TY  - JOUR
AU  - Kozarski, Maja S.
AU  - Klaus, Anita
AU  - Vunduk, Jovana
AU  - Žižak, Željko
AU  - Nikšić, Miomir
AU  - Jakovljević, Dragica
AU  - Vrvić, Miroslav
AU  - Van Griensven, Leo J. L. D.
PY  - 2015
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1666
AB  - The methanolic extract of the wild edible mushroom Cantharellus cibarius Fr. (chanterelle) was analyzed for in vitro antioxidative, cytotoxic, antihypertensive and antibacterial activities. Various primary and secondary metabolites were found. Phenols were the major antioxidant components found in the extract (49.8 mg g(-1)), followed by flavonoids, whose content was approximately 86% of the total phenol content. Antioxidant activity, measured by four different methods, was high for inhibition of lipid peroxidation (EC50 = 1.21 mg mL(-1)) and chelating ability (EC50 = 0.64 mg mL(-1)). The antioxidant activity of the C. cibarius methanol extract was achieved through chelating iron compared to hydrogen atom and/or electron transfer. The extract showed good selectivity in cytotoxicity on human cervix adenocarcinoma HeLa, breast carcinoma MDA-MB-453 and human myelogenous leukemia K562, compared to normal control human fetal lung fibroblasts MRC-5 and human lung bronchial epithelial cells BEAS-2B. The extract had inhibitory activity against angiotensin converting I enzyme (ACE) (IC50 = 0.063 mg mL(-1)). The extract revealed selective antimicrobial activity against Gram-positive bacteria with the highest potential against E. faecalis. The medicinal and health benefits, observed in wild C. cibarius mushroom, seem an additional reason for its traditional use as a popular delicacy food.
PB  - Royal Soc Chemistry, Cambridge
T2  - Food & Function
T1  - Nutraceutical properties of the methanolic extract of edible mushroom Cantharellus cibarius (Fries): primary mechanisms
VL  - 6
IS  - 6
SP  - 1875
EP  - 1886
DO  - 10.1039/c5fo00312a
ER  - 

@article{
author = "Kozarski, Maja S. and Klaus, Anita and Vunduk, Jovana and Žižak, Željko and Nikšić, Miomir and Jakovljević, Dragica and Vrvić, Miroslav and Van Griensven, Leo J. L. D.",
year = "2015",
abstract = "The methanolic extract of the wild edible mushroom Cantharellus cibarius Fr. (chanterelle) was analyzed for in vitro antioxidative, cytotoxic, antihypertensive and antibacterial activities. Various primary and secondary metabolites were found. Phenols were the major antioxidant components found in the extract (49.8 mg g(-1)), followed by flavonoids, whose content was approximately 86% of the total phenol content. Antioxidant activity, measured by four different methods, was high for inhibition of lipid peroxidation (EC50 = 1.21 mg mL(-1)) and chelating ability (EC50 = 0.64 mg mL(-1)). The antioxidant activity of the C. cibarius methanol extract was achieved through chelating iron compared to hydrogen atom and/or electron transfer. The extract showed good selectivity in cytotoxicity on human cervix adenocarcinoma HeLa, breast carcinoma MDA-MB-453 and human myelogenous leukemia K562, compared to normal control human fetal lung fibroblasts MRC-5 and human lung bronchial epithelial cells BEAS-2B. The extract had inhibitory activity against angiotensin converting I enzyme (ACE) (IC50 = 0.063 mg mL(-1)). The extract revealed selective antimicrobial activity against Gram-positive bacteria with the highest potential against E. faecalis. The medicinal and health benefits, observed in wild C. cibarius mushroom, seem an additional reason for its traditional use as a popular delicacy food.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Food & Function",
title = "Nutraceutical properties of the methanolic extract of edible mushroom Cantharellus cibarius (Fries): primary mechanisms",
volume = "6",
number = "6",
pages = "1875-1886",
doi = "10.1039/c5fo00312a"
}

Kozarski, M. S., Klaus, A., Vunduk, J., Žižak, Ž., Nikšić, M., Jakovljević, D., Vrvić, M.,& Van Griensven, L. J. L. D.. (2015). Nutraceutical properties of the methanolic extract of edible mushroom Cantharellus cibarius (Fries): primary mechanisms. in Food & Function
Royal Soc Chemistry, Cambridge., 6(6), 1875-1886.
https://doi.org/10.1039/c5fo00312a

Kozarski MS, Klaus A, Vunduk J, Žižak Ž, Nikšić M, Jakovljević D, Vrvić M, Van Griensven LJLD. Nutraceutical properties of the methanolic extract of edible mushroom Cantharellus cibarius (Fries): primary mechanisms. in Food & Function. 2015;6(6):1875-1886.
doi:10.1039/c5fo00312a .

Kozarski, Maja S., Klaus, Anita, Vunduk, Jovana, Žižak, Željko, Nikšić, Miomir, Jakovljević, Dragica, Vrvić, Miroslav, Van Griensven, Leo J. L. D., "Nutraceutical properties of the methanolic extract of edible mushroom Cantharellus cibarius (Fries): primary mechanisms" in Food & Function, 6, no. 6 (2015):1875-1886,
https://doi.org/10.1039/c5fo00312a . .

TY  - JOUR
AU  - Klaus, Anita
AU  - Kozarski, Maja S.
AU  - Vunduk, Jovana
AU  - Todorović, Nina
AU  - Jakovljević, Dragica
AU  - Žižak, Željko
AU  - Pavlović, Vladimir B.
AU  - Levic, Steva
AU  - Nikšić, Miomir
AU  - Van Griensven, Leo J. L. D.
PY  - 2015
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1761
AB  - Partially purified polysaccharides (FP) and hot alkali extract (FNa) obtained from fruiting bodies of the wild basidiomycete Grifola frondosa were examined for their antimicrobial, antioxidant and cytotoxic activity. The structural properties of FP and FNa samples were investigated by FT-IR and high resolution 1H- and 13C-NMR spectroscopy. From a group of various G- and G+ bacteria the antibacterial effects were highest against the G + B. cereus. FNa was the better antioxidant shown by the lower EC50 values of DPPH scavenging ability, ferric-reducing antioxidant power and ferrous ion-chelating ability. Ferric-reducing antioxidant power and ferrous ion-chelating ability were mostly linked to total polysaccharides, total- and beta-glucan content, as well as total protein content. Both extracts displayed a moderate dose dependent antiproliferative action towards malignant human breast cancer MDA-MB-453, cervical adenocarcinoma HeLa and myelogenous leukemia K562 cells not observed in the non cancer derived MRC-5 fibroblasts. The highest effect was found in HeLa cells for FP extract. The mean diameter of Ca-alginate bead loading FP was 960.7 mu m while the mean diameter of beads encapsulating FNa extract was 1051.7 mu m.
PB  - Elsevier
T2  - Food Research International
T1  - Biological potential of extracts of the wild edible Basidiomycete mushroom Grifola frondosa
VL  - 67
SP  - 272
EP  - 283
DO  - 10.1016/j.foodres.2014.11.035
ER  - 

@article{
author = "Klaus, Anita and Kozarski, Maja S. and Vunduk, Jovana and Todorović, Nina and Jakovljević, Dragica and Žižak, Željko and Pavlović, Vladimir B. and Levic, Steva and Nikšić, Miomir and Van Griensven, Leo J. L. D.",
year = "2015",
abstract = "Partially purified polysaccharides (FP) and hot alkali extract (FNa) obtained from fruiting bodies of the wild basidiomycete Grifola frondosa were examined for their antimicrobial, antioxidant and cytotoxic activity. The structural properties of FP and FNa samples were investigated by FT-IR and high resolution 1H- and 13C-NMR spectroscopy. From a group of various G- and G+ bacteria the antibacterial effects were highest against the G + B. cereus. FNa was the better antioxidant shown by the lower EC50 values of DPPH scavenging ability, ferric-reducing antioxidant power and ferrous ion-chelating ability. Ferric-reducing antioxidant power and ferrous ion-chelating ability were mostly linked to total polysaccharides, total- and beta-glucan content, as well as total protein content. Both extracts displayed a moderate dose dependent antiproliferative action towards malignant human breast cancer MDA-MB-453, cervical adenocarcinoma HeLa and myelogenous leukemia K562 cells not observed in the non cancer derived MRC-5 fibroblasts. The highest effect was found in HeLa cells for FP extract. The mean diameter of Ca-alginate bead loading FP was 960.7 mu m while the mean diameter of beads encapsulating FNa extract was 1051.7 mu m.",
publisher = "Elsevier",
journal = "Food Research International",
title = "Biological potential of extracts of the wild edible Basidiomycete mushroom Grifola frondosa",
volume = "67",
pages = "272-283",
doi = "10.1016/j.foodres.2014.11.035"
}

Klaus, A., Kozarski, M. S., Vunduk, J., Todorović, N., Jakovljević, D., Žižak, Ž., Pavlović, V. B., Levic, S., Nikšić, M.,& Van Griensven, L. J. L. D.. (2015). Biological potential of extracts of the wild edible Basidiomycete mushroom Grifola frondosa. in Food Research International
Elsevier., 67, 272-283.
https://doi.org/10.1016/j.foodres.2014.11.035

Klaus A, Kozarski MS, Vunduk J, Todorović N, Jakovljević D, Žižak Ž, Pavlović VB, Levic S, Nikšić M, Van Griensven LJLD. Biological potential of extracts of the wild edible Basidiomycete mushroom Grifola frondosa. in Food Research International. 2015;67:272-283.
doi:10.1016/j.foodres.2014.11.035 .

Klaus, Anita, Kozarski, Maja S., Vunduk, Jovana, Todorović, Nina, Jakovljević, Dragica, Žižak, Željko, Pavlović, Vladimir B., Levic, Steva, Nikšić, Miomir, Van Griensven, Leo J. L. D., "Biological potential of extracts of the wild edible Basidiomycete mushroom Grifola frondosa" in Food Research International, 67 (2015):272-283,
https://doi.org/10.1016/j.foodres.2014.11.035 . .

TY  - JOUR
AU  - Kozarski, Maja S.
AU  - Klaus, Anita
AU  - Jakovljević, Dragica
AU  - Todorović, Nina
AU  - Vunduk, Jovana
AU  - Petrović, Predrag
AU  - Nikšić, Miomir
AU  - Vrvić, Miroslav
AU  - Van Griensven, Leo J. L. D.
PY  - 2015
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1826
AB  - Oxidative stress caused by an imbalanced metabolism and an excess of reactive oxygen species (ROS) lead to a range of health disorders in humans. Our endogenous antioxidant defense mechanisms and our dietary intake of antioxidants potentially regulate our oxidative homeostasis. Numerous synthetic antioxidants can effectively improve defense mechanisms, but because of their adverse toxic effects under certain conditions, preference is given to natural compounds. Consequently, the requirements for natural, alternative sources of antioxidant foods identified in edible mushrooms, as well as the mechanistic action involved in their antioxidant properties, have increased rapidly. Chemical composition and antioxidant potential of mushrooms have been intensively studied. Edible mushrooms might be used directly in enhancement of antioxidant defenses through dietary supplementation to reduce the level of oxidative stress. Wild or cultivated, they have been related to significant antioxidant properties due to their bioactive compounds, such as polyphenols, polysaccharides, vitamins, carotenoids and minerals. Antioxidant and health benefits, observed in edible mushrooms, seem an additional reason for their traditional use as a popular delicacy food. This review discusses the consumption of edible mushrooms as a powerful instrument in maintaining health, longevity and life quality.
PB  - MDPI
T2  - Molecules
T1  - Antioxidants of Edible Mushrooms
VL  - 20
IS  - 10
SP  - 19489
EP  - 19525
DO  - 10.3390/molecules201019489
ER  - 

@article{
author = "Kozarski, Maja S. and Klaus, Anita and Jakovljević, Dragica and Todorović, Nina and Vunduk, Jovana and Petrović, Predrag and Nikšić, Miomir and Vrvić, Miroslav and Van Griensven, Leo J. L. D.",
year = "2015",
abstract = "Oxidative stress caused by an imbalanced metabolism and an excess of reactive oxygen species (ROS) lead to a range of health disorders in humans. Our endogenous antioxidant defense mechanisms and our dietary intake of antioxidants potentially regulate our oxidative homeostasis. Numerous synthetic antioxidants can effectively improve defense mechanisms, but because of their adverse toxic effects under certain conditions, preference is given to natural compounds. Consequently, the requirements for natural, alternative sources of antioxidant foods identified in edible mushrooms, as well as the mechanistic action involved in their antioxidant properties, have increased rapidly. Chemical composition and antioxidant potential of mushrooms have been intensively studied. Edible mushrooms might be used directly in enhancement of antioxidant defenses through dietary supplementation to reduce the level of oxidative stress. Wild or cultivated, they have been related to significant antioxidant properties due to their bioactive compounds, such as polyphenols, polysaccharides, vitamins, carotenoids and minerals. Antioxidant and health benefits, observed in edible mushrooms, seem an additional reason for their traditional use as a popular delicacy food. This review discusses the consumption of edible mushrooms as a powerful instrument in maintaining health, longevity and life quality.",
publisher = "MDPI",
journal = "Molecules",
title = "Antioxidants of Edible Mushrooms",
volume = "20",
number = "10",
pages = "19489-19525",
doi = "10.3390/molecules201019489"
}

Kozarski, M. S., Klaus, A., Jakovljević, D., Todorović, N., Vunduk, J., Petrović, P., Nikšić, M., Vrvić, M.,& Van Griensven, L. J. L. D.. (2015). Antioxidants of Edible Mushrooms. in Molecules
MDPI., 20(10), 19489-19525.
https://doi.org/10.3390/molecules201019489

Kozarski MS, Klaus A, Jakovljević D, Todorović N, Vunduk J, Petrović P, Nikšić M, Vrvić M, Van Griensven LJLD. Antioxidants of Edible Mushrooms. in Molecules. 2015;20(10):19489-19525.
doi:10.3390/molecules201019489 .

Kozarski, Maja S., Klaus, Anita, Jakovljević, Dragica, Todorović, Nina, Vunduk, Jovana, Petrović, Predrag, Nikšić, Miomir, Vrvić, Miroslav, Van Griensven, Leo J. L. D., "Antioxidants of Edible Mushrooms" in Molecules, 20, no. 10 (2015):19489-19525,
https://doi.org/10.3390/molecules201019489 . .

TY  - JOUR
AU  - Kozarski, Maja S.
AU  - Klaus, Anita
AU  - Jakovljević, Dragica
AU  - Todorović, Nina
AU  - Nikšić, Miomir
AU  - Vrvić, Miroslav
AU  - Van Griensven, Leo J. L. D.
PY  - 2014
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1395
AB  - Polysaccharides of the European strain of A. brasiliensis were obtained by hot water extraction and ethanol precipitation (HWPE I) of fruiting bodies, and further purified by dialysis (HWPE II) and pronase incubation (PPE). These polysaccharides consisted mainly of (1 -> 6)-beta-D-glucans. PPE was free of proteins and polyphenols as demonstrated by quantitative assays and NMR profiling. They showed a clear IFN-gamma inducing activity in human PBMCs, which suggests these polysaccharides to have proinflammatory effects. Treatment by beta-glucosidase caused the polysaccharides to be degraded into smaller fragments and at the same time increased their IFN-gamma inducing activity in PBMCs fourfold. In vitro, PPE showed a dose-dependent inhibition of the proliferation of the human leukemia Jurkat cell. At 100 mu g/mL the cells' viability was decreased by appr. 51% compared to the control. EPR spin trapping demonstrated a high antioxidative activity against (OH)-O-center dot and (center dot)O2(-) radicals of HWPE land PPE. Further, the results of the antioxidant assays indicated that antioxidant activity against (OH)-O-center dot radicals in the Fenton system was achieved through scavenging or through chelating iron mechanisms. The good immunomodulating and antioxidative properties of A. brasiliensis polysaccharide extract obtained by hot water extraction and ethanol precipitation make it suitable for everyday use as an inexpensive dietary supplement.
PB  - Elsevier
T2  - Food Research International
T1  - Dietary polysaccharide extracts of Agaricus brasiliensis fruiting bodies: chemical characterization and bioactivities at different levels of purification
VL  - 64
SP  - 53
EP  - 64
DO  - 10.1016/j.foodres.2014.05.075
ER  - 

@article{
author = "Kozarski, Maja S. and Klaus, Anita and Jakovljević, Dragica and Todorović, Nina and Nikšić, Miomir and Vrvić, Miroslav and Van Griensven, Leo J. L. D.",
year = "2014",
abstract = "Polysaccharides of the European strain of A. brasiliensis were obtained by hot water extraction and ethanol precipitation (HWPE I) of fruiting bodies, and further purified by dialysis (HWPE II) and pronase incubation (PPE). These polysaccharides consisted mainly of (1 -> 6)-beta-D-glucans. PPE was free of proteins and polyphenols as demonstrated by quantitative assays and NMR profiling. They showed a clear IFN-gamma inducing activity in human PBMCs, which suggests these polysaccharides to have proinflammatory effects. Treatment by beta-glucosidase caused the polysaccharides to be degraded into smaller fragments and at the same time increased their IFN-gamma inducing activity in PBMCs fourfold. In vitro, PPE showed a dose-dependent inhibition of the proliferation of the human leukemia Jurkat cell. At 100 mu g/mL the cells' viability was decreased by appr. 51% compared to the control. EPR spin trapping demonstrated a high antioxidative activity against (OH)-O-center dot and (center dot)O2(-) radicals of HWPE land PPE. Further, the results of the antioxidant assays indicated that antioxidant activity against (OH)-O-center dot radicals in the Fenton system was achieved through scavenging or through chelating iron mechanisms. The good immunomodulating and antioxidative properties of A. brasiliensis polysaccharide extract obtained by hot water extraction and ethanol precipitation make it suitable for everyday use as an inexpensive dietary supplement.",
publisher = "Elsevier",
journal = "Food Research International",
title = "Dietary polysaccharide extracts of Agaricus brasiliensis fruiting bodies: chemical characterization and bioactivities at different levels of purification",
volume = "64",
pages = "53-64",
doi = "10.1016/j.foodres.2014.05.075"
}

Kozarski, M. S., Klaus, A., Jakovljević, D., Todorović, N., Nikšić, M., Vrvić, M.,& Van Griensven, L. J. L. D.. (2014). Dietary polysaccharide extracts of Agaricus brasiliensis fruiting bodies: chemical characterization and bioactivities at different levels of purification. in Food Research International
Elsevier., 64, 53-64.
https://doi.org/10.1016/j.foodres.2014.05.075

Kozarski MS, Klaus A, Jakovljević D, Todorović N, Nikšić M, Vrvić M, Van Griensven LJLD. Dietary polysaccharide extracts of Agaricus brasiliensis fruiting bodies: chemical characterization and bioactivities at different levels of purification. in Food Research International. 2014;64:53-64.
doi:10.1016/j.foodres.2014.05.075 .

Kozarski, Maja S., Klaus, Anita, Jakovljević, Dragica, Todorović, Nina, Nikšić, Miomir, Vrvić, Miroslav, Van Griensven, Leo J. L. D., "Dietary polysaccharide extracts of Agaricus brasiliensis fruiting bodies: chemical characterization and bioactivities at different levels of purification" in Food Research International, 64 (2014):53-64,
https://doi.org/10.1016/j.foodres.2014.05.075 . .

TY  - JOUR
AU  - Kozarski, Maja S.
AU  - Klaus, Anita
AU  - Nikšić, Miomir
AU  - Van Griensven, Leo J. L. D.
AU  - Vrvić, Miroslav
AU  - Jakovljević, Dragica
PY  - 2014
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1587
AB  - The fungal polysaccharides attract a lot of attention due to their multiple challenging biological properties, such as: anti-tumor, anti-viral, anticomplementary, anticoagulant, hypolipidemic, immunomodulatory and immune-stimulatory activities, which all together make them suitable for application in many quite distinctive areas, such as food industry, biomedicine, cosmetology, agriculture, environmental protection and waste water management. This article presents results with respect to biological properties, structure and procedures related to the isolation and activation of polysaccharides of higher fungi. It is considered and presented along with a review of the critical antioxidative activity and possible influence of the structural composition of polysaccharide extracts (isolated from these higher fungi) upon their antioxidative properties.
PB  - Association of Chemical Engineers of Serbia
T2  - Hemijska industrija
T1  - Polysaccharides of higher fungi: biological role, structure and antioxidative activity
VL  - 68
IS  - 3
SP  - 305
EP  - 320
DO  - 10.2298/HEMIND121114056K
ER  - 

@article{
author = "Kozarski, Maja S. and Klaus, Anita and Nikšić, Miomir and Van Griensven, Leo J. L. D. and Vrvić, Miroslav and Jakovljević, Dragica",
year = "2014",
abstract = "The fungal polysaccharides attract a lot of attention due to their multiple challenging biological properties, such as: anti-tumor, anti-viral, anticomplementary, anticoagulant, hypolipidemic, immunomodulatory and immune-stimulatory activities, which all together make them suitable for application in many quite distinctive areas, such as food industry, biomedicine, cosmetology, agriculture, environmental protection and waste water management. This article presents results with respect to biological properties, structure and procedures related to the isolation and activation of polysaccharides of higher fungi. It is considered and presented along with a review of the critical antioxidative activity and possible influence of the structural composition of polysaccharide extracts (isolated from these higher fungi) upon their antioxidative properties.",
publisher = "Association of Chemical Engineers of Serbia",
journal = "Hemijska industrija",
title = "Polysaccharides of higher fungi: biological role, structure and antioxidative activity",
volume = "68",
number = "3",
pages = "305-320",
doi = "10.2298/HEMIND121114056K"
}

Kozarski, M. S., Klaus, A., Nikšić, M., Van Griensven, L. J. L. D., Vrvić, M.,& Jakovljević, D.. (2014). Polysaccharides of higher fungi: biological role, structure and antioxidative activity. in Hemijska industrija
Association of Chemical Engineers of Serbia., 68(3), 305-320.
https://doi.org/10.2298/HEMIND121114056K

Kozarski MS, Klaus A, Nikšić M, Van Griensven LJLD, Vrvić M, Jakovljević D. Polysaccharides of higher fungi: biological role, structure and antioxidative activity. in Hemijska industrija. 2014;68(3):305-320.
doi:10.2298/HEMIND121114056K .

Kozarski, Maja S., Klaus, Anita, Nikšić, Miomir, Van Griensven, Leo J. L. D., Vrvić, Miroslav, Jakovljević, Dragica, "Polysaccharides of higher fungi: biological role, structure and antioxidative activity" in Hemijska industrija, 68, no. 3 (2014):305-320,
https://doi.org/10.2298/HEMIND121114056K . .

TY  - JOUR
AU  - Klaus, Anita
AU  - Kozarski, Maja S.
AU  - Nikšić, Miomir
AU  - Jakovljević, Dragica
AU  - Todorović, Nina
AU  - Stefanoska, Ivana
AU  - Van Griensven, Leo J. L. D.
PY  - 2013
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/1196
AB  - Hot water extract (LN), partially purified polysaccharides (LP) and hot alkali extracted polysaccharides (LNa) obtained from fruiting bodies of the wild basidiomycete Laetiporus sulphureus were examined for their antioxidant activities. LNa was the most active antioxidant, as shown by the median effective concentrations (EC50 values) of 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity (0.5 +/- 0.2 mg/ml), reducing power (4.0 +/- 0.3 mg/ml) and ferrous ion-chelating ability (1.5 +/- 0.1 mg/ml). LNa contained the highest level of alpha-glucan (17.3 +/- 1.2 g/100 g dw), whereas LP contained mostly beta-glucans (66.8 +/- 1.3 g/100 g dw). The prevalent monosaccharide in all extracts was glucose. The EC50 values of all three antioxidant activity assays were well-correlated with the alpha-glucan content. Strong and significant correlation was found between total phenolic compounds and DPPH scavenging ability and also reducing power. The three investigated extracts (at concentrations of 0.1-10 mg/ml) were not toxic to HTR-8/SVneo trophoblast cell line.
PB  - Informa Healthcare, London
T2  - International Journal of Food Sciences and Nutrition
T1  - The edible mushroom Laetiporus sulphureus as potential source of natural antioxidants
VL  - 64
IS  - 5
SP  - 599
EP  - 610
DO  - 10.3109/09637486.2012.759190
ER  - 

@article{
author = "Klaus, Anita and Kozarski, Maja S. and Nikšić, Miomir and Jakovljević, Dragica and Todorović, Nina and Stefanoska, Ivana and Van Griensven, Leo J. L. D.",
year = "2013",
abstract = "Hot water extract (LN), partially purified polysaccharides (LP) and hot alkali extracted polysaccharides (LNa) obtained from fruiting bodies of the wild basidiomycete Laetiporus sulphureus were examined for their antioxidant activities. LNa was the most active antioxidant, as shown by the median effective concentrations (EC50 values) of 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity (0.5 +/- 0.2 mg/ml), reducing power (4.0 +/- 0.3 mg/ml) and ferrous ion-chelating ability (1.5 +/- 0.1 mg/ml). LNa contained the highest level of alpha-glucan (17.3 +/- 1.2 g/100 g dw), whereas LP contained mostly beta-glucans (66.8 +/- 1.3 g/100 g dw). The prevalent monosaccharide in all extracts was glucose. The EC50 values of all three antioxidant activity assays were well-correlated with the alpha-glucan content. Strong and significant correlation was found between total phenolic compounds and DPPH scavenging ability and also reducing power. The three investigated extracts (at concentrations of 0.1-10 mg/ml) were not toxic to HTR-8/SVneo trophoblast cell line.",
publisher = "Informa Healthcare, London",
journal = "International Journal of Food Sciences and Nutrition",
title = "The edible mushroom Laetiporus sulphureus as potential source of natural antioxidants",
volume = "64",
number = "5",
pages = "599-610",
doi = "10.3109/09637486.2012.759190"
}

Klaus, A., Kozarski, M. S., Nikšić, M., Jakovljević, D., Todorović, N., Stefanoska, I.,& Van Griensven, L. J. L. D.. (2013). The edible mushroom Laetiporus sulphureus as potential source of natural antioxidants. in International Journal of Food Sciences and Nutrition
Informa Healthcare, London., 64(5), 599-610.
https://doi.org/10.3109/09637486.2012.759190

Klaus A, Kozarski MS, Nikšić M, Jakovljević D, Todorović N, Stefanoska I, Van Griensven LJLD. The edible mushroom Laetiporus sulphureus as potential source of natural antioxidants. in International Journal of Food Sciences and Nutrition. 2013;64(5):599-610.
doi:10.3109/09637486.2012.759190 .

Klaus, Anita, Kozarski, Maja S., Nikšić, Miomir, Jakovljević, Dragica, Todorović, Nina, Stefanoska, Ivana, Van Griensven, Leo J. L. D., "The edible mushroom Laetiporus sulphureus as potential source of natural antioxidants" in International Journal of Food Sciences and Nutrition, 64, no. 5 (2013):599-610,
https://doi.org/10.3109/09637486.2012.759190 . .

TY  - JOUR
AU  - Kozarski, Maja S.
AU  - Klaus, Anita
AU  - Nikšić, Miomir
AU  - Vrvić, Miroslav
AU  - Todorović, Nina
AU  - Jakovljević, Dragica
AU  - Van Griensven, Leo J. L. D.
PY  - 2012
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/987
AB  - Antioxidant activities of polysaccharide extracts of four of the most widely known mushrooms often used in medicinal applications as well as in tea and food, namely Ganoderma applanatum, Ganoderma lucidum, Lentinus edodes and Trametes versicolor, were studied. G. applanatum and L edodes extracts were the strongest DPPH scavengers, i.e. EC50  LT  0.1 mg/mL. G. applanatum extract had the highest chelating ability of ferrous ion (EC50 = 3.58 mg/mL), inhibition of lipid peroxidation (EC50 = 2.07 mg/mL) and had the highest reducing power, EC50 = 0.18. All four polysaccharide extracts showed a strong correlation between the reducing power and the total amount of phenols and alpha-glucans: no such correlation was found between the reducing power and the amount of total polysaccharides and proteins. The extracts contained a mixture/complex of polysaccharides, proteins and polyphenols, still present after hot water treatment, ethanol precipitation and dialysis. Glucose was the prevalent monosaccharide of all polysaccharides and samples contained both alpha- and beta-glucans. Total glucan contents varied considerably, i.e. from 35% for G. applanatum to 83% for G. lucidum. Uronic acid was found in the G. applanatum polysaccharides. Fourier transform infrared (FT-IR) analysis showed strong aromatic and ester peaks next to the different sugar and polysaccharide signals.
PB  - Academic Press Inc.
T2  - Journal of Food Composition and Analysis
T1  - Antioxidative activities and chemical characterization of polysaccharide extracts from the widely used mushrooms Ganoderma applanatum, Ganoderma lucidum, Lentinus edodes and Trametes versicolor
VL  - 26
IS  - 1-2
SP  - 144
EP  - 153
DO  - 10.1016/j.jfca.2012.02.004
ER  - 

@article{
author = "Kozarski, Maja S. and Klaus, Anita and Nikšić, Miomir and Vrvić, Miroslav and Todorović, Nina and Jakovljević, Dragica and Van Griensven, Leo J. L. D.",
year = "2012",
abstract = "Antioxidant activities of polysaccharide extracts of four of the most widely known mushrooms often used in medicinal applications as well as in tea and food, namely Ganoderma applanatum, Ganoderma lucidum, Lentinus edodes and Trametes versicolor, were studied. G. applanatum and L edodes extracts were the strongest DPPH scavengers, i.e. EC50  LT  0.1 mg/mL. G. applanatum extract had the highest chelating ability of ferrous ion (EC50 = 3.58 mg/mL), inhibition of lipid peroxidation (EC50 = 2.07 mg/mL) and had the highest reducing power, EC50 = 0.18. All four polysaccharide extracts showed a strong correlation between the reducing power and the total amount of phenols and alpha-glucans: no such correlation was found between the reducing power and the amount of total polysaccharides and proteins. The extracts contained a mixture/complex of polysaccharides, proteins and polyphenols, still present after hot water treatment, ethanol precipitation and dialysis. Glucose was the prevalent monosaccharide of all polysaccharides and samples contained both alpha- and beta-glucans. Total glucan contents varied considerably, i.e. from 35% for G. applanatum to 83% for G. lucidum. Uronic acid was found in the G. applanatum polysaccharides. Fourier transform infrared (FT-IR) analysis showed strong aromatic and ester peaks next to the different sugar and polysaccharide signals.",
publisher = "Academic Press Inc.",
journal = "Journal of Food Composition and Analysis",
title = "Antioxidative activities and chemical characterization of polysaccharide extracts from the widely used mushrooms Ganoderma applanatum, Ganoderma lucidum, Lentinus edodes and Trametes versicolor",
volume = "26",
number = "1-2",
pages = "144-153",
doi = "10.1016/j.jfca.2012.02.004"
}

Kozarski, M. S., Klaus, A., Nikšić, M., Vrvić, M., Todorović, N., Jakovljević, D.,& Van Griensven, L. J. L. D.. (2012). Antioxidative activities and chemical characterization of polysaccharide extracts from the widely used mushrooms Ganoderma applanatum, Ganoderma lucidum, Lentinus edodes and Trametes versicolor. in Journal of Food Composition and Analysis
Academic Press Inc.., 26(1-2), 144-153.
https://doi.org/10.1016/j.jfca.2012.02.004

Kozarski MS, Klaus A, Nikšić M, Vrvić M, Todorović N, Jakovljević D, Van Griensven LJLD. Antioxidative activities and chemical characterization of polysaccharide extracts from the widely used mushrooms Ganoderma applanatum, Ganoderma lucidum, Lentinus edodes and Trametes versicolor. in Journal of Food Composition and Analysis. 2012;26(1-2):144-153.
doi:10.1016/j.jfca.2012.02.004 .

Kozarski, Maja S., Klaus, Anita, Nikšić, Miomir, Vrvić, Miroslav, Todorović, Nina, Jakovljević, Dragica, Van Griensven, Leo J. L. D., "Antioxidative activities and chemical characterization of polysaccharide extracts from the widely used mushrooms Ganoderma applanatum, Ganoderma lucidum, Lentinus edodes and Trametes versicolor" in Journal of Food Composition and Analysis, 26, no. 1-2 (2012):144-153,
https://doi.org/10.1016/j.jfca.2012.02.004 . .

TY  - JOUR
AU  - Klaus, Anita
AU  - Kozarski, Maja S.
AU  - Nikšić, Miomir
AU  - Jakovljević, Dragica
AU  - Todorović, Nina
AU  - Van Griensven, Leo J. L. D.
PY  - 2011
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/853
AB  - Antioxidant properties properties of hot water extract (HWE), hot water extracted polysaccharides (HWP) and hot alkali extracted polysaccharides (HWAE) were obtained from fruiting bodies of the wild basidiomycete Schizophyllum commune. All extracts contained both alpha- and beta-glucans as determined by Megazyme beta-glucan assay kit. Glucose was found by TLC and NMR to be the prevalent monosaccharide in all extracts. Total phenol contents were in descending order of HWP approximate to HWE > HWAE. Median effective concentrations (EC(50) values) of antioxidant activities were 8.3 +/- 0.1, 6.9 +/- 0.0 and 8.9 +/- 0.1 mg/mL, and of the DPPH scavenging activity 0.8 +/- 0.0, 0.6 +/- 0.0 and 1.8 +/- 0.0 mg/mL, for HWE, HWP and HWAE, respectively. EC(50) values of reducing power were 7.6 +/- 0.1, 7.9 +/- 0.0 and 12.5 +/- 0.1 mg/mL, whereas those of the chelating abilities on ferrous ions were 3.1 +/- 0.0, 4.6 +/- 0.1 and 4.9 +/- 0.1 mg/mL The EC(50) values of the antioxidant activity, of the DPPH scavenging, and of the reducing power were correlated with total polysaccharide as well as with total phenol content. The antioxidant activities of all the extracts may be caused by both polysaccharides and polyphenols or by a complex of both.
PB  - Elsevier
T2  - Lwt-Food Science and Technology
T1  - Antioxidative activities and chemical characterization of polysaccharides extracted from the basidiomycete Schizophyllum commune
VL  - 44
IS  - 10
SP  - 2005
EP  - 2011
DO  - 10.1016/j.lwt.2011.05.010
ER  - 

@article{
author = "Klaus, Anita and Kozarski, Maja S. and Nikšić, Miomir and Jakovljević, Dragica and Todorović, Nina and Van Griensven, Leo J. L. D.",
year = "2011",
abstract = "Antioxidant properties properties of hot water extract (HWE), hot water extracted polysaccharides (HWP) and hot alkali extracted polysaccharides (HWAE) were obtained from fruiting bodies of the wild basidiomycete Schizophyllum commune. All extracts contained both alpha- and beta-glucans as determined by Megazyme beta-glucan assay kit. Glucose was found by TLC and NMR to be the prevalent monosaccharide in all extracts. Total phenol contents were in descending order of HWP approximate to HWE > HWAE. Median effective concentrations (EC(50) values) of antioxidant activities were 8.3 +/- 0.1, 6.9 +/- 0.0 and 8.9 +/- 0.1 mg/mL, and of the DPPH scavenging activity 0.8 +/- 0.0, 0.6 +/- 0.0 and 1.8 +/- 0.0 mg/mL, for HWE, HWP and HWAE, respectively. EC(50) values of reducing power were 7.6 +/- 0.1, 7.9 +/- 0.0 and 12.5 +/- 0.1 mg/mL, whereas those of the chelating abilities on ferrous ions were 3.1 +/- 0.0, 4.6 +/- 0.1 and 4.9 +/- 0.1 mg/mL The EC(50) values of the antioxidant activity, of the DPPH scavenging, and of the reducing power were correlated with total polysaccharide as well as with total phenol content. The antioxidant activities of all the extracts may be caused by both polysaccharides and polyphenols or by a complex of both.",
publisher = "Elsevier",
journal = "Lwt-Food Science and Technology",
title = "Antioxidative activities and chemical characterization of polysaccharides extracted from the basidiomycete Schizophyllum commune",
volume = "44",
number = "10",
pages = "2005-2011",
doi = "10.1016/j.lwt.2011.05.010"
}

Klaus, A., Kozarski, M. S., Nikšić, M., Jakovljević, D., Todorović, N.,& Van Griensven, L. J. L. D.. (2011). Antioxidative activities and chemical characterization of polysaccharides extracted from the basidiomycete Schizophyllum commune. in Lwt-Food Science and Technology
Elsevier., 44(10), 2005-2011.
https://doi.org/10.1016/j.lwt.2011.05.010

Klaus A, Kozarski MS, Nikšić M, Jakovljević D, Todorović N, Van Griensven LJLD. Antioxidative activities and chemical characterization of polysaccharides extracted from the basidiomycete Schizophyllum commune. in Lwt-Food Science and Technology. 2011;44(10):2005-2011.
doi:10.1016/j.lwt.2011.05.010 .

Klaus, Anita, Kozarski, Maja S., Nikšić, Miomir, Jakovljević, Dragica, Todorović, Nina, Van Griensven, Leo J. L. D., "Antioxidative activities and chemical characterization of polysaccharides extracted from the basidiomycete Schizophyllum commune" in Lwt-Food Science and Technology, 44, no. 10 (2011):2005-2011,
https://doi.org/10.1016/j.lwt.2011.05.010 . .

TY  - JOUR
AU  - Kozarski, Maja S.
AU  - Klaus, Anita
AU  - Nikšić, Miomir
AU  - Jakovljević, Dragica
AU  - Helsper, Johannes P. F. G.
AU  - Van Griensven, Leo J. L. D.
PY  - 2011
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/856
AB  - Partially purified polysaccharides were obtained from four medicinal mushroom species, Agaricus bisporus, Agaricus brasiliensis, Phellinus linteus and Ganoderma lucidum by hot water extraction, followed by ethanol precipitation. The four samples contained varying amounts of both alpha- and beta-glucans as determined by FT-IR and by quantitative estimation after prior partial hydrolysis (Megazyme beta-glucan assay kit). EC50 values of the DPPH scavenging activity of the polysaccharides from G. lucidum spores and P. linteus fruiting bodies were found to be particularly low, i.e. EC50  LT  0.1 mg/ml. For A. brasiliensis and A. bisporus, EC50 values were 0.27 and 2.0 mg/ml. EC50 values of the antioxidant activity were 7.07 mg/ml for G. lucidum, 13.25 mg/ml for A. brasiliensis and >20 mg/ml for A. bisporus polysaccharide, respectively. EC50 values of the chelating activity of ferrous ions ranged from 0.59 mg/ml for G. lucidum to 7.80 mg/ml for A. bisporus. The EC50 values of the extracts in the reducing power assay ranged from 0.47 to 14.83 mg/ml. A correlation was found between EC50 values of the chelating and reducing power abilities and the amount of total glucans content in the extracts. In vitro measurements of immunomodulatory capacity of polysaccharide extracts showed that A. bisporus, A. brasiliensis fruiting bodies and G. lucidum spores extracts express an immunostimulating effect on activated human PBMCs and induce synthesis of IFN-gamma. The polysaccharide extract of P. linteus fruiting bodies showed an immunosuppressive effect.
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Chemistry
T1  - Antioxidative and immunomodulating activities of polysaccharide extracts of the medicinal mushrooms Agaricus bisporus, Agaricus brasiliensis, Ganoderma lucidum and Phellinus linteus
VL  - 129
IS  - 4
SP  - 1667
EP  - 1675
DO  - 10.1016/j.foodchem.2011.06.029
ER  - 

@article{
author = "Kozarski, Maja S. and Klaus, Anita and Nikšić, Miomir and Jakovljević, Dragica and Helsper, Johannes P. F. G. and Van Griensven, Leo J. L. D.",
year = "2011",
abstract = "Partially purified polysaccharides were obtained from four medicinal mushroom species, Agaricus bisporus, Agaricus brasiliensis, Phellinus linteus and Ganoderma lucidum by hot water extraction, followed by ethanol precipitation. The four samples contained varying amounts of both alpha- and beta-glucans as determined by FT-IR and by quantitative estimation after prior partial hydrolysis (Megazyme beta-glucan assay kit). EC50 values of the DPPH scavenging activity of the polysaccharides from G. lucidum spores and P. linteus fruiting bodies were found to be particularly low, i.e. EC50  LT  0.1 mg/ml. For A. brasiliensis and A. bisporus, EC50 values were 0.27 and 2.0 mg/ml. EC50 values of the antioxidant activity were 7.07 mg/ml for G. lucidum, 13.25 mg/ml for A. brasiliensis and >20 mg/ml for A. bisporus polysaccharide, respectively. EC50 values of the chelating activity of ferrous ions ranged from 0.59 mg/ml for G. lucidum to 7.80 mg/ml for A. bisporus. The EC50 values of the extracts in the reducing power assay ranged from 0.47 to 14.83 mg/ml. A correlation was found between EC50 values of the chelating and reducing power abilities and the amount of total glucans content in the extracts. In vitro measurements of immunomodulatory capacity of polysaccharide extracts showed that A. bisporus, A. brasiliensis fruiting bodies and G. lucidum spores extracts express an immunostimulating effect on activated human PBMCs and induce synthesis of IFN-gamma. The polysaccharide extract of P. linteus fruiting bodies showed an immunosuppressive effect.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Chemistry",
title = "Antioxidative and immunomodulating activities of polysaccharide extracts of the medicinal mushrooms Agaricus bisporus, Agaricus brasiliensis, Ganoderma lucidum and Phellinus linteus",
volume = "129",
number = "4",
pages = "1667-1675",
doi = "10.1016/j.foodchem.2011.06.029"
}

Kozarski, M. S., Klaus, A., Nikšić, M., Jakovljević, D., Helsper, J. P. F. G.,& Van Griensven, L. J. L. D.. (2011). Antioxidative and immunomodulating activities of polysaccharide extracts of the medicinal mushrooms Agaricus bisporus, Agaricus brasiliensis, Ganoderma lucidum and Phellinus linteus. in Food Chemistry
Elsevier Sci Ltd, Oxford., 129(4), 1667-1675.
https://doi.org/10.1016/j.foodchem.2011.06.029

Kozarski MS, Klaus A, Nikšić M, Jakovljević D, Helsper JPFG, Van Griensven LJLD. Antioxidative and immunomodulating activities of polysaccharide extracts of the medicinal mushrooms Agaricus bisporus, Agaricus brasiliensis, Ganoderma lucidum and Phellinus linteus. in Food Chemistry. 2011;129(4):1667-1675.
doi:10.1016/j.foodchem.2011.06.029 .

Kozarski, Maja S., Klaus, Anita, Nikšić, Miomir, Jakovljević, Dragica, Helsper, Johannes P. F. G., Van Griensven, Leo J. L. D., "Antioxidative and immunomodulating activities of polysaccharide extracts of the medicinal mushrooms Agaricus bisporus, Agaricus brasiliensis, Ganoderma lucidum and Phellinus linteus" in Food Chemistry, 129, no. 4 (2011):1667-1675,
https://doi.org/10.1016/j.foodchem.2011.06.029 . .

TY  - JOUR
AU  - Soković, Marina
AU  - Vukojević, J
AU  - Marin, Petar D.
AU  - Brkić, D D
AU  - Vajs, Vlatka
AU  - Van Griensven, Leo J. L. D.
PY  - 2009
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/2779
AB  - The potential antifungal effects of Thymus vulgaris L., Thymus tosevii L., Mentha spicata L., and Mentha piperita L. (Labiatae) essential oils and their components against 17 micromycetal food poisoning, plant, animal and human pathogens are presented. The essential oils were obtained by hydrodestillation of dried plant material. Their composition was determined by GC-MS. Identification of individual constituents was made by comparison with analytical standards, and by computer matching mass spectral data with those of the Wiley/NBS Library of Mass Spectra. MIC's and MFC's of the oils and their components were determined by dilution assays. Thymol (48.9%) and p-cymene (19.0%) were the main components of T. vulgaris, while carvacrol (12.8%), α-terpinyl acetate (12.3%), cis-myrtanol (11.2%) and thymol (10.4%) were dominant in T. tosevii. Both Thymus species showed very strong antifungal activities. In M. piperita oil menthol (37.4%), menthyl acetate (17.4%) and menthone (12.7%) were the main components, whereas those of M. spicata oil were carvone (69.5%) and menthone (21.9%). Mentha sp. showed strong antifungal activities, however lower than Thymus sp. The commercial fungicide, bifonazole, used as a control, had much lower antifungal activity than the oils and components investigated. It is concluded that essential oils of Thymus and Mentha species possess great antifungal potential and could be used as natural preservatives and fungicides. © 2009 by the authors; licensee Molecular Diversity Preservation International.
PB  - MDPI
T2  - Molecules
T1  - Chemical composition of essential oils of Thymus and mentha species and their antifungal activities
VL  - 14
IS  - 1
SP  - 238
EP  - 249
DO  - 10.3390/molecules14010238
ER  - 

@article{
author = "Soković, Marina and Vukojević, J and Marin, Petar D. and Brkić, D D and Vajs, Vlatka and Van Griensven, Leo J. L. D.",
year = "2009",
abstract = "The potential antifungal effects of Thymus vulgaris L., Thymus tosevii L., Mentha spicata L., and Mentha piperita L. (Labiatae) essential oils and their components against 17 micromycetal food poisoning, plant, animal and human pathogens are presented. The essential oils were obtained by hydrodestillation of dried plant material. Their composition was determined by GC-MS. Identification of individual constituents was made by comparison with analytical standards, and by computer matching mass spectral data with those of the Wiley/NBS Library of Mass Spectra. MIC's and MFC's of the oils and their components were determined by dilution assays. Thymol (48.9%) and p-cymene (19.0%) were the main components of T. vulgaris, while carvacrol (12.8%), α-terpinyl acetate (12.3%), cis-myrtanol (11.2%) and thymol (10.4%) were dominant in T. tosevii. Both Thymus species showed very strong antifungal activities. In M. piperita oil menthol (37.4%), menthyl acetate (17.4%) and menthone (12.7%) were the main components, whereas those of M. spicata oil were carvone (69.5%) and menthone (21.9%). Mentha sp. showed strong antifungal activities, however lower than Thymus sp. The commercial fungicide, bifonazole, used as a control, had much lower antifungal activity than the oils and components investigated. It is concluded that essential oils of Thymus and Mentha species possess great antifungal potential and could be used as natural preservatives and fungicides. © 2009 by the authors; licensee Molecular Diversity Preservation International.",
publisher = "MDPI",
journal = "Molecules",
title = "Chemical composition of essential oils of Thymus and mentha species and their antifungal activities",
volume = "14",
number = "1",
pages = "238-249",
doi = "10.3390/molecules14010238"
}

Soković, M., Vukojević, J., Marin, P. D., Brkić, D. D., Vajs, V.,& Van Griensven, L. J. L. D.. (2009). Chemical composition of essential oils of Thymus and mentha species and their antifungal activities. in Molecules
MDPI., 14(1), 238-249.
https://doi.org/10.3390/molecules14010238

Soković M, Vukojević J, Marin PD, Brkić DD, Vajs V, Van Griensven LJLD. Chemical composition of essential oils of Thymus and mentha species and their antifungal activities. in Molecules. 2009;14(1):238-249.
doi:10.3390/molecules14010238 .

Soković, Marina, Vukojević, J, Marin, Petar D., Brkić, D D, Vajs, Vlatka, Van Griensven, Leo J. L. D., "Chemical composition of essential oils of Thymus and mentha species and their antifungal activities" in Molecules, 14, no. 1 (2009):238-249,
https://doi.org/10.3390/molecules14010238 . .