TY  - JOUR
AU  - Khalife, Majd
AU  - Stanković, Dalibor
AU  - Stanković, Vesna
AU  - Danicka, Julia
AU  - Rizzotto, Francesco
AU  - Costache, Vlad
AU  - Schwok, Anny Slama
AU  - Gaudu, Philippe
AU  - Vidić, Jasmina
PY  - 2024
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6896
AB  - Vitamin K refers to a group of vitamins that play an important role in blood coagulation and regulation of bone and vascular metabolism. However, vitamin K3 may give severe side effects in animal and humans when improperly added to food and feed due to its toxicity. Here, an electrochemical biosensor, based on the YaiB NADPH-dependent quinone reductase from Lactococcus lactis (YaiB), was developed to achieve rapid and redox probe-free detection of vitamin K3. First, the ability of the carbon electrode to distinguish between 1,4-benzoquinone and hydroquinone was demonstrated. Then, we engineered YaiB to work as a bioreceptor immobilized at the electrode and its sensitivity and specificity to reduce vitamin K3 were demonstrated. Finally, to demonstrate the practical potential of the biosensor, we tested it directly in spiked milk samples, achieving 15-minute quantification of the vitamin K3. The limit of detection was 0.87 µM and 4.1 µM in buffer and milk, respectively.
PB  - Elsevier
T2  - Food Chemistry
T1  - Electrochemical biosensor based on NAD(P)H-dependent quinone reductase for rapid and efficient detection of vitamin K3
VL  - 433
SP  - 137316
DO  - 10.1016/j.foodchem.2023.137316
ER  - 

@article{
author = "Khalife, Majd and Stanković, Dalibor and Stanković, Vesna and Danicka, Julia and Rizzotto, Francesco and Costache, Vlad and Schwok, Anny Slama and Gaudu, Philippe and Vidić, Jasmina",
year = "2024",
abstract = "Vitamin K refers to a group of vitamins that play an important role in blood coagulation and regulation of bone and vascular metabolism. However, vitamin K3 may give severe side effects in animal and humans when improperly added to food and feed due to its toxicity. Here, an electrochemical biosensor, based on the YaiB NADPH-dependent quinone reductase from Lactococcus lactis (YaiB), was developed to achieve rapid and redox probe-free detection of vitamin K3. First, the ability of the carbon electrode to distinguish between 1,4-benzoquinone and hydroquinone was demonstrated. Then, we engineered YaiB to work as a bioreceptor immobilized at the electrode and its sensitivity and specificity to reduce vitamin K3 were demonstrated. Finally, to demonstrate the practical potential of the biosensor, we tested it directly in spiked milk samples, achieving 15-minute quantification of the vitamin K3. The limit of detection was 0.87 µM and 4.1 µM in buffer and milk, respectively.",
publisher = "Elsevier",
journal = "Food Chemistry",
title = "Electrochemical biosensor based on NAD(P)H-dependent quinone reductase for rapid and efficient detection of vitamin K3",
volume = "433",
pages = "137316",
doi = "10.1016/j.foodchem.2023.137316"
}

Khalife, M., Stanković, D., Stanković, V., Danicka, J., Rizzotto, F., Costache, V., Schwok, A. S., Gaudu, P.,& Vidić, J.. (2024). Electrochemical biosensor based on NAD(P)H-dependent quinone reductase for rapid and efficient detection of vitamin K3. in Food Chemistry
Elsevier., 433, 137316.
https://doi.org/10.1016/j.foodchem.2023.137316

Khalife M, Stanković D, Stanković V, Danicka J, Rizzotto F, Costache V, Schwok AS, Gaudu P, Vidić J. Electrochemical biosensor based on NAD(P)H-dependent quinone reductase for rapid and efficient detection of vitamin K3. in Food Chemistry. 2024;433:137316.
doi:10.1016/j.foodchem.2023.137316 .

Khalife, Majd, Stanković, Dalibor, Stanković, Vesna, Danicka, Julia, Rizzotto, Francesco, Costache, Vlad, Schwok, Anny Slama, Gaudu, Philippe, Vidić, Jasmina, "Electrochemical biosensor based on NAD(P)H-dependent quinone reductase for rapid and efficient detection of vitamin K3" in Food Chemistry, 433 (2024):137316,
https://doi.org/10.1016/j.foodchem.2023.137316 . .

TY  - JOUR
AU  - Knežević, Sara
AU  - Terzić-Jovanović, Nataša
AU  - Vlahović, Filip
AU  - Ajdačić, Vladimir
AU  - Costache, Vlad
AU  - Vidić, Jasmina
AU  - Opsenica, Igor
AU  - Stanković, Dalibor
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7175
AB  - Covalent organic frameworks (COFs) are emerging as promising sensing materials due to their controllable structure and function properties, as well as excellent physicochemical characteristics. Here, specific interactions between a triazine-based COF and a mass-used herbicide – glyphosate (GLY) have been utilized to design a disposable sensing platform for GLY detection. This herbicide has been extensively used for decades, however, its harmful environmental impact and toxicity to humans have been recently proven, conditioning the necessity for the strict control and monitoring of its use and its presence in soil, water, and food. Glyphosate is an organophosphorus compound, and its detection in complex matrices usually requires laborious pretreatment. Here, we developed a direct, miniaturized, robust, and green approach for disposable electrochemical sensing of glyphosate, utilizing COF's ability to selectively capture and concentrate negatively charged glyphosate molecules inside its nanopores. This process generates the concentration gradient of GLY, accelerating its diffusion towards the electrode surface. Simultaneously, specific COF-glyphosate binding catalyses the oxidative cleavage of the C–P bond and, together with pore nanoconfinement, enables sensitive glyphosate detection. Detailed sensing principles and selectiveness were scrutinized using DFT-based modelling. The proposed electrochemical method has a linear working range from 0.1 μM to 10 μM, a low limit of detection of 96 nM, and a limit of quantification of 320 nM. The elaborated sensing approach is viable for use in real sample matrices and tested for GLY determination in soil and water samples, without pretreatment, preparation, or purification. The results showed the practical usefulness of the sensor in the real sample analysis and suggested its suitability for possible out-of-laboratory sensing.
PB  - Elsevier
T2  - Chemosphere
T1  - Direct glyphosate soil monitoring at the triazine-based covalent organic framework with the theoretical study of sensing principle
VL  - 341
SP  - 139930
DO  - 10.1016/j.chemosphere.2023.139930
ER  - 

@article{
author = "Knežević, Sara and Terzić-Jovanović, Nataša and Vlahović, Filip and Ajdačić, Vladimir and Costache, Vlad and Vidić, Jasmina and Opsenica, Igor and Stanković, Dalibor",
year = "2023",
abstract = "Covalent organic frameworks (COFs) are emerging as promising sensing materials due to their controllable structure and function properties, as well as excellent physicochemical characteristics. Here, specific interactions between a triazine-based COF and a mass-used herbicide – glyphosate (GLY) have been utilized to design a disposable sensing platform for GLY detection. This herbicide has been extensively used for decades, however, its harmful environmental impact and toxicity to humans have been recently proven, conditioning the necessity for the strict control and monitoring of its use and its presence in soil, water, and food. Glyphosate is an organophosphorus compound, and its detection in complex matrices usually requires laborious pretreatment. Here, we developed a direct, miniaturized, robust, and green approach for disposable electrochemical sensing of glyphosate, utilizing COF's ability to selectively capture and concentrate negatively charged glyphosate molecules inside its nanopores. This process generates the concentration gradient of GLY, accelerating its diffusion towards the electrode surface. Simultaneously, specific COF-glyphosate binding catalyses the oxidative cleavage of the C–P bond and, together with pore nanoconfinement, enables sensitive glyphosate detection. Detailed sensing principles and selectiveness were scrutinized using DFT-based modelling. The proposed electrochemical method has a linear working range from 0.1 μM to 10 μM, a low limit of detection of 96 nM, and a limit of quantification of 320 nM. The elaborated sensing approach is viable for use in real sample matrices and tested for GLY determination in soil and water samples, without pretreatment, preparation, or purification. The results showed the practical usefulness of the sensor in the real sample analysis and suggested its suitability for possible out-of-laboratory sensing.",
publisher = "Elsevier",
journal = "Chemosphere",
title = "Direct glyphosate soil monitoring at the triazine-based covalent organic framework with the theoretical study of sensing principle",
volume = "341",
pages = "139930",
doi = "10.1016/j.chemosphere.2023.139930"
}

Knežević, S., Terzić-Jovanović, N., Vlahović, F., Ajdačić, V., Costache, V., Vidić, J., Opsenica, I.,& Stanković, D.. (2023). Direct glyphosate soil monitoring at the triazine-based covalent organic framework with the theoretical study of sensing principle. in Chemosphere
Elsevier., 341, 139930.
https://doi.org/10.1016/j.chemosphere.2023.139930

Knežević S, Terzić-Jovanović N, Vlahović F, Ajdačić V, Costache V, Vidić J, Opsenica I, Stanković D. Direct glyphosate soil monitoring at the triazine-based covalent organic framework with the theoretical study of sensing principle. in Chemosphere. 2023;341:139930.
doi:10.1016/j.chemosphere.2023.139930 .

Knežević, Sara, Terzić-Jovanović, Nataša, Vlahović, Filip, Ajdačić, Vladimir, Costache, Vlad, Vidić, Jasmina, Opsenica, Igor, Stanković, Dalibor, "Direct glyphosate soil monitoring at the triazine-based covalent organic framework with the theoretical study of sensing principle" in Chemosphere, 341 (2023):139930,
https://doi.org/10.1016/j.chemosphere.2023.139930 . .