TY  - JOUR
AU  - Jurica, Karlo
AU  - Karaconji, Irena Brcic
AU  - Šegan, Sandra
AU  - Milojković-Opsenica, Dušanka
AU  - Kremer, Dario
PY  - 2015
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1792
AB  - The phenolic glycoside arbutin and its metabolite with uroantiseptic activity hydroquinone occur naturally in the leaves of various medicinal plants and spices. In this study, an extraction procedure coupled with gas chromatography-mass spectrometry (GC-MS) was developed to determine arbutin and hydroquinone content in strawberry tree (Arbutus unedo L., Ericaceae) leaves. The method showed good linearity (R-2>0.9987) in the tested concentration range (0.5-200 mu g mL(-1)), as well as good precision (RSD LT 5 %), analytical recovery (96.2-98.0 %), and sensitivity (limit of detection=0.009 and 0.004 mu g mL(-1) for arbutin and hydroquinone, respectively). The results obtained by the validated GC-MS method corresponded well to those obtained by high performance liquid chromatography (HPLC) method. The proposed method was then applied for determining arbutin and hydroquinone content in methanolic leaf extracts. The amount of arbutin in the leaves collected on the island of Kolocep (6.82 mg g(-1) dry weight) was found to be higher (t(paired)=43.57, t(c)=2.92) in comparison to the amount of arbutin in the leaves collected on the island of Mali Losinj (2.75 mg g(-1) dry weight). Hydroquinone was not detected in any of the samples. The analytical features of the proposed GC-MS method demonstrated that arbutin and hydroquinone could be determined alternatively by gas chromatography. Due to its wide concentration range, the method could also be suitable for arbutin and hydroquinone analysis in leaves of other plant families (Rosaceae, Lamiaceae, etc.).
PB  - Inst Medical Research & Occupational Health, Zagreb
T2  - Arhiv Za Higijenu Rada I Toksikologiju-Archives of Industrial Hygiene and Toxicology
T1  - Quantitative analysis of arbutin and hydroquinone in strawberry tree (Arbutus unedo L., Ericaceae) leaves by gas chromatography-mass spectrometry
VL  - 66
IS  - 3
SP  - 197
EP  - 202
DO  - 10.1515/aiht-2015-66-2696
ER  - 

@article{
author = "Jurica, Karlo and Karaconji, Irena Brcic and Šegan, Sandra and Milojković-Opsenica, Dušanka and Kremer, Dario",
year = "2015",
abstract = "The phenolic glycoside arbutin and its metabolite with uroantiseptic activity hydroquinone occur naturally in the leaves of various medicinal plants and spices. In this study, an extraction procedure coupled with gas chromatography-mass spectrometry (GC-MS) was developed to determine arbutin and hydroquinone content in strawberry tree (Arbutus unedo L., Ericaceae) leaves. The method showed good linearity (R-2>0.9987) in the tested concentration range (0.5-200 mu g mL(-1)), as well as good precision (RSD LT 5 %), analytical recovery (96.2-98.0 %), and sensitivity (limit of detection=0.009 and 0.004 mu g mL(-1) for arbutin and hydroquinone, respectively). The results obtained by the validated GC-MS method corresponded well to those obtained by high performance liquid chromatography (HPLC) method. The proposed method was then applied for determining arbutin and hydroquinone content in methanolic leaf extracts. The amount of arbutin in the leaves collected on the island of Kolocep (6.82 mg g(-1) dry weight) was found to be higher (t(paired)=43.57, t(c)=2.92) in comparison to the amount of arbutin in the leaves collected on the island of Mali Losinj (2.75 mg g(-1) dry weight). Hydroquinone was not detected in any of the samples. The analytical features of the proposed GC-MS method demonstrated that arbutin and hydroquinone could be determined alternatively by gas chromatography. Due to its wide concentration range, the method could also be suitable for arbutin and hydroquinone analysis in leaves of other plant families (Rosaceae, Lamiaceae, etc.).",
publisher = "Inst Medical Research & Occupational Health, Zagreb",
journal = "Arhiv Za Higijenu Rada I Toksikologiju-Archives of Industrial Hygiene and Toxicology",
title = "Quantitative analysis of arbutin and hydroquinone in strawberry tree (Arbutus unedo L., Ericaceae) leaves by gas chromatography-mass spectrometry",
volume = "66",
number = "3",
pages = "197-202",
doi = "10.1515/aiht-2015-66-2696"
}

Jurica, K., Karaconji, I. B., Šegan, S., Milojković-Opsenica, D.,& Kremer, D.. (2015). Quantitative analysis of arbutin and hydroquinone in strawberry tree (Arbutus unedo L., Ericaceae) leaves by gas chromatography-mass spectrometry. in Arhiv Za Higijenu Rada I Toksikologiju-Archives of Industrial Hygiene and Toxicology
Inst Medical Research & Occupational Health, Zagreb., 66(3), 197-202.
https://doi.org/10.1515/aiht-2015-66-2696

Jurica K, Karaconji IB, Šegan S, Milojković-Opsenica D, Kremer D. Quantitative analysis of arbutin and hydroquinone in strawberry tree (Arbutus unedo L., Ericaceae) leaves by gas chromatography-mass spectrometry. in Arhiv Za Higijenu Rada I Toksikologiju-Archives of Industrial Hygiene and Toxicology. 2015;66(3):197-202.
doi:10.1515/aiht-2015-66-2696 .

Jurica, Karlo, Karaconji, Irena Brcic, Šegan, Sandra, Milojković-Opsenica, Dušanka, Kremer, Dario, "Quantitative analysis of arbutin and hydroquinone in strawberry tree (Arbutus unedo L., Ericaceae) leaves by gas chromatography-mass spectrometry" in Arhiv Za Higijenu Rada I Toksikologiju-Archives of Industrial Hygiene and Toxicology, 66, no. 3 (2015):197-202,
https://doi.org/10.1515/aiht-2015-66-2696 . .