TY  - JOUR
AU  - Stanković, Dalibor
AU  - Samphao, Anchalee
AU  - Dojčinović, Biljana
AU  - Kalcher, Kurt
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1997
AB  - This work presents the electrochemical behavior of levodopa (L-DOPA), at boron-doped diamond (BDD) electrodes, using cycling voltammetry (CV), in Britton-Robinson (BR) buffer solution, and application of the proposed electrode for the determination of L-DOPA in extracts from the seeds of velvet bean (Mucuna prurita Hook or Mucuna pruriens (L.) DC.). L-DOPA provides a well-defined and single oval-shape oxidation peak at +0.8 V vs. Ag/AgCl (3 M KCl) reference electrode in BR buffer solution at pH 3.0. Experimental parameters, such as pH of supporting electrolyte and square wave voltammetry (SWV) operating parameters (frequency and modulation amplitude) were optimized. The effect of possible interferences was evaluated. Under optimal conditions the detection limit of the developed method was 0.8 mu M and the calibration curve for L-DOPA was linear in the range from 2 to 100 mu M. The proposed method was successfully applied to the determination of L-DOPA in an extract from the seeds of Mucuna prurita. The obtained result was in good agreement with obtained by photometry with 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS). The developed approach can be beneficial for the quantification of L-DOPA using a BDD electrode as up-to-date potential alternative sensor for electroanalytical applications.
PB  - Slovensko Kemijsko Drustvo, Ljubljana
T2  - Acta Chimica Slovenica
T1  - Rapid Electrochemical Method for the Determination of L-DOPA in Extract From the Seeds of Mucuna Prurita
VL  - 63
IS  - 2
SP  - 220
EP  - 226
DO  - 10.17344/acsi.2015.1541
ER  - 

@article{
author = "Stanković, Dalibor and Samphao, Anchalee and Dojčinović, Biljana and Kalcher, Kurt",
year = "2016",
abstract = "This work presents the electrochemical behavior of levodopa (L-DOPA), at boron-doped diamond (BDD) electrodes, using cycling voltammetry (CV), in Britton-Robinson (BR) buffer solution, and application of the proposed electrode for the determination of L-DOPA in extracts from the seeds of velvet bean (Mucuna prurita Hook or Mucuna pruriens (L.) DC.). L-DOPA provides a well-defined and single oval-shape oxidation peak at +0.8 V vs. Ag/AgCl (3 M KCl) reference electrode in BR buffer solution at pH 3.0. Experimental parameters, such as pH of supporting electrolyte and square wave voltammetry (SWV) operating parameters (frequency and modulation amplitude) were optimized. The effect of possible interferences was evaluated. Under optimal conditions the detection limit of the developed method was 0.8 mu M and the calibration curve for L-DOPA was linear in the range from 2 to 100 mu M. The proposed method was successfully applied to the determination of L-DOPA in an extract from the seeds of Mucuna prurita. The obtained result was in good agreement with obtained by photometry with 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS). The developed approach can be beneficial for the quantification of L-DOPA using a BDD electrode as up-to-date potential alternative sensor for electroanalytical applications.",
publisher = "Slovensko Kemijsko Drustvo, Ljubljana",
journal = "Acta Chimica Slovenica",
title = "Rapid Electrochemical Method for the Determination of L-DOPA in Extract From the Seeds of Mucuna Prurita",
volume = "63",
number = "2",
pages = "220-226",
doi = "10.17344/acsi.2015.1541"
}

Stanković, D., Samphao, A., Dojčinović, B.,& Kalcher, K.. (2016). Rapid Electrochemical Method for the Determination of L-DOPA in Extract From the Seeds of Mucuna Prurita. in Acta Chimica Slovenica
Slovensko Kemijsko Drustvo, Ljubljana., 63(2), 220-226.
https://doi.org/10.17344/acsi.2015.1541

Stanković D, Samphao A, Dojčinović B, Kalcher K. Rapid Electrochemical Method for the Determination of L-DOPA in Extract From the Seeds of Mucuna Prurita. in Acta Chimica Slovenica. 2016;63(2):220-226.
doi:10.17344/acsi.2015.1541 .

Stanković, Dalibor, Samphao, Anchalee, Dojčinović, Biljana, Kalcher, Kurt, "Rapid Electrochemical Method for the Determination of L-DOPA in Extract From the Seeds of Mucuna Prurita" in Acta Chimica Slovenica, 63, no. 2 (2016):220-226,
https://doi.org/10.17344/acsi.2015.1541 . .