TY  - JOUR
AU  - Mijin, Nemanja
AU  - Milošević, Jelica
AU  - Stevanović, Sanja
AU  - Petrović, Predrag
AU  - Lolić, Aleksandar
AU  - Urbic, Tomaz
AU  - Polović, Natalija
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7189
AB  - The aggregation of proteins into fibrillar, amyloid-like aggregates generally results in an improved, positive effect on various techno-functional properties within food products, such as gelation, emulsification, and foam stabilization. These highly stable structures, characterized by their repetitive, β-sheet rich motifs, may develop as the result of the thermal treatment of protein-rich food products. Heavy metal ions can influence amyloid-like aggregation of food proteins. Lead(II) and cadmium(II) represent some of the most abundant and common environmental water and food pollutants. In this work, the influence of heavy metal ions, lead and cadmium on amyloid-like aggregation of ovalbumin at high temperatures (90 °C) and under acidic conditions (pH 2.0) was investigated. Ovalbumin is used as a general model for how heavy metals can affect amyloid-like aggregation of a food protein. Structural changes were monitored via Thioflavin T and 8-Anilino-1-naphthalenesulfonic acid fluorescence, Fourier-Transform infrared spectroscopy, atomic force microscopy, dynamic light scattering, as well as computational analyses. The obtained results indicate that the added heavy metal ions bind to different sites within ovalbumin prior to thermal treatment. Lead binding sites are closer to the hydrophobic regions of an protein, while cadmium ion binding sites are more exposed. This specific binding of metal ions affects the morphologies of amyloid-like aggregates, resulting in lead-induced branching of amyloid-like fibrils, or cadmium-induced tangling of fibrils into dense amyloid clusters. This additive effect of heavy metal ions is most evident in ovalbumin samples which contain a mixture of both heavy metal ions.
PB  - Elsevier B.V.
T2  - Food Hydrocolloids
T1  - Amyloid-like aggregation influenced by lead(II) and cadmium(II) ions in hen egg white ovalbumin
VL  - 136
SP  - 108292
DO  - 10.1016/j.foodhyd.2022.108292
ER  - 

@article{
author = "Mijin, Nemanja and Milošević, Jelica and Stevanović, Sanja and Petrović, Predrag and Lolić, Aleksandar and Urbic, Tomaz and Polović, Natalija",
year = "2023",
abstract = "The aggregation of proteins into fibrillar, amyloid-like aggregates generally results in an improved, positive effect on various techno-functional properties within food products, such as gelation, emulsification, and foam stabilization. These highly stable structures, characterized by their repetitive, β-sheet rich motifs, may develop as the result of the thermal treatment of protein-rich food products. Heavy metal ions can influence amyloid-like aggregation of food proteins. Lead(II) and cadmium(II) represent some of the most abundant and common environmental water and food pollutants. In this work, the influence of heavy metal ions, lead and cadmium on amyloid-like aggregation of ovalbumin at high temperatures (90 °C) and under acidic conditions (pH 2.0) was investigated. Ovalbumin is used as a general model for how heavy metals can affect amyloid-like aggregation of a food protein. Structural changes were monitored via Thioflavin T and 8-Anilino-1-naphthalenesulfonic acid fluorescence, Fourier-Transform infrared spectroscopy, atomic force microscopy, dynamic light scattering, as well as computational analyses. The obtained results indicate that the added heavy metal ions bind to different sites within ovalbumin prior to thermal treatment. Lead binding sites are closer to the hydrophobic regions of an protein, while cadmium ion binding sites are more exposed. This specific binding of metal ions affects the morphologies of amyloid-like aggregates, resulting in lead-induced branching of amyloid-like fibrils, or cadmium-induced tangling of fibrils into dense amyloid clusters. This additive effect of heavy metal ions is most evident in ovalbumin samples which contain a mixture of both heavy metal ions.",
publisher = "Elsevier B.V.",
journal = "Food Hydrocolloids",
title = "Amyloid-like aggregation influenced by lead(II) and cadmium(II) ions in hen egg white ovalbumin",
volume = "136",
pages = "108292",
doi = "10.1016/j.foodhyd.2022.108292"
}

Mijin, N., Milošević, J., Stevanović, S., Petrović, P., Lolić, A., Urbic, T.,& Polović, N.. (2023). Amyloid-like aggregation influenced by lead(II) and cadmium(II) ions in hen egg white ovalbumin. in Food Hydrocolloids
Elsevier B.V.., 136, 108292.
https://doi.org/10.1016/j.foodhyd.2022.108292

Mijin N, Milošević J, Stevanović S, Petrović P, Lolić A, Urbic T, Polović N. Amyloid-like aggregation influenced by lead(II) and cadmium(II) ions in hen egg white ovalbumin. in Food Hydrocolloids. 2023;136:108292.
doi:10.1016/j.foodhyd.2022.108292 .

Mijin, Nemanja, Milošević, Jelica, Stevanović, Sanja, Petrović, Predrag, Lolić, Aleksandar, Urbic, Tomaz, Polović, Natalija, "Amyloid-like aggregation influenced by lead(II) and cadmium(II) ions in hen egg white ovalbumin" in Food Hydrocolloids, 136 (2023):108292,
https://doi.org/10.1016/j.foodhyd.2022.108292 . .

TY  - CONF
AU  - Milošević, Dragana
AU  - Milošević, Milena
AU  - Perendija, Jovana
AU  - Milovanović, Željka
AU  - Petrović, Predrag
AU  - Marinković, Aleksandar
AU  - Petrović, Rada
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6472
AB  - Water pollution is a consequence of permanent technological development, urbanization and industrialization, having a huge impact on human health and the environment. Thus, metal oxides received considerable scientific interest in the field of adsorption to reduce heavy metal contamination from water and wastewater. Three-dimensionally ordered macroporous (3DOM) alumina modified with iron oxide and (3-aminopropyl)triethoxysilane (APTES) ((FeAl)2O3-APTES) was synthesized and linked with subgleba of Handkea utriformis (S) through 3-(Carbomethoxy)propionyl chloride (CPC). Surface hydroxyl groups of metal oxides have an affinity for cations while the introduction of amino groups in (FeAl)2O3-APTES allows the creation of covalent attachment on subgleba. Subglebal tissue of mosaic puffball Handkea utriformis contains sugars, proteins and melanins i.e. a large number of functional groups that could interact with an adsorbate. The obtained adsorbent (SCPC-(FeAl)2O3-APTES) was used for Pb2+ removal from aqueous solutions under batch conditions, constant initial concentration of adsorbate and pH and different concentrations of the adsorbent. Composition and surface morphology were characterized by using FT-IR and  SEM analysis. Concentrations of Pb2+, before and after adsorption, were determined by using Atomic Absorption Spectroscopy (AAS). The nonlinear Langmuir and Freundlich isotherm models were evaluated to correlate experimental data in which best fit was achieved with the Langmuir isotherm model. The specific surface area (SBET) of the subgleba was very low; porosity and SBET were increased by (FeAl)2O3-APTES deposition onto SCPC, indicating the formation of porous metal oxides deposits. This modification contributed to a significant increase in adsorption capacity.
PB  - Faculty of Technology Leskovac
C3  - Book of Abstracts - 14th Symposium Novel Technologies and Economic Development, 22th-23th October 2021, Leskovac, Serbia
T1  - Structurally modified three-dimensionally ordered (3DOM) alumina linked to subgleba of Handkea utriformis for Pb2+ removal
SP  - 110
EP  - 110
UR  - https://hdl.handle.net/21.15107/rcub_cer_6472
ER  - 

@conference{
author = "Milošević, Dragana and Milošević, Milena and Perendija, Jovana and Milovanović, Željka and Petrović, Predrag and Marinković, Aleksandar and Petrović, Rada",
year = "2021",
abstract = "Water pollution is a consequence of permanent technological development, urbanization and industrialization, having a huge impact on human health and the environment. Thus, metal oxides received considerable scientific interest in the field of adsorption to reduce heavy metal contamination from water and wastewater. Three-dimensionally ordered macroporous (3DOM) alumina modified with iron oxide and (3-aminopropyl)triethoxysilane (APTES) ((FeAl)2O3-APTES) was synthesized and linked with subgleba of Handkea utriformis (S) through 3-(Carbomethoxy)propionyl chloride (CPC). Surface hydroxyl groups of metal oxides have an affinity for cations while the introduction of amino groups in (FeAl)2O3-APTES allows the creation of covalent attachment on subgleba. Subglebal tissue of mosaic puffball Handkea utriformis contains sugars, proteins and melanins i.e. a large number of functional groups that could interact with an adsorbate. The obtained adsorbent (SCPC-(FeAl)2O3-APTES) was used for Pb2+ removal from aqueous solutions under batch conditions, constant initial concentration of adsorbate and pH and different concentrations of the adsorbent. Composition and surface morphology were characterized by using FT-IR and  SEM analysis. Concentrations of Pb2+, before and after adsorption, were determined by using Atomic Absorption Spectroscopy (AAS). The nonlinear Langmuir and Freundlich isotherm models were evaluated to correlate experimental data in which best fit was achieved with the Langmuir isotherm model. The specific surface area (SBET) of the subgleba was very low; porosity and SBET were increased by (FeAl)2O3-APTES deposition onto SCPC, indicating the formation of porous metal oxides deposits. This modification contributed to a significant increase in adsorption capacity.",
publisher = "Faculty of Technology Leskovac",
journal = "Book of Abstracts - 14th Symposium Novel Technologies and Economic Development, 22th-23th October 2021, Leskovac, Serbia",
title = "Structurally modified three-dimensionally ordered (3DOM) alumina linked to subgleba of Handkea utriformis for Pb2+ removal",
pages = "110-110",
url = "https://hdl.handle.net/21.15107/rcub_cer_6472"
}

Milošević, D., Milošević, M., Perendija, J., Milovanović, Ž., Petrović, P., Marinković, A.,& Petrović, R.. (2021). Structurally modified three-dimensionally ordered (3DOM) alumina linked to subgleba of Handkea utriformis for Pb2+ removal. in Book of Abstracts - 14th Symposium Novel Technologies and Economic Development, 22th-23th October 2021, Leskovac, Serbia
Faculty of Technology Leskovac., 110-110.
https://hdl.handle.net/21.15107/rcub_cer_6472

Milošević D, Milošević M, Perendija J, Milovanović Ž, Petrović P, Marinković A, Petrović R. Structurally modified three-dimensionally ordered (3DOM) alumina linked to subgleba of Handkea utriformis for Pb2+ removal. in Book of Abstracts - 14th Symposium Novel Technologies and Economic Development, 22th-23th October 2021, Leskovac, Serbia. 2021;:110-110.
https://hdl.handle.net/21.15107/rcub_cer_6472 .

Milošević, Dragana, Milošević, Milena, Perendija, Jovana, Milovanović, Željka, Petrović, Predrag, Marinković, Aleksandar, Petrović, Rada, "Structurally modified three-dimensionally ordered (3DOM) alumina linked to subgleba of Handkea utriformis for Pb2+ removal" in Book of Abstracts - 14th Symposium Novel Technologies and Economic Development, 22th-23th October 2021, Leskovac, Serbia (2021):110-110,
https://hdl.handle.net/21.15107/rcub_cer_6472 .

TY  - JOUR
AU  - Milošević, Dragana
AU  - Lević, Steva
AU  - Lazarević, Slavica
AU  - Veličković, Zlate
AU  - Marinković, Aleksandar D.
AU  - Petrović, Rada
AU  - Petrović, Predrag
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4811
AB  - The alkali treated subglebal tissue of the mosaic puffball (Handkea utriformis) (Sa) and Sa modified with hydroxyapatite (Sa-HAp), obtained by successive ionic layer adsorption and reaction (SILAR) method, were used for the removal of Pb2+, Cd2+ and Ni2+ from aqueous solution. The materials were characterized by FT-IR, Raman, SEM and EDS analysis and by determination of pHPZC. The adsorption performances of Sa and Sa-HAp were assessed in batch experiments at different pH, contact times, temperatures and mass of the adsorbent. Different models of adsorption isotherms were used, and the best fit was obtained with the Langmuir model. Maximum adsorption capacities of Sa towards Pb2+, Cd2+ and Ni2+ were 44.82, 15.54 and 17.21 mg g−1, while for Sa-HAp were 79.55, 52.59 and 45.01 mg g−1, respectively. Kinetic data were well fitted by a pseudo second-order model, while thermodynamic studies disclose spontaneous and endothermic adsorption process. The Sa-Hap was successfully regenerated with 1 M NaCl and after the fifth desorption cycle and 10 h achieved 82.9, 69.7 and 60.4 %, while for 0.5 M NaCl + 0.5 M NaOH and 1 h was 78.3, 64.1, 57.5 % of desorbed Pb2+, Cd2+ and Ni2+, respectively. The competitive study and results from a column system confirmed good applicability of Sa-HAp adsorbent.
PB  - Elsevier
T2  - Journal of Environmental Management
T1  - Hybrid material based on subgleba of mosaic puffball mushroom (Handkea utriformis) as an adsorbent for heavy metal removal from aqueous solutions
VL  - 297
SP  - 113358
DO  - 10.1016/j.jenvman.2021.113358
ER  - 

@article{
author = "Milošević, Dragana and Lević, Steva and Lazarević, Slavica and Veličković, Zlate and Marinković, Aleksandar D. and Petrović, Rada and Petrović, Predrag",
year = "2021",
abstract = "The alkali treated subglebal tissue of the mosaic puffball (Handkea utriformis) (Sa) and Sa modified with hydroxyapatite (Sa-HAp), obtained by successive ionic layer adsorption and reaction (SILAR) method, were used for the removal of Pb2+, Cd2+ and Ni2+ from aqueous solution. The materials were characterized by FT-IR, Raman, SEM and EDS analysis and by determination of pHPZC. The adsorption performances of Sa and Sa-HAp were assessed in batch experiments at different pH, contact times, temperatures and mass of the adsorbent. Different models of adsorption isotherms were used, and the best fit was obtained with the Langmuir model. Maximum adsorption capacities of Sa towards Pb2+, Cd2+ and Ni2+ were 44.82, 15.54 and 17.21 mg g−1, while for Sa-HAp were 79.55, 52.59 and 45.01 mg g−1, respectively. Kinetic data were well fitted by a pseudo second-order model, while thermodynamic studies disclose spontaneous and endothermic adsorption process. The Sa-Hap was successfully regenerated with 1 M NaCl and after the fifth desorption cycle and 10 h achieved 82.9, 69.7 and 60.4 %, while for 0.5 M NaCl + 0.5 M NaOH and 1 h was 78.3, 64.1, 57.5 % of desorbed Pb2+, Cd2+ and Ni2+, respectively. The competitive study and results from a column system confirmed good applicability of Sa-HAp adsorbent.",
publisher = "Elsevier",
journal = "Journal of Environmental Management",
title = "Hybrid material based on subgleba of mosaic puffball mushroom (Handkea utriformis) as an adsorbent for heavy metal removal from aqueous solutions",
volume = "297",
pages = "113358",
doi = "10.1016/j.jenvman.2021.113358"
}

Milošević, D., Lević, S., Lazarević, S., Veličković, Z., Marinković, A. D., Petrović, R.,& Petrović, P.. (2021). Hybrid material based on subgleba of mosaic puffball mushroom (Handkea utriformis) as an adsorbent for heavy metal removal from aqueous solutions. in Journal of Environmental Management
Elsevier., 297, 113358.
https://doi.org/10.1016/j.jenvman.2021.113358

Milošević D, Lević S, Lazarević S, Veličković Z, Marinković AD, Petrović R, Petrović P. Hybrid material based on subgleba of mosaic puffball mushroom (Handkea utriformis) as an adsorbent for heavy metal removal from aqueous solutions. in Journal of Environmental Management. 2021;297:113358.
doi:10.1016/j.jenvman.2021.113358 .

Milošević, Dragana, Lević, Steva, Lazarević, Slavica, Veličković, Zlate, Marinković, Aleksandar D., Petrović, Rada, Petrović, Predrag, "Hybrid material based on subgleba of mosaic puffball mushroom (Handkea utriformis) as an adsorbent for heavy metal removal from aqueous solutions" in Journal of Environmental Management, 297 (2021):113358,
https://doi.org/10.1016/j.jenvman.2021.113358 . .

TY  - JOUR
AU  - Sabovljević, Marko
AU  - Tomović, Gordana M.
AU  - Boycheva, Petya
AU  - Ivanov, Dobri
AU  - Denchev, Teodor T.
AU  - Denchev, Cvetomir M.
AU  - Stevanoski, Ivana
AU  - Marković, Aleksandra
AU  - Đurović, Sanja
AU  - Buzurović, Uroš
AU  - Yaneva, Galina
AU  - Ştefǎnuţ, Sorin
AU  - Ștefănuț, Miruna Maria
AU  - Knežević, Aleksandar
AU  - Petrović, Predrag
AU  - Assyov, Boris
AU  - Pantović, Jovana P.
AU  - Niketić, Marjan S.
AU  - Vukojičić, Snežana S.
AU  - Ion, Roxana
AU  - Tamas, Gabriela
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4626
AB  - This paper presents new records and noteworthy data on the following taxa in SE Europe and adjacent regions: parasitic fungus Antherospora hortensis, saprotrophic fungi Loweomyces fractipes and Pholiota henningsii, stonewort Chara canescens, mosses Grimmia caespiticia and Rhodobryum ontariense, fern Woodsia alpina, monocots Aegilops triuncialis, Epipactis purpurata, Galanthus elwesii and Typha shuttleworthii and dicot Umbilicus luteus.
AB  - Prikazani su novi i značajni podaci sa područja JI Evrope i susednih regiona o parazitskoj gljivi Antherospora hortensis, saprofitskim
gljivama Loweomyces fractipes i Pholiota henningsii, hari Chara canescens, mahovinama Grimmia caespiticia i Rhodobryum ontariense,
paprati Woodsia alpina, monokotilama Aegilops triuncialis, Epipactis purpurata, Galanthus elwesii i Typha shuttleworthii i
dikotili Umbilicus luteus.
PB  - Belgrade : Institute of Botany and Botanical Garden Jevremovac, Faculty of Biology
T2  - Botanica Serbica
T1  - New records and noteworthy data of plants, algae and fungi in SE Europe and adjacent regions, 3
T1  - Novi i značajni podaci o biljkama, algama i gljivama iz JI Evrope i susednih regiona, 3
VL  - 45
IS  - 1
SP  - 119
EP  - 127
DO  - 10.2298/BOTSERB2101119S
ER  - 

@article{
author = "Sabovljević, Marko and Tomović, Gordana M. and Boycheva, Petya and Ivanov, Dobri and Denchev, Teodor T. and Denchev, Cvetomir M. and Stevanoski, Ivana and Marković, Aleksandra and Đurović, Sanja and Buzurović, Uroš and Yaneva, Galina and Ştefǎnuţ, Sorin and Ștefănuț, Miruna Maria and Knežević, Aleksandar and Petrović, Predrag and Assyov, Boris and Pantović, Jovana P. and Niketić, Marjan S. and Vukojičić, Snežana S. and Ion, Roxana and Tamas, Gabriela",
year = "2021",
abstract = "This paper presents new records and noteworthy data on the following taxa in SE Europe and adjacent regions: parasitic fungus Antherospora hortensis, saprotrophic fungi Loweomyces fractipes and Pholiota henningsii, stonewort Chara canescens, mosses Grimmia caespiticia and Rhodobryum ontariense, fern Woodsia alpina, monocots Aegilops triuncialis, Epipactis purpurata, Galanthus elwesii and Typha shuttleworthii and dicot Umbilicus luteus., Prikazani su novi i značajni podaci sa područja JI Evrope i susednih regiona o parazitskoj gljivi Antherospora hortensis, saprofitskim
gljivama Loweomyces fractipes i Pholiota henningsii, hari Chara canescens, mahovinama Grimmia caespiticia i Rhodobryum ontariense,
paprati Woodsia alpina, monokotilama Aegilops triuncialis, Epipactis purpurata, Galanthus elwesii i Typha shuttleworthii i
dikotili Umbilicus luteus.",
publisher = "Belgrade : Institute of Botany and Botanical Garden Jevremovac, Faculty of Biology",
journal = "Botanica Serbica",
title = "New records and noteworthy data of plants, algae and fungi in SE Europe and adjacent regions, 3, Novi i značajni podaci o biljkama, algama i gljivama iz JI Evrope i susednih regiona, 3",
volume = "45",
number = "1",
pages = "119-127",
doi = "10.2298/BOTSERB2101119S"
}

Sabovljević, M., Tomović, G. M., Boycheva, P., Ivanov, D., Denchev, T. T., Denchev, C. M., Stevanoski, I., Marković, A., Đurović, S., Buzurović, U., Yaneva, G., Ştefǎnuţ, S., Ștefănuț, M. M., Knežević, A., Petrović, P., Assyov, B., Pantović, J. P., Niketić, M. S., Vukojičić, S. S., Ion, R.,& Tamas, G.. (2021). New records and noteworthy data of plants, algae and fungi in SE Europe and adjacent regions, 3. in Botanica Serbica
Belgrade : Institute of Botany and Botanical Garden Jevremovac, Faculty of Biology., 45(1), 119-127.
https://doi.org/10.2298/BOTSERB2101119S

Sabovljević M, Tomović GM, Boycheva P, Ivanov D, Denchev TT, Denchev CM, Stevanoski I, Marković A, Đurović S, Buzurović U, Yaneva G, Ştefǎnuţ S, Ștefănuț MM, Knežević A, Petrović P, Assyov B, Pantović JP, Niketić MS, Vukojičić SS, Ion R, Tamas G. New records and noteworthy data of plants, algae and fungi in SE Europe and adjacent regions, 3. in Botanica Serbica. 2021;45(1):119-127.
doi:10.2298/BOTSERB2101119S .

Sabovljević, Marko, Tomović, Gordana M., Boycheva, Petya, Ivanov, Dobri, Denchev, Teodor T., Denchev, Cvetomir M., Stevanoski, Ivana, Marković, Aleksandra, Đurović, Sanja, Buzurović, Uroš, Yaneva, Galina, Ştefǎnuţ, Sorin, Ștefănuț, Miruna Maria, Knežević, Aleksandar, Petrović, Predrag, Assyov, Boris, Pantović, Jovana P., Niketić, Marjan S., Vukojičić, Snežana S., Ion, Roxana, Tamas, Gabriela, "New records and noteworthy data of plants, algae and fungi in SE Europe and adjacent regions, 3" in Botanica Serbica, 45, no. 1 (2021):119-127,
https://doi.org/10.2298/BOTSERB2101119S . .

TY  - CONF
AU  - Milošević, Dragana
AU  - Kovačina, Jovanka
AU  - Bugarčić, Mladen
AU  - Simović, Anđela
AU  - Petrović, Predrag
AU  - Marinković, Aleksandar
AU  - Petrović, Rada
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6470
AB  - Mushrooms are widely investigated and recognized as perspective materials for the removal
of various pollutants from wastewater effluents. In this study, mosaic puffball Handkea
utriformis was tested as a new biodegradable, but relatively stable material for Cd2+
adsorption from aqueous mediums. The fruiting body of Handkea utriformis goes through the
process of autodigestion, during which their inside – gleba is turned into a powdery, sporebearing mass, and the lower portion of the fruiting body – subgleba, into a dark, dry, spongy
mass. The impurities and spores present on the surface of the subgleba can be removed in a
simple process of purification with acidic and alkaline solutions. The purification decreases
the hydrophobicity of the surface of this material, and therefore increases the number of
surface functionalities, knowing that subgleba consists of sugars, proteins and polymeric
pigments (melanins). The subgleba purified with 0.1M HCl and 0.1M NaOH (Sp) was used
for the removal of Cd2+ from the aqueous solution. The efficiency of the material for
adsorption of Cd2+ was investigated in a batch system under the constant initial concentration
of adsorbate and pH and different concentrations of the adsorbent. Composition and surface
morphology were characterized by using FT-IR spectroscopy, SEM and EDX analysis.
Concentrations of Cd2+, before and after adsorption, were determined by using Atomic
Absorption Spectroscopy (AAS). The Langmuir and Freundlich isotherm models, as well as
kinetic models, were evaluated to correlate experimental data. The results showed that kinetic
data were well fitted by a pseudo-second-order model. Isotherms studies revealed that the
best fit was achieved with the Langmuir isotherm model with a maximum adsorption capacity
of 15.2, 19.1 and 25.0 mg g-1 at 25, 35 and 45ºC, respectively.
PB  - Zvornik : Faculty of Technology
C3  - Book of Abstracts - VII International Congress Engineering, Environment and Materials in Process Industry, 17th-19th March 2021, Jahorina - Republic of Srpska, Bosnia and Herzegovina
T1  - Efficient removal of Cd2+ from aqueous solution using subgleba of mushroom Handkea utriformis
SP  - 73
EP  - 73
UR  - https://hdl.handle.net/21.15107/rcub_cer_6470
ER  - 

@conference{
author = "Milošević, Dragana and Kovačina, Jovanka and Bugarčić, Mladen and Simović, Anđela and Petrović, Predrag and Marinković, Aleksandar and Petrović, Rada",
year = "2021",
abstract = "Mushrooms are widely investigated and recognized as perspective materials for the removal
of various pollutants from wastewater effluents. In this study, mosaic puffball Handkea
utriformis was tested as a new biodegradable, but relatively stable material for Cd2+
adsorption from aqueous mediums. The fruiting body of Handkea utriformis goes through the
process of autodigestion, during which their inside – gleba is turned into a powdery, sporebearing mass, and the lower portion of the fruiting body – subgleba, into a dark, dry, spongy
mass. The impurities and spores present on the surface of the subgleba can be removed in a
simple process of purification with acidic and alkaline solutions. The purification decreases
the hydrophobicity of the surface of this material, and therefore increases the number of
surface functionalities, knowing that subgleba consists of sugars, proteins and polymeric
pigments (melanins). The subgleba purified with 0.1M HCl and 0.1M NaOH (Sp) was used
for the removal of Cd2+ from the aqueous solution. The efficiency of the material for
adsorption of Cd2+ was investigated in a batch system under the constant initial concentration
of adsorbate and pH and different concentrations of the adsorbent. Composition and surface
morphology were characterized by using FT-IR spectroscopy, SEM and EDX analysis.
Concentrations of Cd2+, before and after adsorption, were determined by using Atomic
Absorption Spectroscopy (AAS). The Langmuir and Freundlich isotherm models, as well as
kinetic models, were evaluated to correlate experimental data. The results showed that kinetic
data were well fitted by a pseudo-second-order model. Isotherms studies revealed that the
best fit was achieved with the Langmuir isotherm model with a maximum adsorption capacity
of 15.2, 19.1 and 25.0 mg g-1 at 25, 35 and 45ºC, respectively.",
publisher = "Zvornik : Faculty of Technology",
journal = "Book of Abstracts - VII International Congress Engineering, Environment and Materials in Process Industry, 17th-19th March 2021, Jahorina - Republic of Srpska, Bosnia and Herzegovina",
title = "Efficient removal of Cd2+ from aqueous solution using subgleba of mushroom Handkea utriformis",
pages = "73-73",
url = "https://hdl.handle.net/21.15107/rcub_cer_6470"
}

Milošević, D., Kovačina, J., Bugarčić, M., Simović, A., Petrović, P., Marinković, A.,& Petrović, R.. (2021). Efficient removal of Cd2+ from aqueous solution using subgleba of mushroom Handkea utriformis. in Book of Abstracts - VII International Congress Engineering, Environment and Materials in Process Industry, 17th-19th March 2021, Jahorina - Republic of Srpska, Bosnia and Herzegovina
Zvornik : Faculty of Technology., 73-73.
https://hdl.handle.net/21.15107/rcub_cer_6470

Milošević D, Kovačina J, Bugarčić M, Simović A, Petrović P, Marinković A, Petrović R. Efficient removal of Cd2+ from aqueous solution using subgleba of mushroom Handkea utriformis. in Book of Abstracts - VII International Congress Engineering, Environment and Materials in Process Industry, 17th-19th March 2021, Jahorina - Republic of Srpska, Bosnia and Herzegovina. 2021;:73-73.
https://hdl.handle.net/21.15107/rcub_cer_6470 .

Milošević, Dragana, Kovačina, Jovanka, Bugarčić, Mladen, Simović, Anđela, Petrović, Predrag, Marinković, Aleksandar, Petrović, Rada, "Efficient removal of Cd2+ from aqueous solution using subgleba of mushroom Handkea utriformis" in Book of Abstracts - VII International Congress Engineering, Environment and Materials in Process Industry, 17th-19th March 2021, Jahorina - Republic of Srpska, Bosnia and Herzegovina (2021):73-73,
https://hdl.handle.net/21.15107/rcub_cer_6470 .

TY  - JOUR
AU  - Milošević, Milena D.
AU  - Marinković, Aleksandar D.
AU  - Petrović, Predrag
AU  - Klaus, Anita
AU  - Nikolić, Milica G.
AU  - Prlainović, Nevena Ž.
AU  - Cvijetić, Ilija
PY  - 2020
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3627
AB  - In this study we synthesized a series of sixteen bis(imino)pyridines (BIPs) starting from 2,6-diaminopyridine and various aromatic aldehydes, and evaluated their antioxidant, antibacterial, antifungal and acetylcholinesterase (AChE) inhibitory activity. The chemical structures were elucidated by FTIR, elemental analysis, ESR and HRMS. 1H and 13C NMR spectra couldn’t be acquired due to the formation of stable, carbon-centered radical cations in a solution, as confirmed by ESR spectroscopy and DFT calculations. The in vitro antioxidant potency was evaluated using four assays: free radical scavenging activity (DPPH and ABTS), reducing power and total antioxidant capacity assay. BIPs demonstrated excellent antioxidant properties, and two derivatives proved to be more potent than reference antioxidants (ascorbic acid and Trolox) in all assays. DFT calculations on ωB97XD/6-311++g(d,p) level of theory provided valuable insights into the radical scavenging mechanism of BIPs. For hydroxyl-substituted BIPs, hydrogen atom transfer (HAT) is a predominant mechanism, while the single electron transfer coupled with proton transfer (SET-PT) governs the antioxidant activity of other derivatives. Intramolecular hydrogen bonding (IHB) plays an important role in the mechanism of antioxidant activity as revealed by noncovalent interaction analysis and rotational barrier calculations. The spin density of radical cations is localized on carbon atoms of a pyridine ring, which corroborates with g-factors and multiplicity obtained from ESR analysis. The most potent BIP exhibited moderate inhibitory activity toward AChE (IC50=20 ± 4 μM), while molecular docking suggested binding at the peripheral anionic site of AChE with the MMFF94 binding enthalpy of −43.4 kcal/mol. Moderate in vitro antimicrobial activity of BIPs have been determined against several pathogenic bacterial strains: Pseudomonas aeruginosa, Escherichia coli, Enterococcus faecalis, Staphylococcus aureus and clinical isolate of methicillin resistant S. aureus (MRSA). The antifungal activity of BIPs toward Candida albicans was also confirmed. The similarity ensemble approach combined with molecular docking suggested leucyl aminopeptidase as the probable antimicrobial target for the three most potent BIP derivatives.
PB  - Elsevier
T2  - Bioorganic Chemistry
T1  - Synthesis, characterization and SAR studies of bis(imino)pyridines as antioxidants, acetylcholinesterase inhibitors and antimicrobial agents
VL  - 102
SP  - 104073
DO  - 10.1016/j.bioorg.2020.104073
ER  - 

@article{
author = "Milošević, Milena D. and Marinković, Aleksandar D. and Petrović, Predrag and Klaus, Anita and Nikolić, Milica G. and Prlainović, Nevena Ž. and Cvijetić, Ilija",
year = "2020",
abstract = "In this study we synthesized a series of sixteen bis(imino)pyridines (BIPs) starting from 2,6-diaminopyridine and various aromatic aldehydes, and evaluated their antioxidant, antibacterial, antifungal and acetylcholinesterase (AChE) inhibitory activity. The chemical structures were elucidated by FTIR, elemental analysis, ESR and HRMS. 1H and 13C NMR spectra couldn’t be acquired due to the formation of stable, carbon-centered radical cations in a solution, as confirmed by ESR spectroscopy and DFT calculations. The in vitro antioxidant potency was evaluated using four assays: free radical scavenging activity (DPPH and ABTS), reducing power and total antioxidant capacity assay. BIPs demonstrated excellent antioxidant properties, and two derivatives proved to be more potent than reference antioxidants (ascorbic acid and Trolox) in all assays. DFT calculations on ωB97XD/6-311++g(d,p) level of theory provided valuable insights into the radical scavenging mechanism of BIPs. For hydroxyl-substituted BIPs, hydrogen atom transfer (HAT) is a predominant mechanism, while the single electron transfer coupled with proton transfer (SET-PT) governs the antioxidant activity of other derivatives. Intramolecular hydrogen bonding (IHB) plays an important role in the mechanism of antioxidant activity as revealed by noncovalent interaction analysis and rotational barrier calculations. The spin density of radical cations is localized on carbon atoms of a pyridine ring, which corroborates with g-factors and multiplicity obtained from ESR analysis. The most potent BIP exhibited moderate inhibitory activity toward AChE (IC50=20 ± 4 μM), while molecular docking suggested binding at the peripheral anionic site of AChE with the MMFF94 binding enthalpy of −43.4 kcal/mol. Moderate in vitro antimicrobial activity of BIPs have been determined against several pathogenic bacterial strains: Pseudomonas aeruginosa, Escherichia coli, Enterococcus faecalis, Staphylococcus aureus and clinical isolate of methicillin resistant S. aureus (MRSA). The antifungal activity of BIPs toward Candida albicans was also confirmed. The similarity ensemble approach combined with molecular docking suggested leucyl aminopeptidase as the probable antimicrobial target for the three most potent BIP derivatives.",
publisher = "Elsevier",
journal = "Bioorganic Chemistry",
title = "Synthesis, characterization and SAR studies of bis(imino)pyridines as antioxidants, acetylcholinesterase inhibitors and antimicrobial agents",
volume = "102",
pages = "104073",
doi = "10.1016/j.bioorg.2020.104073"
}

Milošević, M. D., Marinković, A. D., Petrović, P., Klaus, A., Nikolić, M. G., Prlainović, N. Ž.,& Cvijetić, I.. (2020). Synthesis, characterization and SAR studies of bis(imino)pyridines as antioxidants, acetylcholinesterase inhibitors and antimicrobial agents. in Bioorganic Chemistry
Elsevier., 102, 104073.
https://doi.org/10.1016/j.bioorg.2020.104073

Milošević MD, Marinković AD, Petrović P, Klaus A, Nikolić MG, Prlainović NŽ, Cvijetić I. Synthesis, characterization and SAR studies of bis(imino)pyridines as antioxidants, acetylcholinesterase inhibitors and antimicrobial agents. in Bioorganic Chemistry. 2020;102:104073.
doi:10.1016/j.bioorg.2020.104073 .

Milošević, Milena D., Marinković, Aleksandar D., Petrović, Predrag, Klaus, Anita, Nikolić, Milica G., Prlainović, Nevena Ž., Cvijetić, Ilija, "Synthesis, characterization and SAR studies of bis(imino)pyridines as antioxidants, acetylcholinesterase inhibitors and antimicrobial agents" in Bioorganic Chemistry, 102 (2020):104073,
https://doi.org/10.1016/j.bioorg.2020.104073 . .

TY  - JOUR
AU  - Milošević, Milena D.
AU  - Marinković, Aleksandar D.
AU  - Petrović, Predrag
AU  - Klaus, Anita
AU  - Nikolić, Milica G.
AU  - Prlainović, Nevena Ž.
AU  - Cvijetić, Ilija
PY  - 2020
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3630
AB  - In this study we synthesized a series of sixteen bis(imino)pyridines (BIPs) starting from 2,6-diaminopyridine and various aromatic aldehydes, and evaluated their antioxidant, antibacterial, antifungal and acetylcholinesterase (AChE) inhibitory activity. The chemical structures were elucidated by FTIR, elemental analysis, ESR and HRMS. 1H and 13C NMR spectra couldn’t be acquired due to the formation of stable, carbon-centered radical cations in a solution, as confirmed by ESR spectroscopy and DFT calculations. The in vitro antioxidant potency was evaluated using four assays: free radical scavenging activity (DPPH and ABTS), reducing power and total antioxidant capacity assay. BIPs demonstrated excellent antioxidant properties, and two derivatives proved to be more potent than reference antioxidants (ascorbic acid and Trolox) in all assays. DFT calculations on ωB97XD/6-311++g(d,p) level of theory provided valuable insights into the radical scavenging mechanism of BIPs. For hydroxyl-substituted BIPs, hydrogen atom transfer (HAT) is a predominant mechanism, while the single electron transfer coupled with proton transfer (SET-PT) governs the antioxidant activity of other derivatives. Intramolecular hydrogen bonding (IHB) plays an important role in the mechanism of antioxidant activity as revealed by noncovalent interaction analysis and rotational barrier calculations. The spin density of radical cations is localized on carbon atoms of a pyridine ring, which corroborates with g-factors and multiplicity obtained from ESR analysis. The most potent BIP exhibited moderate inhibitory activity toward AChE (IC50=20 ± 4 μM), while molecular docking suggested binding at the peripheral anionic site of AChE with theMMFF94 binding enthalpy of −43.4 kcal/mol. Moderate in vitro antimicrobial activity of BIPs have been determined against several pathogenic bacterial strains: Pseudomonas aeruginosa, Escherichia coli, Enterococcus faecalis, Staphylococcus aureus and clinical isolate of methicillin resistant S. aureus (MRSA). The antifungal activity of BIPs toward Candida albicans was also confirmed. The similarity ensemble approach combined with molecular docking suggested leucyl aminopeptidase as the probable antimicrobial target for the three most potent BIP derivatives.
PB  - Elsevier
T2  - Bioorganic Chemistry
T1  - Synthesis, characterization and SAR studies of bis(imino)pyridines as antioxidants, acetylcholinesterase inhibitors and antimicrobial agents
VL  - 102
SP  - 104073
DO  - 10.1016/j.bioorg.2020.104073
ER  - 

@article{
author = "Milošević, Milena D. and Marinković, Aleksandar D. and Petrović, Predrag and Klaus, Anita and Nikolić, Milica G. and Prlainović, Nevena Ž. and Cvijetić, Ilija",
year = "2020",
abstract = "In this study we synthesized a series of sixteen bis(imino)pyridines (BIPs) starting from 2,6-diaminopyridine and various aromatic aldehydes, and evaluated their antioxidant, antibacterial, antifungal and acetylcholinesterase (AChE) inhibitory activity. The chemical structures were elucidated by FTIR, elemental analysis, ESR and HRMS. 1H and 13C NMR spectra couldn’t be acquired due to the formation of stable, carbon-centered radical cations in a solution, as confirmed by ESR spectroscopy and DFT calculations. The in vitro antioxidant potency was evaluated using four assays: free radical scavenging activity (DPPH and ABTS), reducing power and total antioxidant capacity assay. BIPs demonstrated excellent antioxidant properties, and two derivatives proved to be more potent than reference antioxidants (ascorbic acid and Trolox) in all assays. DFT calculations on ωB97XD/6-311++g(d,p) level of theory provided valuable insights into the radical scavenging mechanism of BIPs. For hydroxyl-substituted BIPs, hydrogen atom transfer (HAT) is a predominant mechanism, while the single electron transfer coupled with proton transfer (SET-PT) governs the antioxidant activity of other derivatives. Intramolecular hydrogen bonding (IHB) plays an important role in the mechanism of antioxidant activity as revealed by noncovalent interaction analysis and rotational barrier calculations. The spin density of radical cations is localized on carbon atoms of a pyridine ring, which corroborates with g-factors and multiplicity obtained from ESR analysis. The most potent BIP exhibited moderate inhibitory activity toward AChE (IC50=20 ± 4 μM), while molecular docking suggested binding at the peripheral anionic site of AChE with theMMFF94 binding enthalpy of −43.4 kcal/mol. Moderate in vitro antimicrobial activity of BIPs have been determined against several pathogenic bacterial strains: Pseudomonas aeruginosa, Escherichia coli, Enterococcus faecalis, Staphylococcus aureus and clinical isolate of methicillin resistant S. aureus (MRSA). The antifungal activity of BIPs toward Candida albicans was also confirmed. The similarity ensemble approach combined with molecular docking suggested leucyl aminopeptidase as the probable antimicrobial target for the three most potent BIP derivatives.",
publisher = "Elsevier",
journal = "Bioorganic Chemistry",
title = "Synthesis, characterization and SAR studies of bis(imino)pyridines as antioxidants, acetylcholinesterase inhibitors and antimicrobial agents",
volume = "102",
pages = "104073",
doi = "10.1016/j.bioorg.2020.104073"
}

Milošević, M. D., Marinković, A. D., Petrović, P., Klaus, A., Nikolić, M. G., Prlainović, N. Ž.,& Cvijetić, I.. (2020). Synthesis, characterization and SAR studies of bis(imino)pyridines as antioxidants, acetylcholinesterase inhibitors and antimicrobial agents. in Bioorganic Chemistry
Elsevier., 102, 104073.
https://doi.org/10.1016/j.bioorg.2020.104073

Milošević MD, Marinković AD, Petrović P, Klaus A, Nikolić MG, Prlainović NŽ, Cvijetić I. Synthesis, characterization and SAR studies of bis(imino)pyridines as antioxidants, acetylcholinesterase inhibitors and antimicrobial agents. in Bioorganic Chemistry. 2020;102:104073.
doi:10.1016/j.bioorg.2020.104073 .

Milošević, Milena D., Marinković, Aleksandar D., Petrović, Predrag, Klaus, Anita, Nikolić, Milica G., Prlainović, Nevena Ž., Cvijetić, Ilija, "Synthesis, characterization and SAR studies of bis(imino)pyridines as antioxidants, acetylcholinesterase inhibitors and antimicrobial agents" in Bioorganic Chemistry, 102 (2020):104073,
https://doi.org/10.1016/j.bioorg.2020.104073 . .

TY  - DATA
AU  - Božić, Aleksandra R.
AU  - Bjelogrlić, Snežana K.
AU  - Novaković, Irena
AU  - Filipovic, Nenad R.
AU  - Petrović, Predrag M.
AU  - Marinković, Aleksandar D.
AU  - Todorović, Tamara
AU  - Cvijetić, Ilija
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4480
AB  - Table S1. Statistics of the PCA model generated after 2 FFD cycle. Table S2. Statistics of 3LV PLS model applying different CV procedures. Table S3. Statistics of the PCA model generated after 2 FFD cycle. Table S4. Statistics of 2LV PLS model applying different CV procedures. Experimental Section: materials, methods, general procedure for synthesis of m-TCHs 1-11, general procedure for synthesis of b-TCHs 12-22,. Antimicrobial activity: agar diffusion method, Broth microdilution antimicrobial assay. Alignment-independent 3D QSAR models. Figure S1. 1H NMR spectrum of 3 in DMSO-d6. Figure S2.13C NMR spectrum of 3 in DMSO-d6. Figure S3. 1H NMR spectrum of 4 in DMSO-d6. Figure S4.13C NMR spectrum of 4 in DMSO-d6. Figure S5. 1H NMR spectrum of 6 in DMSO-d6. Figure S6.13C NMR spectrum of 6 in DMSO-d6. Additional references.
PB  - Wiley
T2  - Chemistryselect
T1  - Supporting information for: "Antimicrobial Activity of Thiocarbohydrazones: Experimental Studies and Alignment-Independent 3D QSAR Models"
UR  - https://hdl.handle.net/21.15107/rcub_cer_4480
ER  - 

@misc{
author = "Božić, Aleksandra R. and Bjelogrlić, Snežana K. and Novaković, Irena and Filipovic, Nenad R. and Petrović, Predrag M. and Marinković, Aleksandar D. and Todorović, Tamara and Cvijetić, Ilija",
year = "2018",
abstract = "Table S1. Statistics of the PCA model generated after 2 FFD cycle. Table S2. Statistics of 3LV PLS model applying different CV procedures. Table S3. Statistics of the PCA model generated after 2 FFD cycle. Table S4. Statistics of 2LV PLS model applying different CV procedures. Experimental Section: materials, methods, general procedure for synthesis of m-TCHs 1-11, general procedure for synthesis of b-TCHs 12-22,. Antimicrobial activity: agar diffusion method, Broth microdilution antimicrobial assay. Alignment-independent 3D QSAR models. Figure S1. 1H NMR spectrum of 3 in DMSO-d6. Figure S2.13C NMR spectrum of 3 in DMSO-d6. Figure S3. 1H NMR spectrum of 4 in DMSO-d6. Figure S4.13C NMR spectrum of 4 in DMSO-d6. Figure S5. 1H NMR spectrum of 6 in DMSO-d6. Figure S6.13C NMR spectrum of 6 in DMSO-d6. Additional references.",
publisher = "Wiley",
journal = "Chemistryselect",
title = "Supporting information for: "Antimicrobial Activity of Thiocarbohydrazones: Experimental Studies and Alignment-Independent 3D QSAR Models"",
url = "https://hdl.handle.net/21.15107/rcub_cer_4480"
}

Božić, A. R., Bjelogrlić, S. K., Novaković, I., Filipovic, N. R., Petrović, P. M., Marinković, A. D., Todorović, T.,& Cvijetić, I.. (2018). Supporting information for: "Antimicrobial Activity of Thiocarbohydrazones: Experimental Studies and Alignment-Independent 3D QSAR Models". in Chemistryselect
Wiley..
https://hdl.handle.net/21.15107/rcub_cer_4480

Božić AR, Bjelogrlić SK, Novaković I, Filipovic NR, Petrović PM, Marinković AD, Todorović T, Cvijetić I. Supporting information for: "Antimicrobial Activity of Thiocarbohydrazones: Experimental Studies and Alignment-Independent 3D QSAR Models". in Chemistryselect. 2018;.
https://hdl.handle.net/21.15107/rcub_cer_4480 .

Božić, Aleksandra R., Bjelogrlić, Snežana K., Novaković, Irena, Filipovic, Nenad R., Petrović, Predrag M., Marinković, Aleksandar D., Todorović, Tamara, Cvijetić, Ilija, "Supporting information for: "Antimicrobial Activity of Thiocarbohydrazones: Experimental Studies and Alignment-Independent 3D QSAR Models"" in Chemistryselect (2018),
https://hdl.handle.net/21.15107/rcub_cer_4480 .

TY  - JOUR
AU  - Božić, Aleksandra R.
AU  - Bjelogrlić, Snežana K.
AU  - Novaković, Irena
AU  - Filipovic, Nenad R.
AU  - Petrović, Predrag M.
AU  - Marinković, Aleksandar D.
AU  - Todorović, Tamara
AU  - Cvijetić, Ilija
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2329
AB  - Due to the rise of microbial strains resistant to conventional therapies, there is an urgent need for finding the new antimicrobial chemotypes. Heterocyclic compounds such as thiocarbohydrazones (TCHs) are able to interact with many metalloenzymes essential for microbes, while sulfur atom increases lipophilicity which is generally positively correlated with potency. In this paper, we report antibacterial and antifungal activity of twenty-two TCHs toward eight bacterial and three fungal strains. Furthermore, three alignment independent 3D QSAR models based on descriptors derived from molecular interaction fields (MIFs) are developed in order to rationalize structure-activity relationships for activities of TCHs toward S. aureus, P. aeruginosa and C. albicans. Several structural fragments important for biological activity are recognized in each model, and structural modifications which could lead to increased potency are suggested. Designed structures will be synthesized accordingly and tested toward the same microbial strains in order to obtain more potent derivatives.
PB  - Wiley-V C H Verlag Gmbh, Weinheim
T2  - Chemistryselect
T1  - Antimicrobial Activity of Thiocarbohydrazones: Experimental Studies and Alignment-Independent 3D QSAR Models
VL  - 3
IS  - 7
SP  - 2215
EP  - 2221
DO  - 10.1002/slct.201702691
ER  - 

@article{
author = "Božić, Aleksandra R. and Bjelogrlić, Snežana K. and Novaković, Irena and Filipovic, Nenad R. and Petrović, Predrag M. and Marinković, Aleksandar D. and Todorović, Tamara and Cvijetić, Ilija",
year = "2018",
abstract = "Due to the rise of microbial strains resistant to conventional therapies, there is an urgent need for finding the new antimicrobial chemotypes. Heterocyclic compounds such as thiocarbohydrazones (TCHs) are able to interact with many metalloenzymes essential for microbes, while sulfur atom increases lipophilicity which is generally positively correlated with potency. In this paper, we report antibacterial and antifungal activity of twenty-two TCHs toward eight bacterial and three fungal strains. Furthermore, three alignment independent 3D QSAR models based on descriptors derived from molecular interaction fields (MIFs) are developed in order to rationalize structure-activity relationships for activities of TCHs toward S. aureus, P. aeruginosa and C. albicans. Several structural fragments important for biological activity are recognized in each model, and structural modifications which could lead to increased potency are suggested. Designed structures will be synthesized accordingly and tested toward the same microbial strains in order to obtain more potent derivatives.",
publisher = "Wiley-V C H Verlag Gmbh, Weinheim",
journal = "Chemistryselect",
title = "Antimicrobial Activity of Thiocarbohydrazones: Experimental Studies and Alignment-Independent 3D QSAR Models",
volume = "3",
number = "7",
pages = "2215-2221",
doi = "10.1002/slct.201702691"
}

Božić, A. R., Bjelogrlić, S. K., Novaković, I., Filipovic, N. R., Petrović, P. M., Marinković, A. D., Todorović, T.,& Cvijetić, I.. (2018). Antimicrobial Activity of Thiocarbohydrazones: Experimental Studies and Alignment-Independent 3D QSAR Models. in Chemistryselect
Wiley-V C H Verlag Gmbh, Weinheim., 3(7), 2215-2221.
https://doi.org/10.1002/slct.201702691

Božić AR, Bjelogrlić SK, Novaković I, Filipovic NR, Petrović PM, Marinković AD, Todorović T, Cvijetić I. Antimicrobial Activity of Thiocarbohydrazones: Experimental Studies and Alignment-Independent 3D QSAR Models. in Chemistryselect. 2018;3(7):2215-2221.
doi:10.1002/slct.201702691 .

Božić, Aleksandra R., Bjelogrlić, Snežana K., Novaković, Irena, Filipovic, Nenad R., Petrović, Predrag M., Marinković, Aleksandar D., Todorović, Tamara, Cvijetić, Ilija, "Antimicrobial Activity of Thiocarbohydrazones: Experimental Studies and Alignment-Independent 3D QSAR Models" in Chemistryselect, 3, no. 7 (2018):2215-2221,
https://doi.org/10.1002/slct.201702691 . .

TY  - JOUR
AU  - Petrović, Predrag
AU  - Vunduk, Jovana
AU  - Klaus, Anita
AU  - Kozarski, Maja S.
AU  - Nikšić, Miomir
AU  - Žižak, Željko
AU  - Vukovic, Nebojsa
AU  - Šekularac, Gavrilo
AU  - Drmanić, Saša Ž.
AU  - Bugarski, Branko
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1966
AB  - Handkea utriformis (HU), H. excipuliformis (HE) and Vascellum pratense (VP) mature fruiting bodies methanol extracts were tested for biological activities and active compounds. The extracts showed prominent radical scavenging, reducing, antioxidative and chelating abilities. Scavenging ability was correlated with phenolic (ABTS assay) or phenolic and/or sugar/beta-glucan content (DPPH). Antioxidative and Fe3+-reducing ability of VP extract was the highest, and was best correlated with flavonoid content. The same extract exhibited the best angiotensin-converting enzyme inhibitory activity. HU and HE showed selectivity toward tumor cell lines in cytotoxicity analysis. The extracts exhibited various antimicrobial activity, the best being against Listeria monocytogenes (HE, MIC-0.625 mg/mL); fatty acid content was particularly high in HU (37.25 mg/g), with linoleic acid making up more than 57% in all samples. Phenolics were present in considerable amount, as well as beta-glucans (HU, 16.67%). Although these mushrooms are inedible after autolysis process, they were still a good source of biologically active products.
PB  - Elsevier
T2  - Journal of Functional Foods
T1  - Biological potential of puffballs: A comparative analysis
VL  - 21
SP  - 36
EP  - 49
DO  - 10.1016/j.jff.2015.11.039
ER  - 

@article{
author = "Petrović, Predrag and Vunduk, Jovana and Klaus, Anita and Kozarski, Maja S. and Nikšić, Miomir and Žižak, Željko and Vukovic, Nebojsa and Šekularac, Gavrilo and Drmanić, Saša Ž. and Bugarski, Branko",
year = "2016",
abstract = "Handkea utriformis (HU), H. excipuliformis (HE) and Vascellum pratense (VP) mature fruiting bodies methanol extracts were tested for biological activities and active compounds. The extracts showed prominent radical scavenging, reducing, antioxidative and chelating abilities. Scavenging ability was correlated with phenolic (ABTS assay) or phenolic and/or sugar/beta-glucan content (DPPH). Antioxidative and Fe3+-reducing ability of VP extract was the highest, and was best correlated with flavonoid content. The same extract exhibited the best angiotensin-converting enzyme inhibitory activity. HU and HE showed selectivity toward tumor cell lines in cytotoxicity analysis. The extracts exhibited various antimicrobial activity, the best being against Listeria monocytogenes (HE, MIC-0.625 mg/mL); fatty acid content was particularly high in HU (37.25 mg/g), with linoleic acid making up more than 57% in all samples. Phenolics were present in considerable amount, as well as beta-glucans (HU, 16.67%). Although these mushrooms are inedible after autolysis process, they were still a good source of biologically active products.",
publisher = "Elsevier",
journal = "Journal of Functional Foods",
title = "Biological potential of puffballs: A comparative analysis",
volume = "21",
pages = "36-49",
doi = "10.1016/j.jff.2015.11.039"
}

Petrović, P., Vunduk, J., Klaus, A., Kozarski, M. S., Nikšić, M., Žižak, Ž., Vukovic, N., Šekularac, G., Drmanić, S. Ž.,& Bugarski, B.. (2016). Biological potential of puffballs: A comparative analysis. in Journal of Functional Foods
Elsevier., 21, 36-49.
https://doi.org/10.1016/j.jff.2015.11.039

Petrović P, Vunduk J, Klaus A, Kozarski MS, Nikšić M, Žižak Ž, Vukovic N, Šekularac G, Drmanić SŽ, Bugarski B. Biological potential of puffballs: A comparative analysis. in Journal of Functional Foods. 2016;21:36-49.
doi:10.1016/j.jff.2015.11.039 .

Petrović, Predrag, Vunduk, Jovana, Klaus, Anita, Kozarski, Maja S., Nikšić, Miomir, Žižak, Željko, Vukovic, Nebojsa, Šekularac, Gavrilo, Drmanić, Saša Ž., Bugarski, Branko, "Biological potential of puffballs: A comparative analysis" in Journal of Functional Foods, 21 (2016):36-49,
https://doi.org/10.1016/j.jff.2015.11.039 . .

TY  - JOUR
AU  - Kozarski, Maja S.
AU  - Klaus, Anita
AU  - Jakovljević, Dragica
AU  - Todorović, Nina
AU  - Vunduk, Jovana
AU  - Petrović, Predrag
AU  - Nikšić, Miomir
AU  - Vrvić, Miroslav
AU  - Van Griensven, Leo J. L. D.
PY  - 2015
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1826
AB  - Oxidative stress caused by an imbalanced metabolism and an excess of reactive oxygen species (ROS) lead to a range of health disorders in humans. Our endogenous antioxidant defense mechanisms and our dietary intake of antioxidants potentially regulate our oxidative homeostasis. Numerous synthetic antioxidants can effectively improve defense mechanisms, but because of their adverse toxic effects under certain conditions, preference is given to natural compounds. Consequently, the requirements for natural, alternative sources of antioxidant foods identified in edible mushrooms, as well as the mechanistic action involved in their antioxidant properties, have increased rapidly. Chemical composition and antioxidant potential of mushrooms have been intensively studied. Edible mushrooms might be used directly in enhancement of antioxidant defenses through dietary supplementation to reduce the level of oxidative stress. Wild or cultivated, they have been related to significant antioxidant properties due to their bioactive compounds, such as polyphenols, polysaccharides, vitamins, carotenoids and minerals. Antioxidant and health benefits, observed in edible mushrooms, seem an additional reason for their traditional use as a popular delicacy food. This review discusses the consumption of edible mushrooms as a powerful instrument in maintaining health, longevity and life quality.
PB  - MDPI
T2  - Molecules
T1  - Antioxidants of Edible Mushrooms
VL  - 20
IS  - 10
SP  - 19489
EP  - 19525
DO  - 10.3390/molecules201019489
ER  - 

@article{
author = "Kozarski, Maja S. and Klaus, Anita and Jakovljević, Dragica and Todorović, Nina and Vunduk, Jovana and Petrović, Predrag and Nikšić, Miomir and Vrvić, Miroslav and Van Griensven, Leo J. L. D.",
year = "2015",
abstract = "Oxidative stress caused by an imbalanced metabolism and an excess of reactive oxygen species (ROS) lead to a range of health disorders in humans. Our endogenous antioxidant defense mechanisms and our dietary intake of antioxidants potentially regulate our oxidative homeostasis. Numerous synthetic antioxidants can effectively improve defense mechanisms, but because of their adverse toxic effects under certain conditions, preference is given to natural compounds. Consequently, the requirements for natural, alternative sources of antioxidant foods identified in edible mushrooms, as well as the mechanistic action involved in their antioxidant properties, have increased rapidly. Chemical composition and antioxidant potential of mushrooms have been intensively studied. Edible mushrooms might be used directly in enhancement of antioxidant defenses through dietary supplementation to reduce the level of oxidative stress. Wild or cultivated, they have been related to significant antioxidant properties due to their bioactive compounds, such as polyphenols, polysaccharides, vitamins, carotenoids and minerals. Antioxidant and health benefits, observed in edible mushrooms, seem an additional reason for their traditional use as a popular delicacy food. This review discusses the consumption of edible mushrooms as a powerful instrument in maintaining health, longevity and life quality.",
publisher = "MDPI",
journal = "Molecules",
title = "Antioxidants of Edible Mushrooms",
volume = "20",
number = "10",
pages = "19489-19525",
doi = "10.3390/molecules201019489"
}

Kozarski, M. S., Klaus, A., Jakovljević, D., Todorović, N., Vunduk, J., Petrović, P., Nikšić, M., Vrvić, M.,& Van Griensven, L. J. L. D.. (2015). Antioxidants of Edible Mushrooms. in Molecules
MDPI., 20(10), 19489-19525.
https://doi.org/10.3390/molecules201019489

Kozarski MS, Klaus A, Jakovljević D, Todorović N, Vunduk J, Petrović P, Nikšić M, Vrvić M, Van Griensven LJLD. Antioxidants of Edible Mushrooms. in Molecules. 2015;20(10):19489-19525.
doi:10.3390/molecules201019489 .

Kozarski, Maja S., Klaus, Anita, Jakovljević, Dragica, Todorović, Nina, Vunduk, Jovana, Petrović, Predrag, Nikšić, Miomir, Vrvić, Miroslav, Van Griensven, Leo J. L. D., "Antioxidants of Edible Mushrooms" in Molecules, 20, no. 10 (2015):19489-19525,
https://doi.org/10.3390/molecules201019489 . .