TY  - JOUR
AU  - Lugonja, Nikoleta
AU  - Marinković, Vesna
AU  - Miličić, Biljana
AU  - Avdalović, Jelena
AU  - Vrvić, Miroslav
AU  - Spasić, Snežana
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7408
AB  - Freeze storage and pasteurization of human milk are common treatments in milk banks. Thermal treatment changes the quality of milk for preterm infants’ nutrition, and the aim of this paper was to examine the nutritional profile and antioxidant potential of preterm human milk after storage and pasteurization, and also after supplementation with fortifier. The effects of storage processes were estimated on mature preterm milk of 30 breastfeeding women. Total proteins, lipids and lactose were determined after thermal processing and supplementation of mature preterm milk with fortifier. The antioxidant capacity was determined using the ferric reducing antioxidant potential method and lipid peroxidation inhibition assay. Protein concentration decreased after frozen storage and pasteurization (p<0.05). Pasteurization further reduced the lipid concentration after freezing. The ferric reducing antioxidant potential decreased after thermal treatments (p<0.05). Supplementation of mature milk with fortifier increased the concentration of proteins, lipids and lactose. Our findings demonstrated that storage and pasteurization processes affect the basic nutritional composition and antioxidant capacity of preterm human milk. In order to ensure adequate nutrition for preterm infants with preterm human milk, supplementation, especially with high concentrations of proteins and lipids, is necessary after thermal treatments.
PB  - Association of the Chemical Engineers of Serbia
T2  - Chemical Industry and Chemical Engineering Quarterly
T1  - Effect of storage process on nutritive properties of preterm human milk
VL  - 29
IS  - 2
SP  - 141
EP  - 148
DO  - 10.2298/CICEQ220117021L
ER  - 

@article{
author = "Lugonja, Nikoleta and Marinković, Vesna and Miličić, Biljana and Avdalović, Jelena and Vrvić, Miroslav and Spasić, Snežana",
year = "2023",
abstract = "Freeze storage and pasteurization of human milk are common treatments in milk banks. Thermal treatment changes the quality of milk for preterm infants’ nutrition, and the aim of this paper was to examine the nutritional profile and antioxidant potential of preterm human milk after storage and pasteurization, and also after supplementation with fortifier. The effects of storage processes were estimated on mature preterm milk of 30 breastfeeding women. Total proteins, lipids and lactose were determined after thermal processing and supplementation of mature preterm milk with fortifier. The antioxidant capacity was determined using the ferric reducing antioxidant potential method and lipid peroxidation inhibition assay. Protein concentration decreased after frozen storage and pasteurization (p<0.05). Pasteurization further reduced the lipid concentration after freezing. The ferric reducing antioxidant potential decreased after thermal treatments (p<0.05). Supplementation of mature milk with fortifier increased the concentration of proteins, lipids and lactose. Our findings demonstrated that storage and pasteurization processes affect the basic nutritional composition and antioxidant capacity of preterm human milk. In order to ensure adequate nutrition for preterm infants with preterm human milk, supplementation, especially with high concentrations of proteins and lipids, is necessary after thermal treatments.",
publisher = "Association of the Chemical Engineers of Serbia",
journal = "Chemical Industry and Chemical Engineering Quarterly",
title = "Effect of storage process on nutritive properties of preterm human milk",
volume = "29",
number = "2",
pages = "141-148",
doi = "10.2298/CICEQ220117021L"
}

Lugonja, N., Marinković, V., Miličić, B., Avdalović, J., Vrvić, M.,& Spasić, S.. (2023). Effect of storage process on nutritive properties of preterm human milk. in Chemical Industry and Chemical Engineering Quarterly
Association of the Chemical Engineers of Serbia., 29(2), 141-148.
https://doi.org/10.2298/CICEQ220117021L

Lugonja N, Marinković V, Miličić B, Avdalović J, Vrvić M, Spasić S. Effect of storage process on nutritive properties of preterm human milk. in Chemical Industry and Chemical Engineering Quarterly. 2023;29(2):141-148.
doi:10.2298/CICEQ220117021L .

Lugonja, Nikoleta, Marinković, Vesna, Miličić, Biljana, Avdalović, Jelena, Vrvić, Miroslav, Spasić, Snežana, "Effect of storage process on nutritive properties of preterm human milk" in Chemical Industry and Chemical Engineering Quarterly, 29, no. 2 (2023):141-148,
https://doi.org/10.2298/CICEQ220117021L . .

TY  - JOUR
AU  - Lugonja, Nikoleta
AU  - Marinković, Vesna
AU  - Miličić, Biljana
AU  - Avdalović, Jelena
AU  - Vrvić, Miroslav
AU  - Spasić, Snežana
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5608
AB  - Freeze storage and pasteurization of human milk are common treatments in milk banks. Thermal treatment changes the quality of milk for preterm infants’ nutrition, and the aim of this paper was to examine the nutritional profile and antioxidant potential of preterm human milk after storage and pasteurization, and also after supplementation with fortifier. The effects of storage processes were estimated on mature preterm milk of 30 breastfeeding women. Total proteins, lipids and lactose were determined after thermal processing and supplementation of mature preterm milk with fortifier. The antioxidant capacity was determined using the ferric reducing antioxidant potential method and lipid peroxidation inhibition assay. Protein concentration decreased after frozen storage and pasteurization (p<0.05). Pasteurization further reduced the lipid concentration after freezing. The ferric reducing antioxidant potential decreased after thermal treatments (p<0.05). Supplementation of mature milk with fortifier increased the concentration of proteins, lipids and lactose. Our findings demonstrated that storage and pasteurization processes affect the basic nutritional composition and antioxidant capacity of preterm human milk. In order to ensure adequate nutrition for preterm infants with preterm human milk, supplementation, especially with high concentrations of proteins and lipids, is necessary after thermal treatments.
PB  - Association of the Chemical Engineers of Serbia
T2  - Chemical Industry and Chemical Engineering Quarterly
T1  - Effect of storage process on nutritive properties of preterm human milk
VL  - 29
IS  - 2
SP  - 141
EP  - 148
DO  - 10.2298/CICEQ220117021L
ER  - 

@article{
author = "Lugonja, Nikoleta and Marinković, Vesna and Miličić, Biljana and Avdalović, Jelena and Vrvić, Miroslav and Spasić, Snežana",
year = "2023",
abstract = "Freeze storage and pasteurization of human milk are common treatments in milk banks. Thermal treatment changes the quality of milk for preterm infants’ nutrition, and the aim of this paper was to examine the nutritional profile and antioxidant potential of preterm human milk after storage and pasteurization, and also after supplementation with fortifier. The effects of storage processes were estimated on mature preterm milk of 30 breastfeeding women. Total proteins, lipids and lactose were determined after thermal processing and supplementation of mature preterm milk with fortifier. The antioxidant capacity was determined using the ferric reducing antioxidant potential method and lipid peroxidation inhibition assay. Protein concentration decreased after frozen storage and pasteurization (p<0.05). Pasteurization further reduced the lipid concentration after freezing. The ferric reducing antioxidant potential decreased after thermal treatments (p<0.05). Supplementation of mature milk with fortifier increased the concentration of proteins, lipids and lactose. Our findings demonstrated that storage and pasteurization processes affect the basic nutritional composition and antioxidant capacity of preterm human milk. In order to ensure adequate nutrition for preterm infants with preterm human milk, supplementation, especially with high concentrations of proteins and lipids, is necessary after thermal treatments.",
publisher = "Association of the Chemical Engineers of Serbia",
journal = "Chemical Industry and Chemical Engineering Quarterly",
title = "Effect of storage process on nutritive properties of preterm human milk",
volume = "29",
number = "2",
pages = "141-148",
doi = "10.2298/CICEQ220117021L"
}

Lugonja, N., Marinković, V., Miličić, B., Avdalović, J., Vrvić, M.,& Spasić, S.. (2023). Effect of storage process on nutritive properties of preterm human milk. in Chemical Industry and Chemical Engineering Quarterly
Association of the Chemical Engineers of Serbia., 29(2), 141-148.
https://doi.org/10.2298/CICEQ220117021L

Lugonja N, Marinković V, Miličić B, Avdalović J, Vrvić M, Spasić S. Effect of storage process on nutritive properties of preterm human milk. in Chemical Industry and Chemical Engineering Quarterly. 2023;29(2):141-148.
doi:10.2298/CICEQ220117021L .

Lugonja, Nikoleta, Marinković, Vesna, Miličić, Biljana, Avdalović, Jelena, Vrvić, Miroslav, Spasić, Snežana, "Effect of storage process on nutritive properties of preterm human milk" in Chemical Industry and Chemical Engineering Quarterly, 29, no. 2 (2023):141-148,
https://doi.org/10.2298/CICEQ220117021L . .

TY  - JOUR
AU  - Lugonja, Nikoleta
AU  - Gorjanović, Stanislava Ž.
AU  - Pastor, Ferenc T.
AU  - Marinković, Vesna
AU  - Miličić, Biljana
AU  - Vrvić, Miroslav
AU  - Spasić, Snežana
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4574
AB  - Antioxidants present in infant food contribute to its nutritive value and provide protection from oxidative damage. Here, a direct current polarographic method, based on a decrease of anodic current from the hydroxoperhydroxo-mercury(II) complex (HPMC) formed at a mercury dropping electrode at its potential of dissolution, was used to determine the total antioxidant capacity (TAC) of infant formulas, human milk collected from mothers of full-term and preterm infants, human milk supplemented with fortifier, and cow milk. TAC of milk samples and infant formula correlated with protein content measured by the Bradford method (Pearson correlation coefficient 0.605, p<0.001), while TAC of human milk correlated with content of free thiol groups measured by the Ellman method (0.779, p<0.001). The results obtained showed that, in contrast to the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, HPMC enables specific insight into the quality of infant milk food which reflects the amounts of free thiol groups and protein content. The significant contribution of milk proteins and the most prevalent amino acids, particularly cysteine, arginine, and phenylalanine, to TAC was shown. Thus, the HPMC method might be useful in monitoring the quality of infant formulas and human milk for preterm infants before and after supplementation with fortifier.
PB  - Springer
T2  - Food Analytical Methods
T1  - Antioxidant Capacity and Quality of Human Milk and Infant Formula Determined by Direct Current Polarography
DO  - 10.1007/s12161-021-02030-3
ER  - 

@article{
author = "Lugonja, Nikoleta and Gorjanović, Stanislava Ž. and Pastor, Ferenc T. and Marinković, Vesna and Miličić, Biljana and Vrvić, Miroslav and Spasić, Snežana",
year = "2021",
abstract = "Antioxidants present in infant food contribute to its nutritive value and provide protection from oxidative damage. Here, a direct current polarographic method, based on a decrease of anodic current from the hydroxoperhydroxo-mercury(II) complex (HPMC) formed at a mercury dropping electrode at its potential of dissolution, was used to determine the total antioxidant capacity (TAC) of infant formulas, human milk collected from mothers of full-term and preterm infants, human milk supplemented with fortifier, and cow milk. TAC of milk samples and infant formula correlated with protein content measured by the Bradford method (Pearson correlation coefficient 0.605, p<0.001), while TAC of human milk correlated with content of free thiol groups measured by the Ellman method (0.779, p<0.001). The results obtained showed that, in contrast to the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, HPMC enables specific insight into the quality of infant milk food which reflects the amounts of free thiol groups and protein content. The significant contribution of milk proteins and the most prevalent amino acids, particularly cysteine, arginine, and phenylalanine, to TAC was shown. Thus, the HPMC method might be useful in monitoring the quality of infant formulas and human milk for preterm infants before and after supplementation with fortifier.",
publisher = "Springer",
journal = "Food Analytical Methods",
title = "Antioxidant Capacity and Quality of Human Milk and Infant Formula Determined by Direct Current Polarography",
doi = "10.1007/s12161-021-02030-3"
}

Lugonja, N., Gorjanović, S. Ž., Pastor, F. T., Marinković, V., Miličić, B., Vrvić, M.,& Spasić, S.. (2021). Antioxidant Capacity and Quality of Human Milk and Infant Formula Determined by Direct Current Polarography. in Food Analytical Methods
Springer..
https://doi.org/10.1007/s12161-021-02030-3

Lugonja N, Gorjanović SŽ, Pastor FT, Marinković V, Miličić B, Vrvić M, Spasić S. Antioxidant Capacity and Quality of Human Milk and Infant Formula Determined by Direct Current Polarography. in Food Analytical Methods. 2021;.
doi:10.1007/s12161-021-02030-3 .

Lugonja, Nikoleta, Gorjanović, Stanislava Ž., Pastor, Ferenc T., Marinković, Vesna, Miličić, Biljana, Vrvić, Miroslav, Spasić, Snežana, "Antioxidant Capacity and Quality of Human Milk and Infant Formula Determined by Direct Current Polarography" in Food Analytical Methods (2021),
https://doi.org/10.1007/s12161-021-02030-3 . .

TY  - JOUR
AU  - Lugonja, Nikoleta
AU  - Stanković, Dalibor
AU  - Miličić, Biljana
AU  - Spasić, Snežana
AU  - Marinković, Vesna
AU  - Vrvić, Miroslav
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2484
AB  - The electrochemical techniques were used to determine the total antioxidant capacity of breast milks and the results were compared with a commonly used spectrophotometric (2,2-diphenyl-1-picrylhydrazyl, DPPH) method. Breast milk from mothers of preterm infants was monitored in three lactation phases and after storage of expressed milk by monitoring changes in the total antioxidant capacity over a two year period. Statistical analysis showed there was no significant difference between the ability of the three methods to detect changes in breast milk after storage. Either of the electrochemical techniques studied could be successfully used to replace the time-consuming spectrophotometric method and can be applied to clinical trials.
PB  - Elsevier
T2  - Food Chemistry
T1  - Electrochemical monitoring of the breast milk quality
VL  - 240
SP  - 567
EP  - 572
DO  - 10.1016/j.foodchem.2017.07.164
ER  - 

@article{
author = "Lugonja, Nikoleta and Stanković, Dalibor and Miličić, Biljana and Spasić, Snežana and Marinković, Vesna and Vrvić, Miroslav",
year = "2018",
abstract = "The electrochemical techniques were used to determine the total antioxidant capacity of breast milks and the results were compared with a commonly used spectrophotometric (2,2-diphenyl-1-picrylhydrazyl, DPPH) method. Breast milk from mothers of preterm infants was monitored in three lactation phases and after storage of expressed milk by monitoring changes in the total antioxidant capacity over a two year period. Statistical analysis showed there was no significant difference between the ability of the three methods to detect changes in breast milk after storage. Either of the electrochemical techniques studied could be successfully used to replace the time-consuming spectrophotometric method and can be applied to clinical trials.",
publisher = "Elsevier",
journal = "Food Chemistry",
title = "Electrochemical monitoring of the breast milk quality",
volume = "240",
pages = "567-572",
doi = "10.1016/j.foodchem.2017.07.164"
}

Lugonja, N., Stanković, D., Miličić, B., Spasić, S., Marinković, V.,& Vrvić, M.. (2018). Electrochemical monitoring of the breast milk quality. in Food Chemistry
Elsevier., 240, 567-572.
https://doi.org/10.1016/j.foodchem.2017.07.164

Lugonja N, Stanković D, Miličić B, Spasić S, Marinković V, Vrvić M. Electrochemical monitoring of the breast milk quality. in Food Chemistry. 2018;240:567-572.
doi:10.1016/j.foodchem.2017.07.164 .

Lugonja, Nikoleta, Stanković, Dalibor, Miličić, Biljana, Spasić, Snežana, Marinković, Vesna, Vrvić, Miroslav, "Electrochemical monitoring of the breast milk quality" in Food Chemistry, 240 (2018):567-572,
https://doi.org/10.1016/j.foodchem.2017.07.164 . .