigation Of Lactulose Effects On Growth And Gas Production Of Bacteria Isolated From Human Milk Microbiome,
SP  - 25
UR  - https://hdl.handle.net/21.15107/rcub_cer_5103
ER  - 

@conference{
author = "Avdalović, Jelena and Lugonja, Nikoleta and Lončarević, Branka and Miletić, Srđan and Nikolić Kokić, Aleksandra and Spasić, Snežana and Beškoski, Vladimir",
year = "2019",
abstract = "The aim of this study is to investigate influence of prebiotic lactulose, on selected bacteria of mother’s milk microbiome and production of gases during its fermentation.",
publisher = "World of Microbiome",
journal = "Book of abstracts of the First International Conference World of Microbiome: Pregnancy, Birth and Infancy, 31. October – 2. November 2019, Milan, Italy",
title = "In Vitro Investigation Of Lactulose Effects On Growth And Gas Production Of Bacteria Isolated From Human Milk Microbiome,",
pages = "25",
url = "https://hdl.handle.net/21.15107/rcub_cer_5103"
}

Avdalović, J., Lugonja, N., Lončarević, B., Miletić, S., Nikolić Kokić, A., Spasić, S.,& Beškoski, V.. (2019). In Vitro Investigation Of Lactulose Effects On Growth And Gas Production Of Bacteria Isolated From Human Milk Microbiome,. in Book of abstracts of the First International Conference World of Microbiome: Pregnancy, Birth and Infancy, 31. October – 2. November 2019, Milan, Italy
World of Microbiome., 25.
https://hdl.handle.net/21.15107/rcub_cer_5103

Avdalović J, Lugonja N, Lončarević B, Miletić S, Nikolić Kokić A, Spasić S, Beškoski V. In Vitro Investigation Of Lactulose Effects On Growth And Gas Production Of Bacteria Isolated From Human Milk Microbiome,. in Book of abstracts of the First International Conference World of Microbiome: Pregnancy, Birth and Infancy, 31. October – 2. November 2019, Milan, Italy. 2019;:25.
https://hdl.handle.net/21.15107/rcub_cer_5103 .

Avdalović, Jelena, Lugonja, Nikoleta, Lončarević, Branka, Miletić, Srđan, Nikolić Kokić, Aleksandra, Spasić, Snežana, Beškoski, Vladimir, "In Vitro Investigation Of Lactulose Effects On Growth And Gas Production Of Bacteria Isolated From Human Milk Microbiome," in Book of abstracts of the First International Conference World of Microbiome: Pregnancy, Birth and Infancy, 31. October – 2. November 2019, Milan, Italy (2019):25,
https://hdl.handle.net/21.15107/rcub_cer_5103 .

TY  - JOUR
AU  - Minić, Simeon
AU  - Jesic, Milos
AU  - Đurović, Dijana
AU  - Miletić, Srđan
AU  - Lugonja, Nikoleta
AU  - Marinković, Vesna
AU  - Nikolić-Kokić, Aleksandra
AU  - Spasić, Snežana
AU  - Vrvić, Miroslav
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2471
AB  - AimThere is a discrepancy between the amount of transitional milk produced by mothers of preterm infants and the low capacity of premature infants to consume it. This milk can be used in milk banks, but previous studies found that there are large variations in the level of host-defence proteins in individual samples of milk from mothers of premature infants, which implies that large individual variations in antioxidative defence composition are also possible. MethodsMilk samples were collected from 20 healthy mothers of preterm infants. We determined the values for non-enzymatic antioxidative capacity parameters (oxygen radical absorbance capacity (ORAC)), static oxidation-reduction potential (ORP), activities of antioxidant defence enzymes and the amount of vitamin C in whole milk, skim and whey fractions of transitional milk. ResultsThe main low-molecular-weight antioxidant in transitional milk is vitamin C and most of it is contained in whey. ORAC is higher in whole transitional milk than in skim milk and whey, and ORP is lower in whole transitional milk than that in skim milk and whey. Antioxidative enzyme activities are similar in all individual samples of transitional milk from mothers of preterm infants. ConclusionsOur results indicate that transitional milk of mothers of preterm infants shows slow individual variations in antioxidative defence composition; therefore, it can be used in human milk banks.
PB  - Wiley, Hoboken
T2  - Journal of Paediatrics and Child Health
T1  - Redox properties of transitional milk from mothers of preterm infants
VL  - 54
IS  - 2
SP  - 160
EP  - 164
DO  - 10.1111/jpc.13676
ER  - 

@article{
author = "Minić, Simeon and Jesic, Milos and Đurović, Dijana and Miletić, Srđan and Lugonja, Nikoleta and Marinković, Vesna and Nikolić-Kokić, Aleksandra and Spasić, Snežana and Vrvić, Miroslav",
year = "2018",
abstract = "AimThere is a discrepancy between the amount of transitional milk produced by mothers of preterm infants and the low capacity of premature infants to consume it. This milk can be used in milk banks, but previous studies found that there are large variations in the level of host-defence proteins in individual samples of milk from mothers of premature infants, which implies that large individual variations in antioxidative defence composition are also possible. MethodsMilk samples were collected from 20 healthy mothers of preterm infants. We determined the values for non-enzymatic antioxidative capacity parameters (oxygen radical absorbance capacity (ORAC)), static oxidation-reduction potential (ORP), activities of antioxidant defence enzymes and the amount of vitamin C in whole milk, skim and whey fractions of transitional milk. ResultsThe main low-molecular-weight antioxidant in transitional milk is vitamin C and most of it is contained in whey. ORAC is higher in whole transitional milk than in skim milk and whey, and ORP is lower in whole transitional milk than that in skim milk and whey. Antioxidative enzyme activities are similar in all individual samples of transitional milk from mothers of preterm infants. ConclusionsOur results indicate that transitional milk of mothers of preterm infants shows slow individual variations in antioxidative defence composition; therefore, it can be used in human milk banks.",
publisher = "Wiley, Hoboken",
journal = "Journal of Paediatrics and Child Health",
title = "Redox properties of transitional milk from mothers of preterm infants",
volume = "54",
number = "2",
pages = "160-164",
doi = "10.1111/jpc.13676"
}

Minić, S., Jesic, M., Đurović, D., Miletić, S., Lugonja, N., Marinković, V., Nikolić-Kokić, A., Spasić, S.,& Vrvić, M.. (2018). Redox properties of transitional milk from mothers of preterm infants. in Journal of Paediatrics and Child Health
Wiley, Hoboken., 54(2), 160-164.
https://doi.org/10.1111/jpc.13676

Minić S, Jesic M, Đurović D, Miletić S, Lugonja N, Marinković V, Nikolić-Kokić A, Spasić S, Vrvić M. Redox properties of transitional milk from mothers of preterm infants. in Journal of Paediatrics and Child Health. 2018;54(2):160-164.
doi:10.1111/jpc.13676 .

Minić, Simeon, Jesic, Milos, Đurović, Dijana, Miletić, Srđan, Lugonja, Nikoleta, Marinković, Vesna, Nikolić-Kokić, Aleksandra, Spasić, Snežana, Vrvić, Miroslav, "Redox properties of transitional milk from mothers of preterm infants" in Journal of Paediatrics and Child Health, 54, no. 2 (2018):160-164,
https://doi.org/10.1111/jpc.13676 . .

TY  - CONF
AU  - Miletić, Srđan
AU  - Lugonja, Nikoleta
AU  - Nikolić Kokić, Aleksandra
AU  - Spasić, Snežana
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5017
AB  - We prepared pectin-derived oligosaccharides (apple and citrus) and polygalacturonic acid-derived oligosaccharides, using alkaline hydrolysis by hydrogen peroxide, and analyzed them by Fourier Transform Infrared spectrometry. Furthermore, we analyzed the effects of pectin-derived oligosacharides on hydroxyl radical generating Fenton reaction using electron paramagnetic resonance spin-trapping spectroscopy, and the effects on the growth of Escherichia coli and Staphylococcus aureus in the presence of dietary relevant HO.-generating system (iron + ascorbate).
AB  - Alkalnom hidrolizom sa vodonik-peroksidom smo dobili oligosaharide iz pektina (jabuka i citrusi) i poligalakturonske oligosaharide, koje smo analizirali infracrvenom spektrometrijom sa Furijeovom transformacijom. Pored toga, elektron paramagnetnom rezonantnom spin-traping spektorskopijom smo analizirali efekat oligosaharida iz pektina na hidroksil-radikal (HO.)-generisanu Fentonovu reakciju i na rast Escherichia coli i Staphylococcus aureus u prisustvu sistema koji generiše HO.- (gvožđe + askorbat).
PB  - Belgrade: University of Belgrade
C3  - Programme & Book of Abstracts - UNIFOOD Conference, University of Belgrade 210th Anniversary, Oct 5-6, 2018, Belgrade, Serbia
T1  - Possible Mechanism of Pectin-Derived Oligosaccharides Influence on Gut Microbiota
T1  - Predloženi mehanizam uticaja oligosaharida dobijenih iz pektina na intestinalnu mikrobiotu
UR  - https://hdl.handle.net/21.15107/rcub_cer_5017
ER  - 

@conference{
author = "Miletić, Srđan and Lugonja, Nikoleta and Nikolić Kokić, Aleksandra and Spasić, Snežana",
year = "2018",
abstract = "We prepared pectin-derived oligosaccharides (apple and citrus) and polygalacturonic acid-derived oligosaccharides, using alkaline hydrolysis by hydrogen peroxide, and analyzed them by Fourier Transform Infrared spectrometry. Furthermore, we analyzed the effects of pectin-derived oligosacharides on hydroxyl radical generating Fenton reaction using electron paramagnetic resonance spin-trapping spectroscopy, and the effects on the growth of Escherichia coli and Staphylococcus aureus in the presence of dietary relevant HO.-generating system (iron + ascorbate)., Alkalnom hidrolizom sa vodonik-peroksidom smo dobili oligosaharide iz pektina (jabuka i citrusi) i poligalakturonske oligosaharide, koje smo analizirali infracrvenom spektrometrijom sa Furijeovom transformacijom. Pored toga, elektron paramagnetnom rezonantnom spin-traping spektorskopijom smo analizirali efekat oligosaharida iz pektina na hidroksil-radikal (HO.)-generisanu Fentonovu reakciju i na rast Escherichia coli i Staphylococcus aureus u prisustvu sistema koji generiše HO.- (gvožđe + askorbat).",
publisher = "Belgrade: University of Belgrade",
journal = "Programme & Book of Abstracts - UNIFOOD Conference, University of Belgrade 210th Anniversary, Oct 5-6, 2018, Belgrade, Serbia",
title = "Possible Mechanism of Pectin-Derived Oligosaccharides Influence on Gut Microbiota, Predloženi mehanizam uticaja oligosaharida dobijenih iz pektina na intestinalnu mikrobiotu",
url = "https://hdl.handle.net/21.15107/rcub_cer_5017"
}

Miletić, S., Lugonja, N., Nikolić Kokić, A.,& Spasić, S.. (2018). Possible Mechanism of Pectin-Derived Oligosaccharides Influence on Gut Microbiota. in Programme & Book of Abstracts - UNIFOOD Conference, University of Belgrade 210th Anniversary, Oct 5-6, 2018, Belgrade, Serbia
Belgrade: University of Belgrade..
https://hdl.handle.net/21.15107/rcub_cer_5017

Miletić S, Lugonja N, Nikolić Kokić A, Spasić S. Possible Mechanism of Pectin-Derived Oligosaccharides Influence on Gut Microbiota. in Programme & Book of Abstracts - UNIFOOD Conference, University of Belgrade 210th Anniversary, Oct 5-6, 2018, Belgrade, Serbia. 2018;.
https://hdl.handle.net/21.15107/rcub_cer_5017 .

Miletić, Srđan, Lugonja, Nikoleta, Nikolić Kokić, Aleksandra, Spasić, Snežana, "Possible Mechanism of Pectin-Derived Oligosaccharides Influence on Gut Microbiota" in Programme & Book of Abstracts - UNIFOOD Conference, University of Belgrade 210th Anniversary, Oct 5-6, 2018, Belgrade, Serbia (2018),
https://hdl.handle.net/21.15107/rcub_cer_5017 .

TY  - JOUR
AU  - Martinov, Jelena
AU  - Krstic, Miodrag
AU  - Spasić, Snežana
AU  - Miletić, Srđan
AU  - Stefanovic-Kojic, Jovana
AU  - Nikolić-Kokić, Aleksandra
AU  - Blagojevic, Dusko
AU  - Spasojević, Ivan
AU  - Spasić, Mihajlo
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2093
AB  - Pectin is the main soluble fiber in apples or citruses. It may be fermented by gut microbiota to metabolites showing local intestinal and systemic effects. A wide range of beneficial effects of dietary pectin includes impacts on the redox milieu and microbiota profile. We prepared pectin-derived oligosaccharides (apple (APDO) and citrus) and polygalacturonic acid-derived oligosaccharides, using alkaline hydrolysis by hydrogen peroxide, and analyzed them by Foufier Transform Infrared spectrometry. Furthermore, we analyzed the effects of pectin derived oligosaccharides on hydroxyl radical (HO center dot)-generating Fenton reaction using electron paramagnetic resonance spin-trapping spectroscopy, and the effects on the growth of Escherichia coli and Staphylococcus minus in the presence of dietary-relevant HO center dot-generating system (iron + ascorbate). The oligosaccharides react with HO center dot radical to produce carbon dioxide radical anion (CO2 center dot (-)). A comparative analysis showed that APDO has the most prominent bacteriostatic effect. This might be at least partially related to the higher capacity of APDO to produce CO2 center dot (-), which specifically targets proteins and appears to have a longer lifetime and larger diffusion radius in biological systems compared to HO center dot.
PB  - Elsevier
T2  - Food Research International
T1  - Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus
VL  - 100
SP  - 132
EP  - 136
DO  - 10.1016/j.foodres.2017.08.040
ER  - 

@article{
author = "Martinov, Jelena and Krstic, Miodrag and Spasić, Snežana and Miletić, Srđan and Stefanovic-Kojic, Jovana and Nikolić-Kokić, Aleksandra and Blagojevic, Dusko and Spasojević, Ivan and Spasić, Mihajlo",
year = "2017",
abstract = "Pectin is the main soluble fiber in apples or citruses. It may be fermented by gut microbiota to metabolites showing local intestinal and systemic effects. A wide range of beneficial effects of dietary pectin includes impacts on the redox milieu and microbiota profile. We prepared pectin-derived oligosaccharides (apple (APDO) and citrus) and polygalacturonic acid-derived oligosaccharides, using alkaline hydrolysis by hydrogen peroxide, and analyzed them by Foufier Transform Infrared spectrometry. Furthermore, we analyzed the effects of pectin derived oligosaccharides on hydroxyl radical (HO center dot)-generating Fenton reaction using electron paramagnetic resonance spin-trapping spectroscopy, and the effects on the growth of Escherichia coli and Staphylococcus minus in the presence of dietary-relevant HO center dot-generating system (iron + ascorbate). The oligosaccharides react with HO center dot radical to produce carbon dioxide radical anion (CO2 center dot (-)). A comparative analysis showed that APDO has the most prominent bacteriostatic effect. This might be at least partially related to the higher capacity of APDO to produce CO2 center dot (-), which specifically targets proteins and appears to have a longer lifetime and larger diffusion radius in biological systems compared to HO center dot.",
publisher = "Elsevier",
journal = "Food Research International",
title = "Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus",
volume = "100",
pages = "132-136",
doi = "10.1016/j.foodres.2017.08.040"
}

Martinov, J., Krstic, M., Spasić, S., Miletić, S., Stefanovic-Kojic, J., Nikolić-Kokić, A., Blagojevic, D., Spasojević, I.,& Spasić, M.. (2017). Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus. in Food Research International
Elsevier., 100, 132-136.
https://doi.org/10.1016/j.foodres.2017.08.040

Martinov J, Krstic M, Spasić S, Miletić S, Stefanovic-Kojic J, Nikolić-Kokić A, Blagojevic D, Spasojević I, Spasić M. Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus. in Food Research International. 2017;100:132-136.
doi:10.1016/j.foodres.2017.08.040 .

Martinov, Jelena, Krstic, Miodrag, Spasić, Snežana, Miletić, Srđan, Stefanovic-Kojic, Jovana, Nikolić-Kokić, Aleksandra, Blagojevic, Dusko, Spasojević, Ivan, Spasić, Mihajlo, "Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus" in Food Research International, 100 (2017):132-136,
https://doi.org/10.1016/j.foodres.2017.08.040 . .

TY  - JOUR
AU  - Martinov, Jelena
AU  - Krstic, Miodrag
AU  - Spasić, Snežana
AU  - Miletić, Srđan
AU  - Stefanovic-Kojic, Jovana
AU  - Nikolić-Kokić, Aleksandra
AU  - Blagojevic, Dusko
AU  - Spasojević, Ivan
AU  - Spasić, Mihajlo
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3043
AB  - Pectin is the main soluble fiber in apples or citruses. It may be fermented by gut microbiota to metabolites showing local intestinal and systemic effects. A wide range of beneficial effects of dietary pectin includes impacts on the redox milieu and microbiota profile. We prepared pectin-derived oligosaccharides (apple (APDO) and citrus) and polygalacturonic acid-derived oligosaccharides, using alkaline hydrolysis by hydrogen peroxide, and analyzed them by Foufier Transform Infrared spectrometry. Furthermore, we analyzed the effects of pectin derived oligosaccharides on hydroxyl radical (HO center dot)-generating Fenton reaction using electron paramagnetic resonance spin-trapping spectroscopy, and the effects on the growth of Escherichia coli and Staphylococcus minus in the presence of dietary-relevant HO center dot-generating system (iron + ascorbate). The oligosaccharides react with HO center dot radical to produce carbon dioxide radical anion (CO2 center dot (-)). A comparative analysis showed that APDO has the most prominent bacteriostatic effect. This might be at least partially related to the higher capacity of APDO to produce CO2 center dot (-), which specifically targets proteins and appears to have a longer lifetime and larger diffusion radius in biological systems compared to HO center dot.
PB  - Elsevier
T2  - Food Research International
T1  - Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus
VL  - 100
SP  - 132
EP  - 136
DO  - 10.1016/j.foodres.2017.08.040
ER  - 

@article{
author = "Martinov, Jelena and Krstic, Miodrag and Spasić, Snežana and Miletić, Srđan and Stefanovic-Kojic, Jovana and Nikolić-Kokić, Aleksandra and Blagojevic, Dusko and Spasojević, Ivan and Spasić, Mihajlo",
year = "2017",
abstract = "Pectin is the main soluble fiber in apples or citruses. It may be fermented by gut microbiota to metabolites showing local intestinal and systemic effects. A wide range of beneficial effects of dietary pectin includes impacts on the redox milieu and microbiota profile. We prepared pectin-derived oligosaccharides (apple (APDO) and citrus) and polygalacturonic acid-derived oligosaccharides, using alkaline hydrolysis by hydrogen peroxide, and analyzed them by Foufier Transform Infrared spectrometry. Furthermore, we analyzed the effects of pectin derived oligosaccharides on hydroxyl radical (HO center dot)-generating Fenton reaction using electron paramagnetic resonance spin-trapping spectroscopy, and the effects on the growth of Escherichia coli and Staphylococcus minus in the presence of dietary-relevant HO center dot-generating system (iron + ascorbate). The oligosaccharides react with HO center dot radical to produce carbon dioxide radical anion (CO2 center dot (-)). A comparative analysis showed that APDO has the most prominent bacteriostatic effect. This might be at least partially related to the higher capacity of APDO to produce CO2 center dot (-), which specifically targets proteins and appears to have a longer lifetime and larger diffusion radius in biological systems compared to HO center dot.",
publisher = "Elsevier",
journal = "Food Research International",
title = "Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus",
volume = "100",
pages = "132-136",
doi = "10.1016/j.foodres.2017.08.040"
}

Martinov, J., Krstic, M., Spasić, S., Miletić, S., Stefanovic-Kojic, J., Nikolić-Kokić, A., Blagojevic, D., Spasojević, I.,& Spasić, M.. (2017). Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus. in Food Research International
Elsevier., 100, 132-136.
https://doi.org/10.1016/j.foodres.2017.08.040

Martinov J, Krstic M, Spasić S, Miletić S, Stefanovic-Kojic J, Nikolić-Kokić A, Blagojevic D, Spasojević I, Spasić M. Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus. in Food Research International. 2017;100:132-136.
doi:10.1016/j.foodres.2017.08.040 .

Martinov, Jelena, Krstic, Miodrag, Spasić, Snežana, Miletić, Srđan, Stefanovic-Kojic, Jovana, Nikolić-Kokić, Aleksandra, Blagojevic, Dusko, Spasojević, Ivan, Spasić, Mihajlo, "Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus" in Food Research International, 100 (2017):132-136,
https://doi.org/10.1016/j.foodres.2017.08.040 . .

TY  - CONF
AU  - Lugonja, Nikoleta
AU  - Spasić, Snežana
AU  - Miletić, Srđan
AU  - Minić, Simeon
AU  - Nikolić Kokić, Aleksandra
AU  - Marinković, Vesna
AU  - Vrvić, Miroslav
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5529
AB  - Donated milk represents a desirable alternative to formula feeding, especially for preterm infants. Milk banks collect both preterm and term human milk and apply pasteurization to remove possible infectious contaminants. We examined the effects of pasteurization on redox properties of milk from mothers of preterm infants and its fractions. Electron paramagnetic resonance spectroscopy of hydroxyl-radical generating Fenton system was applied to analyze redox properties of milk from mothers of preterm infants and its fractions (skim milk and whey) and reactive products of oxidation of ascorbate and uric acid in the milk.
PB  - International Conferences on Nutrition & Growth
C3  - Abstract Book - 3rd International Conference on Nutrition & Growth, March 17-19, 2016, Vienna, Austria
T1  - Effects of pasteurization on redox properties of colostrum and milk from mothers of preterm infants
SP  - 98
UR  - https://hdl.handle.net/21.15107/rcub_cer_5529
ER  - 

@conference{
author = "Lugonja, Nikoleta and Spasić, Snežana and Miletić, Srđan and Minić, Simeon and Nikolić Kokić, Aleksandra and Marinković, Vesna and Vrvić, Miroslav",
year = "2016",
abstract = "Donated milk represents a desirable alternative to formula feeding, especially for preterm infants. Milk banks collect both preterm and term human milk and apply pasteurization to remove possible infectious contaminants. We examined the effects of pasteurization on redox properties of milk from mothers of preterm infants and its fractions. Electron paramagnetic resonance spectroscopy of hydroxyl-radical generating Fenton system was applied to analyze redox properties of milk from mothers of preterm infants and its fractions (skim milk and whey) and reactive products of oxidation of ascorbate and uric acid in the milk.",
publisher = "International Conferences on Nutrition & Growth",
journal = "Abstract Book - 3rd International Conference on Nutrition & Growth, March 17-19, 2016, Vienna, Austria",
title = "Effects of pasteurization on redox properties of colostrum and milk from mothers of preterm infants",
pages = "98",
url = "https://hdl.handle.net/21.15107/rcub_cer_5529"
}

Lugonja, N., Spasić, S., Miletić, S., Minić, S., Nikolić Kokić, A., Marinković, V.,& Vrvić, M.. (2016). Effects of pasteurization on redox properties of colostrum and milk from mothers of preterm infants. in Abstract Book - 3rd International Conference on Nutrition & Growth, March 17-19, 2016, Vienna, Austria
International Conferences on Nutrition & Growth., 98.
https://hdl.handle.net/21.15107/rcub_cer_5529

Lugonja N, Spasić S, Miletić S, Minić S, Nikolić Kokić A, Marinković V, Vrvić M. Effects of pasteurization on redox properties of colostrum and milk from mothers of preterm infants. in Abstract Book - 3rd International Conference on Nutrition & Growth, March 17-19, 2016, Vienna, Austria. 2016;:98.
https://hdl.handle.net/21.15107/rcub_cer_5529 .

Lugonja, Nikoleta, Spasić, Snežana, Miletić, Srđan, Minić, Simeon, Nikolić Kokić, Aleksandra, Marinković, Vesna, Vrvić, Miroslav, "Effects of pasteurization on redox properties of colostrum and milk from mothers of preterm infants" in Abstract Book - 3rd International Conference on Nutrition & Growth, March 17-19, 2016, Vienna, Austria (2016):98,
https://hdl.handle.net/21.15107/rcub_cer_5529 .

TY  - CONF
AU  - Lugonja, Nikoleta
AU  - Spasić, Snežana
AU  - Miletić, Srđan
AU  - Minić, Simeon
AU  - Nikolić Kokić, Aleksandra
AU  - Marinković, Vesna
AU  - Vrvić, Miroslav
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5530
AB  - Donated milk represents a desirable alternative to formula feeding, especially for preterm infants. Milk banks collect both preterm and term human milk and apply pasteurization to remove possible infectious contaminants. We examined the effects of pasteurization on redox properties of milk from mothers of preterm infants and its fractions. Electron paramagnetic resonance spectroscopy of hydroxyl-radical generating Fenton system was applied to analyze redox properties of milk from mothers of preterm infants and its fractions (skim milk and whey) and reactive products of oxidation of ascorbate and uric acid in the milk.
T1  - Effects of pasteurization on redox properties of colostrum and milk from mothers of preterm infants
UR  - https://hdl.handle.net/21.15107/rcub_cer_5530
ER  - 

@conference{
author = "Lugonja, Nikoleta and Spasić, Snežana and Miletić, Srđan and Minić, Simeon and Nikolić Kokić, Aleksandra and Marinković, Vesna and Vrvić, Miroslav",
year = "2016",
abstract = "Donated milk represents a desirable alternative to formula feeding, especially for preterm infants. Milk banks collect both preterm and term human milk and apply pasteurization to remove possible infectious contaminants. We examined the effects of pasteurization on redox properties of milk from mothers of preterm infants and its fractions. Electron paramagnetic resonance spectroscopy of hydroxyl-radical generating Fenton system was applied to analyze redox properties of milk from mothers of preterm infants and its fractions (skim milk and whey) and reactive products of oxidation of ascorbate and uric acid in the milk.",
title = "Effects of pasteurization on redox properties of colostrum and milk from mothers of preterm infants",
url = "https://hdl.handle.net/21.15107/rcub_cer_5530"
}

Lugonja, N., Spasić, S., Miletić, S., Minić, S., Nikolić Kokić, A., Marinković, V.,& Vrvić, M.. (2016). Effects of pasteurization on redox properties of colostrum and milk from mothers of preterm infants. .
https://hdl.handle.net/21.15107/rcub_cer_5530

Lugonja N, Spasić S, Miletić S, Minić S, Nikolić Kokić A, Marinković V, Vrvić M. Effects of pasteurization on redox properties of colostrum and milk from mothers of preterm infants. 2016;.
https://hdl.handle.net/21.15107/rcub_cer_5530 .

Lugonja, Nikoleta, Spasić, Snežana, Miletić, Srđan, Minić, Simeon, Nikolić Kokić, Aleksandra, Marinković, Vesna, Vrvić, Miroslav, "Effects of pasteurization on redox properties of colostrum and milk from mothers of preterm infants" (2016),
https://hdl.handle.net/21.15107/rcub_cer_5530 .

TY  - JOUR
AU  - Marinković, Vesna
AU  - Rankovic-Janevski, Milica
AU  - Spasić, Snežana
AU  - Nikolić-Kokić, Aleksandra
AU  - Lugonja, Nikoleta
AU  - Đurović, Dijana
AU  - Miletić, Srđan
AU  - Vrvić, Miroslav
AU  - Spasojević, Ivan
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2025
AB  - Objectives: Milk banks collect, pasteurize, and freeze/store human milk. The processing may alter redox properties of milk, but the effects have not been fully examined. Methods: We collected 10 mature milk and 10 colostrum samples and applied a battery of biochemical assays and electron paramagnetic resonance spectroscopy to inspect changes that milk undergoes with pasteurization and 30 days storage at -20 degrees C. Results: Pasteurization and storage of raw milk did not affect total nonenzymatic antioxidative capacity, but specific components and features were altered. Urate radical and ascorbyl radical emerge as products of exposure of milk to hydroxyl radical-generating system. Processing shifted the load of antioxidative activity from ascorbate to urate and lowered the capacity of milk to diminish hydroxyl radical. Pasteurization caused a significant drop in the activity of 2 major antioxidative enzymes-superoxide dismutase and glutathione peroxidase, whereas freezing/storage of raw milk affected only superoxide dismutase. Colostrum showed drastically higher total nonenzymatic antioxidative capacity, hydroxyl radical scavenging ability, and glutathione reductase activity compared with mature milk. Conclusions: Pasteurization and storage affect nonenzymatic and enzymatic antioxidative agents in human milk. It appears that nonenzymatic antioxidative systems in colostrum and milk are different. The effects of processing may be partially compensated by fortification/spiking with ascorbate before use.
PB  - Lippincott Williams & Wilkins, Philadelphia
T2  - Journal of Pediatric Gastroenterology and Nutrition
T1  - Antioxidative Activity of Colostrum and Human Milk: Effects of Pasteurization and Storage
VL  - 62
IS  - 6
SP  - 901
EP  - 906
DO  - 10.1097/MPG.0000000000001090
ER  - 

@article{
author = "Marinković, Vesna and Rankovic-Janevski, Milica and Spasić, Snežana and Nikolić-Kokić, Aleksandra and Lugonja, Nikoleta and Đurović, Dijana and Miletić, Srđan and Vrvić, Miroslav and Spasojević, Ivan",
year = "2016",
abstract = "Objectives: Milk banks collect, pasteurize, and freeze/store human milk. The processing may alter redox properties of milk, but the effects have not been fully examined. Methods: We collected 10 mature milk and 10 colostrum samples and applied a battery of biochemical assays and electron paramagnetic resonance spectroscopy to inspect changes that milk undergoes with pasteurization and 30 days storage at -20 degrees C. Results: Pasteurization and storage of raw milk did not affect total nonenzymatic antioxidative capacity, but specific components and features were altered. Urate radical and ascorbyl radical emerge as products of exposure of milk to hydroxyl radical-generating system. Processing shifted the load of antioxidative activity from ascorbate to urate and lowered the capacity of milk to diminish hydroxyl radical. Pasteurization caused a significant drop in the activity of 2 major antioxidative enzymes-superoxide dismutase and glutathione peroxidase, whereas freezing/storage of raw milk affected only superoxide dismutase. Colostrum showed drastically higher total nonenzymatic antioxidative capacity, hydroxyl radical scavenging ability, and glutathione reductase activity compared with mature milk. Conclusions: Pasteurization and storage affect nonenzymatic and enzymatic antioxidative agents in human milk. It appears that nonenzymatic antioxidative systems in colostrum and milk are different. The effects of processing may be partially compensated by fortification/spiking with ascorbate before use.",
publisher = "Lippincott Williams & Wilkins, Philadelphia",
journal = "Journal of Pediatric Gastroenterology and Nutrition",
title = "Antioxidative Activity of Colostrum and Human Milk: Effects of Pasteurization and Storage",
volume = "62",
number = "6",
pages = "901-906",
doi = "10.1097/MPG.0000000000001090"
}

Marinković, V., Rankovic-Janevski, M., Spasić, S., Nikolić-Kokić, A., Lugonja, N., Đurović, D., Miletić, S., Vrvić, M.,& Spasojević, I.. (2016). Antioxidative Activity of Colostrum and Human Milk: Effects of Pasteurization and Storage. in Journal of Pediatric Gastroenterology and Nutrition
Lippincott Williams & Wilkins, Philadelphia., 62(6), 901-906.
https://doi.org/10.1097/MPG.0000000000001090

Marinković V, Rankovic-Janevski M, Spasić S, Nikolić-Kokić A, Lugonja N, Đurović D, Miletić S, Vrvić M, Spasojević I. Antioxidative Activity of Colostrum and Human Milk: Effects of Pasteurization and Storage. in Journal of Pediatric Gastroenterology and Nutrition. 2016;62(6):901-906.
doi:10.1097/MPG.0000000000001090 .

Marinković, Vesna, Rankovic-Janevski, Milica, Spasić, Snežana, Nikolić-Kokić, Aleksandra, Lugonja, Nikoleta, Đurović, Dijana, Miletić, Srđan, Vrvić, Miroslav, Spasojević, Ivan, "Antioxidative Activity of Colostrum and Human Milk: Effects of Pasteurization and Storage" in Journal of Pediatric Gastroenterology and Nutrition, 62, no. 6 (2016):901-906,
https://doi.org/10.1097/MPG.0000000000001090 . .

TY  - JOUR
AU  - Bolic, Bojana
AU  - Mijuskovic, Ana
AU  - Popovic-Bijelic, Ana
AU  - Nikolić-Kokić, Aleksandra
AU  - Spasić, Snežana
AU  - Blagojevic, Dusko
AU  - Spasić, Mihajlo
AU  - Spasojević, Ivan
PY  - 2015
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1787
AB  - Interactions of hydrogen sulfide (HS-/H2S), a reducing signaling species, with superoxide dimutases (SOD) are poorly understood. We applied low-T EPR spectroscopy to examine the effects of HS-/H2S and superoxide radical anion (O-2(-)) on metallocenters of FeSOD, MnSOD, and CuZnSOD. HS-/H2S did not affect FeSOD, whereas active centers of MnSOD and CuZnSOD were open to this agent. Cu2+ was reduced to Cu1+, while manganese appears to be released from MnSOD active center. Untreated and O-2(-) treated FeSOD and MnSOD predominantly show 5 d-electron systems, i.e. Fe3+ and Mn2+. Our study provides new details on the mechanisms of (patho)physiological effects of HS-/H2S.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Nitric Oxide-Biology and Chemistry
T1  - Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study
VL  - 51
SP  - 19
EP  - 23
DO  - 10.1016/j.niox.2015.09.008
ER  - 

@article{
author = "Bolic, Bojana and Mijuskovic, Ana and Popovic-Bijelic, Ana and Nikolić-Kokić, Aleksandra and Spasić, Snežana and Blagojevic, Dusko and Spasić, Mihajlo and Spasojević, Ivan",
year = "2015",
abstract = "Interactions of hydrogen sulfide (HS-/H2S), a reducing signaling species, with superoxide dimutases (SOD) are poorly understood. We applied low-T EPR spectroscopy to examine the effects of HS-/H2S and superoxide radical anion (O-2(-)) on metallocenters of FeSOD, MnSOD, and CuZnSOD. HS-/H2S did not affect FeSOD, whereas active centers of MnSOD and CuZnSOD were open to this agent. Cu2+ was reduced to Cu1+, while manganese appears to be released from MnSOD active center. Untreated and O-2(-) treated FeSOD and MnSOD predominantly show 5 d-electron systems, i.e. Fe3+ and Mn2+. Our study provides new details on the mechanisms of (patho)physiological effects of HS-/H2S.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Nitric Oxide-Biology and Chemistry",
title = "Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study",
volume = "51",
pages = "19-23",
doi = "10.1016/j.niox.2015.09.008"
}

Bolic, B., Mijuskovic, A., Popovic-Bijelic, A., Nikolić-Kokić, A., Spasić, S., Blagojevic, D., Spasić, M.,& Spasojević, I.. (2015). Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study. in Nitric Oxide-Biology and Chemistry
Academic Press Inc Elsevier Science, San Diego., 51, 19-23.
https://doi.org/10.1016/j.niox.2015.09.008

Bolic B, Mijuskovic A, Popovic-Bijelic A, Nikolić-Kokić A, Spasić S, Blagojevic D, Spasić M, Spasojević I. Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study. in Nitric Oxide-Biology and Chemistry. 2015;51:19-23.
doi:10.1016/j.niox.2015.09.008 .

Bolic, Bojana, Mijuskovic, Ana, Popovic-Bijelic, Ana, Nikolić-Kokić, Aleksandra, Spasić, Snežana, Blagojevic, Dusko, Spasić, Mihajlo, Spasojević, Ivan, "Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study" in Nitric Oxide-Biology and Chemistry, 51 (2015):19-23,
https://doi.org/10.1016/j.niox.2015.09.008 . .

TY  - JOUR
AU  - Bolic, Bojana
AU  - Mijuskovic, Ana
AU  - Popovic-Bijelic, Ana
AU  - Nikolić-Kokić, Aleksandra
AU  - Spasić, Snežana
AU  - Blagojevic, Dusko
AU  - Spasić, Mihajlo
AU  - Spasojević, Ivan
PY  - 2015
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3187
AB  - Interactions of hydrogen sulfide (HS-/H2S), a reducing signaling species, with superoxide dimutases (SOD) are poorly understood. We applied low-T EPR spectroscopy to examine the effects of HS-/H2S and superoxide radical anion (O-2(-)) on metallocenters of FeSOD, MnSOD, and CuZnSOD. HS-/H2S did not affect FeSOD, whereas active centers of MnSOD and CuZnSOD were open to this agent. Cu2+ was reduced to Cu1+, while manganese appears to be released from MnSOD active center. Untreated and O-2(-) treated FeSOD and MnSOD predominantly show 5 d-electron systems, i.e. Fe3+ and Mn2+. Our study provides new details on the mechanisms of (patho)physiological effects of HS-/H2S.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Nitric Oxide-Biology and Chemistry
T1  - Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study
VL  - 51
SP  - 19
EP  - 23
DO  - 10.1016/j.niox.2015.09.008
ER  - 

@article{
author = "Bolic, Bojana and Mijuskovic, Ana and Popovic-Bijelic, Ana and Nikolić-Kokić, Aleksandra and Spasić, Snežana and Blagojevic, Dusko and Spasić, Mihajlo and Spasojević, Ivan",
year = "2015",
abstract = "Interactions of hydrogen sulfide (HS-/H2S), a reducing signaling species, with superoxide dimutases (SOD) are poorly understood. We applied low-T EPR spectroscopy to examine the effects of HS-/H2S and superoxide radical anion (O-2(-)) on metallocenters of FeSOD, MnSOD, and CuZnSOD. HS-/H2S did not affect FeSOD, whereas active centers of MnSOD and CuZnSOD were open to this agent. Cu2+ was reduced to Cu1+, while manganese appears to be released from MnSOD active center. Untreated and O-2(-) treated FeSOD and MnSOD predominantly show 5 d-electron systems, i.e. Fe3+ and Mn2+. Our study provides new details on the mechanisms of (patho)physiological effects of HS-/H2S.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Nitric Oxide-Biology and Chemistry",
title = "Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study",
volume = "51",
pages = "19-23",
doi = "10.1016/j.niox.2015.09.008"
}

Bolic, B., Mijuskovic, A., Popovic-Bijelic, A., Nikolić-Kokić, A., Spasić, S., Blagojevic, D., Spasić, M.,& Spasojević, I.. (2015). Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study. in Nitric Oxide-Biology and Chemistry
Academic Press Inc Elsevier Science, San Diego., 51, 19-23.
https://doi.org/10.1016/j.niox.2015.09.008

Bolic B, Mijuskovic A, Popovic-Bijelic A, Nikolić-Kokić A, Spasić S, Blagojevic D, Spasić M, Spasojević I. Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study. in Nitric Oxide-Biology and Chemistry. 2015;51:19-23.
doi:10.1016/j.niox.2015.09.008 .

Bolic, Bojana, Mijuskovic, Ana, Popovic-Bijelic, Ana, Nikolić-Kokić, Aleksandra, Spasić, Snežana, Blagojevic, Dusko, Spasić, Mihajlo, Spasojević, Ivan, "Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study" in Nitric Oxide-Biology and Chemistry, 51 (2015):19-23,
https://doi.org/10.1016/j.niox.2015.09.008 . .

TY  - JOUR
AU  - Lugonja, Nikoleta
AU  - Spasić, Snežana
AU  - Laugier, Olga B.
AU  - Nikolić-Kokić, Aleksandra
AU  - Spasojević, Ivan
AU  - Oreščanin-Dušić, Zorana
AU  - Vrvić, Miroslav
PY  - 2013
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1238
AB  - Objective: Early-onset and exclusive breast-feeding provides a significant health benefit to infants compared with infant formulas. The aim of this study was to compare mature breast milk with standard infant formulas by examining their effects on non-vascular smooth muscle contraction and their antioxidative properties. Methods: The pharmacologic effects of breast milk and formulas were examined using a model system of the rat uterine smooth muscle contraction. Electron paramagnetic resonance spin-trapping spectroscopy was used to compare the antioxidative capacities of breast milk (obtained in the ninth week of lactation) with commercial infant formulas against hydroxyl radical production in the Fenton reaction. The activities of superoxide dismutase, glutathione peroxidase, and the sulfhydryl group were determined in the breast milk and infant formulas. Results: In contrast to the infant formulas, breast milk exerted a relaxing effect on isolated non-vascular smooth muscle. In general, breast milk showed higher antioxidative activity compared with the infant formulas. In all samples, the generation of hydroxyl radicals led to the formation of carbon-centered and ascorbyl radicals. Conclusions: Human milk exerts direct pharmacologic relaxation effects and provides better antioxidant protection compared with infant formulas because of the presence of specific enzymatic components, such as human superoxide dismutase. We propose that these effects should be advantageous to an infant's gastrointestinal tract by supporting the normal work of the smooth musculature and maintaining redox homeostasis and may represent one of the mechanisms by which breast-feeding benefits health.
PB  - Elsevier Science Inc, New York
T2  - Nutrition
T1  - Differences in direct pharmacologic effects and antioxidative properties of mature breast milk and infant formulas
VL  - 29
IS  - 2
SP  - 431
EP  - 435
DO  - 10.1016/j.nut.2012.07.018
ER  - 

@article{
author = "Lugonja, Nikoleta and Spasić, Snežana and Laugier, Olga B. and Nikolić-Kokić, Aleksandra and Spasojević, Ivan and Oreščanin-Dušić, Zorana and Vrvić, Miroslav",
year = "2013",
abstract = "Objective: Early-onset and exclusive breast-feeding provides a significant health benefit to infants compared with infant formulas. The aim of this study was to compare mature breast milk with standard infant formulas by examining their effects on non-vascular smooth muscle contraction and their antioxidative properties. Methods: The pharmacologic effects of breast milk and formulas were examined using a model system of the rat uterine smooth muscle contraction. Electron paramagnetic resonance spin-trapping spectroscopy was used to compare the antioxidative capacities of breast milk (obtained in the ninth week of lactation) with commercial infant formulas against hydroxyl radical production in the Fenton reaction. The activities of superoxide dismutase, glutathione peroxidase, and the sulfhydryl group were determined in the breast milk and infant formulas. Results: In contrast to the infant formulas, breast milk exerted a relaxing effect on isolated non-vascular smooth muscle. In general, breast milk showed higher antioxidative activity compared with the infant formulas. In all samples, the generation of hydroxyl radicals led to the formation of carbon-centered and ascorbyl radicals. Conclusions: Human milk exerts direct pharmacologic relaxation effects and provides better antioxidant protection compared with infant formulas because of the presence of specific enzymatic components, such as human superoxide dismutase. We propose that these effects should be advantageous to an infant's gastrointestinal tract by supporting the normal work of the smooth musculature and maintaining redox homeostasis and may represent one of the mechanisms by which breast-feeding benefits health.",
publisher = "Elsevier Science Inc, New York",
journal = "Nutrition",
title = "Differences in direct pharmacologic effects and antioxidative properties of mature breast milk and infant formulas",
volume = "29",
number = "2",
pages = "431-435",
doi = "10.1016/j.nut.2012.07.018"
}

Lugonja, N., Spasić, S., Laugier, O. B., Nikolić-Kokić, A., Spasojević, I., Oreščanin-Dušić, Z.,& Vrvić, M.. (2013). Differences in direct pharmacologic effects and antioxidative properties of mature breast milk and infant formulas. in Nutrition
Elsevier Science Inc, New York., 29(2), 431-435.
https://doi.org/10.1016/j.nut.2012.07.018

Lugonja N, Spasić S, Laugier OB, Nikolić-Kokić A, Spasojević I, Oreščanin-Dušić Z, Vrvić M. Differences in direct pharmacologic effects and antioxidative properties of mature breast milk and infant formulas. in Nutrition. 2013;29(2):431-435.
doi:10.1016/j.nut.2012.07.018 .

Lugonja, Nikoleta, Spasić, Snežana, Laugier, Olga B., Nikolić-Kokić, Aleksandra, Spasojević, Ivan, Oreščanin-Dušić, Zorana, Vrvić, Miroslav, "Differences in direct pharmacologic effects and antioxidative properties of mature breast milk and infant formulas" in Nutrition, 29, no. 2 (2013):431-435,
https://doi.org/10.1016/j.nut.2012.07.018 . .

TY  - CONF
AU  - Spasić, Snežana
AU  - Lugonja, Nikoleta
AU  - Laugier, Olga B.
AU  - Oreščanin-Dušić, Zorana
AU  - Nikolić-Kokić, Aleksandra
AU  - Spasojević, Ivan
AU  - Vrvić, Miroslav
PY  - 2012
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5344
AB  - Poster presented at: 6th European Congress of Pharamcology “EPHAR 2012”
T1  - Differences in direct pharmacological effects and antioxidative properties of mature breast milk and an infant formula
UR  - https://hdl.handle.net/21.15107/rcub_cer_5344
ER  - 

@conference{
author = "Spasić, Snežana and Lugonja, Nikoleta and Laugier, Olga B. and Oreščanin-Dušić, Zorana and Nikolić-Kokić, Aleksandra and Spasojević, Ivan and Vrvić, Miroslav",
year = "2012",
abstract = "Poster presented at: 6th European Congress of Pharamcology “EPHAR 2012”",
title = "Differences in direct pharmacological effects and antioxidative properties of mature breast milk and an infant formula",
url = "https://hdl.handle.net/21.15107/rcub_cer_5344"
}

Spasić, S., Lugonja, N., Laugier, O. B., Oreščanin-Dušić, Z., Nikolić-Kokić, A., Spasojević, I.,& Vrvić, M.. (2012). Differences in direct pharmacological effects and antioxidative properties of mature breast milk and an infant formula. .
https://hdl.handle.net/21.15107/rcub_cer_5344

Spasić S, Lugonja N, Laugier OB, Oreščanin-Dušić Z, Nikolić-Kokić A, Spasojević I, Vrvić M. Differences in direct pharmacological effects and antioxidative properties of mature breast milk and an infant formula. 2012;.
https://hdl.handle.net/21.15107/rcub_cer_5344 .

Spasić, Snežana, Lugonja, Nikoleta, Laugier, Olga B., Oreščanin-Dušić, Zorana, Nikolić-Kokić, Aleksandra, Spasojević, Ivan, Vrvić, Miroslav, "Differences in direct pharmacological effects and antioxidative properties of mature breast milk and an infant formula" (2012),
https://hdl.handle.net/21.15107/rcub_cer_5344 .

TY  - CONF
AU  - Spasić, Snežana
AU  - Lugonja, Nikoleta
AU  - Laugier, Olga B.
AU  - Oreščanin-Dušić, Zorana
AU  - Nikolić-Kokić, Aleksandra
AU  - Spasojević, Ivan
AU  - Vrvić, Miroslav
PY  - 2012
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5343
AB  - Early onset and exclusive breastfeeding provides a significant health benefit to infants in comparison to infant formulas. There is groving evidence that presence of some specific protein components in mothers milk are responsible for observed significant health benefit in infants feed with mothers milk. The aim of this paper was to compare mature breast milk and a standard infant formula by examining their effects on smooth muscle contraction and their antioxidative properties. Electron paramagnetic resonance (EPR) spin-trapping spectroscopy was used to compare the antioxidative capacities of breast milk (obtained on the 9th week of lactation) with a commercial infant formula against hydroxyl radical production in the Fenton reaction. The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and their vitamin C and sulfhydryl group (–SH) contents were determined in the milks. Pharmacological research was performed on the isolated rat uterus. In contrast to the infant formula, breast milk exerted a
relaxing effect on isolated non-vascular smooth muscle. Using EPR and the Fenton reaction as a radical-generating system, we showed that breast milk possesses a three-fold higher antioxidative activity against the hydroxyl radical compared to the infant formula. In both samples, generation of the hydroxyl radical (•OH) led to the production of carbon-centered radicals. The ascorbyl radical was detected in breast milk but not in the infant formula. Human milk has direct pharmacological effects and provides better antioxidant protection than the infant formula due to the presence of specific protein components such as human SOD.
PB  - 6th European Congress of Pharamcology “EPHAR 2012”
C3  - 6th European Congress of Pharamcology “EPHAR 2012”, Granada, Spain, July 17-23 2012
T1  - Differences in direct pharmacological effects and antioxidative properties of mature breast milk and an infant formula
SP  - 351
UR  - https://hdl.handle.net/21.15107/rcub_cer_5343
ER  - 

@conference{
author = "Spasić, Snežana and Lugonja, Nikoleta and Laugier, Olga B. and Oreščanin-Dušić, Zorana and Nikolić-Kokić, Aleksandra and Spasojević, Ivan and Vrvić, Miroslav",
year = "2012",
abstract = "Early onset and exclusive breastfeeding provides a significant health benefit to infants in comparison to infant formulas. There is groving evidence that presence of some specific protein components in mothers milk are responsible for observed significant health benefit in infants feed with mothers milk. The aim of this paper was to compare mature breast milk and a standard infant formula by examining their effects on smooth muscle contraction and their antioxidative properties. Electron paramagnetic resonance (EPR) spin-trapping spectroscopy was used to compare the antioxidative capacities of breast milk (obtained on the 9th week of lactation) with a commercial infant formula against hydroxyl radical production in the Fenton reaction. The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and their vitamin C and sulfhydryl group (–SH) contents were determined in the milks. Pharmacological research was performed on the isolated rat uterus. In contrast to the infant formula, breast milk exerted a
relaxing effect on isolated non-vascular smooth muscle. Using EPR and the Fenton reaction as a radical-generating system, we showed that breast milk possesses a three-fold higher antioxidative activity against the hydroxyl radical compared to the infant formula. In both samples, generation of the hydroxyl radical (•OH) led to the production of carbon-centered radicals. The ascorbyl radical was detected in breast milk but not in the infant formula. Human milk has direct pharmacological effects and provides better antioxidant protection than the infant formula due to the presence of specific protein components such as human SOD.",
publisher = "6th European Congress of Pharamcology “EPHAR 2012”",
journal = "6th European Congress of Pharamcology “EPHAR 2012”, Granada, Spain, July 17-23 2012",
title = "Differences in direct pharmacological effects and antioxidative properties of mature breast milk and an infant formula",
pages = "351",
url = "https://hdl.handle.net/21.15107/rcub_cer_5343"
}

Spasić, S., Lugonja, N., Laugier, O. B., Oreščanin-Dušić, Z., Nikolić-Kokić, A., Spasojević, I.,& Vrvić, M.. (2012). Differences in direct pharmacological effects and antioxidative properties of mature breast milk and an infant formula. in 6th European Congress of Pharamcology “EPHAR 2012”, Granada, Spain, July 17-23 2012
6th European Congress of Pharamcology “EPHAR 2012”., 351.
https://hdl.handle.net/21.15107/rcub_cer_5343

Spasić S, Lugonja N, Laugier OB, Oreščanin-Dušić Z, Nikolić-Kokić A, Spasojević I, Vrvić M. Differences in direct pharmacological effects and antioxidative properties of mature breast milk and an infant formula. in 6th European Congress of Pharamcology “EPHAR 2012”, Granada, Spain, July 17-23 2012. 2012;:351.
https://hdl.handle.net/21.15107/rcub_cer_5343 .

Spasić, Snežana, Lugonja, Nikoleta, Laugier, Olga B., Oreščanin-Dušić, Zorana, Nikolić-Kokić, Aleksandra, Spasojević, Ivan, Vrvić, Miroslav, "Differences in direct pharmacological effects and antioxidative properties of mature breast milk and an infant formula" in 6th European Congress of Pharamcology “EPHAR 2012”, Granada, Spain, July 17-23 2012 (2012):351,
https://hdl.handle.net/21.15107/rcub_cer_5343 .

TY  - JOUR
AU  - Ignjatović, Đurđica S.
AU  - Milutinovic, Danijela Vojnovic
AU  - Nikolić-Kokić, Aleksandra
AU  - Slavic, Marija
AU  - Andrić, Deana
AU  - Tomić, Mirko
AU  - Kostić Rajačić, Slađana
PY  - 2012
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/955
AB  - A group of sixteen arylpiperazines had been previously synthesized and evaluated for atypical antipsychotic activity. Here we examined these compounds for their neuroprotective capacity. The affinity and agonist/antagonist action of the arylpiperazines at dopamine hD(2S) receptors were determined in vitro on membranes from stably transfected CHO-hD(2S) cell line. The assays for cell viability and antioxidative capacity (total glutathione and total superoxide dismutase activity), amount of nitric oxide and superoxide radicals, as well as influence on prosurvival pathways (Akt and ERK), were performed on the human neuroblastoma cell line SH-SY5Y. Cell death was induced by oxidative or nitrosative stress, or by growing cells in the medium deprived of serum. Only four of the arylpiperazines exhibited notable neuroprotection against cell death induced by sodium nitroprusside. Two of these arylpiperazines induced elevations of pAkt, while two other compounds reduced the levels of pErk, whereas these actions are considered to support the cell survival. The benzimidazole heteroaryl-group, that mimics catechol moiety of the dopamine molecule, might be the prerequisite structure for the neuroprotective action of these ligands. It is postulated that neuroprotection was acquired also by elevation of endogenous glutathione or total superoxide dismutase activity.
PB  - Elsevier
T2  - European Journal of Pharmacology
T1  - The mechanisms responsible for neuroprotective capacity of arylpiperazine dopaminergic ligands against cell death induced by sodium nitroprusside
VL  - 683
IS  - 1-3
SP  - 93
EP  - 100
DO  - 10.1016/j.ejphar.2012.03.011
ER  - 

@article{
author = "Ignjatović, Đurđica S. and Milutinovic, Danijela Vojnovic and Nikolić-Kokić, Aleksandra and Slavic, Marija and Andrić, Deana and Tomić, Mirko and Kostić Rajačić, Slađana",
year = "2012",
abstract = "A group of sixteen arylpiperazines had been previously synthesized and evaluated for atypical antipsychotic activity. Here we examined these compounds for their neuroprotective capacity. The affinity and agonist/antagonist action of the arylpiperazines at dopamine hD(2S) receptors were determined in vitro on membranes from stably transfected CHO-hD(2S) cell line. The assays for cell viability and antioxidative capacity (total glutathione and total superoxide dismutase activity), amount of nitric oxide and superoxide radicals, as well as influence on prosurvival pathways (Akt and ERK), were performed on the human neuroblastoma cell line SH-SY5Y. Cell death was induced by oxidative or nitrosative stress, or by growing cells in the medium deprived of serum. Only four of the arylpiperazines exhibited notable neuroprotection against cell death induced by sodium nitroprusside. Two of these arylpiperazines induced elevations of pAkt, while two other compounds reduced the levels of pErk, whereas these actions are considered to support the cell survival. The benzimidazole heteroaryl-group, that mimics catechol moiety of the dopamine molecule, might be the prerequisite structure for the neuroprotective action of these ligands. It is postulated that neuroprotection was acquired also by elevation of endogenous glutathione or total superoxide dismutase activity.",
publisher = "Elsevier",
journal = "European Journal of Pharmacology",
title = "The mechanisms responsible for neuroprotective capacity of arylpiperazine dopaminergic ligands against cell death induced by sodium nitroprusside",
volume = "683",
number = "1-3",
pages = "93-100",
doi = "10.1016/j.ejphar.2012.03.011"
}

Ignjatović, Đ. S., Milutinovic, D. V., Nikolić-Kokić, A., Slavic, M., Andrić, D., Tomić, M.,& Kostić Rajačić, S.. (2012). The mechanisms responsible for neuroprotective capacity of arylpiperazine dopaminergic ligands against cell death induced by sodium nitroprusside. in European Journal of Pharmacology
Elsevier., 683(1-3), 93-100.
https://doi.org/10.1016/j.ejphar.2012.03.011

Ignjatović ĐS, Milutinovic DV, Nikolić-Kokić A, Slavic M, Andrić D, Tomić M, Kostić Rajačić S. The mechanisms responsible for neuroprotective capacity of arylpiperazine dopaminergic ligands against cell death induced by sodium nitroprusside. in European Journal of Pharmacology. 2012;683(1-3):93-100.
doi:10.1016/j.ejphar.2012.03.011 .

Ignjatović, Đurđica S., Milutinovic, Danijela Vojnovic, Nikolić-Kokić, Aleksandra, Slavic, Marija, Andrić, Deana, Tomić, Mirko, Kostić Rajačić, Slađana, "The mechanisms responsible for neuroprotective capacity of arylpiperazine dopaminergic ligands against cell death induced by sodium nitroprusside" in European Journal of Pharmacology, 683, no. 1-3 (2012):93-100,
https://doi.org/10.1016/j.ejphar.2012.03.011 . .