TY  - JOUR
AU  - Jugović, Branimir
AU  - Grgur, Branimir N.
AU  - Antov, Mirjana
AU  - Knežević-Jugović, Zorica
AU  - Stevanović, Jasmina
AU  - Gvozdenović, Milica M.
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1962
AB  - Polypyrrole enzyme electrode was formed by immobilization of glucose oxidase via glutaraldehyde into electrochemically synthesized polypyrrole on glassy carbon electrode. Electrochemical synthesis was performed in 0.5 mol dm(-3) HCl and 0.2 mol dm(-3) pyrrole at constant current density of 2 mA cm(-2). Chronopotentiometric curves of polypyrrole enzyme electrode were recorded at current density of 42 nA cm(-2) for different glucose concentrations. The determined value of the apparent Michaelis-Menten constant was 0.045 mmol dm(-3) which is significantly lower than that of free enzyme indicating enhanced enzyme efficiency when it is immobilized into electroconducting polymer matrix.
PB  - Esg, Belgrade
T2  - International Journal of Electrochemical Science
T1  - Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose
VL  - 11
IS  - 2
SP  - 1152
EP  - 1161
UR  - https://hdl.handle.net/21.15107/rcub_dais_15979
ER  - 

@article{
author = "Jugović, Branimir and Grgur, Branimir N. and Antov, Mirjana and Knežević-Jugović, Zorica and Stevanović, Jasmina and Gvozdenović, Milica M.",
year = "2016",
abstract = "Polypyrrole enzyme electrode was formed by immobilization of glucose oxidase via glutaraldehyde into electrochemically synthesized polypyrrole on glassy carbon electrode. Electrochemical synthesis was performed in 0.5 mol dm(-3) HCl and 0.2 mol dm(-3) pyrrole at constant current density of 2 mA cm(-2). Chronopotentiometric curves of polypyrrole enzyme electrode were recorded at current density of 42 nA cm(-2) for different glucose concentrations. The determined value of the apparent Michaelis-Menten constant was 0.045 mmol dm(-3) which is significantly lower than that of free enzyme indicating enhanced enzyme efficiency when it is immobilized into electroconducting polymer matrix.",
publisher = "Esg, Belgrade",
journal = "International Journal of Electrochemical Science",
title = "Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose",
volume = "11",
number = "2",
pages = "1152-1161",
url = "https://hdl.handle.net/21.15107/rcub_dais_15979"
}

Jugović, B., Grgur, B. N., Antov, M., Knežević-Jugović, Z., Stevanović, J.,& Gvozdenović, M. M.. (2016). Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose. in International Journal of Electrochemical Science
Esg, Belgrade., 11(2), 1152-1161.
https://hdl.handle.net/21.15107/rcub_dais_15979

Jugović B, Grgur BN, Antov M, Knežević-Jugović Z, Stevanović J, Gvozdenović MM. Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose. in International Journal of Electrochemical Science. 2016;11(2):1152-1161.
https://hdl.handle.net/21.15107/rcub_dais_15979 .

Jugović, Branimir, Grgur, Branimir N., Antov, Mirjana, Knežević-Jugović, Zorica, Stevanović, Jasmina, Gvozdenović, Milica M., "Polypyrrole-based Enzyme Electrode with Immobilized Glucose Oxidase for Electrochemical Determination of Glucose" in International Journal of Electrochemical Science, 11, no. 2 (2016):1152-1161,
https://hdl.handle.net/21.15107/rcub_dais_15979 .

TY  - JOUR
AU  - Jambrec, Daliborka
AU  - Gvozdenović, Milica M.
AU  - Antov, Mirjana
AU  - Grgur, Branimir N.
AU  - Jokić, Bojan
AU  - Stevanović, Jasmina
AU  - Jugović, Branimir
PY  - 2012
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/975
AB  - Electrochemical deposition of polyaniline (PANI) on graphite electrode was performed galvanostaticaly at constant current density in the range of 1.0 - 5.0 mA cm(-2) from aqueous acidic electrolyte containing aniline monomer. Based on ratio of doping/dedoping charge capacities, it was estimated that current density of 2.0 mA cm(-2) was optimal. The structure of the electrochemically synthesized PANI was fibrous, uniform and three dimensional with highly developed surface. Immobilization of glucose oxidise (GOx) was achieved by cross linking via glutaraldehyde and the efficiency of the immobilization was determined spectrophotometrically. Chronoamperometric curves were recorded at different glucose concentrations and used to estimate the apparent Michaelis constant, which was shown to be 0.27 mM. The storage stability of the PANI enzyme electrode was also estimated.
PB  - Inst Materials Physics, Bucharest
T2  - Digest Journal of Nanomaterials and Biostructures
T1  - Electrochemically deposited nano fibrous polyanilne for amperometric determination of glucose
VL  - 7
IS  - 2
SP  - 785
EP  - 794
UR  - https://hdl.handle.net/21.15107/rcub_dais_469
ER  - 

@article{
author = "Jambrec, Daliborka and Gvozdenović, Milica M. and Antov, Mirjana and Grgur, Branimir N. and Jokić, Bojan and Stevanović, Jasmina and Jugović, Branimir",
year = "2012",
abstract = "Electrochemical deposition of polyaniline (PANI) on graphite electrode was performed galvanostaticaly at constant current density in the range of 1.0 - 5.0 mA cm(-2) from aqueous acidic electrolyte containing aniline monomer. Based on ratio of doping/dedoping charge capacities, it was estimated that current density of 2.0 mA cm(-2) was optimal. The structure of the electrochemically synthesized PANI was fibrous, uniform and three dimensional with highly developed surface. Immobilization of glucose oxidise (GOx) was achieved by cross linking via glutaraldehyde and the efficiency of the immobilization was determined spectrophotometrically. Chronoamperometric curves were recorded at different glucose concentrations and used to estimate the apparent Michaelis constant, which was shown to be 0.27 mM. The storage stability of the PANI enzyme electrode was also estimated.",
publisher = "Inst Materials Physics, Bucharest",
journal = "Digest Journal of Nanomaterials and Biostructures",
title = "Electrochemically deposited nano fibrous polyanilne for amperometric determination of glucose",
volume = "7",
number = "2",
pages = "785-794",
url = "https://hdl.handle.net/21.15107/rcub_dais_469"
}

Jambrec, D., Gvozdenović, M. M., Antov, M., Grgur, B. N., Jokić, B., Stevanović, J.,& Jugović, B.. (2012). Electrochemically deposited nano fibrous polyanilne for amperometric determination of glucose. in Digest Journal of Nanomaterials and Biostructures
Inst Materials Physics, Bucharest., 7(2), 785-794.
https://hdl.handle.net/21.15107/rcub_dais_469

Jambrec D, Gvozdenović MM, Antov M, Grgur BN, Jokić B, Stevanović J, Jugović B. Electrochemically deposited nano fibrous polyanilne for amperometric determination of glucose. in Digest Journal of Nanomaterials and Biostructures. 2012;7(2):785-794.
https://hdl.handle.net/21.15107/rcub_dais_469 .

Jambrec, Daliborka, Gvozdenović, Milica M., Antov, Mirjana, Grgur, Branimir N., Jokić, Bojan, Stevanović, Jasmina, Jugović, Branimir, "Electrochemically deposited nano fibrous polyanilne for amperometric determination of glucose" in Digest Journal of Nanomaterials and Biostructures, 7, no. 2 (2012):785-794,
https://hdl.handle.net/21.15107/rcub_dais_469 .