TY  - JOUR
AU  - Miladinovic, Dragoljub L.
AU  - Ilic, Budimir S.
AU  - Najman, Stevo J.
AU  - Cvetković, Olga
AU  - Šajnović, Aleksandra
AU  - Miladinović, Marija D.
AU  - Nikolic, Nikola D.
PY  - 2013
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1320
AB  - The aim of this study was to research the seasonal changes of antioxidant enzyme activity and total antioxidant capacity in leaves of Astragalus onobrychis L. subsp. chlorocarpus (Griseb.) S. Kozuharov et D.K. Pavlova. Leaves of A. onobrychis were collected during the different stages of growth and analyzed for antioxidant enzyme activity: superoxide dismutase, catalase, guaiacol peroxidase, glutathione peroxidase. Quantities of malonyldialdehyde, superoxide radicals, and hydroxyl radicals were measured as well as the content of soluble proteins. Furthermore, total antioxidant capacity was determined by the inhibition of chemiluminescence activity of blood phagocytes by leaf extracts. Stages of vegetation significantly affected the accumulation of superoxide radicals, but there were no significant differences in hydroxyl radical quantity and lipid peroxidation levels during vegetation. Soluble proteins vary greatly between different stages of growth. Seasonal changes were found to have an effect on enzymatic activities. During the spring season, guaiacol peroxidase showed the highest levels. Catalase and glutathione peroxidase increased their activities in summer, while, during the autumn season, superoxide dismutase showed maximum activity. On the basis of chemiluminescence assay, it can be concluded that leaf extract of A. onobrychis possesses a significant antioxidant capacity thus protecting plants during environmental stress.
PB  - Versita, Warsaw
T2  - Central European Journal of Chemistry
T1  - Antioxidative responses to seasonal changes and chemiluminescence assay of Astragalus onobrychis leaves extract
VL  - 11
IS  - 2
SP  - 123
EP  - 132
DO  - 10.2478/s11532-012-0163-6
ER  - 

@article{
author = "Miladinovic, Dragoljub L. and Ilic, Budimir S. and Najman, Stevo J. and Cvetković, Olga and Šajnović, Aleksandra and Miladinović, Marija D. and Nikolic, Nikola D.",
year = "2013",
abstract = "The aim of this study was to research the seasonal changes of antioxidant enzyme activity and total antioxidant capacity in leaves of Astragalus onobrychis L. subsp. chlorocarpus (Griseb.) S. Kozuharov et D.K. Pavlova. Leaves of A. onobrychis were collected during the different stages of growth and analyzed for antioxidant enzyme activity: superoxide dismutase, catalase, guaiacol peroxidase, glutathione peroxidase. Quantities of malonyldialdehyde, superoxide radicals, and hydroxyl radicals were measured as well as the content of soluble proteins. Furthermore, total antioxidant capacity was determined by the inhibition of chemiluminescence activity of blood phagocytes by leaf extracts. Stages of vegetation significantly affected the accumulation of superoxide radicals, but there were no significant differences in hydroxyl radical quantity and lipid peroxidation levels during vegetation. Soluble proteins vary greatly between different stages of growth. Seasonal changes were found to have an effect on enzymatic activities. During the spring season, guaiacol peroxidase showed the highest levels. Catalase and glutathione peroxidase increased their activities in summer, while, during the autumn season, superoxide dismutase showed maximum activity. On the basis of chemiluminescence assay, it can be concluded that leaf extract of A. onobrychis possesses a significant antioxidant capacity thus protecting plants during environmental stress.",
publisher = "Versita, Warsaw",
journal = "Central European Journal of Chemistry",
title = "Antioxidative responses to seasonal changes and chemiluminescence assay of Astragalus onobrychis leaves extract",
volume = "11",
number = "2",
pages = "123-132",
doi = "10.2478/s11532-012-0163-6"
}

Miladinovic, D. L., Ilic, B. S., Najman, S. J., Cvetković, O., Šajnović, A., Miladinović, M. D.,& Nikolic, N. D.. (2013). Antioxidative responses to seasonal changes and chemiluminescence assay of Astragalus onobrychis leaves extract. in Central European Journal of Chemistry
Versita, Warsaw., 11(2), 123-132.
https://doi.org/10.2478/s11532-012-0163-6

Miladinovic DL, Ilic BS, Najman SJ, Cvetković O, Šajnović A, Miladinović MD, Nikolic ND. Antioxidative responses to seasonal changes and chemiluminescence assay of Astragalus onobrychis leaves extract. in Central European Journal of Chemistry. 2013;11(2):123-132.
doi:10.2478/s11532-012-0163-6 .

Miladinovic, Dragoljub L., Ilic, Budimir S., Najman, Stevo J., Cvetković, Olga, Šajnović, Aleksandra, Miladinović, Marija D., Nikolic, Nikola D., "Antioxidative responses to seasonal changes and chemiluminescence assay of Astragalus onobrychis leaves extract" in Central European Journal of Chemistry, 11, no. 2 (2013):123-132,
https://doi.org/10.2478/s11532-012-0163-6 . .

TY  - JOUR
AU  - Miladinovic, Dragoljub L.
AU  - Ilic, Budimir S.
AU  - Mihajilov-Krstev, Tatjana M.
AU  - Nikolic, Nikola D.
AU  - Miladinovic, Ljiljana C.
AU  - Cvetković, Olga
PY  - 2012
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/954
AB  - The antibacterial effects of Thymus vulgaris (Lamiaceae), Lavandula angustifolia (Lamiaceae), and Calamintha nepeta (Lamiaceae) Savi subsp. nepeta var. subisodonda (Borb.) Hayek essential oils on five different bacteria were estimated. Laboratory control strain and clinical isolates from different pathogenic media were researched by broth microdilution method, with an emphasis on a chemical composition-antibacterial activity relationship. The main constituents of thyme oil were thymol (59.95%) and p-cymene (18.34%). Linalool acetate (38.23%) and beta-linalool (35.01%) were main compounds in lavender oil. C. nepeta essential oil was characterized by a high percentage of piperitone oxide (59.07%) and limonene (9.05%). Essential oils have been found to have antimicrobial activity against all tested microorganisms. Classification and comparison of essential oils on the basis of their chemical composition and antibacterial activity were made by utilization of appropriate chemometric methods. The chemical principal component analysis (PCA) and hierachical cluster analysis (HCA) separated essential oils into two groups and two sub-groups. Thyme essential oil forms separate chemical HCA group and exhibits highest antibacterial activity, similar to tetracycline. Essential oils of lavender and C. nepeta in the same chemical HCA group were classified in different groups, within antibacterial PCA and HCA analyses. Lavender oil exhibits higher antibacterial ability in comparison with C. nepeta essential oil, probably based on the concept of synergistic activity of essential oil components.
PB  - Springer Heidelberg, Heidelberg
T2  - Analytical and Bioanalytical Chemistry
T1  - Investigation of the chemical composition-antibacterial activity relationship of essential oils by chemometric methods
VL  - 403
IS  - 4
SP  - 1007
EP  - 1018
DO  - 10.1007/s00216-012-5866-1
ER  - 

@article{
author = "Miladinovic, Dragoljub L. and Ilic, Budimir S. and Mihajilov-Krstev, Tatjana M. and Nikolic, Nikola D. and Miladinovic, Ljiljana C. and Cvetković, Olga",
year = "2012",
abstract = "The antibacterial effects of Thymus vulgaris (Lamiaceae), Lavandula angustifolia (Lamiaceae), and Calamintha nepeta (Lamiaceae) Savi subsp. nepeta var. subisodonda (Borb.) Hayek essential oils on five different bacteria were estimated. Laboratory control strain and clinical isolates from different pathogenic media were researched by broth microdilution method, with an emphasis on a chemical composition-antibacterial activity relationship. The main constituents of thyme oil were thymol (59.95%) and p-cymene (18.34%). Linalool acetate (38.23%) and beta-linalool (35.01%) were main compounds in lavender oil. C. nepeta essential oil was characterized by a high percentage of piperitone oxide (59.07%) and limonene (9.05%). Essential oils have been found to have antimicrobial activity against all tested microorganisms. Classification and comparison of essential oils on the basis of their chemical composition and antibacterial activity were made by utilization of appropriate chemometric methods. The chemical principal component analysis (PCA) and hierachical cluster analysis (HCA) separated essential oils into two groups and two sub-groups. Thyme essential oil forms separate chemical HCA group and exhibits highest antibacterial activity, similar to tetracycline. Essential oils of lavender and C. nepeta in the same chemical HCA group were classified in different groups, within antibacterial PCA and HCA analyses. Lavender oil exhibits higher antibacterial ability in comparison with C. nepeta essential oil, probably based on the concept of synergistic activity of essential oil components.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Analytical and Bioanalytical Chemistry",
title = "Investigation of the chemical composition-antibacterial activity relationship of essential oils by chemometric methods",
volume = "403",
number = "4",
pages = "1007-1018",
doi = "10.1007/s00216-012-5866-1"
}

Miladinovic, D. L., Ilic, B. S., Mihajilov-Krstev, T. M., Nikolic, N. D., Miladinovic, L. C.,& Cvetković, O.. (2012). Investigation of the chemical composition-antibacterial activity relationship of essential oils by chemometric methods. in Analytical and Bioanalytical Chemistry
Springer Heidelberg, Heidelberg., 403(4), 1007-1018.
https://doi.org/10.1007/s00216-012-5866-1

Miladinovic DL, Ilic BS, Mihajilov-Krstev TM, Nikolic ND, Miladinovic LC, Cvetković O. Investigation of the chemical composition-antibacterial activity relationship of essential oils by chemometric methods. in Analytical and Bioanalytical Chemistry. 2012;403(4):1007-1018.
doi:10.1007/s00216-012-5866-1 .

Miladinovic, Dragoljub L., Ilic, Budimir S., Mihajilov-Krstev, Tatjana M., Nikolic, Nikola D., Miladinovic, Ljiljana C., Cvetković, Olga, "Investigation of the chemical composition-antibacterial activity relationship of essential oils by chemometric methods" in Analytical and Bioanalytical Chemistry, 403, no. 4 (2012):1007-1018,
https://doi.org/10.1007/s00216-012-5866-1 . .