TY  - JOUR
AU  - Knežević, Aleksandar
AU  - Đokić, Ivana
AU  - Tosti, Tomislav
AU  - Popović, Slađana
AU  - Milojković-Opsenica, Dušanka
AU  - Vukojević, Jelena
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7232
AB  - The aim of the study was the comparative analysis of degradation of wheat straw lignin by white-rot fungi and its  implications on the efficiency of enzymatic hydrolysis of holocellulose. Peroxidases were found to be predominantly  responsible for lignin degradation, even though high laccase activities were detected, especially in the initial stages of  fungal culturing. The studied fungal species showed various ability to degrade lignin in wheat straw, which further  affected the release of reducing sugars during enzymatic saccharification. The highest rate of lignin, hemicelluloses and  cellulose degradation was noticed in the sample pretreated with Irpex lacteus. Among all the tested species, only  Ganoderma resinaceum was found as a suitable lignin degrader, with 2-fold higher hydrolysis yield (51.1 ± 4.7%) than  in the control. A key mechanism that enhances convertibility of carbohydrates is the selective lignin removal from biomass. Operating time, holocellulose loss and unpredictable fungal response to culturing conditions are the main  challenges in fungal pretreatment of lignocellulosic feedstock.
PB  - Institutul de Chimie Macromoleculara Petru Poni
T2  - Cellulose Chemistry and Technology
T1  - White-rot fungal pretreatment of wheat straw: effect on enzymatic hydrolysis of carbohydrate polymers
VL  - 57
IS  - 7-8
SP  - 815
EP  - 828
DO  - 10.35812/CelluloseChemTechnol.2023.57.72
ER  - 

@article{
author = "Knežević, Aleksandar and Đokić, Ivana and Tosti, Tomislav and Popović, Slađana and Milojković-Opsenica, Dušanka and Vukojević, Jelena",
year = "2023",
abstract = "The aim of the study was the comparative analysis of degradation of wheat straw lignin by white-rot fungi and its  implications on the efficiency of enzymatic hydrolysis of holocellulose. Peroxidases were found to be predominantly  responsible for lignin degradation, even though high laccase activities were detected, especially in the initial stages of  fungal culturing. The studied fungal species showed various ability to degrade lignin in wheat straw, which further  affected the release of reducing sugars during enzymatic saccharification. The highest rate of lignin, hemicelluloses and  cellulose degradation was noticed in the sample pretreated with Irpex lacteus. Among all the tested species, only  Ganoderma resinaceum was found as a suitable lignin degrader, with 2-fold higher hydrolysis yield (51.1 ± 4.7%) than  in the control. A key mechanism that enhances convertibility of carbohydrates is the selective lignin removal from biomass. Operating time, holocellulose loss and unpredictable fungal response to culturing conditions are the main  challenges in fungal pretreatment of lignocellulosic feedstock.",
publisher = "Institutul de Chimie Macromoleculara Petru Poni",
journal = "Cellulose Chemistry and Technology",
title = "White-rot fungal pretreatment of wheat straw: effect on enzymatic hydrolysis of carbohydrate polymers",
volume = "57",
number = "7-8",
pages = "815-828",
doi = "10.35812/CelluloseChemTechnol.2023.57.72"
}

Knežević, A., Đokić, I., Tosti, T., Popović, S., Milojković-Opsenica, D.,& Vukojević, J.. (2023). White-rot fungal pretreatment of wheat straw: effect on enzymatic hydrolysis of carbohydrate polymers. in Cellulose Chemistry and Technology
Institutul de Chimie Macromoleculara Petru Poni., 57(7-8), 815-828.
https://doi.org/10.35812/CelluloseChemTechnol.2023.57.72

Knežević A, Đokić I, Tosti T, Popović S, Milojković-Opsenica D, Vukojević J. White-rot fungal pretreatment of wheat straw: effect on enzymatic hydrolysis of carbohydrate polymers. in Cellulose Chemistry and Technology. 2023;57(7-8):815-828.
doi:10.35812/CelluloseChemTechnol.2023.57.72 .

Knežević, Aleksandar, Đokić, Ivana, Tosti, Tomislav, Popović, Slađana, Milojković-Opsenica, Dušanka, Vukojević, Jelena, "White-rot fungal pretreatment of wheat straw: effect on enzymatic hydrolysis of carbohydrate polymers" in Cellulose Chemistry and Technology, 57, no. 7-8 (2023):815-828,
https://doi.org/10.35812/CelluloseChemTechnol.2023.57.72 . .

TY  - CONF
AU  - Simić, Aleksandar
AU  - Tosti, Tomislav
AU  - Dramićanin, Aleksandra M.
AU  - Šegan, Sandra
AU  - Milojković-Opsenica, Dušanka
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6735
AB  - Sadržaj limunske kiseline u medu može da pruži informacije za procenu autenticnosti
meda. Me utim, važeci Codexalimentarius kao i Pravilnik o kvalitetu meda ne pružaju
informacije o sadržaju limunske kiseline u medu. Imajuci to u vidu, predmet ovog rada je
razvoj i validacija metode za odre ivanje citrata u medu. Analiza citrata ura ena je
primenom jonske hromatografijesa konduktometrijskom detekcijom na DIONEX AS 15
analitickoj koloni uz upotrebu 30 mM kalijum-hidroksida kao mobilne faze.Validovana
metoda se može primeniti za odre ivanje sadržaja citrata u medu u intervalu 1 – 100 mg/kg
s agranicom detekcije od 0,36 mg/kg igranicomkvantifikacije 1,20 mg/kg.Tacnost metode
je proverena testom obogacenja (95 – 101%), a preciznost ponovljenim analizama u toku
više dana. Metoda je proverena analizom 25 uzoraka bagremovog meda pri cemu je
sadržaj citrata bio u opsegu 10,82 – 55,74 mg/kg. Parametri validacije metode potvr uju
mogucnost primene jonskehromatografije sa konduktometrijskom detekcijom za brzo i
tacno odre ivanje citrata u uzorcima bagremovog meda.
AB  - The citric acid content can give useful information about honey authenticity. However, Codex Alimentarius and the Serbian regulation do not provide information on the content of citric acid in honey. Hence,the object of this paper is the method development and validation for the determination of citrate content in honey. The concentration of citrate in honey was determined by ion chromatography with conductivity detection on DIONEX AS 15 column and 30 mM potassium-hydroxide as mobile phase. The method validation revealed that method can be used for determination of citrate in acaciahoney from 1 to100 mg/kg. The limit of detection was 0.36 mg/kg and limit of quantification 1.20 mg/kg. The accuracy was determined with recovery test (95–101%), and precision with repeat analysis during few days. Analysis of 25 acacia honey samples confirmed applicability of method and revealed that amount of citrate was10.82 – 55.74 mg/kg. The statistical parameters of method validation pointed out that the ion chromatography with conductometry detection enabled rapid and accurate determination of citrate in acacia honey.
PB  - Belgrade : Serbian Chemical Society
C3  - Kratki izvodi radova, knjiga radova - 58. Savetovanje Srpskog hemijskog društva, 9. i 10. jun 2022. godine, Beograd / Book of Abstracts, Proceedings - 58th Meeting of the Serbian Chemical Society, June 9-10, 2023, Belgrade, Serbia
T1  - Validacija metode za određivanje sadržaja citrata u bagremovom medu jonskom hromatografijom
T1  - Method validation for determining the citrate content in acacia honey by ion  chromatography
SP  - 56
EP  - 56
UR  - https://hdl.handle.net/21.15107/rcub_cer_6735
ER  - 

@conference{
author = "Simić, Aleksandar and Tosti, Tomislav and Dramićanin, Aleksandra M. and Šegan, Sandra and Milojković-Opsenica, Dušanka",
year = "2022",
abstract = "Sadržaj limunske kiseline u medu može da pruži informacije za procenu autenticnosti
meda. Me utim, važeci Codexalimentarius kao i Pravilnik o kvalitetu meda ne pružaju
informacije o sadržaju limunske kiseline u medu. Imajuci to u vidu, predmet ovog rada je
razvoj i validacija metode za odre ivanje citrata u medu. Analiza citrata ura ena je
primenom jonske hromatografijesa konduktometrijskom detekcijom na DIONEX AS 15
analitickoj koloni uz upotrebu 30 mM kalijum-hidroksida kao mobilne faze.Validovana
metoda se može primeniti za odre ivanje sadržaja citrata u medu u intervalu 1 – 100 mg/kg
s agranicom detekcije od 0,36 mg/kg igranicomkvantifikacije 1,20 mg/kg.Tacnost metode
je proverena testom obogacenja (95 – 101%), a preciznost ponovljenim analizama u toku
više dana. Metoda je proverena analizom 25 uzoraka bagremovog meda pri cemu je
sadržaj citrata bio u opsegu 10,82 – 55,74 mg/kg. Parametri validacije metode potvr uju
mogucnost primene jonskehromatografije sa konduktometrijskom detekcijom za brzo i
tacno odre ivanje citrata u uzorcima bagremovog meda., The citric acid content can give useful information about honey authenticity. However, Codex Alimentarius and the Serbian regulation do not provide information on the content of citric acid in honey. Hence,the object of this paper is the method development and validation for the determination of citrate content in honey. The concentration of citrate in honey was determined by ion chromatography with conductivity detection on DIONEX AS 15 column and 30 mM potassium-hydroxide as mobile phase. The method validation revealed that method can be used for determination of citrate in acaciahoney from 1 to100 mg/kg. The limit of detection was 0.36 mg/kg and limit of quantification 1.20 mg/kg. The accuracy was determined with recovery test (95–101%), and precision with repeat analysis during few days. Analysis of 25 acacia honey samples confirmed applicability of method and revealed that amount of citrate was10.82 – 55.74 mg/kg. The statistical parameters of method validation pointed out that the ion chromatography with conductometry detection enabled rapid and accurate determination of citrate in acacia honey.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "Kratki izvodi radova, knjiga radova - 58. Savetovanje Srpskog hemijskog društva, 9. i 10. jun 2022. godine, Beograd / Book of Abstracts, Proceedings - 58th Meeting of the Serbian Chemical Society, June 9-10, 2023, Belgrade, Serbia",
title = "Validacija metode za određivanje sadržaja citrata u bagremovom medu jonskom hromatografijom, Method validation for determining the citrate content in acacia honey by ion  chromatography",
pages = "56-56",
url = "https://hdl.handle.net/21.15107/rcub_cer_6735"
}

Simić, A., Tosti, T., Dramićanin, A. M., Šegan, S.,& Milojković-Opsenica, D.. (2022). Validacija metode za određivanje sadržaja citrata u bagremovom medu jonskom hromatografijom. in Kratki izvodi radova, knjiga radova - 58. Savetovanje Srpskog hemijskog društva, 9. i 10. jun 2022. godine, Beograd / Book of Abstracts, Proceedings - 58th Meeting of the Serbian Chemical Society, June 9-10, 2023, Belgrade, Serbia
Belgrade : Serbian Chemical Society., 56-56.
https://hdl.handle.net/21.15107/rcub_cer_6735

Simić A, Tosti T, Dramićanin AM, Šegan S, Milojković-Opsenica D. Validacija metode za određivanje sadržaja citrata u bagremovom medu jonskom hromatografijom. in Kratki izvodi radova, knjiga radova - 58. Savetovanje Srpskog hemijskog društva, 9. i 10. jun 2022. godine, Beograd / Book of Abstracts, Proceedings - 58th Meeting of the Serbian Chemical Society, June 9-10, 2023, Belgrade, Serbia. 2022;:56-56.
https://hdl.handle.net/21.15107/rcub_cer_6735 .

Simić, Aleksandar, Tosti, Tomislav, Dramićanin, Aleksandra M., Šegan, Sandra, Milojković-Opsenica, Dušanka, "Validacija metode za određivanje sadržaja citrata u bagremovom medu jonskom hromatografijom" in Kratki izvodi radova, knjiga radova - 58. Savetovanje Srpskog hemijskog društva, 9. i 10. jun 2022. godine, Beograd / Book of Abstracts, Proceedings - 58th Meeting of the Serbian Chemical Society, June 9-10, 2023, Belgrade, Serbia (2022):56-56,
https://hdl.handle.net/21.15107/rcub_cer_6735 .

TY  - JOUR
AU  - Šegan, Sandra
AU  - Jevtić, Ivana
AU  - Tosti, Tomislav
AU  - Penjišević, Jelena
AU  - Šukalović, Vladimir
AU  - Kostić Rajačić, Slađana
AU  - Milojković-Opsenica, Dušanka
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5413
AB  - Lipophilicity and ionization constant of sixteen fentanyl analogues were investigated by reversed-phase thin-layer chromatography (RP-TLC). Fourteen compounds have nitrogen containing groups at C-3 position of the piperidine ring and two compounds are 3-carbomethoxy derivatives of fentanyl. Among them, five are diastereomeric cis/trans couples and six novel trans diastereomers. In addition, the lipophilicity and ionization constant of fentanyl were also determined by the same method, as a reference. The physicochemical property, lipophilicity, expressed as retention indices RM0, b, and C0, as well as PC1, was determined and correlated with in silico values. Ionization constants were determined on the basis of the relationships between analyte's retention expressed as RF and mobile phase pH. Calculated structural descriptors together with the retention indices, were subjected to the principal component analysis – PCA and hierarchical cluster analysis – HCA, in order to provide basic insights into the similarities among the studied compounds. The blood – brain barrier (BBB) permeation was investigated in the function of experimentally obtained values of lipophilicity, polar surface area and molecular weight. In general, results of the present research corroborate well with previously determined antinociceptive activities of the investigated compounds, pointing out that besides the cis/trans isomerism, another set of important parameters should be taken into account when designing new derivatives of C-3 substituted fentanyl, especially lipophilicity, voluminosity and HBD/A character of the substituents. Accordingly, RP-TLC can be considered as a valuable asset in the ligand-based drug design.
PB  - Elsevier
T2  - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
T1  - Determination of lipophilicity and ionization of fentanyl and its 3‑substituted analogs by reversed-phase thin-layer chromatography
VL  - 1211
SP  - 123481
DO  - 10.1016/j.jchromb.2022.123481
ER  - 

@article{
author = "Šegan, Sandra and Jevtić, Ivana and Tosti, Tomislav and Penjišević, Jelena and Šukalović, Vladimir and Kostić Rajačić, Slađana and Milojković-Opsenica, Dušanka",
year = "2022",
abstract = "Lipophilicity and ionization constant of sixteen fentanyl analogues were investigated by reversed-phase thin-layer chromatography (RP-TLC). Fourteen compounds have nitrogen containing groups at C-3 position of the piperidine ring and two compounds are 3-carbomethoxy derivatives of fentanyl. Among them, five are diastereomeric cis/trans couples and six novel trans diastereomers. In addition, the lipophilicity and ionization constant of fentanyl were also determined by the same method, as a reference. The physicochemical property, lipophilicity, expressed as retention indices RM0, b, and C0, as well as PC1, was determined and correlated with in silico values. Ionization constants were determined on the basis of the relationships between analyte's retention expressed as RF and mobile phase pH. Calculated structural descriptors together with the retention indices, were subjected to the principal component analysis – PCA and hierarchical cluster analysis – HCA, in order to provide basic insights into the similarities among the studied compounds. The blood – brain barrier (BBB) permeation was investigated in the function of experimentally obtained values of lipophilicity, polar surface area and molecular weight. In general, results of the present research corroborate well with previously determined antinociceptive activities of the investigated compounds, pointing out that besides the cis/trans isomerism, another set of important parameters should be taken into account when designing new derivatives of C-3 substituted fentanyl, especially lipophilicity, voluminosity and HBD/A character of the substituents. Accordingly, RP-TLC can be considered as a valuable asset in the ligand-based drug design.",
publisher = "Elsevier",
journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",
title = "Determination of lipophilicity and ionization of fentanyl and its 3‑substituted analogs by reversed-phase thin-layer chromatography",
volume = "1211",
pages = "123481",
doi = "10.1016/j.jchromb.2022.123481"
}

Šegan, S., Jevtić, I., Tosti, T., Penjišević, J., Šukalović, V., Kostić Rajačić, S.,& Milojković-Opsenica, D.. (2022). Determination of lipophilicity and ionization of fentanyl and its 3‑substituted analogs by reversed-phase thin-layer chromatography. in Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Elsevier., 1211, 123481.
https://doi.org/10.1016/j.jchromb.2022.123481

Šegan S, Jevtić I, Tosti T, Penjišević J, Šukalović V, Kostić Rajačić S, Milojković-Opsenica D. Determination of lipophilicity and ionization of fentanyl and its 3‑substituted analogs by reversed-phase thin-layer chromatography. in Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2022;1211:123481.
doi:10.1016/j.jchromb.2022.123481 .

Šegan, Sandra, Jevtić, Ivana, Tosti, Tomislav, Penjišević, Jelena, Šukalović, Vladimir, Kostić Rajačić, Slađana, Milojković-Opsenica, Dušanka, "Determination of lipophilicity and ionization of fentanyl and its 3‑substituted analogs by reversed-phase thin-layer chromatography" in Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 1211 (2022):123481,
https://doi.org/10.1016/j.jchromb.2022.123481 . .

TY  - JOUR
AU  - Šegan, Sandra
AU  - Živković-Radovanović, Vukosava
AU  - Tosti, Tomislav
AU  - Ristivojević, Petar
AU  - Milojković-Opsenica, Dušanka
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4770
AB  - Growing antibiotic resistance creates a need to find new antimicrobial agents characterized by diverse chemical structures and pharmaceutical activity. The higher plants synthesize many specialized metabolites as a part of their normal metabolic activity and have been extensively used for centuries in treatment of different diseases. They have a wide activity range depending on the species, topography and climate, and may have different categories of active principles. In contrast to conventional antimicrobial techniques, planar chromatography in combination with biological detection can be an appropriate method of choice for fast, simple, and low-cost screening of plant extract for successful detection of antimicrobial agents which can be good candidates for lead compounds. To date, all bioautography steps such as chromatographic separation, detection with bacteria cells, incubation, and visualization of bioactive bands were improved and optimized. This review gives an overview of bioautography procedure from extraction to structure elucidation of antimicrobial compounds from plants.
PB  - USA :Taylor & Francis INC
T2  - Journal of Liquid Chromatography & Related Technologies
T1  - Thin-layer chromatography in bioassays of antimicrobial compounds from plants
DO  - 10.1080/10826076.2021.1968429
ER  - 

@article{
author = "Šegan, Sandra and Živković-Radovanović, Vukosava and Tosti, Tomislav and Ristivojević, Petar and Milojković-Opsenica, Dušanka",
year = "2021",
abstract = "Growing antibiotic resistance creates a need to find new antimicrobial agents characterized by diverse chemical structures and pharmaceutical activity. The higher plants synthesize many specialized metabolites as a part of their normal metabolic activity and have been extensively used for centuries in treatment of different diseases. They have a wide activity range depending on the species, topography and climate, and may have different categories of active principles. In contrast to conventional antimicrobial techniques, planar chromatography in combination with biological detection can be an appropriate method of choice for fast, simple, and low-cost screening of plant extract for successful detection of antimicrobial agents which can be good candidates for lead compounds. To date, all bioautography steps such as chromatographic separation, detection with bacteria cells, incubation, and visualization of bioactive bands were improved and optimized. This review gives an overview of bioautography procedure from extraction to structure elucidation of antimicrobial compounds from plants.",
publisher = "USA :Taylor & Francis INC",
journal = "Journal of Liquid Chromatography & Related Technologies",
title = "Thin-layer chromatography in bioassays of antimicrobial compounds from plants",
doi = "10.1080/10826076.2021.1968429"
}

Šegan, S., Živković-Radovanović, V., Tosti, T., Ristivojević, P.,& Milojković-Opsenica, D.. (2021). Thin-layer chromatography in bioassays of antimicrobial compounds from plants. in Journal of Liquid Chromatography & Related Technologies
USA :Taylor & Francis INC..
https://doi.org/10.1080/10826076.2021.1968429

Šegan S, Živković-Radovanović V, Tosti T, Ristivojević P, Milojković-Opsenica D. Thin-layer chromatography in bioassays of antimicrobial compounds from plants. in Journal of Liquid Chromatography & Related Technologies. 2021;.
doi:10.1080/10826076.2021.1968429 .

Šegan, Sandra, Živković-Radovanović, Vukosava, Tosti, Tomislav, Ristivojević, Petar, Milojković-Opsenica, Dušanka, "Thin-layer chromatography in bioassays of antimicrobial compounds from plants" in Journal of Liquid Chromatography & Related Technologies (2021),
https://doi.org/10.1080/10826076.2021.1968429 . .

TY  - CONF
AU  - Milojković-Opsenica, Dušanka
AU  - Rosić, Anđelka
AU  - Krstić, Đurđa
AU  - Trifković, Jelena
AU  - Tosti, Tomislav
AU  - Šegan, Sandra
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6736
AB  - Honey is a natural sweet product of honeybees widely consumed by people as both a food and
a medicine. It is mainly composed of carbohydrates, primarily fructose and glucose, followed by
sucrose and other oligosaccharides present in lower quantity. Due to its nutritional and health
benefits and relatively high price, honey is frequently subjected to adulteration either by
addition of sugar syrups or cheaper and lower quality honey, or by mislabeling of declaration.
Numerous analytical methods, such as chromatographic, spectroscopic, isotopic, and
electrochemical, have been developed to detect honey adulterants.[1] Application of thin-layer
chromatography (TLC) in the detection of syrup adulterants is limited due to the hydrolysis of
polysaccharides and a presence of false positive components such as smaller oligosaccharides
(from 3 to 6 monosaccharide units). However, with the development of high-performance layers
and sophisticated instrumentation, high-performance TLC (HPTLC) should become a method of
choice in determination of honey authenticity.
PB  - Croatian Chemical Society
C3  - Book of abstracts - 27th Croatian Meeting of Chemists and Chemical Engineers / 5 th Symposium Vladimir Prelog, 5-8 October 2021, Veli Lošinj, Vitality Hotel Punta, Croatia
T1  - Development and validation of HPTLC method for determination of sugar profiles of honey and its syrup adulterants
SP  - 244
EP  - 244
UR  - https://hdl.handle.net/21.15107/rcub_cer_6736
ER  - 

@conference{
author = "Milojković-Opsenica, Dušanka and Rosić, Anđelka and Krstić, Đurđa and Trifković, Jelena and Tosti, Tomislav and Šegan, Sandra",
year = "2021",
abstract = "Honey is a natural sweet product of honeybees widely consumed by people as both a food and
a medicine. It is mainly composed of carbohydrates, primarily fructose and glucose, followed by
sucrose and other oligosaccharides present in lower quantity. Due to its nutritional and health
benefits and relatively high price, honey is frequently subjected to adulteration either by
addition of sugar syrups or cheaper and lower quality honey, or by mislabeling of declaration.
Numerous analytical methods, such as chromatographic, spectroscopic, isotopic, and
electrochemical, have been developed to detect honey adulterants.[1] Application of thin-layer
chromatography (TLC) in the detection of syrup adulterants is limited due to the hydrolysis of
polysaccharides and a presence of false positive components such as smaller oligosaccharides
(from 3 to 6 monosaccharide units). However, with the development of high-performance layers
and sophisticated instrumentation, high-performance TLC (HPTLC) should become a method of
choice in determination of honey authenticity.",
publisher = "Croatian Chemical Society",
journal = "Book of abstracts - 27th Croatian Meeting of Chemists and Chemical Engineers / 5 th Symposium Vladimir Prelog, 5-8 October 2021, Veli Lošinj, Vitality Hotel Punta, Croatia",
title = "Development and validation of HPTLC method for determination of sugar profiles of honey and its syrup adulterants",
pages = "244-244",
url = "https://hdl.handle.net/21.15107/rcub_cer_6736"
}

Milojković-Opsenica, D., Rosić, A., Krstić, Đ., Trifković, J., Tosti, T.,& Šegan, S.. (2021). Development and validation of HPTLC method for determination of sugar profiles of honey and its syrup adulterants. in Book of abstracts - 27th Croatian Meeting of Chemists and Chemical Engineers / 5 th Symposium Vladimir Prelog, 5-8 October 2021, Veli Lošinj, Vitality Hotel Punta, Croatia
Croatian Chemical Society., 244-244.
https://hdl.handle.net/21.15107/rcub_cer_6736

Milojković-Opsenica D, Rosić A, Krstić Đ, Trifković J, Tosti T, Šegan S. Development and validation of HPTLC method for determination of sugar profiles of honey and its syrup adulterants. in Book of abstracts - 27th Croatian Meeting of Chemists and Chemical Engineers / 5 th Symposium Vladimir Prelog, 5-8 October 2021, Veli Lošinj, Vitality Hotel Punta, Croatia. 2021;:244-244.
https://hdl.handle.net/21.15107/rcub_cer_6736 .

Milojković-Opsenica, Dušanka, Rosić, Anđelka, Krstić, Đurđa, Trifković, Jelena, Tosti, Tomislav, Šegan, Sandra, "Development and validation of HPTLC method for determination of sugar profiles of honey and its syrup adulterants" in Book of abstracts - 27th Croatian Meeting of Chemists and Chemical Engineers / 5 th Symposium Vladimir Prelog, 5-8 October 2021, Veli Lošinj, Vitality Hotel Punta, Croatia (2021):244-244,
https://hdl.handle.net/21.15107/rcub_cer_6736 .

TY  - JOUR
AU  - Dramićanin, Aleksandra M.
AU  - Andrić, Filip
AU  - Mutić, Jelena
AU  - Stanković, Vesna
AU  - Momirović, Nebojša M.
AU  - Milojković-Opsenica, Dušanka
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4488
AB  - The effects of organic versus integral and conventional crop management on content and distribution of major and trace elements in different varieties of potato were investigated in a three year field trial. A set of 48 potato tubers of four varieties with different harvesting times, cultivated in three types of agricultural systems: conventional (C), integral (I), and organic (O) were characterized based on the composition of the elements of their bulk and peel. A total of 16 elements were quantified. In order to determine the source of variation among the types of production, parts of potato and varieties, multivariate analysis of variance (MANOVA) was conducted. The results indicate that Ca, Mg, and K may be considered as important indicators of the type of production, genotypes of potato, and harvesting time. Additionally, the analyses show that ten microelements are able to distinguish between production types and genotypes of potato.
PB  - Elsevier
T2  - Food Chemistry
T1  - Content and distribution of major and trace elements as a tool to assess the genotypes, harvesting time, and cultivation systems of potato
VL  - 354
SP  - 129507
DO  - 10.1016/j.foodchem.2021.129507
ER  - 

@article{
author = "Dramićanin, Aleksandra M. and Andrić, Filip and Mutić, Jelena and Stanković, Vesna and Momirović, Nebojša M. and Milojković-Opsenica, Dušanka",
year = "2021",
abstract = "The effects of organic versus integral and conventional crop management on content and distribution of major and trace elements in different varieties of potato were investigated in a three year field trial. A set of 48 potato tubers of four varieties with different harvesting times, cultivated in three types of agricultural systems: conventional (C), integral (I), and organic (O) were characterized based on the composition of the elements of their bulk and peel. A total of 16 elements were quantified. In order to determine the source of variation among the types of production, parts of potato and varieties, multivariate analysis of variance (MANOVA) was conducted. The results indicate that Ca, Mg, and K may be considered as important indicators of the type of production, genotypes of potato, and harvesting time. Additionally, the analyses show that ten microelements are able to distinguish between production types and genotypes of potato.",
publisher = "Elsevier",
journal = "Food Chemistry",
title = "Content and distribution of major and trace elements as a tool to assess the genotypes, harvesting time, and cultivation systems of potato",
volume = "354",
pages = "129507",
doi = "10.1016/j.foodchem.2021.129507"
}

Dramićanin, A. M., Andrić, F., Mutić, J., Stanković, V., Momirović, N. M.,& Milojković-Opsenica, D.. (2021). Content and distribution of major and trace elements as a tool to assess the genotypes, harvesting time, and cultivation systems of potato. in Food Chemistry
Elsevier., 354, 129507.
https://doi.org/10.1016/j.foodchem.2021.129507

Dramićanin AM, Andrić F, Mutić J, Stanković V, Momirović NM, Milojković-Opsenica D. Content and distribution of major and trace elements as a tool to assess the genotypes, harvesting time, and cultivation systems of potato. in Food Chemistry. 2021;354:129507.
doi:10.1016/j.foodchem.2021.129507 .

Dramićanin, Aleksandra M., Andrić, Filip, Mutić, Jelena, Stanković, Vesna, Momirović, Nebojša M., Milojković-Opsenica, Dušanka, "Content and distribution of major and trace elements as a tool to assess the genotypes, harvesting time, and cultivation systems of potato" in Food Chemistry, 354 (2021):129507,
https://doi.org/10.1016/j.foodchem.2021.129507 . .

TY  - JOUR
AU  - Šegan, Sandra
AU  - Penjišević, Jelena
AU  - Šukalović, Vladimir
AU  - Andrić, Deana
AU  - Milojković-Opsenica, Dušanka
AU  - Kostić Rajačić, Slađana
PY  - 2019
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2991
AB  - Reversed-phase thin-layer chromatography and micellar thin-layer chromatography were used in order to investigate retention behaviour and to determine lipophilicity of series of 2(methoxy)phenylpiperazine dopamine D2 ligands with different size, shape and rigidity. The retention mechanism was discussed. The lipophilicity parameters obtained in conventional reversed-phase systems expressed as RM0 and C0, as well as RM values
determined in microemulsion reversed-phase systems were correlated with in silico determined lipophilicity values. In silico pharmacokinetic properties of 2-(methoxy)phenylpiperazine dopamine D2 ligands revealed the importance of experimentally determined lipophilicity values besides the molecular weight, on the blood–brain barrier permeability process. Also, the experimentally determined lipophilicity was found as a very important factor in plasma protein binding process of 2-(methoxy)phenylpiperazine dopamine D2 ligands. Besides, the Lipinski's rule of five indicates that examined ligands satisfy the criterion of drug-like molecules. The principal component analysis was performed on the experimentally determined and calculated lipophilicity values as well on the molecular descriptors which describe the pharmacokinetic properties in order to provide basic insights into similarities among the studied ligands.
PB  - Elsevier
T2  - Journal of Chromatography B
T1  - Investigation of lipophilicity and pharmacokinetic properties of 2-(methoxy) phenylpiperazine dopamine D2 ligands
VL  - 1124
SP  - 146
EP  - 153
DO  - 10.1016/j.jchromb.2019.06.006
ER  - 

@article{
author = "Šegan, Sandra and Penjišević, Jelena and Šukalović, Vladimir and Andrić, Deana and Milojković-Opsenica, Dušanka and Kostić Rajačić, Slađana",
year = "2019",
abstract = "Reversed-phase thin-layer chromatography and micellar thin-layer chromatography were used in order to investigate retention behaviour and to determine lipophilicity of series of 2(methoxy)phenylpiperazine dopamine D2 ligands with different size, shape and rigidity. The retention mechanism was discussed. The lipophilicity parameters obtained in conventional reversed-phase systems expressed as RM0 and C0, as well as RM values
determined in microemulsion reversed-phase systems were correlated with in silico determined lipophilicity values. In silico pharmacokinetic properties of 2-(methoxy)phenylpiperazine dopamine D2 ligands revealed the importance of experimentally determined lipophilicity values besides the molecular weight, on the blood–brain barrier permeability process. Also, the experimentally determined lipophilicity was found as a very important factor in plasma protein binding process of 2-(methoxy)phenylpiperazine dopamine D2 ligands. Besides, the Lipinski's rule of five indicates that examined ligands satisfy the criterion of drug-like molecules. The principal component analysis was performed on the experimentally determined and calculated lipophilicity values as well on the molecular descriptors which describe the pharmacokinetic properties in order to provide basic insights into similarities among the studied ligands.",
publisher = "Elsevier",
journal = "Journal of Chromatography B",
title = "Investigation of lipophilicity and pharmacokinetic properties of 2-(methoxy) phenylpiperazine dopamine D2 ligands",
volume = "1124",
pages = "146-153",
doi = "10.1016/j.jchromb.2019.06.006"
}

Šegan, S., Penjišević, J., Šukalović, V., Andrić, D., Milojković-Opsenica, D.,& Kostić Rajačić, S.. (2019). Investigation of lipophilicity and pharmacokinetic properties of 2-(methoxy) phenylpiperazine dopamine D2 ligands. in Journal of Chromatography B
Elsevier., 1124, 146-153.
https://doi.org/10.1016/j.jchromb.2019.06.006

Šegan S, Penjišević J, Šukalović V, Andrić D, Milojković-Opsenica D, Kostić Rajačić S. Investigation of lipophilicity and pharmacokinetic properties of 2-(methoxy) phenylpiperazine dopamine D2 ligands. in Journal of Chromatography B. 2019;1124:146-153.
doi:10.1016/j.jchromb.2019.06.006 .

Šegan, Sandra, Penjišević, Jelena, Šukalović, Vladimir, Andrić, Deana, Milojković-Opsenica, Dušanka, Kostić Rajačić, Slađana, "Investigation of lipophilicity and pharmacokinetic properties of 2-(methoxy) phenylpiperazine dopamine D2 ligands" in Journal of Chromatography B, 1124 (2019):146-153,
https://doi.org/10.1016/j.jchromb.2019.06.006 . .

TY  - JOUR
AU  - Šegan, Sandra
AU  - Opsenica, Dejan
AU  - Milojković-Opsenica, Dušanka
PY  - 2019
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3205
AB  - Among widely used chromatographic methods modern thin-layer chromatography is not only the
simplest to perform but is also considered as respectable analytical method in various phases of
drug discovery and development processes such as monitoring of synthesis, identification of bioactive
substances from various natural sources and their isolation and purification, determination
of lipophilicity and other physico-chemical parameters, quantitative structure-activity relationship
studies, bioautography, as well as qualitative and quantitative analysis of drugs and their metabolites. An overview of recently published papers dealing with application of thin-layer chromatography in medicinal chemistry is presented.
PB  - Taylor & Francis Inc.
T2  - Journal of Liquid Chromatography & Related Technologies
T1  - Thin-layer chromatography in medicinal chemistry
VL  - 42
IS  - 9-10
SP  - 238
EP  - 248
DO  - 10.1080/10826076.2019.1585615
ER  - 

@article{
author = "Šegan, Sandra and Opsenica, Dejan and Milojković-Opsenica, Dušanka",
year = "2019",
abstract = "Among widely used chromatographic methods modern thin-layer chromatography is not only the
simplest to perform but is also considered as respectable analytical method in various phases of
drug discovery and development processes such as monitoring of synthesis, identification of bioactive
substances from various natural sources and their isolation and purification, determination
of lipophilicity and other physico-chemical parameters, quantitative structure-activity relationship
studies, bioautography, as well as qualitative and quantitative analysis of drugs and their metabolites. An overview of recently published papers dealing with application of thin-layer chromatography in medicinal chemistry is presented.",
publisher = "Taylor & Francis Inc.",
journal = "Journal of Liquid Chromatography & Related Technologies",
title = "Thin-layer chromatography in medicinal chemistry",
volume = "42",
number = "9-10",
pages = "238-248",
doi = "10.1080/10826076.2019.1585615"
}

Šegan, S., Opsenica, D.,& Milojković-Opsenica, D.. (2019). Thin-layer chromatography in medicinal chemistry. in Journal of Liquid Chromatography & Related Technologies
Taylor & Francis Inc.., 42(9-10), 238-248.
https://doi.org/10.1080/10826076.2019.1585615

Šegan S, Opsenica D, Milojković-Opsenica D. Thin-layer chromatography in medicinal chemistry. in Journal of Liquid Chromatography & Related Technologies. 2019;42(9-10):238-248.
doi:10.1080/10826076.2019.1585615 .

Šegan, Sandra, Opsenica, Dejan, Milojković-Opsenica, Dušanka, "Thin-layer chromatography in medicinal chemistry" in Journal of Liquid Chromatography & Related Technologies, 42, no. 9-10 (2019):238-248,
https://doi.org/10.1080/10826076.2019.1585615 . .

TY  - JOUR
AU  - Aleksić, Ivana
AU  - Ristivojević, Petar
AU  - Pavić, Aleksandar
AU  - Radojević, Ivana
AU  - Čomić, Ljiljana R.
AU  - Vasiljević, Branka
AU  - Opsenica, Dejan
AU  - Milojković-Opsenica, Dušanka
AU  - Šenerović, Lidija
PY  - 2018
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/2932
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3139
AB  - Ethnopharmacological relevance: Trapa natans L. (water chestnut or water caltrop) is a widespread aquatic plant, which has been cultivated for food and traditional medicine since ancient times. Pharmacological studies showed that water chestnut exhibits the wide range of biological activities, such as antimicrobial, antioxidative, analgesic, anti-inflammatory, as well as antiulcer. Aim of the study: Evaluation of anti-virulence potential and toxicity of T. natans methanol (TnM), acetone (TnA) and ethyl acetate (TnEA) leaf extracts. Materials and methods: The anti-quorum sensing activity of Tn extracts was addressed by measuring their effects on biofilm formation, swarming motility and pyocyanin and elastase production in Pseudomonas aeruginosa. Specific P. aeruginosa biosensors were used to identify which of the signaling pathways were affected. The lethal and developmental toxicity of extracts were addressed in vivo using the zebrafish (Danio rerio) model system. The phenolic composition of T. natans leafs extracts was analyzed by a linear ion trap-OrbiTrap hybrid mass spectrometer (LTQ OrbiTrapMS) and UHPLC system configured with a diode array detector (DAD) hyphenated with the triple quadrupole mass spectrometer. Results: Subinhibitory concentrations of Tn leaf extracts (0.2 MIC) inhibited pyocyanin and elastase production up to 50% and 60%, respectively, and reduced swarming zones, comparing to non-treated P. aeruginosa. TnA inhibited biofilm formation by 15%, TnM showed a stimulatory effect on biofilm formation up to 20%, while TnEA showed no effect. The bioactive concentrations of TnM and TnA were not toxic in the zebrafish model system. Twenty-two phenolic compounds were tentatively identified in TnM, where thirteen of them were identified in T. natans for the first time. Tn extracts, as well as their major components, ellagic and ferulic acids, demonstrated the ability to interfere with P. aeruginosa Las and PQS signaling pathways. Conclusions: This study demonstrates anti-virulence potential of Tn leaf extracts against medically important pathogen P. aeruginosa and confirms the ethnopharmacological application of this plant against microbial infections.
PB  - Elsevier Ireland Ltd, Clare
T2  - Journal of Ethnopharmacology
T1  - Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts
VL  - 222
SP  - 148
EP  - 158
DO  - 10.1016/j.jep.2018.05.005
ER  - 

@article{
author = "Aleksić, Ivana and Ristivojević, Petar and Pavić, Aleksandar and Radojević, Ivana and Čomić, Ljiljana R. and Vasiljević, Branka and Opsenica, Dejan and Milojković-Opsenica, Dušanka and Šenerović, Lidija",
year = "2018",
abstract = "Ethnopharmacological relevance: Trapa natans L. (water chestnut or water caltrop) is a widespread aquatic plant, which has been cultivated for food and traditional medicine since ancient times. Pharmacological studies showed that water chestnut exhibits the wide range of biological activities, such as antimicrobial, antioxidative, analgesic, anti-inflammatory, as well as antiulcer. Aim of the study: Evaluation of anti-virulence potential and toxicity of T. natans methanol (TnM), acetone (TnA) and ethyl acetate (TnEA) leaf extracts. Materials and methods: The anti-quorum sensing activity of Tn extracts was addressed by measuring their effects on biofilm formation, swarming motility and pyocyanin and elastase production in Pseudomonas aeruginosa. Specific P. aeruginosa biosensors were used to identify which of the signaling pathways were affected. The lethal and developmental toxicity of extracts were addressed in vivo using the zebrafish (Danio rerio) model system. The phenolic composition of T. natans leafs extracts was analyzed by a linear ion trap-OrbiTrap hybrid mass spectrometer (LTQ OrbiTrapMS) and UHPLC system configured with a diode array detector (DAD) hyphenated with the triple quadrupole mass spectrometer. Results: Subinhibitory concentrations of Tn leaf extracts (0.2 MIC) inhibited pyocyanin and elastase production up to 50% and 60%, respectively, and reduced swarming zones, comparing to non-treated P. aeruginosa. TnA inhibited biofilm formation by 15%, TnM showed a stimulatory effect on biofilm formation up to 20%, while TnEA showed no effect. The bioactive concentrations of TnM and TnA were not toxic in the zebrafish model system. Twenty-two phenolic compounds were tentatively identified in TnM, where thirteen of them were identified in T. natans for the first time. Tn extracts, as well as their major components, ellagic and ferulic acids, demonstrated the ability to interfere with P. aeruginosa Las and PQS signaling pathways. Conclusions: This study demonstrates anti-virulence potential of Tn leaf extracts against medically important pathogen P. aeruginosa and confirms the ethnopharmacological application of this plant against microbial infections.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Journal of Ethnopharmacology",
title = "Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts",
volume = "222",
pages = "148-158",
doi = "10.1016/j.jep.2018.05.005"
}

Aleksić, I., Ristivojević, P., Pavić, A., Radojević, I., Čomić, L. R., Vasiljević, B., Opsenica, D., Milojković-Opsenica, D.,& Šenerović, L.. (2018). Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts. in Journal of Ethnopharmacology
Elsevier Ireland Ltd, Clare., 222, 148-158.
https://doi.org/10.1016/j.jep.2018.05.005

Aleksić I, Ristivojević P, Pavić A, Radojević I, Čomić LR, Vasiljević B, Opsenica D, Milojković-Opsenica D, Šenerović L. Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts. in Journal of Ethnopharmacology. 2018;222:148-158.
doi:10.1016/j.jep.2018.05.005 .

Aleksić, Ivana, Ristivojević, Petar, Pavić, Aleksandar, Radojević, Ivana, Čomić, Ljiljana R., Vasiljević, Branka, Opsenica, Dejan, Milojković-Opsenica, Dušanka, Šenerović, Lidija, "Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts" in Journal of Ethnopharmacology, 222 (2018):148-158,
https://doi.org/10.1016/j.jep.2018.05.005 . .

TY  - CONF
AU  - Dragičević, Vesna
AU  - Opsenica, Dejan
AU  - Mesarović, Jelena
AU  - Brankov, Milan
AU  - Simić, Milena
AU  - Kravić, Natalija
AU  - Milojković-Opsenica, Dušanka
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7097
AB  - Germination is one of the most sensitive phases in growth of agricultural plants, where optimal conditions in temperature and water accessibility are required. Seedlings are particularly sensitive to environmental stressors, especially when maize lines are considered. They tend to germinate and grow slower, have poorer adaptability to stress and seeds could lose germination ability relative fast. Application of natural or synthetic substances could increases maize vigor, particularly of seeds with poor viability. It is proven that some stimulators positively affect growth, water absorption and antioxidative response of plants [1-4]. The aim of experiment was to examine the influence of four mixed tetraoxanes on germination and early growth (seven-day old seedlings) of maize inbred line with low germination ability (<50%).
PB  - Society of Physical Chemists of Serbia
C3  - Proceedings - Physical Chemistry 2018 - 14th International Conference on Fundamental and Applied Aspects of Physical Chemistry, September 24-28, 2018, Belgrade
T1  - The effect of some mixed tetraoxanes on germination and antioxidants in maize seedlings
VL  - 1
SP  - 547
EP  - 550
UR  - https://hdl.handle.net/21.15107/rcub_cer_7097
ER  - 

@conference{
author = "Dragičević, Vesna and Opsenica, Dejan and Mesarović, Jelena and Brankov, Milan and Simić, Milena and Kravić, Natalija and Milojković-Opsenica, Dušanka",
year = "2018",
abstract = "Germination is one of the most sensitive phases in growth of agricultural plants, where optimal conditions in temperature and water accessibility are required. Seedlings are particularly sensitive to environmental stressors, especially when maize lines are considered. They tend to germinate and grow slower, have poorer adaptability to stress and seeds could lose germination ability relative fast. Application of natural or synthetic substances could increases maize vigor, particularly of seeds with poor viability. It is proven that some stimulators positively affect growth, water absorption and antioxidative response of plants [1-4]. The aim of experiment was to examine the influence of four mixed tetraoxanes on germination and early growth (seven-day old seedlings) of maize inbred line with low germination ability (<50%).",
publisher = "Society of Physical Chemists of Serbia",
journal = "Proceedings - Physical Chemistry 2018 - 14th International Conference on Fundamental and Applied Aspects of Physical Chemistry, September 24-28, 2018, Belgrade",
title = "The effect of some mixed tetraoxanes on germination and antioxidants in maize seedlings",
volume = "1",
pages = "547-550",
url = "https://hdl.handle.net/21.15107/rcub_cer_7097"
}

Dragičević, V., Opsenica, D., Mesarović, J., Brankov, M., Simić, M., Kravić, N.,& Milojković-Opsenica, D.. (2018). The effect of some mixed tetraoxanes on germination and antioxidants in maize seedlings. in Proceedings - Physical Chemistry 2018 - 14th International Conference on Fundamental and Applied Aspects of Physical Chemistry, September 24-28, 2018, Belgrade
Society of Physical Chemists of Serbia., 1, 547-550.
https://hdl.handle.net/21.15107/rcub_cer_7097

Dragičević V, Opsenica D, Mesarović J, Brankov M, Simić M, Kravić N, Milojković-Opsenica D. The effect of some mixed tetraoxanes on germination and antioxidants in maize seedlings. in Proceedings - Physical Chemistry 2018 - 14th International Conference on Fundamental and Applied Aspects of Physical Chemistry, September 24-28, 2018, Belgrade. 2018;1:547-550.
https://hdl.handle.net/21.15107/rcub_cer_7097 .

Dragičević, Vesna, Opsenica, Dejan, Mesarović, Jelena, Brankov, Milan, Simić, Milena, Kravić, Natalija, Milojković-Opsenica, Dušanka, "The effect of some mixed tetraoxanes on germination and antioxidants in maize seedlings" in Proceedings - Physical Chemistry 2018 - 14th International Conference on Fundamental and Applied Aspects of Physical Chemistry, September 24-28, 2018, Belgrade, 1 (2018):547-550,
https://hdl.handle.net/21.15107/rcub_cer_7097 .

TY  - JOUR
AU  - Milojković-Opsenica, Dušanka
AU  - Andrić, Filip
AU  - Šegan, Sandra
AU  - Trifković, Jelena
AU  - Tešić, Živoslav
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2475
AB  - The methods of correlating molecular structure of substances expressed as descriptors, to their biological activity are commonly denoted as Quantitative Structure-Activity Relationships (QSARs). This concept, typically applied in drug discovery processes, is also widely used for correlation of molecular structure and physicochemical properties of solutes in so-called quantitative structure-property relationship (QSPR) studies, as well as for explanation of chromatographic behavior, i.e., separation mechanisms of analytes, where is termed as quantitative structure-retention relationship (QSRR). Mathematical expressions of structural characteristics of substances, named as molecular descriptors, can be calculated by various computational techniques or experimentally determined by different analytical methods. Thin-layer chromatography as a rapid, sensitive, and economical liquid chromatographic method has been widely used for determination of various chromatographic descriptors applicable in QSAR/QSPR studies. An overview of recently published papers dealing with this concept is presented. [GRAPHICS] .
PB  - Taylor & Francis Inc, Philadelphia
T2  - Journal of Liquid Chromatography & Related Technologies
T1  - Thin-layer chromatography in quantitative structure-activity relationship studies
VL  - 41
IS  - 6
SP  - 272
EP  - 281
DO  - 10.1080/10826076.2018.1447892
ER  - 

@article{
author = "Milojković-Opsenica, Dušanka and Andrić, Filip and Šegan, Sandra and Trifković, Jelena and Tešić, Živoslav",
year = "2018",
abstract = "The methods of correlating molecular structure of substances expressed as descriptors, to their biological activity are commonly denoted as Quantitative Structure-Activity Relationships (QSARs). This concept, typically applied in drug discovery processes, is also widely used for correlation of molecular structure and physicochemical properties of solutes in so-called quantitative structure-property relationship (QSPR) studies, as well as for explanation of chromatographic behavior, i.e., separation mechanisms of analytes, where is termed as quantitative structure-retention relationship (QSRR). Mathematical expressions of structural characteristics of substances, named as molecular descriptors, can be calculated by various computational techniques or experimentally determined by different analytical methods. Thin-layer chromatography as a rapid, sensitive, and economical liquid chromatographic method has been widely used for determination of various chromatographic descriptors applicable in QSAR/QSPR studies. An overview of recently published papers dealing with this concept is presented. [GRAPHICS] .",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Journal of Liquid Chromatography & Related Technologies",
title = "Thin-layer chromatography in quantitative structure-activity relationship studies",
volume = "41",
number = "6",
pages = "272-281",
doi = "10.1080/10826076.2018.1447892"
}

Milojković-Opsenica, D., Andrić, F., Šegan, S., Trifković, J.,& Tešić, Ž.. (2018). Thin-layer chromatography in quantitative structure-activity relationship studies. in Journal of Liquid Chromatography & Related Technologies
Taylor & Francis Inc, Philadelphia., 41(6), 272-281.
https://doi.org/10.1080/10826076.2018.1447892

Milojković-Opsenica D, Andrić F, Šegan S, Trifković J, Tešić Ž. Thin-layer chromatography in quantitative structure-activity relationship studies. in Journal of Liquid Chromatography & Related Technologies. 2018;41(6):272-281.
doi:10.1080/10826076.2018.1447892 .

Milojković-Opsenica, Dušanka, Andrić, Filip, Šegan, Sandra, Trifković, Jelena, Tešić, Živoslav, "Thin-layer chromatography in quantitative structure-activity relationship studies" in Journal of Liquid Chromatography & Related Technologies, 41, no. 6 (2018):272-281,
https://doi.org/10.1080/10826076.2018.1447892 . .

TY  - JOUR
AU  - Aleksić, Ivana
AU  - Ristivojevic, Petar
AU  - Pavić, Aleksandar
AU  - Radojevic, Ivana
AU  - Čomić, Ljiljana R.
AU  - Vasiljevic, Branka
AU  - Opsenica, Dejan
AU  - Milojković-Opsenica, Dušanka
AU  - Senerovic, Lidija
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2310
AB  - Ethnopharmacological relevance: Trapa natans L. (water chestnut or water caltrop) is a widespread aquatic plant, which has been cultivated for food and traditional medicine since ancient times. Pharmacological studies showed that water chestnut exhibits the wide range of biological activities, such as antimicrobial, antioxidative, analgesic, anti-inflammatory, as well as antiulcer. Aim of the study: Evaluation of anti-virulence potential and toxicity of T. natans methanol (TnM), acetone (TnA) and ethyl acetate (TnEA) leaf extracts. Materials and methods: The anti-quorum sensing activity of Tn extracts was addressed by measuring their effects on biofilm formation, swarming motility and pyocyanin and elastase production in Pseudomonas aeruginosa. Specific P. aeruginosa biosensors were used to identify which of the signaling pathways were affected. The lethal and developmental toxicity of extracts were addressed in vivo using the zebrafish (Danio rerio) model system. The phenolic composition of T. natans leafs extracts was analyzed by a linear ion trap-OrbiTrap hybrid mass spectrometer (LTQ OrbiTrapMS) and UHPLC system configured with a diode array detector (DAD) hyphenated with the triple quadrupole mass spectrometer. Results: Subinhibitory concentrations of Tn leaf extracts (0.2 MIC) inhibited pyocyanin and elastase production up to 50% and 60%, respectively, and reduced swarming zones, comparing to non-treated P. aeruginosa. TnA inhibited biofilm formation by 15%, TnM showed a stimulatory effect on biofilm formation up to 20%, while TnEA showed no effect. The bioactive concentrations of TnM and TnA were not toxic in the zebrafish model system. Twenty-two phenolic compounds were tentatively identified in TnM, where thirteen of them were identified in T. natans for the first time. Tn extracts, as well as their major components, ellagic and ferulic acids, demonstrated the ability to interfere with P. aeruginosa Las and PQS signaling pathways. Conclusions: This study demonstrates anti-virulence potential of Tn leaf extracts against medically important pathogen P. aeruginosa and confirms the ethnopharmacological application of this plant against microbial infections.
PB  - Elsevier Ireland Ltd, Clare
T2  - Journal of Ethnopharmacology
T1  - Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts
VL  - 222
SP  - 148
EP  - 158
DO  - 10.1016/j.jep.2018.05.005
ER  - 

@article{
author = "Aleksić, Ivana and Ristivojevic, Petar and Pavić, Aleksandar and Radojevic, Ivana and Čomić, Ljiljana R. and Vasiljevic, Branka and Opsenica, Dejan and Milojković-Opsenica, Dušanka and Senerovic, Lidija",
year = "2018",
abstract = "Ethnopharmacological relevance: Trapa natans L. (water chestnut or water caltrop) is a widespread aquatic plant, which has been cultivated for food and traditional medicine since ancient times. Pharmacological studies showed that water chestnut exhibits the wide range of biological activities, such as antimicrobial, antioxidative, analgesic, anti-inflammatory, as well as antiulcer. Aim of the study: Evaluation of anti-virulence potential and toxicity of T. natans methanol (TnM), acetone (TnA) and ethyl acetate (TnEA) leaf extracts. Materials and methods: The anti-quorum sensing activity of Tn extracts was addressed by measuring their effects on biofilm formation, swarming motility and pyocyanin and elastase production in Pseudomonas aeruginosa. Specific P. aeruginosa biosensors were used to identify which of the signaling pathways were affected. The lethal and developmental toxicity of extracts were addressed in vivo using the zebrafish (Danio rerio) model system. The phenolic composition of T. natans leafs extracts was analyzed by a linear ion trap-OrbiTrap hybrid mass spectrometer (LTQ OrbiTrapMS) and UHPLC system configured with a diode array detector (DAD) hyphenated with the triple quadrupole mass spectrometer. Results: Subinhibitory concentrations of Tn leaf extracts (0.2 MIC) inhibited pyocyanin and elastase production up to 50% and 60%, respectively, and reduced swarming zones, comparing to non-treated P. aeruginosa. TnA inhibited biofilm formation by 15%, TnM showed a stimulatory effect on biofilm formation up to 20%, while TnEA showed no effect. The bioactive concentrations of TnM and TnA were not toxic in the zebrafish model system. Twenty-two phenolic compounds were tentatively identified in TnM, where thirteen of them were identified in T. natans for the first time. Tn extracts, as well as their major components, ellagic and ferulic acids, demonstrated the ability to interfere with P. aeruginosa Las and PQS signaling pathways. Conclusions: This study demonstrates anti-virulence potential of Tn leaf extracts against medically important pathogen P. aeruginosa and confirms the ethnopharmacological application of this plant against microbial infections.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Journal of Ethnopharmacology",
title = "Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts",
volume = "222",
pages = "148-158",
doi = "10.1016/j.jep.2018.05.005"
}

Aleksić, I., Ristivojevic, P., Pavić, A., Radojevic, I., Čomić, L. R., Vasiljevic, B., Opsenica, D., Milojković-Opsenica, D.,& Senerovic, L.. (2018). Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts. in Journal of Ethnopharmacology
Elsevier Ireland Ltd, Clare., 222, 148-158.
https://doi.org/10.1016/j.jep.2018.05.005

Aleksić I, Ristivojevic P, Pavić A, Radojevic I, Čomić LR, Vasiljevic B, Opsenica D, Milojković-Opsenica D, Senerovic L. Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts. in Journal of Ethnopharmacology. 2018;222:148-158.
doi:10.1016/j.jep.2018.05.005 .

Aleksić, Ivana, Ristivojevic, Petar, Pavić, Aleksandar, Radojevic, Ivana, Čomić, Ljiljana R., Vasiljevic, Branka, Opsenica, Dejan, Milojković-Opsenica, Dušanka, Senerovic, Lidija, "Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts" in Journal of Ethnopharmacology, 222 (2018):148-158,
https://doi.org/10.1016/j.jep.2018.05.005 . .

TY  - JOUR
AU  - Šegan, Sandra
AU  - Opsenica, Igor
AU  - Zlatović, Mario
AU  - Milojković-Opsenica, Dušanka
AU  - Šolaja, Bogdan
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1960
AB  - The chromatographic behaviour of series of 4-amino-7-chloroquinoline (4,7-ACQ) based compounds was studied by reversed-phase thin-layer chromatography (RPTLC) with binary mobile phases containing water and the organic modifiers, DMSO or acetone. The lipophilicity of the studied compounds was determined by extrapolation of retention parameters R-M to pure water content in mobile phase. In order to obtain some basic insight into the chromatographic behaviour and structural features of investigated compounds, PCA was performed on both chromatographic data (R-M values) and calculated 2D and 3D structural descriptors. Both QSRR and QSAR models were built by means of the partial least squares (PLS) statistical method. It was found that descriptors which encode hydrophobic (dispersive) interactions have positive influence on retention, while influence of descriptors encoding polar interactions was negative. According to the obtained PLS model for inhibition of botulinum neurotoxin serotype A light chain, hydrophobic interactions influence positively on the mechanism of action of the investigated 4,7-ACQ while polar interactions are less favoured. Contrary, the results of PLS modelling of activity against Plasmodium falciparum strains (W2, D6 and TM91C235) indicate that higher polarity of 4,7-ACQ contribute to their higher antimalarial activity.
PB  - Elsevier
T2  - Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
T1  - Quantitative structure retention/activity relationships of biologically relevant 4-amino-7-chloroquinoline based compounds
VL  - 1012
SP  - 144
EP  - 152
DO  - 10.1016/j.jchromb.2016.01.033
ER  - 

@article{
author = "Šegan, Sandra and Opsenica, Igor and Zlatović, Mario and Milojković-Opsenica, Dušanka and Šolaja, Bogdan",
year = "2016",
abstract = "The chromatographic behaviour of series of 4-amino-7-chloroquinoline (4,7-ACQ) based compounds was studied by reversed-phase thin-layer chromatography (RPTLC) with binary mobile phases containing water and the organic modifiers, DMSO or acetone. The lipophilicity of the studied compounds was determined by extrapolation of retention parameters R-M to pure water content in mobile phase. In order to obtain some basic insight into the chromatographic behaviour and structural features of investigated compounds, PCA was performed on both chromatographic data (R-M values) and calculated 2D and 3D structural descriptors. Both QSRR and QSAR models were built by means of the partial least squares (PLS) statistical method. It was found that descriptors which encode hydrophobic (dispersive) interactions have positive influence on retention, while influence of descriptors encoding polar interactions was negative. According to the obtained PLS model for inhibition of botulinum neurotoxin serotype A light chain, hydrophobic interactions influence positively on the mechanism of action of the investigated 4,7-ACQ while polar interactions are less favoured. Contrary, the results of PLS modelling of activity against Plasmodium falciparum strains (W2, D6 and TM91C235) indicate that higher polarity of 4,7-ACQ contribute to their higher antimalarial activity.",
publisher = "Elsevier",
journal = "Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences",
title = "Quantitative structure retention/activity relationships of biologically relevant 4-amino-7-chloroquinoline based compounds",
volume = "1012",
pages = "144-152",
doi = "10.1016/j.jchromb.2016.01.033"
}

Šegan, S., Opsenica, I., Zlatović, M., Milojković-Opsenica, D.,& Šolaja, B.. (2016). Quantitative structure retention/activity relationships of biologically relevant 4-amino-7-chloroquinoline based compounds. in Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
Elsevier., 1012, 144-152.
https://doi.org/10.1016/j.jchromb.2016.01.033

Šegan S, Opsenica I, Zlatović M, Milojković-Opsenica D, Šolaja B. Quantitative structure retention/activity relationships of biologically relevant 4-amino-7-chloroquinoline based compounds. in Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences. 2016;1012:144-152.
doi:10.1016/j.jchromb.2016.01.033 .

Šegan, Sandra, Opsenica, Igor, Zlatović, Mario, Milojković-Opsenica, Dušanka, Šolaja, Bogdan, "Quantitative structure retention/activity relationships of biologically relevant 4-amino-7-chloroquinoline based compounds" in Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 1012 (2016):144-152,
https://doi.org/10.1016/j.jchromb.2016.01.033 . .

TY  - JOUR
AU  - Vukojević, Vesna
AU  - Trifković, Jelena
AU  - Krgovic, Rada
AU  - Milojković-Opsenica, Dušanka
AU  - Marković, Marijana
AU  - Amaizah, Naser Ramdan R.
AU  - Mutić, Jelena
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1913
AB  - Fourteen metals and metalloids were determined in Conyza canadensis L. harvested from the fly ash landfill of the thermoelectric power plant "Kolubara" (Serbia). Fly ash samples were collected together with the plant samples and subjected to sequential extraction according to the three-step sequential extraction scheme proposed by the Community Bureau of Reference (BCR; now the Standards, Measurements and Testing Program). The contents of metals and metalloids were determined by inductively coupled plasma optical emission spectrometry (ICP-OES) in plant root and the aboveground part and correlated with their contents in the fly ash samples. The bioconcentration factor (BCF) and translocation factors (TF) were calculated to access uptake of metals from fly ash and their translocation to the aboveground part. Results regarding As revealed that fly ash samples in the proximity of the active cassette had higher amounts of the element. Principal component analysis (PCA) showed that As had no impact on the classification of plant parts. BCF for As ranged from 1.44 to 23.8 and varied, depending on the investigated area; TF for As ranged from 0.43 to 2.61, indicating that the plant translocated As from root to shoot. In addition to As, Conyza canadensis L. exhibited efficient uptake of other metals from fly ash. According to the calculated BCF and TF, the plant retained Al, Fe and Cr in the root and translocated Zn, Cd, Cu and As from root to shoot in the course of the detoxifying process.
PB  - Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd
T2  - Archives of Biological Sciences
T1  - Uptake of metals and metalloids by conyza canadensis l. From a thermoelectric power plant landfill
VL  - 68
IS  - 4
SP  - 829
EP  - 835
DO  - 10.2298/ABS151011071V
ER  - 

@article{
author = "Vukojević, Vesna and Trifković, Jelena and Krgovic, Rada and Milojković-Opsenica, Dušanka and Marković, Marijana and Amaizah, Naser Ramdan R. and Mutić, Jelena",
year = "2016",
abstract = "Fourteen metals and metalloids were determined in Conyza canadensis L. harvested from the fly ash landfill of the thermoelectric power plant "Kolubara" (Serbia). Fly ash samples were collected together with the plant samples and subjected to sequential extraction according to the three-step sequential extraction scheme proposed by the Community Bureau of Reference (BCR; now the Standards, Measurements and Testing Program). The contents of metals and metalloids were determined by inductively coupled plasma optical emission spectrometry (ICP-OES) in plant root and the aboveground part and correlated with their contents in the fly ash samples. The bioconcentration factor (BCF) and translocation factors (TF) were calculated to access uptake of metals from fly ash and their translocation to the aboveground part. Results regarding As revealed that fly ash samples in the proximity of the active cassette had higher amounts of the element. Principal component analysis (PCA) showed that As had no impact on the classification of plant parts. BCF for As ranged from 1.44 to 23.8 and varied, depending on the investigated area; TF for As ranged from 0.43 to 2.61, indicating that the plant translocated As from root to shoot. In addition to As, Conyza canadensis L. exhibited efficient uptake of other metals from fly ash. According to the calculated BCF and TF, the plant retained Al, Fe and Cr in the root and translocated Zn, Cd, Cu and As from root to shoot in the course of the detoxifying process.",
publisher = "Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd",
journal = "Archives of Biological Sciences",
title = "Uptake of metals and metalloids by conyza canadensis l. From a thermoelectric power plant landfill",
volume = "68",
number = "4",
pages = "829-835",
doi = "10.2298/ABS151011071V"
}

Vukojević, V., Trifković, J., Krgovic, R., Milojković-Opsenica, D., Marković, M., Amaizah, N. R. R.,& Mutić, J.. (2016). Uptake of metals and metalloids by conyza canadensis l. From a thermoelectric power plant landfill. in Archives of Biological Sciences
Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd., 68(4), 829-835.
https://doi.org/10.2298/ABS151011071V

Vukojević V, Trifković J, Krgovic R, Milojković-Opsenica D, Marković M, Amaizah NRR, Mutić J. Uptake of metals and metalloids by conyza canadensis l. From a thermoelectric power plant landfill. in Archives of Biological Sciences. 2016;68(4):829-835.
doi:10.2298/ABS151011071V .

Vukojević, Vesna, Trifković, Jelena, Krgovic, Rada, Milojković-Opsenica, Dušanka, Marković, Marijana, Amaizah, Naser Ramdan R., Mutić, Jelena, "Uptake of metals and metalloids by conyza canadensis l. From a thermoelectric power plant landfill" in Archives of Biological Sciences, 68, no. 4 (2016):829-835,
https://doi.org/10.2298/ABS151011071V . .

TY  - JOUR
AU  - Andrić, Filip
AU  - Šegan, Sandra
AU  - Dramićanin, Aleksandra M.
AU  - Majstorović, Helena
AU  - Milojković-Opsenica, Dušanka
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3156
AB  - Soil-water partition coefficient normalized to the organic carbon.content (K-OC) is one of the crucial properties influencing the fate of organic compounds in the environment. Chromatographic methods are well established alternative for direct sorption techniques used for K-OC determination. The present work proposes reversed-phase thin-layer chromatography (RP-TLC) as a simpler, yet equally accurate method as officially recommended HPLC technique. Several TLC systems were studied including octadecyl-(RP18) and cyano-(CN) modified silica layers in combination with methanol-water and acetonitrile-water mixtures as mobile phases. In total 50 compounds of different molecular shape, size, and various ability to establish specific interactions were selected (phenols, beznodiazepines, triazine herbicides, and polyaromatic hydrocarbons). Calibration set of 29 compounds with known logK(OC) values determined by sorption experiments was used to build simple univariate calibrations, Principal Component Regression (PCR) and Partial Least Squares (PLS) models between logK(OC) and TLC retention parameters. Models exhibit good statistical performance, indicating that CN-layers contribute better to logK(OC) modeling than RP18-silica. The most promising TLC methods, officially recommended HPLC method, and four in silico estimation approaches have been compared by non-parametric Sum of Ranking Differences approach (SRD). The best estimations of logK(OC) values were achieved by simple univariate calibration of TLC retention data involving CN-silica layers and moderate content of methanol (40-50% v/v). They were ranked far well compared to the officially recommended HPLC method which was ranked in the middle. The worst estimates have been obtained from in silico computations based on octanol-water partition coefficient. Linear Solvation Energy Relationship study revealed that increased polarity of CN-layers over RP18 in combination with methanol-water mixtures is the key to better modeling of logK(OC) through significant diminishing of dipolar and proton accepting influence of the mobile phase as well as enhancing molar refractivity in excess of the chromatographic systems. (C) 2016 Elsevier B.V. All rights reserved.
PB  - Elsevier
T2  - Journal of Chromatography A
T1  - Linear modeling of the soil-water partition coefficient normalized to organic carbon content by reversed-phase thin-layer chromatography
VL  - 1458
SP  - 136
EP  - 144
DO  - 10.1016/j.chroma.2016.06.063
ER  - 

@article{
author = "Andrić, Filip and Šegan, Sandra and Dramićanin, Aleksandra M. and Majstorović, Helena and Milojković-Opsenica, Dušanka",
year = "2016",
abstract = "Soil-water partition coefficient normalized to the organic carbon.content (K-OC) is one of the crucial properties influencing the fate of organic compounds in the environment. Chromatographic methods are well established alternative for direct sorption techniques used for K-OC determination. The present work proposes reversed-phase thin-layer chromatography (RP-TLC) as a simpler, yet equally accurate method as officially recommended HPLC technique. Several TLC systems were studied including octadecyl-(RP18) and cyano-(CN) modified silica layers in combination with methanol-water and acetonitrile-water mixtures as mobile phases. In total 50 compounds of different molecular shape, size, and various ability to establish specific interactions were selected (phenols, beznodiazepines, triazine herbicides, and polyaromatic hydrocarbons). Calibration set of 29 compounds with known logK(OC) values determined by sorption experiments was used to build simple univariate calibrations, Principal Component Regression (PCR) and Partial Least Squares (PLS) models between logK(OC) and TLC retention parameters. Models exhibit good statistical performance, indicating that CN-layers contribute better to logK(OC) modeling than RP18-silica. The most promising TLC methods, officially recommended HPLC method, and four in silico estimation approaches have been compared by non-parametric Sum of Ranking Differences approach (SRD). The best estimations of logK(OC) values were achieved by simple univariate calibration of TLC retention data involving CN-silica layers and moderate content of methanol (40-50% v/v). They were ranked far well compared to the officially recommended HPLC method which was ranked in the middle. The worst estimates have been obtained from in silico computations based on octanol-water partition coefficient. Linear Solvation Energy Relationship study revealed that increased polarity of CN-layers over RP18 in combination with methanol-water mixtures is the key to better modeling of logK(OC) through significant diminishing of dipolar and proton accepting influence of the mobile phase as well as enhancing molar refractivity in excess of the chromatographic systems. (C) 2016 Elsevier B.V. All rights reserved.",
publisher = "Elsevier",
journal = "Journal of Chromatography A",
title = "Linear modeling of the soil-water partition coefficient normalized to organic carbon content by reversed-phase thin-layer chromatography",
volume = "1458",
pages = "136-144",
doi = "10.1016/j.chroma.2016.06.063"
}

Andrić, F., Šegan, S., Dramićanin, A. M., Majstorović, H.,& Milojković-Opsenica, D.. (2016). Linear modeling of the soil-water partition coefficient normalized to organic carbon content by reversed-phase thin-layer chromatography. in Journal of Chromatography A
Elsevier., 1458, 136-144.
https://doi.org/10.1016/j.chroma.2016.06.063

Andrić F, Šegan S, Dramićanin AM, Majstorović H, Milojković-Opsenica D. Linear modeling of the soil-water partition coefficient normalized to organic carbon content by reversed-phase thin-layer chromatography. in Journal of Chromatography A. 2016;1458:136-144.
doi:10.1016/j.chroma.2016.06.063 .

Andrić, Filip, Šegan, Sandra, Dramićanin, Aleksandra M., Majstorović, Helena, Milojković-Opsenica, Dušanka, "Linear modeling of the soil-water partition coefficient normalized to organic carbon content by reversed-phase thin-layer chromatography" in Journal of Chromatography A, 1458 (2016):136-144,
https://doi.org/10.1016/j.chroma.2016.06.063 . .

TY  - JOUR
AU  - Andrić, Filip
AU  - Šegan, Sandra
AU  - Dramićanin, Aleksandra
AU  - Majstorovic, Helena
AU  - Milojković-Opsenica, Dušanka
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1849
AB  - Soil-water partition coefficient normalized to the organic carbon.content (K-OC) is one of the crucial properties influencing the fate of organic compounds in the environment. Chromatographic methods are well established alternative for direct sorption techniques used for K-OC determination. The present work proposes reversed-phase thin-layer chromatography (RP-TLC) as a simpler, yet equally accurate method as officially recommended HPLC technique. Several TLC systems were studied including octadecyl-(RP18) and cyano-(CN) modified silica layers in combination with methanol-water and acetonitrile-water mixtures as mobile phases. In total 50 compounds of different molecular shape, size, and various ability to establish specific interactions were selected (phenols, beznodiazepines, triazine herbicides, and polyaromatic hydrocarbons). Calibration set of 29 compounds with known logK(OC) values determined by sorption experiments was used to build simple univariate calibrations, Principal Component Regression (PCR) and Partial Least Squares (PLS) models between logK(OC) and TLC retention parameters. Models exhibit good statistical performance, indicating that CN-layers contribute better to logK(OC) modeling than RP18-silica. The most promising TLC methods, officially recommended HPLC method, and four in silico estimation approaches have been compared by non-parametric Sum of Ranking Differences approach (SRD). The best estimations of logK(OC) values were achieved by simple univariate calibration of TLC retention data involving CN-silica layers and moderate content of methanol (40-50% v/v). They were ranked far well compared to the officially recommended HPLC method which was ranked in the middle. The worst estimates have been obtained from in silico computations based on octanol-water partition coefficient. Linear Solvation Energy Relationship study revealed that increased polarity of CN-layers over RP18 in combination with methanol-water mixtures is the key to better modeling of logK(OC) through significant diminishing of dipolar and proton accepting influence of the mobile phase as well as enhancing molar refractivity in excess of the chromatographic systems.
PB  - Elsevier
T2  - Journal of Chromatography A
T1  - Linear modeling of the soil-water partition coefficient normalized to organic carbon content by reversed-phase thin-layer chromatography
VL  - 1458
SP  - 136
EP  - 144
DO  - 10.1016/j.chroma.2016.06.063
ER  - 

@article{
author = "Andrić, Filip and Šegan, Sandra and Dramićanin, Aleksandra and Majstorovic, Helena and Milojković-Opsenica, Dušanka",
year = "2016",
abstract = "Soil-water partition coefficient normalized to the organic carbon.content (K-OC) is one of the crucial properties influencing the fate of organic compounds in the environment. Chromatographic methods are well established alternative for direct sorption techniques used for K-OC determination. The present work proposes reversed-phase thin-layer chromatography (RP-TLC) as a simpler, yet equally accurate method as officially recommended HPLC technique. Several TLC systems were studied including octadecyl-(RP18) and cyano-(CN) modified silica layers in combination with methanol-water and acetonitrile-water mixtures as mobile phases. In total 50 compounds of different molecular shape, size, and various ability to establish specific interactions were selected (phenols, beznodiazepines, triazine herbicides, and polyaromatic hydrocarbons). Calibration set of 29 compounds with known logK(OC) values determined by sorption experiments was used to build simple univariate calibrations, Principal Component Regression (PCR) and Partial Least Squares (PLS) models between logK(OC) and TLC retention parameters. Models exhibit good statistical performance, indicating that CN-layers contribute better to logK(OC) modeling than RP18-silica. The most promising TLC methods, officially recommended HPLC method, and four in silico estimation approaches have been compared by non-parametric Sum of Ranking Differences approach (SRD). The best estimations of logK(OC) values were achieved by simple univariate calibration of TLC retention data involving CN-silica layers and moderate content of methanol (40-50% v/v). They were ranked far well compared to the officially recommended HPLC method which was ranked in the middle. The worst estimates have been obtained from in silico computations based on octanol-water partition coefficient. Linear Solvation Energy Relationship study revealed that increased polarity of CN-layers over RP18 in combination with methanol-water mixtures is the key to better modeling of logK(OC) through significant diminishing of dipolar and proton accepting influence of the mobile phase as well as enhancing molar refractivity in excess of the chromatographic systems.",
publisher = "Elsevier",
journal = "Journal of Chromatography A",
title = "Linear modeling of the soil-water partition coefficient normalized to organic carbon content by reversed-phase thin-layer chromatography",
volume = "1458",
pages = "136-144",
doi = "10.1016/j.chroma.2016.06.063"
}

Andrić, F., Šegan, S., Dramićanin, A., Majstorovic, H.,& Milojković-Opsenica, D.. (2016). Linear modeling of the soil-water partition coefficient normalized to organic carbon content by reversed-phase thin-layer chromatography. in Journal of Chromatography A
Elsevier., 1458, 136-144.
https://doi.org/10.1016/j.chroma.2016.06.063

Andrić F, Šegan S, Dramićanin A, Majstorovic H, Milojković-Opsenica D. Linear modeling of the soil-water partition coefficient normalized to organic carbon content by reversed-phase thin-layer chromatography. in Journal of Chromatography A. 2016;1458:136-144.
doi:10.1016/j.chroma.2016.06.063 .

Andrić, Filip, Šegan, Sandra, Dramićanin, Aleksandra, Majstorovic, Helena, Milojković-Opsenica, Dušanka, "Linear modeling of the soil-water partition coefficient normalized to organic carbon content by reversed-phase thin-layer chromatography" in Journal of Chromatography A, 1458 (2016):136-144,
https://doi.org/10.1016/j.chroma.2016.06.063 . .

TY  - JOUR
AU  - Andrić, Filip
AU  - Šegan, Sandra
AU  - Tešić, Živoslav
AU  - Milojković-Opsenica, Dušanka
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1861
AB  - The soil-water partition coefficient normalized to the soil organic carbon content (K-OC) is one of essential properties governing the fate of organic chemicals in the soil-water compartment. It is a parameter of utmost importance when it comes to the removal of toxic organics in the waste water management facilities or placing a new chemical on the market. Since direct determination of K-OC based on tests involving soil-sorption measurements are expensive, time-consuming, tedious, and subject to different experimental difficulties and artifacts, different alternative methods have been developed for rapid indirect experimental determination and estimation of K-OC values. In the scope of the present work, we provide critical and historical overview of development and application of the main chromatographic methods, both high-performance liquid chromatography and thin-layer chromatographic techniques for indirect experimental determination of the soil-water partition coefficient as well as mobility assessment of environmentally important compounds in the soil. Chromatographic methods proved to be promising in this field, which is clearly demonstrated by their implementation in official guidelines for testing the chemicals.
PB  - Taylor & Francis Inc, Philadelphia
T2  - Journal of Liquid Chromatography & Related Technologies
T1  - Chromatographic methods in determination of the soil-water partition coefficient
VL  - 39
IS  - 5-6
SP  - 249
EP  - 256
DO  - 10.1080/10826076.2016.1163173
ER  - 

@article{
author = "Andrić, Filip and Šegan, Sandra and Tešić, Živoslav and Milojković-Opsenica, Dušanka",
year = "2016",
abstract = "The soil-water partition coefficient normalized to the soil organic carbon content (K-OC) is one of essential properties governing the fate of organic chemicals in the soil-water compartment. It is a parameter of utmost importance when it comes to the removal of toxic organics in the waste water management facilities or placing a new chemical on the market. Since direct determination of K-OC based on tests involving soil-sorption measurements are expensive, time-consuming, tedious, and subject to different experimental difficulties and artifacts, different alternative methods have been developed for rapid indirect experimental determination and estimation of K-OC values. In the scope of the present work, we provide critical and historical overview of development and application of the main chromatographic methods, both high-performance liquid chromatography and thin-layer chromatographic techniques for indirect experimental determination of the soil-water partition coefficient as well as mobility assessment of environmentally important compounds in the soil. Chromatographic methods proved to be promising in this field, which is clearly demonstrated by their implementation in official guidelines for testing the chemicals.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Journal of Liquid Chromatography & Related Technologies",
title = "Chromatographic methods in determination of the soil-water partition coefficient",
volume = "39",
number = "5-6",
pages = "249-256",
doi = "10.1080/10826076.2016.1163173"
}

Andrić, F., Šegan, S., Tešić, Ž.,& Milojković-Opsenica, D.. (2016). Chromatographic methods in determination of the soil-water partition coefficient. in Journal of Liquid Chromatography & Related Technologies
Taylor & Francis Inc, Philadelphia., 39(5-6), 249-256.
https://doi.org/10.1080/10826076.2016.1163173

Andrić F, Šegan S, Tešić Ž, Milojković-Opsenica D. Chromatographic methods in determination of the soil-water partition coefficient. in Journal of Liquid Chromatography & Related Technologies. 2016;39(5-6):249-256.
doi:10.1080/10826076.2016.1163173 .

Andrić, Filip, Šegan, Sandra, Tešić, Živoslav, Milojković-Opsenica, Dušanka, "Chromatographic methods in determination of the soil-water partition coefficient" in Journal of Liquid Chromatography & Related Technologies, 39, no. 5-6 (2016):249-256,
https://doi.org/10.1080/10826076.2016.1163173 . .

TY  - JOUR
AU  - Tosti, Tomislav
AU  - Šegan, Sandra
AU  - Milić, Dragana
AU  - Radoičić, Aleksandra D.
AU  - Tešić, Živoslav
AU  - Milojković-Opsenica, Dušanka
PY  - 2015
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1646
AB  - Unmodified silica gel in combination with two mobile phases, acetone-n-hexane and acetonitrile-dichloromethane, was used in order to evaluate the capability of normal-phase (NP) chromatography on bare silica gel in estimation of lipophilicity of some polyoxygenated steroids. Soczewinski equation coefficients were employed as a measure of lipophilicity. The R-M(0) values obtained in NP-systems were correlated with those derived by extrapolation from reversed-phase (RP) systems. In addition, retention data, i.e., lipophilicity parameters determined in NP systems were compared with logP values calculated by use of several commercial computer programs. The results showed that chromatographic parameters R-M(0), and m obtained in NP system consisted of silica gel as stationary phase and acetone-n-hexane as mobile phase, are acceptable as the measures of lipophilicity of polyoxygenated steroids. The mechanism of retention was discussed.
PB  - Taylor & Francis Inc, Philadelphia
T2  - Journal of Liquid Chromatography & Related Technologies
T1  - Estimation of Lipophilicity of Some Polyoxygenated Steroids by the Means of Normal-Phase Thin-Layer Chromatography
VL  - 38
IS  - 11
SP  - 1097
EP  - 1103
DO  - 10.1080/10826076.2015.1028287
ER  - 

@article{
author = "Tosti, Tomislav and Šegan, Sandra and Milić, Dragana and Radoičić, Aleksandra D. and Tešić, Živoslav and Milojković-Opsenica, Dušanka",
year = "2015",
abstract = "Unmodified silica gel in combination with two mobile phases, acetone-n-hexane and acetonitrile-dichloromethane, was used in order to evaluate the capability of normal-phase (NP) chromatography on bare silica gel in estimation of lipophilicity of some polyoxygenated steroids. Soczewinski equation coefficients were employed as a measure of lipophilicity. The R-M(0) values obtained in NP-systems were correlated with those derived by extrapolation from reversed-phase (RP) systems. In addition, retention data, i.e., lipophilicity parameters determined in NP systems were compared with logP values calculated by use of several commercial computer programs. The results showed that chromatographic parameters R-M(0), and m obtained in NP system consisted of silica gel as stationary phase and acetone-n-hexane as mobile phase, are acceptable as the measures of lipophilicity of polyoxygenated steroids. The mechanism of retention was discussed.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Journal of Liquid Chromatography & Related Technologies",
title = "Estimation of Lipophilicity of Some Polyoxygenated Steroids by the Means of Normal-Phase Thin-Layer Chromatography",
volume = "38",
number = "11",
pages = "1097-1103",
doi = "10.1080/10826076.2015.1028287"
}

Tosti, T., Šegan, S., Milić, D., Radoičić, A. D., Tešić, Ž.,& Milojković-Opsenica, D.. (2015). Estimation of Lipophilicity of Some Polyoxygenated Steroids by the Means of Normal-Phase Thin-Layer Chromatography. in Journal of Liquid Chromatography & Related Technologies
Taylor & Francis Inc, Philadelphia., 38(11), 1097-1103.
https://doi.org/10.1080/10826076.2015.1028287

Tosti T, Šegan S, Milić D, Radoičić AD, Tešić Ž, Milojković-Opsenica D. Estimation of Lipophilicity of Some Polyoxygenated Steroids by the Means of Normal-Phase Thin-Layer Chromatography. in Journal of Liquid Chromatography & Related Technologies. 2015;38(11):1097-1103.
doi:10.1080/10826076.2015.1028287 .

Tosti, Tomislav, Šegan, Sandra, Milić, Dragana, Radoičić, Aleksandra D., Tešić, Živoslav, Milojković-Opsenica, Dušanka, "Estimation of Lipophilicity of Some Polyoxygenated Steroids by the Means of Normal-Phase Thin-Layer Chromatography" in Journal of Liquid Chromatography & Related Technologies, 38, no. 11 (2015):1097-1103,
https://doi.org/10.1080/10826076.2015.1028287 . .

TY  - JOUR
AU  - Tosti, Tomislav
AU  - Šegan, Sandra
AU  - Milić, Dragana
AU  - Radoičić, Aleksandra D.
AU  - Tešić, Živoslav
AU  - Milojković-Opsenica, Dušanka
PY  - 2015
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3126
AB  - Unmodified silica gel in combination with two mobile phases, acetone-n-hexane and acetonitrile-dichloromethane, was used in order to evaluate the capability of normal-phase (NP) chromatography on bare silica gel in estimation of lipophilicity of some polyoxygenated steroids. Soczewinski equation coefficients were employed as a measure of lipophilicity. The R-M(0) values obtained in NP-systems were correlated with those derived by extrapolation from reversed-phase (RP) systems. In addition, retention data, i.e., lipophilicity parameters determined in NP systems were compared with logP values calculated by use of several commercial computer programs. The results showed that chromatographic parameters R-M(0), and m obtained in NP system consisted of silica gel as stationary phase and acetone-n-hexane as mobile phase, are acceptable as the measures of lipophilicity of polyoxygenated steroids. The mechanism of retention was discussed.
PB  - Taylor & Francis Inc, Philadelphia
T2  - Journal of Liquid Chromatography & Related Technologies
T1  - Estimation of Lipophilicity of Some Polyoxygenated Steroids by the Means of Normal-Phase Thin-Layer Chromatography
VL  - 38
IS  - 11
SP  - 1097
EP  - 1103
DO  - 10.1080/10826076.2015.1028287
ER  - 

@article{
author = "Tosti, Tomislav and Šegan, Sandra and Milić, Dragana and Radoičić, Aleksandra D. and Tešić, Živoslav and Milojković-Opsenica, Dušanka",
year = "2015",
abstract = "Unmodified silica gel in combination with two mobile phases, acetone-n-hexane and acetonitrile-dichloromethane, was used in order to evaluate the capability of normal-phase (NP) chromatography on bare silica gel in estimation of lipophilicity of some polyoxygenated steroids. Soczewinski equation coefficients were employed as a measure of lipophilicity. The R-M(0) values obtained in NP-systems were correlated with those derived by extrapolation from reversed-phase (RP) systems. In addition, retention data, i.e., lipophilicity parameters determined in NP systems were compared with logP values calculated by use of several commercial computer programs. The results showed that chromatographic parameters R-M(0), and m obtained in NP system consisted of silica gel as stationary phase and acetone-n-hexane as mobile phase, are acceptable as the measures of lipophilicity of polyoxygenated steroids. The mechanism of retention was discussed.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Journal of Liquid Chromatography & Related Technologies",
title = "Estimation of Lipophilicity of Some Polyoxygenated Steroids by the Means of Normal-Phase Thin-Layer Chromatography",
volume = "38",
number = "11",
pages = "1097-1103",
doi = "10.1080/10826076.2015.1028287"
}

Tosti, T., Šegan, S., Milić, D., Radoičić, A. D., Tešić, Ž.,& Milojković-Opsenica, D.. (2015). Estimation of Lipophilicity of Some Polyoxygenated Steroids by the Means of Normal-Phase Thin-Layer Chromatography. in Journal of Liquid Chromatography & Related Technologies
Taylor & Francis Inc, Philadelphia., 38(11), 1097-1103.
https://doi.org/10.1080/10826076.2015.1028287

Tosti T, Šegan S, Milić D, Radoičić AD, Tešić Ž, Milojković-Opsenica D. Estimation of Lipophilicity of Some Polyoxygenated Steroids by the Means of Normal-Phase Thin-Layer Chromatography. in Journal of Liquid Chromatography & Related Technologies. 2015;38(11):1097-1103.
doi:10.1080/10826076.2015.1028287 .

Tosti, Tomislav, Šegan, Sandra, Milić, Dragana, Radoičić, Aleksandra D., Tešić, Živoslav, Milojković-Opsenica, Dušanka, "Estimation of Lipophilicity of Some Polyoxygenated Steroids by the Means of Normal-Phase Thin-Layer Chromatography" in Journal of Liquid Chromatography & Related Technologies, 38, no. 11 (2015):1097-1103,
https://doi.org/10.1080/10826076.2015.1028287 . .

TY  - JOUR
AU  - Krgovic, Rada
AU  - Trifković, Jelena
AU  - Milojković-Opsenica, Dušanka
AU  - Manojlović, Dragan
AU  - Marković, Marijana
AU  - Mutić, Jelena
PY  - 2015
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1625
AB  - The objectives of this study were to determine the concentrations of Pb, Cd, As, Cr, Cu, Co, Ni, Zn, Ba, Fe, Al and Ag in Erigeron canadensis L. growing on fly ash landfill of power plant "Kolubara", Serbia. The content of each element was determined in every part of plant separately (root, stalk and inflorescence) and correlated with the content of elements in each phase of sequential extraction of fly ash. In order to ambiguously select the factors that are able to decidedly characterize the particular part of plant, principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were performed. The bioconcentration factors and translocation factors for each metal were calculated in order to determine the feasibility of the use of plant E. canadensis L. for phytoremediation purpose. There were strong positive correlations between metals in every part of plant samples, and metals from pseudo total form of sequential extraction indicate that the bioavailability of elements in fly ash is similarly correlated with total form. Retained Al, Fe, Cr and Co in the root indicate its suitability for phytostabilization. This plant takes up Cd and Zn from the soil (bioconcentration factors (BCFs) greater than 1), transporting them through the stalk into the inflorescence (translocation factors (TFs) higher than 1). Regarding its dominance in vegetation cover and abundance, E. canadensis L. can be considered adequate for phytoextraction of Cd and Zn from coal ash landfills at Kolubara.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Science and Pollution Research
T1  - Phytoextraction of metals by Erigeron canadensis L. from fly ash landfill of power plant "Kolubara"
VL  - 22
IS  - 14
SP  - 10506
EP  - 10515
DO  - 10.1007/s11356-015-4192-5
ER  - 

@article{
author = "Krgovic, Rada and Trifković, Jelena and Milojković-Opsenica, Dušanka and Manojlović, Dragan and Marković, Marijana and Mutić, Jelena",
year = "2015",
abstract = "The objectives of this study were to determine the concentrations of Pb, Cd, As, Cr, Cu, Co, Ni, Zn, Ba, Fe, Al and Ag in Erigeron canadensis L. growing on fly ash landfill of power plant "Kolubara", Serbia. The content of each element was determined in every part of plant separately (root, stalk and inflorescence) and correlated with the content of elements in each phase of sequential extraction of fly ash. In order to ambiguously select the factors that are able to decidedly characterize the particular part of plant, principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were performed. The bioconcentration factors and translocation factors for each metal were calculated in order to determine the feasibility of the use of plant E. canadensis L. for phytoremediation purpose. There were strong positive correlations between metals in every part of plant samples, and metals from pseudo total form of sequential extraction indicate that the bioavailability of elements in fly ash is similarly correlated with total form. Retained Al, Fe, Cr and Co in the root indicate its suitability for phytostabilization. This plant takes up Cd and Zn from the soil (bioconcentration factors (BCFs) greater than 1), transporting them through the stalk into the inflorescence (translocation factors (TFs) higher than 1). Regarding its dominance in vegetation cover and abundance, E. canadensis L. can be considered adequate for phytoextraction of Cd and Zn from coal ash landfills at Kolubara.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Science and Pollution Research",
title = "Phytoextraction of metals by Erigeron canadensis L. from fly ash landfill of power plant "Kolubara"",
volume = "22",
number = "14",
pages = "10506-10515",
doi = "10.1007/s11356-015-4192-5"
}

Krgovic, R., Trifković, J., Milojković-Opsenica, D., Manojlović, D., Marković, M.,& Mutić, J.. (2015). Phytoextraction of metals by Erigeron canadensis L. from fly ash landfill of power plant "Kolubara". in Environmental Science and Pollution Research
Springer Heidelberg, Heidelberg., 22(14), 10506-10515.
https://doi.org/10.1007/s11356-015-4192-5

Krgovic R, Trifković J, Milojković-Opsenica D, Manojlović D, Marković M, Mutić J. Phytoextraction of metals by Erigeron canadensis L. from fly ash landfill of power plant "Kolubara". in Environmental Science and Pollution Research. 2015;22(14):10506-10515.
doi:10.1007/s11356-015-4192-5 .

Krgovic, Rada, Trifković, Jelena, Milojković-Opsenica, Dušanka, Manojlović, Dragan, Marković, Marijana, Mutić, Jelena, "Phytoextraction of metals by Erigeron canadensis L. from fly ash landfill of power plant "Kolubara"" in Environmental Science and Pollution Research, 22, no. 14 (2015):10506-10515,
https://doi.org/10.1007/s11356-015-4192-5 . .

TY  - JOUR
AU  - Stanojevic, Marija
AU  - Trifković, Jelena
AU  - Fotiric-Aksic, Milica M
AU  - Rakonjac, Vera
AU  - Nikolic, Dragan
AU  - Šegan, Sandra
AU  - Milojković-Opsenica, Dušanka
PY  - 2015
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1786
AB  - Large amounts of fruit seeds, especially peach, are discarded annually in juice or conserve producing industries which is a potential waste of valuable resource and serious disposal problem. Regarding the fact that peach seeds can be obtained as a byproduct from processing companies their exploitation should be greater and, consequently more information of cultivars' kernels and their composition is required. A total of 25 samples of kernels from various peach germplasm (including commercial cultivars, perspective hybrids and vineyard peach accessions) differing in origin and ripening time were characterized by evaluation of their sugar composition. Twenty characteristic carbohydrates and sugar alcohols were determined and quantified using high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC/PAD). Sucrose, glucose and fructose are the most important sugars in peach kernels similar to other representatives of the Rosaceae family. Also, high amounts of sugars in seeds of promising hybrids implies that through conventional breeding programs peach kernels with high sugar content can be obtained. In addition, by the means of several pattern recognition methods the variables that discriminate peach kernels arising from diverse germplasm and different stage of maturity were identified and successful models for further prediction were developed. Sugars such as ribose, trehalose, arabinose, galactitol, fructose, maltose, sorbitol, sucrose, iso-maltotriose were marked as most important for such discrimination.
PB  - Springer, Dordrecht
T2  - Plant Foods For Human Nutrition
T1  - Sugar Profile of Kernels as a Marker of Origin and Ripening Time of Peach (Prunus persicae L.)
VL  - 70
IS  - 4
SP  - 433
EP  - 440
DO  - 10.1007/s11130-015-0515-4
ER  - 

@article{
author = "Stanojevic, Marija and Trifković, Jelena and Fotiric-Aksic, Milica M and Rakonjac, Vera and Nikolic, Dragan and Šegan, Sandra and Milojković-Opsenica, Dušanka",
year = "2015",
abstract = "Large amounts of fruit seeds, especially peach, are discarded annually in juice or conserve producing industries which is a potential waste of valuable resource and serious disposal problem. Regarding the fact that peach seeds can be obtained as a byproduct from processing companies their exploitation should be greater and, consequently more information of cultivars' kernels and their composition is required. A total of 25 samples of kernels from various peach germplasm (including commercial cultivars, perspective hybrids and vineyard peach accessions) differing in origin and ripening time were characterized by evaluation of their sugar composition. Twenty characteristic carbohydrates and sugar alcohols were determined and quantified using high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC/PAD). Sucrose, glucose and fructose are the most important sugars in peach kernels similar to other representatives of the Rosaceae family. Also, high amounts of sugars in seeds of promising hybrids implies that through conventional breeding programs peach kernels with high sugar content can be obtained. In addition, by the means of several pattern recognition methods the variables that discriminate peach kernels arising from diverse germplasm and different stage of maturity were identified and successful models for further prediction were developed. Sugars such as ribose, trehalose, arabinose, galactitol, fructose, maltose, sorbitol, sucrose, iso-maltotriose were marked as most important for such discrimination.",
publisher = "Springer, Dordrecht",
journal = "Plant Foods For Human Nutrition",
title = "Sugar Profile of Kernels as a Marker of Origin and Ripening Time of Peach (Prunus persicae L.)",
volume = "70",
number = "4",
pages = "433-440",
doi = "10.1007/s11130-015-0515-4"
}

Stanojevic, M., Trifković, J., Fotiric-Aksic, M. M., Rakonjac, V., Nikolic, D., Šegan, S.,& Milojković-Opsenica, D.. (2015). Sugar Profile of Kernels as a Marker of Origin and Ripening Time of Peach (Prunus persicae L.). in Plant Foods For Human Nutrition
Springer, Dordrecht., 70(4), 433-440.
https://doi.org/10.1007/s11130-015-0515-4

Stanojevic M, Trifković J, Fotiric-Aksic MM, Rakonjac V, Nikolic D, Šegan S, Milojković-Opsenica D. Sugar Profile of Kernels as a Marker of Origin and Ripening Time of Peach (Prunus persicae L.). in Plant Foods For Human Nutrition. 2015;70(4):433-440.
doi:10.1007/s11130-015-0515-4 .

Stanojevic, Marija, Trifković, Jelena, Fotiric-Aksic, Milica M, Rakonjac, Vera, Nikolic, Dragan, Šegan, Sandra, Milojković-Opsenica, Dušanka, "Sugar Profile of Kernels as a Marker of Origin and Ripening Time of Peach (Prunus persicae L.)" in Plant Foods For Human Nutrition, 70, no. 4 (2015):433-440,
https://doi.org/10.1007/s11130-015-0515-4 . .

TY  - JOUR
AU  - Jurica, Karlo
AU  - Karaconji, Irena Brcic
AU  - Šegan, Sandra
AU  - Milojković-Opsenica, Dušanka
AU  - Kremer, Dario
PY  - 2015
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1792
AB  - The phenolic glycoside arbutin and its metabolite with uroantiseptic activity hydroquinone occur naturally in the leaves of various medicinal plants and spices. In this study, an extraction procedure coupled with gas chromatography-mass spectrometry (GC-MS) was developed to determine arbutin and hydroquinone content in strawberry tree (Arbutus unedo L., Ericaceae) leaves. The method showed good linearity (R-2>0.9987) in the tested concentration range (0.5-200 mu g mL(-1)), as well as good precision (RSD LT 5 %), analytical recovery (96.2-98.0 %), and sensitivity (limit of detection=0.009 and 0.004 mu g mL(-1) for arbutin and hydroquinone, respectively). The results obtained by the validated GC-MS method corresponded well to those obtained by high performance liquid chromatography (HPLC) method. The proposed method was then applied for determining arbutin and hydroquinone content in methanolic leaf extracts. The amount of arbutin in the leaves collected on the island of Kolocep (6.82 mg g(-1) dry weight) was found to be higher (t(paired)=43.57, t(c)=2.92) in comparison to the amount of arbutin in the leaves collected on the island of Mali Losinj (2.75 mg g(-1) dry weight). Hydroquinone was not detected in any of the samples. The analytical features of the proposed GC-MS method demonstrated that arbutin and hydroquinone could be determined alternatively by gas chromatography. Due to its wide concentration range, the method could also be suitable for arbutin and hydroquinone analysis in leaves of other plant families (Rosaceae, Lamiaceae, etc.).
PB  - Inst Medical Research & Occupational Health, Zagreb
T2  - Arhiv Za Higijenu Rada I Toksikologiju-Archives of Industrial Hygiene and Toxicology
T1  - Quantitative analysis of arbutin and hydroquinone in strawberry tree (Arbutus unedo L., Ericaceae) leaves by gas chromatography-mass spectrometry
VL  - 66
IS  - 3
SP  - 197
EP  - 202
DO  - 10.1515/aiht-2015-66-2696
ER  - 

@article{
author = "Jurica, Karlo and Karaconji, Irena Brcic and Šegan, Sandra and Milojković-Opsenica, Dušanka and Kremer, Dario",
year = "2015",
abstract = "The phenolic glycoside arbutin and its metabolite with uroantiseptic activity hydroquinone occur naturally in the leaves of various medicinal plants and spices. In this study, an extraction procedure coupled with gas chromatography-mass spectrometry (GC-MS) was developed to determine arbutin and hydroquinone content in strawberry tree (Arbutus unedo L., Ericaceae) leaves. The method showed good linearity (R-2>0.9987) in the tested concentration range (0.5-200 mu g mL(-1)), as well as good precision (RSD LT 5 %), analytical recovery (96.2-98.0 %), and sensitivity (limit of detection=0.009 and 0.004 mu g mL(-1) for arbutin and hydroquinone, respectively). The results obtained by the validated GC-MS method corresponded well to those obtained by high performance liquid chromatography (HPLC) method. The proposed method was then applied for determining arbutin and hydroquinone content in methanolic leaf extracts. The amount of arbutin in the leaves collected on the island of Kolocep (6.82 mg g(-1) dry weight) was found to be higher (t(paired)=43.57, t(c)=2.92) in comparison to the amount of arbutin in the leaves collected on the island of Mali Losinj (2.75 mg g(-1) dry weight). Hydroquinone was not detected in any of the samples. The analytical features of the proposed GC-MS method demonstrated that arbutin and hydroquinone could be determined alternatively by gas chromatography. Due to its wide concentration range, the method could also be suitable for arbutin and hydroquinone analysis in leaves of other plant families (Rosaceae, Lamiaceae, etc.).",
publisher = "Inst Medical Research & Occupational Health, Zagreb",
journal = "Arhiv Za Higijenu Rada I Toksikologiju-Archives of Industrial Hygiene and Toxicology",
title = "Quantitative analysis of arbutin and hydroquinone in strawberry tree (Arbutus unedo L., Ericaceae) leaves by gas chromatography-mass spectrometry",
volume = "66",
number = "3",
pages = "197-202",
doi = "10.1515/aiht-2015-66-2696"
}

Jurica, K., Karaconji, I. B., Šegan, S., Milojković-Opsenica, D.,& Kremer, D.. (2015). Quantitative analysis of arbutin and hydroquinone in strawberry tree (Arbutus unedo L., Ericaceae) leaves by gas chromatography-mass spectrometry. in Arhiv Za Higijenu Rada I Toksikologiju-Archives of Industrial Hygiene and Toxicology
Inst Medical Research & Occupational Health, Zagreb., 66(3), 197-202.
https://doi.org/10.1515/aiht-2015-66-2696

Jurica K, Karaconji IB, Šegan S, Milojković-Opsenica D, Kremer D. Quantitative analysis of arbutin and hydroquinone in strawberry tree (Arbutus unedo L., Ericaceae) leaves by gas chromatography-mass spectrometry. in Arhiv Za Higijenu Rada I Toksikologiju-Archives of Industrial Hygiene and Toxicology. 2015;66(3):197-202.
doi:10.1515/aiht-2015-66-2696 .

Jurica, Karlo, Karaconji, Irena Brcic, Šegan, Sandra, Milojković-Opsenica, Dušanka, Kremer, Dario, "Quantitative analysis of arbutin and hydroquinone in strawberry tree (Arbutus unedo L., Ericaceae) leaves by gas chromatography-mass spectrometry" in Arhiv Za Higijenu Rada I Toksikologiju-Archives of Industrial Hygiene and Toxicology, 66, no. 3 (2015):197-202,
https://doi.org/10.1515/aiht-2015-66-2696 . .

TY  - JOUR
AU  - Šegan, Sandra
AU  - Terzić Jovanović, Nataša
AU  - Milojković-Opsenica, Dušanka
AU  - Trifković, Jelena
AU  - Šolaja, Bogdan
AU  - Opsenica, Dejan
PY  - 2014
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1599
AB  - The chromatographic behavior of mixed 1,2,4,5-tetraoxanes, cholic and deoxycholic acid derivatives with distinct biological activity, was examined by high-performance thin-layer chromatography in order to correlate their structure and retention. Chromatographic systems were consisted of RP-18 or CN-silica as stationary phase, and binary mixtures of water with methanol, dioxane or acetone as mobile phase. Based on the respective retentions, the lipophilicity of the investigated compounds was determined. Multiple linear regression and partial least squares have been used to select variables that best describe the behavior of the investigated compounds in chromatographic systems and to quantify influences of most important parameters. The validation and cross-validation of the QSRR model suggest its applicability for prediction and understanding of retention of congeners. The models indicate the importance of nonpolar properties of the solutes and their ability for hydrophobic interactions, as well as the importance of proton donating abilities, hydrophilic and,pi interactions pointing out on that way the possible separation mechanism in the studied chromatographic systems. Observed correlations between structure and biological activity of mixed 1,2,4,5-tetraoxanes, indicate that the antimalarial activity against W2 and D6 Plasmodium falciparum strains, is governed by hydrophobic feature (measured with lipophilicity parameter), hydrophilic feature (measured with HLB, %HS, HB and HBA descriptors), and electronic feature (HOMO).
PB  - Elsevier
T2  - Journal of Pharmaceutical and Biomedical Analysis
T1  - Correlation study of retention data and antimalarial activity of 1,2,4,5-mixed tetraoxanes with their molecular structure descriptors and LSER parameters
VL  - 97
SP  - 178
EP  - 183
DO  - 10.1016/j.jpba.2014.04.029
ER  - 

@article{
author = "Šegan, Sandra and Terzić Jovanović, Nataša and Milojković-Opsenica, Dušanka and Trifković, Jelena and Šolaja, Bogdan and Opsenica, Dejan",
year = "2014",
abstract = "The chromatographic behavior of mixed 1,2,4,5-tetraoxanes, cholic and deoxycholic acid derivatives with distinct biological activity, was examined by high-performance thin-layer chromatography in order to correlate their structure and retention. Chromatographic systems were consisted of RP-18 or CN-silica as stationary phase, and binary mixtures of water with methanol, dioxane or acetone as mobile phase. Based on the respective retentions, the lipophilicity of the investigated compounds was determined. Multiple linear regression and partial least squares have been used to select variables that best describe the behavior of the investigated compounds in chromatographic systems and to quantify influences of most important parameters. The validation and cross-validation of the QSRR model suggest its applicability for prediction and understanding of retention of congeners. The models indicate the importance of nonpolar properties of the solutes and their ability for hydrophobic interactions, as well as the importance of proton donating abilities, hydrophilic and,pi interactions pointing out on that way the possible separation mechanism in the studied chromatographic systems. Observed correlations between structure and biological activity of mixed 1,2,4,5-tetraoxanes, indicate that the antimalarial activity against W2 and D6 Plasmodium falciparum strains, is governed by hydrophobic feature (measured with lipophilicity parameter), hydrophilic feature (measured with HLB, %HS, HB and HBA descriptors), and electronic feature (HOMO).",
publisher = "Elsevier",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "Correlation study of retention data and antimalarial activity of 1,2,4,5-mixed tetraoxanes with their molecular structure descriptors and LSER parameters",
volume = "97",
pages = "178-183",
doi = "10.1016/j.jpba.2014.04.029"
}

Šegan, S., Terzić Jovanović, N., Milojković-Opsenica, D., Trifković, J., Šolaja, B.,& Opsenica, D.. (2014). Correlation study of retention data and antimalarial activity of 1,2,4,5-mixed tetraoxanes with their molecular structure descriptors and LSER parameters. in Journal of Pharmaceutical and Biomedical Analysis
Elsevier., 97, 178-183.
https://doi.org/10.1016/j.jpba.2014.04.029

Šegan S, Terzić Jovanović N, Milojković-Opsenica D, Trifković J, Šolaja B, Opsenica D. Correlation study of retention data and antimalarial activity of 1,2,4,5-mixed tetraoxanes with their molecular structure descriptors and LSER parameters. in Journal of Pharmaceutical and Biomedical Analysis. 2014;97:178-183.
doi:10.1016/j.jpba.2014.04.029 .

Šegan, Sandra, Terzić Jovanović, Nataša, Milojković-Opsenica, Dušanka, Trifković, Jelena, Šolaja, Bogdan, Opsenica, Dejan, "Correlation study of retention data and antimalarial activity of 1,2,4,5-mixed tetraoxanes with their molecular structure descriptors and LSER parameters" in Journal of Pharmaceutical and Biomedical Analysis, 97 (2014):178-183,
https://doi.org/10.1016/j.jpba.2014.04.029 . .

TY  - JOUR
AU  - Šegan, Sandra
AU  - Terzić-Jovanović, Nataša
AU  - Milojković-Opsenica, Dušanka
AU  - Trifković, Jelena
AU  - Šolaja, Bogdan
AU  - Opsenica, Dejan
PY  - 2014
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3166
AB  - The chromatographic behavior of mixed 1,2,4,5-tetraoxanes, cholic and deoxycholic acid derivatives with distinct biological activity, was examined by high-performance thin-layer chromatography in order to correlate their structure and retention. Chromatographic systems were consisted of RP-18 or CN-silica as stationary phase, and binary mixtures of water with methanol, dioxane or acetone as mobile phase. Based on the respective retentions, the lipophilicity of the investigated compounds was determined. Multiple linear regression and partial least squares have been used to select variables that best describe the behavior of the investigated compounds in chromatographic systems and to quantify influences of most important parameters. The validation and cross-validation of the QSRR model suggest its applicability for prediction and understanding of retention of congeners. The models indicate the importance of nonpolar properties of the solutes and their ability for hydrophobic interactions, as well as the importance of proton donating abilities, hydrophilic and,pi interactions pointing out on that way the possible separation mechanism in the studied chromatographic systems. Observed correlations between structure and biological activity of mixed 1,2,4,5-tetraoxanes, indicate that the antimalarial activity against W2 and D6 Plasmodium falciparum strains, is governed by hydrophobic feature (measured with lipophilicity parameter), hydrophilic feature (measured with HLB, %HS, HB and HBA descriptors), and electronic feature (HOMO).
PB  - Elsevier
T2  - Journal of Pharmaceutical and Biomedical Analysis
T1  - Correlation study of retention data and antimalarial activity of 1,2,4,5-mixed tetraoxanes with their molecular structure descriptors and LSER parameters
VL  - 97
SP  - 178
EP  - 183
DO  - 10.1016/j.jpba.2014.04.029
ER  - 

@article{
author = "Šegan, Sandra and Terzić-Jovanović, Nataša and Milojković-Opsenica, Dušanka and Trifković, Jelena and Šolaja, Bogdan and Opsenica, Dejan",
year = "2014",
abstract = "The chromatographic behavior of mixed 1,2,4,5-tetraoxanes, cholic and deoxycholic acid derivatives with distinct biological activity, was examined by high-performance thin-layer chromatography in order to correlate their structure and retention. Chromatographic systems were consisted of RP-18 or CN-silica as stationary phase, and binary mixtures of water with methanol, dioxane or acetone as mobile phase. Based on the respective retentions, the lipophilicity of the investigated compounds was determined. Multiple linear regression and partial least squares have been used to select variables that best describe the behavior of the investigated compounds in chromatographic systems and to quantify influences of most important parameters. The validation and cross-validation of the QSRR model suggest its applicability for prediction and understanding of retention of congeners. The models indicate the importance of nonpolar properties of the solutes and their ability for hydrophobic interactions, as well as the importance of proton donating abilities, hydrophilic and,pi interactions pointing out on that way the possible separation mechanism in the studied chromatographic systems. Observed correlations between structure and biological activity of mixed 1,2,4,5-tetraoxanes, indicate that the antimalarial activity against W2 and D6 Plasmodium falciparum strains, is governed by hydrophobic feature (measured with lipophilicity parameter), hydrophilic feature (measured with HLB, %HS, HB and HBA descriptors), and electronic feature (HOMO).",
publisher = "Elsevier",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "Correlation study of retention data and antimalarial activity of 1,2,4,5-mixed tetraoxanes with their molecular structure descriptors and LSER parameters",
volume = "97",
pages = "178-183",
doi = "10.1016/j.jpba.2014.04.029"
}

Šegan, S., Terzić-Jovanović, N., Milojković-Opsenica, D., Trifković, J., Šolaja, B.,& Opsenica, D.. (2014). Correlation study of retention data and antimalarial activity of 1,2,4,5-mixed tetraoxanes with their molecular structure descriptors and LSER parameters. in Journal of Pharmaceutical and Biomedical Analysis
Elsevier., 97, 178-183.
https://doi.org/10.1016/j.jpba.2014.04.029

Šegan S, Terzić-Jovanović N, Milojković-Opsenica D, Trifković J, Šolaja B, Opsenica D. Correlation study of retention data and antimalarial activity of 1,2,4,5-mixed tetraoxanes with their molecular structure descriptors and LSER parameters. in Journal of Pharmaceutical and Biomedical Analysis. 2014;97:178-183.
doi:10.1016/j.jpba.2014.04.029 .

Šegan, Sandra, Terzić-Jovanović, Nataša, Milojković-Opsenica, Dušanka, Trifković, Jelena, Šolaja, Bogdan, Opsenica, Dejan, "Correlation study of retention data and antimalarial activity of 1,2,4,5-mixed tetraoxanes with their molecular structure descriptors and LSER parameters" in Journal of Pharmaceutical and Biomedical Analysis, 97 (2014):178-183,
https://doi.org/10.1016/j.jpba.2014.04.029 . .

TY  - JOUR
AU  - Shweshein, Khalil Salem A. M.
AU  - Andrić, Filip
AU  - Radoičić, Aleksandra D.
AU  - Zlatar, Matija
AU  - Gruden-Pavlović, Maja
AU  - Tešić, Živoslav
AU  - Milojković-Opsenica, Dušanka
PY  - 2014
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1538
AB  - The lipophilicity of ten ruthenium(II)-arene complexes was assessed by reversed-phase thin-layer chromatography (RP-TLC) on octadecyl silica stationary phase. The binary solvent systems composed of water and acetonitrile were used as mobile phase in order to determine chromatographic descriptors for lipophilicity estimation. Octanol-water partition coefficient, logK(OW), of tested complexes was experimentally determined using twenty-eight standard solutes which were analyzed under the same chromatographic conditions as target substances. In addition, ab initio density functional theory (DFT) computational approach was employed to calculate logK(OW) values from the differences in Gibbs' free solvation energies of the solute transfer from n-octanol to water. A good overall agreement between DFT calculated and experimentally determined logK(OW) values was established (R-2 = 0.8024-0.9658).
PB  - Hindawi Publishing Corporation, New York
T2  - Scientific World Journal
T1  - Lipophilicity Assessment of Ruthenium(II)-Arene Complexes by the Means of Reversed-Phase Thin-Layer Chromatography and DFT Calculations
DO  - 10.1155/2014/862796
ER  - 

@article{
author = "Shweshein, Khalil Salem A. M. and Andrić, Filip and Radoičić, Aleksandra D. and Zlatar, Matija and Gruden-Pavlović, Maja and Tešić, Živoslav and Milojković-Opsenica, Dušanka",
year = "2014",
abstract = "The lipophilicity of ten ruthenium(II)-arene complexes was assessed by reversed-phase thin-layer chromatography (RP-TLC) on octadecyl silica stationary phase. The binary solvent systems composed of water and acetonitrile were used as mobile phase in order to determine chromatographic descriptors for lipophilicity estimation. Octanol-water partition coefficient, logK(OW), of tested complexes was experimentally determined using twenty-eight standard solutes which were analyzed under the same chromatographic conditions as target substances. In addition, ab initio density functional theory (DFT) computational approach was employed to calculate logK(OW) values from the differences in Gibbs' free solvation energies of the solute transfer from n-octanol to water. A good overall agreement between DFT calculated and experimentally determined logK(OW) values was established (R-2 = 0.8024-0.9658).",
publisher = "Hindawi Publishing Corporation, New York",
journal = "Scientific World Journal",
title = "Lipophilicity Assessment of Ruthenium(II)-Arene Complexes by the Means of Reversed-Phase Thin-Layer Chromatography and DFT Calculations",
doi = "10.1155/2014/862796"
}

Shweshein, K. S. A. M., Andrić, F., Radoičić, A. D., Zlatar, M., Gruden-Pavlović, M., Tešić, Ž.,& Milojković-Opsenica, D.. (2014). Lipophilicity Assessment of Ruthenium(II)-Arene Complexes by the Means of Reversed-Phase Thin-Layer Chromatography and DFT Calculations. in Scientific World Journal
Hindawi Publishing Corporation, New York..
https://doi.org/10.1155/2014/862796

Shweshein KSAM, Andrić F, Radoičić AD, Zlatar M, Gruden-Pavlović M, Tešić Ž, Milojković-Opsenica D. Lipophilicity Assessment of Ruthenium(II)-Arene Complexes by the Means of Reversed-Phase Thin-Layer Chromatography and DFT Calculations. in Scientific World Journal. 2014;.
doi:10.1155/2014/862796 .

Shweshein, Khalil Salem A. M., Andrić, Filip, Radoičić, Aleksandra D., Zlatar, Matija, Gruden-Pavlović, Maja, Tešić, Živoslav, Milojković-Opsenica, Dušanka, "Lipophilicity Assessment of Ruthenium(II)-Arene Complexes by the Means of Reversed-Phase Thin-Layer Chromatography and DFT Calculations" in Scientific World Journal (2014),
https://doi.org/10.1155/2014/862796 . .