TY  - JOUR
AU  - Jadranin, Milka
AU  - Savić, Danica
AU  - Lupšić, Ema
AU  - Podolski-Renić, Ana
AU  - Pešić, Milica
AU  - Tešević, Vele
AU  - Milosavljević, Slobodan M.
AU  - Krstić, Gordana B.
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7540
AB  - Euphorbia seguieriana ssp. seguieriana Necker (ES) and Euphorbia cyparissias (EC) with a habitat in the Deliblato Sands were the subject of this examination. The latexes of these so far insufficiently investigated species of the Euphorbia genus are used in traditional medicine for the treatment of wounds and warts on the skin. To determine their chemical composition, non-targeted screening of the latexes’ chloroform extracts was performed using liquid chromatography coupled with quadrupole time-of-flight mass spectrometry employing an electrospray ionization source (LC-ESI QTOF MS). The analysis of the obtained results showed that the latexes of ES and EC represent rich sources of diterpenes, tentatively identified as jatrophanes, ingenanes, tiglianes, myrsinanes, premyrsinanes, and others. Examination of the anticancer activity of the ES and EC latex extracts showed that both extracts significantly inhibited the growth of the non-small cell lung carcinoma NCI-H460 and glioblastoma U87 cell lines as well as of their corresponding multi-drug resistant (MDR) cell lines, NCI-H460/R and U87-TxR. The obtained results also revealed that the ES and EC extracts inhibited the function of P-glycoprotein (P-gp) in MDR cancer cells, whose overexpression is one of the main mechanisms underlying MDR.
PB  - MDPI
T2  - Plants
T1  - LC-ESI QToF MS Non-Targeted Screening of Latex Extracts of Euphorbia seguieriana ssp. seguieriana Necker and Euphorbia cyparissias and Determination of Their Potential Anticancer Activity
VL  - 12
IS  - 24
SP  - 4181
DO  - 10.3390/plants12244181
ER  - 

@article{
author = "Jadranin, Milka and Savić, Danica and Lupšić, Ema and Podolski-Renić, Ana and Pešić, Milica and Tešević, Vele and Milosavljević, Slobodan M. and Krstić, Gordana B.",
year = "2023",
abstract = "Euphorbia seguieriana ssp. seguieriana Necker (ES) and Euphorbia cyparissias (EC) with a habitat in the Deliblato Sands were the subject of this examination. The latexes of these so far insufficiently investigated species of the Euphorbia genus are used in traditional medicine for the treatment of wounds and warts on the skin. To determine their chemical composition, non-targeted screening of the latexes’ chloroform extracts was performed using liquid chromatography coupled with quadrupole time-of-flight mass spectrometry employing an electrospray ionization source (LC-ESI QTOF MS). The analysis of the obtained results showed that the latexes of ES and EC represent rich sources of diterpenes, tentatively identified as jatrophanes, ingenanes, tiglianes, myrsinanes, premyrsinanes, and others. Examination of the anticancer activity of the ES and EC latex extracts showed that both extracts significantly inhibited the growth of the non-small cell lung carcinoma NCI-H460 and glioblastoma U87 cell lines as well as of their corresponding multi-drug resistant (MDR) cell lines, NCI-H460/R and U87-TxR. The obtained results also revealed that the ES and EC extracts inhibited the function of P-glycoprotein (P-gp) in MDR cancer cells, whose overexpression is one of the main mechanisms underlying MDR.",
publisher = "MDPI",
journal = "Plants",
title = "LC-ESI QToF MS Non-Targeted Screening of Latex Extracts of Euphorbia seguieriana ssp. seguieriana Necker and Euphorbia cyparissias and Determination of Their Potential Anticancer Activity",
volume = "12",
number = "24",
pages = "4181",
doi = "10.3390/plants12244181"
}

Jadranin, M., Savić, D., Lupšić, E., Podolski-Renić, A., Pešić, M., Tešević, V., Milosavljević, S. M.,& Krstić, G. B.. (2023). LC-ESI QToF MS Non-Targeted Screening of Latex Extracts of Euphorbia seguieriana ssp. seguieriana Necker and Euphorbia cyparissias and Determination of Their Potential Anticancer Activity. in Plants
MDPI., 12(24), 4181.
https://doi.org/10.3390/plants12244181

Jadranin M, Savić D, Lupšić E, Podolski-Renić A, Pešić M, Tešević V, Milosavljević SM, Krstić GB. LC-ESI QToF MS Non-Targeted Screening of Latex Extracts of Euphorbia seguieriana ssp. seguieriana Necker and Euphorbia cyparissias and Determination of Their Potential Anticancer Activity. in Plants. 2023;12(24):4181.
doi:10.3390/plants12244181 .

Jadranin, Milka, Savić, Danica, Lupšić, Ema, Podolski-Renić, Ana, Pešić, Milica, Tešević, Vele, Milosavljević, Slobodan M., Krstić, Gordana B., "LC-ESI QToF MS Non-Targeted Screening of Latex Extracts of Euphorbia seguieriana ssp. seguieriana Necker and Euphorbia cyparissias and Determination of Their Potential Anticancer Activity" in Plants, 12, no. 24 (2023):4181,
https://doi.org/10.3390/plants12244181 . .

TY  - CONF
AU  - Jadranin, Milka
AU  - Krstić, Gordana
AU  - Savić, Danica
AU  - Novaković, Miroslav
AU  - Lekić, Stefan
AU  - Tešević, Vele
AU  - Pešić, Milica
AU  - Podolski-Renić, Ana
AU  - Lupšić, Ema
AU  - Milosavljević, Slobodan
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7270
AB  - Euphorbia cyparissias L. (cypress spurge) is one of the many species of the genus Euphorbia. Like other plant species of the genus, E. cyparissias is a source of biologically active compounds [1, 2]. The aim of this research was to profile the chloroform-methanol latex extract and detect the type of secondary metabolites of Serbian E. cyparissias, collected in Deliblato Sand 
(Serbia) in May 2022, using liquid chromatography – electrospray ionisation mass spectrometry. Based on the obtained results, terpene derivatives have been found as the most abundant in the latex extract, mainly diterpenes (jatrophanes and ingenanes) and triterpenes. Also, the anticancer activ ity of the latex extract on the cell lines NCI-H460, NCI-H460/R, U87, U87/
TxR and MRC-5, as well as the ability to inhibit P-gp, were examined. Latex extract exhibited anticancer activity against all tested cell lines, and also 
proved to be a strong P-gp inhibitor. The obtained results are in accordance with the literature data because jatrophanes are well-known strong P-gp 
inhibitors [3]. These preliminary results indicate that this plant material is a rich source of biologically active compounds and detailed phytochemical research of E. cyparissias is further planned.
PB  - International Conference on Natural Products Utilization - ICNPU2023
C3  - Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria
T1  - Analysis of terpenoids from the latex of Euphorbia cyparissias by liquid chromatography-electrospray ionization mass spectrometry
SP  - 184
EP  - 185
UR  - https://hdl.handle.net/21.15107/rcub_cer_7270
ER  - 

@conference{
author = "Jadranin, Milka and Krstić, Gordana and Savić, Danica and Novaković, Miroslav and Lekić, Stefan and Tešević, Vele and Pešić, Milica and Podolski-Renić, Ana and Lupšić, Ema and Milosavljević, Slobodan",
year = "2023",
abstract = "Euphorbia cyparissias L. (cypress spurge) is one of the many species of the genus Euphorbia. Like other plant species of the genus, E. cyparissias is a source of biologically active compounds [1, 2]. The aim of this research was to profile the chloroform-methanol latex extract and detect the type of secondary metabolites of Serbian E. cyparissias, collected in Deliblato Sand 
(Serbia) in May 2022, using liquid chromatography – electrospray ionisation mass spectrometry. Based on the obtained results, terpene derivatives have been found as the most abundant in the latex extract, mainly diterpenes (jatrophanes and ingenanes) and triterpenes. Also, the anticancer activ ity of the latex extract on the cell lines NCI-H460, NCI-H460/R, U87, U87/
TxR and MRC-5, as well as the ability to inhibit P-gp, were examined. Latex extract exhibited anticancer activity against all tested cell lines, and also 
proved to be a strong P-gp inhibitor. The obtained results are in accordance with the literature data because jatrophanes are well-known strong P-gp 
inhibitors [3]. These preliminary results indicate that this plant material is a rich source of biologically active compounds and detailed phytochemical research of E. cyparissias is further planned.",
publisher = "International Conference on Natural Products Utilization - ICNPU2023",
journal = "Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria",
title = "Analysis of terpenoids from the latex of Euphorbia cyparissias by liquid chromatography-electrospray ionization mass spectrometry",
pages = "184-185",
url = "https://hdl.handle.net/21.15107/rcub_cer_7270"
}

Jadranin, M., Krstić, G., Savić, D., Novaković, M., Lekić, S., Tešević, V., Pešić, M., Podolski-Renić, A., Lupšić, E.,& Milosavljević, S.. (2023). Analysis of terpenoids from the latex of Euphorbia cyparissias by liquid chromatography-electrospray ionization mass spectrometry. in Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria
International Conference on Natural Products Utilization - ICNPU2023., 184-185.
https://hdl.handle.net/21.15107/rcub_cer_7270

Jadranin M, Krstić G, Savić D, Novaković M, Lekić S, Tešević V, Pešić M, Podolski-Renić A, Lupšić E, Milosavljević S. Analysis of terpenoids from the latex of Euphorbia cyparissias by liquid chromatography-electrospray ionization mass spectrometry. in Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria. 2023;:184-185.
https://hdl.handle.net/21.15107/rcub_cer_7270 .

Jadranin, Milka, Krstić, Gordana, Savić, Danica, Novaković, Miroslav, Lekić, Stefan, Tešević, Vele, Pešić, Milica, Podolski-Renić, Ana, Lupšić, Ema, Milosavljević, Slobodan, "Analysis of terpenoids from the latex of Euphorbia cyparissias by liquid chromatography-electrospray ionization mass spectrometry" in Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria (2023):184-185,
https://hdl.handle.net/21.15107/rcub_cer_7270 .

TY  - CONF
AU  - Krstić, Gordana
AU  - Jadranin, Milka
AU  - Savić, Danica
AU  - Novaković, Miroslav
AU  - Lekić, Stefan
AU  - Tešević, Vele
AU  - Milosavljević, Slobodan
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7268
AB  - The genus Euphorbia represents one of the largest and most diverse plant genera. Plant species of this genera are a rich source of biologically active compounds. Our previous investigation of E. palustris L. revealed an excellent anti-melanoma activity of 3β-benzoyloxy-13α-dodecanoyloxy-ingenol (1), and 3β,13α,17-tribenzoyloxy-ingenol (2), significantly better than ingenol-mebutate (Picato®), a commercial anti-melanoma agent approved by the U.S. Food and Drug Administration (FDA) and by the European Medicines Agency (EMA) for the topical treatment of actinic keratosis [1]. Hence, in this research, using liquid chromatography–electrospray ionization mass spectrometry, chloroform-methanolic extracts of two samples of E. palustris collected at different localities in Serbia were analysed in order to confirm the presence of 1.Based on the tentative analysis of the LC-HRESI-MS and the previous examination of this plant species, ingenanes 1 and 2 were confirmed in both extracts examined. Additionally, both extracts contained several metabolites that structurally corresponds to ingenol, with a decanoyl group in their structure. Based on these preliminary results, our goal in further investigation is to isolate other ingenane molecules and test their anti-melanoma activity.
PB  - International Conference on Natural Products Utilization - ICNPU2023
C3  - Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria
T1  - LC-HRESI-MS Technique as the best choice for rapid screening of secondary metabolites of Euphorbia palustris latex extract
SP  - 43
EP  - 44
UR  - https://hdl.handle.net/21.15107/rcub_cer_7268
ER  - 

@conference{
author = "Krstić, Gordana and Jadranin, Milka and Savić, Danica and Novaković, Miroslav and Lekić, Stefan and Tešević, Vele and Milosavljević, Slobodan",
year = "2023",
abstract = "The genus Euphorbia represents one of the largest and most diverse plant genera. Plant species of this genera are a rich source of biologically active compounds. Our previous investigation of E. palustris L. revealed an excellent anti-melanoma activity of 3β-benzoyloxy-13α-dodecanoyloxy-ingenol (1), and 3β,13α,17-tribenzoyloxy-ingenol (2), significantly better than ingenol-mebutate (Picato®), a commercial anti-melanoma agent approved by the U.S. Food and Drug Administration (FDA) and by the European Medicines Agency (EMA) for the topical treatment of actinic keratosis [1]. Hence, in this research, using liquid chromatography–electrospray ionization mass spectrometry, chloroform-methanolic extracts of two samples of E. palustris collected at different localities in Serbia were analysed in order to confirm the presence of 1.Based on the tentative analysis of the LC-HRESI-MS and the previous examination of this plant species, ingenanes 1 and 2 were confirmed in both extracts examined. Additionally, both extracts contained several metabolites that structurally corresponds to ingenol, with a decanoyl group in their structure. Based on these preliminary results, our goal in further investigation is to isolate other ingenane molecules and test their anti-melanoma activity.",
publisher = "International Conference on Natural Products Utilization - ICNPU2023",
journal = "Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria",
title = "LC-HRESI-MS Technique as the best choice for rapid screening of secondary metabolites of Euphorbia palustris latex extract",
pages = "43-44",
url = "https://hdl.handle.net/21.15107/rcub_cer_7268"
}

Krstić, G., Jadranin, M., Savić, D., Novaković, M., Lekić, S., Tešević, V.,& Milosavljević, S.. (2023). LC-HRESI-MS Technique as the best choice for rapid screening of secondary metabolites of Euphorbia palustris latex extract. in Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria
International Conference on Natural Products Utilization - ICNPU2023., 43-44.
https://hdl.handle.net/21.15107/rcub_cer_7268

Krstić G, Jadranin M, Savić D, Novaković M, Lekić S, Tešević V, Milosavljević S. LC-HRESI-MS Technique as the best choice for rapid screening of secondary metabolites of Euphorbia palustris latex extract. in Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria. 2023;:43-44.
https://hdl.handle.net/21.15107/rcub_cer_7268 .

Krstić, Gordana, Jadranin, Milka, Savić, Danica, Novaković, Miroslav, Lekić, Stefan, Tešević, Vele, Milosavljević, Slobodan, "LC-HRESI-MS Technique as the best choice for rapid screening of secondary metabolites of Euphorbia palustris latex extract" in Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria (2023):43-44,
https://hdl.handle.net/21.15107/rcub_cer_7268 .

TY  - CONF
AU  - Novaković, Miroslav
AU  - Jadranin, Milka
AU  - Lekić, Stefan
AU  - Savić, Danica
AU  - Krstić, Gordana
AU  - Tešević, Vele
AU  - Milosavljević, Slobodan
PY  - 2023
AB  - Bisbibenzyls represent chemical class of compounds containing two bibenzyl units mutually connected by an oxygen atom and/or directly by the “C-C” bonds. They are chemical markers of liverworts, the ancestors of higher vascular plants. Marchantin A from Marchantia polymorpha was the first discovered bisbibenzyl. More than 120 chemically characterized bisbibenzyls 
were reported up to 2020 with numerous biological activities [1]. The first report on bisbibenzyls in higher (vascular) plants was published in 2007, when 
Kosenkova et al. (2007) isolated riccardin C in Primula veris subsp. macrocalyx from Mt. Altay in Russia as the dominant compound in the extract of the whole plant [2]. In the repeated study of the same species, Kosenkova et al. (2009) confirmed their findings discovering an additional bisbibenzyl constituent, perrottetin E [3].
In this investigation from the CH2Cl2/CH3OH (1:1) extract of the air-dried, powdered roots nine isbibenzyls, five new, have been isolated from P. veris subsp. columnae and P. acaulis using dry column flash chromatography followed by semipreparative HPLC chromatography and identified on the basis of 1D and 2D NMR, IR, UV and HRESIMS data. This investigation widens the knowledge about the presence of bisbibenzyls in vascular plants since only two bisbibenzyls have been previously found in vascular plants, in Primula veris subsp. macrocalyx.
PB  - International Conference on Natural Products Utilization - ICNPU2023
C3  - Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria
T1  - Bisbibenzyls in Primula species
SP  - 47
EP  - 48
UR  - https://hdl.handle.net/21.15107/rcub_cer_7269
ER  - 

@conference{
author = "Novaković, Miroslav and Jadranin, Milka and Lekić, Stefan and Savić, Danica and Krstić, Gordana and Tešević, Vele and Milosavljević, Slobodan",
year = "2023",
abstract = "Bisbibenzyls represent chemical class of compounds containing two bibenzyl units mutually connected by an oxygen atom and/or directly by the “C-C” bonds. They are chemical markers of liverworts, the ancestors of higher vascular plants. Marchantin A from Marchantia polymorpha was the first discovered bisbibenzyl. More than 120 chemically characterized bisbibenzyls 
were reported up to 2020 with numerous biological activities [1]. The first report on bisbibenzyls in higher (vascular) plants was published in 2007, when 
Kosenkova et al. (2007) isolated riccardin C in Primula veris subsp. macrocalyx from Mt. Altay in Russia as the dominant compound in the extract of the whole plant [2]. In the repeated study of the same species, Kosenkova et al. (2009) confirmed their findings discovering an additional bisbibenzyl constituent, perrottetin E [3].
In this investigation from the CH2Cl2/CH3OH (1:1) extract of the air-dried, powdered roots nine isbibenzyls, five new, have been isolated from P. veris subsp. columnae and P. acaulis using dry column flash chromatography followed by semipreparative HPLC chromatography and identified on the basis of 1D and 2D NMR, IR, UV and HRESIMS data. This investigation widens the knowledge about the presence of bisbibenzyls in vascular plants since only two bisbibenzyls have been previously found in vascular plants, in Primula veris subsp. macrocalyx.",
publisher = "International Conference on Natural Products Utilization - ICNPU2023",
journal = "Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria",
title = "Bisbibenzyls in Primula species",
pages = "47-48",
url = "https://hdl.handle.net/21.15107/rcub_cer_7269"
}

Novaković, M., Jadranin, M., Lekić, S., Savić, D., Krstić, G., Tešević, V.,& Milosavljević, S.. (2023). Bisbibenzyls in Primula species. in Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria
International Conference on Natural Products Utilization - ICNPU2023., 47-48.
https://hdl.handle.net/21.15107/rcub_cer_7269

Novaković M, Jadranin M, Lekić S, Savić D, Krstić G, Tešević V, Milosavljević S. Bisbibenzyls in Primula species. in Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria. 2023;:47-48.
https://hdl.handle.net/21.15107/rcub_cer_7269 .

Novaković, Miroslav, Jadranin, Milka, Lekić, Stefan, Savić, Danica, Krstić, Gordana, Tešević, Vele, Milosavljević, Slobodan, "Bisbibenzyls in Primula species" in Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria (2023):47-48,
https://hdl.handle.net/21.15107/rcub_cer_7269 .

TY  - CONF
AU  - Lekić, Stefan
AU  - Novaković, Miroslav
AU  - Savić, Danica
AU  - Тrendafilova, Antoaneta
AU  - Ivanova, Viktoria
AU  - Krstić, Gordana
AU  - Tešević, Vele
AU  - Milosavljević, Slobodan
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7271
AB  - In this investigation nine known diarylheptanoids, namely platyphyllenone, hirsutenone, alnuside A and B, platyphylloside, oregonin, platyphyllonol-5-O-β-D-xylopyranoside, oregonoyl A and oregonoyl B have 
been isolated from the bark of A. incana using dry column flash chromatography followed by semipreparative HPLC chromatography. The structure elucidation has been performed using 1D and 2D NMR, as well as, IR and UV spectroscopy.
PB  - International Conference on Natural Products Utilization - ICNPU2023
C3  - Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria
T1  - Diarylheptanoids from gray alder
SP  - 201
EP  - 200
UR  - https://hdl.handle.net/21.15107/rcub_cer_7271
ER  - 

@conference{
author = "Lekić, Stefan and Novaković, Miroslav and Savić, Danica and Тrendafilova, Antoaneta and Ivanova, Viktoria and Krstić, Gordana and Tešević, Vele and Milosavljević, Slobodan",
year = "2023",
abstract = "In this investigation nine known diarylheptanoids, namely platyphyllenone, hirsutenone, alnuside A and B, platyphylloside, oregonin, platyphyllonol-5-O-β-D-xylopyranoside, oregonoyl A and oregonoyl B have 
been isolated from the bark of A. incana using dry column flash chromatography followed by semipreparative HPLC chromatography. The structure elucidation has been performed using 1D and 2D NMR, as well as, IR and UV spectroscopy.",
publisher = "International Conference on Natural Products Utilization - ICNPU2023",
journal = "Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria",
title = "Diarylheptanoids from gray alder",
pages = "201-200",
url = "https://hdl.handle.net/21.15107/rcub_cer_7271"
}

Lekić, S., Novaković, M., Savić, D., Тrendafilova, A., Ivanova, V., Krstić, G., Tešević, V.,& Milosavljević, S.. (2023). Diarylheptanoids from gray alder. in Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria
International Conference on Natural Products Utilization - ICNPU2023., 201-200.
https://hdl.handle.net/21.15107/rcub_cer_7271

Lekić S, Novaković M, Savić D, Тrendafilova A, Ivanova V, Krstić G, Tešević V, Milosavljević S. Diarylheptanoids from gray alder. in Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria. 2023;:201-200.
https://hdl.handle.net/21.15107/rcub_cer_7271 .

Lekić, Stefan, Novaković, Miroslav, Savić, Danica, Тrendafilova, Antoaneta, Ivanova, Viktoria, Krstić, Gordana, Tešević, Vele, Milosavljević, Slobodan, "Diarylheptanoids from gray alder" in Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria (2023):201-200,
https://hdl.handle.net/21.15107/rcub_cer_7271 .

TY  - CONF
AU  - Savić, Danica
AU  - Krstić, Gordana
AU  - Jadranin, Milka
AU  - Novaković, Miroslav
AU  - Lekić, Stefan
AU  - Tešević, Vele
AU  - Milosavljević, Slobodan
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7272
AB  - The diversity of plant species from the genus Euphorbia and wide variety of secondary metabolites they produce contributed to their successful use in the traditional medicine all over the world. Therapeutic applications have been attributed to different compounds such as diterpenes and triterpenes. Previous investigations revealed that Euphorbia diterpenes possess a variety of biological activities such as anti-inflammatory, antitumor and antiviral. Some of them showed that tigliane diterpenes, beside other potential pharmaceutical activities, inhibited wild-type HIV-1 and HIV-2 strains as well as drug resistant strains of HIV 1 and that they exhibited low cytotoxicity. Two tigliane diterpenes were 
isolated from the latex of E. lucida, using classical and instrumental chromatographic techniques. ESI-HRMS spectra showed that both isolated compounds possess the same molecular formula (C31H38O6). 1D NMR spectra revealed benzoate and isobutanoate ester groups in both molecules, Using HMBC spectra their position was determined: C-13 for isobutanoate and C-20 for benzoate. Exact configurations were discovered by NOE correlations. It was concluded that these two molecules differ in the way of the binding the five-membered and seven-membered rings, i.e., that these two molecules are C-4-epimers. Compound 1 (20-benzoyloxy-13α-isobutanoyloxy-4,12-dideoxyphorbol) was already described in the literature as a metabolite of E. pannonica [1], while compound 2 (20-benzoyloxy-13α-isobutanoyloxy-4-epi-4,12-dideoxyphorbol) represents a new tigliane derivative.
PB  - International Conference on Natural Products Utilization - ICNPU2023
C3  - Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria
T1  - Tigliane diterpenes isolated from the latex of Euphorbia lucida
SP  - 239
EP  - 239
UR  - https://hdl.handle.net/21.15107/rcub_cer_7272
ER  - 

@conference{
author = "Savić, Danica and Krstić, Gordana and Jadranin, Milka and Novaković, Miroslav and Lekić, Stefan and Tešević, Vele and Milosavljević, Slobodan",
year = "2023",
abstract = "The diversity of plant species from the genus Euphorbia and wide variety of secondary metabolites they produce contributed to their successful use in the traditional medicine all over the world. Therapeutic applications have been attributed to different compounds such as diterpenes and triterpenes. Previous investigations revealed that Euphorbia diterpenes possess a variety of biological activities such as anti-inflammatory, antitumor and antiviral. Some of them showed that tigliane diterpenes, beside other potential pharmaceutical activities, inhibited wild-type HIV-1 and HIV-2 strains as well as drug resistant strains of HIV 1 and that they exhibited low cytotoxicity. Two tigliane diterpenes were 
isolated from the latex of E. lucida, using classical and instrumental chromatographic techniques. ESI-HRMS spectra showed that both isolated compounds possess the same molecular formula (C31H38O6). 1D NMR spectra revealed benzoate and isobutanoate ester groups in both molecules, Using HMBC spectra their position was determined: C-13 for isobutanoate and C-20 for benzoate. Exact configurations were discovered by NOE correlations. It was concluded that these two molecules differ in the way of the binding the five-membered and seven-membered rings, i.e., that these two molecules are C-4-epimers. Compound 1 (20-benzoyloxy-13α-isobutanoyloxy-4,12-dideoxyphorbol) was already described in the literature as a metabolite of E. pannonica [1], while compound 2 (20-benzoyloxy-13α-isobutanoyloxy-4-epi-4,12-dideoxyphorbol) represents a new tigliane derivative.",
publisher = "International Conference on Natural Products Utilization - ICNPU2023",
journal = "Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria",
title = "Tigliane diterpenes isolated from the latex of Euphorbia lucida",
pages = "239-239",
url = "https://hdl.handle.net/21.15107/rcub_cer_7272"
}

Savić, D., Krstić, G., Jadranin, M., Novaković, M., Lekić, S., Tešević, V.,& Milosavljević, S.. (2023). Tigliane diterpenes isolated from the latex of Euphorbia lucida. in Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria
International Conference on Natural Products Utilization - ICNPU2023., 239-239.
https://hdl.handle.net/21.15107/rcub_cer_7272

Savić D, Krstić G, Jadranin M, Novaković M, Lekić S, Tešević V, Milosavljević S. Tigliane diterpenes isolated from the latex of Euphorbia lucida. in Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria. 2023;:239-239.
https://hdl.handle.net/21.15107/rcub_cer_7272 .

Savić, Danica, Krstić, Gordana, Jadranin, Milka, Novaković, Miroslav, Lekić, Stefan, Tešević, Vele, Milosavljević, Slobodan, "Tigliane diterpenes isolated from the latex of Euphorbia lucida" in Book of Abstracts - The 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf (ICNPU-2023), 30 May–02 June 2023, Sts. Constantine and Helena - Varna, Bulgaria (2023):239-239,
https://hdl.handle.net/21.15107/rcub_cer_7272 .

TY  - CONF
AU  - Savić, Danica
AU  - Krstić, Gordana
AU  - Jadranin, Milka
AU  - Tešević, Vele
AU  - Milosavljević, Slobodan
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5875
AB  - Plant species of the genus Euphorbia have an important place in the traditional medicine due to the secondary metabolites which they produce. The most important metabolites  come from the classes of diterpenes and triterpenes. The studies have shown that these  compounds can have antitumour, antiviral, antibacterial, anti-inflammatory and other  properties. The aim of the research was to investigatethe triterpene composition of  Euphorbia palustris. Using chromatographic techniques, four triterpene derivatives were  isolated from the plant material, as follows: (+)-tirucallol (1), lanosterol (2), 27-nor-3βhydroxycycloart-23-en-25-one (3) and (+)-obtusifoliol (4). In the continuation of the  research, their biological activities will be examined.
AB  - Biljne vrste roda Euphorbia imaju važno mesto u tradicionalnoj medicini zahvaljujući  sekundarnim metabolitima koje proizvode. Najznačajniji metaboliti pripadaju klasama  diterpena i triterpena. Istraživanja su pokazala da ova jedinjenja imaju antitumorska,  antivirusna, antibakterijska, antiinflamatorna i druga svojstva. Cilj ovog istraživanja bio je  ispitivanje triterpenskog sastava biljne vrste Euphorbia palustris. Primenom  hromatografskih tehnika iz biljnog materijala izolovana su četiri triterpenska derivata i  to:(+)-tirukalol (1), lanosterol (2), 27-nor-3β-hidroksicikloart-23-en-25-on (3) i (+)- obtusifoliol (4). U nastavku istraživanja biće ispitane njihove biološke aktivnosti.
PB  - Serbian Chemical Society
C3  - Book of Abstracts, Proceedings - 58th Meeting of the Serbian Chemical Society, Belgrade, Serbia, June 9-10, 2022 / Kratki izvodi radova, kjniga radova - 58. Savetovanje Srpskog hemijskog društva, Beograd 9. i 10. jun 2022. godine
T1  - Triterpene composition of the plant species Euphorbia palustris
T1  - Triterpeni biljne vrste Euphorbia palustris
SP  - 143
UR  - https://hdl.handle.net/21.15107/rcub_cer_5875
ER  - 

@conference{
author = "Savić, Danica and Krstić, Gordana and Jadranin, Milka and Tešević, Vele and Milosavljević, Slobodan",
year = "2022",
abstract = "Plant species of the genus Euphorbia have an important place in the traditional medicine due to the secondary metabolites which they produce. The most important metabolites  come from the classes of diterpenes and triterpenes. The studies have shown that these  compounds can have antitumour, antiviral, antibacterial, anti-inflammatory and other  properties. The aim of the research was to investigatethe triterpene composition of  Euphorbia palustris. Using chromatographic techniques, four triterpene derivatives were  isolated from the plant material, as follows: (+)-tirucallol (1), lanosterol (2), 27-nor-3βhydroxycycloart-23-en-25-one (3) and (+)-obtusifoliol (4). In the continuation of the  research, their biological activities will be examined., Biljne vrste roda Euphorbia imaju važno mesto u tradicionalnoj medicini zahvaljujući  sekundarnim metabolitima koje proizvode. Najznačajniji metaboliti pripadaju klasama  diterpena i triterpena. Istraživanja su pokazala da ova jedinjenja imaju antitumorska,  antivirusna, antibakterijska, antiinflamatorna i druga svojstva. Cilj ovog istraživanja bio je  ispitivanje triterpenskog sastava biljne vrste Euphorbia palustris. Primenom  hromatografskih tehnika iz biljnog materijala izolovana su četiri triterpenska derivata i  to:(+)-tirukalol (1), lanosterol (2), 27-nor-3β-hidroksicikloart-23-en-25-on (3) i (+)- obtusifoliol (4). U nastavku istraživanja biće ispitane njihove biološke aktivnosti.",
publisher = "Serbian Chemical Society",
journal = "Book of Abstracts, Proceedings - 58th Meeting of the Serbian Chemical Society, Belgrade, Serbia, June 9-10, 2022 / Kratki izvodi radova, kjniga radova - 58. Savetovanje Srpskog hemijskog društva, Beograd 9. i 10. jun 2022. godine",
title = "Triterpene composition of the plant species Euphorbia palustris, Triterpeni biljne vrste Euphorbia palustris",
pages = "143",
url = "https://hdl.handle.net/21.15107/rcub_cer_5875"
}

Savić, D., Krstić, G., Jadranin, M., Tešević, V.,& Milosavljević, S.. (2022). Triterpene composition of the plant species Euphorbia palustris. in Book of Abstracts, Proceedings - 58th Meeting of the Serbian Chemical Society, Belgrade, Serbia, June 9-10, 2022 / Kratki izvodi radova, kjniga radova - 58. Savetovanje Srpskog hemijskog društva, Beograd 9. i 10. jun 2022. godine
Serbian Chemical Society., 143.
https://hdl.handle.net/21.15107/rcub_cer_5875

Savić D, Krstić G, Jadranin M, Tešević V, Milosavljević S. Triterpene composition of the plant species Euphorbia palustris. in Book of Abstracts, Proceedings - 58th Meeting of the Serbian Chemical Society, Belgrade, Serbia, June 9-10, 2022 / Kratki izvodi radova, kjniga radova - 58. Savetovanje Srpskog hemijskog društva, Beograd 9. i 10. jun 2022. godine. 2022;:143.
https://hdl.handle.net/21.15107/rcub_cer_5875 .

Savić, Danica, Krstić, Gordana, Jadranin, Milka, Tešević, Vele, Milosavljević, Slobodan, "Triterpene composition of the plant species Euphorbia palustris" in Book of Abstracts, Proceedings - 58th Meeting of the Serbian Chemical Society, Belgrade, Serbia, June 9-10, 2022 / Kratki izvodi radova, kjniga radova - 58. Savetovanje Srpskog hemijskog društva, Beograd 9. i 10. jun 2022. godine (2022):143,
https://hdl.handle.net/21.15107/rcub_cer_5875 .