TY  - CONF
AU  - Miljuš, Goran
AU  - Penezić, Ana
AU  - Noe, Dragana
AU  - Dobrijević, Zorana
AU  - Baralić, Marko
AU  - Robajac, Dragana
AU  - Šunderić, Miloš
AU  - Gligorijević, Nikola
AU  - Dimitrijević, Ivan
AU  - Barišić, Goran
AU  - Nedić, Olgica
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6664
AB  - Human serum tansferrin (hsTf) is a major circulatory protein crucial for the
transport/metabolism of Fe3+ ions. By sequestering and delivering ferric ions to target
tissues/cells hsTf maintains redox homeostasis. Oxidative stress (OS), one of the hallmarks
of (patho)physiological conditions, alters protein structure and function. The main role of
hsTf hinges on specific interaction with cellular Tf receptor (TfR) while other interactions
contribute to diverse functions. The aim of this study was to profile interacting partners of
hsTf in the samples of serum coming from patients diagnosed with a wide range of
pathological conditions with underlying OS status. Anti-hsTf pull-down samples were
analysed using mass spectrometry. Data went through analysis by appropriate
bioinformatic tools. Results reveal differences in expression of hsTf interacting proteins in
sample groups of patients suffering from kidney insufficiency subjected to dialysis
treatment (peritoneal-PD or hemo-HD) also with patients with gestational diabetes
compared to respective healthy sample groups. Colorectal cancer stage T3 versus T2 stage
shows an inverse distribution of expression profiles in comparison to healthy samples.
Most prominent differences are seen in hsTf interacting partners involved in the
complement and coagulation cascades and cholesterol metabolic pathways, suggesting a
multifaceted role of hsTf in these processes throughout the course of the disease.
PB  - Serbian Biochemical Society
C3  - Serbian Biochemical Society Twelfth Conference, International scientific meeting, “Biochemistry in Biotechnology,” September 21-23, 2023, Belgrade, Serbia
T1  - Proteomic profiling of anti-transferrin pull-down in patients with underlying oxidative stress
SP  - 69
EP  - 69
UR  - https://hdl.handle.net/21.15107/rcub_cer_6664
ER  - 

@conference{
author = "Miljuš, Goran and Penezić, Ana and Noe, Dragana and Dobrijević, Zorana and Baralić, Marko and Robajac, Dragana and Šunderić, Miloš and Gligorijević, Nikola and Dimitrijević, Ivan and Barišić, Goran and Nedić, Olgica",
year = "2023",
abstract = "Human serum tansferrin (hsTf) is a major circulatory protein crucial for the
transport/metabolism of Fe3+ ions. By sequestering and delivering ferric ions to target
tissues/cells hsTf maintains redox homeostasis. Oxidative stress (OS), one of the hallmarks
of (patho)physiological conditions, alters protein structure and function. The main role of
hsTf hinges on specific interaction with cellular Tf receptor (TfR) while other interactions
contribute to diverse functions. The aim of this study was to profile interacting partners of
hsTf in the samples of serum coming from patients diagnosed with a wide range of
pathological conditions with underlying OS status. Anti-hsTf pull-down samples were
analysed using mass spectrometry. Data went through analysis by appropriate
bioinformatic tools. Results reveal differences in expression of hsTf interacting proteins in
sample groups of patients suffering from kidney insufficiency subjected to dialysis
treatment (peritoneal-PD or hemo-HD) also with patients with gestational diabetes
compared to respective healthy sample groups. Colorectal cancer stage T3 versus T2 stage
shows an inverse distribution of expression profiles in comparison to healthy samples.
Most prominent differences are seen in hsTf interacting partners involved in the
complement and coagulation cascades and cholesterol metabolic pathways, suggesting a
multifaceted role of hsTf in these processes throughout the course of the disease.",
publisher = "Serbian Biochemical Society",
journal = "Serbian Biochemical Society Twelfth Conference, International scientific meeting, “Biochemistry in Biotechnology,” September 21-23, 2023, Belgrade, Serbia",
title = "Proteomic profiling of anti-transferrin pull-down in patients with underlying oxidative stress",
pages = "69-69",
url = "https://hdl.handle.net/21.15107/rcub_cer_6664"
}

Miljuš, G., Penezić, A., Noe, D., Dobrijević, Z., Baralić, M., Robajac, D., Šunderić, M., Gligorijević, N., Dimitrijević, I., Barišić, G.,& Nedić, O.. (2023). Proteomic profiling of anti-transferrin pull-down in patients with underlying oxidative stress. in Serbian Biochemical Society Twelfth Conference, International scientific meeting, “Biochemistry in Biotechnology,” September 21-23, 2023, Belgrade, Serbia
Serbian Biochemical Society., 69-69.
https://hdl.handle.net/21.15107/rcub_cer_6664

Miljuš G, Penezić A, Noe D, Dobrijević Z, Baralić M, Robajac D, Šunderić M, Gligorijević N, Dimitrijević I, Barišić G, Nedić O. Proteomic profiling of anti-transferrin pull-down in patients with underlying oxidative stress. in Serbian Biochemical Society Twelfth Conference, International scientific meeting, “Biochemistry in Biotechnology,” September 21-23, 2023, Belgrade, Serbia. 2023;:69-69.
https://hdl.handle.net/21.15107/rcub_cer_6664 .

Miljuš, Goran, Penezić, Ana, Noe, Dragana, Dobrijević, Zorana, Baralić, Marko, Robajac, Dragana, Šunderić, Miloš, Gligorijević, Nikola, Dimitrijević, Ivan, Barišić, Goran, Nedić, Olgica, "Proteomic profiling of anti-transferrin pull-down in patients with underlying oxidative stress" in Serbian Biochemical Society Twelfth Conference, International scientific meeting, “Biochemistry in Biotechnology,” September 21-23, 2023, Belgrade, Serbia (2023):69-69,
https://hdl.handle.net/21.15107/rcub_cer_6664 .

TY  - JOUR
AU  - Baralić, Marko
AU  - Robajac, Dragana
AU  - Penezić, Ana
AU  - Brković, Voin
AU  - Gligorijević, Nikola
AU  - Bontić, Ana
AU  - Pavlović, Jelena
AU  - Nikolić, Jelena
AU  - Miljuš, Goran
AU  - Dobrijević, Zorana
AU  - Šunderić, Miloš
AU  - Pažitna, Lucija
AU  - Katrlík, Jaroslav
AU  - Nedić, Olgica
AU  - Laušević, Mirjana
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6445
AB  - In previous publications, we pointed out the importance of mannosylation of fibrinogen for the development of cardiovascular complications and fucosylation as a predictor of peritoneal membrane dysfunction in patients on peritoneal dialysis (PD). After a follow-up period of 30 months from the onset of the COVID-19 pandemic, we evaluated the significance of 1,25-dihydroxyvitamin D3 (calcitriol) therapy, primary disease, biochemical and hematologic analyzes, and previously performed glycan analysis by lectin-based microarray as predictors of mortality in this patient group. After univariate Cox regression analysis, diabetes mellitus (DM) and calcitriol therapy were found to be potential predictors of mortality. Additional multivariate Cox regression analysis confirmed that only DM was a predictor of mortality. Nevertheless, the use of calcitriol in therapy significantly reduced mortality in this patient group, as shown by the Kaplan–Meier survival curve. The presence of DM as a concomitant disease proved to be a strong predictor of fatal outcome in PD patients infected with SARS-CoV-2. This is the first study to indicate the importance and beneficial effect of calcitriol therapy on survival in PD patients with COVID-19 infection. In addition, this study points to the possibility that adverse thrombogenic events observed in PD patients during the pandemic may be caused by aberrant fibrinogen glycosylation.
PB  - Switzerland : Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Nutrients
T1  - Significance of 1,25-Dihydroxyvitamin D3 on Overall Mortality in Peritoneal Dialysis Patients with COVID-19
VL  - 15
IS  - 9
SP  - 2050
DO  - 10.3390/nu15092050
ER  - 

@article{
author = "Baralić, Marko and Robajac, Dragana and Penezić, Ana and Brković, Voin and Gligorijević, Nikola and Bontić, Ana and Pavlović, Jelena and Nikolić, Jelena and Miljuš, Goran and Dobrijević, Zorana and Šunderić, Miloš and Pažitna, Lucija and Katrlík, Jaroslav and Nedić, Olgica and Laušević, Mirjana",
year = "2023",
abstract = "In previous publications, we pointed out the importance of mannosylation of fibrinogen for the development of cardiovascular complications and fucosylation as a predictor of peritoneal membrane dysfunction in patients on peritoneal dialysis (PD). After a follow-up period of 30 months from the onset of the COVID-19 pandemic, we evaluated the significance of 1,25-dihydroxyvitamin D3 (calcitriol) therapy, primary disease, biochemical and hematologic analyzes, and previously performed glycan analysis by lectin-based microarray as predictors of mortality in this patient group. After univariate Cox regression analysis, diabetes mellitus (DM) and calcitriol therapy were found to be potential predictors of mortality. Additional multivariate Cox regression analysis confirmed that only DM was a predictor of mortality. Nevertheless, the use of calcitriol in therapy significantly reduced mortality in this patient group, as shown by the Kaplan–Meier survival curve. The presence of DM as a concomitant disease proved to be a strong predictor of fatal outcome in PD patients infected with SARS-CoV-2. This is the first study to indicate the importance and beneficial effect of calcitriol therapy on survival in PD patients with COVID-19 infection. In addition, this study points to the possibility that adverse thrombogenic events observed in PD patients during the pandemic may be caused by aberrant fibrinogen glycosylation.",
publisher = "Switzerland : Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Nutrients",
title = "Significance of 1,25-Dihydroxyvitamin D3 on Overall Mortality in Peritoneal Dialysis Patients with COVID-19",
volume = "15",
number = "9",
pages = "2050",
doi = "10.3390/nu15092050"
}

Baralić, M., Robajac, D., Penezić, A., Brković, V., Gligorijević, N., Bontić, A., Pavlović, J., Nikolić, J., Miljuš, G., Dobrijević, Z., Šunderić, M., Pažitna, L., Katrlík, J., Nedić, O.,& Laušević, M.. (2023). Significance of 1,25-Dihydroxyvitamin D3 on Overall Mortality in Peritoneal Dialysis Patients with COVID-19. in Nutrients
Switzerland : Multidisciplinary Digital Publishing Institute (MDPI)., 15(9), 2050.
https://doi.org/10.3390/nu15092050

Baralić M, Robajac D, Penezić A, Brković V, Gligorijević N, Bontić A, Pavlović J, Nikolić J, Miljuš G, Dobrijević Z, Šunderić M, Pažitna L, Katrlík J, Nedić O, Laušević M. Significance of 1,25-Dihydroxyvitamin D3 on Overall Mortality in Peritoneal Dialysis Patients with COVID-19. in Nutrients. 2023;15(9):2050.
doi:10.3390/nu15092050 .

Baralić, Marko, Robajac, Dragana, Penezić, Ana, Brković, Voin, Gligorijević, Nikola, Bontić, Ana, Pavlović, Jelena, Nikolić, Jelena, Miljuš, Goran, Dobrijević, Zorana, Šunderić, Miloš, Pažitna, Lucija, Katrlík, Jaroslav, Nedić, Olgica, Laušević, Mirjana, "Significance of 1,25-Dihydroxyvitamin D3 on Overall Mortality in Peritoneal Dialysis Patients with COVID-19" in Nutrients, 15, no. 9 (2023):2050,
https://doi.org/10.3390/nu15092050 . .

TY  - JOUR
AU  - Baralić, Marko
AU  - Pažitná, Lucia
AU  - Brković, Voin
AU  - Laušević, Mirjana
AU  - Gligorijević, Nikola
AU  - Katrlík, Jaroslav
AU  - Nedić, Olgica
AU  - Robajac, Dragana
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5664
AB  - As we already reported, fibrinogen fucosylation emerged as a prognostic marker of peritoneal
membrane function in end-stage renal disease (ESRD) patients on peritoneal dialysis. After a
follow-up period of 18 months, we estimated the ability of employed lectins, as well as other biochemical
parameters, to serve as mortality predictors in these patients. Following a univariate Cox
regression analysis, ferritin, urea clearance, residual diuresis, hyperglycemia, and an increase in the
signal intensity obtained with Galanthus nivalis lectin (GNL) emerged as potential mortality predictors,
but additional multivariate Cox regression analysis pointed only to glucose concentration
and GNL as mortality predictors. Higher signal intensity obtained with GNL in patients that died
suggested the importance of paucimannosidic/highly mannosidic N-glycan structures on fibrinogen
as factors that are related to unwanted cardiovascular events and all-cause mortality and can possibly
be seen as a prediction tool. Altered glycan structures composed of mannose residues are expected
to affect the reactivity of mannosylated glycoproteins with mannose-binding lectin and possibly
the entire cascade of events linked to this lectin. Since patients with ESRD are prone to cardiovascular
complications and the formation of atherosclerotic plaques, one can hypothesize that fibrinogen
with increasingly exposed mannose residues may contribute to the unwanted events.
PB  - Switzerland : Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Cells
T1  - Prediction of Mortality in Patients on Peritoneal Dialysis Based on the Fibrinogen Mannosylation
VL  - 12
IS  - 351
DO  - 10.3390/cells12030351
ER  - 

@article{
author = "Baralić, Marko and Pažitná, Lucia and Brković, Voin and Laušević, Mirjana and Gligorijević, Nikola and Katrlík, Jaroslav and Nedić, Olgica and Robajac, Dragana",
year = "2023",
abstract = "As we already reported, fibrinogen fucosylation emerged as a prognostic marker of peritoneal
membrane function in end-stage renal disease (ESRD) patients on peritoneal dialysis. After a
follow-up period of 18 months, we estimated the ability of employed lectins, as well as other biochemical
parameters, to serve as mortality predictors in these patients. Following a univariate Cox
regression analysis, ferritin, urea clearance, residual diuresis, hyperglycemia, and an increase in the
signal intensity obtained with Galanthus nivalis lectin (GNL) emerged as potential mortality predictors,
but additional multivariate Cox regression analysis pointed only to glucose concentration
and GNL as mortality predictors. Higher signal intensity obtained with GNL in patients that died
suggested the importance of paucimannosidic/highly mannosidic N-glycan structures on fibrinogen
as factors that are related to unwanted cardiovascular events and all-cause mortality and can possibly
be seen as a prediction tool. Altered glycan structures composed of mannose residues are expected
to affect the reactivity of mannosylated glycoproteins with mannose-binding lectin and possibly
the entire cascade of events linked to this lectin. Since patients with ESRD are prone to cardiovascular
complications and the formation of atherosclerotic plaques, one can hypothesize that fibrinogen
with increasingly exposed mannose residues may contribute to the unwanted events.",
publisher = "Switzerland : Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Cells",
title = "Prediction of Mortality in Patients on Peritoneal Dialysis Based on the Fibrinogen Mannosylation",
volume = "12",
number = "351",
doi = "10.3390/cells12030351"
}

Baralić, M., Pažitná, L., Brković, V., Laušević, M., Gligorijević, N., Katrlík, J., Nedić, O.,& Robajac, D.. (2023). Prediction of Mortality in Patients on Peritoneal Dialysis Based on the Fibrinogen Mannosylation. in Cells
Switzerland : Multidisciplinary Digital Publishing Institute (MDPI)., 12(351).
https://doi.org/10.3390/cells12030351

Baralić M, Pažitná L, Brković V, Laušević M, Gligorijević N, Katrlík J, Nedić O, Robajac D. Prediction of Mortality in Patients on Peritoneal Dialysis Based on the Fibrinogen Mannosylation. in Cells. 2023;12(351).
doi:10.3390/cells12030351 .

Baralić, Marko, Pažitná, Lucia, Brković, Voin, Laušević, Mirjana, Gligorijević, Nikola, Katrlík, Jaroslav, Nedić, Olgica, Robajac, Dragana, "Prediction of Mortality in Patients on Peritoneal Dialysis Based on the Fibrinogen Mannosylation" in Cells, 12, no. 351 (2023),
https://doi.org/10.3390/cells12030351 . .

TY  - CONF
AU  - Gligorijević, Nikola
AU  - Šunderić, Miloš
AU  - Vilotić, Aleksandra
AU  - Baralić, Marko
AU  - Nedić, Olgica
PY  - 2019
UR  - http://www.bds.org.rs/download/SBS_Conference_09_2019.pdf
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7291
AB  - Alpha-2-macroglobulin (α2M) is a homotetrameric blood glycoprotein having molecular
mass of 720 kDa which acts as a general protease inhibitor 1. So far, the methods to
estimate the quantity of α2M and its activity were separate procedures. The quantity is
usually measured by immunochemical assays and the anti-protease activity of α2M by
measuring the activity of trypsin bound to α2M using chromogenic substrate BAPNA 2. A
simple and reliable method for determination of the concentration and function of α2M by
zymography was developed. This method is based on the covalent binding of α2M and
trypsin followed by non-reducing PAGE and zymography with gelatine incorporated in the
electrophoretic gel. The results have shown that α2M binds trypsin in a linear,
concentration-dependent manner. The sensitivity of the method is 125 nM with an intraassay
coefficient of variation 4.2 %. Freezing of α2M induces its partial denaturation,
which can be seen as the reduction in the amount of functional molecule and its reactivity
with trypsin. The method was further tested using α2M from patients with an end-stage
renal disease who are known to be under an increased oxidative stress and inflammation,
which are expected to modify the structure of proteins. Using α2M from these patients,
lower affinity of α2M towards trypsin was detected when compaired to α2M isolated from
healthy persons. The reported zymographic method enables measurement of α2M taking
into consideration both its quantity and function, stressing the importance of determination
of the amount of physiologically active molecules and not just their total amount present in
the sample. Monitoring of the relation quantity/activity becomes very important when the
sample originates from an individual exposed to a stress or with a disease accompanied by
post-translational modifications of proteins such as diabetes, renal disease or cancer 3.
Presented method also enables determination of α2M in the presence of different modifying
chemical substances.
PB  - Serbian Biochemical Society
C3  - Serbian Biochemical Society Ninth Conference with international participation,  “Diversity in Biochemistry”, 14-16.11.2019, University of Belgrade – Kolarac Endowment, Belgrade, Serbia
T1  - Quantitation of the active alpha-2-macroglobulin by trypsin protease zymography
SP  - 98
EP  - 98
UR  - https://hdl.handle.net/21.15107/rcub_cer_7291
ER  - 

@conference{
author = "Gligorijević, Nikola and Šunderić, Miloš and Vilotić, Aleksandra and Baralić, Marko and Nedić, Olgica",
year = "2019",
abstract = "Alpha-2-macroglobulin (α2M) is a homotetrameric blood glycoprotein having molecular
mass of 720 kDa which acts as a general protease inhibitor 1. So far, the methods to
estimate the quantity of α2M and its activity were separate procedures. The quantity is
usually measured by immunochemical assays and the anti-protease activity of α2M by
measuring the activity of trypsin bound to α2M using chromogenic substrate BAPNA 2. A
simple and reliable method for determination of the concentration and function of α2M by
zymography was developed. This method is based on the covalent binding of α2M and
trypsin followed by non-reducing PAGE and zymography with gelatine incorporated in the
electrophoretic gel. The results have shown that α2M binds trypsin in a linear,
concentration-dependent manner. The sensitivity of the method is 125 nM with an intraassay
coefficient of variation 4.2 %. Freezing of α2M induces its partial denaturation,
which can be seen as the reduction in the amount of functional molecule and its reactivity
with trypsin. The method was further tested using α2M from patients with an end-stage
renal disease who are known to be under an increased oxidative stress and inflammation,
which are expected to modify the structure of proteins. Using α2M from these patients,
lower affinity of α2M towards trypsin was detected when compaired to α2M isolated from
healthy persons. The reported zymographic method enables measurement of α2M taking
into consideration both its quantity and function, stressing the importance of determination
of the amount of physiologically active molecules and not just their total amount present in
the sample. Monitoring of the relation quantity/activity becomes very important when the
sample originates from an individual exposed to a stress or with a disease accompanied by
post-translational modifications of proteins such as diabetes, renal disease or cancer 3.
Presented method also enables determination of α2M in the presence of different modifying
chemical substances.",
publisher = "Serbian Biochemical Society",
journal = "Serbian Biochemical Society Ninth Conference with international participation,  “Diversity in Biochemistry”, 14-16.11.2019, University of Belgrade – Kolarac Endowment, Belgrade, Serbia",
title = "Quantitation of the active alpha-2-macroglobulin by trypsin protease zymography",
pages = "98-98",
url = "https://hdl.handle.net/21.15107/rcub_cer_7291"
}

Gligorijević, N., Šunderić, M., Vilotić, A., Baralić, M.,& Nedić, O.. (2019). Quantitation of the active alpha-2-macroglobulin by trypsin protease zymography. in Serbian Biochemical Society Ninth Conference with international participation,  “Diversity in Biochemistry”, 14-16.11.2019, University of Belgrade – Kolarac Endowment, Belgrade, Serbia
Serbian Biochemical Society., 98-98.
https://hdl.handle.net/21.15107/rcub_cer_7291

Gligorijević N, Šunderić M, Vilotić A, Baralić M, Nedić O. Quantitation of the active alpha-2-macroglobulin by trypsin protease zymography. in Serbian Biochemical Society Ninth Conference with international participation,  “Diversity in Biochemistry”, 14-16.11.2019, University of Belgrade – Kolarac Endowment, Belgrade, Serbia. 2019;:98-98.
https://hdl.handle.net/21.15107/rcub_cer_7291 .

Gligorijević, Nikola, Šunderić, Miloš, Vilotić, Aleksandra, Baralić, Marko, Nedić, Olgica, "Quantitation of the active alpha-2-macroglobulin by trypsin protease zymography" in Serbian Biochemical Society Ninth Conference with international participation,  “Diversity in Biochemistry”, 14-16.11.2019, University of Belgrade – Kolarac Endowment, Belgrade, Serbia (2019):98-98,
https://hdl.handle.net/21.15107/rcub_cer_7291 .