TY  - JOUR
AU  - Knežević, Aleksandar
AU  - Đokić, Ivana
AU  - Tosti, Tomislav
AU  - Popović, Slađana
AU  - Milojković-Opsenica, Dušanka
AU  - Vukojević, Jelena
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7232
AB  - The aim of the study was the comparative analysis of degradation of wheat straw lignin by white-rot fungi and its  implications on the efficiency of enzymatic hydrolysis of holocellulose. Peroxidases were found to be predominantly  responsible for lignin degradation, even though high laccase activities were detected, especially in the initial stages of  fungal culturing. The studied fungal species showed various ability to degrade lignin in wheat straw, which further  affected the release of reducing sugars during enzymatic saccharification. The highest rate of lignin, hemicelluloses and  cellulose degradation was noticed in the sample pretreated with Irpex lacteus. Among all the tested species, only  Ganoderma resinaceum was found as a suitable lignin degrader, with 2-fold higher hydrolysis yield (51.1 ± 4.7%) than  in the control. A key mechanism that enhances convertibility of carbohydrates is the selective lignin removal from biomass. Operating time, holocellulose loss and unpredictable fungal response to culturing conditions are the main  challenges in fungal pretreatment of lignocellulosic feedstock.
PB  - Institutul de Chimie Macromoleculara Petru Poni
T2  - Cellulose Chemistry and Technology
T1  - White-rot fungal pretreatment of wheat straw: effect on enzymatic hydrolysis of carbohydrate polymers
VL  - 57
IS  - 7-8
SP  - 815
EP  - 828
DO  - 10.35812/CelluloseChemTechnol.2023.57.72
ER  - 

@article{
author = "Knežević, Aleksandar and Đokić, Ivana and Tosti, Tomislav and Popović, Slađana and Milojković-Opsenica, Dušanka and Vukojević, Jelena",
year = "2023",
abstract = "The aim of the study was the comparative analysis of degradation of wheat straw lignin by white-rot fungi and its  implications on the efficiency of enzymatic hydrolysis of holocellulose. Peroxidases were found to be predominantly  responsible for lignin degradation, even though high laccase activities were detected, especially in the initial stages of  fungal culturing. The studied fungal species showed various ability to degrade lignin in wheat straw, which further  affected the release of reducing sugars during enzymatic saccharification. The highest rate of lignin, hemicelluloses and  cellulose degradation was noticed in the sample pretreated with Irpex lacteus. Among all the tested species, only  Ganoderma resinaceum was found as a suitable lignin degrader, with 2-fold higher hydrolysis yield (51.1 ± 4.7%) than  in the control. A key mechanism that enhances convertibility of carbohydrates is the selective lignin removal from biomass. Operating time, holocellulose loss and unpredictable fungal response to culturing conditions are the main  challenges in fungal pretreatment of lignocellulosic feedstock.",
publisher = "Institutul de Chimie Macromoleculara Petru Poni",
journal = "Cellulose Chemistry and Technology",
title = "White-rot fungal pretreatment of wheat straw: effect on enzymatic hydrolysis of carbohydrate polymers",
volume = "57",
number = "7-8",
pages = "815-828",
doi = "10.35812/CelluloseChemTechnol.2023.57.72"
}

Knežević, A., Đokić, I., Tosti, T., Popović, S., Milojković-Opsenica, D.,& Vukojević, J.. (2023). White-rot fungal pretreatment of wheat straw: effect on enzymatic hydrolysis of carbohydrate polymers. in Cellulose Chemistry and Technology
Institutul de Chimie Macromoleculara Petru Poni., 57(7-8), 815-828.
https://doi.org/10.35812/CelluloseChemTechnol.2023.57.72

Knežević A, Đokić I, Tosti T, Popović S, Milojković-Opsenica D, Vukojević J. White-rot fungal pretreatment of wheat straw: effect on enzymatic hydrolysis of carbohydrate polymers. in Cellulose Chemistry and Technology. 2023;57(7-8):815-828.
doi:10.35812/CelluloseChemTechnol.2023.57.72 .

Knežević, Aleksandar, Đokić, Ivana, Tosti, Tomislav, Popović, Slađana, Milojković-Opsenica, Dušanka, Vukojević, Jelena, "White-rot fungal pretreatment of wheat straw: effect on enzymatic hydrolysis of carbohydrate polymers" in Cellulose Chemistry and Technology, 57, no. 7-8 (2023):815-828,
https://doi.org/10.35812/CelluloseChemTechnol.2023.57.72 . .

TY  - JOUR
AU  - Savković, Željko
AU  - Stupar, Miloš
AU  - Unković, Nikola
AU  - Ivanović, Žarko
AU  - Blagojević, Jovana
AU  - Popović, Slađana
AU  - Vukojević, Jelena
AU  - Ljaljević Grbić, Milica
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4051
AB  - An extensive aeromycological survey was performed in the premises of a cultural heritage conservation facility to estimate fungal propagule concentrations in both indoor and outdoor air and seasonal dynamics with regard to temperature and air humidity. The highest average propagule concentration in indoor air was documented in spring, and for outdoor air, in summer. Increased propagule loads were documented in most rooms during winter, fall and spring, and in all examined rooms in summer. Very high propagule concentrations (>25,000 CFU m−3) were reported in ground-floor rooms. The total mycobiota was comprised of 74 fungal species, with a prevalence of Aspergilli and Penicillia. Cladosporium and Penicillium isolates were found to be the most abundant in all seasons in both indoor and outdoor air samples. Among the identified fungi, potential human pathogens, allergens and mycotoxin producers were present. Numerous documented species play an important role in the deterioration of cultural heritage artifacts and are known producers of extracellular enzymes, acids and pigments. Application of a selective medium (M40Y) allowed isolation of various xerophiles and xerotolerant species. Aeromycological analyses are mandatory for determining the appropriate conditions for the protection of the health of conservators, as well as the objects of cultural heritage.
PB  - Elsevier
T2  - International Biodeterioration & Biodegradation
T1  - Diversity and seasonal dynamics of culturable airborne fungi in a cultural heritage conservation facility
VL  - 157
SP  - 105163
DO  - 10.1016/j.ibiod.2020.105163
ER  - 

@article{
author = "Savković, Željko and Stupar, Miloš and Unković, Nikola and Ivanović, Žarko and Blagojević, Jovana and Popović, Slađana and Vukojević, Jelena and Ljaljević Grbić, Milica",
year = "2021",
abstract = "An extensive aeromycological survey was performed in the premises of a cultural heritage conservation facility to estimate fungal propagule concentrations in both indoor and outdoor air and seasonal dynamics with regard to temperature and air humidity. The highest average propagule concentration in indoor air was documented in spring, and for outdoor air, in summer. Increased propagule loads were documented in most rooms during winter, fall and spring, and in all examined rooms in summer. Very high propagule concentrations (>25,000 CFU m−3) were reported in ground-floor rooms. The total mycobiota was comprised of 74 fungal species, with a prevalence of Aspergilli and Penicillia. Cladosporium and Penicillium isolates were found to be the most abundant in all seasons in both indoor and outdoor air samples. Among the identified fungi, potential human pathogens, allergens and mycotoxin producers were present. Numerous documented species play an important role in the deterioration of cultural heritage artifacts and are known producers of extracellular enzymes, acids and pigments. Application of a selective medium (M40Y) allowed isolation of various xerophiles and xerotolerant species. Aeromycological analyses are mandatory for determining the appropriate conditions for the protection of the health of conservators, as well as the objects of cultural heritage.",
publisher = "Elsevier",
journal = "International Biodeterioration & Biodegradation",
title = "Diversity and seasonal dynamics of culturable airborne fungi in a cultural heritage conservation facility",
volume = "157",
pages = "105163",
doi = "10.1016/j.ibiod.2020.105163"
}

Savković, Ž., Stupar, M., Unković, N., Ivanović, Ž., Blagojević, J., Popović, S., Vukojević, J.,& Ljaljević Grbić, M.. (2021). Diversity and seasonal dynamics of culturable airborne fungi in a cultural heritage conservation facility. in International Biodeterioration & Biodegradation
Elsevier., 157, 105163.
https://doi.org/10.1016/j.ibiod.2020.105163

Savković Ž, Stupar M, Unković N, Ivanović Ž, Blagojević J, Popović S, Vukojević J, Ljaljević Grbić M. Diversity and seasonal dynamics of culturable airborne fungi in a cultural heritage conservation facility. in International Biodeterioration & Biodegradation. 2021;157:105163.
doi:10.1016/j.ibiod.2020.105163 .

Savković, Željko, Stupar, Miloš, Unković, Nikola, Ivanović, Žarko, Blagojević, Jovana, Popović, Slađana, Vukojević, Jelena, Ljaljević Grbić, Milica, "Diversity and seasonal dynamics of culturable airborne fungi in a cultural heritage conservation facility" in International Biodeterioration & Biodegradation, 157 (2021):105163,
https://doi.org/10.1016/j.ibiod.2020.105163 . .

TY  - JOUR
AU  - Unković, Nikola
AU  - Popović, Slađana
AU  - Stupar, Miloš
AU  - Samolov, Elena
AU  - Subakov Simić, Gordana
AU  - Ljaljević Grbić, Milica
AU  - Vukojević, Jelena
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4748
AB  - Diversity of subaerial biofilm forming cyanobacteria, algae and fungi was investigated on 10 different substrata from greenhouse of Botanical Garden “Jevremovac”. Out of 37 documented taxa, 16 cyanobacterial and 10 algal taxa were identified. Remaining 11 taxa belong to the Kingdom of Fungi. The highest diversity of biofilm forming microorganisms, a total of 24 taxa, was detected on the corroded metal surface, while significantly lower number of taxa was recorded on other examined substrata. Cyanobacterium Porphyrosiphon sp., diatom Achnanthes sp. and green algae Chlorella sp. and Chlorococcum minutum were the most frequently encountered photosynthetic components of biofilms. In all analyzed samples, Trichoderma sp., followed by Cladosporium sp. and Rhizopus stolonifer, were the most frequently identified fungi.
AB  - Испитиван је диверзитет цијанобактерија, алги и гљива у биофилму с 10 различитих супстрата из стакленика Ботаничке баште „Јевремовац“. Од 37 документованих таксона, идентификовано је 16 цијанобактерија и 10 алги. Преосталих 11 таксона припадају „Петом царству“. Највећа разноврсност микроорганизама, укупно 24 таксона, забележена је у биофилму на кородираној металној површини, док је значајно нижи број таксона регистрован на осталим испитиваним супстратима. Цијанобактерија Porphyrosiphon sp., дијатома Achnanthes sp. и зелене алге Chlorella sp. и Chlorococcum minutum су најчешће фотосинтетичке компоненте биофилма. У свим испитиваним узорцима Trichoderma sp., заједно са Cladosporium sp. и Rhizopus stolonifer су најчешће идентификоване гљиве.
PB  - Novi Sad : Matica srpska
T2  - Zbornik Matice Srpske za Prirodne Nauke
T1  - Biofilm forming microorganisms on various substrata from greenhouse of Botanical Garden “Jevremovac”
T1  - Mikroorganizmi u sastavu biofilma s različitih supstrata staklenika Botaničke bašte “Jevremovac”
VL  - 2017
IS  - 32
SP  - 57
EP  - 67
DO  - 10.2298/ZMSPN1732057U
ER  - 

@article{
author = "Unković, Nikola and Popović, Slađana and Stupar, Miloš and Samolov, Elena and Subakov Simić, Gordana and Ljaljević Grbić, Milica and Vukojević, Jelena",
year = "2017",
abstract = "Diversity of subaerial biofilm forming cyanobacteria, algae and fungi was investigated on 10 different substrata from greenhouse of Botanical Garden “Jevremovac”. Out of 37 documented taxa, 16 cyanobacterial and 10 algal taxa were identified. Remaining 11 taxa belong to the Kingdom of Fungi. The highest diversity of biofilm forming microorganisms, a total of 24 taxa, was detected on the corroded metal surface, while significantly lower number of taxa was recorded on other examined substrata. Cyanobacterium Porphyrosiphon sp., diatom Achnanthes sp. and green algae Chlorella sp. and Chlorococcum minutum were the most frequently encountered photosynthetic components of biofilms. In all analyzed samples, Trichoderma sp., followed by Cladosporium sp. and Rhizopus stolonifer, were the most frequently identified fungi., Испитиван је диверзитет цијанобактерија, алги и гљива у биофилму с 10 различитих супстрата из стакленика Ботаничке баште „Јевремовац“. Од 37 документованих таксона, идентификовано је 16 цијанобактерија и 10 алги. Преосталих 11 таксона припадају „Петом царству“. Највећа разноврсност микроорганизама, укупно 24 таксона, забележена је у биофилму на кородираној металној површини, док је значајно нижи број таксона регистрован на осталим испитиваним супстратима. Цијанобактерија Porphyrosiphon sp., дијатома Achnanthes sp. и зелене алге Chlorella sp. и Chlorococcum minutum су најчешће фотосинтетичке компоненте биофилма. У свим испитиваним узорцима Trichoderma sp., заједно са Cladosporium sp. и Rhizopus stolonifer су најчешће идентификоване гљиве.",
publisher = "Novi Sad : Matica srpska",
journal = "Zbornik Matice Srpske za Prirodne Nauke",
title = "Biofilm forming microorganisms on various substrata from greenhouse of Botanical Garden “Jevremovac”, Mikroorganizmi u sastavu biofilma s različitih supstrata staklenika Botaničke bašte “Jevremovac”",
volume = "2017",
number = "32",
pages = "57-67",
doi = "10.2298/ZMSPN1732057U"
}

Unković, N., Popović, S., Stupar, M., Samolov, E., Subakov Simić, G., Ljaljević Grbić, M.,& Vukojević, J.. (2017). Biofilm forming microorganisms on various substrata from greenhouse of Botanical Garden “Jevremovac”. in Zbornik Matice Srpske za Prirodne Nauke
Novi Sad : Matica srpska., 2017(32), 57-67.
https://doi.org/10.2298/ZMSPN1732057U

Unković N, Popović S, Stupar M, Samolov E, Subakov Simić G, Ljaljević Grbić M, Vukojević J. Biofilm forming microorganisms on various substrata from greenhouse of Botanical Garden “Jevremovac”. in Zbornik Matice Srpske za Prirodne Nauke. 2017;2017(32):57-67.
doi:10.2298/ZMSPN1732057U .

Unković, Nikola, Popović, Slađana, Stupar, Miloš, Samolov, Elena, Subakov Simić, Gordana, Ljaljević Grbić, Milica, Vukojević, Jelena, "Biofilm forming microorganisms on various substrata from greenhouse of Botanical Garden “Jevremovac”" in Zbornik Matice Srpske za Prirodne Nauke, 2017, no. 32 (2017):57-67,
https://doi.org/10.2298/ZMSPN1732057U . .