TY  - JOUR
AU  - Trišić, Dijana
AU  - Ćetenović, Bojana
AU  - Zdravković, Nemanja
AU  - Marković, Tatjana
AU  - Dojčinović, Biljana
AU  - Jokanović, Vukoman
AU  - Marković, Dejan
PY  - 2019
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3231
AB  - Background/Aim. The main task of endodontic treatment is to eliminate pathologically altered tissue, to disinfect root canal space and to obtain its three-dimensional hermetic obturation. The main purpose of this study was to evaluate antimicrobial activity of new endodontic nano-structured highly active calcium silicates based materials albo-mineral plyoxide carbonate aggregate (ALBO-MPCA) and calcium silicates (CS) in comparison to mineral trioxide aggregate (MTA+) and UltraCal XS (CH). Methods. The antimicrobial activity of materials was tested against Staphylococcus aureus (ATCC 25923) and Enterococcus faecalis (ATCC 14506) strains, and following clinical isolates: Rothia dentocariosa, Enterococcus faecalis, Staphylococcus aureus, Streptococcus anginosus and Streptococcus vestibularis using a double layer agar diffusion test. The pH measurements were performed using the pH meter. Total amount of released ions was determined by inductively coupled plasma optical emission spectrometry (ICP-OES). Results. All tested materials showed the best antibacterial potential after 1 h of incubation. After 3h and 24h of the incubation period, the antibacterial potential of all tested materials were similar. The Agar diffusion test showed that ALBO-MPCA, CS and MTA+ had similar inhibition zones (p > 0.05), except in the activity against Staphylococcus aureus where ALBO-MPCA showed better antimicrobial properties than MTA+ in 3h and 24h of the incubation period (p < 0.05). Following 24h of the incubation, the inhibition zones were the strongest with CH against Staphylococcus aureus (16.67 ± 2.34 mm) followed by ALBO-MPCA (14.67 ± 1.21 mm) and the weakest with CS against Enterococcus faecalis (6.50 ± 1.76 mm). CH showed the highest pH, followed by ALBO-MPCA, CS and MTA+. Conclusion. The expressed antibacterial effects indicate that materials based on nano-structured highly active calcium silicates represent effective therapeutic agents for root canal obturation in one-visit apexification treatment, therefore they are recommend for further examination and clinical trials as they are proposed for MTA substitution.
PB  - Military Medical Academy, INI
T2  - Vojnosanitetski pregled
T1  - Antibacterial effects of new endodontic materials based on calcium silicates
T1  - Antibakterijski efekti novih endodontskih materijala na bazi kalcijum silikata
VL  - 76
IS  - 4
SP  - 365
EP  - 372
DO  - 10.2298/VSP161231130T
ER  - 

@article{
author = "Trišić, Dijana and Ćetenović, Bojana and Zdravković, Nemanja and Marković, Tatjana and Dojčinović, Biljana and Jokanović, Vukoman and Marković, Dejan",
year = "2019",
abstract = "Background/Aim. The main task of endodontic treatment is to eliminate pathologically altered tissue, to disinfect root canal space and to obtain its three-dimensional hermetic obturation. The main purpose of this study was to evaluate antimicrobial activity of new endodontic nano-structured highly active calcium silicates based materials albo-mineral plyoxide carbonate aggregate (ALBO-MPCA) and calcium silicates (CS) in comparison to mineral trioxide aggregate (MTA+) and UltraCal XS (CH). Methods. The antimicrobial activity of materials was tested against Staphylococcus aureus (ATCC 25923) and Enterococcus faecalis (ATCC 14506) strains, and following clinical isolates: Rothia dentocariosa, Enterococcus faecalis, Staphylococcus aureus, Streptococcus anginosus and Streptococcus vestibularis using a double layer agar diffusion test. The pH measurements were performed using the pH meter. Total amount of released ions was determined by inductively coupled plasma optical emission spectrometry (ICP-OES). Results. All tested materials showed the best antibacterial potential after 1 h of incubation. After 3h and 24h of the incubation period, the antibacterial potential of all tested materials were similar. The Agar diffusion test showed that ALBO-MPCA, CS and MTA+ had similar inhibition zones (p > 0.05), except in the activity against Staphylococcus aureus where ALBO-MPCA showed better antimicrobial properties than MTA+ in 3h and 24h of the incubation period (p < 0.05). Following 24h of the incubation, the inhibition zones were the strongest with CH against Staphylococcus aureus (16.67 ± 2.34 mm) followed by ALBO-MPCA (14.67 ± 1.21 mm) and the weakest with CS against Enterococcus faecalis (6.50 ± 1.76 mm). CH showed the highest pH, followed by ALBO-MPCA, CS and MTA+. Conclusion. The expressed antibacterial effects indicate that materials based on nano-structured highly active calcium silicates represent effective therapeutic agents for root canal obturation in one-visit apexification treatment, therefore they are recommend for further examination and clinical trials as they are proposed for MTA substitution.",
publisher = "Military Medical Academy, INI",
journal = "Vojnosanitetski pregled",
title = "Antibacterial effects of new endodontic materials based on calcium silicates, Antibakterijski efekti novih endodontskih materijala na bazi kalcijum silikata",
volume = "76",
number = "4",
pages = "365-372",
doi = "10.2298/VSP161231130T"
}

Trišić, D., Ćetenović, B., Zdravković, N., Marković, T., Dojčinović, B., Jokanović, V.,& Marković, D.. (2019). Antibacterial effects of new endodontic materials based on calcium silicates. in Vojnosanitetski pregled
Military Medical Academy, INI., 76(4), 365-372.
https://doi.org/10.2298/VSP161231130T

Trišić D, Ćetenović B, Zdravković N, Marković T, Dojčinović B, Jokanović V, Marković D. Antibacterial effects of new endodontic materials based on calcium silicates. in Vojnosanitetski pregled. 2019;76(4):365-372.
doi:10.2298/VSP161231130T .

Trišić, Dijana, Ćetenović, Bojana, Zdravković, Nemanja, Marković, Tatjana, Dojčinović, Biljana, Jokanović, Vukoman, Marković, Dejan, "Antibacterial effects of new endodontic materials based on calcium silicates" in Vojnosanitetski pregled, 76, no. 4 (2019):365-372,
https://doi.org/10.2298/VSP161231130T . .

TY  - JOUR
AU  - Cetenovic, Bojana
AU  - Prokic, Bogomir
AU  - Vasilijic, Sasa
AU  - Dojčinović, Biljana
AU  - Magic, Marko
AU  - Jokanovic, Vukoman
AU  - Marković, Dejan
PY  - 2017
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2186
AB  - Introduction: The aim of this article was to analyze biocompatibility and bioactivity of new endodontic materials on the basis of nanosynthesized calcium silicates (ALBO-MPCA(1) and ALBO-MPCA(2)) combined with different radiopacifiers in comparison with MTA(+). Methods: Morphology of the samples was studied by scanning electron microscopy, and the pH and ion release analysis were also assessed. Biocompatibility of materials' eluates (24-hour, 7-day, and 21-day) was conducted by using MIT test. Twelve New Zealand white rabbits were used for intraosseous implantation. Four calvarial defects per animal were created and filled with freshly prepared investigated materials. Results: Samples mostly consisted of agglomerates built up from nanoparticles, preferably spherical and rod-like. There was no significant difference among pH values of materials' eluates after 24 hours (P>.05). The amount of calcium and aluminum ion release decreased, whereas the amount of magnesium and bismuth (ALBO-MPCAl, MTA(+)) and barium (ALBO-MPCA(2)) increased during 21-day period. The metabolic activity of cells increased after the extraction time, except in case of undiluted elutes of ALBO-MPCA(2) and ALBO-MPCAI (21-day). Histologic analysis of the samples revealed newly formed bone tissue with moderate inflammation for all investigated materials, which subsided during 90-day period to mild. Both MIA(+) and ALBO-MPCAI were in direct contact with the newly formed bone tissue. After 90 days, statistically significant difference in hard tissue formation was observed in comparison of MIA(+) and ALBO-MPCki with control group (P  LT .05). Conclusions: Experimental materials ALBOMPCA, and ALBO-MPCA(2) possess both biocompatibility and bioactivity. Because ALBO-MPCAA provokes favorable biological response, it is especially good candidate for further clinical investigations.
PB  - Elsevier Science Inc, New York
T2  - Journal of Endodontics
T1  - Biocompatibility Investigation of New Endodontic Materials Based on Nanosynthesized Calcium Silicates Combined with Different Radiopacifiers
VL  - 43
IS  - 3
SP  - 425
EP  - 432
DO  - 10.1016/j.joen.2016.10.041
ER  - 

@article{
author = "Cetenovic, Bojana and Prokic, Bogomir and Vasilijic, Sasa and Dojčinović, Biljana and Magic, Marko and Jokanovic, Vukoman and Marković, Dejan",
year = "2017",
abstract = "Introduction: The aim of this article was to analyze biocompatibility and bioactivity of new endodontic materials on the basis of nanosynthesized calcium silicates (ALBO-MPCA(1) and ALBO-MPCA(2)) combined with different radiopacifiers in comparison with MTA(+). Methods: Morphology of the samples was studied by scanning electron microscopy, and the pH and ion release analysis were also assessed. Biocompatibility of materials' eluates (24-hour, 7-day, and 21-day) was conducted by using MIT test. Twelve New Zealand white rabbits were used for intraosseous implantation. Four calvarial defects per animal were created and filled with freshly prepared investigated materials. Results: Samples mostly consisted of agglomerates built up from nanoparticles, preferably spherical and rod-like. There was no significant difference among pH values of materials' eluates after 24 hours (P>.05). The amount of calcium and aluminum ion release decreased, whereas the amount of magnesium and bismuth (ALBO-MPCAl, MTA(+)) and barium (ALBO-MPCA(2)) increased during 21-day period. The metabolic activity of cells increased after the extraction time, except in case of undiluted elutes of ALBO-MPCA(2) and ALBO-MPCAI (21-day). Histologic analysis of the samples revealed newly formed bone tissue with moderate inflammation for all investigated materials, which subsided during 90-day period to mild. Both MIA(+) and ALBO-MPCAI were in direct contact with the newly formed bone tissue. After 90 days, statistically significant difference in hard tissue formation was observed in comparison of MIA(+) and ALBO-MPCki with control group (P  LT .05). Conclusions: Experimental materials ALBOMPCA, and ALBO-MPCA(2) possess both biocompatibility and bioactivity. Because ALBO-MPCAA provokes favorable biological response, it is especially good candidate for further clinical investigations.",
publisher = "Elsevier Science Inc, New York",
journal = "Journal of Endodontics",
title = "Biocompatibility Investigation of New Endodontic Materials Based on Nanosynthesized Calcium Silicates Combined with Different Radiopacifiers",
volume = "43",
number = "3",
pages = "425-432",
doi = "10.1016/j.joen.2016.10.041"
}

Cetenovic, B., Prokic, B., Vasilijic, S., Dojčinović, B., Magic, M., Jokanovic, V.,& Marković, D.. (2017). Biocompatibility Investigation of New Endodontic Materials Based on Nanosynthesized Calcium Silicates Combined with Different Radiopacifiers. in Journal of Endodontics
Elsevier Science Inc, New York., 43(3), 425-432.
https://doi.org/10.1016/j.joen.2016.10.041

Cetenovic B, Prokic B, Vasilijic S, Dojčinović B, Magic M, Jokanovic V, Marković D. Biocompatibility Investigation of New Endodontic Materials Based on Nanosynthesized Calcium Silicates Combined with Different Radiopacifiers. in Journal of Endodontics. 2017;43(3):425-432.
doi:10.1016/j.joen.2016.10.041 .

Cetenovic, Bojana, Prokic, Bogomir, Vasilijic, Sasa, Dojčinović, Biljana, Magic, Marko, Jokanovic, Vukoman, Marković, Dejan, "Biocompatibility Investigation of New Endodontic Materials Based on Nanosynthesized Calcium Silicates Combined with Different Radiopacifiers" in Journal of Endodontics, 43, no. 3 (2017):425-432,
https://doi.org/10.1016/j.joen.2016.10.041 . .

TY  - JOUR
AU  - Perić, Tamara O.
AU  - Marković, Dejan
AU  - Radojević, Vesna
AU  - Jančić-Heinemann, Radmila
AU  - Petrovic, Bojan B
AU  - Lamovec, Jelena
PY  - 2014
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1469
AB  - Purpose: The purpose of the study was to evaluate the surface characteristics of demineralized enamel after treatment with pastes containing casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) or casein phosphopeptide-amorphous calcium fluoride phosphate (CPP-ACFP) and to compare their efficacy with that of 0.05% NaF. Methods: Following formation of the artificial carious lesion, enamel slabs were divided into 4 groups (CPP-ACP, CPP-ACFP, 0.05% NaF and control) and submitted to a chemical caries model. Remineralization potential was examined using scanning electron microscope, energy-dispersive spectroscopy (EDS) and microhardness test. Scanning electron microphotographs were analyzed for area, minimal, maximal and mean diameter, perimeter, roundness and number of enamel defects and percentage of tooth surface affected by defects. Results: Treatment with 0.05% NaF partly reduced the appearance of enamel defects when compared with irregular demineralized enamel. Treatment with CPP-ACP or CPP-ACFP resulted in occlusion of defects which produced more flattened enamel surface. Image analysis revealed reduction of the dimensions of the defects in the 3 experimental groups. Treatment with CPP-ACFP decreased the number of enamel defects when compared with demineralized enamel. The EDS analysis did not show differences in Ca/O, P/O and Ca/P ratios between the groups (P>0.05). Microhardness test revealed significant effects of CPP-ACP and CPP-ACFP (P LT 0.05). Conclusions: Pastes containing CPP-ACP or CPP-ACFP showed potential to remineralize enamel surface lesions.
PB  - Wichtig Editore, Milan
T2  - Journal of Applied Biomaterials & Functional Materials
T1  - Influence of pastes containing casein phosphopeptide-amorphous calcium phosphate on surface of demineralized enamel
VL  - 12
IS  - 3
SP  - 234
EP  - 239
DO  - 10.5301/jabfm.5000194
ER  - 

@article{
author = "Perić, Tamara O. and Marković, Dejan and Radojević, Vesna and Jančić-Heinemann, Radmila and Petrovic, Bojan B and Lamovec, Jelena",
year = "2014",
abstract = "Purpose: The purpose of the study was to evaluate the surface characteristics of demineralized enamel after treatment with pastes containing casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) or casein phosphopeptide-amorphous calcium fluoride phosphate (CPP-ACFP) and to compare their efficacy with that of 0.05% NaF. Methods: Following formation of the artificial carious lesion, enamel slabs were divided into 4 groups (CPP-ACP, CPP-ACFP, 0.05% NaF and control) and submitted to a chemical caries model. Remineralization potential was examined using scanning electron microscope, energy-dispersive spectroscopy (EDS) and microhardness test. Scanning electron microphotographs were analyzed for area, minimal, maximal and mean diameter, perimeter, roundness and number of enamel defects and percentage of tooth surface affected by defects. Results: Treatment with 0.05% NaF partly reduced the appearance of enamel defects when compared with irregular demineralized enamel. Treatment with CPP-ACP or CPP-ACFP resulted in occlusion of defects which produced more flattened enamel surface. Image analysis revealed reduction of the dimensions of the defects in the 3 experimental groups. Treatment with CPP-ACFP decreased the number of enamel defects when compared with demineralized enamel. The EDS analysis did not show differences in Ca/O, P/O and Ca/P ratios between the groups (P>0.05). Microhardness test revealed significant effects of CPP-ACP and CPP-ACFP (P LT 0.05). Conclusions: Pastes containing CPP-ACP or CPP-ACFP showed potential to remineralize enamel surface lesions.",
publisher = "Wichtig Editore, Milan",
journal = "Journal of Applied Biomaterials & Functional Materials",
title = "Influence of pastes containing casein phosphopeptide-amorphous calcium phosphate on surface of demineralized enamel",
volume = "12",
number = "3",
pages = "234-239",
doi = "10.5301/jabfm.5000194"
}

Perić, T. O., Marković, D., Radojević, V., Jančić-Heinemann, R., Petrovic, B. B.,& Lamovec, J.. (2014). Influence of pastes containing casein phosphopeptide-amorphous calcium phosphate on surface of demineralized enamel. in Journal of Applied Biomaterials & Functional Materials
Wichtig Editore, Milan., 12(3), 234-239.
https://doi.org/10.5301/jabfm.5000194

Perić TO, Marković D, Radojević V, Jančić-Heinemann R, Petrovic BB, Lamovec J. Influence of pastes containing casein phosphopeptide-amorphous calcium phosphate on surface of demineralized enamel. in Journal of Applied Biomaterials & Functional Materials. 2014;12(3):234-239.
doi:10.5301/jabfm.5000194 .

Perić, Tamara O., Marković, Dejan, Radojević, Vesna, Jančić-Heinemann, Radmila, Petrovic, Bojan B, Lamovec, Jelena, "Influence of pastes containing casein phosphopeptide-amorphous calcium phosphate on surface of demineralized enamel" in Journal of Applied Biomaterials & Functional Materials, 12, no. 3 (2014):234-239,
https://doi.org/10.5301/jabfm.5000194 . .