TY  - JOUR
AU  - Jovanovic, Jovana
AU  - Kolarevic, Stoimir
AU  - Miloskovic, Aleksandra
AU  - Radojkovic, Natasa
AU  - Simić, Vladica
AU  - Dojčinović, Biljana
AU  - Kracun-Kolarevic, Margareta
AU  - Paunović, Momir
AU  - Kostic, Jovana
AU  - Sunjog, Karolina
AU  - Timilijic, Jovana
AU  - Đorđević, Jelena B.
AU  - Gačić, Zoran
AU  - Zegura, Bojana
AU  - Vukovic-Gacic, Branka
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2468
AB  - The Velika Morava River is the greatest national Serbian river and the significant tributary of the Danube River. The major problems in the Velika Morava River Basin (VMRB) represent untreated industrial and municipal wastewaters. In this study, the level of genotoxic potential at the sites along the VMRB was evaluated by parallel in vitro and in situ approach. Within in vitro testing, genotoxicity of native water samples collected from the sites in VMRB was evaluated by SOS/umuC test on Salmonella typhimurium TA1535/pSK1002 and by the comet assay on HepG2 cells. DNA damage in situ was assessed in bleak (Alburnus alburnus) erythrocytes by the comet (alkaline and Fpg-modified comet) and micronucleus assays. Additionally, the concentration of heavy metals in fish tissue was measured and this data, compiled with the data of the physico-chemical parameters measured in water, was used as a measure of the pollution pressure at the sites. Results showed that applied in vitro tests with native water samples are less sensitive in comparison with in situ tests and should be taken with precaution when making predictions on the status of the ecosystem. Within applied battery of in situ assays differential sensitivity of assays was observed where alkaline comet assay showed the highest potential in differentiation of the sites based on genotoxic potential. Integrated biomarker response showed that usage of the battery of bioassays provides better insight in a genotoxic effects in animals, and consequently, that the holistic approach is more suitable for this type of study.
PB  - Elsevier
T2  - Science of the Total Environment
T1  - Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ
VL  - 621
SP  - 1289
EP  - 1299
DO  - 10.1016/j.scitotenv.2017.10.099
ER  - 

@article{
author = "Jovanovic, Jovana and Kolarevic, Stoimir and Miloskovic, Aleksandra and Radojkovic, Natasa and Simić, Vladica and Dojčinović, Biljana and Kracun-Kolarevic, Margareta and Paunović, Momir and Kostic, Jovana and Sunjog, Karolina and Timilijic, Jovana and Đorđević, Jelena B. and Gačić, Zoran and Zegura, Bojana and Vukovic-Gacic, Branka",
year = "2018",
abstract = "The Velika Morava River is the greatest national Serbian river and the significant tributary of the Danube River. The major problems in the Velika Morava River Basin (VMRB) represent untreated industrial and municipal wastewaters. In this study, the level of genotoxic potential at the sites along the VMRB was evaluated by parallel in vitro and in situ approach. Within in vitro testing, genotoxicity of native water samples collected from the sites in VMRB was evaluated by SOS/umuC test on Salmonella typhimurium TA1535/pSK1002 and by the comet assay on HepG2 cells. DNA damage in situ was assessed in bleak (Alburnus alburnus) erythrocytes by the comet (alkaline and Fpg-modified comet) and micronucleus assays. Additionally, the concentration of heavy metals in fish tissue was measured and this data, compiled with the data of the physico-chemical parameters measured in water, was used as a measure of the pollution pressure at the sites. Results showed that applied in vitro tests with native water samples are less sensitive in comparison with in situ tests and should be taken with precaution when making predictions on the status of the ecosystem. Within applied battery of in situ assays differential sensitivity of assays was observed where alkaline comet assay showed the highest potential in differentiation of the sites based on genotoxic potential. Integrated biomarker response showed that usage of the battery of bioassays provides better insight in a genotoxic effects in animals, and consequently, that the holistic approach is more suitable for this type of study.",
publisher = "Elsevier",
journal = "Science of the Total Environment",
title = "Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ",
volume = "621",
pages = "1289-1299",
doi = "10.1016/j.scitotenv.2017.10.099"
}

Jovanovic, J., Kolarevic, S., Miloskovic, A., Radojkovic, N., Simić, V., Dojčinović, B., Kracun-Kolarevic, M., Paunović, M., Kostic, J., Sunjog, K., Timilijic, J., Đorđević, J. B., Gačić, Z., Zegura, B.,& Vukovic-Gacic, B.. (2018). Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ. in Science of the Total Environment
Elsevier., 621, 1289-1299.
https://doi.org/10.1016/j.scitotenv.2017.10.099

Jovanovic J, Kolarevic S, Miloskovic A, Radojkovic N, Simić V, Dojčinović B, Kracun-Kolarevic M, Paunović M, Kostic J, Sunjog K, Timilijic J, Đorđević JB, Gačić Z, Zegura B, Vukovic-Gacic B. Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ. in Science of the Total Environment. 2018;621:1289-1299.
doi:10.1016/j.scitotenv.2017.10.099 .

Jovanovic, Jovana, Kolarevic, Stoimir, Miloskovic, Aleksandra, Radojkovic, Natasa, Simić, Vladica, Dojčinović, Biljana, Kracun-Kolarevic, Margareta, Paunović, Momir, Kostic, Jovana, Sunjog, Karolina, Timilijic, Jovana, Đorđević, Jelena B., Gačić, Zoran, Zegura, Bojana, Vukovic-Gacic, Branka, "Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ" in Science of the Total Environment, 621 (2018):1289-1299,
https://doi.org/10.1016/j.scitotenv.2017.10.099 . .

TY  - JOUR
AU  - Marin, Marija
AU  - Novaković, Miroslav
AU  - Vučković, Ivan
AU  - Tešević, Vele
AU  - Kolarevic, Stoimir
AU  - Vukovic-Gacic, Branka
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2314
AB  - The composition of the essential oil (EO) obtained by hydrodistillation from the leaves of Thymus capitatus Hoff. et Link. growing wild in Sythonia (Greece) was analysed by GC-FID, GC-MS and quantitative H-1 NMR spectroscopy. Thirty nine compounds were identified, representing approximately 99.0 % of the oil. Major component determined by GC-FID was carvacrol (81.8 %), followed by linalool (3.5 %) and (E)-caryophyllenc (3.5 %). Abundance of carvacrol determined by quantitative H-1 NMR spectroscopy was 74.9 % and was in reasonable agreement with GC-FID result. The oil was tested for the antioxidant activity in DPPH assay and showed a dose dependent free radical scavenging activity with EC50 value of 1.03 mu g/ml. Antimicrobial effect of the EO was tested against Gram negative, Gram positive bacteria, and fungi. The tested EO showed significant activity against fungi, especially S. cerevisiae. The most sensitive bacteria were E. coil ATCC 25922, E. fecalis and S. epidermidis. Human pathogen Burkholderia cepacia, ATCC 25416 was used in the investigation of T capitatus for the fast time.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Journal of Essential Oil Bearing Plants
T1  - Wild Thymus capitatus Hoff. Et Link. Chemical Composition, Antioxidant and Antimicrobial Activities of the Essential Oil
VL  - 21
IS  - 2
SP  - 388
EP  - 399
DO  - 10.1080/0972060X.2018.1449668
ER  - 

@article{
author = "Marin, Marija and Novaković, Miroslav and Vučković, Ivan and Tešević, Vele and Kolarevic, Stoimir and Vukovic-Gacic, Branka",
year = "2018",
abstract = "The composition of the essential oil (EO) obtained by hydrodistillation from the leaves of Thymus capitatus Hoff. et Link. growing wild in Sythonia (Greece) was analysed by GC-FID, GC-MS and quantitative H-1 NMR spectroscopy. Thirty nine compounds were identified, representing approximately 99.0 % of the oil. Major component determined by GC-FID was carvacrol (81.8 %), followed by linalool (3.5 %) and (E)-caryophyllenc (3.5 %). Abundance of carvacrol determined by quantitative H-1 NMR spectroscopy was 74.9 % and was in reasonable agreement with GC-FID result. The oil was tested for the antioxidant activity in DPPH assay and showed a dose dependent free radical scavenging activity with EC50 value of 1.03 mu g/ml. Antimicrobial effect of the EO was tested against Gram negative, Gram positive bacteria, and fungi. The tested EO showed significant activity against fungi, especially S. cerevisiae. The most sensitive bacteria were E. coil ATCC 25922, E. fecalis and S. epidermidis. Human pathogen Burkholderia cepacia, ATCC 25416 was used in the investigation of T capitatus for the fast time.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Journal of Essential Oil Bearing Plants",
title = "Wild Thymus capitatus Hoff. Et Link. Chemical Composition, Antioxidant and Antimicrobial Activities of the Essential Oil",
volume = "21",
number = "2",
pages = "388-399",
doi = "10.1080/0972060X.2018.1449668"
}

Marin, M., Novaković, M., Vučković, I., Tešević, V., Kolarevic, S.,& Vukovic-Gacic, B.. (2018). Wild Thymus capitatus Hoff. Et Link. Chemical Composition, Antioxidant and Antimicrobial Activities of the Essential Oil. in Journal of Essential Oil Bearing Plants
Taylor & Francis Ltd, Abingdon., 21(2), 388-399.
https://doi.org/10.1080/0972060X.2018.1449668

Marin M, Novaković M, Vučković I, Tešević V, Kolarevic S, Vukovic-Gacic B. Wild Thymus capitatus Hoff. Et Link. Chemical Composition, Antioxidant and Antimicrobial Activities of the Essential Oil. in Journal of Essential Oil Bearing Plants. 2018;21(2):388-399.
doi:10.1080/0972060X.2018.1449668 .

Marin, Marija, Novaković, Miroslav, Vučković, Ivan, Tešević, Vele, Kolarevic, Stoimir, Vukovic-Gacic, Branka, "Wild Thymus capitatus Hoff. Et Link. Chemical Composition, Antioxidant and Antimicrobial Activities of the Essential Oil" in Journal of Essential Oil Bearing Plants, 21, no. 2 (2018):388-399,
https://doi.org/10.1080/0972060X.2018.1449668 . .

TY  - JOUR
AU  - Marin, Marija
AU  - Novaković, Miroslav
AU  - Tešević, Vele
AU  - Kolarevic, Stoimir
AU  - Vukovic-Gacic, Branka
PY  - 2018
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2378
AB  - In this study, we compared the compositions, antimicrobial and potential antioxidant activities of essential oils of two Thymus endemic, wild-growing species - T. malyi Ronninger and T. lykae Degen et Jay. No information about its composition and biological activities of oils has been reported to the present day. Forty three compounds were identified from T. malyi and major component was a-pinene (26.4%). In the case of T. lykae, forty four compounds were identified, with geranyl acetate (35.1%) as the main component. Antioxidant activities of the oils were evaluated using DPPH assay. The antimicrobial effect of essential oils were tested against Gram-negative, Gram-positive bacteria, and two fungi. Results indicated higher antibacterial activity of the essential oils of T. lykae in comparison with T. malyi, while for antifungal activity, it was reverse. The essential oils from T. malyi and T. lykae possess antioxidant and antimicrobial activities and could be used as a potential source of natural antioxidants for the food industry.
PB  - Wydawnictwo Akad Rolniczej W Lublinie, Lublin
T2  - Acta Scientiarum Polonorum-Hortorum Cultus
T1  - Comparision of the compositions, antimicrobial and antioxidant activities of essential oils from the endemic species thymus malyi ronninger and thymus lykae degen et jav.
VL  - 17
IS  - 5
SP  - 157
EP  - 165
DO  - 10.24326/asphc.2018.5.14
ER  - 

@article{
author = "Marin, Marija and Novaković, Miroslav and Tešević, Vele and Kolarevic, Stoimir and Vukovic-Gacic, Branka",
year = "2018",
abstract = "In this study, we compared the compositions, antimicrobial and potential antioxidant activities of essential oils of two Thymus endemic, wild-growing species - T. malyi Ronninger and T. lykae Degen et Jay. No information about its composition and biological activities of oils has been reported to the present day. Forty three compounds were identified from T. malyi and major component was a-pinene (26.4%). In the case of T. lykae, forty four compounds were identified, with geranyl acetate (35.1%) as the main component. Antioxidant activities of the oils were evaluated using DPPH assay. The antimicrobial effect of essential oils were tested against Gram-negative, Gram-positive bacteria, and two fungi. Results indicated higher antibacterial activity of the essential oils of T. lykae in comparison with T. malyi, while for antifungal activity, it was reverse. The essential oils from T. malyi and T. lykae possess antioxidant and antimicrobial activities and could be used as a potential source of natural antioxidants for the food industry.",
publisher = "Wydawnictwo Akad Rolniczej W Lublinie, Lublin",
journal = "Acta Scientiarum Polonorum-Hortorum Cultus",
title = "Comparision of the compositions, antimicrobial and antioxidant activities of essential oils from the endemic species thymus malyi ronninger and thymus lykae degen et jav.",
volume = "17",
number = "5",
pages = "157-165",
doi = "10.24326/asphc.2018.5.14"
}

Marin, M., Novaković, M., Tešević, V., Kolarevic, S.,& Vukovic-Gacic, B.. (2018). Comparision of the compositions, antimicrobial and antioxidant activities of essential oils from the endemic species thymus malyi ronninger and thymus lykae degen et jav.. in Acta Scientiarum Polonorum-Hortorum Cultus
Wydawnictwo Akad Rolniczej W Lublinie, Lublin., 17(5), 157-165.
https://doi.org/10.24326/asphc.2018.5.14

Marin M, Novaković M, Tešević V, Kolarevic S, Vukovic-Gacic B. Comparision of the compositions, antimicrobial and antioxidant activities of essential oils from the endemic species thymus malyi ronninger and thymus lykae degen et jav.. in Acta Scientiarum Polonorum-Hortorum Cultus. 2018;17(5):157-165.
doi:10.24326/asphc.2018.5.14 .

Marin, Marija, Novaković, Miroslav, Tešević, Vele, Kolarevic, Stoimir, Vukovic-Gacic, Branka, "Comparision of the compositions, antimicrobial and antioxidant activities of essential oils from the endemic species thymus malyi ronninger and thymus lykae degen et jav." in Acta Scientiarum Polonorum-Hortorum Cultus, 17, no. 5 (2018):157-165,
https://doi.org/10.24326/asphc.2018.5.14 . .

TY  - JOUR
AU  - Kolarevic, Stoimir
AU  - Aborgiba, Mustafa
AU  - Kracun-Kolarevic, Margareta
AU  - Kostic, Jovana
AU  - Simonović, Predrag
AU  - Simić, Vladica
AU  - Miloskovic, Aleksandra
AU  - Reischer, Georg
AU  - Farnleitner, Andreas
AU  - Gačić, Zoran
AU  - Milacic, Radmila
AU  - Zuliani, Tea
AU  - Vidmar, Janja
AU  - Pergal, Marija
AU  - Piria, Marina
AU  - Paunović, Momir
AU  - Vukovic-Gacic, Branka
PY  - 2016
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1920
AB  - In this study we have performed a comprehensive genotoxicological survey along the 900 rkm of the Sava River. In total, 12 sites were chosen in compliance with the goals of GLOBAQUA project dealing with the effects of multiple stressors on biodiversity and functioning of aquatic ecosystems. The genotoxic potential was assessed using a complex battery of bioassays performed in prokaryotes and aquatic eukaryotes (freshwater fish). Battery comprised evaluation of mutagenicity by SOS/umuC test in Salmonella typhimurium TA1535/pSK1002. The level of DNA damage as a biomarker of exposure (comet assay) and biomarker of effect (micronucleus assay) and the level of oxidative stress as well (Fpg-modified comet assay) was studied in blood cells of bleak and spirlin (Alburnus alburnus/Alburnoides bipunctatus respectively). Result indicated differential sensitivity of applied bioassays in detection of genotoxic pressure. The standard and Fpg-modified comet assay showed higher potential in differentiation of the sites based on genotoxic potential in comparison with micronucleus assay and SOS/umuC test. Our data represent snapshot of the current status of the river which indicates the presence of genotoxic potential along the river which can be traced to the deterioration of quality of the Sava River by communal and industrial wastewaters. The major highlight of the study is that we have provided complex set of data obtained from a single source (homogeneity of analyses for all samples).
PB  - Public Library of Science (PLoS)
T2  - Plos One
T1  - Evaluation of Genotoxic Pressure along the Sava River
VL  - 11
IS  - 9
DO  - 10.1371/journal.pone.0162450
ER  - 

@article{
author = "Kolarevic, Stoimir and Aborgiba, Mustafa and Kracun-Kolarevic, Margareta and Kostic, Jovana and Simonović, Predrag and Simić, Vladica and Miloskovic, Aleksandra and Reischer, Georg and Farnleitner, Andreas and Gačić, Zoran and Milacic, Radmila and Zuliani, Tea and Vidmar, Janja and Pergal, Marija and Piria, Marina and Paunović, Momir and Vukovic-Gacic, Branka",
year = "2016",
abstract = "In this study we have performed a comprehensive genotoxicological survey along the 900 rkm of the Sava River. In total, 12 sites were chosen in compliance with the goals of GLOBAQUA project dealing with the effects of multiple stressors on biodiversity and functioning of aquatic ecosystems. The genotoxic potential was assessed using a complex battery of bioassays performed in prokaryotes and aquatic eukaryotes (freshwater fish). Battery comprised evaluation of mutagenicity by SOS/umuC test in Salmonella typhimurium TA1535/pSK1002. The level of DNA damage as a biomarker of exposure (comet assay) and biomarker of effect (micronucleus assay) and the level of oxidative stress as well (Fpg-modified comet assay) was studied in blood cells of bleak and spirlin (Alburnus alburnus/Alburnoides bipunctatus respectively). Result indicated differential sensitivity of applied bioassays in detection of genotoxic pressure. The standard and Fpg-modified comet assay showed higher potential in differentiation of the sites based on genotoxic potential in comparison with micronucleus assay and SOS/umuC test. Our data represent snapshot of the current status of the river which indicates the presence of genotoxic potential along the river which can be traced to the deterioration of quality of the Sava River by communal and industrial wastewaters. The major highlight of the study is that we have provided complex set of data obtained from a single source (homogeneity of analyses for all samples).",
publisher = "Public Library of Science (PLoS)",
journal = "Plos One",
title = "Evaluation of Genotoxic Pressure along the Sava River",
volume = "11",
number = "9",
doi = "10.1371/journal.pone.0162450"
}

Kolarevic, S., Aborgiba, M., Kracun-Kolarevic, M., Kostic, J., Simonović, P., Simić, V., Miloskovic, A., Reischer, G., Farnleitner, A., Gačić, Z., Milacic, R., Zuliani, T., Vidmar, J., Pergal, M., Piria, M., Paunović, M.,& Vukovic-Gacic, B.. (2016). Evaluation of Genotoxic Pressure along the Sava River. in Plos One
Public Library of Science (PLoS)., 11(9).
https://doi.org/10.1371/journal.pone.0162450

Kolarevic S, Aborgiba M, Kracun-Kolarevic M, Kostic J, Simonović P, Simić V, Miloskovic A, Reischer G, Farnleitner A, Gačić Z, Milacic R, Zuliani T, Vidmar J, Pergal M, Piria M, Paunović M, Vukovic-Gacic B. Evaluation of Genotoxic Pressure along the Sava River. in Plos One. 2016;11(9).
doi:10.1371/journal.pone.0162450 .

Kolarevic, Stoimir, Aborgiba, Mustafa, Kracun-Kolarevic, Margareta, Kostic, Jovana, Simonović, Predrag, Simić, Vladica, Miloskovic, Aleksandra, Reischer, Georg, Farnleitner, Andreas, Gačić, Zoran, Milacic, Radmila, Zuliani, Tea, Vidmar, Janja, Pergal, Marija, Piria, Marina, Paunović, Momir, Vukovic-Gacic, Branka, "Evaluation of Genotoxic Pressure along the Sava River" in Plos One, 11, no. 9 (2016),
https://doi.org/10.1371/journal.pone.0162450 . .