Loos, K.

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a76df92f-a784-4f23-b511-918df1e26c3d
  • Loos, K. (4)
  • Loos, K (1)
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Author's Bibliography

Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium

Gojgić-Cvijović, Gordana; Jakovljević, Dragica; Lončarević, Branka; Todorović, Nina; Pergal, Marija; Ciric, J.; Loos, K.; Beškoski, Vladimir; Vrvić, Miroslav

(Elsevier, 2019)

TY  - JOUR
AU  - Gojgić-Cvijović, Gordana
AU  - Jakovljević, Dragica
AU  - Lončarević, Branka
AU  - Todorović, Nina
AU  - Pergal, Marija
AU  - Ciric, J.
AU  - Loos, K.
AU  - Beškoski, Vladimir
AU  - Vrvić, Miroslav
PY  - 2019
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2503
AB  - The production of levan by Bacillus licheniformis NS032 in a medium based on sugar beet molasses was studied. High polysaccharide yields were produced by using diluted molasses (100-140 g/L of total sugars) with the addition of commercial sucrose up to 200 g/L of total sugars, as well as K2HPO4. A levan yield of 532 g/L was obtained on a medium optimized by response surface methodology, containing 62.6% of sugar originating from molasses, and 4.66 g/L of phosphate, with initial pH value of 7.2. In comparison to the media with 200 and 400 g/L sucrose, in the molasses optimized medium, the observed bacterial growth was faster, while the maximum production of polysaccharide was achieved over a shorter time interval (48 h). The polysaccharide produced in molasses medium had a weight average molecular weight of 5.82 x 10(6) Da, degree of branching 12.68%, viscosity of 0.24 dL/g, and based on methylation analysis and NMR data, it did not significantly differ from levan obtained in the medium with 200 g/L sucrose.
PB  - Elsevier
T2  - International Journal of Biological Macromolecules
T1  - Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium
VL  - 121
SP  - 142
EP  - 151
DO  - 10.1016/j.ijbiomac.2018.10.019
ER  - 
@article{
author = "Gojgić-Cvijović, Gordana and Jakovljević, Dragica and Lončarević, Branka and Todorović, Nina and Pergal, Marija and Ciric, J. and Loos, K. and Beškoski, Vladimir and Vrvić, Miroslav",
year = "2019",
abstract = "The production of levan by Bacillus licheniformis NS032 in a medium based on sugar beet molasses was studied. High polysaccharide yields were produced by using diluted molasses (100-140 g/L of total sugars) with the addition of commercial sucrose up to 200 g/L of total sugars, as well as K2HPO4. A levan yield of 532 g/L was obtained on a medium optimized by response surface methodology, containing 62.6% of sugar originating from molasses, and 4.66 g/L of phosphate, with initial pH value of 7.2. In comparison to the media with 200 and 400 g/L sucrose, in the molasses optimized medium, the observed bacterial growth was faster, while the maximum production of polysaccharide was achieved over a shorter time interval (48 h). The polysaccharide produced in molasses medium had a weight average molecular weight of 5.82 x 10(6) Da, degree of branching 12.68%, viscosity of 0.24 dL/g, and based on methylation analysis and NMR data, it did not significantly differ from levan obtained in the medium with 200 g/L sucrose.",
publisher = "Elsevier",
journal = "International Journal of Biological Macromolecules",
title = "Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium",
volume = "121",
pages = "142-151",
doi = "10.1016/j.ijbiomac.2018.10.019"
}
Gojgić-Cvijović, G., Jakovljević, D., Lončarević, B., Todorović, N., Pergal, M., Ciric, J., Loos, K., Beškoski, V.,& Vrvić, M.. (2019). Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium. in International Journal of Biological Macromolecules
Elsevier., 121, 142-151.
https://doi.org/10.1016/j.ijbiomac.2018.10.019
Gojgić-Cvijović G, Jakovljević D, Lončarević B, Todorović N, Pergal M, Ciric J, Loos K, Beškoski V, Vrvić M. Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium. in International Journal of Biological Macromolecules. 2019;121:142-151.
doi:10.1016/j.ijbiomac.2018.10.019 .
Gojgić-Cvijović, Gordana, Jakovljević, Dragica, Lončarević, Branka, Todorović, Nina, Pergal, Marija, Ciric, J., Loos, K., Beškoski, Vladimir, Vrvić, Miroslav, "Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium" in International Journal of Biological Macromolecules, 121 (2019):142-151,
https://doi.org/10.1016/j.ijbiomac.2018.10.019 . .
57
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Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium

Gojgić-Cvijović, Gordana; Jakovljević, Dragica; Lončarević, Branka; Todorović, Nina; Pergal, Marija; Ciric, J.; Loos, K.; Beškoski, Vladimir; Vrvić, Miroslav

(Elsevier, 2019)

TY  - JOUR
AU  - Gojgić-Cvijović, Gordana
AU  - Jakovljević, Dragica
AU  - Lončarević, Branka
AU  - Todorović, Nina
AU  - Pergal, Marija
AU  - Ciric, J.
AU  - Loos, K.
AU  - Beškoski, Vladimir
AU  - Vrvić, Miroslav
PY  - 2019
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2926
AB  - The production of levan by Bacillus licheniformis NS032 in a medium based on sugar beet molasses was studied. High polysaccharide yields were produced by using diluted molasses (100-140 g/L of total sugars) with the addition of commercial sucrose up to 200 g/L of total sugars, as well as K2HPO4. A levan yield of 532 g/L was obtained on a medium optimized by response surface methodology, containing 62.6% of sugar originating from molasses, and 4.66 g/L of phosphate, with initial pH value of 7.2. In comparison to the media with 200 and 400 g/L sucrose, in the molasses optimized medium, the observed bacterial growth was faster, while the maximum production of polysaccharide was achieved over a shorter time interval (48 h). The polysaccharide produced in molasses medium had a weight average molecular weight of 5.82 x 10(6) Da, degree of branching 12.68%, viscosity of 0.24 dL/g, and based on methylation analysis and NMR data, it did not significantly differ from levan obtained in the medium with 200 g/L sucrose.
PB  - Elsevier
T2  - International Journal of Biological Macromolecules
T1  - Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium
VL  - 121
SP  - 142
EP  - 151
DO  - 10.1016/j.ijbiomac.2018.10.019
ER  - 
@article{
author = "Gojgić-Cvijović, Gordana and Jakovljević, Dragica and Lončarević, Branka and Todorović, Nina and Pergal, Marija and Ciric, J. and Loos, K. and Beškoski, Vladimir and Vrvić, Miroslav",
year = "2019",
abstract = "The production of levan by Bacillus licheniformis NS032 in a medium based on sugar beet molasses was studied. High polysaccharide yields were produced by using diluted molasses (100-140 g/L of total sugars) with the addition of commercial sucrose up to 200 g/L of total sugars, as well as K2HPO4. A levan yield of 532 g/L was obtained on a medium optimized by response surface methodology, containing 62.6% of sugar originating from molasses, and 4.66 g/L of phosphate, with initial pH value of 7.2. In comparison to the media with 200 and 400 g/L sucrose, in the molasses optimized medium, the observed bacterial growth was faster, while the maximum production of polysaccharide was achieved over a shorter time interval (48 h). The polysaccharide produced in molasses medium had a weight average molecular weight of 5.82 x 10(6) Da, degree of branching 12.68%, viscosity of 0.24 dL/g, and based on methylation analysis and NMR data, it did not significantly differ from levan obtained in the medium with 200 g/L sucrose.",
publisher = "Elsevier",
journal = "International Journal of Biological Macromolecules",
title = "Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium",
volume = "121",
pages = "142-151",
doi = "10.1016/j.ijbiomac.2018.10.019"
}
Gojgić-Cvijović, G., Jakovljević, D., Lončarević, B., Todorović, N., Pergal, M., Ciric, J., Loos, K., Beškoski, V.,& Vrvić, M.. (2019). Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium. in International Journal of Biological Macromolecules
Elsevier., 121, 142-151.
https://doi.org/10.1016/j.ijbiomac.2018.10.019
Gojgić-Cvijović G, Jakovljević D, Lončarević B, Todorović N, Pergal M, Ciric J, Loos K, Beškoski V, Vrvić M. Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium. in International Journal of Biological Macromolecules. 2019;121:142-151.
doi:10.1016/j.ijbiomac.2018.10.019 .
Gojgić-Cvijović, Gordana, Jakovljević, Dragica, Lončarević, Branka, Todorović, Nina, Pergal, Marija, Ciric, J., Loos, K., Beškoski, Vladimir, Vrvić, Miroslav, "Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium" in International Journal of Biological Macromolecules, 121 (2019):142-151,
https://doi.org/10.1016/j.ijbiomac.2018.10.019 . .
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High performance alkyd resins synthesized from postconsumer PET bottles

Spasojević, Pavle; Panić, Vesna; Džunuzović, Jasna; Marinković, Aleksandar D.; Woortman, A J J; Loos, K; Popović, Ivanka G.

(The Royal Society of Chemistry, 2015)

TY  - JOUR
AU  - Spasojević, Pavle
AU  - Panić, Vesna
AU  - Džunuzović, Jasna
AU  - Marinković, Aleksandar D.
AU  - Woortman, A J J
AU  - Loos, K
AU  - Popović, Ivanka G.
PY  - 2015
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1632
AB  - The glycolytic recycling of waste PET presents a challenge for the production of secondary value-added products, such as alkyd resins. A way to overcome the unsatisfactory mechanical, drying and chemical resistance properties of alkyds obtained from difunctional glycolyzates was proposed. Waste PET was glycolyzed using multifunctional alcohols: glycerol (G), trimethylolethane (TME), trimethylolpropane (TMP) and pentaerythritol (PE), giving tetra-and hexa-functional glycolyzates and, for comparison, using diethylene glycol (DEG), propylene glycol (PG) and dipropylene glycol (DPG) giving di-functional glycolyzates. The obtained glycolyzates were examined by H-1 and C-13 NMR, FTIR spectroscopy and elemental analysis and further used in the synthesis of alkyd resins. The properties of the prepared alkyd resins (acid, hydroxyl and iodine values, color, average molar masses and molar mass distributions, viscosity, drying time, hardness, flexibility, gloss, adhesion and chemical resistance) were investigated with respect to the functionality and the structure of the used glycolyzates. Alkyd resins derived from multifunctional glycolyzates (TME and TMP) showed considerably enhanced properties compared to those produced from difunctional glycolyzates and also to conventional general purpose resins.
PB  - The Royal Society of Chemistry
T2  - RSC Advances
T1  - High performance alkyd resins synthesized from postconsumer PET bottles
VL  - 5
IS  - 76
SP  - 62273
EP  - 62283
DO  - 10.1039/c5ra11777a
ER  - 
@article{
author = "Spasojević, Pavle and Panić, Vesna and Džunuzović, Jasna and Marinković, Aleksandar D. and Woortman, A J J and Loos, K and Popović, Ivanka G.",
year = "2015",
abstract = "The glycolytic recycling of waste PET presents a challenge for the production of secondary value-added products, such as alkyd resins. A way to overcome the unsatisfactory mechanical, drying and chemical resistance properties of alkyds obtained from difunctional glycolyzates was proposed. Waste PET was glycolyzed using multifunctional alcohols: glycerol (G), trimethylolethane (TME), trimethylolpropane (TMP) and pentaerythritol (PE), giving tetra-and hexa-functional glycolyzates and, for comparison, using diethylene glycol (DEG), propylene glycol (PG) and dipropylene glycol (DPG) giving di-functional glycolyzates. The obtained glycolyzates were examined by H-1 and C-13 NMR, FTIR spectroscopy and elemental analysis and further used in the synthesis of alkyd resins. The properties of the prepared alkyd resins (acid, hydroxyl and iodine values, color, average molar masses and molar mass distributions, viscosity, drying time, hardness, flexibility, gloss, adhesion and chemical resistance) were investigated with respect to the functionality and the structure of the used glycolyzates. Alkyd resins derived from multifunctional glycolyzates (TME and TMP) showed considerably enhanced properties compared to those produced from difunctional glycolyzates and also to conventional general purpose resins.",
publisher = "The Royal Society of Chemistry",
journal = "RSC Advances",
title = "High performance alkyd resins synthesized from postconsumer PET bottles",
volume = "5",
number = "76",
pages = "62273-62283",
doi = "10.1039/c5ra11777a"
}
Spasojević, P., Panić, V., Džunuzović, J., Marinković, A. D., Woortman, A. J. J., Loos, K.,& Popović, I. G.. (2015). High performance alkyd resins synthesized from postconsumer PET bottles. in RSC Advances
The Royal Society of Chemistry., 5(76), 62273-62283.
https://doi.org/10.1039/c5ra11777a
Spasojević P, Panić V, Džunuzović J, Marinković AD, Woortman AJJ, Loos K, Popović IG. High performance alkyd resins synthesized from postconsumer PET bottles. in RSC Advances. 2015;5(76):62273-62283.
doi:10.1039/c5ra11777a .
Spasojević, Pavle, Panić, Vesna, Džunuzović, Jasna, Marinković, Aleksandar D., Woortman, A J J, Loos, K, Popović, Ivanka G., "High performance alkyd resins synthesized from postconsumer PET bottles" in RSC Advances, 5, no. 76 (2015):62273-62283,
https://doi.org/10.1039/c5ra11777a . .
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33
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Surface modification of macroporous poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) resins for improved Candida antarctica lipase B immobilization

Miletić, N.; Rohandi, R.; Vuković, Zorica; Nastasović, Aleksandra; Loos, K.

(Elsevier, 2009)

TY  - JOUR
AU  - Miletić, N.
AU  - Rohandi, R.
AU  - Vuković, Zorica
AU  - Nastasović, Aleksandra
AU  - Loos, K.
PY  - 2009
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/600
AB  - Crosslinked macroporous hydrophilic poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) [abbreviated poly(GMA-co-EGDMA)] with identical chemical structure (60% of glycidyl methacrylate) but with varied average pore sizes (from 30 to 560 nm), specific surface areas (from 13.2 to 106.0 m2/g), specific volumes (from 0.755 to 1.191 cm3/g) and particle sizes (<100 μm-630 μm) were synthesized via suspension polymerization. Modifications of poly(GMA-co-EGDMA) with various diamines (1,2-diaminoethane, 1,4-diaminobutane, 1,6-diaminohexane and 1,8-diaminooctane), 2-fluoroethylamine, glutaraldehyde and cyanuric chloride were carried out. The influence of the interaction between Candida antarctica lipase B (Cal-B) and various carriers during immobilization on the loading and hydrolytic activity (hydrolysis of para-nitrophenyl acetate) of the immobilized Cal-B were studied. Immobilization of Cal-B was performed at different temperatures and pH values. Cal-B immobilized at 30 °C and pH 6.8 was leading to increased activities. Purely physical adsorption between enzyme and copolymer was observed on carriers in which amine or fluorine groups were introduced into the carrier structure by modification with various diamines or 2-fluoroethylamine. As a consequence enzyme loading and activity decreases. In contrary, modification of the poly(GMA-co-EGDMA) with glutaraldehyde and cyanuric chloride results in a covalent connection between enzyme and carrier. The obtained results show a significant increase in Cal-B activity. The influence of the amount of glutaraldehyde and cyanuric chloride used for modification was screened. Increasing the amount of glutaraldehyde or cyanuric chloride used for modification resulted in an increase of the enzyme loading. Consequently, higher amount of glutaraldehyde used led to a higher fraction of the enzyme molecules that are covalently connected on to the carrier. As the amount of glutaraldehyde or cyanuric chloride used for modifications increases, activity of immobilized C. antarctica lipase B primarily increases, showing the highest value for 0.66% and 0.050% w/w, respectively, and subsequently decreases. We could show that Cal-B immobilized on epoxy-containing copolymer modified with glutaraldehyde and cyanuric chloride performs higher activity than free enzyme powder.
PB  - Elsevier
T2  - Reactive and Functional Polymers
T1  - Surface modification of macroporous poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) resins for improved Candida antarctica lipase B immobilization
VL  - 69
IS  - 1
SP  - 68
EP  - 75
DO  - 10.1016/j.reactfunctpolym.2008.11.001
ER  - 
@article{
author = "Miletić, N. and Rohandi, R. and Vuković, Zorica and Nastasović, Aleksandra and Loos, K.",
year = "2009",
abstract = "Crosslinked macroporous hydrophilic poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) [abbreviated poly(GMA-co-EGDMA)] with identical chemical structure (60% of glycidyl methacrylate) but with varied average pore sizes (from 30 to 560 nm), specific surface areas (from 13.2 to 106.0 m2/g), specific volumes (from 0.755 to 1.191 cm3/g) and particle sizes (<100 μm-630 μm) were synthesized via suspension polymerization. Modifications of poly(GMA-co-EGDMA) with various diamines (1,2-diaminoethane, 1,4-diaminobutane, 1,6-diaminohexane and 1,8-diaminooctane), 2-fluoroethylamine, glutaraldehyde and cyanuric chloride were carried out. The influence of the interaction between Candida antarctica lipase B (Cal-B) and various carriers during immobilization on the loading and hydrolytic activity (hydrolysis of para-nitrophenyl acetate) of the immobilized Cal-B were studied. Immobilization of Cal-B was performed at different temperatures and pH values. Cal-B immobilized at 30 °C and pH 6.8 was leading to increased activities. Purely physical adsorption between enzyme and copolymer was observed on carriers in which amine or fluorine groups were introduced into the carrier structure by modification with various diamines or 2-fluoroethylamine. As a consequence enzyme loading and activity decreases. In contrary, modification of the poly(GMA-co-EGDMA) with glutaraldehyde and cyanuric chloride results in a covalent connection between enzyme and carrier. The obtained results show a significant increase in Cal-B activity. The influence of the amount of glutaraldehyde and cyanuric chloride used for modification was screened. Increasing the amount of glutaraldehyde or cyanuric chloride used for modification resulted in an increase of the enzyme loading. Consequently, higher amount of glutaraldehyde used led to a higher fraction of the enzyme molecules that are covalently connected on to the carrier. As the amount of glutaraldehyde or cyanuric chloride used for modifications increases, activity of immobilized C. antarctica lipase B primarily increases, showing the highest value for 0.66% and 0.050% w/w, respectively, and subsequently decreases. We could show that Cal-B immobilized on epoxy-containing copolymer modified with glutaraldehyde and cyanuric chloride performs higher activity than free enzyme powder.",
publisher = "Elsevier",
journal = "Reactive and Functional Polymers",
title = "Surface modification of macroporous poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) resins for improved Candida antarctica lipase B immobilization",
volume = "69",
number = "1",
pages = "68-75",
doi = "10.1016/j.reactfunctpolym.2008.11.001"
}
Miletić, N., Rohandi, R., Vuković, Z., Nastasović, A.,& Loos, K.. (2009). Surface modification of macroporous poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) resins for improved Candida antarctica lipase B immobilization. in Reactive and Functional Polymers
Elsevier., 69(1), 68-75.
https://doi.org/10.1016/j.reactfunctpolym.2008.11.001
Miletić N, Rohandi R, Vuković Z, Nastasović A, Loos K. Surface modification of macroporous poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) resins for improved Candida antarctica lipase B immobilization. in Reactive and Functional Polymers. 2009;69(1):68-75.
doi:10.1016/j.reactfunctpolym.2008.11.001 .
Miletić, N., Rohandi, R., Vuković, Zorica, Nastasović, Aleksandra, Loos, K., "Surface modification of macroporous poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) resins for improved Candida antarctica lipase B immobilization" in Reactive and Functional Polymers, 69, no. 1 (2009):68-75,
https://doi.org/10.1016/j.reactfunctpolym.2008.11.001 . .
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Macroporous poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) resins-Versatile immobilization supports for biocatalysts

Miletić, N.; Vuković, Zorica; Nastasović, Aleksandra; Loos, K.

(Elsevier, 2009)

TY  - JOUR
AU  - Miletić, N.
AU  - Vuković, Zorica
AU  - Nastasović, Aleksandra
AU  - Loos, K.
PY  - 2009
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/597
AB  - Crosslinked macroporous hydrophilic poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate)s [abbreviated poly(GMA-co-EGDMA)] with identical chemical structure (60% of glycidyl methacrylate) but with varied average pore sizes (from 30 to 560 nm), specific surface areas (from 13.2 to 106.0 m2/g), specific volumes (from 0.755 to 1.191 cm3/g) and particle sizes (less than 100-650 μm) were synthesized via suspension polymerization. The influence of the resin properties on the loading of Candida antarctica lipase B (Cal-B) during immobilization and on the hydrolytic (hydrolysis of para-nitrophenyl acetate) and synthetic (ring-opening polymerization of ε-caprolactone) activity of the immobilized Cal-B were studied. Immobilization of Cal-B was performed at different temperatures and pH values. Cal-B immobilized at 30 °C and pH 6.8 was leading to increased activities. By decreasing the resin diameter: (i) the amount of Cal-B adsorbed onto the resin decreases, (ii) the conversion of para-nitrophenyl acetate increases (hydrolytic activity) and (iii) the conversion of ε-caprolactone and the molecular weight of the synthesized poly-ε-caprolactone increases (synthetic activity). Varying the porosity parameters results in different hydrolytic and synthetic activities. Pore sizes of all synthesized resins (from 30 to 560 nm) are big enough to overcome diffusion limitations. Therefore increasing the pore size of the resins resulted in a large increase in the hydrolytic and synthetic activity. Increasing the specific surface area resulted in an increase of activities, as the result of alleviated substrate approach to the immobilized enzyme zones. The obtained results were compared to results from dried Cal-B powder and Novozyme 435. Resin with particle size less than 100 μm and pore size 48 nm had much higher hydrolytic activity than both dried Cal-B powder and Novozyme 435. Nearly similar trends were observed for the synthetic activity. Via the DMSO leaching technique we could show that about 80% of Cal-B was covalently attached to the macroporous resin.
PB  - Elsevier
T2  - Journal of Molecular Catalysis B: Enzymatic
T1  - Macroporous poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) resins-Versatile immobilization supports for biocatalysts
VL  - 56
IS  - 4
SP  - 196
EP  - 201
DO  - 10.1016/j.molcatb.2008.04.012
ER  - 
@article{
author = "Miletić, N. and Vuković, Zorica and Nastasović, Aleksandra and Loos, K.",
year = "2009",
abstract = "Crosslinked macroporous hydrophilic poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate)s [abbreviated poly(GMA-co-EGDMA)] with identical chemical structure (60% of glycidyl methacrylate) but with varied average pore sizes (from 30 to 560 nm), specific surface areas (from 13.2 to 106.0 m2/g), specific volumes (from 0.755 to 1.191 cm3/g) and particle sizes (less than 100-650 μm) were synthesized via suspension polymerization. The influence of the resin properties on the loading of Candida antarctica lipase B (Cal-B) during immobilization and on the hydrolytic (hydrolysis of para-nitrophenyl acetate) and synthetic (ring-opening polymerization of ε-caprolactone) activity of the immobilized Cal-B were studied. Immobilization of Cal-B was performed at different temperatures and pH values. Cal-B immobilized at 30 °C and pH 6.8 was leading to increased activities. By decreasing the resin diameter: (i) the amount of Cal-B adsorbed onto the resin decreases, (ii) the conversion of para-nitrophenyl acetate increases (hydrolytic activity) and (iii) the conversion of ε-caprolactone and the molecular weight of the synthesized poly-ε-caprolactone increases (synthetic activity). Varying the porosity parameters results in different hydrolytic and synthetic activities. Pore sizes of all synthesized resins (from 30 to 560 nm) are big enough to overcome diffusion limitations. Therefore increasing the pore size of the resins resulted in a large increase in the hydrolytic and synthetic activity. Increasing the specific surface area resulted in an increase of activities, as the result of alleviated substrate approach to the immobilized enzyme zones. The obtained results were compared to results from dried Cal-B powder and Novozyme 435. Resin with particle size less than 100 μm and pore size 48 nm had much higher hydrolytic activity than both dried Cal-B powder and Novozyme 435. Nearly similar trends were observed for the synthetic activity. Via the DMSO leaching technique we could show that about 80% of Cal-B was covalently attached to the macroporous resin.",
publisher = "Elsevier",
journal = "Journal of Molecular Catalysis B: Enzymatic",
title = "Macroporous poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) resins-Versatile immobilization supports for biocatalysts",
volume = "56",
number = "4",
pages = "196-201",
doi = "10.1016/j.molcatb.2008.04.012"
}
Miletić, N., Vuković, Z., Nastasović, A.,& Loos, K.. (2009). Macroporous poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) resins-Versatile immobilization supports for biocatalysts. in Journal of Molecular Catalysis B: Enzymatic
Elsevier., 56(4), 196-201.
https://doi.org/10.1016/j.molcatb.2008.04.012
Miletić N, Vuković Z, Nastasović A, Loos K. Macroporous poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) resins-Versatile immobilization supports for biocatalysts. in Journal of Molecular Catalysis B: Enzymatic. 2009;56(4):196-201.
doi:10.1016/j.molcatb.2008.04.012 .
Miletić, N., Vuković, Zorica, Nastasović, Aleksandra, Loos, K., "Macroporous poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) resins-Versatile immobilization supports for biocatalysts" in Journal of Molecular Catalysis B: Enzymatic, 56, no. 4 (2009):196-201,
https://doi.org/10.1016/j.molcatb.2008.04.012 . .
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