TY  - JOUR
AU  - Nicoleti, Jorge Luis
AU  - Braga, Erik Sobrinho
AU  - Stanisic, Danijela
AU  - Jadranin, Milka
AU  - Façanha, Débora Andréa Evangelista
AU  - Barral, Thiago Doria
AU  - Hanna, Samira Abdallah
AU  - Azevedo, Vasco
AU  - Meyer, Roberto
AU  - Tasic, Ljubica
AU  - Portela, Ricardo Wagner
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7094
AB  - Caseous lymphadenitis (CLA), an infectious disease caused by Corynebacterium pseudotuberculosis in small ruminants, is highly prevalent worldwide. Economic losses have already been associated with the disease, and little is known about the host–pathogen relationship associated with the disease. The present study aimed to perform a metabolomic study of the C. pseudotuberculosis infection in goats. Serum samples were collected from a herd of 173 goats. The animals were classified as controls (not infected), asymptomatic (seropositives but without detectable CLA clinical signs), and symptomatic (seropositive animals presenting CLA lesions), according to microbiological isolation and immunodiagnosis. The serum samples were analyzed using nuclear magnetic resonance (1H-NMR), nuclear Overhauser effect spectroscopy (NOESY), and Carr–Purcell–Meiboom–Gill (CPMG) sequences. The NMR data were analyzed using chemometrics, and principal component analysis (PCA) and partial least square discriminant analysis (PLS-DA) were performed to discover specific biomarkers responsible for discrimination between the groups. A high dissemination of the infection by C. pseudotuberculosis was observed, being 74.57% asymptomatic and 11.56% symptomatic. In the evaluation of 62 serum samples by NMR, the techniques were satisfactory in the discrimination of the groups, being also complementary and mutually confirming, demonstrating possible biomarkers for the infection by the bacterium. Twenty metabolites of interest were identified by NOESY and 29 by CPMG, such as tryptophan, polyunsaturated fatty acids, formic acid, NAD+, and 3-hydroxybutyrate, opening promising possibilities for the use of these results in new therapeutic, immunodiagnosis, and immunoprophylactic tools, as well as for studies of the immune response against C. pseudotuberculosis.
PB  - Springer Science and Business Media LLC
T2  - Applied Microbiology and Biotechnology
T1  - A serum NMR metabolomic analysis of the Corynebacterium pseudotuberculosis infection in goats
VL  - 107
IS  - 14
SP  - 4593
EP  - 4603
DO  - 10.1007/s00253-023-12595-0
ER  - 

@article{
author = "Nicoleti, Jorge Luis and Braga, Erik Sobrinho and Stanisic, Danijela and Jadranin, Milka and Façanha, Débora Andréa Evangelista and Barral, Thiago Doria and Hanna, Samira Abdallah and Azevedo, Vasco and Meyer, Roberto and Tasic, Ljubica and Portela, Ricardo Wagner",
year = "2023",
abstract = "Caseous lymphadenitis (CLA), an infectious disease caused by Corynebacterium pseudotuberculosis in small ruminants, is highly prevalent worldwide. Economic losses have already been associated with the disease, and little is known about the host–pathogen relationship associated with the disease. The present study aimed to perform a metabolomic study of the C. pseudotuberculosis infection in goats. Serum samples were collected from a herd of 173 goats. The animals were classified as controls (not infected), asymptomatic (seropositives but without detectable CLA clinical signs), and symptomatic (seropositive animals presenting CLA lesions), according to microbiological isolation and immunodiagnosis. The serum samples were analyzed using nuclear magnetic resonance (1H-NMR), nuclear Overhauser effect spectroscopy (NOESY), and Carr–Purcell–Meiboom–Gill (CPMG) sequences. The NMR data were analyzed using chemometrics, and principal component analysis (PCA) and partial least square discriminant analysis (PLS-DA) were performed to discover specific biomarkers responsible for discrimination between the groups. A high dissemination of the infection by C. pseudotuberculosis was observed, being 74.57% asymptomatic and 11.56% symptomatic. In the evaluation of 62 serum samples by NMR, the techniques were satisfactory in the discrimination of the groups, being also complementary and mutually confirming, demonstrating possible biomarkers for the infection by the bacterium. Twenty metabolites of interest were identified by NOESY and 29 by CPMG, such as tryptophan, polyunsaturated fatty acids, formic acid, NAD+, and 3-hydroxybutyrate, opening promising possibilities for the use of these results in new therapeutic, immunodiagnosis, and immunoprophylactic tools, as well as for studies of the immune response against C. pseudotuberculosis.",
publisher = "Springer Science and Business Media LLC",
journal = "Applied Microbiology and Biotechnology",
title = "A serum NMR metabolomic analysis of the Corynebacterium pseudotuberculosis infection in goats",
volume = "107",
number = "14",
pages = "4593-4603",
doi = "10.1007/s00253-023-12595-0"
}

Nicoleti, J. L., Braga, E. S., Stanisic, D., Jadranin, M., Façanha, D. A. E., Barral, T. D., Hanna, S. A., Azevedo, V., Meyer, R., Tasic, L.,& Portela, R. W.. (2023). A serum NMR metabolomic analysis of the Corynebacterium pseudotuberculosis infection in goats. in Applied Microbiology and Biotechnology
Springer Science and Business Media LLC., 107(14), 4593-4603.
https://doi.org/10.1007/s00253-023-12595-0

Nicoleti JL, Braga ES, Stanisic D, Jadranin M, Façanha DAE, Barral TD, Hanna SA, Azevedo V, Meyer R, Tasic L, Portela RW. A serum NMR metabolomic analysis of the Corynebacterium pseudotuberculosis infection in goats. in Applied Microbiology and Biotechnology. 2023;107(14):4593-4603.
doi:10.1007/s00253-023-12595-0 .

Nicoleti, Jorge Luis, Braga, Erik Sobrinho, Stanisic, Danijela, Jadranin, Milka, Façanha, Débora Andréa Evangelista, Barral, Thiago Doria, Hanna, Samira Abdallah, Azevedo, Vasco, Meyer, Roberto, Tasic, Ljubica, Portela, Ricardo Wagner, "A serum NMR metabolomic analysis of the Corynebacterium pseudotuberculosis infection in goats" in Applied Microbiology and Biotechnology, 107, no. 14 (2023):4593-4603,
https://doi.org/10.1007/s00253-023-12595-0 . .

TY  - JOUR
AU  - Tasic, Ljubica
AU  - Avramović, Nataša
AU  - Jadranin, Milka
AU  - Quintero, Melissa
AU  - Stanisic, Danijela
AU  - Martins, Lucas G.
AU  - Costa, Tássia Brena Barroso Carneiro
AU  - Novak, Estela
AU  - Odone, Vicente
AU  - Rivas, Maria
AU  - Aguiar, Talita
AU  - Carraro, Dirce Maria
AU  - Werneck da Cunha, Isabela
AU  - Lima da Costa, Cecilia Maria
AU  - Maschietto, Mariana
AU  - Krepischi, Ana
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5568
AB  - Cancer is one of the leading causes of death in children and adolescents worldwide; among the types of liver cancer, hepatoblastoma (HBL) is the most common in childhood. Although it affects only two to three individuals in a million, it is mostly asymptomatic at diagnosis, so by the time it is detected it has already advanced. There are specific recommendations regarding HBL treatment, and ongoing studies to stratify the risks of HBL, understand the pathology, and predict prognostics and survival rates. Although magnetic resonance imaging spectroscopy is frequently used in diagnostics of HBL, high-resolution magic-angle-spinning (HR-MAS) NMR spectroscopy of HBL tissues is scarce. Using this technique, we studied the alterations among tissue metabolites of ex vivo samples from (a) HBL and non-cancer liver tissues (NCL), (b) HBL and adjacent non-tumor samples, and (c) two regions of the same HBL samples, one more centralized and the other at the edge of the tumor. It was possible to identify metabolites in HBL, then metabolites from the HBL center and the border samples, and link them to altered metabolisms in tumor tissues, highlighting their potential as biochemical markers. Metabolites closely related to liver metabolisms such as some phospholipids, triacylglycerides, fatty acids, glucose, and amino acids showed differences between the tissues.
PB  - MDPI AG
T2  - Biomedicines
T1  - High-Resolution Magic-Angle-Spinning NMR in Revealing Hepatoblastoma Hallmarks
VL  - 10
IS  - 12
SP  - 3091
DO  - 10.3390/biomedicines10123091
ER  - 

@article{
author = "Tasic, Ljubica and Avramović, Nataša and Jadranin, Milka and Quintero, Melissa and Stanisic, Danijela and Martins, Lucas G. and Costa, Tássia Brena Barroso Carneiro and Novak, Estela and Odone, Vicente and Rivas, Maria and Aguiar, Talita and Carraro, Dirce Maria and Werneck da Cunha, Isabela and Lima da Costa, Cecilia Maria and Maschietto, Mariana and Krepischi, Ana",
year = "2022",
abstract = "Cancer is one of the leading causes of death in children and adolescents worldwide; among the types of liver cancer, hepatoblastoma (HBL) is the most common in childhood. Although it affects only two to three individuals in a million, it is mostly asymptomatic at diagnosis, so by the time it is detected it has already advanced. There are specific recommendations regarding HBL treatment, and ongoing studies to stratify the risks of HBL, understand the pathology, and predict prognostics and survival rates. Although magnetic resonance imaging spectroscopy is frequently used in diagnostics of HBL, high-resolution magic-angle-spinning (HR-MAS) NMR spectroscopy of HBL tissues is scarce. Using this technique, we studied the alterations among tissue metabolites of ex vivo samples from (a) HBL and non-cancer liver tissues (NCL), (b) HBL and adjacent non-tumor samples, and (c) two regions of the same HBL samples, one more centralized and the other at the edge of the tumor. It was possible to identify metabolites in HBL, then metabolites from the HBL center and the border samples, and link them to altered metabolisms in tumor tissues, highlighting their potential as biochemical markers. Metabolites closely related to liver metabolisms such as some phospholipids, triacylglycerides, fatty acids, glucose, and amino acids showed differences between the tissues.",
publisher = "MDPI AG",
journal = "Biomedicines",
title = "High-Resolution Magic-Angle-Spinning NMR in Revealing Hepatoblastoma Hallmarks",
volume = "10",
number = "12",
pages = "3091",
doi = "10.3390/biomedicines10123091"
}

Tasic, L., Avramović, N., Jadranin, M., Quintero, M., Stanisic, D., Martins, L. G., Costa, T. B. B. C., Novak, E., Odone, V., Rivas, M., Aguiar, T., Carraro, D. M., Werneck da Cunha, I., Lima da Costa, C. M., Maschietto, M.,& Krepischi, A.. (2022). High-Resolution Magic-Angle-Spinning NMR in Revealing Hepatoblastoma Hallmarks. in Biomedicines
MDPI AG., 10(12), 3091.
https://doi.org/10.3390/biomedicines10123091

Tasic L, Avramović N, Jadranin M, Quintero M, Stanisic D, Martins LG, Costa TBBC, Novak E, Odone V, Rivas M, Aguiar T, Carraro DM, Werneck da Cunha I, Lima da Costa CM, Maschietto M, Krepischi A. High-Resolution Magic-Angle-Spinning NMR in Revealing Hepatoblastoma Hallmarks. in Biomedicines. 2022;10(12):3091.
doi:10.3390/biomedicines10123091 .

Tasic, Ljubica, Avramović, Nataša, Jadranin, Milka, Quintero, Melissa, Stanisic, Danijela, Martins, Lucas G., Costa, Tássia Brena Barroso Carneiro, Novak, Estela, Odone, Vicente, Rivas, Maria, Aguiar, Talita, Carraro, Dirce Maria, Werneck da Cunha, Isabela, Lima da Costa, Cecilia Maria, Maschietto, Mariana, Krepischi, Ana, "High-Resolution Magic-Angle-Spinning NMR in Revealing Hepatoblastoma Hallmarks" in Biomedicines, 10, no. 12 (2022):3091,
https://doi.org/10.3390/biomedicines10123091 . .