TY  - JOUR
AU  - Đukanović, Stefana
AU  - Cvetković, Stefana
AU  - Lončarević, Branka
AU  - Lješević, Marija
AU  - Nikolić, Biljana
AU  - Simin, Nataša
AU  - Bekvalac, Kristina
AU  - Kekić, Dušan
AU  - Mitić-Ćulafić, Dragana
PY  - 2020
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3946
AB  - Staphylococcus aureus is one of the leading causative of numerous infections that is resistant to various antibiotics. Since that we are facing with a serious problem of bacterial resistance it is necessary to find a new antibacterial agents in fight with it. Frangula alnus is traditionally used plant but its antibiofilm potential is poorly investigated. The aim of the study was to investigate antibiofilm activity of F. alnus ethyl-acetate extract (FA) toward S. aureus ATCC strains and clinical isolates as well as its influence on respiration in planktonic and biofilm form. The qualitative GCxGC–MS and quantitative LC–MS/MS analysis revealed that FA extract was rich in phenols and flavonoids and emodin, chatechin, and ester 4-ethoxy benzoic acid were the most dominant components. Results obtained through microdillution assay showed that FA possesses strong antibacterial activity. Furthermore, crystal violet staining of biofilm biomass demonstrated that extract had strong effect on biofilm formation of all tested strains while effect on preformed biofilms was less pronounced. The effect on biofilm was confirmed with scanning electron microscopy where the changes in biofilm structure were noticed. The activity of extract on the consumption of O2 and production of CO2 was monitored using the Micro-Oxymax respirometer. Interestingly, respiration of the most strains was decreased in planktonic form as well as in biofilms. Results obtained in this study are a good basis for further research in order to discover the mechanism of action of the FA extract on connection between biofilm and respiration.
PB  - Elsevier
T2  - Industrial Crops & Products
T1  - Antistaphylococcal and biofilm inhibitory activities of Frangula alnus bark ethyl-acetate extract
VL  - 158
SP  - 113013
DO  - 10.1016/j.indcrop.2020.113013
ER  - 

@article{
author = "Đukanović, Stefana and Cvetković, Stefana and Lončarević, Branka and Lješević, Marija and Nikolić, Biljana and Simin, Nataša and Bekvalac, Kristina and Kekić, Dušan and Mitić-Ćulafić, Dragana",
year = "2020",
abstract = "Staphylococcus aureus is one of the leading causative of numerous infections that is resistant to various antibiotics. Since that we are facing with a serious problem of bacterial resistance it is necessary to find a new antibacterial agents in fight with it. Frangula alnus is traditionally used plant but its antibiofilm potential is poorly investigated. The aim of the study was to investigate antibiofilm activity of F. alnus ethyl-acetate extract (FA) toward S. aureus ATCC strains and clinical isolates as well as its influence on respiration in planktonic and biofilm form. The qualitative GCxGC–MS and quantitative LC–MS/MS analysis revealed that FA extract was rich in phenols and flavonoids and emodin, chatechin, and ester 4-ethoxy benzoic acid were the most dominant components. Results obtained through microdillution assay showed that FA possesses strong antibacterial activity. Furthermore, crystal violet staining of biofilm biomass demonstrated that extract had strong effect on biofilm formation of all tested strains while effect on preformed biofilms was less pronounced. The effect on biofilm was confirmed with scanning electron microscopy where the changes in biofilm structure were noticed. The activity of extract on the consumption of O2 and production of CO2 was monitored using the Micro-Oxymax respirometer. Interestingly, respiration of the most strains was decreased in planktonic form as well as in biofilms. Results obtained in this study are a good basis for further research in order to discover the mechanism of action of the FA extract on connection between biofilm and respiration.",
publisher = "Elsevier",
journal = "Industrial Crops & Products",
title = "Antistaphylococcal and biofilm inhibitory activities of Frangula alnus bark ethyl-acetate extract",
volume = "158",
pages = "113013",
doi = "10.1016/j.indcrop.2020.113013"
}

Đukanović, S., Cvetković, S., Lončarević, B., Lješević, M., Nikolić, B., Simin, N., Bekvalac, K., Kekić, D.,& Mitić-Ćulafić, D.. (2020). Antistaphylococcal and biofilm inhibitory activities of Frangula alnus bark ethyl-acetate extract. in Industrial Crops & Products
Elsevier., 158, 113013.
https://doi.org/10.1016/j.indcrop.2020.113013

Đukanović S, Cvetković S, Lončarević B, Lješević M, Nikolić B, Simin N, Bekvalac K, Kekić D, Mitić-Ćulafić D. Antistaphylococcal and biofilm inhibitory activities of Frangula alnus bark ethyl-acetate extract. in Industrial Crops & Products. 2020;158:113013.
doi:10.1016/j.indcrop.2020.113013 .

Đukanović, Stefana, Cvetković, Stefana, Lončarević, Branka, Lješević, Marija, Nikolić, Biljana, Simin, Nataša, Bekvalac, Kristina, Kekić, Dušan, Mitić-Ćulafić, Dragana, "Antistaphylococcal and biofilm inhibitory activities of Frangula alnus bark ethyl-acetate extract" in Industrial Crops & Products, 158 (2020):113013,
https://doi.org/10.1016/j.indcrop.2020.113013 . .