Kalcher, K

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  • Kalcher, K (1)
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Enzymatic polyphenol index biosensor based on Graphene nanoplatelets decorated with MnO2 Nanoparticles. Preparation, characterization and Analytical application

Đurđić, Slađana; Vukojević, Vesna; Vlahović, Filip; Ognjanović, Miloš; Kalcher, K; Mutić, Jelena; Stanković, Dalibor

(University of Pardubice, 2019, 2019)

TY  - CONF
AU  - Đurđić, Slađana
AU  - Vukojević, Vesna
AU  - Vlahović, Filip
AU  - Ognjanović, Miloš
AU  - Kalcher, K
AU  - Mutić, Jelena
AU  - Stanković, Dalibor
PY  - 2019
UR  - http://yisac2019.upce.cz/
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3226
AB  - A nanocomposite formed from graphene nanoplatelets (GNP) and manganese oxide (MnO2) nanoparticles (GNP/MnO2) was proposed as a novel and suitable support for enzyme immobilisation. The performances of screen-printed carbon electrodes (SPCEs) was highly improved after modification with GNP/MnO2 (SPCE/GNP/MnO2). The polyphenol index biosensor was prepared by surface modification of SPCE/GNP/MnO2 with drop coating of the laccase (from Trametes Versicolor) and Nafion®. All electrochemical measurements were carried out in acetate buffer, pH=4.60. The developed laccase biosensor shows fast and reliable amperometric response toward caffeic acid, as model compound, at operating potential of +0.40 V (vs. Ag/AgCl), with a linear range from 5 μmol L-1 to 2.75 mmol L-1 (r2 = 0.9997), with detection limit of 2.38 μmol L-1. Moreover, effects of possible interfering compounds were investigated. The developed procedure was successfully applied for the determination of total polyphenol content in red and white wine samples. In order to validate the proposed method, the polyphenol content in wine samples, under optimized parameters, was determined using a glassy carbon electrode. Recovery tests (95.7-97.5%) shows satisfactory accuracy and precision of the developed method, concluding that proposed construction of biosensor can offer fast, stable and reproducible determination of the polyphenol index.
PB  - University of Pardubice, 2019
C3  - Book of Abstracts - YISAC 2019 (26th Young Investigators’ Seminar on Analytical Chemistry)
T1  - Enzymatic polyphenol index biosensor based on Graphene nanoplatelets decorated with MnO2 Nanoparticles. Preparation, characterization and Analytical application
SP  - 38
UR  - https://hdl.handle.net/21.15107/rcub_cer_3226
ER  - 
@conference{
author = "Đurđić, Slađana and Vukojević, Vesna and Vlahović, Filip and Ognjanović, Miloš and Kalcher, K and Mutić, Jelena and Stanković, Dalibor",
year = "2019",
abstract = "A nanocomposite formed from graphene nanoplatelets (GNP) and manganese oxide (MnO2) nanoparticles (GNP/MnO2) was proposed as a novel and suitable support for enzyme immobilisation. The performances of screen-printed carbon electrodes (SPCEs) was highly improved after modification with GNP/MnO2 (SPCE/GNP/MnO2). The polyphenol index biosensor was prepared by surface modification of SPCE/GNP/MnO2 with drop coating of the laccase (from Trametes Versicolor) and Nafion®. All electrochemical measurements were carried out in acetate buffer, pH=4.60. The developed laccase biosensor shows fast and reliable amperometric response toward caffeic acid, as model compound, at operating potential of +0.40 V (vs. Ag/AgCl), with a linear range from 5 μmol L-1 to 2.75 mmol L-1 (r2 = 0.9997), with detection limit of 2.38 μmol L-1. Moreover, effects of possible interfering compounds were investigated. The developed procedure was successfully applied for the determination of total polyphenol content in red and white wine samples. In order to validate the proposed method, the polyphenol content in wine samples, under optimized parameters, was determined using a glassy carbon electrode. Recovery tests (95.7-97.5%) shows satisfactory accuracy and precision of the developed method, concluding that proposed construction of biosensor can offer fast, stable and reproducible determination of the polyphenol index.",
publisher = "University of Pardubice, 2019",
journal = "Book of Abstracts - YISAC 2019 (26th Young Investigators’ Seminar on Analytical Chemistry)",
title = "Enzymatic polyphenol index biosensor based on Graphene nanoplatelets decorated with MnO2 Nanoparticles. Preparation, characterization and Analytical application",
pages = "38",
url = "https://hdl.handle.net/21.15107/rcub_cer_3226"
}
Đurđić, S., Vukojević, V., Vlahović, F., Ognjanović, M., Kalcher, K., Mutić, J.,& Stanković, D.. (2019). Enzymatic polyphenol index biosensor based on Graphene nanoplatelets decorated with MnO2 Nanoparticles. Preparation, characterization and Analytical application. in Book of Abstracts - YISAC 2019 (26th Young Investigators’ Seminar on Analytical Chemistry)
University of Pardubice, 2019., 38.
https://hdl.handle.net/21.15107/rcub_cer_3226
Đurđić S, Vukojević V, Vlahović F, Ognjanović M, Kalcher K, Mutić J, Stanković D. Enzymatic polyphenol index biosensor based on Graphene nanoplatelets decorated with MnO2 Nanoparticles. Preparation, characterization and Analytical application. in Book of Abstracts - YISAC 2019 (26th Young Investigators’ Seminar on Analytical Chemistry). 2019;:38.
https://hdl.handle.net/21.15107/rcub_cer_3226 .
Đurđić, Slađana, Vukojević, Vesna, Vlahović, Filip, Ognjanović, Miloš, Kalcher, K, Mutić, Jelena, Stanković, Dalibor, "Enzymatic polyphenol index biosensor based on Graphene nanoplatelets decorated with MnO2 Nanoparticles. Preparation, characterization and Analytical application" in Book of Abstracts - YISAC 2019 (26th Young Investigators’ Seminar on Analytical Chemistry) (2019):38,
https://hdl.handle.net/21.15107/rcub_cer_3226 .