TY  - JOUR
AU  - Mandić, Ljuba
AU  - Stojanović, Srđan
AU  - Čolović, Ana
AU  - Milin, Nenad
PY  - 2000
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3889
AB  - Određivanje sadržaja glikozilovanih proteina je važno za otkrivanje i kontrolu stanja dugotrajne hiperglikemijc u dijabetesu. Cl ovom radu određivan je sadržaj glukoze vezane za proteine seruma kod obolelih od dijabetesa, spektrolotometrijskim metodama s fenol-sumpornom kiselinom (PS) i s tiobarbiturnom kiselinom (TBA). Vrednosti vezane glukoze dobijene s PS reagensom (80,6 ± 22,8 gmol glukoze/g proteina) su bile znatno više od do-bijenih s TBA metodom (3,71 ± 1,34 pmol glukoze/g proteina). Analiza faktora, koji bi mogli dovesti do ove značajne razlike, je pokazala da su više vrednosti dobijene PS metodom posledica dodatne dehidratacije ugljenih hidrata oslobođenih u toku hidrolize glikozilovanih proteina. Do dehidratacije dolazi pri izvođenju bojene reakcije s fenol-sumpornom kiselinom. Zaključeno je da, iako se osetljivost obe metode za fruktozu kao standard ne razlikuje značajno, TBA metoda je pouzdanija od PS metode i preporučuje se za kliničku praksu.
AB  - The estimation of glycosylated proteins is useful in the detection and control of long-standing hyperglycemic conditions. In this paper the content of protein-bound glucose was determined in diabetics by spectrophotometric method with phenol-sulphuric acid (PS) and with thiobarbituric acid (TBA). The amounts of protein-bound glucose obtained with the PS reagent (80.6 ± 22.8 mmol glucose/g protein) were always considerably higher than those obtained by TBA method (3.71 ± 1.34 μmol glucose/g protein). The analysis was made of parameters that might have led to such significant difference. It was shown that in the reaction with PS reagent there was an additional dehydration of carbohydrate released during the hydrolysis of glycosylated proteins. The additional dehydration was due to concentrated sulphuric acid which at higher temperature influenced chromogen formation. On the basis of the results obtained it was concluded that, although the sensitivity of the both methods for standard fructose was comparable, TBA method was more reliable than PS method. Therefore, it is recommended in clinical practice.
PB  - Društvo medicinskih biohemičara Srbije i Crne Gore i Farmaceutski fakultet, Beograd
T2  - Jugoslovenska medicinska biohemija - Yugoslav Medical Biochemistry
T1  - Odredivanje glikozilovanih proteina seruma: poredenje metoda sa tiobarbiturnom kiselinom i fenol-sumpornom kiselinom
T1  - Determination of serum glycosylated proteins: Comparison of thiobarbituric acid and phenol-sulphuric acid assays
VL  - 19
IS  - 4
SP  - 411
EP  - 416
UR  - https://hdl.handle.net/21.15107/rcub_cherry_445
ER  - 

@article{
author = "Mandić, Ljuba and Stojanović, Srđan and Čolović, Ana and Milin, Nenad",
year = "2000",
abstract = "Određivanje sadržaja glikozilovanih proteina je važno za otkrivanje i kontrolu stanja dugotrajne hiperglikemijc u dijabetesu. Cl ovom radu određivan je sadržaj glukoze vezane za proteine seruma kod obolelih od dijabetesa, spektrolotometrijskim metodama s fenol-sumpornom kiselinom (PS) i s tiobarbiturnom kiselinom (TBA). Vrednosti vezane glukoze dobijene s PS reagensom (80,6 ± 22,8 gmol glukoze/g proteina) su bile znatno više od do-bijenih s TBA metodom (3,71 ± 1,34 pmol glukoze/g proteina). Analiza faktora, koji bi mogli dovesti do ove značajne razlike, je pokazala da su više vrednosti dobijene PS metodom posledica dodatne dehidratacije ugljenih hidrata oslobođenih u toku hidrolize glikozilovanih proteina. Do dehidratacije dolazi pri izvođenju bojene reakcije s fenol-sumpornom kiselinom. Zaključeno je da, iako se osetljivost obe metode za fruktozu kao standard ne razlikuje značajno, TBA metoda je pouzdanija od PS metode i preporučuje se za kliničku praksu., The estimation of glycosylated proteins is useful in the detection and control of long-standing hyperglycemic conditions. In this paper the content of protein-bound glucose was determined in diabetics by spectrophotometric method with phenol-sulphuric acid (PS) and with thiobarbituric acid (TBA). The amounts of protein-bound glucose obtained with the PS reagent (80.6 ± 22.8 mmol glucose/g protein) were always considerably higher than those obtained by TBA method (3.71 ± 1.34 μmol glucose/g protein). The analysis was made of parameters that might have led to such significant difference. It was shown that in the reaction with PS reagent there was an additional dehydration of carbohydrate released during the hydrolysis of glycosylated proteins. The additional dehydration was due to concentrated sulphuric acid which at higher temperature influenced chromogen formation. On the basis of the results obtained it was concluded that, although the sensitivity of the both methods for standard fructose was comparable, TBA method was more reliable than PS method. Therefore, it is recommended in clinical practice.",
publisher = "Društvo medicinskih biohemičara Srbije i Crne Gore i Farmaceutski fakultet, Beograd",
journal = "Jugoslovenska medicinska biohemija - Yugoslav Medical Biochemistry",
title = "Odredivanje glikozilovanih proteina seruma: poredenje metoda sa tiobarbiturnom kiselinom i fenol-sumpornom kiselinom, Determination of serum glycosylated proteins: Comparison of thiobarbituric acid and phenol-sulphuric acid assays",
volume = "19",
number = "4",
pages = "411-416",
url = "https://hdl.handle.net/21.15107/rcub_cherry_445"
}

Mandić, L., Stojanović, S., Čolović, A.,& Milin, N.. (2000). Odredivanje glikozilovanih proteina seruma: poredenje metoda sa tiobarbiturnom kiselinom i fenol-sumpornom kiselinom. in Jugoslovenska medicinska biohemija - Yugoslav Medical Biochemistry
Društvo medicinskih biohemičara Srbije i Crne Gore i Farmaceutski fakultet, Beograd., 19(4), 411-416.
https://hdl.handle.net/21.15107/rcub_cherry_445

Mandić L, Stojanović S, Čolović A, Milin N. Odredivanje glikozilovanih proteina seruma: poredenje metoda sa tiobarbiturnom kiselinom i fenol-sumpornom kiselinom. in Jugoslovenska medicinska biohemija - Yugoslav Medical Biochemistry. 2000;19(4):411-416.
https://hdl.handle.net/21.15107/rcub_cherry_445 .

Mandić, Ljuba, Stojanović, Srđan, Čolović, Ana, Milin, Nenad, "Odredivanje glikozilovanih proteina seruma: poredenje metoda sa tiobarbiturnom kiselinom i fenol-sumpornom kiselinom" in Jugoslovenska medicinska biohemija - Yugoslav Medical Biochemistry, 19, no. 4 (2000):411-416,
https://hdl.handle.net/21.15107/rcub_cherry_445 .

TY  - CONF
AU  - Mandić, Ljuba
AU  - Stojanović, Srđan
AU  - Milin, Nenad
AU  - Čolović, Ana
PY  - 1998
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6539
AB  - In this work a new spectrophotometric method for determination of nonenzymatically scrum protein-bound carbohydrates by phenol-sulfuric acid [5] as reagent, was used. All relevant parameters as the conditions of hydrolysis of ketoamine bond between glucose and protein, and formation 5-hydroxymethylfurfuraI (5-HMF) from glucose, as well as non specific influences on the reaction 5-HMF and •phenol-sulfuric acid, were investigated. Thus the determination of protein-bound carbohydrates by phenol-sulfuric acid was found more sensitive than spectrophotometric one which use thiobarbituric acid as reagent. More significant correlation with HbAlc content, determined by ion-exchange chromatography, was found. The non-specific influences on the determination was not found. Therefore, the determination of protein-bound carbohydrates by phenol­ sulfuric acid can serve as sensitive indicator of the degree hyperglycemia in diabetes.
PB  - Association of Greek Chemists
C3  - Book of abstracts - 1st International Conference of the Chemical Societies of the South-East European Countries, June 1-4. 1999, Halkidiki, Greece
T1  - Determination of nonenzymatically protein-bound carbohydrates in serum by phenol-sulfuric acid
SP  - PO407
EP  - PO407
UR  - https://hdl.handle.net/21.15107/rcub_cer_6539
ER  - 

@conference{
author = "Mandić, Ljuba and Stojanović, Srđan and Milin, Nenad and Čolović, Ana",
year = "1998",
abstract = "In this work a new spectrophotometric method for determination of nonenzymatically scrum protein-bound carbohydrates by phenol-sulfuric acid [5] as reagent, was used. All relevant parameters as the conditions of hydrolysis of ketoamine bond between glucose and protein, and formation 5-hydroxymethylfurfuraI (5-HMF) from glucose, as well as non specific influences on the reaction 5-HMF and •phenol-sulfuric acid, were investigated. Thus the determination of protein-bound carbohydrates by phenol-sulfuric acid was found more sensitive than spectrophotometric one which use thiobarbituric acid as reagent. More significant correlation with HbAlc content, determined by ion-exchange chromatography, was found. The non-specific influences on the determination was not found. Therefore, the determination of protein-bound carbohydrates by phenol­ sulfuric acid can serve as sensitive indicator of the degree hyperglycemia in diabetes.",
publisher = "Association of Greek Chemists",
journal = "Book of abstracts - 1st International Conference of the Chemical Societies of the South-East European Countries, June 1-4. 1999, Halkidiki, Greece",
title = "Determination of nonenzymatically protein-bound carbohydrates in serum by phenol-sulfuric acid",
pages = "PO407-PO407",
url = "https://hdl.handle.net/21.15107/rcub_cer_6539"
}

Mandić, L., Stojanović, S., Milin, N.,& Čolović, A.. (1998). Determination of nonenzymatically protein-bound carbohydrates in serum by phenol-sulfuric acid. in Book of abstracts - 1st International Conference of the Chemical Societies of the South-East European Countries, June 1-4. 1999, Halkidiki, Greece
Association of Greek Chemists., PO407-PO407.
https://hdl.handle.net/21.15107/rcub_cer_6539

Mandić L, Stojanović S, Milin N, Čolović A. Determination of nonenzymatically protein-bound carbohydrates in serum by phenol-sulfuric acid. in Book of abstracts - 1st International Conference of the Chemical Societies of the South-East European Countries, June 1-4. 1999, Halkidiki, Greece. 1998;:PO407-PO407.
https://hdl.handle.net/21.15107/rcub_cer_6539 .

Mandić, Ljuba, Stojanović, Srđan, Milin, Nenad, Čolović, Ana, "Determination of nonenzymatically protein-bound carbohydrates in serum by phenol-sulfuric acid" in Book of abstracts - 1st International Conference of the Chemical Societies of the South-East European Countries, June 1-4. 1999, Halkidiki, Greece (1998):PO407-PO407,
https://hdl.handle.net/21.15107/rcub_cer_6539 .

TY  - CONF
AU  - Stojanović, Srđan
AU  - Milin, Nenad
AU  - Đurđić, Vidosava
PY  - 1992
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6590
AB  - Stepen glikemije može biti određen analizom sadržaja glukoze ili ugljenih hidrata ne-enzimski veza­ nih za proteine krvi. Već se duže vremena koristi određivanje ugljenih hidrata vezanih za hemoglobulin ili proteine seruma. Za ova određivanja koriste se, pored ostalih, najčešće spektrofotometrijske metode sa tiobarbiturnom kiselinom kao reagensom za izazi­ vanje boja. Međutim ova metoda se pokazala relativno neosetljivom i nereproduktivnom. Cl ovome radu data je nova spektrofotometrijska metoda za određivanje ne-enzimski vezanih ugljenih hidrata za proteine seru­ ma, koja se zasniva na reakciji sa fenol-sumpornom kiselinom. Ispitani su i značajni parametri za primenu metode, kao što su uslovi izvođenja hidrolize, građenja hidroksimetil-furfurala i hromofore, kao i mogući ne­ specifični uticaj na reakciju. Metoda se pokazala više osetljivom od spektrofotometrijskog određivanja sa tiobarbituralnom kiselinom. Nađena je bolja korelacija sa sadržajem HbAic određenim jonoizmenjivačkom hromatografijom, povećanje sadržaja ugljenih hidrata kod dijabetičara bilo je značajnije. Pored toga nije ustanovljen nespecifičan uticaj drugoih supstanci na određivanje.
AB  - The degree of glycemic control may be performed by analysis of serum glycose content, as well as by determination of non-enzymatically protein-bound carbohydrates in blood. Thus, the determination of hemoglobin and serum protein-bound carbohydrates are used in clinical praxis. These determinations were performed commonly by spectrophotometric method based on thiobarbituric acid as reagent. But this method is not sensitive and reproductive enough. In this work a new spectrophotometric method for determination of non-enzymatically serum-protein bound carbohydrates was proposed. In the method phenol-sulfuric acid as reagent was used. All relevant parameters were investigated, as the conditions of hydrolysis and formation hydroxymethyl-furfural, as well as non-specific influences on the reaction. The method was found more sensitive than spectrophoto-metric one with use thiobarbituric acid as reagent. More significant correlation with HbAtc contents, determined by ion-exchange chromatography, was found. The elevation of protein-bound carbohydrates in diabetics was more significant and the non-specific influences on the determination was not found.
PB  - Društvo medicinskih biohemičara Jugoslavije
C3  - Jugoslovenska medicinska biohemija / Yugoslav Medical Biochemistry
T1  - Spektrofotometrijsko određivanje ugljenih hidrata neenzimski vezanih za proteine seruma
T1  - A spectrophotometric determination of a non-enzymatically protein-bound carbohydrates in blood
VL  - 11
IS  - 3-4
SP  - 120
EP  - 120
UR  - https://hdl.handle.net/21.15107/rcub_cer_6590
ER  - 

@conference{
author = "Stojanović, Srđan and Milin, Nenad and Đurđić, Vidosava",
year = "1992",
abstract = "Stepen glikemije može biti određen analizom sadržaja glukoze ili ugljenih hidrata ne-enzimski veza­ nih za proteine krvi. Već se duže vremena koristi određivanje ugljenih hidrata vezanih za hemoglobulin ili proteine seruma. Za ova određivanja koriste se, pored ostalih, najčešće spektrofotometrijske metode sa tiobarbiturnom kiselinom kao reagensom za izazi­ vanje boja. Međutim ova metoda se pokazala relativno neosetljivom i nereproduktivnom. Cl ovome radu data je nova spektrofotometrijska metoda za određivanje ne-enzimski vezanih ugljenih hidrata za proteine seru­ ma, koja se zasniva na reakciji sa fenol-sumpornom kiselinom. Ispitani su i značajni parametri za primenu metode, kao što su uslovi izvođenja hidrolize, građenja hidroksimetil-furfurala i hromofore, kao i mogući ne­ specifični uticaj na reakciju. Metoda se pokazala više osetljivom od spektrofotometrijskog određivanja sa tiobarbituralnom kiselinom. Nađena je bolja korelacija sa sadržajem HbAic određenim jonoizmenjivačkom hromatografijom, povećanje sadržaja ugljenih hidrata kod dijabetičara bilo je značajnije. Pored toga nije ustanovljen nespecifičan uticaj drugoih supstanci na određivanje., The degree of glycemic control may be performed by analysis of serum glycose content, as well as by determination of non-enzymatically protein-bound carbohydrates in blood. Thus, the determination of hemoglobin and serum protein-bound carbohydrates are used in clinical praxis. These determinations were performed commonly by spectrophotometric method based on thiobarbituric acid as reagent. But this method is not sensitive and reproductive enough. In this work a new spectrophotometric method for determination of non-enzymatically serum-protein bound carbohydrates was proposed. In the method phenol-sulfuric acid as reagent was used. All relevant parameters were investigated, as the conditions of hydrolysis and formation hydroxymethyl-furfural, as well as non-specific influences on the reaction. The method was found more sensitive than spectrophoto-metric one with use thiobarbituric acid as reagent. More significant correlation with HbAtc contents, determined by ion-exchange chromatography, was found. The elevation of protein-bound carbohydrates in diabetics was more significant and the non-specific influences on the determination was not found.",
publisher = "Društvo medicinskih biohemičara Jugoslavije",
journal = "Jugoslovenska medicinska biohemija / Yugoslav Medical Biochemistry",
title = "Spektrofotometrijsko određivanje ugljenih hidrata neenzimski vezanih za proteine seruma, A spectrophotometric determination of a non-enzymatically protein-bound carbohydrates in blood",
volume = "11",
number = "3-4",
pages = "120-120",
url = "https://hdl.handle.net/21.15107/rcub_cer_6590"
}

Stojanović, S., Milin, N.,& Đurđić, V.. (1992). Spektrofotometrijsko određivanje ugljenih hidrata neenzimski vezanih za proteine seruma. in Jugoslovenska medicinska biohemija / Yugoslav Medical Biochemistry
Društvo medicinskih biohemičara Jugoslavije., 11(3-4), 120-120.
https://hdl.handle.net/21.15107/rcub_cer_6590

Stojanović S, Milin N, Đurđić V. Spektrofotometrijsko određivanje ugljenih hidrata neenzimski vezanih za proteine seruma. in Jugoslovenska medicinska biohemija / Yugoslav Medical Biochemistry. 1992;11(3-4):120-120.
https://hdl.handle.net/21.15107/rcub_cer_6590 .

Stojanović, Srđan, Milin, Nenad, Đurđić, Vidosava, "Spektrofotometrijsko određivanje ugljenih hidrata neenzimski vezanih za proteine seruma" in Jugoslovenska medicinska biohemija / Yugoslav Medical Biochemistry, 11, no. 3-4 (1992):120-120,
https://hdl.handle.net/21.15107/rcub_cer_6590 .