TY  - JOUR
AU  - Aćimović, Milica
AU  - Stanković Jeremić, Jovana
AU  - Miljković, Ana
AU  - Rat, Milica
AU  - Lončar, Biljana
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/7200
AB  - Ironwort (Sideritis montana L.), mountain germander (Teucrium montanum L.), wall germander (Teucrium chamaedrys L.), and horehound (Marrubium peregrinum L.) are species widely distributedacross Europe and are also found in North Africa and West Asia. Because of their wide distributionthey express significant chemical diversity. For generations, these plants have been used as medicalherbs for treating different aliments. The aim of this paper is to analyze volatile compounds of fourselected species that belong to the subfamily Lamioideae, family Lamiaceae, and inspect scientificallyproven biological activities and potential uses in modern phytotherapy in relation to traditionalmedicine. Therefore, in this research, we analyze the volatile compounds from this plants, obtainedin laboratory by a Clevenger-type apparatus, followed by liquid–liquid extraction with hexane as thesolvent. The identification of volatile compounds is conducted by GC-FID and GC-MS. Althoughthese plants are poor in essential oil, the most abundant class of volatile components are mainlysesquiterpenes: germacrene D (22.6%) in ironwort, 7-epi-trans-sesquisabinene hydrate (15.8%) inmountain germander, germacrene D (31.8%) and trans-caryophyllene (19.7%) in wall germander, andtrans-caryophyllene (32.4%) and trans-thujone (25.1%) in horehound. Furthermore, many studiesshow that, in addition to the essential oil, these plants contain phenols, flavonoids, diterpenes andditerpenoids, iridoids and their glycosides, coumarins, terpenes, and sterols, among other activecompounds, which affect biological activities. The other goal of this study is to review the literaturethat describes the traditional use of these plants in folk medicine in regions where they grow spontaneously and compare them with scientifically confirmed activities. Therefore, a bibliographic searchis conducted on Science Direct, PubMed, and Google Scholar to gather information related to thetopic and recommend potential applications in modern phytotherapy. In conclusion, we can say thatselected plants could be used as natural agents for promoting health, as a source of raw materialin the food industry, and as supplements, as well as in the pharmaceutical industry for developingplant-based remedies for prevention and treatment of many diseases, especially cancer.
PB  - Basel : MDPI
T2  - Molecules - Basel
T1  - Screening of Volatile Compounds, Traditional and Modern Phytotherapy Approaches of Selected Non-Aromatic Medicinal Plants (Lamiaceae, Lamioideae) from Rtanj Mountain, Eastern Serbia
VL  - 28
IS  - 12
SP  - 4611
DO  - 10.3390/molecules28124611
ER  - 

@article{
author = "Aćimović, Milica and Stanković Jeremić, Jovana and Miljković, Ana and Rat, Milica and Lončar, Biljana",
year = "2023",
abstract = "Ironwort (Sideritis montana L.), mountain germander (Teucrium montanum L.), wall germander (Teucrium chamaedrys L.), and horehound (Marrubium peregrinum L.) are species widely distributedacross Europe and are also found in North Africa and West Asia. Because of their wide distributionthey express significant chemical diversity. For generations, these plants have been used as medicalherbs for treating different aliments. The aim of this paper is to analyze volatile compounds of fourselected species that belong to the subfamily Lamioideae, family Lamiaceae, and inspect scientificallyproven biological activities and potential uses in modern phytotherapy in relation to traditionalmedicine. Therefore, in this research, we analyze the volatile compounds from this plants, obtainedin laboratory by a Clevenger-type apparatus, followed by liquid–liquid extraction with hexane as thesolvent. The identification of volatile compounds is conducted by GC-FID and GC-MS. Althoughthese plants are poor in essential oil, the most abundant class of volatile components are mainlysesquiterpenes: germacrene D (22.6%) in ironwort, 7-epi-trans-sesquisabinene hydrate (15.8%) inmountain germander, germacrene D (31.8%) and trans-caryophyllene (19.7%) in wall germander, andtrans-caryophyllene (32.4%) and trans-thujone (25.1%) in horehound. Furthermore, many studiesshow that, in addition to the essential oil, these plants contain phenols, flavonoids, diterpenes andditerpenoids, iridoids and their glycosides, coumarins, terpenes, and sterols, among other activecompounds, which affect biological activities. The other goal of this study is to review the literaturethat describes the traditional use of these plants in folk medicine in regions where they grow spontaneously and compare them with scientifically confirmed activities. Therefore, a bibliographic searchis conducted on Science Direct, PubMed, and Google Scholar to gather information related to thetopic and recommend potential applications in modern phytotherapy. In conclusion, we can say thatselected plants could be used as natural agents for promoting health, as a source of raw materialin the food industry, and as supplements, as well as in the pharmaceutical industry for developingplant-based remedies for prevention and treatment of many diseases, especially cancer.",
publisher = "Basel : MDPI",
journal = "Molecules - Basel",
title = "Screening of Volatile Compounds, Traditional and Modern Phytotherapy Approaches of Selected Non-Aromatic Medicinal Plants (Lamiaceae, Lamioideae) from Rtanj Mountain, Eastern Serbia",
volume = "28",
number = "12",
pages = "4611",
doi = "10.3390/molecules28124611"
}

Aćimović, M., Stanković Jeremić, J., Miljković, A., Rat, M.,& Lončar, B.. (2023). Screening of Volatile Compounds, Traditional and Modern Phytotherapy Approaches of Selected Non-Aromatic Medicinal Plants (Lamiaceae, Lamioideae) from Rtanj Mountain, Eastern Serbia. in Molecules - Basel
Basel : MDPI., 28(12), 4611.
https://doi.org/10.3390/molecules28124611

Aćimović M, Stanković Jeremić J, Miljković A, Rat M, Lončar B. Screening of Volatile Compounds, Traditional and Modern Phytotherapy Approaches of Selected Non-Aromatic Medicinal Plants (Lamiaceae, Lamioideae) from Rtanj Mountain, Eastern Serbia. in Molecules - Basel. 2023;28(12):4611.
doi:10.3390/molecules28124611 .

Aćimović, Milica, Stanković Jeremić, Jovana, Miljković, Ana, Rat, Milica, Lončar, Biljana, "Screening of Volatile Compounds, Traditional and Modern Phytotherapy Approaches of Selected Non-Aromatic Medicinal Plants (Lamiaceae, Lamioideae) from Rtanj Mountain, Eastern Serbia" in Molecules - Basel, 28, no. 12 (2023):4611,
https://doi.org/10.3390/molecules28124611 . .

TY  - JOUR
AU  - Aćimović, Milica
AU  - Semerdijeva, Ivanka
AU  - Zheljazkov, Valtcho D.
AU  - Rat, Milica
AU  - Stanković Jeremić, Jovana
AU  - Lončar, Biljana
AU  - Vukić, Vladimir
AU  - Radovanović, Katarina
AU  - Gavarić, Neda
AU  - Pezo, Lato
PY  - 2023
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/6679
AB  - This research investigated a Balkan endemic species, Achillea clypeolata, collected from two locations (Dobrostan and Ivaylovgrad) in Bulgaria and two locations (Ozren and Rtanj) in Serbia. The goal was to compare the essential oil (EO) of A. clypeolata from these four localities. The EO was isolated from dry aboveground plant parts by hydrodistillation, and the identification of the EO constituents was carried out by GC-MS analyses. Principal Component Analysis (PCA), correlation analysis, and an unrooted cluster tree were used to define distinctions in the presence of volatile compounds in A. clypeolata EO. Results showed that A. clypeolata of Serbian origin (Ozren and Rtanj) contained 1,8-cineole as the main constituent, and belonged to the previously described 1,8-cineole as a dominant compound in the EO profile. On the other hand, the results from Bulgaria show an evident chemical diversification within the A. clypeolata species, as 1,8-cineole (23.2%), camphor (5.0%) and borneol (8.9%) prevailed in the EO of the studied samples collected from Ivaylovgrad, while borneol (22.6%), β-eudesmol (12.7%) and elemol (7.8%) were the main ones in the EO from Dobrostan. In sillico analysis revealed the high anti-inflammatory potential of A. clypeolata, which may be highly diverse depending on the population and active compounds it contains.
PB  - Elsevier BV
T2  - Biochemical Systematics and Ecology
T1  - Variation in the essential oil composition and in silico analysis of anti-inflammatory potential of Balkan endemic species Achillea clypeolata Sm
VL  - 110
SP  - 104679
DO  - 10.1016/j.bse.2023.104679
ER  - 

@article{
author = "Aćimović, Milica and Semerdijeva, Ivanka and Zheljazkov, Valtcho D. and Rat, Milica and Stanković Jeremić, Jovana and Lončar, Biljana and Vukić, Vladimir and Radovanović, Katarina and Gavarić, Neda and Pezo, Lato",
year = "2023",
abstract = "This research investigated a Balkan endemic species, Achillea clypeolata, collected from two locations (Dobrostan and Ivaylovgrad) in Bulgaria and two locations (Ozren and Rtanj) in Serbia. The goal was to compare the essential oil (EO) of A. clypeolata from these four localities. The EO was isolated from dry aboveground plant parts by hydrodistillation, and the identification of the EO constituents was carried out by GC-MS analyses. Principal Component Analysis (PCA), correlation analysis, and an unrooted cluster tree were used to define distinctions in the presence of volatile compounds in A. clypeolata EO. Results showed that A. clypeolata of Serbian origin (Ozren and Rtanj) contained 1,8-cineole as the main constituent, and belonged to the previously described 1,8-cineole as a dominant compound in the EO profile. On the other hand, the results from Bulgaria show an evident chemical diversification within the A. clypeolata species, as 1,8-cineole (23.2%), camphor (5.0%) and borneol (8.9%) prevailed in the EO of the studied samples collected from Ivaylovgrad, while borneol (22.6%), β-eudesmol (12.7%) and elemol (7.8%) were the main ones in the EO from Dobrostan. In sillico analysis revealed the high anti-inflammatory potential of A. clypeolata, which may be highly diverse depending on the population and active compounds it contains.",
publisher = "Elsevier BV",
journal = "Biochemical Systematics and Ecology",
title = "Variation in the essential oil composition and in silico analysis of anti-inflammatory potential of Balkan endemic species Achillea clypeolata Sm",
volume = "110",
pages = "104679",
doi = "10.1016/j.bse.2023.104679"
}

Aćimović, M., Semerdijeva, I., Zheljazkov, V. D., Rat, M., Stanković Jeremić, J., Lončar, B., Vukić, V., Radovanović, K., Gavarić, N.,& Pezo, L.. (2023). Variation in the essential oil composition and in silico analysis of anti-inflammatory potential of Balkan endemic species Achillea clypeolata Sm. in Biochemical Systematics and Ecology
Elsevier BV., 110, 104679.
https://doi.org/10.1016/j.bse.2023.104679

Aćimović M, Semerdijeva I, Zheljazkov VD, Rat M, Stanković Jeremić J, Lončar B, Vukić V, Radovanović K, Gavarić N, Pezo L. Variation in the essential oil composition and in silico analysis of anti-inflammatory potential of Balkan endemic species Achillea clypeolata Sm. in Biochemical Systematics and Ecology. 2023;110:104679.
doi:10.1016/j.bse.2023.104679 .

Aćimović, Milica, Semerdijeva, Ivanka, Zheljazkov, Valtcho D., Rat, Milica, Stanković Jeremić, Jovana, Lončar, Biljana, Vukić, Vladimir, Radovanović, Katarina, Gavarić, Neda, Pezo, Lato, "Variation in the essential oil composition and in silico analysis of anti-inflammatory potential of Balkan endemic species Achillea clypeolata Sm" in Biochemical Systematics and Ecology, 110 (2023):104679,
https://doi.org/10.1016/j.bse.2023.104679 . .

TY  - JOUR
AU  - Aćimović, Milica
AU  - Lončar, Biljana
AU  - Pezo, Milada
AU  - Stanković Jeremić, Jovana
AU  - Cvetković, Mirjana
AU  - Rat, Milica
AU  - Pezo, Lato
PY  - 2022
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5265
AB  - Unlike other studies that only determined the chemical composition of essential oils depending on their geographic origin, this research investigated the effect of weather conditions (temperature, precipitation, and insolation) on the chemical composition of Nepeta nuda L. essential oil. The collection of wild-growing N. nuda was carried out during three successive years, 2019, 2020, and 2021 at Rtanj Mountain (Serbia) on the same date (July 7th). Essential oil extraction from the plant was performed by hydro-distillation. After gas chromatographic-mass spectrometric analysis, a total of 102 volatile compounds were separated from N. nuda, during the observed period, 28 were unidentified, compromising between 5.0% and 8.7%, depending on the year. A multiple linear regression model was created, and statistical analyses were performed to provide knowledge about the prediction, feature profile, and the similarity in contents of active compounds of the N. nuda essential oil. The influence of temperature on the accumulation of the most abundant component, 1,8-cineole, was positive, while the impact of precipitation and insolation was negative. According to the cluster tree, there are four chemotypes of N. nuda essential oil: with nepetalactone, 1,8-cineole, mixed (nepetalactone+1,8-cineole+germacrene D), and nonspecific chemotypes. Bearing in mind that the biological activity of a raw material depends on the chemotype and environmental factors, this is a topic that deserves a more detailed approach. The N. nuda and its essential oil are promising materials with high biological potential, and these deserve further detailed investigation.
T2  - Horticulturae
T1  - Volatile Compounds of Nepeta nuda L. from Rtanj Mountain (Serbia)
VL  - 8
IS  - 2
SP  - 85
DO  - 10.3390/horticulturae8020085
ER  - 

@article{
author = "Aćimović, Milica and Lončar, Biljana and Pezo, Milada and Stanković Jeremić, Jovana and Cvetković, Mirjana and Rat, Milica and Pezo, Lato",
year = "2022",
abstract = "Unlike other studies that only determined the chemical composition of essential oils depending on their geographic origin, this research investigated the effect of weather conditions (temperature, precipitation, and insolation) on the chemical composition of Nepeta nuda L. essential oil. The collection of wild-growing N. nuda was carried out during three successive years, 2019, 2020, and 2021 at Rtanj Mountain (Serbia) on the same date (July 7th). Essential oil extraction from the plant was performed by hydro-distillation. After gas chromatographic-mass spectrometric analysis, a total of 102 volatile compounds were separated from N. nuda, during the observed period, 28 were unidentified, compromising between 5.0% and 8.7%, depending on the year. A multiple linear regression model was created, and statistical analyses were performed to provide knowledge about the prediction, feature profile, and the similarity in contents of active compounds of the N. nuda essential oil. The influence of temperature on the accumulation of the most abundant component, 1,8-cineole, was positive, while the impact of precipitation and insolation was negative. According to the cluster tree, there are four chemotypes of N. nuda essential oil: with nepetalactone, 1,8-cineole, mixed (nepetalactone+1,8-cineole+germacrene D), and nonspecific chemotypes. Bearing in mind that the biological activity of a raw material depends on the chemotype and environmental factors, this is a topic that deserves a more detailed approach. The N. nuda and its essential oil are promising materials with high biological potential, and these deserve further detailed investigation.",
journal = "Horticulturae",
title = "Volatile Compounds of Nepeta nuda L. from Rtanj Mountain (Serbia)",
volume = "8",
number = "2",
pages = "85",
doi = "10.3390/horticulturae8020085"
}

Aćimović, M., Lončar, B., Pezo, M., Stanković Jeremić, J., Cvetković, M., Rat, M.,& Pezo, L.. (2022). Volatile Compounds of Nepeta nuda L. from Rtanj Mountain (Serbia). in Horticulturae, 8(2), 85.
https://doi.org/10.3390/horticulturae8020085

Aćimović M, Lončar B, Pezo M, Stanković Jeremić J, Cvetković M, Rat M, Pezo L. Volatile Compounds of Nepeta nuda L. from Rtanj Mountain (Serbia). in Horticulturae. 2022;8(2):85.
doi:10.3390/horticulturae8020085 .

Aćimović, Milica, Lončar, Biljana, Pezo, Milada, Stanković Jeremić, Jovana, Cvetković, Mirjana, Rat, Milica, Pezo, Lato, "Volatile Compounds of Nepeta nuda L. from Rtanj Mountain (Serbia)" in Horticulturae, 8, no. 2 (2022):85,
https://doi.org/10.3390/horticulturae8020085 . .

TY  - JOUR
AU  - Aćimović, Milica
AU  - Pezo, Lato
AU  - Stanković Jeremić, Jovana
AU  - Todosijević, Marina
AU  - Rat, Milica
AU  - Tešević, Vele
AU  - Cvetković, Mirjana
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/5291
AB  - In the essential oil of yarrow (Achillea millefolium L. sensu lato) collected from natural popula-
tion on Mt. Rtanj (Serbia) and distilled by Clevenger apparatus 104 compounds were detected, and

the most abundant were camphor (9.8%), caryophyllene oxide (6.5%), terpinen-4-ol (6.3%) and 1,8-

cineole (5.6%). The quantitative structure-retention relationship (QSRR) model was employed to pre-
dict the retention indices, using four molecular descriptors selected by factor analysis and a genetic

algorithm. The coefficients of determination reached the value of 0.862, demonstrating that this mo-
del could be used for prediction purposes.
PB  - Novi Sad : Faculty of Technology
T2  - Acta Periodica Technologica
T1  - The quantitative structure retention relationship of the gs ms profile of yarrow essential oil
IS  - 52
SP  - 123
EP  - 132
DO  - 10.2298/APT2152123A
ER  - 

@article{
author = "Aćimović, Milica and Pezo, Lato and Stanković Jeremić, Jovana and Todosijević, Marina and Rat, Milica and Tešević, Vele and Cvetković, Mirjana",
year = "2021",
abstract = "In the essential oil of yarrow (Achillea millefolium L. sensu lato) collected from natural popula-
tion on Mt. Rtanj (Serbia) and distilled by Clevenger apparatus 104 compounds were detected, and

the most abundant were camphor (9.8%), caryophyllene oxide (6.5%), terpinen-4-ol (6.3%) and 1,8-

cineole (5.6%). The quantitative structure-retention relationship (QSRR) model was employed to pre-
dict the retention indices, using four molecular descriptors selected by factor analysis and a genetic

algorithm. The coefficients of determination reached the value of 0.862, demonstrating that this mo-
del could be used for prediction purposes.",
publisher = "Novi Sad : Faculty of Technology",
journal = "Acta Periodica Technologica",
title = "The quantitative structure retention relationship of the gs ms profile of yarrow essential oil",
number = "52",
pages = "123-132",
doi = "10.2298/APT2152123A"
}

Aćimović, M., Pezo, L., Stanković Jeremić, J., Todosijević, M., Rat, M., Tešević, V.,& Cvetković, M.. (2021). The quantitative structure retention relationship of the gs ms profile of yarrow essential oil. in Acta Periodica Technologica
Novi Sad : Faculty of Technology.(52), 123-132.
https://doi.org/10.2298/APT2152123A

Aćimović M, Pezo L, Stanković Jeremić J, Todosijević M, Rat M, Tešević V, Cvetković M. The quantitative structure retention relationship of the gs ms profile of yarrow essential oil. in Acta Periodica Technologica. 2021;(52):123-132.
doi:10.2298/APT2152123A .

Aćimović, Milica, Pezo, Lato, Stanković Jeremić, Jovana, Todosijević, Marina, Rat, Milica, Tešević, Vele, Cvetković, Mirjana, "The quantitative structure retention relationship of the gs ms profile of yarrow essential oil" in Acta Periodica Technologica, no. 52 (2021):123-132,
https://doi.org/10.2298/APT2152123A . .

TY  - JOUR
AU  - Aćimović, Milica
AU  - Pezo, Lato
AU  - Stanković, Jovana
AU  - Cvetković, Mirjana
AU  - Rat, Milica
AU  - Čabarkapa, Ivana
AU  - Tešević, Vele
PY  - 2020
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/3951
AB  - A total of 26 experimentally obtained retention indices on a logarithmic scale (log RI) from
Thymus serpyllum essential oil were used to build a robust predictive model. The selected descriptors were
used as inputs of an artificial neural network model to build a predictive quantitative structure-retention time
relationship model. The coefficient of determination for the training cycle was 0.977, indicating that this model
could be used for the prediction of retention indices for T. serpyllum essential oil compounds. These 26
compounds comprise about 99.8 % of the total oil, but among them only 6 compounds had the average relative
concentration over 1 percent: geraniol (63.4 %), nerol (or cis-geraniol) (18.9 %), geranyl acetate (4.7 %), transcaryophyllene (4.6 %), β-bisabolene (2.0 %) and geranial (1.2 %). According to these results, it can be concluded
that T. serpyllum from village Sesalac (Serbia) belongs to geraniol chemotype, in total 82.3 % in both, trans and
cis forms (63.4 % and 18.9 %, respectively).
PB  - Taylor & Francis
T2  - Essential Oil Bearing Plants
T1  - QSRR model for predicting retention indices of geraniol chemotype of Thymus serpyllum essential oil
VL  - 23
IS  - 3
SP  - 464
EP  - 473
DO  - 10.1080/0972060X.2020.1790428
ER  - 

@article{
author = "Aćimović, Milica and Pezo, Lato and Stanković, Jovana and Cvetković, Mirjana and Rat, Milica and Čabarkapa, Ivana and Tešević, Vele",
year = "2020",
abstract = "A total of 26 experimentally obtained retention indices on a logarithmic scale (log RI) from
Thymus serpyllum essential oil were used to build a robust predictive model. The selected descriptors were
used as inputs of an artificial neural network model to build a predictive quantitative structure-retention time
relationship model. The coefficient of determination for the training cycle was 0.977, indicating that this model
could be used for the prediction of retention indices for T. serpyllum essential oil compounds. These 26
compounds comprise about 99.8 % of the total oil, but among them only 6 compounds had the average relative
concentration over 1 percent: geraniol (63.4 %), nerol (or cis-geraniol) (18.9 %), geranyl acetate (4.7 %), transcaryophyllene (4.6 %), β-bisabolene (2.0 %) and geranial (1.2 %). According to these results, it can be concluded
that T. serpyllum from village Sesalac (Serbia) belongs to geraniol chemotype, in total 82.3 % in both, trans and
cis forms (63.4 % and 18.9 %, respectively).",
publisher = "Taylor & Francis",
journal = "Essential Oil Bearing Plants",
title = "QSRR model for predicting retention indices of geraniol chemotype of Thymus serpyllum essential oil",
volume = "23",
number = "3",
pages = "464-473",
doi = "10.1080/0972060X.2020.1790428"
}

Aćimović, M., Pezo, L., Stanković, J., Cvetković, M., Rat, M., Čabarkapa, I.,& Tešević, V.. (2020). QSRR model for predicting retention indices of geraniol chemotype of Thymus serpyllum essential oil. in Essential Oil Bearing Plants
Taylor & Francis., 23(3), 464-473.
https://doi.org/10.1080/0972060X.2020.1790428

Aćimović M, Pezo L, Stanković J, Cvetković M, Rat M, Čabarkapa I, Tešević V. QSRR model for predicting retention indices of geraniol chemotype of Thymus serpyllum essential oil. in Essential Oil Bearing Plants. 2020;23(3):464-473.
doi:10.1080/0972060X.2020.1790428 .

Aćimović, Milica, Pezo, Lato, Stanković, Jovana, Cvetković, Mirjana, Rat, Milica, Čabarkapa, Ivana, Tešević, Vele, "QSRR model for predicting retention indices of geraniol chemotype of Thymus serpyllum essential oil" in Essential Oil Bearing Plants, 23, no. 3 (2020):464-473,
https://doi.org/10.1080/0972060X.2020.1790428 . .