TY  - CONF
AU  - Stojanović, Srđan
AU  - Niketić, Vesna
AU  - Spasić, Mihajlo
PY  - 1999
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6579
AB  - Reakcije NO sa prelaznim metalima čine osnovu za dejsvo NO u biološkim sistemima.
Reakcije NO sa proteinima koji sadrže hem intenzivno su izučavane. Medjutim, reakcije NO sa drugim metalnim centrima koji se nalaze u biološkim sistemima mnogo su manje ispitivane. Mi smo nedavno pokazali da pri tetiranju Mn i FeSOD sa NO nastaju reaktivni nitrozil kompleksi koji dismutuju NO dajući NO+ i NO’ jone.1 Pored toga, pronašli smo da se u cerebrospinalnoj tečnosti (CSF) nalaze supstance male molekulske mase koje takodje dismutuju NO. Pošto su nitrozo kompleksi gvoždja i aminokiselina opisani u literaturi, a pošto CSF sadrži visoke koncentracije gvoždja (ca. 0.8 pM) i raznih aminokiselina ( ca. 5 - 440 pM) u ovom radu smo ispitivali da li kompleksi gvoždja i aminokiselina imaju sposobnost da dismutuju NO. Da bismo to postigli, razne aminokiseline (ca 10 pM) i Fe2+ (0.8 pM) u 50 mM fosfatnom puferu pH 7.3 su izlagane NO pod anaerobnim uslovima do 6 sati. Dismutacija NO je utvrdjena na osnovu nastajanja značajnih količina nitrita, sporednog proizvoda reakcije NO+ i NO' sa vodom i NO.
Zaključeno je da nitrozo kompleksi gvoždja i aminokiselina vrše efikasnu dlsmutaclju NO: neki kompleksi su davali i do 50 pmol/pmol Fe2+/min nitrita. Efikasnost ovih kompleksa da dismutuju NO prevazilazi efikasnost FeSOD. Važno je primetiti da je ovo suprotno od ponašanja Cu2 + kompleksa aminokiselina koji dismutuju O2 ' , ali mnogo manje efikasno od Cu/ZnSOD. (Potencijalni) biohemijski značaj opisanih rezultata biće ukratko diskutovan.
PB  - Belgrade : Serbian Chemical Society
C3  - 6. jugoslovenski simpozijum biohemije, Program, Izvodi radova, 15-17 oktobar 1999, Beograd
T1  - Dismutacija azot oksida (NO) kompleksima gvožđa i aminokiselina
SP  - 52
EP  - 52
UR  - https://hdl.handle.net/21.15107/rcub_cer_6579
ER  - 

@conference{
author = "Stojanović, Srđan and Niketić, Vesna and Spasić, Mihajlo",
year = "1999",
abstract = "Reakcije NO sa prelaznim metalima čine osnovu za dejsvo NO u biološkim sistemima.
Reakcije NO sa proteinima koji sadrže hem intenzivno su izučavane. Medjutim, reakcije NO sa drugim metalnim centrima koji se nalaze u biološkim sistemima mnogo su manje ispitivane. Mi smo nedavno pokazali da pri tetiranju Mn i FeSOD sa NO nastaju reaktivni nitrozil kompleksi koji dismutuju NO dajući NO+ i NO’ jone.1 Pored toga, pronašli smo da se u cerebrospinalnoj tečnosti (CSF) nalaze supstance male molekulske mase koje takodje dismutuju NO. Pošto su nitrozo kompleksi gvoždja i aminokiselina opisani u literaturi, a pošto CSF sadrži visoke koncentracije gvoždja (ca. 0.8 pM) i raznih aminokiselina ( ca. 5 - 440 pM) u ovom radu smo ispitivali da li kompleksi gvoždja i aminokiselina imaju sposobnost da dismutuju NO. Da bismo to postigli, razne aminokiseline (ca 10 pM) i Fe2+ (0.8 pM) u 50 mM fosfatnom puferu pH 7.3 su izlagane NO pod anaerobnim uslovima do 6 sati. Dismutacija NO je utvrdjena na osnovu nastajanja značajnih količina nitrita, sporednog proizvoda reakcije NO+ i NO' sa vodom i NO.
Zaključeno je da nitrozo kompleksi gvoždja i aminokiselina vrše efikasnu dlsmutaclju NO: neki kompleksi su davali i do 50 pmol/pmol Fe2+/min nitrita. Efikasnost ovih kompleksa da dismutuju NO prevazilazi efikasnost FeSOD. Važno je primetiti da je ovo suprotno od ponašanja Cu2 + kompleksa aminokiselina koji dismutuju O2 ' , ali mnogo manje efikasno od Cu/ZnSOD. (Potencijalni) biohemijski značaj opisanih rezultata biće ukratko diskutovan.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "6. jugoslovenski simpozijum biohemije, Program, Izvodi radova, 15-17 oktobar 1999, Beograd",
title = "Dismutacija azot oksida (NO) kompleksima gvožđa i aminokiselina",
pages = "52-52",
url = "https://hdl.handle.net/21.15107/rcub_cer_6579"
}

Stojanović, S., Niketić, V.,& Spasić, M.. (1999). Dismutacija azot oksida (NO) kompleksima gvožđa i aminokiselina. in 6. jugoslovenski simpozijum biohemije, Program, Izvodi radova, 15-17 oktobar 1999, Beograd
Belgrade : Serbian Chemical Society., 52-52.
https://hdl.handle.net/21.15107/rcub_cer_6579

Stojanović S, Niketić V, Spasić M. Dismutacija azot oksida (NO) kompleksima gvožđa i aminokiselina. in 6. jugoslovenski simpozijum biohemije, Program, Izvodi radova, 15-17 oktobar 1999, Beograd. 1999;:52-52.
https://hdl.handle.net/21.15107/rcub_cer_6579 .

Stojanović, Srđan, Niketić, Vesna, Spasić, Mihajlo, "Dismutacija azot oksida (NO) kompleksima gvožđa i aminokiselina" in 6. jugoslovenski simpozijum biohemije, Program, Izvodi radova, 15-17 oktobar 1999, Beograd (1999):52-52,
https://hdl.handle.net/21.15107/rcub_cer_6579 .

TY  - CONF
AU  - Marinković, Zorica
AU  - Nikolić, Aleksandra
AU  - Stojanović, Srđan
AU  - Blagojević, Duško
AU  - Niketić, Vesna
AU  - Radunović, A,
AU  - Apostolski, S.
AU  - Spasić, Mihajlo
PY  - 1997
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6540
AB  - The aim of this study was to investigate the content of -SU groups and SOD activity m cerebrospinal fluid (CSF) both in ALS patients and control group, as well as to perform an in vitro lest for CSF NO binding capacity, in same examines. Nitric oxide for in vitro testing was provided by adding 40% sodium nitrite solution in the solution of 10% ferrous sulfate in 50% sulfuric acid. Our results showed significant increase -SH content in CSF of ALS patients in comparison with control group (114 ± 29 nmol -SH/mg proteins in CSF of ALS patients compared with 37 ± 12 nmol - SI Lmg proteins in CSF of controls)(p<0.05). After in vitro saturation of CSF with NO, we noted (he significant decrease of -SH content which was not found in control group. We also found that SOD activity in CSF of ALS patients was higher than in control group (16.1 ±2.8 U7 mg CSF proteins of ALS patients compared to 10.2 ± 2 U/ mg CSF proteins of control group). These results may confirm an increased ROS production in ALS patients without increased NO production.
C3  - 8th International Symposium on ALS/MND, Glasgow
T1  - Content of NO reactive -SH and Superoxide dismutase activity in CSF of ALS patients
UR  - https://hdl.handle.net/21.15107/rcub_cer_6540
ER  - 

@conference{
author = "Marinković, Zorica and Nikolić, Aleksandra and Stojanović, Srđan and Blagojević, Duško and Niketić, Vesna and Radunović, A, and Apostolski, S. and Spasić, Mihajlo",
year = "1997",
abstract = "The aim of this study was to investigate the content of -SU groups and SOD activity m cerebrospinal fluid (CSF) both in ALS patients and control group, as well as to perform an in vitro lest for CSF NO binding capacity, in same examines. Nitric oxide for in vitro testing was provided by adding 40% sodium nitrite solution in the solution of 10% ferrous sulfate in 50% sulfuric acid. Our results showed significant increase -SH content in CSF of ALS patients in comparison with control group (114 ± 29 nmol -SH/mg proteins in CSF of ALS patients compared with 37 ± 12 nmol - SI Lmg proteins in CSF of controls)(p<0.05). After in vitro saturation of CSF with NO, we noted (he significant decrease of -SH content which was not found in control group. We also found that SOD activity in CSF of ALS patients was higher than in control group (16.1 ±2.8 U7 mg CSF proteins of ALS patients compared to 10.2 ± 2 U/ mg CSF proteins of control group). These results may confirm an increased ROS production in ALS patients without increased NO production.",
journal = "8th International Symposium on ALS/MND, Glasgow",
title = "Content of NO reactive -SH and Superoxide dismutase activity in CSF of ALS patients",
url = "https://hdl.handle.net/21.15107/rcub_cer_6540"
}

Marinković, Z., Nikolić, A., Stojanović, S., Blagojević, D., Niketić, V., Radunović, A., Apostolski, S.,& Spasić, M.. (1997). Content of NO reactive -SH and Superoxide dismutase activity in CSF of ALS patients. in 8th International Symposium on ALS/MND, Glasgow.
https://hdl.handle.net/21.15107/rcub_cer_6540

Marinković Z, Nikolić A, Stojanović S, Blagojević D, Niketić V, Radunović A, Apostolski S, Spasić M. Content of NO reactive -SH and Superoxide dismutase activity in CSF of ALS patients. in 8th International Symposium on ALS/MND, Glasgow. 1997;.
https://hdl.handle.net/21.15107/rcub_cer_6540 .

Marinković, Zorica, Nikolić, Aleksandra, Stojanović, Srđan, Blagojević, Duško, Niketić, Vesna, Radunović, A,, Apostolski, S., Spasić, Mihajlo, "Content of NO reactive -SH and Superoxide dismutase activity in CSF of ALS patients" in 8th International Symposium on ALS/MND, Glasgow (1997),
https://hdl.handle.net/21.15107/rcub_cer_6540 .