TY  - JOUR
AU  - Grujic, Marica
AU  - Dojnov, Biljana
AU  - Potocnik, Ivana
AU  - Duduk, Bojan
AU  - Vujčić, Zoran
PY  - 2015
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1673
AB  - Mushroom production is the biggest solid state fermentation industry in the world. Disposal and storage of spent mushroom compost (SMC) that remains after mushroom harvest poses a big economic and environmental problem. Production of industrially important hydrolytic enzymes by fungi on various agro-industrial wastes is a significant, open chapter in biotechnology. This paper proposes a novel application of SMC as substrate for cultivation of fungi in solid state fermentation (SSF) in order to obtain the enzymes cellulase, xylanase, amylase and beta-glucosidase. SMC can be used as a good substrate for cultivation of Trichoderma and Aspergillus without the addition of supplementary (nutritive) elements. Starting amount of SMC was reduced by 30% due to hydrolysis by a complex of cellulolytic enzymes. Material that is left behind is a more suitable fertilizer for horticulture. One fungal isolate was pointed out as a promising producer (Trichoderma atroviride isolate T42). It produced the greatest amount of total protein (0.204 mg mL(-1)), five isoforms of beta-glucosidase and the highest level (12 isoforms) of both endocellulase (0.76 U mL(-1)) and xylanase (2.31 U mL(-1)). The capacity of T42 to produce all examined enzymes in such a high number of isoforms demonstrates successful adaptation to new substrates.
PB  - Elsevier Sci Ltd, Oxford
T2  - International Biodeterioration & Biodegradation
T1  - Spent mushroom compost as substrate for the production of industrially important hydrolytic enzymes by fungi Trichoderma spp. and Aspergillus niger in solid state fermentation
VL  - 104
SP  - 290
EP  - 298
DO  - 10.1016/j.ibiod.2015.04.029
ER  - 

@article{
author = "Grujic, Marica and Dojnov, Biljana and Potocnik, Ivana and Duduk, Bojan and Vujčić, Zoran",
year = "2015",
abstract = "Mushroom production is the biggest solid state fermentation industry in the world. Disposal and storage of spent mushroom compost (SMC) that remains after mushroom harvest poses a big economic and environmental problem. Production of industrially important hydrolytic enzymes by fungi on various agro-industrial wastes is a significant, open chapter in biotechnology. This paper proposes a novel application of SMC as substrate for cultivation of fungi in solid state fermentation (SSF) in order to obtain the enzymes cellulase, xylanase, amylase and beta-glucosidase. SMC can be used as a good substrate for cultivation of Trichoderma and Aspergillus without the addition of supplementary (nutritive) elements. Starting amount of SMC was reduced by 30% due to hydrolysis by a complex of cellulolytic enzymes. Material that is left behind is a more suitable fertilizer for horticulture. One fungal isolate was pointed out as a promising producer (Trichoderma atroviride isolate T42). It produced the greatest amount of total protein (0.204 mg mL(-1)), five isoforms of beta-glucosidase and the highest level (12 isoforms) of both endocellulase (0.76 U mL(-1)) and xylanase (2.31 U mL(-1)). The capacity of T42 to produce all examined enzymes in such a high number of isoforms demonstrates successful adaptation to new substrates.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "International Biodeterioration & Biodegradation",
title = "Spent mushroom compost as substrate for the production of industrially important hydrolytic enzymes by fungi Trichoderma spp. and Aspergillus niger in solid state fermentation",
volume = "104",
pages = "290-298",
doi = "10.1016/j.ibiod.2015.04.029"
}

Grujic, M., Dojnov, B., Potocnik, I., Duduk, B.,& Vujčić, Z.. (2015). Spent mushroom compost as substrate for the production of industrially important hydrolytic enzymes by fungi Trichoderma spp. and Aspergillus niger in solid state fermentation. in International Biodeterioration & Biodegradation
Elsevier Sci Ltd, Oxford., 104, 290-298.
https://doi.org/10.1016/j.ibiod.2015.04.029

Grujic M, Dojnov B, Potocnik I, Duduk B, Vujčić Z. Spent mushroom compost as substrate for the production of industrially important hydrolytic enzymes by fungi Trichoderma spp. and Aspergillus niger in solid state fermentation. in International Biodeterioration & Biodegradation. 2015;104:290-298.
doi:10.1016/j.ibiod.2015.04.029 .

Grujic, Marica, Dojnov, Biljana, Potocnik, Ivana, Duduk, Bojan, Vujčić, Zoran, "Spent mushroom compost as substrate for the production of industrially important hydrolytic enzymes by fungi Trichoderma spp. and Aspergillus niger in solid state fermentation" in International Biodeterioration & Biodegradation, 104 (2015):290-298,
https://doi.org/10.1016/j.ibiod.2015.04.029 . .

TY  - JOUR
AU  - Dojnov, Biljana
AU  - Grujic, Marica
AU  - Vujčić, Zoran
PY  - 2015
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1713
AB  - A method for zymographic detection of specific cellulases in a complex (endocellulase, exocellulase, and cellobiase) from crude fermentation extracts, after a single electrophoretic separation, is described in this paper. Cellulases were printed onto a membrane and, subsequently, substrate gel. Cellobiase isoforms were detected on the membrane using esculine as substrate, endocellulase isoforms on substrate gel with copolymerized carboxymethyl cellulose (CMC), while exocellulase isoforms were detected in electrophoresis gel with 4-methylumbelliferyl--d-cellobioside (MUC). This can be a useful additional tool for monitoring and control of fungal cellulase production in industrial processes and fundamental research, screening for particular cellulase producers, or testing of new lignocellulose substrates.
PB  - Wiley-Blackwell, Hoboken
T2  - Electrophoresis
T1  - Reliable simultaneous zymographic method of characterization of cellulolytic enzymes from fungal cellulase complex
VL  - 36
IS  - 15
SP  - 1724
EP  - 1727
DO  - 10.1002/elps.201400541
ER  - 

@article{
author = "Dojnov, Biljana and Grujic, Marica and Vujčić, Zoran",
year = "2015",
abstract = "A method for zymographic detection of specific cellulases in a complex (endocellulase, exocellulase, and cellobiase) from crude fermentation extracts, after a single electrophoretic separation, is described in this paper. Cellulases were printed onto a membrane and, subsequently, substrate gel. Cellobiase isoforms were detected on the membrane using esculine as substrate, endocellulase isoforms on substrate gel with copolymerized carboxymethyl cellulose (CMC), while exocellulase isoforms were detected in electrophoresis gel with 4-methylumbelliferyl--d-cellobioside (MUC). This can be a useful additional tool for monitoring and control of fungal cellulase production in industrial processes and fundamental research, screening for particular cellulase producers, or testing of new lignocellulose substrates.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Electrophoresis",
title = "Reliable simultaneous zymographic method of characterization of cellulolytic enzymes from fungal cellulase complex",
volume = "36",
number = "15",
pages = "1724-1727",
doi = "10.1002/elps.201400541"
}

Dojnov, B., Grujic, M.,& Vujčić, Z.. (2015). Reliable simultaneous zymographic method of characterization of cellulolytic enzymes from fungal cellulase complex. in Electrophoresis
Wiley-Blackwell, Hoboken., 36(15), 1724-1727.
https://doi.org/10.1002/elps.201400541

Dojnov B, Grujic M, Vujčić Z. Reliable simultaneous zymographic method of characterization of cellulolytic enzymes from fungal cellulase complex. in Electrophoresis. 2015;36(15):1724-1727.
doi:10.1002/elps.201400541 .

Dojnov, Biljana, Grujic, Marica, Vujčić, Zoran, "Reliable simultaneous zymographic method of characterization of cellulolytic enzymes from fungal cellulase complex" in Electrophoresis, 36, no. 15 (2015):1724-1727,
https://doi.org/10.1002/elps.201400541 . .

TY  - JOUR
AU  - Dojnov, Biljana
AU  - Grujic, Marica
AU  - Vujčić, Zoran
PY  - 2015
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1783
AB  - Triticale (x Triticosecale, Wittmack), an important industrial crop, with high grain yield, containing high amounts of starch, proteins and also major and minor mineral elements, is not yet sufficiently utilized. The simultaneous production of alpha-amylase and glucoamylase isoforms by Aspergillus niger on triticale grains, without any nutritive supplements, was developed, optimized and scaled up 10 fold for the first time. The specific combination of the examined effects led to the production of a novel glucoamylase isoform. Reduction of particle size, increase in oxygen availability and substrate height lead to an increase of 30 % in the production of amylases. Reduction of the relative humidity from 65 to 30 % increased glucoamylase production 2 fold and alpha-amylase production by 30%. The peak production of alpha-amylase (158 U g(-1)) and glucoamylase (170 U g(-1)) were obtained in Erlenmeyer flasks and in scaled-up trays. The obtained A. niger amylase cocktail was more efficient in raw starch hydrolysis from wheat flour, 29 % more efficient in glucose formation and 10 % more efficient in total reducing sugar formation, than the commercially available amylase cocktail SAN Super 240L, which is widely used in industry.
PB  - Serbian Chemical Soc, Belgrade
T2  - Journal of the Serbian Chemical Society
T1  - Highly efficient production of Aspergillus niger amylase cocktail by solid-state fermentation using triticale grains as a well-balanced substrate
VL  - 80
IS  - 11
SP  - 1375
EP  - 1390
DO  - 10.2298/JSC150317041D
ER  - 

@article{
author = "Dojnov, Biljana and Grujic, Marica and Vujčić, Zoran",
year = "2015",
abstract = "Triticale (x Triticosecale, Wittmack), an important industrial crop, with high grain yield, containing high amounts of starch, proteins and also major and minor mineral elements, is not yet sufficiently utilized. The simultaneous production of alpha-amylase and glucoamylase isoforms by Aspergillus niger on triticale grains, without any nutritive supplements, was developed, optimized and scaled up 10 fold for the first time. The specific combination of the examined effects led to the production of a novel glucoamylase isoform. Reduction of particle size, increase in oxygen availability and substrate height lead to an increase of 30 % in the production of amylases. Reduction of the relative humidity from 65 to 30 % increased glucoamylase production 2 fold and alpha-amylase production by 30%. The peak production of alpha-amylase (158 U g(-1)) and glucoamylase (170 U g(-1)) were obtained in Erlenmeyer flasks and in scaled-up trays. The obtained A. niger amylase cocktail was more efficient in raw starch hydrolysis from wheat flour, 29 % more efficient in glucose formation and 10 % more efficient in total reducing sugar formation, than the commercially available amylase cocktail SAN Super 240L, which is widely used in industry.",
publisher = "Serbian Chemical Soc, Belgrade",
journal = "Journal of the Serbian Chemical Society",
title = "Highly efficient production of Aspergillus niger amylase cocktail by solid-state fermentation using triticale grains as a well-balanced substrate",
volume = "80",
number = "11",
pages = "1375-1390",
doi = "10.2298/JSC150317041D"
}

Dojnov, B., Grujic, M.,& Vujčić, Z.. (2015). Highly efficient production of Aspergillus niger amylase cocktail by solid-state fermentation using triticale grains as a well-balanced substrate. in Journal of the Serbian Chemical Society
Serbian Chemical Soc, Belgrade., 80(11), 1375-1390.
https://doi.org/10.2298/JSC150317041D

Dojnov B, Grujic M, Vujčić Z. Highly efficient production of Aspergillus niger amylase cocktail by solid-state fermentation using triticale grains as a well-balanced substrate. in Journal of the Serbian Chemical Society. 2015;80(11):1375-1390.
doi:10.2298/JSC150317041D .

Dojnov, Biljana, Grujic, Marica, Vujčić, Zoran, "Highly efficient production of Aspergillus niger amylase cocktail by solid-state fermentation using triticale grains as a well-balanced substrate" in Journal of the Serbian Chemical Society, 80, no. 11 (2015):1375-1390,
https://doi.org/10.2298/JSC150317041D . .

TY  - JOUR
AU  - Dojnov, Biljana
AU  - Grujic, Marica
AU  - Percevic, Bojana
AU  - Vujčić, Zoran
PY  - 2015
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1809
AB  - With the purpose of finding a suitable available inducer in combination with starvation, carbohydrate mixtures from triticale were used and compared with well-known amylase inducers in fungi. Carbohydrate mixtures from triticale induced the production of an amylase cocktail (alpha-amylase and glucoamylase) in Aspergillus niger, unlike induction with well-known inducers that induce only glucoamylase, shown by zymography and TLC analysis of the carbohydrate mixtures before and after fermentation. Glucoamylase production by A. niger was the highest in the presence of the extract obtained after auto-hydrolysis of starch from triticale (95.88 U mL(-1)). Carbohydrate mixtures from triticale induced the production of alpha-amylase in A. oryzae. More alpha-amylase isoforms were detected when using a complex carbohydrate mixture, compared to induction with maltose or starch. A 48-h induction was the most efficient using a triticale extract (101.35 U mL(-1)). Carbohydrates from triticale extracts could be used as very good cheap amylase inducers. Triticale, still not fully utilized, could be taken into consideration as an inducer in amylase production by Aspergillus sp, and in such a way, it could be used as the sole substrate in fermentation.
PB  - Serbian Chemical Soc, Belgrade
T2  - Journal of the Serbian Chemical Society
T1  - Enhancement of amylase production by Aspergillus sp using carbohydrates mixtures from triticale
VL  - 80
IS  - 10
SP  - 1279
EP  - 1288
DO  - 10.2298/JSC150317043D
ER  - 

@article{
author = "Dojnov, Biljana and Grujic, Marica and Percevic, Bojana and Vujčić, Zoran",
year = "2015",
abstract = "With the purpose of finding a suitable available inducer in combination with starvation, carbohydrate mixtures from triticale were used and compared with well-known amylase inducers in fungi. Carbohydrate mixtures from triticale induced the production of an amylase cocktail (alpha-amylase and glucoamylase) in Aspergillus niger, unlike induction with well-known inducers that induce only glucoamylase, shown by zymography and TLC analysis of the carbohydrate mixtures before and after fermentation. Glucoamylase production by A. niger was the highest in the presence of the extract obtained after auto-hydrolysis of starch from triticale (95.88 U mL(-1)). Carbohydrate mixtures from triticale induced the production of alpha-amylase in A. oryzae. More alpha-amylase isoforms were detected when using a complex carbohydrate mixture, compared to induction with maltose or starch. A 48-h induction was the most efficient using a triticale extract (101.35 U mL(-1)). Carbohydrates from triticale extracts could be used as very good cheap amylase inducers. Triticale, still not fully utilized, could be taken into consideration as an inducer in amylase production by Aspergillus sp, and in such a way, it could be used as the sole substrate in fermentation.",
publisher = "Serbian Chemical Soc, Belgrade",
journal = "Journal of the Serbian Chemical Society",
title = "Enhancement of amylase production by Aspergillus sp using carbohydrates mixtures from triticale",
volume = "80",
number = "10",
pages = "1279-1288",
doi = "10.2298/JSC150317043D"
}

Dojnov, B., Grujic, M., Percevic, B.,& Vujčić, Z.. (2015). Enhancement of amylase production by Aspergillus sp using carbohydrates mixtures from triticale. in Journal of the Serbian Chemical Society
Serbian Chemical Soc, Belgrade., 80(10), 1279-1288.
https://doi.org/10.2298/JSC150317043D

Dojnov B, Grujic M, Percevic B, Vujčić Z. Enhancement of amylase production by Aspergillus sp using carbohydrates mixtures from triticale. in Journal of the Serbian Chemical Society. 2015;80(10):1279-1288.
doi:10.2298/JSC150317043D .

Dojnov, Biljana, Grujic, Marica, Percevic, Bojana, Vujčić, Zoran, "Enhancement of amylase production by Aspergillus sp using carbohydrates mixtures from triticale" in Journal of the Serbian Chemical Society, 80, no. 10 (2015):1279-1288,
https://doi.org/10.2298/JSC150317043D . .

TY  - JOUR
AU  - Pavlovic, R.
AU  - Grujic, Marica
AU  - Dojnov, Biljana
AU  - Vujčić, Miroslava
AU  - Nenadovic, Vera
AU  - Ivanovic, Jelisaveta
AU  - Vujčić, Zoran
PY  - 2012
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/943
AB  - The expression and distribution of digestive cellulases along the midgut of Cerambyx cerdo larvae were analyzed for the first time and are presented in this article. Four groups of larvae were examined: larvae developed in the wild; larvae taken from the wild and successively reared on an artificial diet based on polenta; and larvae hatched in the laboratory and reared on two different artificial diets. Seven endocellulase and seven β-D-glucosidase isoforms were detected in all midgut extracts of C. cerdo with a zymogram after native PAGE. We observed that C. cerdo larvae are capable of producing cellulase isoforms with different PAGE mobilities depending on the nutrient substrate. From our findings it can be assumed that, depending on the distribution of endocellulase and β-D-glucosidase, cellulose molecules are first fragmented in the anterior and middle midgut by endo-β-1,4-glucanase; subsequently, the obtained fragments are broken down by β-D-glucosidase mostly in middle midgut.
PB  - Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd
T2  - Archives of Biological Sciences
T1  - Influence of nutrient substrates on the expression of cellulases in Cerambyx cerdo L. (Coleoptera: Cerambycidae) larvae
VL  - 64
IS  - 2
SP  - 757
EP  - 765
DO  - 10.2298/ABS1202757P
ER  - 

@article{
author = "Pavlovic, R. and Grujic, Marica and Dojnov, Biljana and Vujčić, Miroslava and Nenadovic, Vera and Ivanovic, Jelisaveta and Vujčić, Zoran",
year = "2012",
abstract = "The expression and distribution of digestive cellulases along the midgut of Cerambyx cerdo larvae were analyzed for the first time and are presented in this article. Four groups of larvae were examined: larvae developed in the wild; larvae taken from the wild and successively reared on an artificial diet based on polenta; and larvae hatched in the laboratory and reared on two different artificial diets. Seven endocellulase and seven β-D-glucosidase isoforms were detected in all midgut extracts of C. cerdo with a zymogram after native PAGE. We observed that C. cerdo larvae are capable of producing cellulase isoforms with different PAGE mobilities depending on the nutrient substrate. From our findings it can be assumed that, depending on the distribution of endocellulase and β-D-glucosidase, cellulose molecules are first fragmented in the anterior and middle midgut by endo-β-1,4-glucanase; subsequently, the obtained fragments are broken down by β-D-glucosidase mostly in middle midgut.",
publisher = "Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd",
journal = "Archives of Biological Sciences",
title = "Influence of nutrient substrates on the expression of cellulases in Cerambyx cerdo L. (Coleoptera: Cerambycidae) larvae",
volume = "64",
number = "2",
pages = "757-765",
doi = "10.2298/ABS1202757P"
}

Pavlovic, R., Grujic, M., Dojnov, B., Vujčić, M., Nenadovic, V., Ivanovic, J.,& Vujčić, Z.. (2012). Influence of nutrient substrates on the expression of cellulases in Cerambyx cerdo L. (Coleoptera: Cerambycidae) larvae. in Archives of Biological Sciences
Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd., 64(2), 757-765.
https://doi.org/10.2298/ABS1202757P

Pavlovic R, Grujic M, Dojnov B, Vujčić M, Nenadovic V, Ivanovic J, Vujčić Z. Influence of nutrient substrates on the expression of cellulases in Cerambyx cerdo L. (Coleoptera: Cerambycidae) larvae. in Archives of Biological Sciences. 2012;64(2):757-765.
doi:10.2298/ABS1202757P .

Pavlovic, R., Grujic, Marica, Dojnov, Biljana, Vujčić, Miroslava, Nenadovic, Vera, Ivanovic, Jelisaveta, Vujčić, Zoran, "Influence of nutrient substrates on the expression of cellulases in Cerambyx cerdo L. (Coleoptera: Cerambycidae) larvae" in Archives of Biological Sciences, 64, no. 2 (2012):757-765,
https://doi.org/10.2298/ABS1202757P . .