TY  - CONF
AU  - Krizmanić, Jelena
AU  - Stupar, Miloš
AU  - Ljaljević Grbić, Milica
AU  - Dimitrijević, Jelica
AU  - Vidaković, Danijela
AU  - Stamenković, Srđan
AU  - Krizmanić, Imre
AU  - Breka, Katarina
PY  - 2019
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4622
AB  - Cutaneous microbiota of amphibians is considered
to be an indicator of the health status of examined
individuals, as well as of environmental factors. In
that sense the skin of the captured individuals
belonging to Pelophylax esculenta complex on the
locality Stevanove ravnice within the Special Nature
Reserve “Deliblato Sand” (Serbia) was screened for
the presence of biofilm-forming cyanobacteria,
algae and fungi dwelling directly on frogs’ stratum
corneum. Biofilm was sampled using non-aggressive
“adhesive tape method” and their constituents were
analyzed and characterized microscopically (Carl
Zeiss AxioImager.M1 with AxioVision 4.9 software).
We detected high diversity and abundance
of diatom taxa overall, particularly belonging to
genera Navicula, Gomphonema, Geissleria,
Placoneis and Nitzschia. Cyanobacteria were less
present and with low abundance. Among them
chroococcal genera Gloeocapsopsis, Pseudocapsa
and Aphanocapsa were recorded. Outside of diatoms
and cyanobacteria, genus Chlorella was the
only present photoautotrophic biofilm constituent.
We consider highly mucous green frogs skin as
a favorable environment for the survival of diatoms
and cyanobacteria. Light microscopy analyses of
biofilm fragments revealed the presence of variety
of fungal structures. Documented coenocytic mycelium
with zoosporangium, originated presumably
from oomycetes, as well as septate melanized
hyphae of dematiaceous fungi, suggested colonization
of frog skin from both types of environments
present, water and surrounding vegetation.
A variety of fungal spores (fragmospores, amerospores,
dictyospores, didymospores), and in few
cases spore germination were recorded on frog
skin. Detected biofilm composition was not determinate
by species and gender-specific.
PB  - Taylor & Francis
C3  - European Journal of Phycology (Posters)
T1  - Green frogs skin harbours microbiota treasure
VL  - 54
IS  - sup1
SP  - 156
EP  - 156
DO  - 10.1080/09670262.2019.1626628
ER  - 

@conference{
author = "Krizmanić, Jelena and Stupar, Miloš and Ljaljević Grbić, Milica and Dimitrijević, Jelica and Vidaković, Danijela and Stamenković, Srđan and Krizmanić, Imre and Breka, Katarina",
year = "2019",
abstract = "Cutaneous microbiota of amphibians is considered
to be an indicator of the health status of examined
individuals, as well as of environmental factors. In
that sense the skin of the captured individuals
belonging to Pelophylax esculenta complex on the
locality Stevanove ravnice within the Special Nature
Reserve “Deliblato Sand” (Serbia) was screened for
the presence of biofilm-forming cyanobacteria,
algae and fungi dwelling directly on frogs’ stratum
corneum. Biofilm was sampled using non-aggressive
“adhesive tape method” and their constituents were
analyzed and characterized microscopically (Carl
Zeiss AxioImager.M1 with AxioVision 4.9 software).
We detected high diversity and abundance
of diatom taxa overall, particularly belonging to
genera Navicula, Gomphonema, Geissleria,
Placoneis and Nitzschia. Cyanobacteria were less
present and with low abundance. Among them
chroococcal genera Gloeocapsopsis, Pseudocapsa
and Aphanocapsa were recorded. Outside of diatoms
and cyanobacteria, genus Chlorella was the
only present photoautotrophic biofilm constituent.
We consider highly mucous green frogs skin as
a favorable environment for the survival of diatoms
and cyanobacteria. Light microscopy analyses of
biofilm fragments revealed the presence of variety
of fungal structures. Documented coenocytic mycelium
with zoosporangium, originated presumably
from oomycetes, as well as septate melanized
hyphae of dematiaceous fungi, suggested colonization
of frog skin from both types of environments
present, water and surrounding vegetation.
A variety of fungal spores (fragmospores, amerospores,
dictyospores, didymospores), and in few
cases spore germination were recorded on frog
skin. Detected biofilm composition was not determinate
by species and gender-specific.",
publisher = "Taylor & Francis",
journal = "European Journal of Phycology (Posters)",
title = "Green frogs skin harbours microbiota treasure",
volume = "54",
number = "sup1",
pages = "156-156",
doi = "10.1080/09670262.2019.1626628"
}

Krizmanić, J., Stupar, M., Ljaljević Grbić, M., Dimitrijević, J., Vidaković, D., Stamenković, S., Krizmanić, I.,& Breka, K.. (2019). Green frogs skin harbours microbiota treasure. in European Journal of Phycology (Posters)
Taylor & Francis., 54(sup1), 156-156.
https://doi.org/10.1080/09670262.2019.1626628

Krizmanić J, Stupar M, Ljaljević Grbić M, Dimitrijević J, Vidaković D, Stamenković S, Krizmanić I, Breka K. Green frogs skin harbours microbiota treasure. in European Journal of Phycology (Posters). 2019;54(sup1):156-156.
doi:10.1080/09670262.2019.1626628 .

Krizmanić, Jelena, Stupar, Miloš, Ljaljević Grbić, Milica, Dimitrijević, Jelica, Vidaković, Danijela, Stamenković, Srđan, Krizmanić, Imre, Breka, Katarina, "Green frogs skin harbours microbiota treasure" in European Journal of Phycology (Posters), 54, no. sup1 (2019):156-156,
https://doi.org/10.1080/09670262.2019.1626628 . .

TY  - CONF
AU  - Vidaković, Danijela
AU  - Dimitrijević, Jelica
AU  - Krizmanić, Imre
AU  - Krizmanić, Jelena
PY  - 2018
UR  - http://botanicaserbica.bio.bg.ac.rs/arhiva/pdf/2018_42_2_1_full.pdf
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4649
AB  - Epibiotic diatoms inhabiting the carapace of European pond turtle (Emys orbicularis Linnaeus, 1758) are examined between May and July 2017 from three locations in Vojvodina (Serbia): Special nature reserve „Deliblatska Peščara“, Natural monument „Ivankovačka ada“ and Special nature reserve „Gornje podunavlje“. Our objective was to compare the diatom flora on a single turtle species across part of its range. Diatoms were removed by brushing from the carapaces, preserved in 4% formalin and identified under Carl Zeiss AxioImager.M1 microscope after preparing permanent slides with Naphrax medium. The diverse diatom assemblages were recorded. A total of 116 diatom taxa from 32 genera were found on the carapace of European pond turtle. The highest diversity in epibiotic diatom community was recorded at SNP „Deliblatska Peščara“ and NM „Ivankovačka ada“ (85 and 81 taxa, respectively). Only 6 diatom taxa (Nitzschia denticula, N. fonticola, Fragilaria ulna, Cymbella parva, C. perparva and Cyclotella sp.) were found on all three localities. Of these species, Cymbella perparva i Nitzschia denticula were occurred on the greatest number of turtles. In contrast, 67 diatom taxa occurred only on a single locality. Diatom richness and abundance were variable both among and within localities. The most numerous were erect (Cymbella spp., Gomphonema spp.) and motile (Nitzschia spp., Navicula spp.) forms.
PB  - Institute of Botany and Botanical Garden 'Jevremovac'
C3  - Botanica Serbica
T1  - Diatoms on the carapace of European pond turtle (Emys orbicularis L.) in Vojvodina
VL  - 48
IS  - sup 1
SP  - 204
EP  - 204
UR  - https://hdl.handle.net/21.15107/rcub_cer_4649
ER  - 

@conference{
author = "Vidaković, Danijela and Dimitrijević, Jelica and Krizmanić, Imre and Krizmanić, Jelena",
year = "2018",
abstract = "Epibiotic diatoms inhabiting the carapace of European pond turtle (Emys orbicularis Linnaeus, 1758) are examined between May and July 2017 from three locations in Vojvodina (Serbia): Special nature reserve „Deliblatska Peščara“, Natural monument „Ivankovačka ada“ and Special nature reserve „Gornje podunavlje“. Our objective was to compare the diatom flora on a single turtle species across part of its range. Diatoms were removed by brushing from the carapaces, preserved in 4% formalin and identified under Carl Zeiss AxioImager.M1 microscope after preparing permanent slides with Naphrax medium. The diverse diatom assemblages were recorded. A total of 116 diatom taxa from 32 genera were found on the carapace of European pond turtle. The highest diversity in epibiotic diatom community was recorded at SNP „Deliblatska Peščara“ and NM „Ivankovačka ada“ (85 and 81 taxa, respectively). Only 6 diatom taxa (Nitzschia denticula, N. fonticola, Fragilaria ulna, Cymbella parva, C. perparva and Cyclotella sp.) were found on all three localities. Of these species, Cymbella perparva i Nitzschia denticula were occurred on the greatest number of turtles. In contrast, 67 diatom taxa occurred only on a single locality. Diatom richness and abundance were variable both among and within localities. The most numerous were erect (Cymbella spp., Gomphonema spp.) and motile (Nitzschia spp., Navicula spp.) forms.",
publisher = "Institute of Botany and Botanical Garden 'Jevremovac'",
journal = "Botanica Serbica",
title = "Diatoms on the carapace of European pond turtle (Emys orbicularis L.) in Vojvodina",
volume = "48",
number = "sup 1",
pages = "204-204",
url = "https://hdl.handle.net/21.15107/rcub_cer_4649"
}

Vidaković, D., Dimitrijević, J., Krizmanić, I.,& Krizmanić, J.. (2018). Diatoms on the carapace of European pond turtle (Emys orbicularis L.) in Vojvodina. in Botanica Serbica
Institute of Botany and Botanical Garden 'Jevremovac'., 48(sup 1), 204-204.
https://hdl.handle.net/21.15107/rcub_cer_4649

Vidaković D, Dimitrijević J, Krizmanić I, Krizmanić J. Diatoms on the carapace of European pond turtle (Emys orbicularis L.) in Vojvodina. in Botanica Serbica. 2018;48(sup 1):204-204.
https://hdl.handle.net/21.15107/rcub_cer_4649 .

Vidaković, Danijela, Dimitrijević, Jelica, Krizmanić, Imre, Krizmanić, Jelena, "Diatoms on the carapace of European pond turtle (Emys orbicularis L.) in Vojvodina" in Botanica Serbica, 48, no. sup 1 (2018):204-204,
https://hdl.handle.net/21.15107/rcub_cer_4649 .