TY  - JOUR
AU  - Gorjanović, Stanislava
AU  - Pastor, Ferenc T.
AU  - Vasic, Radica
AU  - Novaković, Miroslav
AU  - Simonović, Mladen
AU  - Milić, Sonja
AU  - Suznjevic, Desanka
PY  - 2013
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1239
AB  - Antioxidant (AO) activity of extracts of hop cones (Serbian domestic varieties) and commercial hop products (Saaz, Spalter, Spalter select, and Magnum pellets) was determined by parallel application of recently developed direct current (DC) polarographic and widely used DPPH assay. Correlations between 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) scavenging and total phenolics (TPC) determined by the Folin-Ciocalteu assay (FC) (0.99), and between H2O2 scavenging,: (HPS) and humulone content (H) determined by conductometric method (0.94), total resins (TR) (0.85), and hop storage index (HIS) (-0.90), were found statistically significant at p  LT  0.05 level while complete lack of HPS correlation with TPC and DPPH was observed. To obtain an insight into differences between results of AO assays applied, activity of individual compounds, prevalent hop phenolics, and bitter acids was determined. By far superior HPS activity of humulone was followed by catechin, quercetin, xanthohumol, lupulone, and rutin. In contrast, DPPH scavenging activity of phenolics (quercetin > catechin > rutin > xantohumol) was found substantially higher than activity of bitter acids. According to ferric reducing antioxidant power (FRAP) and scavenging of 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), higher AO activity was ascribed to phenolics, while almost neglecting humulone. Besides reliability, low cost, and an easy-to-handle procedure, an ability to recognize humulone as the major contributor of hop AO activity could allow DC polarographic assay to be applied in analysis of various hop-derived products.
PB  - American Chemical Society (ACS)
T2  - Journal of Agricultural and Food Chemistry
T1  - Electrochemical versus Spectrophotometric Assessment of Antioxidant Activity of Hop (Humulus lupulus L.) Products and Individual Compounds
VL  - 61
IS  - 38
SP  - 9089
EP  - 9096
DO  - 10.1021/jf401718z
ER  - 

@article{
author = "Gorjanović, Stanislava and Pastor, Ferenc T. and Vasic, Radica and Novaković, Miroslav and Simonović, Mladen and Milić, Sonja and Suznjevic, Desanka",
year = "2013",
abstract = "Antioxidant (AO) activity of extracts of hop cones (Serbian domestic varieties) and commercial hop products (Saaz, Spalter, Spalter select, and Magnum pellets) was determined by parallel application of recently developed direct current (DC) polarographic and widely used DPPH assay. Correlations between 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) scavenging and total phenolics (TPC) determined by the Folin-Ciocalteu assay (FC) (0.99), and between H2O2 scavenging,: (HPS) and humulone content (H) determined by conductometric method (0.94), total resins (TR) (0.85), and hop storage index (HIS) (-0.90), were found statistically significant at p  LT  0.05 level while complete lack of HPS correlation with TPC and DPPH was observed. To obtain an insight into differences between results of AO assays applied, activity of individual compounds, prevalent hop phenolics, and bitter acids was determined. By far superior HPS activity of humulone was followed by catechin, quercetin, xanthohumol, lupulone, and rutin. In contrast, DPPH scavenging activity of phenolics (quercetin > catechin > rutin > xantohumol) was found substantially higher than activity of bitter acids. According to ferric reducing antioxidant power (FRAP) and scavenging of 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), higher AO activity was ascribed to phenolics, while almost neglecting humulone. Besides reliability, low cost, and an easy-to-handle procedure, an ability to recognize humulone as the major contributor of hop AO activity could allow DC polarographic assay to be applied in analysis of various hop-derived products.",
publisher = "American Chemical Society (ACS)",
journal = "Journal of Agricultural and Food Chemistry",
title = "Electrochemical versus Spectrophotometric Assessment of Antioxidant Activity of Hop (Humulus lupulus L.) Products and Individual Compounds",
volume = "61",
number = "38",
pages = "9089-9096",
doi = "10.1021/jf401718z"
}

Gorjanović, S., Pastor, F. T., Vasic, R., Novaković, M., Simonović, M., Milić, S.,& Suznjevic, D.. (2013). Electrochemical versus Spectrophotometric Assessment of Antioxidant Activity of Hop (Humulus lupulus L.) Products and Individual Compounds. in Journal of Agricultural and Food Chemistry
American Chemical Society (ACS)., 61(38), 9089-9096.
https://doi.org/10.1021/jf401718z

Gorjanović S, Pastor FT, Vasic R, Novaković M, Simonović M, Milić S, Suznjevic D. Electrochemical versus Spectrophotometric Assessment of Antioxidant Activity of Hop (Humulus lupulus L.) Products and Individual Compounds. in Journal of Agricultural and Food Chemistry. 2013;61(38):9089-9096.
doi:10.1021/jf401718z .

Gorjanović, Stanislava, Pastor, Ferenc T., Vasic, Radica, Novaković, Miroslav, Simonović, Mladen, Milić, Sonja, Suznjevic, Desanka, "Electrochemical versus Spectrophotometric Assessment of Antioxidant Activity of Hop (Humulus lupulus L.) Products and Individual Compounds" in Journal of Agricultural and Food Chemistry, 61, no. 38 (2013):9089-9096,
https://doi.org/10.1021/jf401718z . .

TY  - JOUR
AU  - Matić, Ivana Z.
AU  - Žižak, Željko
AU  - Simonović, Mladen
AU  - Simonović, Branislav
AU  - Gođevac, Dejan
AU  - Savikin, Katarina
AU  - Juranić, Zorica
PY  - 2010
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/697
AB  - Red and white wine polyphenols have been reported to provide substantial health benefits. In this study, the cytotoxic activity of red and white wine polyphenolic extracts and of resveratrol was evaluated against different cancer cell lines-human cervix adenocarcinoma HeLa, human breast adenocarcinoma MDA-MB-361, and human breast carcinoma MDA-MB-453 and normal human peripheral blood mononuclear cells (PBMCs). Qualitative and quantitative compositions of wine polyphenolic extracts obtained by fractional vacuum distillation of corresponding wines were determined using spectrophotometric methods and high-performance liquid chromatography with diode array detection and liquid chromatography with electrospray ionization-time of flight mass spectrometry analysis. It was demonstrated that wine polyphenolic extracts and resveratrol exerted higher cytotoxic activity against HeLa and MDA-MB-453 cells in comparison to MDA-MB-361 cells and unstimulated and stimulated PBMCs. Furthermore, white wine polyphenolic extract exhibited a significantly higher antiproliferative action on cancer cell lines than red wine extract. The presence of condensed or fragmented nuclei in HeLa cells, pretreated with extract of white wine and stained with a mixture of acridine orange and ethidium bromide, pointed to the morphological signs of apoptosis. In addition, HeLa cells in late stages of apoptosis or secondary necrosis were also observed. Results from our study suggest that polyphenolic extracts from red and white wine may have anticarcinogenic potential.
PB  - Mary Ann Liebert Inc, New Rochelle
T2  - Journal of Medicinal Food
T1  - Cytotoxic Effect of Wine Polyphenolic Extracts and Resveratrol Against Human Carcinoma Cells and Normal Peripheral Blood Mononuclear Cells
VL  - 13
IS  - 4
SP  - 851
EP  - 862
DO  - 10.1089/jmf.2009.0193
ER  - 

@article{
author = "Matić, Ivana Z. and Žižak, Željko and Simonović, Mladen and Simonović, Branislav and Gođevac, Dejan and Savikin, Katarina and Juranić, Zorica",
year = "2010",
abstract = "Red and white wine polyphenols have been reported to provide substantial health benefits. In this study, the cytotoxic activity of red and white wine polyphenolic extracts and of resveratrol was evaluated against different cancer cell lines-human cervix adenocarcinoma HeLa, human breast adenocarcinoma MDA-MB-361, and human breast carcinoma MDA-MB-453 and normal human peripheral blood mononuclear cells (PBMCs). Qualitative and quantitative compositions of wine polyphenolic extracts obtained by fractional vacuum distillation of corresponding wines were determined using spectrophotometric methods and high-performance liquid chromatography with diode array detection and liquid chromatography with electrospray ionization-time of flight mass spectrometry analysis. It was demonstrated that wine polyphenolic extracts and resveratrol exerted higher cytotoxic activity against HeLa and MDA-MB-453 cells in comparison to MDA-MB-361 cells and unstimulated and stimulated PBMCs. Furthermore, white wine polyphenolic extract exhibited a significantly higher antiproliferative action on cancer cell lines than red wine extract. The presence of condensed or fragmented nuclei in HeLa cells, pretreated with extract of white wine and stained with a mixture of acridine orange and ethidium bromide, pointed to the morphological signs of apoptosis. In addition, HeLa cells in late stages of apoptosis or secondary necrosis were also observed. Results from our study suggest that polyphenolic extracts from red and white wine may have anticarcinogenic potential.",
publisher = "Mary Ann Liebert Inc, New Rochelle",
journal = "Journal of Medicinal Food",
title = "Cytotoxic Effect of Wine Polyphenolic Extracts and Resveratrol Against Human Carcinoma Cells and Normal Peripheral Blood Mononuclear Cells",
volume = "13",
number = "4",
pages = "851-862",
doi = "10.1089/jmf.2009.0193"
}

Matić, I. Z., Žižak, Ž., Simonović, M., Simonović, B., Gođevac, D., Savikin, K.,& Juranić, Z.. (2010). Cytotoxic Effect of Wine Polyphenolic Extracts and Resveratrol Against Human Carcinoma Cells and Normal Peripheral Blood Mononuclear Cells. in Journal of Medicinal Food
Mary Ann Liebert Inc, New Rochelle., 13(4), 851-862.
https://doi.org/10.1089/jmf.2009.0193

Matić IZ, Žižak Ž, Simonović M, Simonović B, Gođevac D, Savikin K, Juranić Z. Cytotoxic Effect of Wine Polyphenolic Extracts and Resveratrol Against Human Carcinoma Cells and Normal Peripheral Blood Mononuclear Cells. in Journal of Medicinal Food. 2010;13(4):851-862.
doi:10.1089/jmf.2009.0193 .

Matić, Ivana Z., Žižak, Željko, Simonović, Mladen, Simonović, Branislav, Gođevac, Dejan, Savikin, Katarina, Juranić, Zorica, "Cytotoxic Effect of Wine Polyphenolic Extracts and Resveratrol Against Human Carcinoma Cells and Normal Peripheral Blood Mononuclear Cells" in Journal of Medicinal Food, 13, no. 4 (2010):851-862,
https://doi.org/10.1089/jmf.2009.0193 . .

TY  - JOUR
AU  - Simonović, Mladen
AU  - Milošević-Zlatanović, Svetlana
AU  - Milosavić, Nenad
AU  - Vrvić, Miroslav
AU  - Simonović, Branislav
PY  - 2009
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3414
AB  - Diverse recombinant immunoreagents specific for TNT–derivatives were tested in different assay forms in order to analyze their specificity and sensitivity. Performance of immunoassays was based on TNP–protein conjugates immobilization on a solid surface. In this work, the detection limit for TNT–analog TNP–Tris was 250 fmol or 87 pg mL−1 (87 ppt), which represents the most sensitive assay published until now, regarding the detection of recombinant antibodies.
PB  - Versita
T2  - Chemical Papers
T1  - Characterization of recombinant antibodies for detection of TNT and its derivatives
VL  - 63
IS  - 4
SP  - 391
EP  - 398
DO  - 10.2478/s11696-009-0043-5
ER  - 

@article{
author = "Simonović, Mladen and Milošević-Zlatanović, Svetlana and Milosavić, Nenad and Vrvić, Miroslav and Simonović, Branislav",
year = "2009",
abstract = "Diverse recombinant immunoreagents specific for TNT–derivatives were tested in different assay forms in order to analyze their specificity and sensitivity. Performance of immunoassays was based on TNP–protein conjugates immobilization on a solid surface. In this work, the detection limit for TNT–analog TNP–Tris was 250 fmol or 87 pg mL−1 (87 ppt), which represents the most sensitive assay published until now, regarding the detection of recombinant antibodies.",
publisher = "Versita",
journal = "Chemical Papers",
title = "Characterization of recombinant antibodies for detection of TNT and its derivatives",
volume = "63",
number = "4",
pages = "391-398",
doi = "10.2478/s11696-009-0043-5"
}

Simonović, M., Milošević-Zlatanović, S., Milosavić, N., Vrvić, M.,& Simonović, B.. (2009). Characterization of recombinant antibodies for detection of TNT and its derivatives. in Chemical Papers
Versita., 63(4), 391-398.
https://doi.org/10.2478/s11696-009-0043-5

Simonović M, Milošević-Zlatanović S, Milosavić N, Vrvić M, Simonović B. Characterization of recombinant antibodies for detection of TNT and its derivatives. in Chemical Papers. 2009;63(4):391-398.
doi:10.2478/s11696-009-0043-5 .

Simonović, Mladen, Milošević-Zlatanović, Svetlana, Milosavić, Nenad, Vrvić, Miroslav, Simonović, Branislav, "Characterization of recombinant antibodies for detection of TNT and its derivatives" in Chemical Papers, 63, no. 4 (2009):391-398,
https://doi.org/10.2478/s11696-009-0043-5 . .